MD11G1306000.v1.1

WD repeat-containing protein 89 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009953
Physical Location & Seq
Reverse (-)
42058908 .. 42061754
2847 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1306000.v1.1.491

Sequence Viewer

Length: 1158 bp
ATGGAAGCTACGGACATGGAGGTGGAGGAACTCCCTCCGTCCAATCCTGACCCCTTCAAGCGTATCTCCCTAAAAAATTCCATCCAAACCAACTTCGGCGACGACTATGTCTTCCAAATTGTCCCCAAGGATGATTGGACGGCAATGGCGGTGTCGCTTTCGACCAACGCCGTGAAGGTGTACTCTCCGGTGACGGGCCAGTACTACGGAGAGTGCAATGGCCACTCTGCCACTATCAACCAAATTGCATTCTCTGGTCCATCAACTCCACATGTCCTGCACTCTTGCTCTTCTGATGGAACGATTCGAGCTTGGGACACGCGCACATTTCAACAGGTTTCATCTTTTCATTCTGGCTCTTCTCTGGAGATTTTCAGCTTTTCGTTCGGAGGATCAGGGAACAATCTTCTCGCTGCGGGATGTGATGCTCAGATACTATTCTGGGATTGGAGGAGCAATAAACAGGTAGCATGTCTGGAGGATTCTCATGTAGAAGATGTTACCCAGGTTCACTTCATCCCTGATCATCAAAGCAAGCTTCTTTCTGCTTCTGTTGATGGATTGATGTGTATATTTGATACCAAAGGAGATATCAATGAAGACGATCATCTAGATTCAGTGCTAAACGTTGGCACTTCAATTGGGAAGGTTGGGTTTTTTGGAGAGACGAATGAAAAGCTCTGGTGTTTGACCCATATTGAGACCTTAAGTATCTGGGACTGGAAGGATGCAAGCGAAACGAGTTTCCAGGATGCTCGTTCTTTGGCTTCTAAAGGCTGGACACTCGATGATGTCGATTATTTTATTGATTGCCACTACTCAAGAGAAGCAGAACAGTTGTGGGTAATTGGTGGCACTAATAGTGGCACTTTAGGTTACTTCCCTGTAAGTTATAAAGGAAACAAAGCTATAGGGTCCGCTGAAGCAGTTCTTGGAGGTGGACACACAGGCATTGTTCGAAGTGTGTTGCCAATGTCAAGCATGCCCAGCGGATCCAGCCAAGGCCACGGCATTTTTGGATGGACTGGTGGTGAAGATGGCCGCTTATGTTGCTGGTTATCAGATAATGCTCCCGAGATAAACCGATCATGGATTTCGAGCACACTGGTTGCAAGATCACCTCGGACCCGCAACACAAAACGGCATCAACCCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010029 GO:0040008 GO:0048580 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:1900140 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

386

Amino Acids

42.24

Weight (kDa)

