Rroxscaffold_7G00173930

WD repeat-containing protein 89 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009953
Physical Location & Seq
Forward (+)
13787124 .. 13789718
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00173930.1

Sequence Viewer

Length: 1173 bp
ATGGAAGCCATGGACTTGGATGTGGAGGCAAAACCTCAATCCGAGTCTCAGTCCAATCCAAACTCATTCAAGCGTTTCGGCCTCAAAACCTCTATCCCAACCAACTTCGGAGACGATTACGTTTTCCAAATTGTGCCCAAGGATGATTGGACAGCAATGGCGGTGTCACTGTCAACGAACGCAGTGAAGCTCTACTCACCGGTGACTGGTCAGTACTATGGAGAGTGCAAAGGCCACTCTGCAACTATCAATCAAATTTCATTTGCAGACCCATTATCCCCACATGTTCTGCACTCCTGCTCTTCCGACGGAACCATTAGACTTTGGGACTCCCGCACATTCCAACAGGTGTCATCTTTTCACTCTGGTTCGAGTGAGGAGATTTTCAGCTTTTCGTTTGGTGGATCATCTAATAATCTTCTGGCCGCAGGATGTAATTCTCAGATACTAATGTGGGATTGGAGGAACGACAAACAAGTAGCATGCTTAGAGGACTCTCATGTGGAAGATGTTACTCAGGCAGGCTCTTATGCATTTGCGATCATGGAATTCAAGCTCCTTTCTGCTTCTGTAGATGGATTGATATGTATATTTGATACAGATGGAGATATCAACGAAGATGATCATCTTGAGTCAGTGCTAAATGTGGGCACTTCCATTGGGAAGGTGGGGTTTTTTGGTGAGACGTATCAAAAGCTTTGGTGTTTGACCCATATTGAAACTCTAAGTATCTGGGACTGGAAGGATGCAACTGAAACAAATTTCCAGGACGCTCGTTCTTTGGCTTCTAACAGTTGGACTCTAGGTGAAGTTGATTATTTTGTTGATTGCCACTACTCAAGAGAAGCAGAACGATTATGGCTTATTGGTGGCGCTAACACTGGCACTTTAGGTTACTTCCCTGTAACTTACACAGGAACGCGAGCCATAGGTTCCCCTGAAGCAATTATGGAAGGCGGCCATACGGGTGTTGTCCGAAGTGTGTTGCCTATGTCAAATATGCCAGGTCCCCAAGGCCAAAGCATTTTTGGATGGACTGGTGGTGAAGATGGCCATTTATGTTGCTGGTTATCGGACGGTTCTCTGGACACCATAAACCGATCATGGAGTTCAAGCGAACTGGTTTTGAGATCACCAAAGATCCGAAAGTCAAAAAGGCATAGTCCCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010029 GO:0040008 GO:0048580 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:1900140 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

390

Amino Acids

43.02

Weight (kDa)

