Rh6CG364900

WD repeat-containing protein 89 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0009953
Physical Location & Seq
Reverse (-)
55263037 .. 55265129
2093 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG364900.1

Sequence Viewer

Length: 807 bp
ATGGAAGCCATGGACTTGGATATGGAATCGAAACCTCAACCGCAGGAGTCTCACTCCAATCCAAACTCATCCAAGCGTTTCGGCCTCAAAACCTCTATCCCAACCAACTTTGGAGACGATTACGTTTTCCAAATTGTGCCCAAGGATGATTGGACAGCAATGGCGGTGTCACTGTCAACCAACGCAGTGAAGCTCTACTCACCGGTGACTGGTCAGTACTACGGAGAGTGCAAAGGCCACTCTGCAACTATCAATCAAATTTCATTTGCAGACCCATCATCCCCACATGTTTTGCACTCCTGCTCTTCCGACGGAACCATTAGACTTTGGGACTCCCGCACGTTTCAACAGGTGTCATCTTTTCACTCTGGTTCGAGTGAGGAGATTTTCAGCTTTTCGTTTGGTGGATCATCTAATAATCTTCTGGCCGCAGGATGTAATTCTCAGATACTAATGTGGGATTGGAGGAACGACAAACAAGTGGCATGCTTAGAGGACTCTCATGTGGAAGATGTTACTCAGGTTCACTTCATTCCTGACCATCAAAGCAAGCTCCTTTCTGCTTCCGTAGATGGATTGATATGTATATTTGATACAGATGGAGATATCAACGAAGATGATCATCTTGAGTCAGTGCTAAATGTGGGCACTTCCATTGGGAAGGTGGGGTTTTTTGGTGAGACGTATCAAAAGCTTTGGTGTTTGACCCATATTGAAACCTTAAGGTTGATTATTTTGTTGATTGCCACTACTCAAAAGAAGCAGAACGATTATGGCTTATTGGTGGCGCCAACACTGGCACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010029 GO:0040008 GO:0048580 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:1900140 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

268

Amino Acids

29.62

Weight (kDa)