4.97

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 29 - 225 3.1e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 37 - 109 3.7e-06 WDR3 first beta-propeller domain
WDR55 PF24796 46 - 246 9.3e-11 WDR55
Beta-prop_WDR5 PF25175 47 - 196 2e-23 WDR5 beta-propeller domain
WD40_Prp19 PF24814 49 - 186 2.3e-13 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 51 - 195 5.1e-17 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 55 - 155 9.7e-13 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 56 - 149 5.8e-09 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 62 - 249 2.2e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 87 - 201 2.6e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 95 - 198 2.8e-07 G protein beta WD-40 repeat protein
Beta-prop_EML PF23409 96 - 182 4.6e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 124 - 195 1.5e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 894
AasI GACNNNNNNGTC 1 cut(s) 107
AccII CGCG 1 cut(s) 322
AciI CCGC 6 cut(s) 149, 416, 918, 990, 1042, 1129
AclI AACGTT 1 cut(s) 627
AclWI GGATC 3 cut(s) 400, 987, 1000
AcoI YGGCCR 2 cut(s) 220, 1039
AcsI RAATTY 1 cut(s) 76
AcuI CTGAAG 1 cut(s) 942
AfaI GTAC 2 cut(s) 182, 203
AfiI CCNNNNNNNGG 1 cut(s) 194
AflII CTTAAG 1 cut(s) 706
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 3 cut(s) 58, 332, 639
AjnI CCWGG 2 cut(s) 504, 747
AjuI GAANNNNNNNTTGG 2 cut(s) 915, 947
AluBI AGCT 6 cut(s) 8, 311, 378, 538, 679, 908
AluI AGCT 6 cut(s) 8, 311, 378, 538, 679, 908
Alw21I GWGCWC 1 cut(s) 1103
Alw26I GTCTC 2 cut(s) 659, 695
AlwI GGATC 3 cut(s) 400, 987, 1000
Ama87I CYCGRG 1 cut(s) 1073
AoxI GGCC 4 cut(s) 196, 220, 1003, 1039
ApeKI GCWGC 1 cut(s) 413
ApoI RAATTY 1 cut(s) 76
Asp700I GAANNNNTTC 1 cut(s) 927
AspLEI GCGC 1 cut(s) 324
AspS9I GGNCC 4 cut(s) 196, 257, 915, 1125
AsuHPI GGTGA 3 cut(s) 202, 1043, 1110
AsuII TTCGAA 1 cut(s) 958
AvaI CYCGRG 1 cut(s) 1073
AvaII GGWCC 3 cut(s) 257, 915, 1125
BalI TGGCCA 1 cut(s) 222
BamHI GGATCC 1 cut(s) 992
BbsI GAAGAC 2 cut(s) 103, 606
Bbv12I GWGCWC 1 cut(s) 1103
BbvI GCAGC 1 cut(s) 400
BccI CCATC 6 cut(s) 89, 268, 290, 551, 1014, 1031
BceAI ACGGC 3 cut(s) 155, 156, 1024
BciT130I CCWGG 2 cut(s) 506, 749
BclI TGATCA 1 cut(s) 523
BcoDI GTCTC 2 cut(s) 659, 695
BfaI CTAG 2 cut(s) 611, 1156
BfmI CTRYAG 1 cut(s) 909
BfrI CTTAAG 1 cut(s) 706
BisI GCNGC 2 cut(s) 414, 1042
BlsI GCNGC 2 cut(s) 415, 1043
BmcAI AGTACT 1 cut(s) 203
Bme1390I CCNGG 2 cut(s) 506, 749
Bme18I GGWCC 3 cut(s) 257, 915, 1125
BmeT110I CYCGRG 1 cut(s) 1073
BmgT120I GGNCC 4 cut(s) 196, 257, 915, 1125
BmiI GGNNCC 3 cut(s) 916, 994, 1127
BmrFI CCNGG 2 cut(s) 506, 749
BmsI GCATC 4 cut(s) 415, 718, 742, 1153
BpiI GAAGAC 2 cut(s) 103, 606
BpmI CTGGAG 2 cut(s) 386, 497
Bpu14I TTCGAA 1 cut(s) 958
BpuEI CTTGAG 1 cut(s) 805
