Rw6G030530

WD repeat-containing protein 89 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009953
Physical Location & Seq
Reverse (-)
54641308 .. 54643659
2352 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G030530.1

Sequence Viewer

Length: 1152 bp
ATGGAAGCCATGGACTTGGATATGGAATCGAAACCTCAACCGCAGGACTCATCCAAGCGTTTCGGCCTCAAAACCTCTATCCCAACCAACTTTGGAGACGATTACGTCTTCCAAATTGTGCCCAAGGATGATTGGACAGCAATGGCCGTGTCACTGTCAACCAACGCAGTGAAGCTCTACTCACCGGTGACTGGTCAGTACTACGGAGAGTGCAAAGGCCACTCTGCAACTATCAATCAAATTTCATTTGCAGACCCATCTTCCCCACATGTTTTGCACTCCTGCTCTTCCGACGGAACCATTAGACTTTGGGACTCCCGCACGTTTCAACAGGTGTCATCTTTTCACTCTGGTTCGAGTGAGGAGATTTTCAGCTTTTCGTTTGGTGGATCATCTAATAATCTTCTGGCCGCAGGATGTAATTCTCAGATACTAATGTGGGATTGGAGGAACGACAAACAAGTAGCATGCTTAGAGGACTCTCATGTGGAAGATGTTACTCAGGTTCACTTCATTCCTGACCATCAAAGCAAGCTCCTTTCTGCTTCTGTAGATGGATTGATATGTATATTTGATACAGATGGAGATATCAACGAAGATGATCATCTTGAGTCAGTGCTAAATGTGGGCACTTCCATTGGGAAGGTGGGGTTTTTTGGTGAGACGTATCAAAAGCTTTGGTGTTTGACCCATATTGAAACCTTAAGTATCTGGGACTGGAAGGAAGCAACTGAAACAAATTTCCAGGACGCTCGTTCGTTGGCTTCTAACAGTTGGACTCTAGGTGAAGTTGATTATTTTGTTGATTGCCACTACTCAAGAGAAGCAGAACGATTATGGCTTATTGGTGGCGCCAACACTGGCACTTTAGGTTACTTCCCTGTAACTTACACAGGAACGCGAGCCATAGGTTCCCCTGAAGCAATTATGGAAGGTGGCCATACGGGTGTTGTCCGAAGTGTGTTGCCTATGTCAAACATGCCAGGTCCCCAAGGCAAAAGCATTTTTGGATGGACTGGCGGTGAAGATGGCCATTTATGTTGCTGGTTATCAGATGATTCTCTGGACACCATAAACCGATCATGGAGTTCAAGCGAACTGGTTTTGAGATCACCAAAGATCCGAAAGTCAAAAAGGCATAGTCCCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010029 GO:0040008 GO:0048580 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:1900140 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

383

Amino Acids

42.42

Weight (kDa)

