RchiOBHm_Chr6g0293791

Ribosome biogenesis protein Nop16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009327
Physical Location & Seq
Forward (+)
56317788 .. 56319753
1966 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26363

Sequence Viewer

Length: 570 bp
ATGGGAGGGTCAAGAAGAAAGTACAAGCGGTCTCGTACCAAGGTCCGGGTGGCGCTGCCGAAGCGGAAGCCCAACGTGTTCAAGCCGGCCTTCTGTCTCCCTCCCAAGCTCCGCGCCTTGATCGACCAAGCAGAACCCGCTTCTCGCCCTAAGTGGGACCCCGAGGCCAGCGTCATCCAAAACTACAAGTCGTTCGGCGTCCTCTCCAACCCTAACTTGCTCAACGTCCGCTCTCACATCGTCGAAAGCGACTCTCTCCGGGCCCCTCCCGATCAGGCTGAGGCCGCCGAGCTCGACCCCAACGACGACGGTAGCGATCTCGAAGAAGACGATTTGAAAACTACTCTAGGAAAGAAACGGAGAGATGGGAGACCTGCATCGTTACTACCGTTAACCACCATCCAACGTGTTCATGTCAGTCGGCTTATTGAGAAATATGGAGATGACTATCAGAGGATGTTCATGGATACAAAACTAAATGCAATGCAGCATTCTGTTGCAACTTTGGAGAAGTTATGCAGAAGGTATCACATGTATAAAGATAAAAATCCCCTGATATTGACGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nop16

Protein Analysis

189

Amino Acids

21.67

Weight (kDa)

9.82

Isoelectric Point (pI)

