Prupe.8G259900_v2.0.a1

Ribosome biogenesis protein Nop16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0009327
Physical Location & Seq
Forward (+)
22008502 .. 22010675
2174 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G259900.1

Sequence Viewer

Length: 564 bp
ATGGGAAGGTCACGAAGAAAGTACAAGCAATCGAGGACGAAGGTCCGCGTGGCGCTGCCGAAGAGGAAGCCCAACGTCTTCAAGGCAGCCTTCTGTCTACCTCCGAAGCTTCGCGCCTTACTGGAACAACAAGGCCCATCCTCTGCCTCATGGGACGCCGAGGGCACTGTTATCGAAAACTATAAGTCCTTCGGCTTCGTTTCTAACCCTAACTTGCTCGGCGTCCGCTCTCGAACCTCTCACATGATCGAAAGCGATTCTCTCCAGGTCCCTCCTCCGCCTCCTTCGGGAGATGAGCCCGTCGACGGTAGCGACGTCGAAGAAGACGATTTGAGATCTAGTCTAGGGAAAAAGCGGAGAGATGGAAAATCTGCACCACTGCAACCATTAACGGCCATGCAACGCGTTTATATTGGTCGGCTCATTGAGAAGTATGGAGACGACTATCAGGGAATGTTCATGGATACAAAACTAAATGCAATGCAGCATTCTGTTGCAACTTTGGAGAAACTATGCAAAAGGTATCACGTGCATAAAGATAAAAATCCCTTATTGATCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nop16

Protein Analysis

188

Amino Acids

21.12

Weight (kDa)

9.82

Isoelectric Point (pI)

