Rh6DG351800

WD repeat-containing protein 89 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0009953
Physical Location & Seq
Reverse (-)
55162226 .. 55164529
2304 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG351800.1

Sequence Viewer

Length: 1146 bp
ATGGACTTGGATCAGTTGGAGGCAAAACAGTCCAATCCAAACTCATTCAAGCGTTTCGGCCTCAAAACCTCTATCCCAACCAACTTCGGAGACGATTACGTTTTCCAAATTGTGCCCAAGGATGATTGGACAGCAATGGCGGTGTCACTGTCAACGAACGCAGTGAAGCTCTACTCACCGGTGACTGGTCAGTACTATGGAGAGTGCAAAGGCCACTCTGCAACTATCAATCAAATTTCATTTGCAGACCCATCAGCCCCACATGTTTTGCACTCTTGCTCTTCCGACGGAACCATTAGACTTTGGGACTCCCGCACATTCCAACAGGTGTCATCTTTTCACTCTGGTTCGAGTGAGGAGATTTTCAGCTTTTCGTTTGGTGGATCATCTAATAATCTTCTGGCCGCAGGATGTAATTCTCAGATACTAATGTGGGATTGGAGGAACGACAAACAAGTAGCATGCTTAGAGGACTCTCATGTGGAAGATGTTACTCAGGTTCACTTCATTCCTGACCATCAAAGCAAGCTCCTTTCTGCTTCTGTAGATGGATTGATATGTATATTTGATACAGATGGAGATATCAACGAAGATGATCATCTTGAGTCAGTGCTAAATGTGGGCACTTCCATTGGGAAGGTGGGGTTTTTTGGTGAGACGTATCAAAAGCTTTGGTGTTTGACCCATATTGAAACCTTAAGTATCTGGGACTGGAAGGATGCAACTGAAACAAATTTCCAGGACGCTCGTTCGTTGGCTTCTAACAGTTGGACTCTAGGTGAAGTTGATTATTTTGTTGATTGCCACTACTCAAGAGAAGCAGAACGATTATGGCTTATTGGTGGCGCCAACACTGGCACTTTAGGTTACTTCCCTGTAACTTACACAGGAACGCGAGCCATAGGTTCCCCTGAAGCAATTATGGAAGGTGGCCATACGGGTGTTGTCCGAAGTGTGTTGCCTATGTCAAACATGCCAGGTCCCCAAGGCCAAAGCATTTTTGGATGGACTGGTGGTGAAGATGGCCATTTATGTTGCTGGTTATCAGATGGTTCTCTGGACACCATAAACCGATCATGGAGTTCAAGCGAACTGGTTTTGAGATCACCAAAGATCCGAAAGTCAAAAAGGCATAGTCCCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010029 GO:0040008 GO:0048580 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:1900140 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

381

Amino Acids

42.13

Weight (kDa)

