MD03G1286100.v1.1

WD repeat-containing protein 89 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009953
Physical Location & Seq
Reverse (-)
36555500 .. 36558263
2764 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1286100.v1.1.491

Sequence Viewer

Length: 1158 bp
ATGGAAGCCACGGACATGGAGGTGGAGGAACGACCACCGTCCAATCCTGATCCTTTCAAGCGTATCTCCCTCAAAAACTCCATCCAAACAAACTTCGGCGACGACTATGTCTTCCAAATCGTCCCCAAGGATGATTGGACGGCAATGGCAGTTTCGCTTTCGACCAACGCCGTGAAGGTGTACTCGCCCGTGACGGGCCAGTACTACGGAGAATGCAAGGGCCACTCTGCAACTATCAACCAAATTGCATTCTCTGGTCCATCAACTCCACATATCCTGCACTCTTGCTCATCTGATGGAACTATTCGAGCTTGGGACACGCGCACATTCCAACAGGTTTCATCTTTCCATTCCGGCTCTTCTCAAGAGATTTTCAGCTTTTCGTTCGGAGGATCAGGGAACAATCTTCTAGCCGCAGGGTGTGATACTCAGATACTATTCTGGGATTGGAGGAACGATAAACAAGTAGCATGTCTCGAGGACTCTCATGTAGAAGATGTTACCCAGGTTCACTTCATCCCTGATCATCAAAACAAGCTTCTTTCTGCTTCCATTGATGGATTGATGTGTATATTTGATACCGAAGGAGATATCAATGAAGACAATCATCTAGATTCAGTACTAAATGTTGGCACTTCGATTGGGAAGGTTGGGTTTTTTGGAGAGACATATCAAAAGCTTTGGTGTTTAACCCATATTGAGACCTTAAGTATCTGGGACTGGAAAGATGCAAGCGAAACGAGCTTCCAGGATGCTCGTTCTCTGGCTTCTAAAGGCTGGACACTCGACGATGTCGATTATTTTGTTGATTGCCACTACTCAAGAGAAGCAGAAAAGTTATGGGTAATTGGTGGCACTGATACTGGCACTTTAGGTTACTTCCCTGTAAGTTATGAAGGAAACAAAGCTATAGGGTCCGCTGAAGCAGTTCTTGGAGGTGGGCATACAGGTGTTGTTCGAAGCGTGTTGCCAATGTCGAGCACGCCCAGCGGATCCAGCCAAGGTCACGGCATTTTTGGATGGACTGGCGGTGAAGATGGCCGGTTATGTTGCTGGTTATCAGATAATGCACCCGAGATAAACCGATCATGGATTTCAAGCGCGCTGGTTTCAAGATCACCTAGGACCCGAAACACAACACGGCACCAACCCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010029 GO:0040008 GO:0048580 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:1900140 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

386

Amino Acids

42.39

Weight (kDa)