64.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 318
AccBSI CCGCTC 1 cut(s) 228
AccI GTMKAC 2 cut(s) 97, 303
AccII CGCG 3 cut(s) 48, 114, 405
AciI CCGC 4 cut(s) 46, 226, 278, 355
AclWI GGATC 1 cut(s) 550
AcoI YGGCCR 1 cut(s) 393
AcvI CACGTG 1 cut(s) 529
AcyI GRCGYC 3 cut(s) 156, 222, 315
AfaI GTAC 1 cut(s) 23
AfiI CCNNNNNNNGG 2 cut(s) 287, 305
AflIII ACRYGT 1 cut(s) 403
AgsI TTSAA 1 cut(s) 82
AjnI CCWGG 1 cut(s) 264
AluBI AGCT 1 cut(s) 109
AluI AGCT 1 cut(s) 109
Alw26I GTCTC 1 cut(s) 432
AlwI GGATC 1 cut(s) 550
AoxI GGCC 2 cut(s) 133, 393
ApeKI GCWGC 3 cut(s) 55, 86, 484
AspLEI GCGC 2 cut(s) 55, 116
AspS9I GGNCC 3 cut(s) 43, 134, 268
AvaII GGWCC 2 cut(s) 43, 268
BaeGI GKGCMC 1 cut(s) 167
BanII GRGCYC 1 cut(s) 300
BbrPI CACGTG 1 cut(s) 529
BbsI GAAGAC 2 cut(s) 70, 330
BbvI GCAGC 3 cut(s) 42, 98, 496
BccI CCATC 2 cut(s) 145, 356
BceAI ACGGC 1 cut(s) 408
BcgI CGANNNNNNTGC 2 cut(s) 154, 188
BciT130I CCWGG 1 cut(s) 266
BciVI GTATCC 1 cut(s) 457
BcoDI GTCTC 1 cut(s) 432
BfaI CTAG 2 cut(s) 339, 344
BfoI RGCGCY 1 cut(s) 56
BfuI GTATCC 1 cut(s) 457
BglII AGATCT 1 cut(s) 335
BisI GCNGC 3 cut(s) 56, 87, 485
BlsI GCNGC 3 cut(s) 57, 88, 486
Bme1390I CCNGG 1 cut(s) 266
Bme18I GGWCC 2 cut(s) 43, 268
BmgT120I GGNCC 3 cut(s) 43, 134, 268
BmiI GGNNCC 1 cut(s) 270
BmrFI CCNGG 1 cut(s) 266
BoxI GACNNNNGTC 1 cut(s) 41
BpiI GAAGAC 2 cut(s) 70, 330
BpmI CTGGAG 1 cut(s) 248
BsaAI YACGTR 1 cut(s) 529
BsaBI GATNNNNATC 1 cut(s) 543
BsaHI GRCGYC 3 cut(s) 156, 222, 315
BsaJI CCNNGG 1 cut(s) 159
Bsc4I CCNNNNNNNGG 2 cut(s) 287, 305
Bse1I ACTGG 1 cut(s) 126
Bse3DI GCAATG 1 cut(s) 486
Bse8I GATNNNNATC 1 cut(s) 543
BseBI CCWGG 1 cut(s) 266
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 1 cut(s) 137
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 2 cut(s) 287, 305
BseMI GCAATG 1 cut(s) 486
BseNI ACTGG 1 cut(s) 126
BseRI GAGGAG 1 cut(s) 264
BseSI GKGCMC 1 cut(s) 167
BseXI GCAGC 3 cut(s) 42, 98, 496
BsgI GTGCAG 1 cut(s) 357
Bsh1236I CGCG 3 cut(s) 48, 114, 405
BshFI GGCC 2 cut(s) 135, 395
BslFI GGGAC 2 cut(s) 167, 254
BslI CCNNNNNNNGG 2 cut(s) 287, 305
BsmAI GTCTC 1 cut(s) 432
BsmBI CGTCTC 1 cut(s) 432
BsmFI GGGAC 2 cut(s) 167, 254
BsmI GAATGC 1 cut(s) 487
BsnI GGCC 2 cut(s) 135, 395
Bsp1286I GDGCHC 2 cut(s) 167, 300
Bsp143I GATC 3 cut(s) 246, 335, 555
BspACI CCGC 4 cut(s) 46, 226, 278, 355
BspANI GGCC 2 cut(s) 135, 395
BspFNI CGCG 3 cut(s) 48, 114, 405
BspLI GGNNCC 1 cut(s) 270
BspPI GGATC 1 cut(s) 550
BsrBI CCGCTC 1 cut(s) 228
BsrDI GCAATG 1 cut(s) 486
BsrI ACTGG 1 cut(s) 126
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 3 cut(s) 246, 335, 555
BssNI GRCGYC 3 cut(s) 156, 222, 315
Bst2UI CCWGG 1 cut(s) 266
Bst4CI ACNGT 2 cut(s) 169, 308
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 3 cut(s) 156, 222, 315
BstBAI YACGTR 1 cut(s) 529
BstF5I GGATG 1 cut(s) 137
BstFNI CGCG 3 cut(s) 48, 114, 405
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 2 cut(s) 55, 116
BstKTI GATC 3 cut(s) 249, 338, 558
BstMAI GTCTC 1 cut(s) 432
BstMBI GATC 3 cut(s) 246, 335, 555
BstNI CCWGG 1 cut(s) 266
BstPAI GACNNNNGTC 1 cut(s) 41
BstSCI CCNGG 1 cut(s) 264
BstSLI GKGCMC 1 cut(s) 167
BstUI CGCG 3 cut(s) 48, 114, 405
BstV1I GCAGC 3 cut(s) 42, 98, 496
BstV2I GAAGAC 2 cut(s) 70, 330
BstX2I RGATCY 1 cut(s) 335
BstYI RGATCY 1 cut(s) 335
BsuI GTATCC 1 cut(s) 457
BsuRI GGCC 2 cut(s) 135, 395
BtsCI GGATG 1 cut(s) 137
BtsI GCAGTG 1 cut(s) 377
BtsIMutI CAGTG 2 cut(s) 165, 377
CfoI GCGC 2 cut(s) 55, 116
Cfr13I GGNCC 3 cut(s) 43, 134, 268
CseI GACGC 2 cut(s) 164, 211
Csp6I GTAC 1 cut(s) 22
CviAII CATG 4 cut(s) 150, 244, 397, 460
CviJI RGCY 8 cut(s) 70, 89, 109, 135, 195, 298, 395, 421
CviKI_1 RGCY 8 cut(s) 70, 89, 109, 135, 195, 298, 395, 421
CviQI GTAC 1 cut(s) 22
DpnI GATC 3 cut(s) 248, 337, 557
DpnII GATC 3 cut(s) 246, 335, 555
