MD11G1313700.v1.1

Ribosome biogenesis protein Nop16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009327
Physical Location & Seq
Reverse (-)
42601282 .. 42603552
2271 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1313700.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGGAAGGTCTAGAAGAAAGTACAAGCAGTCGCGGACGAAGGTGCGCGTGGGGCTGCCGAAGAGGAAGCCCAACGTATTCAAGCCCGCCTTCTGCCTCCCTCCGAAGCTCCGCGCCTTGCTGGCGCAGCAAGGGCCGGCCTCAGCCCCCCAATGGGACGCCGAGGGCTCCGTCATCCAAAACTACAAGTCCTTCGGCTTCGTCTCCAACCCTAACTTGCTCAGCCTCCGGTCTCGCAATTCTCACATGGTCGAGAGCGATTCCCTCCAGGTCCCCCCTCCGGCCTCCGATGACCCTGACGCCTCCGCAGAGCTCGATTTCGCCGACGGCAGCGATCTCGAAGAGGACGATTTGAAAACTAGTCTAGGGAAGAAACGTAGAGATGGAAAATCTGCACCACTACAACCATTAACGGCCATGCAGCGTCTTCATCTTGGTCGGCTCATTGAGGAGTATGGCGATGGCTATCAGAGAATGTTCATGGATACGAAACTAAATAAAATGCAGCATTCTGTCGCAACTTTGGAGAAATTATGCAGACGGTATCATGTGCATAAAGATAGAAATCCCTTGATATTGACCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nop16

Protein Analysis

196

Amino Acids

22.1

Weight (kDa)

9.88

Isoelectric Point (pI)