4.82

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 39 - 200 1.8e-12 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 42 - 199 2.2e-10 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 52 - 200 1e-23 WDR5 beta-propeller domain
WDR55 PF24796 53 - 200 2e-09 WDR55
Beta-prop_WDR75_1st PF23869 57 - 112 3.3e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR3_1st PF25173 58 - 200 6.2e-17 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 60 - 160 7.4e-12 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 61 - 155 3.6e-08 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 62 - 182 5e-12 Prp19 WD40 domain
WD40 PF00400 74 - 111 2.4e-06 WD domain, G-beta repeat
WD40_Gbeta PF25391 78 - 202 2e-07 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 92 - 205 4.1e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 98 - 200 1.7e-06 WDR90/POC16, second beta-propeller
Beta-prop_EML PF23409 102 - 187 4.9e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EIPR1 PF23609 113 - 199 1.5e-06 EIPR1 beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 117 - 195 7.7e-06 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 121 - 202 8.4e-06 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 129 - 200 5e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 787
AciI CCGC 4 cut(s) 41, 164, 337, 429
AclWI GGATC 1 cut(s) 415
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 1 cut(s) 258
AcyI GRCGYC 1 cut(s) 788
AfaI GTAC 1 cut(s) 218
AfiI CCNNNNNNNGG 1 cut(s) 209
AflII CTTAAG 1 cut(s) 721
AflIII ACRYGT 1 cut(s) 286
AgeI ACCGGT 1 cut(s) 202
AgsI TTSAA 2 cut(s) 347, 716
AluBI AGCT 4 cut(s) 193, 393, 553, 694
AluI AGCT 4 cut(s) 193, 393, 553, 694
Alw26I GTCTC 3 cut(s) 54, 108, 674
AlwI GGATC 1 cut(s) 415
AoxI GGCC 3 cut(s) 82, 235, 426
ApoI RAATTY 1 cut(s) 258
AsiGI ACCGGT 1 cut(s) 202
AspLEI GCGC 1 cut(s) 790
AsuHPI GGTGA 3 cut(s) 192, 217, 689
BaeGI GKGCMC 2 cut(s) 141, 650
BaeI ACNNNNGTAYC 2 cut(s) 440, 473
BanI GGYRCC 1 cut(s) 787
BccI CCATC 4 cut(s) 283, 549, 566, 593
BclI TGATCA 1 cut(s) 619
BcoDI GTCTC 3 cut(s) 54, 108, 674
BfoI RGCGCY 1 cut(s) 791
BfrI CTTAAG 1 cut(s) 721
BisI GCNGC 1 cut(s) 429
BlsI GCNGC 1 cut(s) 430
BmcAI AGTACT 1 cut(s) 218
BmiI GGNNCC 2 cut(s) 316, 789
BplI GAGNNNNNCTC 2 cut(s) 38, 70
BpuEI CTTGAG 1 cut(s) 647
BsaBI GATNNNNATC 1 cut(s) 621
BsaHI GRCGYC 1 cut(s) 788
BsaJI CCNNGG 2 cut(s) 9, 141
BsaWI WCCGGW 1 cut(s) 202
Bsc4I CCNNNNNNNGG 1 cut(s) 209
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 2 cut(s) 214, 801
Bse3DI GCAATG 1 cut(s) 165
Bse8I GATNNNNATC 1 cut(s) 621
BseDI CCNNGG 2 cut(s) 9, 141
BseGI GGATG 4 cut(s) 68, 151, 278, 440
BseJI GATNNNNATC 1 cut(s) 621
BseLI CCNNNNNNNGG 1 cut(s) 209
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 2 cut(s) 458, 533
BseNI ACTGG 2 cut(s) 214, 801
BseRI GAGGAG 1 cut(s) 395
BseSI GKGCMC 2 cut(s) 141, 650
BshFI GGCC 3 cut(s) 84, 237, 428