BsaI GGTCTC 1 cut(s) 695
BsaJI CCNNGG 5 cut(s) 126, 504, 1000, 1006, 1121
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse1I ACTGG 4 cut(s) 199, 725, 1030, 1110
Bse3DI GCAATG 2 cut(s) 150, 223
BseBI CCWGG 2 cut(s) 506, 749
BseDI CCNNGG 5 cut(s) 126, 504, 1000, 1006, 1121
BseGI GGATG 7 cut(s) 81, 136, 425, 516, 733, 757, 1025
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 2 cut(s) 150, 223
BseMII CTCAG 1 cut(s) 443
BseNI ACTGG 4 cut(s) 199, 725, 1030, 1110
BseRI GAGGAG 1 cut(s) 466
BseXI GCAGC 1 cut(s) 400
BseYI CCCAGC 1 cut(s) 986
BsgI GTGCAG 1 cut(s) 263
Bsh1236I CGCG 1 cut(s) 322
BshFI GGCC 4 cut(s) 198, 222, 1005, 1041
BsiHKAI GWGCWC 1 cut(s) 1103
BsiHKCI CYCGRG 1 cut(s) 1073
BsiSI CCGG 1 cut(s) 188
BslFI GGGAC 3 cut(s) 107, 329, 731
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 2 cut(s) 659, 695
BsmBI CGTCTC 1 cut(s) 659
BsmFI GGGAC 3 cut(s) 107, 329, 731
BsmI GAATGC 1 cut(s) 248
BsnI GGCC 4 cut(s) 198, 222, 1005, 1041
Bso31I GGTCTC 1 cut(s) 695
BsoBI CYCGRG 1 cut(s) 1073
Bsp119I TTCGAA 1 cut(s) 958
Bsp1286I GDGCHC 1 cut(s) 1103
Bsp143I GATC 6 cut(s) 392, 523, 604, 992, 1085, 1115
BspACI CCGC 6 cut(s) 149, 416, 918, 990, 1042, 1129
BspANI GGCC 4 cut(s) 198, 222, 1005, 1041
BspCNI CTCAG 1 cut(s) 442
BspFNI CGCG 1 cut(s) 322
BspLI GGNNCC 3 cut(s) 916, 994, 1127
BspPI GGATC 3 cut(s) 400, 987, 1000
BspQI GCTCTTC 2 cut(s) 295, 364
BspT104I TTCGAA 1 cut(s) 958
BspTI CTTAAG 1 cut(s) 706
BspTNI GGTCTC 1 cut(s) 695
BsrDI GCAATG 2 cut(s) 150, 223
BsrI ACTGG 4 cut(s) 199, 725, 1030, 1110
BssECI CCNNGG 5 cut(s) 126, 504, 1000, 1006, 1121
BssMI GATC 6 cut(s) 392, 523, 604, 992, 1085, 1115
BssT1I CCWWGG 2 cut(s) 126, 1000
Bst2UI CCWGG 2 cut(s) 506, 749
Bst4CI ACNGT 1 cut(s) 837
Bst6I CTCTTC 2 cut(s) 295, 364
BstAFI CTTAAG 1 cut(s) 706
BstBI TTCGAA 1 cut(s) 958
BstC8I GCNNGC 3 cut(s) 536, 733, 983
BstDEI CTNAG 1 cut(s) 429
BstDSI CCRYGG 1 cut(s) 1006
BstF5I GGATG 7 cut(s) 81, 136, 425, 516, 733, 757, 1025
BstFNI CGCG 1 cut(s) 322
BstHHI GCGC 1 cut(s) 324
BstKTI GATC 6 cut(s) 395, 526, 607, 995, 1088, 1118
BstMAI GTCTC 2 cut(s) 659, 695
BstMBI GATC 6 cut(s) 392, 523, 604, 992, 1085, 1115
BstMWI GCNNNNNNNGC 3 cut(s) 987, 996, 1050
BstNI CCWGG 2 cut(s) 506, 749
BstNSI RCATGY 3 cut(s) 275, 474, 985
BstSCI CCNGG 2 cut(s) 504, 747
BstSFI CTRYAG 1 cut(s) 909
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 1 cut(s) 400
BstV2I GAAGAC 2 cut(s) 103, 606
BstX2I RGATCY 1 cut(s) 992
BstYI RGATCY 1 cut(s) 992
BsuRI GGCC 4 cut(s) 198, 222, 1005, 1041
BtgI CCRYGG 1 cut(s) 1006
BtsCI GGATG 7 cut(s) 81, 136, 425, 516, 733, 757, 1025
BtsIMutI CAGTG 2 cut(s) 624, 1103
Cac8I GCNNGC 3 cut(s) 536, 733, 983
CfoI GCGC 1 cut(s) 324
Cfr13I GGNCC 4 cut(s) 196, 257, 915, 1125
Csp6I GTAC 2 cut(s) 181, 202