4.88

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 29 - 218 4.5e-13 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 34 - 154 5.1e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 45 - 112 3.7e-06 WDR3 first beta-propeller domain
WDR55 PF24796 46 - 245 7.2e-11 WDR55
Beta-prop_WDR5 PF25175 47 - 195 1.4e-23 WDR5 beta-propeller domain
WD40_Prp19 PF24814 49 - 186 1.1e-13 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 51 - 195 1.2e-18 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 55 - 155 2.2e-13 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 56 - 150 1.8e-09 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 92 - 203 6.3e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 95 - 197 4.9e-07 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 97 - 182 9.5e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EIPR1 PF23609 111 - 194 6.8e-06 EIPR1 beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 112 - 187 7.4e-06 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 121 - 195 2.2e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
AccB1I GGYRCC 1 cut(s) 1143
AccII CGCG 2 cut(s) 322, 1103
AciI CCGC 4 cut(s) 414, 918, 990, 1029
AclWI GGATC 4 cut(s) 44, 400, 987, 1000
AcoI YGGCCR 1 cut(s) 1039
AcuI CTGAAG 1 cut(s) 942
AfaI GTAC 3 cut(s) 182, 203, 621
AfiI CCNNNNNNNGG 1 cut(s) 194
AflII CTTAAG 1 cut(s) 706
AgsI TTSAA 3 cut(s) 58, 1098, 1113
AjnI CCWGG 2 cut(s) 504, 747
AjuI GAANNNNNNNTTGG 2 cut(s) 915, 947
AluBI AGCT 6 cut(s) 311, 378, 538, 679, 744, 908
AluI AGCT 6 cut(s) 311, 378, 538, 679, 744, 908
Alw21I GWGCWC 1 cut(s) 983
Alw26I GTCTC 3 cut(s) 479, 659, 695
AlwI GGATC 4 cut(s) 44, 400, 987, 1000
Ama87I CYCGRG 2 cut(s) 476, 1073
AoxI GGCC 3 cut(s) 196, 220, 1039
Asp700I GAANNNNTTC 1 cut(s) 927
AspA2I CCTAGG 1 cut(s) 1121
AspLEI GCGC 3 cut(s) 324, 1103, 1105
AspS9I GGNCC 5 cut(s) 196, 220, 257, 915, 1125
AsuHPI GGTGA 2 cut(s) 1043, 1110
AsuII TTCGAA 1 cut(s) 958
AvaI CYCGRG 2 cut(s) 476, 1073
AvaII GGWCC 3 cut(s) 257, 915, 1125
AvrII CCTAGG 1 cut(s) 1121
BamHI GGATCC 1 cut(s) 992
BanI GGYRCC 1 cut(s) 1143
BbsI GAAGAC 2 cut(s) 103, 606
Bbv12I GWGCWC 1 cut(s) 983
BccI CCATC 6 cut(s) 89, 268, 290, 551, 1014, 1031
BceAI ACGGC 3 cut(s) 155, 156, 1024
BciT130I CCWGG 2 cut(s) 506, 749
BclI TGATCA 1 cut(s) 523
BcoDI GTCTC 3 cut(s) 479, 659, 695
BfaI CTAG 4 cut(s) 410, 611, 1122, 1156
BfmI CTRYAG 1 cut(s) 909
BfrI CTTAAG 1 cut(s) 706
BisI GCNGC 1 cut(s) 414
BlnI CCTAGG 1 cut(s) 1121
BlsI GCNGC 1 cut(s) 415
BmcAI AGTACT 2 cut(s) 203, 621
Bme1390I CCNGG 2 cut(s) 506, 749
Bme18I GGWCC 3 cut(s) 257, 915, 1125
BmeT110I CYCGRG 2 cut(s) 476, 1073
BmgT120I GGNCC 5 cut(s) 196, 220, 257, 915, 1125
BmiI GGNNCC 4 cut(s) 916, 994, 1127, 1145
BmrFI CCNGG 2 cut(s) 506, 749
BmsI GCATC 2 cut(s) 718, 742
BoxI GACNNNNGTC 1 cut(s) 37
