FvH4_2g25580

Ribosome biogenesis protein Nop16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009327
Physical Location & Seq
Forward (+)
20701788 .. 20703713
1926 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25580.t1

Sequence Viewer

Length: 537 bp
ATGGGAGGTTCAAGAAGAAAGTACAAGCGATCTCGGACCAAGGTGCGGGTGGCGCTGCCGAAGCGTAAGCCCAACGTCTTCAAGCCGGCCTTCTGTCTCCCTCCGAAGCTCCGGGCCTTGATCGACCAAGCAGACCCTGCCTCTCGCCCTAAGTGGGACCCCGAAGGCACCGTCATCCAAAACTACAAGTCGTTCGGCGTCCTCTCCAACCCTAACTCTCTCCAGGTCACTCCTGATCAGCCTGACCCCGCCGAGCTCGTAGACCCCAACGACGACGGTAGTGATCTCGAAGAGGACGATTTAAAAACTACTCTGGGAAAGAAACGGAGAGATGGGAGACCTGCTTCGTTACTACCGTTAACCACCATCCAACGTGTTCATGTCAGTCGGCTTATTGAGAAATATGGAGATGACTATCAGAGAATGTTCATGGATACAAAACTAAATGCAATGCAGCATTCTGTTGCAACTTTGGAGAAGTTATGCAGAAGGTATCATATGTATAAAGATCGAAATCCCTTGATATTGACCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nop16

Protein Analysis

179

Amino Acids

20.46

Weight (kDa)