4.8

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 38 - 164 3.7e-09 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 41 - 201 4.3e-09 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 51 - 201 6.4e-20 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 55 - 201 2.2e-12 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 60 - 161 1.6e-11 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 60 - 154 1.6e-07 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 61 - 167 6.3e-09 Prp19 WD40 domain
WDR55 PF24796 72 - 203 4.9e-07 WDR55
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 922
AciI CCGC 4 cut(s) 161, 334, 426, 957
AclWI GGATC 2 cut(s) 412, 1135
AcoI YGGCCR 3 cut(s) 423, 958, 1051
AcsI RAATTY 3 cut(s) 255, 548, 760
AcuI CTGAAG 1 cut(s) 960
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 1 cut(s) 206
AflIII ACRYGT 1 cut(s) 283
AgeI ACCGGT 1 cut(s) 199
AgsI TTSAA 4 cut(s) 70, 553, 719, 1113
AjnI CCWGG 2 cut(s) 765, 1003
AluBI AGCT 4 cut(s) 190, 390, 556, 697
AluI AGCT 4 cut(s) 190, 390, 556, 697
Alw26I GTCTC 3 cut(s) 51, 105, 677
AlwI GGATC 2 cut(s) 412, 1135
AoxI GGCC 6 cut(s) 79, 232, 423, 958, 1015, 1051
ApoI RAATTY 3 cut(s) 255, 548, 760
AsiGI ACCGGT 1 cut(s) 199
AspLEI GCGC 1 cut(s) 875
AspS9I GGNCC 1 cut(s) 1007
AsuHPI GGTGA 6 cut(s) 189, 214, 692, 818, 1055, 1125
AvaII GGWCC 1 cut(s) 1007
BaeGI GKGCMC 2 cut(s) 138, 653
BaeI ACNNNNGTAYC 2 cut(s) 437, 470
BalI TGGCCA 1 cut(s) 1053
BccI CCATC 4 cut(s) 569, 596, 1026, 1043
BciT130I CCWGG 2 cut(s) 767, 1005
BclI TGATCA 1 cut(s) 622
BcoDI GTCTC 3 cut(s) 51, 105, 677
BfaI CTAG 1 cut(s) 803
BfmI CTRYAG 1 cut(s) 570
BfoI RGCGCY 1 cut(s) 876
BisI GCNGC 2 cut(s) 426, 958
BlsI GCNGC 2 cut(s) 427, 959
BmcAI AGTACT 1 cut(s) 215
Bme1390I CCNGG 2 cut(s) 767, 1005
Bme18I GGWCC 1 cut(s) 1007
BmgT120I GGNCC 1 cut(s) 1007
BmiI GGNNCC 3 cut(s) 313, 934, 1009
BmrFI CCNGG 2 cut(s) 767, 1005
BmsI GCATC 1 cut(s) 736
BpuEI CTTGAG 2 cut(s) 650, 823
BsaBI GATNNNNATC 1 cut(s) 624
BsaJI CCNNGG 3 cut(s) 9, 138, 1012
BsaWI WCCGGW 1 cut(s) 199
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse118I RCCGGY 1 cut(s) 199
Bse1I ACTGG 5 cut(s) 211, 743, 886, 1042, 1125
Bse3DI GCAATG 1 cut(s) 162
Bse8I GATNNNNATC 1 cut(s) 624
BseBI CCWGG 2 cut(s) 767, 1005
BseDI CCNNGG 3 cut(s) 9, 138, 1012
BseGI GGATG 5 cut(s) 25, 148, 437, 751, 1037
BseJI GATNNNNATC 1 cut(s) 624
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 3 cut(s) 62, 455, 530
BseNI ACTGG 5 cut(s) 211, 743, 886, 1042, 1125