4.89

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 33 - 242 2.9e-12 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 36 - 193 5.2e-10 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 46 - 194 2.5e-23 WDR5 beta-propeller domain
WDR55 PF24796 46 - 244 6e-10 WDR55
Beta-prop_WDR3_1st PF25173 50 - 194 1.2e-16 WDR3 first beta-propeller domain
Beta-prop_WDR75_1st PF23869 51 - 106 6.1e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_THOC3 PF25174 55 - 151 3.5e-11 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 55 - 149 7.4e-08 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 56 - 192 1.6e-12 Prp19 WD40 domain
WD40 PF00400 68 - 105 4e-06 WD domain, G-beta repeat
WD40_Gbeta PF25391 72 - 196 1.6e-07 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 86 - 201 6.6e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 92 - 194 3.6e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 97 - 189 1.4e-07 WDR36/Utp21 first beta-propeller
Beta-prop_EML PF23409 97 - 181 1e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EIPR1 PF23609 107 - 193 3e-06 EIPR1 beta-propeller
Beta-prop_THOC3 PF25174 123 - 194 8.2e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 104
AccB1I GGYRCC 1 cut(s) 851
AccII CGCG 1 cut(s) 901
AciI CCGC 4 cut(s) 41, 319, 411, 1020
AclWI GGATC 2 cut(s) 397, 1114
AcoI YGGCCR 4 cut(s) 144, 408, 937, 1030
AcsI RAATTY 2 cut(s) 240, 739
AcuI CTGAAG 1 cut(s) 939
AcyI GRCGYC 1 cut(s) 852
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 1 cut(s) 191
AflII CTTAAG 1 cut(s) 703
AflIII ACRYGT 1 cut(s) 268
AgeI ACCGGT 1 cut(s) 184
AgsI TTSAA 3 cut(s) 329, 698, 1092
AjnI CCWGG 2 cut(s) 744, 982
AluBI AGCT 4 cut(s) 175, 375, 535, 676
AluI AGCT 4 cut(s) 175, 375, 535, 676
Alw26I GTCTC 2 cut(s) 90, 656
AlwI GGATC 2 cut(s) 397, 1114
AoxI GGCC 6 cut(s) 64, 144, 217, 408, 937, 1030
ApoI RAATTY 2 cut(s) 240, 739
AsiGI ACCGGT 1 cut(s) 184
AspLEI GCGC 1 cut(s) 854
AspS9I GGNCC 1 cut(s) 986
AsuHPI GGTGA 6 cut(s) 174, 199, 671, 797, 1034, 1104
AvaII GGWCC 1 cut(s) 986
BaeGI GKGCMC 2 cut(s) 123, 632
BaeI ACNNNNGTAYC 2 cut(s) 422, 455
BalI TGGCCA 2 cut(s) 939, 1032
BanI GGYRCC 1 cut(s) 851
BbsI GAAGAC 1 cut(s) 100
BccI CCATC 6 cut(s) 265, 531, 548, 575, 1005, 1022
BceAI ACGGC 1 cut(s) 131
BciT130I CCWGG 2 cut(s) 746, 984
BclI TGATCA 1 cut(s) 601
BcoDI GTCTC 2 cut(s) 90, 656
BfaI CTAG 2 cut(s) 782, 1150
BfmI CTRYAG 1 cut(s) 549
BfoI RGCGCY 1 cut(s) 855
BfrI CTTAAG 1 cut(s) 703
BisI GCNGC 1 cut(s) 411
BlsI GCNGC 1 cut(s) 412
BmcAI AGTACT 1 cut(s) 200
Bme1390I CCNGG 2 cut(s) 746, 984
Bme18I GGWCC 1 cut(s) 986
BmgT120I GGNCC 1 cut(s) 986
BmiI GGNNCC 4 cut(s) 298, 853, 913, 988
BmrFI CCNGG 2 cut(s) 746, 984
BpiI GAAGAC 1 cut(s) 100
BpuEI CTTGAG 2 cut(s) 629, 802
BsaBI GATNNNNATC 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 852