9.85

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop16 PF09420 100 - 166 4.5e-15 Ribosome biogenesis protein Nop16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 349
AccB1I GGYRCC 1 cut(s) 167
AccI GTMKAC 1 cut(s) 261
AciI CCGC 2 cut(s) 46, 249
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 1 cut(s) 23
AfiI CCNNNNNNNGG 2 cut(s) 45, 154
AflIII ACRYGT 1 cut(s) 373
AgsI TTSAA 2 cut(s) 12, 82
AjnI CCWGG 1 cut(s) 222
AluBI AGCT 2 cut(s) 109, 256
AluI AGCT 2 cut(s) 109, 256
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 2 cut(s) 101, 331
AlwNI CAGNNNCTG 1 cut(s) 137
AoxI GGCC 2 cut(s) 87, 114
ApeKI GCWGC 2 cut(s) 55, 454
ArsI GACNNNNNNTTYG 2 cut(s) 156, 188
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 3 cut(s) 36, 114, 157
AsuC2I CCSGG 1 cut(s) 113
AvaII GGWCC 2 cut(s) 36, 157
BanI GGYRCC 1 cut(s) 167
BanII GRGCYC 1 cut(s) 258
BbsI GAAGAC 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 2 cut(s) 42, 466
BccI CCATC 2 cut(s) 326, 374
BciT130I CCWGG 1 cut(s) 224
BciVI GTATCC 1 cut(s) 427
BclI TGATCA 1 cut(s) 235
BcnI CCSGG 1 cut(s) 113
BcoDI GTCTC 2 cut(s) 101, 331
BfoI RGCGCY 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 349
BfuI GTATCC 1 cut(s) 427
BisI GCNGC 2 cut(s) 56, 455
BlsI GCNGC 2 cut(s) 57, 456
Bme1390I CCNGG 2 cut(s) 113, 224
Bme18I GGWCC 2 cut(s) 36, 157
BmgT120I GGNCC 3 cut(s) 36, 114, 157
BmiI GGNNCC 3 cut(s) 158, 159, 169
BmrFI CCNGG 2 cut(s) 113, 224
BpiI GAAGAC 1 cut(s) 70
BpmI CTGGAG 1 cut(s) 206
BpuMI CCSGG 1 cut(s) 113
BsaBI GATNNNNATC 2 cut(s) 414, 513
BsaHI GRCGYC 1 cut(s) 198
BsaI GGTCTC 1 cut(s) 331
BsaJI CCNNGG 1 cut(s) 39
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 154
Bse118I RCCGGY 1 cut(s) 85
Bse3DI GCAATG 1 cut(s) 456
Bse8I GATNNNNATC 2 cut(s) 414, 513
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 2 cut(s) 174, 366
BseJI GATNNNNATC 2 cut(s) 414, 513
BseLI CCNNNNNNNGG 2 cut(s) 45, 154
BseMI GCAATG 1 cut(s) 456
BseXI GCAGC 2 cut(s) 42, 466
BshFI GGCC 2 cut(s) 89, 116
BshNI GGYRCC 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 2 cut(s) 86, 112
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 2 cut(s) 45, 154
BsmAI GTCTC 2 cut(s) 101, 331
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 1 cut(s) 457
BsnI GGCC 2 cut(s) 89, 116
Bso31I GGTCTC 1 cut(s) 331
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 5 cut(s) 29, 120, 235, 283, 508
BspACI CCGC 2 cut(s) 46, 249
BspANI GGCC 2 cut(s) 89, 116
BspLI GGNNCC 3 cut(s) 158, 159, 169
BspMI ACCTGC 1 cut(s) 349
BspT107I GGYRCC 1 cut(s) 167
BspTNI GGTCTC 1 cut(s) 331
BsrDI GCAATG 1 cut(s) 456
BsrFI RCCGGY 1 cut(s) 85
BssAI RCCGGY 1 cut(s) 85
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 5 cut(s) 29, 120, 235, 283, 508
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 224
Bst4CI ACNGT 3 cut(s) 172, 278, 357
Bst6I CTCTTC 1 cut(s) 285
BstACI GRCGYC 1 cut(s) 198
BstAPI GCANNNNNTGC 1 cut(s) 137
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 1 cut(s) 150
BstF5I GGATG 2 cut(s) 174, 366
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 5 cut(s) 32, 123, 238, 286, 511
BstMAI GTCTC 2 cut(s) 101, 331
BstMBI GATC 5 cut(s) 29, 120, 235, 283, 508
BstMWI GCNNNNNNNGC 3 cut(s) 52, 61, 137
BstNI CCWGG 1 cut(s) 224
BstSCI CCNGG 2 cut(s) 111, 222
BstV1I GCAGC 2 cut(s) 42, 466
BstV2I GAAGAC 1 cut(s) 70
BsuI GTATCC 1 cut(s) 427
BsuRI GGCC 2 cut(s) 89, 116
BtsCI GGATG 2 cut(s) 174, 366
BveI ACCTGC 1 cut(s) 349
Cac8I GCNNGC 1 cut(s) 87
CaiI CAGNNNCTG 1 cut(s) 137
CfoI GCGC 1 cut(s) 55
Cfr10I RCCGGY 1 cut(s) 85
Cfr13I GGNCC 3 cut(s) 36, 114, 157
CseI GACGC 1 cut(s) 187
Csp6I GTAC 1 cut(s) 22
CviAII CATG 2 cut(s) 380, 430
CviJI RGCY 8 cut(s) 70, 85, 89, 109, 116, 241, 256, 391
CviKI_1 RGCY 8 cut(s) 70, 85, 89, 109, 116, 241, 256, 391
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 1 cut(s) 150
DpnI GATC 5 cut(s) 31, 122, 237, 285, 510
DpnII GATC 5 cut(s) 29, 120, 235, 283, 508
DraI TTTAAA 1 cut(s) 303
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
Ecl136II GAGCTC 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 258