BseRI GAGGAG 1 cut(s) 392
BseSI GKGCMC 2 cut(s) 138, 653
BsgI GTGCAG 1 cut(s) 275
Bsh1236I CGCG 1 cut(s) 922
BshFI GGCC 6 cut(s) 81, 234, 425, 960, 1017, 1053
BshTI ACCGGT 1 cut(s) 199
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 4 cut(s) 341, 749, 993, 1149
BslI CCNNNNNNNGG 1 cut(s) 206
BsmAI GTCTC 3 cut(s) 51, 105, 677
BsmBI CGTCTC 2 cut(s) 105, 677
BsmFI GGGAC 4 cut(s) 341, 749, 993, 1149
BsnI GGCC 6 cut(s) 81, 234, 425, 960, 1017, 1053
Bsp1286I GDGCHC 2 cut(s) 138, 653
Bsp143I GATC 6 cut(s) 404, 540, 622, 1100, 1130, 1140
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 4 cut(s) 161, 334, 426, 957
BspANI GGCC 6 cut(s) 81, 234, 425, 960, 1017, 1053
BspCNI CTCAG 3 cut(s) 61, 454, 529
BspFNI CGCG 1 cut(s) 922
BspLI GGNNCC 3 cut(s) 313, 934, 1009
BspPI GGATC 2 cut(s) 412, 1135
BspQI GCTCTTC 1 cut(s) 307
BsrDI GCAATG 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 199
BsrI ACTGG 5 cut(s) 211, 743, 886, 1042, 1125
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 3 cut(s) 9, 138, 1012
BssMI GATC 6 cut(s) 404, 540, 622, 1100, 1130, 1140
BssT1I CCWWGG 3 cut(s) 9, 138, 1012
Bst2UI CCWGG 2 cut(s) 767, 1005
Bst4CI ACNGT 3 cut(s) 171, 794, 1079
Bst6I CTCTTC 1 cut(s) 307
BstC8I GCNNGC 3 cut(s) 484, 523, 924
BstDEI CTNAG 5 cut(s) 48, 441, 487, 516, 725
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 5 cut(s) 25, 148, 437, 751, 1037
BstFNI CGCG 1 cut(s) 922
BstH2I RGCGCY 1 cut(s) 876
BstHHI GCGC 1 cut(s) 875
BstKTI GATC 6 cut(s) 407, 543, 625, 1103, 1133, 1143
BstMAI GTCTC 3 cut(s) 51, 105, 677
BstMBI GATC 6 cut(s) 404, 540, 622, 1100, 1130, 1140
BstNI CCWGG 2 cut(s) 767, 1005
BstNSI RCATGY 2 cut(s) 287, 486
BstSCI CCNGG 2 cut(s) 765, 1003
BstSFI CTRYAG 1 cut(s) 570
BstSLI GKGCMC 2 cut(s) 138, 653
BstUI CGCG 1 cut(s) 922
BstX2I RGATCY 1 cut(s) 1140
BstXI CCANNNNNNTGG 1 cut(s) 16
BstYI RGATCY 1 cut(s) 1140
BsuRI GGCC 6 cut(s) 81, 234, 425, 960, 1017, 1053
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 5 cut(s) 25, 148, 437, 751, 1037
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 4 cut(s) 167, 189, 642, 879
Cac8I GCNNGC 3 cut(s) 484, 523, 924
CfoI GCGC 1 cut(s) 875
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 1007
CseI GACGC 1 cut(s) 779
Csp6I GTAC 1 cut(s) 214
CspAI ACCGGT 1 cut(s) 199
CviAII CATG 6 cut(s) 10, 284, 483, 500, 544, 1104
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 5 cut(s) 48, 441, 487, 516, 725
DpnI GATC 6 cut(s) 406, 542, 624, 1102, 1132, 1142