4.97

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 31 - 240 3.4e-12 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 34 - 192 4.3e-10 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 44 - 192 3.4e-23 WDR5 beta-propeller domain
WDR55 PF24796 45 - 240 1.4e-09 WDR55
Beta-prop_WDR3_1st PF25173 48 - 192 1.5e-16 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 49 - 183 4.6e-12 Prp19 WD40 domain
Beta-prop_WDR75_1st PF23869 49 - 104 6.5e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_THOC3 PF25174 53 - 152 2.2e-11 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 53 - 147 8.1e-08 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 59 - 247 9.4e-06 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 70 - 194 1.7e-07 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 84 - 200 7.8e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 90 - 192 4e-06 WDR90/POC16, second beta-propeller
Beta-prop_EML PF23409 94 - 179 1e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EIPR1 PF23609 105 - 191 3e-06 EIPR1 beta-propeller
Beta-prop_THOC3 PF25174 121 - 192 8.2e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 845
AccII CGCG 1 cut(s) 895
AciI CCGC 3 cut(s) 140, 313, 405
AclWI GGATC 3 cut(s) 18, 391, 1108
AcoI YGGCCR 3 cut(s) 402, 931, 1024
AcsI RAATTY 2 cut(s) 234, 733
AcuI CTGAAG 1 cut(s) 933
AcyI GRCGYC 1 cut(s) 846
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 1 cut(s) 185
AflII CTTAAG 1 cut(s) 697
AflIII ACRYGT 1 cut(s) 262
AgeI ACCGGT 1 cut(s) 178
AgsI TTSAA 3 cut(s) 49, 692, 1086
AjnI CCWGG 2 cut(s) 738, 976
AluBI AGCT 4 cut(s) 169, 369, 529, 670
AluI AGCT 4 cut(s) 169, 369, 529, 670
Alw26I GTCTC 2 cut(s) 84, 650
AlwI GGATC 3 cut(s) 18, 391, 1108
AoxI GGCC 6 cut(s) 58, 211, 402, 931, 988, 1024
ApoI RAATTY 2 cut(s) 234, 733
AsiGI ACCGGT 1 cut(s) 178
AspLEI GCGC 1 cut(s) 848
AspS9I GGNCC 1 cut(s) 980
AsuHPI GGTGA 6 cut(s) 168, 193, 665, 791, 1028, 1098
AvaII GGWCC 1 cut(s) 980
BaeGI GKGCMC 2 cut(s) 117, 626
BaeI ACNNNNGTAYC 2 cut(s) 416, 449
BalI TGGCCA 2 cut(s) 933, 1026
BanI GGYRCC 1 cut(s) 845
BccI CCATC 7 cut(s) 259, 525, 542, 569, 999, 1016, 1043
BciT130I CCWGG 2 cut(s) 740, 978
BclI TGATCA 1 cut(s) 595
BcoDI GTCTC 2 cut(s) 84, 650
BfaI CTAG 2 cut(s) 776, 1144
BfmI CTRYAG 1 cut(s) 543
BfoI RGCGCY 1 cut(s) 849
BfrI CTTAAG 1 cut(s) 697
BisI GCNGC 1 cut(s) 405
BlsI GCNGC 1 cut(s) 406
BmcAI AGTACT 1 cut(s) 194
Bme1390I CCNGG 2 cut(s) 740, 978
Bme18I GGWCC 1 cut(s) 980
BmgT120I GGNCC 1 cut(s) 980
BmiI GGNNCC 4 cut(s) 292, 847, 907, 982
BmrFI CCNGG 2 cut(s) 740, 978
BmsI GCATC 1 cut(s) 709
BpuEI CTTGAG 2 cut(s) 623, 796
BsaBI GATNNNNATC 1 cut(s) 597
BsaHI GRCGYC 1 cut(s) 846