BsaJI CCNNGG 3 cut(s) 9, 123, 991
BsaWI WCCGGW 1 cut(s) 184
Bsc4I CCNNNNNNNGG 1 cut(s) 191
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 5 cut(s) 196, 722, 865, 1021, 1104
Bse3DI GCAATG 1 cut(s) 147
Bse8I GATNNNNATC 1 cut(s) 603
BseBI CCWGG 2 cut(s) 746, 984
BseDI CCNNGG 3 cut(s) 9, 123, 991
BseGI GGATG 4 cut(s) 50, 133, 422, 1016
BseJI GATNNNNATC 1 cut(s) 603
BseLI CCNNNNNNNGG 1 cut(s) 191
BseMI GCAATG 1 cut(s) 147
BseMII CTCAG 2 cut(s) 440, 515
BseNI ACTGG 5 cut(s) 196, 722, 865, 1021, 1104
BseRI GAGGAG 1 cut(s) 377
BseSI GKGCMC 2 cut(s) 123, 632
Bsh1236I CGCG 1 cut(s) 901
BshFI GGCC 6 cut(s) 66, 146, 219, 410, 939, 1032
BshNI GGYRCC 1 cut(s) 851
BshTI ACCGGT 1 cut(s) 184
BsiSI CCGG 1 cut(s) 185
BslFI GGGAC 4 cut(s) 326, 728, 972, 1128
BslI CCNNNNNNNGG 1 cut(s) 191
BsmAI GTCTC 2 cut(s) 90, 656
BsmBI CGTCTC 2 cut(s) 90, 656
BsmFI GGGAC 4 cut(s) 326, 728, 972, 1128
BsnI GGCC 6 cut(s) 66, 146, 219, 410, 939, 1032
Bsp1286I GDGCHC 2 cut(s) 123, 632
Bsp143I GATC 5 cut(s) 389, 601, 1079, 1109, 1119
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 4 cut(s) 41, 319, 411, 1020
BspANI GGCC 6 cut(s) 66, 146, 219, 410, 939, 1032
BspCNI CTCAG 2 cut(s) 439, 514
BspFNI CGCG 1 cut(s) 901
BspLI GGNNCC 4 cut(s) 298, 853, 913, 988
BspPI GGATC 2 cut(s) 397, 1114
BspQI GCTCTTC 1 cut(s) 292
BspT107I GGYRCC 1 cut(s) 851
BspTI CTTAAG 1 cut(s) 703
BsrDI GCAATG 1 cut(s) 147
BsrFI RCCGGY 1 cut(s) 184
BsrI ACTGG 5 cut(s) 196, 722, 865, 1021, 1104
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 3 cut(s) 9, 123, 991
BssMI GATC 5 cut(s) 389, 601, 1079, 1109, 1119
BssNI GRCGYC 1 cut(s) 852
BssT1I CCWWGG 3 cut(s) 9, 123, 991
Bst2UI CCWGG 2 cut(s) 746, 984
Bst4CI ACNGT 2 cut(s) 156, 773
Bst6I CTCTTC 1 cut(s) 292
BstACI GRCGYC 1 cut(s) 852
BstAFI CTTAAG 1 cut(s) 703
BstC8I GCNNGC 3 cut(s) 469, 533, 903
BstDEI CTNAG 3 cut(s) 426, 472, 501
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 4 cut(s) 50, 133, 422, 1016
BstFNI CGCG 1 cut(s) 901
BstH2I RGCGCY 1 cut(s) 855
BstHHI GCGC 1 cut(s) 854
BstKTI GATC 5 cut(s) 392, 604, 1082, 1112, 1122
BstMAI GTCTC 2 cut(s) 90, 656
BstMBI GATC 5 cut(s) 389, 601, 1079, 1109, 1119
BstNI CCWGG 2 cut(s) 746, 984
BstNSI RCATGY 3 cut(s) 272, 471, 982
BstSCI CCNGG 2 cut(s) 744, 982
BstSFI CTRYAG 1 cut(s) 549
BstSLI GKGCMC 2 cut(s) 123, 632
BstUI CGCG 1 cut(s) 901
BstV2I GAAGAC 1 cut(s) 100
BstX2I RGATCY 1 cut(s) 1119
BstXI CCANNNNNNTGG 1 cut(s) 16
BstYI RGATCY 1 cut(s) 1119
BsuRI GGCC 6 cut(s) 66, 146, 219, 410, 939, 1032
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 4 cut(s) 50, 133, 422, 1016
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 4 cut(s) 152, 174, 621, 858
Cac8I GCNNGC 3 cut(s) 469, 533, 903
CfoI GCGC 1 cut(s) 854
Cfr10I RCCGGY 1 cut(s) 184