BshNI GGYRCC 1 cut(s) 787
BshTI ACCGGT 1 cut(s) 202
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 1 cut(s) 209
BsmAI GTCTC 3 cut(s) 54, 108, 674
BsmBI CGTCTC 2 cut(s) 108, 674
BsmFI GGGAC 1 cut(s) 344
BsnI GGCC 3 cut(s) 84, 237, 428
Bsp1286I GDGCHC 2 cut(s) 141, 650
Bsp143I GATC 2 cut(s) 407, 619
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 4 cut(s) 41, 164, 337, 429
BspANI GGCC 3 cut(s) 84, 237, 428
BspCNI CTCAG 2 cut(s) 457, 532
BspLI GGNNCC 2 cut(s) 316, 789
BspPI GGATC 1 cut(s) 415
BspQI GCTCTTC 1 cut(s) 310
BspT107I GGYRCC 1 cut(s) 787
BspTI CTTAAG 1 cut(s) 721
BsrDI GCAATG 1 cut(s) 165
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 2 cut(s) 214, 801
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 2 cut(s) 9, 141
BssMI GATC 2 cut(s) 407, 619
BssNI GRCGYC 1 cut(s) 788
BssT1I CCWWGG 2 cut(s) 9, 141
Bst4CI ACNGT 1 cut(s) 174
Bst6I CTCTTC 1 cut(s) 310
BstACI GRCGYC 1 cut(s) 788
BstAFI CTTAAG 1 cut(s) 721
BstC8I GCNNGC 2 cut(s) 487, 551
BstDEI CTNAG 3 cut(s) 444, 490, 519
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 4 cut(s) 68, 151, 278, 440
BstH2I RGCGCY 1 cut(s) 791
BstHHI GCGC 1 cut(s) 790
BstKTI GATC 2 cut(s) 410, 622
BstMAI GTCTC 3 cut(s) 54, 108, 674
BstMBI GATC 2 cut(s) 407, 619
BstNSI RCATGY 2 cut(s) 290, 489
BstSLI GKGCMC 2 cut(s) 141, 650
BstXI CCANNNNNNTGG 1 cut(s) 16
BsuRI GGCC 3 cut(s) 84, 237, 428
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 4 cut(s) 68, 151, 278, 440
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 4 cut(s) 170, 192, 639, 794
Cac8I GCNNGC 2 cut(s) 487, 551
CfoI GCGC 1 cut(s) 790
Cfr10I RCCGGY 1 cut(s) 202
Csp6I GTAC 1 cut(s) 217
CspAI ACCGGT 1 cut(s) 202
CspCI CAANNNNNGTGG 2 cut(s) 273, 308
CviAII CATG 4 cut(s) 10, 287, 486, 503
CviJI RGCY 9 cut(s) 8, 84, 193, 237, 393, 428, 553, 694, 777
CviKI_1 RGCY 9 cut(s) 8, 84, 193, 237, 393, 428, 553, 694, 777
CviQI GTAC 1 cut(s) 217
DdeI CTNAG 3 cut(s) 444, 490, 519
DinI GGCGCC 1 cut(s) 789
DpnI GATC 2 cut(s) 409, 621
DpnII GATC 2 cut(s) 407, 619
EaeI YGGCCR 1 cut(s) 426
Eam1104I CTCTTC 1 cut(s) 310
EarI CTCTTC 1 cut(s) 310
Eco130I CCWWGG 2 cut(s) 9, 141
Eco32I GATATC 1 cut(s) 607
EcoRV GATATC 1 cut(s) 607
EcoT14I CCWWGG 2 cut(s) 9, 141
EgeI GGCGCC 1 cut(s) 789
EheI GGCGCC 1 cut(s) 789
ErhI CCWWGG 2 cut(s) 9, 141
Esp3I CGTCTC 2 cut(s) 108, 674
FaeI CATG 4 cut(s) 13, 290, 489, 506
FaiI YATR 9 cut(s) 11, 23, 288, 487, 504, 583, 587, 711, 774
FaqI GGGAC 1 cut(s) 344
FatI CATG 4 cut(s) 9, 286, 485, 502
FauI CCCGC 1 cut(s) 344
FbaI TGATCA 1 cut(s) 619
Fnu4HI GCNGC 1 cut(s) 429
FokI GGATG 4 cut(s) 55, 158, 265, 447
Fsp4HI GCNGC 1 cut(s) 429
GlaI GCGC 1 cut(s) 789
GluI GCNGC 1 cut(s) 429
HaeII RGCGCY 1 cut(s) 791
HaeIII GGCC 3 cut(s) 84, 237, 428
HapII CCGG 1 cut(s) 203
HhaI GCGC 1 cut(s) 790
Hin1I GRCGYC 1 cut(s) 788
Hin1II CATG 4 cut(s) 13, 290, 489, 506
Hin6I GCGC 1 cut(s) 788
HinP1I GCGC 1 cut(s) 788
HincII GTYRAC 1 cut(s) 177