54.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop16 PF09420 107 - 177 2.4e-15 Ribosome biogenesis protein Nop16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 382
AccBSI CCGCTC 1 cut(s) 231
AccII CGCG 2 cut(s) 114, 565
AciI CCGC 6 cut(s) 28, 64, 112, 138, 229, 285
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 2 cut(s) 23, 37
AfiI CCNNNNNNNGG 2 cut(s) 45, 154
AflIII ACRYGT 4 cut(s) 75, 406, 531, 563
AgsI TTSAA 2 cut(s) 82, 337
AluBI AGCT 2 cut(s) 109, 292
AluI AGCT 2 cut(s) 109, 292
Alw21I GWGCWC 1 cut(s) 294
Alw26I GTCTC 3 cut(s) 36, 101, 364
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 4 cut(s) 87, 165, 261, 282
ApaI GGGCCC 1 cut(s) 265
ApeKI GCWGC 2 cut(s) 55, 487
AspLEI GCGC 2 cut(s) 55, 116
AspS9I GGNCC 4 cut(s) 43, 157, 261, 262
AsuC2I CCSGG 2 cut(s) 47, 260
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 2 cut(s) 43, 157
BaeGI GKGCMC 1 cut(s) 265
BanII GRGCYC 2 cut(s) 265, 294
BbsI GAAGAC 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 294
BbvCI CCTCAGC 1 cut(s) 279
BbvI GCAGC 2 cut(s) 42, 499
BccI CCATC 2 cut(s) 359, 407
BciVI GTATCC 1 cut(s) 460
BcnI CCSGG 2 cut(s) 47, 260
BcoDI GTCTC 3 cut(s) 36, 101, 364
BfaI CTAG 1 cut(s) 347
BfoI RGCGCY 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 382
BfuI GTATCC 1 cut(s) 460
BisI GCNGC 3 cut(s) 56, 285, 488
BlsI GCNGC 3 cut(s) 57, 286, 489
Bme1390I CCNGG 2 cut(s) 47, 260
Bme18I GGWCC 2 cut(s) 43, 157
BmeT110I CYCGRG 1 cut(s) 161
BmgT120I GGNCC 4 cut(s) 43, 157, 261, 262
BmiI GGNNCC 4 cut(s) 158, 159, 263, 264
BmrFI CCNGG 2 cut(s) 47, 260
BmsI GCATC 1 cut(s) 386
BpiI GAAGAC 1 cut(s) 333
Bpu10I CCTNAGC 1 cut(s) 279
BpuMI CCSGG 2 cut(s) 47, 260
BsaBI GATNNNNATC 2 cut(s) 447, 546
BsaHI GRCGYC 1 cut(s) 198
BsaI GGTCTC 2 cut(s) 36, 364
BsaJI CCNNGG 2 cut(s) 39, 162
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 154
Bse118I RCCGGY 1 cut(s) 85
Bse3DI GCAATG 1 cut(s) 489
Bse8I GATNNNNATC 2 cut(s) 447, 546
BseDI CCNNGG 2 cut(s) 39, 162
BseGI GGATG 3 cut(s) 174, 399, 462
BseJI GATNNNNATC 2 cut(s) 447, 546
BseLI CCNNNNNNNGG 2 cut(s) 45, 154
BseMI GCAATG 1 cut(s) 489
BseMII CTCAG 1 cut(s) 270
BseSI GKGCMC 1 cut(s) 265
BseXI GCAGC 2 cut(s) 42, 499
Bsh1236I CGCG 2 cut(s) 114, 565
BshFI GGCC 4 cut(s) 89, 167, 263, 284
BsiHKAI GWGCWC 1 cut(s) 294
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 3 cut(s) 46, 86, 259
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 2 cut(s) 45, 154
BsmAI GTCTC 3 cut(s) 36, 101, 364
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 4 cut(s) 89, 167, 263, 284
Bso31I GGTCTC 2 cut(s) 36, 364
BsoBI CYCGRG 1 cut(s) 161
Bsp120I GGGCCC 1 cut(s) 261
Bsp1286I GDGCHC 2 cut(s) 265, 294
Bsp143I GATC 3 cut(s) 120, 271, 316
BspACI CCGC 6 cut(s) 28, 64, 112, 138, 229, 285
BspANI GGCC 4 cut(s) 89, 167, 263, 284
BspCNI CTCAG 1 cut(s) 271
BspFNI CGCG 2 cut(s) 114, 565
BspLI GGNNCC 4 cut(s) 158, 159, 263, 264