BpiI GAAGAC 2 cut(s) 103, 606
Bpu14I TTCGAA 1 cut(s) 958
BpuEI CTTGAG 2 cut(s) 348, 805
BsaI GGTCTC 1 cut(s) 695
BsaJI CCNNGG 5 cut(s) 9, 126, 504, 1000, 1121
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse118I RCCGGY 1 cut(s) 1041
Bse1I ACTGG 4 cut(s) 199, 725, 868, 1030
Bse3DI GCAATG 1 cut(s) 150
BseBI CCWGG 2 cut(s) 506, 749
BseDI CCNNGG 5 cut(s) 9, 126, 504, 1000, 1121
BseGI GGATG 5 cut(s) 81, 136, 516, 757, 1025
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 1 cut(s) 443
BseNI ACTGG 4 cut(s) 199, 725, 868, 1030
BsePI GCGCGC 1 cut(s) 1101
BseYI CCCAGC 1 cut(s) 986
BsgI GTGCAG 1 cut(s) 263
Bsh1236I CGCG 2 cut(s) 322, 1103
BshFI GGCC 3 cut(s) 198, 222, 1041
BshNI GGYRCC 1 cut(s) 1143
BsiHKAI GWGCWC 1 cut(s) 983
BsiHKCI CYCGRG 2 cut(s) 476, 1073
BsiSI CCGG 2 cut(s) 354, 1042
BslFI GGGAC 3 cut(s) 107, 329, 731
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 3 cut(s) 479, 659, 695
BsmFI GGGAC 3 cut(s) 107, 329, 731
BsmI GAATGC 2 cut(s) 218, 248
BsnI GGCC 3 cut(s) 198, 222, 1041
Bso31I GGTCTC 1 cut(s) 695
BsoBI CYCGRG 2 cut(s) 476, 1073
Bsp119I TTCGAA 1 cut(s) 958
Bsp1286I GDGCHC 1 cut(s) 983
Bsp143I GATC 6 cut(s) 49, 392, 523, 992, 1085, 1115
BspACI CCGC 4 cut(s) 414, 918, 990, 1029
BspANI GGCC 3 cut(s) 198, 222, 1041
BspCNI CTCAG 1 cut(s) 442
BspFNI CGCG 2 cut(s) 322, 1103
BspLI GGNNCC 4 cut(s) 916, 994, 1127, 1145
BspPI GGATC 4 cut(s) 44, 400, 987, 1000
BspQI GCTCTTC 1 cut(s) 364
BspT104I TTCGAA 1 cut(s) 958
BspT107I GGYRCC 1 cut(s) 1143
BspTI CTTAAG 1 cut(s) 706
BspTNI GGTCTC 1 cut(s) 695
BsrDI GCAATG 1 cut(s) 150
BsrFI RCCGGY 1 cut(s) 1041
BsrI ACTGG 4 cut(s) 199, 725, 868, 1030
BssAI RCCGGY 1 cut(s) 1041
BssECI CCNNGG 5 cut(s) 9, 126, 504, 1000, 1121
BssHII GCGCGC 1 cut(s) 1101
BssMI GATC 6 cut(s) 49, 392, 523, 992, 1085, 1115
BssT1I CCWWGG 3 cut(s) 126, 1000, 1121
Bst2UI CCWGG 2 cut(s) 506, 749
Bst4CI ACNGT 1 cut(s) 39
Bst6I CTCTTC 1 cut(s) 364
BstAFI CTTAAG 1 cut(s) 706
BstBI TTCGAA 1 cut(s) 958
BstC8I GCNNGC 3 cut(s) 733, 983, 1103
BstDEI CTNAG 1 cut(s) 429
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 5 cut(s) 81, 136, 516, 757, 1025
BstFNI CGCG 2 cut(s) 322, 1103
BstHHI GCGC 3 cut(s) 324, 1103, 1105
BstKTI GATC 6 cut(s) 52, 395, 526, 995, 1088, 1118
BstMAI GTCTC 3 cut(s) 479, 659, 695
BstMBI GATC 6 cut(s) 49, 392, 523, 992, 1085, 1115
BstMWI GCNNNNNNNGC 3 cut(s) 741, 987, 996
BstNI CCWGG 2 cut(s) 506, 749
BstNSI RCATGY 1 cut(s) 474
BstPAI GACNNNNGTC 1 cut(s) 37
BstSCI CCNGG 2 cut(s) 504, 747
BstSFI CTRYAG 1 cut(s) 909
BstUI CGCG 2 cut(s) 322, 1103
BstV2I GAAGAC 2 cut(s) 103, 606
BstX2I RGATCY 1 cut(s) 992
BstXI CCANNNNNNTGG 1 cut(s) 16
BstYI RGATCY 1 cut(s) 992
BsuRI GGCC 3 cut(s) 198, 222, 1041
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 5 cut(s) 81, 136, 516, 757, 1025