Cfr13I GGNCC 1 cut(s) 986
CseI GACGC 1 cut(s) 758
Csp6I GTAC 1 cut(s) 199
CspAI ACCGGT 1 cut(s) 184
CspCI CAANNNNNGTGG 2 cut(s) 255, 290
CviAII CATG 6 cut(s) 10, 269, 468, 485, 979, 1083
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 3 cut(s) 426, 472, 501
DinI GGCGCC 1 cut(s) 853
DpnI GATC 5 cut(s) 391, 603, 1081, 1111, 1121
DpnII GATC 5 cut(s) 389, 601, 1079, 1109, 1119
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 4 cut(s) 144, 408, 937, 1030
Eam1104I CTCTTC 1 cut(s) 292
EarI CTCTTC 1 cut(s) 292
Eco130I CCWWGG 3 cut(s) 9, 123, 991
Eco32I GATATC 1 cut(s) 589
Eco47I GGWCC 1 cut(s) 986
Eco57I CTGAAG 1 cut(s) 939
EcoO109I RGGNCCY 1 cut(s) 986
EcoRII CCWGG 2 cut(s) 744, 982
EcoRV GATATC 1 cut(s) 589
EcoT14I CCWWGG 3 cut(s) 9, 123, 991
EgeI GGCGCC 1 cut(s) 853
EheI GGCGCC 1 cut(s) 853
ErhI CCWWGG 3 cut(s) 9, 123, 991
Esp3I CGTCTC 2 cut(s) 90, 656
FaeI CATG 6 cut(s) 13, 272, 471, 488, 982, 1086
FaqI GGGAC 4 cut(s) 326, 728, 972, 1128
FatI CATG 6 cut(s) 9, 268, 467, 484, 978, 1082
FauI CCCGC 1 cut(s) 326
FbaI TGATCA 1 cut(s) 601
Fnu4HI GCNGC 1 cut(s) 411
FokI GGATG 4 cut(s) 37, 140, 429, 1023
Fsp4HI GCNGC 1 cut(s) 411
FspBI CTAG 2 cut(s) 782, 1150
GlaI GCGC 1 cut(s) 853
GluI GCNGC 1 cut(s) 411
HaeII RGCGCY 1 cut(s) 855
HaeIII GGCC 6 cut(s) 66, 146, 219, 410, 939, 1032
HapII CCGG 1 cut(s) 185
HgaI GACGC 1 cut(s) 758
HhaI GCGC 1 cut(s) 854
Hin1I GRCGYC 1 cut(s) 852
Hin1II CATG 6 cut(s) 13, 272, 471, 488, 982, 1086
Hin6I GCGC 1 cut(s) 852
HinP1I GCGC 1 cut(s) 852
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HindIII AAGCTT 1 cut(s) 674
HinfI GANTC 7 cut(s) 26, 47, 314, 479, 611, 778, 1058
HpaII CCGG 1 cut(s) 185
HphI GGTGA 6 cut(s) 174, 199, 671, 797, 1034, 1104
Hpy166II GTNNAC 2 cut(s) 159, 508
Hpy188I TCNGA 5 cut(s) 292, 429, 956, 1054, 1124
Hpy188III TCNNGA 4 cut(s) 518, 608, 819, 1064
Hpy8I GTNNAC 2 cut(s) 159, 508
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 3 cut(s) 637, 715, 926
HpyCH4III ACNGT 2 cut(s) 156, 773
HpyCH4IV ACGT 3 cut(s) 105, 323, 665
HpyCH4V TGCA 4 cut(s) 213, 227, 251, 277
HpyF3I CTNAG 3 cut(s) 426, 472, 501
HpySE526I ACGT 3 cut(s) 105, 323, 665
Hsp92I GRCGYC 1 cut(s) 852
Hsp92II CATG 6 cut(s) 13, 272, 471, 488, 982, 1086
HspAI GCGC 1 cut(s) 852
KasI GGCGCC 1 cut(s) 851
Ksp22I TGATCA 1 cut(s) 601
Kzo9I GATC 5 cut(s) 389, 601, 1079, 1109, 1119
LguI GCTCTTC 1 cut(s) 292
LmnI GCTCC 1 cut(s) 540
MaeI CTAG 2 cut(s) 782, 1150
MaeII ACGT 3 cut(s) 105, 323, 665
MaeIII GTNAC 5 cut(s) 150, 187, 496, 872, 883
MalI GATC 5 cut(s) 391, 603, 1081, 1111, 1121
MboI GATC 5 cut(s) 389, 601, 1079, 1109, 1119
MboII GAAGA 7 cut(s) 100, 252, 279, 395, 503, 608, 1037
MflI RGATCY 1 cut(s) 1119
MhlI GDGCHC 2 cut(s) 123, 632
MlsI TGGCCA 2 cut(s) 939, 1032
MluCI AATT 5 cut(s) 114, 240, 421, 739, 924
