FvH4_2g31800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011853
Physical Location & Seq
Reverse (-)
24293015 .. 24293815
801 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31800.t1

Sequence Viewer

Length: 801 bp
ATGACGGACAGAGTCTACCCGTCTTCAAAGGTCGCCGCCAACGGCGCCACTAACGGCGCTACCGCCACCACCACAGCCGCCAACCCCACGGCCACCAAGCCGCAGCCGCTCCGGCAGCCCTACCGTCCTCAGCCGCAGTACCACCACCGTCGGCACCGCCGAAGCAACCGGAGTCTCTGCTGCTGCTGTTGTTTCTGGTCCATACTTATATTCTTGATTGTGGCTCTCCTCGCCGCTATAGCCGGCACGGCTCTCTACGTCCTCTACCGTCCCCACCGCCCTGAGTTCACCGTCACCTCCTTCAAAATCGGGAAGCTCAACCTCACCGAAAACGCCGACGGCGCCAGCTCACACCTCGTCACTCGCTTCAACCTCACTCTGGCATCCAAAAACCCCAACAACCATCTTAGCTTCGCATATGAGTCCTTCGCGCTCACTCTCTCCGCCAACAACGACGTCGTTCTCGGCAACGGCTCGATCCCGGCCTTCTTCAGCGACACCAAGAACTTCACCTCCTTCCGGTCGATCATCACTGCTGCCGGCGACCTCGATGTGGAGTCGGCCAAGTCGCTGAGATCGGATCTGAGGCGGAAAGGCGGGGTTCCGATGAAGTTGCAGATGGACACCAAGGTGAAGGTGACGATTGGAGGGAAGCTGAAGAGCAAGAAGGTGGGGATTAGGGTTACGTGTCAGCAAATCAAGGGGGTAGCGCCGAAAGGTAAGTCGCCGTCGGTGGCGTCTGTTGCTGACTCCCACTGCAAGGTTGATCTTCGGATCAAGATCTGGAGATGGACCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

267

Amino Acids

29.17

Weight (kDa)

10.21

Isoelectric Point (pI)