BsaJI CCNNGG 2 cut(s) 117, 985
BsaWI WCCGGW 1 cut(s) 178
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 5 cut(s) 190, 716, 859, 1015, 1098
Bse3DI GCAATG 1 cut(s) 141
Bse8I GATNNNNATC 1 cut(s) 597
BseBI CCWGG 2 cut(s) 740, 978
BseDI CCNNGG 2 cut(s) 117, 985
BseGI GGATG 4 cut(s) 127, 416, 724, 1010
BseJI GATNNNNATC 1 cut(s) 597
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMI GCAATG 1 cut(s) 141
BseMII CTCAG 2 cut(s) 434, 509
BseNI ACTGG 5 cut(s) 190, 716, 859, 1015, 1098
BseRI GAGGAG 1 cut(s) 371
BseSI GKGCMC 2 cut(s) 117, 626
Bsh1236I CGCG 1 cut(s) 895
BshFI GGCC 6 cut(s) 60, 213, 404, 933, 990, 1026
BshNI GGYRCC 1 cut(s) 845
BshTI ACCGGT 1 cut(s) 178
BsiSI CCGG 1 cut(s) 179
BslFI GGGAC 4 cut(s) 320, 722, 966, 1122
BslI CCNNNNNNNGG 1 cut(s) 185
BsmAI GTCTC 2 cut(s) 84, 650
BsmBI CGTCTC 2 cut(s) 84, 650
BsmFI GGGAC 4 cut(s) 320, 722, 966, 1122
BsnI GGCC 6 cut(s) 60, 213, 404, 933, 990, 1026
Bsp1286I GDGCHC 2 cut(s) 117, 626
Bsp143I GATC 6 cut(s) 10, 383, 595, 1073, 1103, 1113
BspACI CCGC 3 cut(s) 140, 313, 405
BspANI GGCC 6 cut(s) 60, 213, 404, 933, 990, 1026
BspCNI CTCAG 2 cut(s) 433, 508
BspFNI CGCG 1 cut(s) 895
BspLI GGNNCC 4 cut(s) 292, 847, 907, 982
BspPI GGATC 3 cut(s) 18, 391, 1108
BspQI GCTCTTC 1 cut(s) 286
BspT107I GGYRCC 1 cut(s) 845
BspTI CTTAAG 1 cut(s) 697
BsrDI GCAATG 1 cut(s) 141
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 5 cut(s) 190, 716, 859, 1015, 1098
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 2 cut(s) 117, 985
BssMI GATC 6 cut(s) 10, 383, 595, 1073, 1103, 1113
BssNI GRCGYC 1 cut(s) 846
BssT1I CCWWGG 2 cut(s) 117, 985
Bst2UI CCWGG 2 cut(s) 740, 978
Bst4CI ACNGT 3 cut(s) 30, 150, 767
Bst6I CTCTTC 1 cut(s) 286
BstACI GRCGYC 1 cut(s) 846
BstAFI CTTAAG 1 cut(s) 697
BstC8I GCNNGC 3 cut(s) 463, 527, 897
BstDEI CTNAG 3 cut(s) 420, 466, 495
BstF5I GGATG 4 cut(s) 127, 416, 724, 1010
BstFNI CGCG 1 cut(s) 895
BstH2I RGCGCY 1 cut(s) 849
BstHHI GCGC 1 cut(s) 848
BstKTI GATC 6 cut(s) 13, 386, 598, 1076, 1106, 1116
BstMAI GTCTC 2 cut(s) 84, 650
BstMBI GATC 6 cut(s) 10, 383, 595, 1073, 1103, 1113
BstNI CCWGG 2 cut(s) 740, 978
BstNSI RCATGY 3 cut(s) 266, 465, 976
BstSCI CCNGG 2 cut(s) 738, 976
BstSFI CTRYAG 1 cut(s) 543
BstSLI GKGCMC 2 cut(s) 117, 626
BstUI CGCG 1 cut(s) 895
BstX2I RGATCY 1 cut(s) 1113
BstYI RGATCY 1 cut(s) 1113
BsuRI GGCC 6 cut(s) 60, 213, 404, 933, 990, 1026
BtsCI GGATG 4 cut(s) 127, 416, 724, 1010
BtsI GCAGTG 1 cut(s) 168
BtsIMutI CAGTG 4 cut(s) 146, 168, 615, 852
Cac8I GCNNGC 3 cut(s) 463, 527, 897
CfoI GCGC 1 cut(s) 848
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 980
CseI GACGC 1 cut(s) 752
Csp6I GTAC 1 cut(s) 193