Eco31I GGTCTC 1 cut(s) 331
Eco47I GGWCC 2 cut(s) 36, 157
Eco53kI GAGCTC 1 cut(s) 256
EcoICRI GAGCTC 1 cut(s) 256
EcoO109I RGGNCCY 1 cut(s) 157
EcoRII CCWGG 1 cut(s) 222
EcoT14I CCWWGG 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 2 cut(s) 383, 433
FaiI YATR 7 cut(s) 381, 405, 431, 484, 498, 500, 504
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 1 cut(s) 170
FatI CATG 2 cut(s) 379, 429
FauI CCCGC 2 cut(s) 39, 256
FauNDI CATATG 1 cut(s) 498
FbaI TGATCA 1 cut(s) 235
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 2 cut(s) 56, 455
FokI GGATG 2 cut(s) 161, 353
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 2 cut(s) 56, 455
GlaI GCGC 1 cut(s) 54
GluI GCNGC 2 cut(s) 56, 455
GsuI CTGGAG 1 cut(s) 206
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 2 cut(s) 89, 116
HapII CCGG 2 cut(s) 86, 112
HgaI GACGC 1 cut(s) 187
HhaI GCGC 1 cut(s) 55
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 2 cut(s) 383, 433
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HincII GTYRAC 1 cut(s) 360
HindII GTYRAC 1 cut(s) 360
HpaI GTTAAC 1 cut(s) 360
HpaII CCGG 2 cut(s) 86, 112
Hpy166II GTNNAC 2 cut(s) 262, 360
Hpy188I TCNGA 3 cut(s) 36, 105, 420
Hpy188III TCNNGA 3 cut(s) 12, 233, 287
Hpy8I GTNNAC 2 cut(s) 262, 360
Hpy99I CGWCG 2 cut(s) 275, 278
HpyAV CCTTC 3 cut(s) 100, 158, 483
HpyCH4III ACNGT 3 cut(s) 172, 278, 357
HpyCH4IV ACGT 2 cut(s) 75, 373
HpyCH4V TGCA 4 cut(s) 449, 454, 467, 486
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 61, 137
HpyF3I CTNAG 1 cut(s) 150
HpySE526I ACGT 2 cut(s) 75, 373
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 2 cut(s) 383, 433
HspAI GCGC 1 cut(s) 53
KflI GGGWCCC 1 cut(s) 157
KroI GCCGGC 1 cut(s) 85
KroNI GCCGGC 1 cut(s) 87
Ksp22I TGATCA 1 cut(s) 235
KspAI GTTAAC 1 cut(s) 360
Kzo9I GATC 5 cut(s) 29, 120, 235, 283, 508
LmnI GCTCC 1 cut(s) 114
LpnPI CCDG 9 cut(s) 99, 125, 150, 209, 236, 246, 255, 299, 354
Lsp1109I GCAGC 2 cut(s) 42, 466
MaeII ACGT 2 cut(s) 75, 373
MaeIII GTNAC 2 cut(s) 226, 348
MalI GATC 5 cut(s) 31, 122, 237, 285, 510
MboI GATC 5 cut(s) 29, 120, 235, 283, 508
MboII GAAGA 3 cut(s) 27, 70, 302
MhlI GDGCHC 1 cut(s) 258
MmeI TCCRAC 2 cut(s) 231, 394
MnlI CCTC 4 cut(s) 111, 151, 212, 286
MroNI GCCGGC 1 cut(s) 85
MseI TTAA 2 cut(s) 302, 359
MspI CCGG 2 cut(s) 86, 112
MspR9I CCNGG 2 cut(s) 113, 224
Mva1269I GAATGC 1 cut(s) 457
MvaI CCWGG 1 cut(s) 224
MwoI GCNNNNNNNGC 3 cut(s) 52, 61, 137
NaeI GCCGGC 1 cut(s) 87
NciI CCSGG 1 cut(s) 113
NdeI CATATG 1 cut(s) 498
NdeII GATC 5 cut(s) 29, 120, 235, 283, 508
NgoMIV GCCGGC 1 cut(s) 85
NlaIII CATG 2 cut(s) 383, 433
NlaIV GGNNCC 3 cut(s) 158, 159, 169
NmeAIII GCCGAG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 226
PcsI WCGNNNNNNNCGW 2 cut(s) 294, 353
PctI GAATGC 1 cut(s) 457
PdiI GCCGGC 1 cut(s) 87
PkrI GCNGC 2 cut(s) 57, 456
PpuMI RGGWCCY 1 cut(s) 157
Psp124BI GAGCTC 1 cut(s) 258
Psp5II RGGWCCY 1 cut(s) 157
Psp6I CCWGG 1 cut(s) 222
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 3 cut(s) 158, 159, 169
PspPI GGNCC 3 cut(s) 36, 114, 157
PspPPI RGGWCCY 1 cut(s) 157
PstNI CAGNNNCTG 1 cut(s) 137
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
SacI GAGCTC 1 cut(s) 258
SaqAI TTAA 2 cut(s) 302, 359
SatI GCNGC 2 cut(s) 56, 455
Sau3AI GATC 5 cut(s) 29, 120, 235, 283, 508
Sau96I GGNCC 3 cut(s) 36, 114, 157
ScrFI CCNGG 2 cut(s) 113, 224
SduI GDGCHC 1 cut(s) 258
SetI ASST 9 cut(s) 10, 45, 78, 111, 228, 258, 343, 376, 494
SinI GGWCC 2 cut(s) 36, 157
SsiI CCGC 2 cut(s) 46, 249
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 2 cut(s) 111, 222
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 3 cut(s) 172, 278, 357
TaiI ACGT 2 cut(s) 78, 376
TaqI TCGA 3 cut(s) 123, 288, 511
TatI WGTACW 1 cut(s) 21
Tru1I TTAA 2 cut(s) 302, 359
Tru9I TTAA 2 cut(s) 302, 359
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 2 cut(s) 55, 454
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 2 cut(s) 368, 418
TspGWI ACGGA 1 cut(s) 340
VpaK11BI GGWCC 2 cut(s) 36, 157
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.