31.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 126 - 230 5e-08 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 459
AccB1I GGYRCC 3 cut(s) 44, 153, 341
AccBSI CCGCTC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 15
AccII CGCG 1 cut(s) 431
AclWI GGATC 3 cut(s) 472, 588, 782
AcoI YGGCCR 2 cut(s) 90, 561
AcuI CTGAAG 2 cut(s) 475, 677
AcyI GRCGYC 4 cut(s) 45, 342, 456, 737
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 4 cut(s) 379, 519, 553, 760
AflIII ACRYGT 1 cut(s) 686
AgsI TTSAA 3 cut(s) 27, 304, 370
AleI CACNNNNGTG 1 cut(s) 629
AloI GAACNNNNNNTCC 2 cut(s) 497, 529
AluBI AGCT 4 cut(s) 316, 348, 411, 655
AluI AGCT 4 cut(s) 316, 348, 411, 655
Alw26I GTCTC 1 cut(s) 179
AlwI GGATC 3 cut(s) 472, 588, 782
AoxI GGCC 3 cut(s) 90, 483, 561
ApeKI GCWGC 5 cut(s) 103, 115, 180, 183, 536
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
AspLEI GCGC 5 cut(s) 47, 59, 344, 433, 712
AspS9I GGNCC 2 cut(s) 198, 792
AsuC2I CCSGG 1 cut(s) 482
AsuHPI GGTGA 6 cut(s) 280, 286, 316, 502, 643, 649
AvaII GGWCC 2 cut(s) 198, 792
BanI GGYRCC 3 cut(s) 44, 153, 341
BbsI GAAGAC 1 cut(s) 15
BbvCI CCTCAGC 1 cut(s) 129
BbvI GCAGC 5 cut(s) 115, 127, 167, 170, 523
BccI CCATC 3 cut(s) 411, 613, 783
BceAI ACGGC 7 cut(s) 58, 70, 105, 264, 355, 487, 712
BcgI CGANNNNNNTGC 2 cut(s) 595, 629
BcnI CCSGG 1 cut(s) 482
BcoDI GTCTC 1 cut(s) 179
BfmI CTRYAG 1 cut(s) 237
BfoI RGCGCY 4 cut(s) 48, 60, 345, 713
BglI GCCNNNNNGGC 2 cut(s) 112, 248
BglII AGATCT 1 cut(s) 780
Bme1390I CCNGG 1 cut(s) 482
Bme18I GGWCC 2 cut(s) 198, 792
BmgT120I GGNCC 2 cut(s) 198, 792
BmiI GGNNCC 4 cut(s) 46, 155, 343, 603
BmrFI CCNGG 1 cut(s) 482
BmsI GCATC 1 cut(s) 392
BpiI GAAGAC 1 cut(s) 15
Bpu10I CCTNAGC 1 cut(s) 129
BpuMI CCSGG 1 cut(s) 482
BsaAI YACGTR 1 cut(s) 687
BsaBI GATNNNNATC 1 cut(s) 779
BsaHI GRCGYC 4 cut(s) 45, 342, 456, 737
BsaJI CCNNGG 2 cut(s) 87, 627
BsaWI WCCGGW 2 cut(s) 168, 519
BsaXI ACNNNNNCTCC 2 cut(s) 497, 527
Bsc4I CCNNNNNNNGG 4 cut(s) 379, 519, 553, 760
Bse118I RCCGGY 2 cut(s) 242, 539
Bse8I GATNNNNATC 1 cut(s) 779
BseDI CCNNGG 2 cut(s) 87, 627
BseGI GGATG 1 cut(s) 383
BseJI GATNNNNATC 1 cut(s) 779
BseLI CCNNNNNNNGG 4 cut(s) 379, 519, 553, 760
BseMII CTCAG 4 cut(s) 143, 273, 563, 575
BseRI GAGGAG 1 cut(s) 218
BseXI GCAGC 5 cut(s) 115, 127, 167, 170, 523
Bsh1236I CGCG 1 cut(s) 431
Bsh1285I CGRYCG 1 cut(s) 524
BshFI GGCC 3 cut(s) 92, 485, 563
BshNI GGYRCC 3 cut(s) 44, 153, 341
BsiEI CGRYCG 1 cut(s) 524
BsiSI CCGG 6 cut(s) 112, 169, 243, 482, 520, 540
BslFI GGGAC 1 cut(s) 255
BslI CCNNNNNNNGG 4 cut(s) 379, 519, 553, 760
BsmAI GTCTC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 255
BsnI GGCC 3 cut(s) 92, 485, 563
Bsp143I GATC 7 cut(s) 477, 525, 575, 580, 766, 774, 780
BspANI GGCC 3 cut(s) 92, 485, 563
BspCNI CTCAG 4 cut(s) 142, 274, 564, 576
BspFNI CGCG 1 cut(s) 431
BspLI GGNNCC 4 cut(s) 46, 155, 343, 603
BspPI GGATC 3 cut(s) 472, 588, 782
BspQI GCTCTTC 1 cut(s) 653
BspT107I GGYRCC 3 cut(s) 44, 153, 341