DpnII GATC 6 cut(s) 404, 540, 622, 1100, 1130, 1140
EaeI YGGCCR 3 cut(s) 423, 958, 1051
Eam1104I CTCTTC 1 cut(s) 307
EarI CTCTTC 1 cut(s) 307
Eco130I CCWWGG 3 cut(s) 9, 138, 1012
Eco32I GATATC 1 cut(s) 610
Eco47I GGWCC 1 cut(s) 1007
Eco57I CTGAAG 1 cut(s) 960
EcoO109I RGGNCCY 1 cut(s) 1007
EcoRI GAATTC 1 cut(s) 548
EcoRII CCWGG 2 cut(s) 765, 1003
EcoRV GATATC 1 cut(s) 610
EcoT14I CCWWGG 3 cut(s) 9, 138, 1012
EcoT22I ATGCAT 1 cut(s) 535
ErhI CCWWGG 3 cut(s) 9, 138, 1012
Esp3I CGTCTC 2 cut(s) 105, 677
FaeI CATG 6 cut(s) 13, 287, 486, 503, 547, 1107
FaqI GGGAC 4 cut(s) 341, 749, 993, 1149
FatI CATG 6 cut(s) 9, 283, 482, 499, 543, 1103
FauI CCCGC 1 cut(s) 341
FbaI TGATCA 1 cut(s) 622
Fnu4HI GCNGC 2 cut(s) 426, 958
FokI GGATG 5 cut(s) 32, 155, 444, 758, 1044
Fsp4HI GCNGC 2 cut(s) 426, 958
FspBI CTAG 1 cut(s) 803
GlaI GCGC 1 cut(s) 874
GluI GCNGC 2 cut(s) 426, 958
HaeII RGCGCY 1 cut(s) 876
HaeIII GGCC 6 cut(s) 81, 234, 425, 960, 1017, 1053
HapII CCGG 1 cut(s) 200
HgaI GACGC 1 cut(s) 779
HhaI GCGC 1 cut(s) 875
Hin1II CATG 6 cut(s) 13, 287, 486, 503, 547, 1107
Hin6I GCGC 1 cut(s) 873
HinP1I GCGC 1 cut(s) 873
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HindIII AAGCTT 1 cut(s) 695
HinfI GANTC 5 cut(s) 44, 329, 494, 632, 799
HpaII CCGG 1 cut(s) 200
HphI GGTGA 6 cut(s) 189, 214, 692, 818, 1055, 1125
Hpy166II GTNNAC 1 cut(s) 174
Hpy188I TCNGA 7 cut(s) 43, 110, 307, 444, 977, 1075, 1145
Hpy188III TCNNGA 3 cut(s) 629, 840, 1085
Hpy8I GTNNAC 1 cut(s) 174
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 3 cut(s) 658, 736, 947
HpyCH4III ACNGT 3 cut(s) 171, 794, 1079
HpyCH4IV ACGT 2 cut(s) 120, 686
HpyCH4V TGCA 6 cut(s) 228, 242, 266, 292, 533, 749
HpyF3I CTNAG 5 cut(s) 48, 441, 487, 516, 725
HpySE526I ACGT 2 cut(s) 120, 686
Hsp92II CATG 6 cut(s) 13, 287, 486, 503, 547, 1107
HspAI GCGC 1 cut(s) 873
Ksp22I TGATCA 1 cut(s) 622
Kzo9I GATC 6 cut(s) 404, 540, 622, 1100, 1130, 1140
LguI GCTCTTC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 561
LweI GCATC 1 cut(s) 736
MaeI CTAG 1 cut(s) 803
MaeII ACGT 2 cut(s) 120, 686
MaeIII GTNAC 5 cut(s) 165, 202, 511, 893, 904
MalI GATC 6 cut(s) 406, 542, 624, 1102, 1132, 1142
MboI GATC 6 cut(s) 404, 540, 622, 1100, 1130, 1140
MboII GAAGA 5 cut(s) 294, 410, 518, 629, 1058
MflI RGATCY 1 cut(s) 1140
MhlI GDGCHC 2 cut(s) 138, 653
MlsI TGGCCA 1 cut(s) 1053
MluCI AATT 6 cut(s) 129, 255, 436, 548, 760, 945
MluNI TGGCCA 1 cut(s) 1053
MlyI GAGTC 5 cut(s) 53, 323, 488, 641, 793
