pycom03g22710

Ribosome biogenesis protein Nop16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009327
Physical Location & Seq
Forward (+)
22770498 .. 22774151
3654 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g22710.2

Sequence Viewer

Length: 588 bp
ATGGGAAGGTCACGAAGAAAGTACAAGCAGTCGCGGACGAAGGTGCGCGTGGGGCTGCCGAAAAAGAAGCCCAACGTCTTCAAGCCCGCTTTCTGCCTCCCTCCGAAGCTGCGCGCCTTGCTAGAGCAGCAAGGCCCGGCCTCCGCCCCCCAATGGGACGCCGAGGGCTCCGTCATCCAAAACTACAGGTCCTTCGGCTTCGTCTCCAACCCTAACTTGCTCAGCCTCCGAGCTCGCAACTCTCACGTGGTCGAGAGCGATTCTCTCCAGGTTCCCCTACCTCCCTCCGATGACCCTTACGCCGCCGCCGAGCTCGATTTTGGCGACGGTAGCGACCTCGAAGAGGACGATTTGAAAACTAGTCTAGGGAAGAAACGGAGGGACGGAAAATCTGCACCACTACAACCATTAACAGCCATGCAGCGTCTTCATATTGGTCGGCTCATTGAGAAGTATGGCGATGACTATCAGAGAATGTTCATGGACACAAAACTAAATGCAATGCAGCATTCCGTTGCAACTTTGGAGAAATTATGCAAACGATATCATGTGCATAAAGATCGAAATCCCTTGATATCGACCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nop16

Protein Analysis

196

Amino Acids

22.11

Weight (kDa)

9.82

Isoelectric Point (pI)