58.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop16 PF09420 114 - 183 3.2e-13 Ribosome biogenesis protein Nop16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 34, 48, 114
AciI CCGC 4 cut(s) 34, 87, 112, 306
AcoI YGGCCR 1 cut(s) 414
AcyI GRCGYC 2 cut(s) 159, 300
AfaI GTAC 1 cut(s) 23
AfiI CCNNNNNNNGG 3 cut(s) 153, 154, 280
AgsI TTSAA 2 cut(s) 82, 355
AhlI ACTAGT 1 cut(s) 359
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 2 cut(s) 109, 313
AluI AGCT 2 cut(s) 109, 313
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 208, 237
AoxI GGCC 4 cut(s) 134, 138, 282, 414
ApeKI GCWGC 5 cut(s) 55, 127, 330, 421, 505
AspLEI GCGC 3 cut(s) 48, 116, 127
AspS9I GGNCC 2 cut(s) 134, 271
AvaII GGWCC 1 cut(s) 271
BanII GRGCYC 2 cut(s) 170, 315
BbsI GAAGAC 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 315
BbvCI CCTCAGC 1 cut(s) 142
BbvI GCAGC 5 cut(s) 42, 139, 342, 433, 517
BccI CCATC 2 cut(s) 377, 455
BceAI ACGGC 2 cut(s) 343, 429
BcgI CGANNNNNNTGC 2 cut(s) 319, 353
BciT130I CCWGG 1 cut(s) 269
BciVI GTATCC 1 cut(s) 478
BcoDI GTCTC 2 cut(s) 208, 237
BcuI ACTAGT 1 cut(s) 359
BfaI CTAG 3 cut(s) 12, 360, 365
BfuI GTATCC 1 cut(s) 478
BglI GCCNNNNNGGC 1 cut(s) 122
BisI GCNGC 5 cut(s) 56, 128, 331, 422, 506
BlpI GCTNAGC 1 cut(s) 221
BlsI GCNGC 5 cut(s) 57, 129, 332, 423, 507
Bme1390I CCNGG 1 cut(s) 269
Bme18I GGWCC 1 cut(s) 271
BmgT120I GGNCC 2 cut(s) 134, 271
BmiI GGNNCC 2 cut(s) 169, 273
BmrFI CCNGG 1 cut(s) 269
BpiI GAAGAC 1 cut(s) 419
BpmI CTGGAG 1 cut(s) 251
Bpu10I CCTNAGC 1 cut(s) 142
Bpu1102I GCTNAGC 1 cut(s) 221
BsaBI GATNNNNATC 2 cut(s) 465, 564
BsaHI GRCGYC 2 cut(s) 159, 300
BsaI GGTCTC 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 162
BsaWI WCCGGW 1 cut(s) 228
Bsc4I CCNNNNNNNGG 3 cut(s) 153, 154, 280
Bse118I RCCGGY 1 cut(s) 136
Bse8I GATNNNNATC 2 cut(s) 465, 564
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 1 cut(s) 174
BseJI GATNNNNATC 2 cut(s) 465, 564
BseLI CCNNNNNNNGG 3 cut(s) 153, 154, 280
BseMII CTCAG 2 cut(s) 156, 235
BseRI GAGGAG 1 cut(s) 464
BseXI GCAGC 5 cut(s) 42, 139, 342, 433, 517
BsgI GTGCAG 1 cut(s) 378
Bsh1236I CGCG 3 cut(s) 34, 48, 114
BshFI GGCC 4 cut(s) 136, 140, 284, 416
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 3 cut(s) 137, 229, 281
BslFI GGGAC 2 cut(s) 170, 257
BslI CCNNNNNNNGG 3 cut(s) 153, 154, 280
BsmAI GTCTC 2 cut(s) 208, 237
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 2 cut(s) 170, 257
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 4 cut(s) 136, 140, 284, 416
Bso31I GGTCTC 1 cut(s) 237
Bsp1286I GDGCHC 2 cut(s) 170, 315
Bsp143I GATC 1 cut(s) 334
Bsp1720I GCTNAGC 1 cut(s) 221
BspACI CCGC 4 cut(s) 34, 87, 112, 306
BspANI GGCC 4 cut(s) 136, 140, 284, 416
BspCNI CTCAG 2 cut(s) 155, 234
BspFNI CGCG 3 cut(s) 34, 48, 114
BspLI GGNNCC 2 cut(s) 169, 273
BspTNI GGTCTC 1 cut(s) 237
BsrFI RCCGGY 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 1 cut(s) 334
BssNI GRCGYC 2 cut(s) 159, 300
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 1 cut(s) 543
Bst6I CTCTTC 2 cut(s) 56, 336
BstACI GRCGYC 2 cut(s) 159, 300
BstC8I GCNNGC 3 cut(s) 87, 123, 138
BstDEI CTNAG 2 cut(s) 142, 221
BstF5I GGATG 1 cut(s) 174
BstFNI CGCG 3 cut(s) 34, 48, 114
BstHHI GCGC 3 cut(s) 48, 116, 127
BstKTI GATC 1 cut(s) 337
BstMAI GTCTC 2 cut(s) 208, 237
BstMBI GATC 1 cut(s) 334
BstMWI GCNNNNNNNGC 4 cut(s) 52, 122, 127, 133
BstNI CCWGG 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 267
BstUI CGCG 3 cut(s) 34, 48, 114
BstV1I GCAGC 5 cut(s) 42, 139, 342, 433, 517
BstV2I GAAGAC 1 cut(s) 419
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 4 cut(s) 136, 140, 284, 416
BtgZI GCGATG 1 cut(s) 474
BtsCI GGATG 1 cut(s) 174
Cac8I GCNNGC 3 cut(s) 87, 123, 138
CfoI GCGC 3 cut(s) 48, 116, 127
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 2 cut(s) 134, 271
CseI GACGC 3 cut(s) 167, 308, 413
Csp6I GTAC 1 cut(s) 22
CviAII CATG 4 cut(s) 247, 418, 481, 548
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 2 cut(s) 142, 221
DpnI GATC 1 cut(s) 336
DpnII GATC 1 cut(s) 334