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
EciI GGCGGA 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 300
Eco47I GGWCC 2 cut(s) 43, 268
Eco72I CACGTG 1 cut(s) 529
EcoO109I RGGNCCY 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 300
Esp3I CGTCTC 1 cut(s) 432
FaeI CATG 4 cut(s) 153, 247, 400, 463
FaiI YATR 9 cut(s) 151, 183, 245, 398, 411, 435, 461, 514, 534
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 2 cut(s) 167, 254
FatI CATG 4 cut(s) 149, 243, 396, 459
FblI GTMKAC 2 cut(s) 97, 303
Fnu4HI GCNGC 3 cut(s) 56, 87, 485
FokI GGATG 1 cut(s) 124
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 3 cut(s) 56, 87, 485
FspBI CTAG 2 cut(s) 339, 344
GlaI GCGC 2 cut(s) 54, 115
GluI GCNGC 3 cut(s) 56, 87, 485
GsuI CTGGAG 1 cut(s) 248
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 2 cut(s) 135, 395
HgaI GACGC 2 cut(s) 164, 211
HhaI GCGC 2 cut(s) 55, 116
Hin1I GRCGYC 3 cut(s) 156, 222, 315
Hin1II CATG 4 cut(s) 153, 247, 400, 463
Hin6I GCGC 2 cut(s) 53, 114
HinP1I GCGC 2 cut(s) 53, 114
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 1 cut(s) 257
Hpy166II GTNNAC 2 cut(s) 98, 304
Hpy188I TCNGA 1 cut(s) 105
Hpy188III TCNNGA 3 cut(s) 12, 231, 288
Hpy8I GTNNAC 2 cut(s) 98, 304
Hpy99I CGWCG 4 cut(s) 305, 308, 317, 320
HpyAV CCTTC 4 cut(s) 34, 100, 199, 294
HpyCH4III ACNGT 2 cut(s) 169, 308
HpyCH4IV ACGT 3 cut(s) 75, 315, 528
HpyCH4V TGCA 8 cut(s) 374, 382, 400, 479, 484, 497, 516, 532
HpySE526I ACGT 3 cut(s) 75, 315, 528
Hsp92I GRCGYC 3 cut(s) 156, 222, 315
Hsp92II CATG 4 cut(s) 153, 247, 400, 463
HspAI GCGC 2 cut(s) 53, 114
Kzo9I GATC 3 cut(s) 246, 335, 555
LpnPI CCDG 4 cut(s) 107, 251, 278, 434
Lsp1109I GCAGC 3 cut(s) 42, 98, 496
MaeI CTAG 2 cut(s) 339, 344
MaeII ACGT 3 cut(s) 75, 315, 528
MaeIII GTNAC 1 cut(s) 9
MalI GATC 3 cut(s) 248, 337, 557
MbiI CCGCTC 1 cut(s) 228
MboI GATC 3 cut(s) 246, 335, 555
MboII GAAGA 5 cut(s) 27, 70, 73, 332, 335
MflI RGATCY 1 cut(s) 335
MhlI GDGCHC 2 cut(s) 167, 300
MluI ACGCGT 1 cut(s) 403
MseI TTAA 1 cut(s) 389
MspR9I CCNGG 1 cut(s) 266
Mva1269I GAATGC 1 cut(s) 487
MvaI CCWGG 1 cut(s) 266
MvnI CGCG 3 cut(s) 48, 114, 405
NdeII GATC 3 cut(s) 246, 335, 555
NlaIII CATG 4 cut(s) 153, 247, 400, 463
NlaIV GGNNCC 1 cut(s) 270
NmeAIII GCCGAG 2 cut(s) 184, 198
NmuCI GTSAC 1 cut(s) 9
PcsI WCGNNNNNNNCGW 3 cut(s) 309, 312, 324
PctI GAATGC 1 cut(s) 487
PfeI GAWTC 1 cut(s) 257
PkrI GCNGC 3 cut(s) 57, 88, 486
PmaCI CACGTG 1 cut(s) 529
PmlI CACGTG 1 cut(s) 529
Ppu21I YACGTR 1 cut(s) 529
PpuMI RGGWCCY 1 cut(s) 268
PshAI GACNNNNGTC 1 cut(s) 41
Psp5II RGGWCCY 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 264
PspCI CACGTG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 264
PspN4I GGNNCC 1 cut(s) 270
PspPI GGNCC 3 cut(s) 43, 134, 268
PspPPI RGGWCCY 1 cut(s) 268
PsuI RGATCY 1 cut(s) 335
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
SalI GTCGAC 1 cut(s) 302
SaqAI TTAA 1 cut(s) 389
SatI GCNGC 3 cut(s) 56, 87, 485
Sau3AI GATC 3 cut(s) 246, 335, 555
Sau96I GGNCC 3 cut(s) 43, 134, 268
ScrFI CCNGG 1 cut(s) 266
SduI GDGCHC 2 cut(s) 167, 300
SgrDI CGTCGACG 1 cut(s) 302
SinI GGWCC 2 cut(s) 43, 268
SsiI CCGC 4 cut(s) 46, 226, 278, 355
SspMI CTAG 2 cut(s) 339, 344
StyD4I CCNGG 1 cut(s) 264
TaaI ACNGT 2 cut(s) 169, 308
TaiI ACGT 3 cut(s) 78, 318, 531
TaqI TCGA 6 cut(s) 32, 174, 232, 249, 303, 318
TatI WGTACW 1 cut(s) 21
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 1 cut(s) 389
Tru9I TTAA 1 cut(s) 389
TscAI CASTG 2 cut(s) 172, 384
TseFI GTSAC 1 cut(s) 9
TseI GCWGC 3 cut(s) 55, 86, 484
Tsp45I GTSAC 1 cut(s) 9
TspDTI ATGAA 1 cut(s) 448
TspGWI ACGGA 1 cut(s) 548
TspRI CASTG 2 cut(s) 172, 384
VpaK11BI GGWCC 2 cut(s) 43, 268
XmiI GTMKAC 2 cut(s) 97, 303
XspI CTAG 2 cut(s) 339, 344
ZraI GACGTC 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.