CviAII CATG 6 cut(s) 16, 272, 471, 488, 982, 1089
CviQI GTAC 2 cut(s) 181, 202
DdeI CTNAG 1 cut(s) 429
DpnI GATC 6 cut(s) 394, 525, 606, 994, 1087, 1117
DpnII GATC 6 cut(s) 392, 523, 604, 992, 1085, 1115
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
EaeI YGGCCR 2 cut(s) 220, 1039
Eam1104I CTCTTC 2 cut(s) 295, 364
EarI CTCTTC 2 cut(s) 295, 364
Eco130I CCWWGG 2 cut(s) 126, 1000
Eco31I GGTCTC 1 cut(s) 695
Eco32I GATATC 1 cut(s) 592
Eco47I GGWCC 3 cut(s) 257, 915, 1125
Eco57I CTGAAG 1 cut(s) 942
Eco88I CYCGRG 1 cut(s) 1073
EcoRII CCWGG 2 cut(s) 504, 747
EcoRV GATATC 1 cut(s) 592
EcoT14I CCWWGG 2 cut(s) 126, 1000
ErhI CCWWGG 2 cut(s) 126, 1000
Esp3I CGTCTC 1 cut(s) 659
FaeI CATG 6 cut(s) 19, 275, 474, 491, 985, 1092
FalI AAGNNNNNCTT 2 cut(s) 915, 947
FaqI GGGAC 3 cut(s) 107, 329, 731
FatI CATG 6 cut(s) 15, 271, 470, 487, 981, 1088
FauI CCCGC 2 cut(s) 409, 1136
FbaI TGATCA 1 cut(s) 523
Fnu4HI GCNGC 2 cut(s) 414, 1042
FokI GGATG 7 cut(s) 68, 143, 432, 503, 740, 764, 1032
Fsp4HI GCNGC 2 cut(s) 414, 1042
FspBI CTAG 2 cut(s) 611, 1156
GlaI GCGC 1 cut(s) 323
GluI GCNGC 2 cut(s) 414, 1042
GsaI CCCAGC 1 cut(s) 990
GsuI CTGGAG 2 cut(s) 386, 497
HaeIII GGCC 4 cut(s) 198, 222, 1005, 1041
HapII CCGG 1 cut(s) 188
HhaI GCGC 1 cut(s) 324
Hin1II CATG 6 cut(s) 19, 275, 474, 491, 985, 1092
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 3 cut(s) 304, 482, 614
HpaII CCGG 1 cut(s) 188
HphI GGTGA 3 cut(s) 202, 1043, 1110
Hpy166II GTNNAC 3 cut(s) 181, 511, 941
Hpy188I TCNGA 5 cut(s) 295, 389, 432, 1063, 1125
Hpy188III TCNNGA 6 cut(s) 47, 365, 476, 611, 822, 1073
Hpy8I GTNNAC 3 cut(s) 181, 511, 941
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 4 cut(s) 64, 169, 640, 718
HpyCH4III ACNGT 1 cut(s) 837
HpyCH4IV ACGT 1 cut(s) 627
HpyCH4V TGCA 5 cut(s) 216, 248, 280, 731, 1112
HpyF10VI GCNNNNNNNGC 3 cut(s) 987, 996, 1050
HpyF3I CTNAG 1 cut(s) 429
HpySE526I ACGT 1 cut(s) 627
Hsp92II CATG 6 cut(s) 19, 275, 474, 491, 985, 1092
HspAI GCGC 1 cut(s) 322
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 6 cut(s) 392, 523, 604, 992, 1085, 1115
LguI GCTCTTC 2 cut(s) 295, 364
LmnI GCTCC 2 cut(s) 453, 1075
Lsp1109I GCAGC 1 cut(s) 400
LweI GCATC 4 cut(s) 415, 718, 742, 1153
MaeI CTAG 2 cut(s) 611, 1156
MaeII ACGT 1 cut(s) 627
MaeIII GTNAC 3 cut(s) 190, 499, 875
MalI GATC 6 cut(s) 394, 525, 606, 994, 1087, 1117
MboI GATC 6 cut(s) 392, 523, 604, 992, 1085, 1115
MboII GAAGA 7 cut(s) 103, 282, 351, 398, 506, 611, 1046
MfeI CAATTG 1 cut(s) 639
MflI RGATCY 1 cut(s) 992
MhlI GDGCHC 1 cut(s) 1103
MlsI TGGCCA 1 cut(s) 222
MluCI AATT 5 cut(s) 76, 117, 243, 639, 846
MluNI TGGCCA 1 cut(s) 222
MnlI CCTC 8 cut(s) 13, 19, 45, 383, 444, 472, 929, 1131
Mox20I TGGCCA 1 cut(s) 222
MroXI GAANNNNTTC 1 cut(s) 927
MscI TGGCCA 1 cut(s) 222
MseI TTAA 1 cut(s) 707