MluNI TGGCCA 2 cut(s) 939, 1032
Mly113I GGCGCC 1 cut(s) 852
MlyI GAGTC 5 cut(s) 41, 308, 473, 620, 772
MmeI TCCRAC 2 cut(s) 315, 755
MnlI CCTC 6 cut(s) 45, 77, 85, 355, 441, 469
Mox20I TGGCCA 2 cut(s) 939, 1032
MscI TGGCCA 2 cut(s) 939, 1032
MseI TTAA 1 cut(s) 704
MslI CAYNNNNRTG 1 cut(s) 945
Msp20I TGGCCA 2 cut(s) 939, 1032
MspCI CTTAAG 1 cut(s) 703
MspI CCGG 1 cut(s) 185
MspR9I CCNGG 2 cut(s) 746, 984
MvaI CCWGG 2 cut(s) 746, 984
MvnI CGCG 1 cut(s) 901
NarI GGCGCC 1 cut(s) 852
NcoI CCATGG 1 cut(s) 9
NdeII GATC 5 cut(s) 389, 601, 1079, 1109, 1119
NlaIII CATG 6 cut(s) 13, 272, 471, 488, 982, 1086
NlaIV GGNNCC 4 cut(s) 298, 853, 913, 988
NmuCI GTSAC 2 cut(s) 150, 187
NspI RCATGY 3 cut(s) 272, 471, 982
PaeI GCATGC 1 cut(s) 471
PciI ACATGT 1 cut(s) 268
PciSI GCTCTTC 1 cut(s) 292
PfeI GAWTC 2 cut(s) 26, 1058
PfoI TCCNGGA 1 cut(s) 744
PinAI ACCGGT 1 cut(s) 184
PkrI GCNGC 1 cut(s) 412
PleI GAGTC 5 cut(s) 41, 308, 473, 619, 772
PluTI GGCGCC 1 cut(s) 855
PpsI GAGTC 5 cut(s) 41, 308, 473, 619, 772
PpuMI RGGWCCY 1 cut(s) 986
PscI ACATGT 1 cut(s) 268
Psp5II RGGWCCY 1 cut(s) 986
Psp6I CCWGG 2 cut(s) 744, 982
PspGI CCWGG 2 cut(s) 744, 982
PspN4I GGNNCC 4 cut(s) 298, 853, 913, 988
PspPI GGNCC 1 cut(s) 986
PspPPI RGGWCCY 1 cut(s) 986
PsuI RGATCY 1 cut(s) 1119
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 945
SapI GCTCTTC 1 cut(s) 292
SaqAI TTAA 1 cut(s) 704
SatI GCNGC 1 cut(s) 411
Sau3AI GATC 5 cut(s) 389, 601, 1079, 1109, 1119
Sau96I GGNCC 1 cut(s) 986
ScaI AGTACT 1 cut(s) 200
SchI GAGTC 5 cut(s) 41, 308, 473, 620, 772
ScrFI CCNGG 2 cut(s) 746, 984
SduI GDGCHC 2 cut(s) 123, 632
SfcI CTRYAG 1 cut(s) 549
SfoI GGCGCC 1 cut(s) 853
SgrAI CRCCGGYG 1 cut(s) 184
SinI GGWCC 1 cut(s) 986
SmiMI CAYNNNNRTG 1 cut(s) 945
SmlI CTYRAG 3 cut(s) 608, 703, 817
SmoI CTYRAG 3 cut(s) 608, 703, 817
SphI GCATGC 1 cut(s) 471
Sse9I AATT 5 cut(s) 114, 240, 421, 739, 924
SsiI CCGC 4 cut(s) 41, 319, 411, 1020
SspDI GGCGCC 1 cut(s) 851
SspMI CTAG 2 cut(s) 782, 1150
StyD4I CCNGG 2 cut(s) 744, 982
StyI CCWWGG 3 cut(s) 9, 123, 991
TaaI ACNGT 2 cut(s) 156, 773
TaiI ACGT 3 cut(s) 108, 326, 668
TaqI TCGA 2 cut(s) 29, 356
TasI AATT 5 cut(s) 114, 240, 421, 739, 924
TatI WGTACW 1 cut(s) 198
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 2 cut(s) 26, 1058
Tru1I TTAA 1 cut(s) 704
Tru9I TTAA 1 cut(s) 704
TscAI CASTG 4 cut(s) 159, 174, 621, 865
TseFI GTSAC 2 cut(s) 150, 187
Tsp45I GTSAC 2 cut(s) 150, 187
TspDTI ATGAA 2 cut(s) 234, 502
TspGWI ACGGA 2 cut(s) 219, 309
TspRI CASTG 4 cut(s) 159, 174, 621, 865
Vha464I CTTAAG 1 cut(s) 703
VpaK11BI GGWCC 1 cut(s) 986
XapI RAATTY 2 cut(s) 240, 739
XceI RCATGY 3 cut(s) 272, 471, 982
XcmI CCANNNNNNNNNTGG 1 cut(s) 643
XspI CTAG 2 cut(s) 782, 1150
ZrmI AGTACT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.