53.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 34, 48, 114
AciI CCGC 5 cut(s) 34, 87, 144, 303, 306
AcvI CACGTG 1 cut(s) 247
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 1 cut(s) 23
AfiI CCNNNNNNNGG 3 cut(s) 153, 154, 343
AgsI TTSAA 2 cut(s) 82, 355
AhlI ACTAGT 1 cut(s) 359
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 3 cut(s) 109, 233, 313
AluI AGCT 3 cut(s) 109, 233, 313
Alw21I GWGCWC 2 cut(s) 235, 315
Alw26I GTCTC 1 cut(s) 208
AoxI GGCC 2 cut(s) 133, 138
ApeKI GCWGC 5 cut(s) 55, 109, 127, 421, 505
AspLEI GCGC 3 cut(s) 48, 114, 116
AspS9I GGNCC 2 cut(s) 134, 189
AsuC2I CCSGG 1 cut(s) 137
AvaII GGWCC 1 cut(s) 189
BanII GRGCYC 3 cut(s) 170, 235, 315
BbrPI CACGTG 1 cut(s) 247
BbsI GAAGAC 2 cut(s) 70, 419
Bbv12I GWGCWC 2 cut(s) 235, 315
BbvI GCAGC 5 cut(s) 42, 96, 139, 433, 517
BcgI CGANNNNNNTGC 2 cut(s) 542, 576
BciT130I CCWGG 1 cut(s) 269
BcnI CCSGG 1 cut(s) 137
BcoDI GTCTC 1 cut(s) 208
BcuI ACTAGT 1 cut(s) 359
BfaI CTAG 3 cut(s) 122, 360, 365
BfmI CTRYAG 1 cut(s) 184
BisI GCNGC 7 cut(s) 56, 110, 128, 303, 306, 422, 506
BlpI GCTNAGC 1 cut(s) 221
BlsI GCNGC 7 cut(s) 57, 111, 129, 304, 307, 423, 507
Bme1390I CCNGG 2 cut(s) 137, 269
Bme18I GGWCC 1 cut(s) 189
BmgT120I GGNCC 2 cut(s) 134, 189
BmiI GGNNCC 2 cut(s) 169, 273
BmrFI CCNGG 2 cut(s) 137, 269
BpiI GAAGAC 2 cut(s) 70, 419
BplI GAGNNNNNCTC 2 cut(s) 247, 279
BpmI CTGGAG 1 cut(s) 251
Bpu1102I GCTNAGC 1 cut(s) 221
BpuMI CCSGG 1 cut(s) 137
BsaAI YACGTR 1 cut(s) 247
BsaBI GATNNNNATC 2 cut(s) 465, 564
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 1 cut(s) 162
Bsc4I CCNNNNNNNGG 3 cut(s) 153, 154, 343
Bse3DI GCAATG 1 cut(s) 507
Bse8I GATNNNNATC 2 cut(s) 465, 564
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 1 cut(s) 162
BseGI GGATG 1 cut(s) 174
BseJI GATNNNNATC 2 cut(s) 465, 564
BseLI CCNNNNNNNGG 3 cut(s) 153, 154, 343
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 1 cut(s) 235
BsePI GCGCGC 1 cut(s) 112
BseXI GCAGC 5 cut(s) 42, 96, 139, 433, 517
BsgI GTGCAG 1 cut(s) 378
Bsh1236I CGCG 3 cut(s) 34, 48, 114
BshFI GGCC 2 cut(s) 135, 140
BsiHKAI GWGCWC 2 cut(s) 235, 315
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 2 cut(s) 170, 395
BslI CCNNNNNNNGG 3 cut(s) 153, 154, 343
BsmAI GTCTC 1 cut(s) 208
BsmBI CGTCTC 1 cut(s) 208
BsmFI GGGAC 2 cut(s) 170, 395
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 2 cut(s) 135, 140
Bsp1286I GDGCHC 3 cut(s) 170, 235, 315
Bsp143I GATC 1 cut(s) 559
Bsp1720I GCTNAGC 1 cut(s) 221
BspACI CCGC 5 cut(s) 34, 87, 144, 303, 306
BspANI GGCC 2 cut(s) 135, 140
BspCNI CTCAG 1 cut(s) 234
BspFNI CGCG 3 cut(s) 34, 48, 114
BspLI GGNNCC 2 cut(s) 169, 273
BsrDI GCAATG 1 cut(s) 507
BssECI CCNNGG 1 cut(s) 162
BssHII GCGCGC 1 cut(s) 112
BssMI GATC 1 cut(s) 559
BssNI GRCGYC 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 1 cut(s) 329
Bst6I CTCTTC 1 cut(s) 336
BstACI GRCGYC 1 cut(s) 159
BstBAI YACGTR 1 cut(s) 247
BstC8I GCNNGC 3 cut(s) 87, 114, 235
BstDEI CTNAG 1 cut(s) 221
BstENI CCTNNNNNAGG 1 cut(s) 341
BstF5I GGATG 1 cut(s) 174
BstFNI CGCG 3 cut(s) 34, 48, 114
BstHHI GCGC 3 cut(s) 48, 114, 116
BstKTI GATC 1 cut(s) 562
BstMAI GTCTC 1 cut(s) 208
BstMBI GATC 1 cut(s) 559
BstMWI GCNNNNNNNGC 4 cut(s) 52, 118, 127, 330
BstNI CCWGG 1 cut(s) 269
BstSCI CCNGG 2 cut(s) 135, 267
BstSFI CTRYAG 1 cut(s) 184
BstUI CGCG 3 cut(s) 34, 48, 114
BstV1I GCAGC 5 cut(s) 42, 96, 139, 433, 517
BstV2I GAAGAC 2 cut(s) 70, 419
BsuRI GGCC 2 cut(s) 135, 140
BtgZI GCGATG 1 cut(s) 474
BtsCI GGATG 1 cut(s) 174
Cac8I GCNNGC 3 cut(s) 87, 114, 235
CfoI GCGC 3 cut(s) 48, 114, 116
Cfr13I GGNCC 2 cut(s) 134, 189
CseI GACGC 2 cut(s) 167, 413
Csp6I GTAC 1 cut(s) 22
CviAII CATG 3 cut(s) 418, 481, 548
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 1 cut(s) 221
DpnI GATC 1 cut(s) 561
DpnII GATC 1 cut(s) 559
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
EciI GGCGGA 1 cut(s) 133
Ecl136II GAGCTC 2 cut(s) 233, 313
Eco24I GRGCYC 3 cut(s) 170, 235, 315
Eco32I GATATC 2 cut(s) 545, 576
Eco47I GGWCC 1 cut(s) 189
Eco53kI GAGCTC 2 cut(s) 233, 313