MslI CAYNNNNRTG 1 cut(s) 20
Msp20I TGGCCA 1 cut(s) 222
MspA1I CMGCKG 2 cut(s) 920, 990
MspCI CTTAAG 1 cut(s) 706
MspI CCGG 1 cut(s) 188
MspR9I CCNGG 2 cut(s) 506, 749
MunI CAATTG 1 cut(s) 639
Mva1269I GAATGC 1 cut(s) 248
MvaI CCWGG 2 cut(s) 506, 749
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 3 cut(s) 987, 996, 1050
NdeII GATC 6 cut(s) 392, 523, 604, 992, 1085, 1115
NlaIII CATG 6 cut(s) 19, 275, 474, 491, 985, 1092
NlaIV GGNNCC 3 cut(s) 916, 994, 1127
NmuCI GTSAC 1 cut(s) 190
NspI RCATGY 3 cut(s) 275, 474, 985
NspV TTCGAA 1 cut(s) 958
PaeI GCATGC 1 cut(s) 985
PciI ACATGT 1 cut(s) 271
PciSI GCTCTTC 2 cut(s) 295, 364
PcsI WCGNNNNNNNCGW 1 cut(s) 792
PctI GAATGC 1 cut(s) 248
PdmI GAANNNNTTC 1 cut(s) 927
PfeI GAWTC 3 cut(s) 304, 482, 614
PflFI GACNNNGTC 1 cut(s) 107
PfoI TCCNGGA 1 cut(s) 747
PkrI GCNGC 2 cut(s) 415, 1043
PscI ACATGT 1 cut(s) 271
PsiI TTATAA 1 cut(s) 894
Psp1406I AACGTT 1 cut(s) 627
Psp6I CCWGG 2 cut(s) 504, 747
PspFI CCCAGC 1 cut(s) 986
PspGI CCWGG 2 cut(s) 504, 747
PspN4I GGNNCC 3 cut(s) 916, 994, 1127
PspPI GGNCC 4 cut(s) 196, 257, 915, 1125
PsuI RGATCY 1 cut(s) 992
PsyI GACNNNGTC 1 cut(s) 107
RsaI GTAC 2 cut(s) 182, 203
RsaNI GTAC 2 cut(s) 181, 202
RseI CAYNNNNRTG 1 cut(s) 20
SapI GCTCTTC 2 cut(s) 295, 364
SaqAI TTAA 1 cut(s) 707
SatI GCNGC 2 cut(s) 414, 1042
Sau3AI GATC 6 cut(s) 392, 523, 604, 992, 1085, 1115
Sau96I GGNCC 4 cut(s) 196, 257, 915, 1125
ScaI AGTACT 1 cut(s) 203
ScrFI CCNGG 2 cut(s) 506, 749
SduI GDGCHC 1 cut(s) 1103
SfaNI GCATC 4 cut(s) 415, 718, 742, 1153
SfcI CTRYAG 1 cut(s) 909
SfuI TTCGAA 1 cut(s) 958
SinI GGWCC 3 cut(s) 257, 915, 1125
SmiMI CAYNNNNRTG 1 cut(s) 20
SmlI CTYRAG 2 cut(s) 706, 820
SmoI CTYRAG 2 cut(s) 706, 820
SphI GCATGC 1 cut(s) 985
Sse9I AATT 5 cut(s) 76, 117, 243, 639, 846
SsiI CCGC 6 cut(s) 149, 416, 918, 990, 1042, 1129
SspMI CTAG 2 cut(s) 611, 1156
StyD4I CCNGG 2 cut(s) 504, 747
StyI CCWWGG 2 cut(s) 126, 1000
TaaI ACNGT 1 cut(s) 837
TaiI ACGT 1 cut(s) 630
TaqI TCGA 6 cut(s) 161, 307, 786, 795, 958, 1097
TasI AATT 5 cut(s) 76, 117, 243, 639, 846
TatI WGTACW 2 cut(s) 180, 201
TauI GCSGC 1 cut(s) 1044
TfiI GAWTC 3 cut(s) 304, 482, 614
Tru1I TTAA 1 cut(s) 707
Tru9I TTAA 1 cut(s) 707
TscAI CASTG 2 cut(s) 624, 1110
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 1 cut(s) 413
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 5 cut(s) 330, 338, 505, 612, 687
TspGWI ACGGA 3 cut(s) 26, 27, 222
TspRI CASTG 2 cut(s) 624, 1110
Tth111I GACNNNGTC 1 cut(s) 107
Vha464I CTTAAG 1 cut(s) 706
VpaK11BI GGWCC 3 cut(s) 257, 915, 1125
XapI RAATTY 1 cut(s) 76
XbaI TCTAGA 1 cut(s) 610
XceI RCATGY 3 cut(s) 275, 474, 985
XcmI CCANNNNNNNNNTGG 1 cut(s) 1013
XmnI GAANNNNTTC 1 cut(s) 927
XspI CTAG 2 cut(s) 611, 1156
ZrmI AGTACT 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.