MmeI TCCRAC 3 cut(s) 330, 367, 776
MnlI CCTC 7 cut(s) 19, 45, 92, 100, 370, 456, 484
Mox20I TGGCCA 1 cut(s) 1053
Mph1103I ATGCAT 1 cut(s) 535
MscI TGGCCA 1 cut(s) 1053
MslI CAYNNNNRTG 1 cut(s) 966
Msp20I TGGCCA 1 cut(s) 1053
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 2 cut(s) 767, 1005
MvaI CCWGG 2 cut(s) 767, 1005
MvnI CGCG 1 cut(s) 922
NcoI CCATGG 1 cut(s) 9
NdeII GATC 6 cut(s) 404, 540, 622, 1100, 1130, 1140
NlaIII CATG 6 cut(s) 13, 287, 486, 503, 547, 1107
NlaIV GGNNCC 3 cut(s) 313, 934, 1009
NmuCI GTSAC 2 cut(s) 165, 202
NsiI ATGCAT 1 cut(s) 535
NspI RCATGY 2 cut(s) 287, 486
PaeI GCATGC 1 cut(s) 486
PciI ACATGT 1 cut(s) 283
PciSI GCTCTTC 1 cut(s) 307
PfoI TCCNGGA 1 cut(s) 765
PinAI ACCGGT 1 cut(s) 199
PkrI GCNGC 2 cut(s) 427, 959
PleI GAGTC 5 cut(s) 52, 323, 488, 640, 793
PpsI GAGTC 5 cut(s) 52, 323, 488, 640, 793
PpuMI RGGWCCY 1 cut(s) 1007
PscI ACATGT 1 cut(s) 283
Psp5II RGGWCCY 1 cut(s) 1007
Psp6I CCWGG 2 cut(s) 765, 1003
PspGI CCWGG 2 cut(s) 765, 1003
PspN4I GGNNCC 3 cut(s) 313, 934, 1009
PspPI GGNCC 1 cut(s) 1007
PspPPI RGGWCCY 1 cut(s) 1007
PsuI RGATCY 1 cut(s) 1140
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
RseI CAYNNNNRTG 1 cut(s) 966
SapI GCTCTTC 1 cut(s) 307
SatI GCNGC 2 cut(s) 426, 958
Sau3AI GATC 6 cut(s) 404, 540, 622, 1100, 1130, 1140
Sau96I GGNCC 1 cut(s) 1007
ScaI AGTACT 1 cut(s) 215
SchI GAGTC 5 cut(s) 53, 323, 488, 641, 793
ScrFI CCNGG 2 cut(s) 767, 1005
SduI GDGCHC 2 cut(s) 138, 653
SfaNI GCATC 1 cut(s) 736
SfcI CTRYAG 1 cut(s) 570
SgrAI CRCCGGYG 1 cut(s) 199
SinI GGWCC 1 cut(s) 1007
SmiMI CAYNNNNRTG 1 cut(s) 966
SmlI CTYRAG 2 cut(s) 629, 838
SmoI CTYRAG 2 cut(s) 629, 838
SphI GCATGC 1 cut(s) 486
Sse9I AATT 6 cut(s) 129, 255, 436, 548, 760, 945
SsiI CCGC 4 cut(s) 161, 334, 426, 957
SspMI CTAG 1 cut(s) 803
StyD4I CCNGG 2 cut(s) 765, 1003
StyI CCWWGG 3 cut(s) 9, 138, 1012
TaaI ACNGT 3 cut(s) 171, 794, 1079
TaiI ACGT 2 cut(s) 123, 689
TaqI TCGA 1 cut(s) 371
TasI AATT 6 cut(s) 129, 255, 436, 548, 760, 945
TatI WGTACW 1 cut(s) 213
TauI GCSGC 2 cut(s) 428, 960
TscAI CASTG 4 cut(s) 174, 189, 642, 886
TseFI GTSAC 2 cut(s) 165, 202
Tsp45I GTSAC 2 cut(s) 165, 202
TspDTI ATGAA 1 cut(s) 249
TspGWI ACGGA 1 cut(s) 324
TspRI CASTG 4 cut(s) 174, 189, 642, 886
VpaK11BI GGWCC 1 cut(s) 1007
XapI RAATTY 3 cut(s) 255, 548, 760
XceI RCATGY 2 cut(s) 287, 486
XcmI CCANNNNNNNNNTGG 2 cut(s) 664, 1025
XspI CTAG 1 cut(s) 803
ZrmI AGTACT 1 cut(s) 215
Zsp2I ATGCAT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.