BtsIMutI CAGTG 1 cut(s) 855
Cac8I GCNNGC 3 cut(s) 733, 983, 1103
CfoI GCGC 3 cut(s) 324, 1103, 1105
Cfr10I RCCGGY 1 cut(s) 1041
Cfr13I GGNCC 5 cut(s) 196, 220, 257, 915, 1125
Csp6I GTAC 3 cut(s) 181, 202, 620
CviAII CATG 4 cut(s) 16, 471, 488, 1089
CviQI GTAC 3 cut(s) 181, 202, 620
DdeI CTNAG 1 cut(s) 429
DpnI GATC 6 cut(s) 51, 394, 525, 994, 1087, 1117
DpnII GATC 6 cut(s) 49, 392, 523, 992, 1085, 1115
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
EaeI YGGCCR 1 cut(s) 1039
Eam1104I CTCTTC 1 cut(s) 364
EarI CTCTTC 1 cut(s) 364
Eco130I CCWWGG 3 cut(s) 126, 1000, 1121
Eco31I GGTCTC 1 cut(s) 695
Eco32I GATATC 1 cut(s) 592
Eco47I GGWCC 3 cut(s) 257, 915, 1125
Eco57I CTGAAG 1 cut(s) 942
Eco88I CYCGRG 2 cut(s) 476, 1073
EcoO109I RGGNCCY 1 cut(s) 1125
EcoRII CCWGG 2 cut(s) 504, 747
EcoRV GATATC 1 cut(s) 592
EcoT14I CCWWGG 3 cut(s) 126, 1000, 1121
ErhI CCWWGG 3 cut(s) 126, 1000, 1121
FaeI CATG 4 cut(s) 19, 474, 491, 1092
FalI AAGNNNNNCTT 2 cut(s) 915, 947
FaqI GGGAC 3 cut(s) 107, 329, 731
FatI CATG 4 cut(s) 15, 470, 487, 1088
FbaI TGATCA 1 cut(s) 523
Fnu4HI GCNGC 1 cut(s) 414
FokI GGATG 5 cut(s) 68, 143, 503, 764, 1032
Fsp4HI GCNGC 1 cut(s) 414
FspBI CTAG 4 cut(s) 410, 611, 1122, 1156
GlaI GCGC 3 cut(s) 323, 1102, 1104
GluI GCNGC 1 cut(s) 414
GsaI CCCAGC 1 cut(s) 990
HaeIII GGCC 3 cut(s) 198, 222, 1041
HapII CCGG 2 cut(s) 354, 1042
HhaI GCGC 3 cut(s) 324, 1103, 1105
Hin1II CATG 4 cut(s) 19, 474, 491, 1092
Hin6I GCGC 3 cut(s) 322, 1101, 1103
HinP1I GCGC 3 cut(s) 322, 1101, 1103
HindIII AAGCTT 2 cut(s) 536, 677
HinfI GANTC 2 cut(s) 482, 614
HpaII CCGG 2 cut(s) 354, 1042
HphI GGTGA 2 cut(s) 1043, 1110
Hpy166II GTNNAC 2 cut(s) 181, 511
Hpy188I TCNGA 4 cut(s) 295, 389, 432, 1063
Hpy188III TCNNGA 6 cut(s) 47, 365, 476, 611, 822, 1113
Hpy8I GTNNAC 2 cut(s) 181, 511
Hpy99I CGWCG 2 cut(s) 104, 791
HpyAV CCTTC 4 cut(s) 169, 578, 640, 890
HpyCH4III ACNGT 1 cut(s) 39
HpyCH4V TGCA 6 cut(s) 216, 230, 248, 280, 731, 1070
HpyF10VI GCNNNNNNNGC 3 cut(s) 741, 987, 996
HpyF3I CTNAG 1 cut(s) 429
Hsp92II CATG 4 cut(s) 19, 474, 491, 1092
HspAI GCGC 3 cut(s) 322, 1101, 1103
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 6 cut(s) 49, 392, 523, 992, 1085, 1115
LguI GCTCTTC 1 cut(s) 364
LweI GCATC 2 cut(s) 718, 742
MaeI CTAG 4 cut(s) 410, 611, 1122, 1156
MaeIII GTNAC 4 cut(s) 190, 499, 875, 1004
MalI GATC 6 cut(s) 51, 394, 525, 994, 1087, 1117
MboI GATC 6 cut(s) 49, 392, 523, 992, 1085, 1115
MboII GAAGA 6 cut(s) 103, 351, 398, 506, 611, 1046
MflI RGATCY 1 cut(s) 992
MhlI GDGCHC 1 cut(s) 983
MluCI AATT 2 cut(s) 243, 846
MlyI GAGTC 1 cut(s) 476
MmeI TCCRAC 1 cut(s) 355
MnlI CCTC 7 cut(s) 13, 19, 80, 383, 444, 472, 929
MroXI GAANNNNTTC 1 cut(s) 927
MseI TTAA 2 cut(s) 689, 707
MslI CAYNNNNRTG 3 cut(s) 14, 20, 948
MspA1I CMGCKG 2 cut(s) 920, 990
MspCI CTTAAG 1 cut(s) 706