BspMI ACCTGC 1 cut(s) 382
BspTNI GGTCTC 2 cut(s) 36, 364
BsrBI CCGCTC 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 489
BsrFI RCCGGY 1 cut(s) 85
BssAI RCCGGY 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 39, 162
BssMI GATC 3 cut(s) 120, 271, 316
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 39
Bst4CI ACNGT 2 cut(s) 311, 390
BstACI GRCGYC 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 87, 169
BstDEI CTNAG 2 cut(s) 150, 279
BstF5I GGATG 3 cut(s) 174, 399, 462
BstFNI CGCG 2 cut(s) 114, 565
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 2 cut(s) 55, 116
BstKTI GATC 3 cut(s) 123, 274, 319
BstMAI GTCTC 3 cut(s) 36, 101, 364
BstMBI GATC 3 cut(s) 120, 271, 316
BstMWI GCNNNNNNNGC 3 cut(s) 61, 137, 284
BstNSI RCATGY 1 cut(s) 535
BstSCI CCNGG 2 cut(s) 45, 258
BstSLI GKGCMC 1 cut(s) 265
BstUI CGCG 2 cut(s) 114, 565
BstV1I GCAGC 2 cut(s) 42, 499
BstV2I GAAGAC 1 cut(s) 333
BsuI GTATCC 1 cut(s) 460
BsuRI GGCC 4 cut(s) 89, 167, 263, 284
BtsCI GGATG 3 cut(s) 174, 399, 462
BveI ACCTGC 1 cut(s) 382
Cac8I GCNNGC 2 cut(s) 87, 169
CfoI GCGC 2 cut(s) 55, 116
Cfr10I RCCGGY 1 cut(s) 85
Cfr13I GGNCC 4 cut(s) 43, 157, 261, 262
CseI GACGC 2 cut(s) 160, 187
Csp6I GTAC 2 cut(s) 22, 36
CviAII CATG 3 cut(s) 413, 463, 532
CviQI GTAC 2 cut(s) 22, 36
DdeI CTNAG 2 cut(s) 150, 279
DpnI GATC 3 cut(s) 122, 273, 318
DpnII GATC 3 cut(s) 120, 271, 316
Ecl136II GAGCTC 1 cut(s) 292
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 2 cut(s) 265, 294
Eco31I GGTCTC 2 cut(s) 36, 364
Eco47I GGWCC 2 cut(s) 43, 157
Eco53kI GAGCTC 1 cut(s) 292
Eco88I CYCGRG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 292
EcoO109I RGGNCCY 2 cut(s) 157, 262
EcoT14I CCWWGG 1 cut(s) 39
EcoT38I GRGCYC 2 cut(s) 265, 294
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 3 cut(s) 416, 466, 535
FaiI YATR 6 cut(s) 414, 438, 464, 517, 533, 537
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 1 cut(s) 170
FatI CATG 3 cut(s) 412, 462, 531
FauI CCCGC 1 cut(s) 145
Fnu4HI GCNGC 3 cut(s) 56, 285, 488
FokI GGATG 3 cut(s) 161, 386, 469
FriOI GRGCYC 2 cut(s) 265, 294
Fsp4HI GCNGC 3 cut(s) 56, 285, 488
FspBI CTAG 1 cut(s) 347
GlaI GCGC 2 cut(s) 54, 115
GluI GCNGC 3 cut(s) 56, 285, 488
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 4 cut(s) 89, 167, 263, 284
HapII CCGG 3 cut(s) 46, 86, 259
HgaI GACGC 2 cut(s) 160, 187
HhaI GCGC 2 cut(s) 55, 116
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 3 cut(s) 416, 466, 535
Hin6I GCGC 2 cut(s) 53, 114
HinP1I GCGC 2 cut(s) 53, 114
HincII GTYRAC 1 cut(s) 393
HindII GTYRAC 1 cut(s) 393
HinfI GANTC 1 cut(s) 251
HpaI GTTAAC 1 cut(s) 393
HpaII CCGG 3 cut(s) 46, 86, 259
Hpy166II GTNNAC 1 cut(s) 393
Hpy188I TCNGA 1 cut(s) 453
Hpy188III TCNNGA 3 cut(s) 12, 269, 320
Hpy8I GTNNAC 1 cut(s) 393
Hpy99I CGWCG 3 cut(s) 245, 308, 311
HpyAV CCTTC 2 cut(s) 100, 516
HpyCH4III ACNGT 2 cut(s) 311, 390