BsrBI CCGCTC 1 cut(s) 109
BsrFI RCCGGY 2 cut(s) 242, 539
BssAI RCCGGY 2 cut(s) 242, 539
BssECI CCNNGG 2 cut(s) 87, 627
BssMI GATC 7 cut(s) 477, 525, 575, 580, 766, 774, 780
BssNI GRCGYC 4 cut(s) 45, 342, 456, 737
BssT1I CCWWGG 1 cut(s) 627
Bst4CI ACNGT 4 cut(s) 125, 149, 269, 292
Bst6I CTCTTC 1 cut(s) 653
BstACI GRCGYC 4 cut(s) 45, 342, 456, 737
BstBAI YACGTR 1 cut(s) 687
BstC8I GCNNGC 3 cut(s) 244, 346, 541
BstDEI CTNAG 5 cut(s) 129, 282, 407, 572, 584
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 1 cut(s) 383
BstFNI CGCG 1 cut(s) 431
BstH2I RGCGCY 4 cut(s) 48, 60, 345, 713
BstHHI GCGC 5 cut(s) 47, 59, 344, 433, 712
BstKTI GATC 7 cut(s) 480, 528, 578, 583, 769, 777, 783
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 7 cut(s) 477, 525, 575, 580, 766, 774, 780
BstMCI CGRYCG 1 cut(s) 524
BstMWI GCNNNNNNNGC 9 cut(s) 44, 106, 112, 115, 230, 239, 248, 341, 743
BstSCI CCNGG 1 cut(s) 480
BstSFI CTRYAG 1 cut(s) 237
BstUI CGCG 1 cut(s) 431
BstV1I GCAGC 5 cut(s) 115, 127, 167, 170, 523
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 2 cut(s) 580, 780
BstYI RGATCY 2 cut(s) 580, 780
BsuRI GGCC 3 cut(s) 92, 485, 563
BtgI CCRYGG 1 cut(s) 87
BtsCI GGATG 1 cut(s) 383
BtsI GCAGTG 2 cut(s) 531, 754
BtsIMutI CAGTG 2 cut(s) 531, 754
Cac8I GCNNGC 3 cut(s) 244, 346, 541
CfoI GCGC 5 cut(s) 47, 59, 344, 433, 712
Cfr10I RCCGGY 2 cut(s) 242, 539
Cfr13I GGNCC 2 cut(s) 198, 792
CseI GACGC 1 cut(s) 726
Csp6I GTAC 1 cut(s) 139
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 5 cut(s) 129, 282, 407, 572, 584
DinI GGCGCC 2 cut(s) 46, 343
DpnI GATC 7 cut(s) 479, 527, 577, 582, 768, 776, 782
DpnII GATC 7 cut(s) 477, 525, 575, 580, 766, 774, 780
EaeI YGGCCR 2 cut(s) 90, 561
Eam1104I CTCTTC 1 cut(s) 653
EarI CTCTTC 1 cut(s) 653
EciI GGCGGA 2 cut(s) 433, 604
Eco130I CCWWGG 1 cut(s) 627
Eco47I GGWCC 2 cut(s) 198, 792
Eco57I CTGAAG 2 cut(s) 475, 677
EcoT14I CCWWGG 1 cut(s) 627
EgeI GGCGCC 2 cut(s) 46, 343
EheI GGCGCC 2 cut(s) 46, 343
ErhI CCWWGG 1 cut(s) 627
FaiI YATR 5 cut(s) 203, 209, 239, 418, 420
FaqI GGGAC 1 cut(s) 255
FauI CCCGC 1 cut(s) 590
FauNDI CATATG 1 cut(s) 418
FblI GTMKAC 1 cut(s) 15
FokI GGATG 1 cut(s) 370
GlaI GCGC 5 cut(s) 46, 58, 343, 432, 711
HaeII RGCGCY 4 cut(s) 48, 60, 345, 713
HaeIII GGCC 3 cut(s) 92, 485, 563
HapII CCGG 6 cut(s) 112, 169, 243, 482, 520, 540
HgaI GACGC 1 cut(s) 726
HhaI GCGC 5 cut(s) 47, 59, 344, 433, 712
Hin1I GRCGYC 4 cut(s) 45, 342, 456, 737
Hin6I GCGC 5 cut(s) 45, 57, 342, 431, 710
HinP1I GCGC 5 cut(s) 45, 57, 342, 431, 710
HinfI GANTC 5 cut(s) 12, 172, 422, 557, 749
HpaII CCGG 6 cut(s) 112, 169, 243, 482, 520, 540
HphI GGTGA 6 cut(s) 280, 286, 316, 502, 643, 649
Hpy166II GTNNAC 2 cut(s) 16, 288
Hpy188I TCNGA 4 cut(s) 580, 585, 606, 774
Hpy188III TCNNGA 4 cut(s) 214, 310, 778, 784
Hpy8I GTNNAC 2 cut(s) 16, 288
Hpy99I CGWCG 5 cut(s) 153, 341, 458, 461, 733
HpyAV CCTTC 6 cut(s) 310, 436, 496, 526, 628, 661
HpyCH4III ACNGT 4 cut(s) 125, 149, 269, 292
HpyCH4IV ACGT 3 cut(s) 258, 456, 686