HindII GTYRAC 1 cut(s) 177
HindIII AAGCTT 1 cut(s) 692
HinfI GANTC 5 cut(s) 26, 47, 332, 497, 629
HpaII CCGG 1 cut(s) 203
HphI GGTGA 3 cut(s) 192, 217, 689
Hpy166II GTNNAC 2 cut(s) 177, 526
Hpy188I TCNGA 2 cut(s) 310, 447
Hpy188III TCNNGA 2 cut(s) 536, 626
Hpy8I GTNNAC 2 cut(s) 177, 526
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 1 cut(s) 655
HpyCH4III ACNGT 1 cut(s) 174
HpyCH4IV ACGT 3 cut(s) 123, 341, 683
HpyCH4V TGCA 4 cut(s) 231, 245, 269, 295
HpyF3I CTNAG 3 cut(s) 444, 490, 519
HpySE526I ACGT 3 cut(s) 123, 341, 683
Hsp92I GRCGYC 1 cut(s) 788
Hsp92II CATG 4 cut(s) 13, 290, 489, 506
HspAI GCGC 1 cut(s) 788
KasI GGCGCC 1 cut(s) 787
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 2 cut(s) 407, 619
LguI GCTCTTC 1 cut(s) 310
LmnI GCTCC 1 cut(s) 558
MaeII ACGT 3 cut(s) 123, 341, 683
MaeIII GTNAC 3 cut(s) 168, 205, 514
MalI GATC 2 cut(s) 409, 621
MboI GATC 2 cut(s) 407, 619
MboII GAAGA 4 cut(s) 297, 413, 521, 626
MhlI GDGCHC 2 cut(s) 141, 650
MluCI AATT 3 cut(s) 132, 258, 439
Mly113I GGCGCC 1 cut(s) 788
MlyI GAGTC 4 cut(s) 56, 326, 491, 638
MmeI TCCRAC 1 cut(s) 333
MnlI CCTC 6 cut(s) 45, 95, 103, 373, 459, 487
MseI TTAA 1 cut(s) 722
MspCI CTTAAG 1 cut(s) 721
MspI CCGG 1 cut(s) 203
NarI GGCGCC 1 cut(s) 788
NcoI CCATGG 1 cut(s) 9
NdeII GATC 2 cut(s) 407, 619
NlaIII CATG 4 cut(s) 13, 290, 489, 506
NlaIV GGNNCC 2 cut(s) 316, 789
NmuCI GTSAC 2 cut(s) 168, 205
NspI RCATGY 2 cut(s) 290, 489
PaeI GCATGC 1 cut(s) 489
PciI ACATGT 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 310
PfeI GAWTC 1 cut(s) 26
PinAI ACCGGT 1 cut(s) 202
PkrI GCNGC 1 cut(s) 430
PleI GAGTC 4 cut(s) 55, 326, 491, 637
PluTI GGCGCC 1 cut(s) 791
PpsI GAGTC 4 cut(s) 55, 326, 491, 637
PscI ACATGT 1 cut(s) 286
PspN4I GGNNCC 2 cut(s) 316, 789
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
SapI GCTCTTC 1 cut(s) 310
SaqAI TTAA 1 cut(s) 722
SatI GCNGC 1 cut(s) 429
Sau3AI GATC 2 cut(s) 407, 619
ScaI AGTACT 1 cut(s) 218
SchI GAGTC 4 cut(s) 56, 326, 491, 638
SduI GDGCHC 2 cut(s) 141, 650
SfoI GGCGCC 1 cut(s) 789
SgrAI CRCCGGYG 1 cut(s) 202
SmlI CTYRAG 2 cut(s) 626, 721
SmoI CTYRAG 2 cut(s) 626, 721
SphI GCATGC 1 cut(s) 489
Sse9I AATT 3 cut(s) 132, 258, 439
SsiI CCGC 4 cut(s) 41, 164, 337, 429
SspDI GGCGCC 1 cut(s) 787
StyI CCWWGG 2 cut(s) 9, 141
TaaI ACNGT 1 cut(s) 174
TaiI ACGT 3 cut(s) 126, 344, 686
TaqI TCGA 2 cut(s) 29, 374
TasI AATT 3 cut(s) 132, 258, 439
TatI WGTACW 1 cut(s) 216
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 1 cut(s) 722
Tru9I TTAA 1 cut(s) 722
TscAI CASTG 4 cut(s) 177, 192, 639, 801
TseFI GTSAC 2 cut(s) 168, 205
Tsp45I GTSAC 2 cut(s) 168, 205
TspDTI ATGAA 2 cut(s) 252, 520
TspGWI ACGGA 3 cut(s) 237, 327, 556
TspRI CASTG 4 cut(s) 177, 192, 639, 801
Vha464I CTTAAG 1 cut(s) 721
XapI RAATTY 1 cut(s) 258
XceI RCATGY 2 cut(s) 290, 489
XcmI CCANNNNNNNNNTGG 1 cut(s) 661
ZrmI AGTACT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.