CspAI ACCGGT 1 cut(s) 178
CspCI CAANNNNNGTGG 2 cut(s) 249, 284
CviAII CATG 5 cut(s) 263, 462, 479, 973, 1077
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 3 cut(s) 420, 466, 495
DinI GGCGCC 1 cut(s) 847
DpnI GATC 6 cut(s) 12, 385, 597, 1075, 1105, 1115
DpnII GATC 6 cut(s) 10, 383, 595, 1073, 1103, 1113
EaeI YGGCCR 3 cut(s) 402, 931, 1024
Eam1104I CTCTTC 1 cut(s) 286
EarI CTCTTC 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 117, 985
Eco32I GATATC 1 cut(s) 583
Eco47I GGWCC 1 cut(s) 980
Eco57I CTGAAG 1 cut(s) 933
EcoO109I RGGNCCY 1 cut(s) 980
EcoRII CCWGG 2 cut(s) 738, 976
EcoRV GATATC 1 cut(s) 583
EcoT14I CCWWGG 2 cut(s) 117, 985
EgeI GGCGCC 1 cut(s) 847
EheI GGCGCC 1 cut(s) 847
ErhI CCWWGG 2 cut(s) 117, 985
Esp3I CGTCTC 2 cut(s) 84, 650
FaeI CATG 5 cut(s) 266, 465, 482, 976, 1080
FaqI GGGAC 4 cut(s) 320, 722, 966, 1122
FatI CATG 5 cut(s) 262, 461, 478, 972, 1076
FauI CCCGC 1 cut(s) 320
FbaI TGATCA 1 cut(s) 595
Fnu4HI GCNGC 1 cut(s) 405
FokI GGATG 4 cut(s) 134, 423, 731, 1017
Fsp4HI GCNGC 1 cut(s) 405
FspBI CTAG 2 cut(s) 776, 1144
GlaI GCGC 1 cut(s) 847
GluI GCNGC 1 cut(s) 405
HaeII RGCGCY 1 cut(s) 849
HaeIII GGCC 6 cut(s) 60, 213, 404, 933, 990, 1026
HapII CCGG 1 cut(s) 179
HgaI GACGC 1 cut(s) 752
HhaI GCGC 1 cut(s) 848
Hin1I GRCGYC 1 cut(s) 846
Hin1II CATG 5 cut(s) 266, 465, 482, 976, 1080
Hin6I GCGC 1 cut(s) 846
HinP1I GCGC 1 cut(s) 846
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 4 cut(s) 308, 473, 605, 772
HpaII CCGG 1 cut(s) 179
HphI GGTGA 6 cut(s) 168, 193, 665, 791, 1028, 1098
Hpy166II GTNNAC 2 cut(s) 153, 502
Hpy188I TCNGA 6 cut(s) 89, 286, 423, 950, 1048, 1118
Hpy188III TCNNGA 4 cut(s) 512, 602, 813, 1058
Hpy8I GTNNAC 2 cut(s) 153, 502
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 3 cut(s) 631, 709, 920
HpyCH4III ACNGT 3 cut(s) 30, 150, 767
HpyCH4IV ACGT 2 cut(s) 99, 659
HpyCH4V TGCA 5 cut(s) 207, 221, 245, 271, 722
HpyF3I CTNAG 3 cut(s) 420, 466, 495
HpySE526I ACGT 2 cut(s) 99, 659
Hsp92I GRCGYC 1 cut(s) 846
Hsp92II CATG 5 cut(s) 266, 465, 482, 976, 1080
HspAI GCGC 1 cut(s) 846
KasI GGCGCC 1 cut(s) 845
Ksp22I TGATCA 1 cut(s) 595
Kzo9I GATC 6 cut(s) 10, 383, 595, 1073, 1103, 1113
LguI GCTCTTC 1 cut(s) 286
LmnI GCTCC 1 cut(s) 534
LweI GCATC 1 cut(s) 709
MaeI CTAG 2 cut(s) 776, 1144
MaeII ACGT 2 cut(s) 99, 659
MaeIII GTNAC 5 cut(s) 144, 181, 490, 866, 877
MalI GATC 6 cut(s) 12, 385, 597, 1075, 1105, 1115
MboI GATC 6 cut(s) 10, 383, 595, 1073, 1103, 1113
MboII GAAGA 5 cut(s) 273, 389, 497, 602, 1031
MflI RGATCY 1 cut(s) 1113
MhlI GDGCHC 2 cut(s) 117, 626
MlsI TGGCCA 2 cut(s) 933, 1026
MluCI AATT 5 cut(s) 108, 234, 415, 733, 918
MluNI TGGCCA 2 cut(s) 933, 1026