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 2 cut(s) 56, 336
EarI CTCTTC 2 cut(s) 56, 336
Ecl136II GAGCTC 1 cut(s) 313
Eco24I GRGCYC 2 cut(s) 170, 315
Eco31I GGTCTC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 271
Eco53kI GAGCTC 1 cut(s) 313
EcoICRI GAGCTC 1 cut(s) 313
EcoO109I RGGNCCY 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 267
EcoT38I GRGCYC 2 cut(s) 170, 315
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 4 cut(s) 250, 421, 484, 551
FaiI YATR 7 cut(s) 248, 419, 456, 482, 535, 549, 555
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 2 cut(s) 170, 257
FatI CATG 4 cut(s) 246, 417, 480, 547
FauI CCCGC 1 cut(s) 94
Fnu4HI GCNGC 5 cut(s) 56, 128, 331, 422, 506
FokI GGATG 1 cut(s) 161
FriOI GRGCYC 2 cut(s) 170, 315
FseI GGCCGGCC 1 cut(s) 140
Fsp4HI GCNGC 5 cut(s) 56, 128, 331, 422, 506
FspBI CTAG 3 cut(s) 12, 360, 365
GlaI GCGC 3 cut(s) 47, 115, 126
GluI GCNGC 5 cut(s) 56, 128, 331, 422, 506
GsuI CTGGAG 1 cut(s) 251
HaeIII GGCC 4 cut(s) 136, 140, 284, 416
HapII CCGG 3 cut(s) 137, 229, 281
HgaI GACGC 3 cut(s) 167, 308, 413
HhaI GCGC 3 cut(s) 48, 116, 127
Hin1I GRCGYC 2 cut(s) 159, 300
Hin1II CATG 4 cut(s) 250, 421, 484, 551
Hin6I GCGC 3 cut(s) 46, 114, 125
HinP1I GCGC 3 cut(s) 46, 114, 125
HinfI GANTC 1 cut(s) 260
HpaII CCGG 3 cut(s) 137, 229, 281
Hpy188I TCNGA 3 cut(s) 105, 289, 471
Hpy188III TCNNGA 3 cut(s) 12, 253, 338
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 3 cut(s) 34, 100, 202
HpyCH4III ACNGT 1 cut(s) 543
HpyCH4IV ACGT 2 cut(s) 75, 376
HpyCH4V TGCA 5 cut(s) 395, 421, 505, 537, 553
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 122, 127, 133
HpyF3I CTNAG 2 cut(s) 142, 221
HpySE526I ACGT 2 cut(s) 75, 376
Hsp92I GRCGYC 2 cut(s) 159, 300
Hsp92II CATG 4 cut(s) 250, 421, 484, 551
HspAI GCGC 3 cut(s) 46, 114, 125
KroI GCCGGC 1 cut(s) 136
KroNI GCCGGC 1 cut(s) 138
Kzo9I GATC 1 cut(s) 334
LmnI GCTCC 2 cut(s) 114, 173
LpnPI CCDG 7 cut(s) 107, 150, 242, 254, 281, 294, 309
Lsp1109I GCAGC 5 cut(s) 42, 139, 342, 433, 517
MaeI CTAG 3 cut(s) 12, 360, 365
MaeII ACGT 2 cut(s) 75, 376
MalI GATC 1 cut(s) 336
MboI GATC 1 cut(s) 334
MboII GAAGA 5 cut(s) 27, 73, 353, 382, 419
MhlI GDGCHC 2 cut(s) 170, 315
MluCI AATT 2 cut(s) 238, 530
MmeI TCCRAC 1 cut(s) 231
MroNI GCCGGC 1 cut(s) 136
MseI TTAA 1 cut(s) 410
MspI CCGG 3 cut(s) 137, 229, 281
MspR9I CCNGG 1 cut(s) 269
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 3 cut(s) 34, 48, 114
MwoI GCNNNNNNNGC 4 cut(s) 52, 122, 127, 133
NaeI GCCGGC 1 cut(s) 138
NdeII GATC 1 cut(s) 334
NgoMIV GCCGGC 1 cut(s) 136
NlaIII CATG 4 cut(s) 250, 421, 484, 551
NlaIV GGNNCC 2 cut(s) 169, 273
NmeAIII GCCGAG 1 cut(s) 187
PcsI WCGNNNNNNNCGW 2 cut(s) 321, 345
PctI GAATGC 1 cut(s) 508
PdiI GCCGGC 1 cut(s) 138
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 5 cut(s) 57, 129, 332, 423, 507
PpuMI RGGWCCY 1 cut(s) 271
Psp124BI GAGCTC 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 271
Psp6I CCWGG 1 cut(s) 267
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 2 cut(s) 169, 273
PspPI GGNCC 2 cut(s) 134, 271
PspPPI RGGWCCY 1 cut(s) 271
RigI GGCCGGCC 1 cut(s) 140
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
SacI GAGCTC 1 cut(s) 315
SaqAI TTAA 1 cut(s) 410
SatI GCNGC 5 cut(s) 56, 128, 331, 422, 506
Sau3AI GATC 1 cut(s) 334
Sau96I GGNCC 2 cut(s) 134, 271
ScrFI CCNGG 1 cut(s) 269
SduI GDGCHC 2 cut(s) 170, 315
SetI ASST 7 cut(s) 11, 45, 78, 111, 273, 315, 379
SinI GGWCC 1 cut(s) 271
SpeI ACTAGT 1 cut(s) 359
Sse9I AATT 2 cut(s) 238, 530
SsiI CCGC 4 cut(s) 34, 87, 112, 306
SspMI CTAG 3 cut(s) 12, 360, 365
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 543
TaiI ACGT 2 cut(s) 78, 379
TaqI TCGA 3 cut(s) 252, 315, 339
TasI AATT 2 cut(s) 238, 530
TatI WGTACW 1 cut(s) 21
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 1 cut(s) 410
Tru9I TTAA 1 cut(s) 410
TseI GCWGC 5 cut(s) 55, 127, 330, 421, 505
TspDTI ATGAA 2 cut(s) 419, 469
TspGWI ACGGA 1 cut(s) 160
VpaK11BI GGWCC 1 cut(s) 271
XbaI TCTAGA 1 cut(s) 11
XspI CTAG 3 cut(s) 12, 360, 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.