HpyCH4V TGCA 2 cut(s) 616, 759
HpyF10VI GCNNNNNNNGC 9 cut(s) 44, 106, 112, 115, 230, 239, 248, 341, 743
HpyF3I CTNAG 5 cut(s) 129, 282, 407, 572, 584
HpySE526I ACGT 3 cut(s) 258, 456, 686
Hsp92I GRCGYC 4 cut(s) 45, 342, 456, 737
HspAI GCGC 5 cut(s) 45, 57, 342, 431, 710
KasI GGCGCC 2 cut(s) 44, 341
KroI GCCGGC 2 cut(s) 242, 539
KroNI GCCGGC 2 cut(s) 244, 541
Kzo9I GATC 7 cut(s) 477, 525, 575, 580, 766, 774, 780
LguI GCTCTTC 1 cut(s) 653
LmnI GCTCC 1 cut(s) 114
Lsp1109I GCAGC 5 cut(s) 115, 127, 167, 170, 523
LweI GCATC 1 cut(s) 392
MaeII ACGT 3 cut(s) 258, 456, 686
MaeIII GTNAC 4 cut(s) 292, 358, 637, 682
MalI GATC 7 cut(s) 479, 527, 577, 582, 768, 776, 782
MbiI CCGCTC 1 cut(s) 109
MboI GATC 7 cut(s) 477, 525, 575, 580, 766, 774, 780
MboII GAAGA 4 cut(s) 15, 481, 670, 761
MflI RGATCY 2 cut(s) 580, 780
Mly113I GGCGCC 2 cut(s) 45, 342
MlyI GAGTC 5 cut(s) 21, 181, 431, 566, 743
MroNI GCCGGC 2 cut(s) 242, 539
MseI TTAA 1 cut(s) 799
MslI CAYNNNNRTG 1 cut(s) 629
MspI CCGG 6 cut(s) 112, 169, 243, 482, 520, 540
MspR9I CCNGG 1 cut(s) 482
MvnI CGCG 1 cut(s) 431
MwoI GCNNNNNNNGC 9 cut(s) 44, 106, 112, 115, 230, 239, 248, 341, 743
NaeI GCCGGC 2 cut(s) 244, 541
NarI GGCGCC 2 cut(s) 45, 342
NciI CCSGG 1 cut(s) 482
NdeI CATATG 1 cut(s) 418
NdeII GATC 7 cut(s) 477, 525, 575, 580, 766, 774, 780
NgoMIV GCCGGC 2 cut(s) 242, 539
NlaIV GGNNCC 4 cut(s) 46, 155, 343, 603
NmeAIII GCCGAG 1 cut(s) 444
NmuCI GTSAC 3 cut(s) 292, 358, 637
OliI CACNNNNGTG 1 cut(s) 629
PciSI GCTCTTC 1 cut(s) 653
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PdiI GCCGGC 2 cut(s) 244, 541
PflFI GACNNNGTC 1 cut(s) 11
PleI GAGTC 5 cut(s) 20, 180, 430, 565, 743
PluTI GGCGCC 2 cut(s) 48, 345
PpsI GAGTC 5 cut(s) 20, 180, 430, 565, 743
Ppu21I YACGTR 1 cut(s) 687
PspN4I GGNNCC 4 cut(s) 46, 155, 343, 603
PspPI GGNCC 2 cut(s) 198, 792
PsuI RGATCY 2 cut(s) 580, 780
PsyI GACNNNGTC 1 cut(s) 11
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 629
SapI GCTCTTC 1 cut(s) 653
SaqAI TTAA 1 cut(s) 799
Sau3AI GATC 7 cut(s) 477, 525, 575, 580, 766, 774, 780
Sau96I GGNCC 2 cut(s) 198, 792
SchI GAGTC 5 cut(s) 21, 181, 431, 566, 743
ScrFI CCNGG 1 cut(s) 482
SfaNI GCATC 1 cut(s) 392
SfcI CTRYAG 1 cut(s) 237
SfoI GGCGCC 2 cut(s) 46, 343
SinI GGWCC 2 cut(s) 198, 792
SmiMI CAYNNNNRTG 1 cut(s) 629
SspDI GGCGCC 2 cut(s) 44, 341
StyD4I CCNGG 1 cut(s) 480
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 4 cut(s) 125, 149, 269, 292
TaiI ACGT 3 cut(s) 261, 459, 689
TaqI TCGA 3 cut(s) 476, 524, 549
TauI GCSGC 6 cut(s) 38, 80, 103, 109, 136, 236
Tru1I TTAA 1 cut(s) 799
Tru9I TTAA 1 cut(s) 799
TscAI CASTG 2 cut(s) 538, 761
TseFI GTSAC 3 cut(s) 292, 358, 637
TseI GCWGC 5 cut(s) 103, 115, 180, 183, 536
Tsp45I GTSAC 3 cut(s) 292, 358, 637
TspDTI ATGAA 1 cut(s) 623
TspGWI ACGGA 1 cut(s) 20
TspRI CASTG 2 cut(s) 538, 761
Tth111I GACNNNGTC 1 cut(s) 11
VpaK11BI GGWCC 2 cut(s) 198, 792
XmiI GTMKAC 1 cut(s) 15
ZraI GACGTC 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.