Eco72I CACGTG 1 cut(s) 247
EcoICRI GAGCTC 2 cut(s) 233, 313
EcoNI CCTNNNNNAGG 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 189
EcoRII CCWGG 1 cut(s) 267
EcoRV GATATC 2 cut(s) 545, 576
EcoT38I GRGCYC 3 cut(s) 170, 235, 315
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 3 cut(s) 421, 484, 551
FaiI YATR 7 cut(s) 419, 432, 456, 482, 535, 549, 555
FaqI GGGAC 2 cut(s) 170, 395
FatI CATG 3 cut(s) 417, 480, 547
FauI CCCGC 1 cut(s) 94
Fnu4HI GCNGC 7 cut(s) 56, 110, 128, 303, 306, 422, 506
FokI GGATG 1 cut(s) 161
FriOI GRGCYC 3 cut(s) 170, 235, 315
Fsp4HI GCNGC 7 cut(s) 56, 110, 128, 303, 306, 422, 506
FspBI CTAG 3 cut(s) 122, 360, 365
GlaI GCGC 3 cut(s) 47, 113, 115
GluI GCNGC 7 cut(s) 56, 110, 128, 303, 306, 422, 506
GsuI CTGGAG 1 cut(s) 251
HaeIII GGCC 2 cut(s) 135, 140
HapII CCGG 1 cut(s) 137
HgaI GACGC 2 cut(s) 167, 413
HhaI GCGC 3 cut(s) 48, 114, 116
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 3 cut(s) 421, 484, 551
Hin6I GCGC 3 cut(s) 46, 112, 114
HinP1I GCGC 3 cut(s) 46, 112, 114
HinfI GANTC 1 cut(s) 260
HpaII CCGG 1 cut(s) 137
Hpy188I TCNGA 4 cut(s) 105, 230, 289, 471
Hpy188III TCNNGA 2 cut(s) 12, 253
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 2 cut(s) 34, 202
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4IV ACGT 2 cut(s) 75, 246
HpyCH4V TGCA 7 cut(s) 395, 421, 500, 505, 518, 537, 553
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 118, 127, 330
HpyF3I CTNAG 1 cut(s) 221
HpySE526I ACGT 2 cut(s) 75, 246
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 3 cut(s) 421, 484, 551
HspAI GCGC 3 cut(s) 46, 112, 114
Kzo9I GATC 1 cut(s) 559
LmnI GCTCC 1 cut(s) 173
LpnPI CCDG 4 cut(s) 150, 172, 254, 281
Lsp1109I GCAGC 5 cut(s) 42, 96, 139, 433, 517
MaeI CTAG 3 cut(s) 122, 360, 365
MaeII ACGT 2 cut(s) 75, 246
MaeIII GTNAC 1 cut(s) 9
MalI GATC 1 cut(s) 561
MboI GATC 1 cut(s) 559
MboII GAAGA 5 cut(s) 27, 70, 353, 382, 419
MhlI GDGCHC 3 cut(s) 170, 235, 315
MluCI AATT 1 cut(s) 530
MmeI TCCRAC 1 cut(s) 231
MseI TTAA 1 cut(s) 410
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 2 cut(s) 137, 269
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 3 cut(s) 34, 48, 114
MwoI GCNNNNNNNGC 4 cut(s) 52, 118, 127, 330
NciI CCSGG 1 cut(s) 137
NdeII GATC 1 cut(s) 559
NlaIII CATG 3 cut(s) 421, 484, 551
NlaIV GGNNCC 2 cut(s) 169, 273
NmeAIII GCCGAG 2 cut(s) 187, 334
NmuCI GTSAC 1 cut(s) 9
PauI GCGCGC 1 cut(s) 112
PcsI WCGNNNNNNNCGW 3 cut(s) 306, 321, 345
PctI GAATGC 1 cut(s) 508
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 7 cut(s) 57, 111, 129, 304, 307, 423, 507
PmaCI CACGTG 1 cut(s) 247
PmlI CACGTG 1 cut(s) 247
Ppu21I YACGTR 1 cut(s) 247
PpuMI RGGWCCY 1 cut(s) 189
Psp124BI GAGCTC 2 cut(s) 235, 315
Psp5II RGGWCCY 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 267
PspCI CACGTG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 2 cut(s) 169, 273
PspPI GGNCC 2 cut(s) 134, 189
PspPPI RGGWCCY 1 cut(s) 189
PteI GCGCGC 1 cut(s) 112
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
SacI GAGCTC 2 cut(s) 235, 315
SaqAI TTAA 1 cut(s) 410
SatI GCNGC 7 cut(s) 56, 110, 128, 303, 306, 422, 506
Sau3AI GATC 1 cut(s) 559
Sau96I GGNCC 2 cut(s) 134, 189
ScrFI CCNGG 2 cut(s) 137, 269
SduI GDGCHC 3 cut(s) 170, 235, 315
SfcI CTRYAG 1 cut(s) 184
SinI GGWCC 1 cut(s) 189
SpeI ACTAGT 1 cut(s) 359
Sse9I AATT 1 cut(s) 530
SsiI CCGC 5 cut(s) 34, 87, 144, 303, 306
SspMI CTAG 3 cut(s) 122, 360, 365
SstI GAGCTC 2 cut(s) 235, 315
StyD4I CCNGG 2 cut(s) 135, 267
TaaI ACNGT 1 cut(s) 329
TaiI ACGT 2 cut(s) 78, 249
TaqI TCGA 5 cut(s) 252, 315, 339, 562, 578
TasI AATT 1 cut(s) 530
TatI WGTACW 1 cut(s) 21
TauI GCSGC 2 cut(s) 305, 308
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 1 cut(s) 410
Tru9I TTAA 1 cut(s) 410
TseFI GTSAC 1 cut(s) 9
TseI GCWGC 5 cut(s) 55, 109, 127, 421, 505
Tsp45I GTSAC 1 cut(s) 9
TspDTI ATGAA 2 cut(s) 419, 469
TspGWI ACGGA 4 cut(s) 160, 391, 399, 502
VpaK11BI GGWCC 1 cut(s) 189
XagI CCTNNNNNAGG 1 cut(s) 341
XspI CTAG 3 cut(s) 122, 360, 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.