MspI CCGG 2 cut(s) 354, 1042
MspR9I CCNGG 2 cut(s) 506, 749
Mva1269I GAATGC 2 cut(s) 218, 248
MvaI CCWGG 2 cut(s) 506, 749
MvnI CGCG 2 cut(s) 322, 1103
MwoI GCNNNNNNNGC 3 cut(s) 741, 987, 996
NdeII GATC 6 cut(s) 49, 392, 523, 992, 1085, 1115
NlaIII CATG 4 cut(s) 19, 474, 491, 1092
NlaIV GGNNCC 4 cut(s) 916, 994, 1127, 1145
NmuCI GTSAC 2 cut(s) 190, 1004
NspI RCATGY 1 cut(s) 474
NspV TTCGAA 1 cut(s) 958
PaeR7I CTCGAG 1 cut(s) 476
PauI GCGCGC 1 cut(s) 1101
PciSI GCTCTTC 1 cut(s) 364
PcsI WCGNNNNNNNCGW 1 cut(s) 792
PctI GAATGC 2 cut(s) 218, 248
PdmI GAANNNNTTC 1 cut(s) 927
PfeI GAWTC 1 cut(s) 614
PflFI GACNNNGTC 2 cut(s) 107, 791
PfoI TCCNGGA 1 cut(s) 747
PkrI GCNGC 1 cut(s) 415
PleI GAGTC 1 cut(s) 476
PpsI GAGTC 1 cut(s) 476
PpuMI RGGWCCY 1 cut(s) 1125
PshAI GACNNNNGTC 1 cut(s) 37
Psp5II RGGWCCY 1 cut(s) 1125
Psp6I CCWGG 2 cut(s) 504, 747
PspFI CCCAGC 1 cut(s) 986
PspGI CCWGG 2 cut(s) 504, 747
PspN4I GGNNCC 4 cut(s) 916, 994, 1127, 1145
PspPI GGNCC 5 cut(s) 196, 220, 257, 915, 1125
PspPPI RGGWCCY 1 cut(s) 1125
PsuI RGATCY 1 cut(s) 992
PsyI GACNNNGTC 2 cut(s) 107, 791
PteI GCGCGC 1 cut(s) 1101
RsaI GTAC 3 cut(s) 182, 203, 621
RsaNI GTAC 3 cut(s) 181, 202, 620
RseI CAYNNNNRTG 3 cut(s) 14, 20, 948
SapI GCTCTTC 1 cut(s) 364
SaqAI TTAA 2 cut(s) 689, 707
SatI GCNGC 1 cut(s) 414
Sau3AI GATC 6 cut(s) 49, 392, 523, 992, 1085, 1115
Sau96I GGNCC 5 cut(s) 196, 220, 257, 915, 1125
ScaI AGTACT 2 cut(s) 203, 621
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 2 cut(s) 506, 749
SduI GDGCHC 1 cut(s) 983
SfaNI GCATC 2 cut(s) 718, 742
SfcI CTRYAG 1 cut(s) 909
Sfr274I CTCGAG 1 cut(s) 476
SfuI TTCGAA 1 cut(s) 958
SinI GGWCC 3 cut(s) 257, 915, 1125
SlaI CTCGAG 1 cut(s) 476
SmiMI CAYNNNNRTG 3 cut(s) 14, 20, 948
SmlI CTYRAG 4 cut(s) 363, 476, 706, 820
SmoI CTYRAG 4 cut(s) 363, 476, 706, 820
Sse9I AATT 2 cut(s) 243, 846
SsiI CCGC 4 cut(s) 414, 918, 990, 1029
SspMI CTAG 4 cut(s) 410, 611, 1122, 1156
StyD4I CCNGG 2 cut(s) 504, 747
StyI CCWWGG 3 cut(s) 126, 1000, 1121
TaaI ACNGT 1 cut(s) 39
TaqI TCGA 8 cut(s) 161, 307, 477, 638, 786, 795, 958, 977
TasI AATT 2 cut(s) 243, 846
TatI WGTACW 3 cut(s) 180, 201, 619
TauI GCSGC 1 cut(s) 416
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 2 cut(s) 689, 707
Tru9I TTAA 2 cut(s) 689, 707
TscAI CASTG 1 cut(s) 862
TseFI GTSAC 2 cut(s) 190, 1004
Tsp45I GTSAC 2 cut(s) 190, 1004
TspDTI ATGAA 4 cut(s) 330, 505, 612, 909
TspGWI ACGGA 2 cut(s) 26, 222
TspRI CASTG 1 cut(s) 862
Tth111I GACNNNGTC 2 cut(s) 107, 791
Vha464I CTTAAG 1 cut(s) 706
VpaK11BI GGWCC 3 cut(s) 257, 915, 1125
XbaI TCTAGA 1 cut(s) 610
XceI RCATGY 1 cut(s) 474
XhoI CTCGAG 1 cut(s) 476
XmaJI CCTAGG 1 cut(s) 1121
XmnI GAANNNNTTC 1 cut(s) 927
XspI CTAG 4 cut(s) 410, 611, 1122, 1156
ZrmI AGTACT 2 cut(s) 203, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.