Mly113I GGCGCC 1 cut(s) 846
MlyI GAGTC 4 cut(s) 302, 467, 614, 766
MmeI TCCRAC 3 cut(s) 309, 346, 749
MnlI CCTC 6 cut(s) 13, 71, 79, 349, 435, 463
Mox20I TGGCCA 2 cut(s) 933, 1026
MscI TGGCCA 2 cut(s) 933, 1026
MseI TTAA 1 cut(s) 698
MslI CAYNNNNRTG 1 cut(s) 939
Msp20I TGGCCA 2 cut(s) 933, 1026
MspCI CTTAAG 1 cut(s) 697
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 2 cut(s) 740, 978
MvaI CCWGG 2 cut(s) 740, 978
MvnI CGCG 1 cut(s) 895
NarI GGCGCC 1 cut(s) 846
NdeII GATC 6 cut(s) 10, 383, 595, 1073, 1103, 1113
NlaIII CATG 5 cut(s) 266, 465, 482, 976, 1080
NlaIV GGNNCC 4 cut(s) 292, 847, 907, 982
NmuCI GTSAC 2 cut(s) 144, 181
NspI RCATGY 3 cut(s) 266, 465, 976
PaeI GCATGC 1 cut(s) 465
PciI ACATGT 1 cut(s) 262
PciSI GCTCTTC 1 cut(s) 286
PfoI TCCNGGA 1 cut(s) 738
PinAI ACCGGT 1 cut(s) 178
PkrI GCNGC 1 cut(s) 406
PleI GAGTC 4 cut(s) 302, 467, 613, 766
PluTI GGCGCC 1 cut(s) 849
PpsI GAGTC 4 cut(s) 302, 467, 613, 766
PpuMI RGGWCCY 1 cut(s) 980
PscI ACATGT 1 cut(s) 262
Psp5II RGGWCCY 1 cut(s) 980
Psp6I CCWGG 2 cut(s) 738, 976
PspGI CCWGG 2 cut(s) 738, 976
PspN4I GGNNCC 4 cut(s) 292, 847, 907, 982
PspPI GGNCC 1 cut(s) 980
PspPPI RGGWCCY 1 cut(s) 980
PsuI RGATCY 1 cut(s) 1113
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 1 cut(s) 939
SapI GCTCTTC 1 cut(s) 286
SaqAI TTAA 1 cut(s) 698
SatI GCNGC 1 cut(s) 405
Sau3AI GATC 6 cut(s) 10, 383, 595, 1073, 1103, 1113
Sau96I GGNCC 1 cut(s) 980
ScaI AGTACT 1 cut(s) 194
SchI GAGTC 4 cut(s) 302, 467, 614, 766
ScrFI CCNGG 2 cut(s) 740, 978
SduI GDGCHC 2 cut(s) 117, 626
SfaNI GCATC 1 cut(s) 709
SfcI CTRYAG 1 cut(s) 543
SfoI GGCGCC 1 cut(s) 847
SgrAI CRCCGGYG 1 cut(s) 178
SinI GGWCC 1 cut(s) 980
SmiMI CAYNNNNRTG 1 cut(s) 939
SmlI CTYRAG 3 cut(s) 602, 697, 811
SmoI CTYRAG 3 cut(s) 602, 697, 811
SphI GCATGC 1 cut(s) 465
Sse9I AATT 5 cut(s) 108, 234, 415, 733, 918
SsiI CCGC 3 cut(s) 140, 313, 405
SspDI GGCGCC 1 cut(s) 845
SspMI CTAG 2 cut(s) 776, 1144
StyD4I CCNGG 2 cut(s) 738, 976
StyI CCWWGG 2 cut(s) 117, 985
TaaI ACNGT 3 cut(s) 30, 150, 767
TaiI ACGT 2 cut(s) 102, 662
TaqI TCGA 1 cut(s) 350
TasI AATT 5 cut(s) 108, 234, 415, 733, 918
TatI WGTACW 1 cut(s) 192
TauI GCSGC 1 cut(s) 407
Tru1I TTAA 1 cut(s) 698
Tru9I TTAA 1 cut(s) 698
TscAI CASTG 4 cut(s) 153, 168, 615, 859
TseFI GTSAC 2 cut(s) 144, 181
Tsp45I GTSAC 2 cut(s) 144, 181
TspDTI ATGAA 2 cut(s) 228, 496
TspGWI ACGGA 1 cut(s) 303
TspRI CASTG 4 cut(s) 153, 168, 615, 859
Vha464I CTTAAG 1 cut(s) 697
VpaK11BI GGWCC 1 cut(s) 980
XapI RAATTY 2 cut(s) 234, 733
XceI RCATGY 3 cut(s) 266, 465, 976
XcmI CCANNNNNNNNNTGG 2 cut(s) 637, 998
XspI CTAG 2 cut(s) 776, 1144
ZrmI AGTACT 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.