HpyCH4IV ACGT 3 cut(s) 75, 225, 406
HpyCH4V TGCA 5 cut(s) 377, 482, 487, 500, 519
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 137, 284
HpyF3I CTNAG 2 cut(s) 150, 279
HpySE526I ACGT 3 cut(s) 75, 225, 406
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 3 cut(s) 416, 466, 535
HspAI GCGC 2 cut(s) 53, 114
KflI GGGWCCC 1 cut(s) 157
KroI GCCGGC 1 cut(s) 85
KroNI GCCGGC 1 cut(s) 87
KspAI GTTAAC 1 cut(s) 393
Kzo9I GATC 3 cut(s) 120, 271, 316
LmnI GCTCC 1 cut(s) 114
LpnPI CCDG 7 cut(s) 59, 99, 181, 260, 272, 387, 566
Lsp1109I GCAGC 2 cut(s) 42, 499
LweI GCATC 1 cut(s) 386
MaeI CTAG 1 cut(s) 347
MaeII ACGT 3 cut(s) 75, 225, 406
MaeIII GTNAC 1 cut(s) 381
MalI GATC 3 cut(s) 122, 273, 318
MbiI CCGCTC 1 cut(s) 231
MboI GATC 3 cut(s) 120, 271, 316
MboII GAAGA 3 cut(s) 27, 335, 338
MhlI GDGCHC 2 cut(s) 265, 294
MluI ACGCGT 1 cut(s) 563
MlyI GAGTC 1 cut(s) 245
MmeI TCCRAC 2 cut(s) 231, 427
MnlI CCTC 6 cut(s) 111, 157, 212, 274, 276, 447
MroNI GCCGGC 1 cut(s) 85
MseI TTAA 1 cut(s) 392
MspI CCGG 3 cut(s) 46, 86, 259
MspR9I CCNGG 2 cut(s) 47, 260
Mva1269I GAATGC 1 cut(s) 490
MvnI CGCG 2 cut(s) 114, 565
MwoI GCNNNNNNNGC 3 cut(s) 61, 137, 284
NaeI GCCGGC 1 cut(s) 87
NciI CCSGG 2 cut(s) 47, 260
NdeII GATC 3 cut(s) 120, 271, 316
NgoMIV GCCGGC 1 cut(s) 85
NlaIII CATG 3 cut(s) 416, 466, 535
NlaIV GGNNCC 4 cut(s) 158, 159, 263, 264
NmeAIII GCCGAG 1 cut(s) 313
NspI RCATGY 1 cut(s) 535
PciI ACATGT 1 cut(s) 531
PcsI WCGNNNNNNNCGW 5 cut(s) 246, 300, 312, 327, 386
PctI GAATGC 1 cut(s) 490
PdiI GCCGGC 1 cut(s) 87
PkrI GCNGC 3 cut(s) 57, 286, 489
PleI GAGTC 1 cut(s) 245
PpsI GAGTC 1 cut(s) 245
PpuMI RGGWCCY 1 cut(s) 157
PscI ACATGT 1 cut(s) 531
Psp124BI GAGCTC 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 157
PspN4I GGNNCC 4 cut(s) 158, 159, 263, 264
PspOMI GGGCCC 1 cut(s) 261
PspPI GGNCC 4 cut(s) 43, 157, 261, 262
PspPPI RGGWCCY 1 cut(s) 157
RsaI GTAC 2 cut(s) 23, 37
RsaNI GTAC 2 cut(s) 22, 36
SacI GAGCTC 1 cut(s) 294
SaqAI TTAA 1 cut(s) 392
SatI GCNGC 3 cut(s) 56, 285, 488
Sau3AI GATC 3 cut(s) 120, 271, 316
Sau96I GGNCC 4 cut(s) 43, 157, 261, 262
SchI GAGTC 1 cut(s) 245
ScrFI CCNGG 2 cut(s) 47, 260
SduI GDGCHC 2 cut(s) 265, 294
SetI ASST 8 cut(s) 45, 78, 111, 228, 294, 376, 409, 527
SfaNI GCATC 1 cut(s) 386
SinI GGWCC 2 cut(s) 43, 157
SsiI CCGC 6 cut(s) 28, 64, 112, 138, 229, 285
SspMI CTAG 1 cut(s) 347
SstI GAGCTC 1 cut(s) 294
StyD4I CCNGG 2 cut(s) 45, 258
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 2 cut(s) 311, 390
TaiI ACGT 3 cut(s) 78, 228, 409
TaqI TCGA 4 cut(s) 123, 243, 294, 321
TatI WGTACW 1 cut(s) 21
TauI GCSGC 1 cut(s) 287
Tru1I TTAA 1 cut(s) 392
Tru9I TTAA 1 cut(s) 392
TseI GCWGC 2 cut(s) 55, 487
TspDTI ATGAA 2 cut(s) 401, 451
TspGWI ACGGA 1 cut(s) 373
VpaK11BI GGWCC 2 cut(s) 43, 157
XceI RCATGY 1 cut(s) 535
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XspI CTAG 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.