MD15G1058900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011853
Physical Location & Seq
Reverse (-)
3934223 .. 3935221
999 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1058900.v1.1.491

Sequence Viewer

Length: 999 bp
ATGAAAGATCCATCCCTCTTGACATTGTGTGAGGTGGAAGCATCCCCCACCCCCCCCCCCGGCCCCACCTTTTTTTGGGTCCTCCATTTTCTTTCCGTTCTCATCCTTCTCACTCCACCATCTTCTTCTCTCTCTAAAACCTCACAGTGTCCACAACAATCCAGGACCACCTCTCTCAAATCCAAATCCAACCCATTTCTCACCATGACAGACCGAGTCTACCCATCTTCCAAACCCACCACCAACGGCGGCGCCGCCGCCGCAACAACCACGGGTACCGCAGCCAACCCACCAAGCACCAAACCCCAACTCAGACAACCCTACCGCCCGCAACCCCAGTACCAACACCACCGCCACCGCCGGAGCAACCGCAATTTATGCTGTTGCTGCTGCTTCTGGTCATTCCTTATCCTCATAGCCCTCGCACTCCTCGCCGCAATAGCCGGCGCCGCGGTCTACATCCTCTACCGCCCCCACCGCCCCGAGTTCACCCTCACCTCCGTCCGCATTGCGAAGCTCAACCTCACCACCACCCCCGACTTTTCCACCTCGCACCTCGCCACGCTCTTCAACCTCACCCTCTCCTCCAAAAACCCTAACAACCACCTCACCTTCTCCTACCAGCCGTTCAATCTGTTCCTCTCCACTACCAGAGACGTCCAAATCGCAAACGGGTCGATCCCGGCGTTCACCAGCGGCACGAAGAACAGCACCTTCTTCCGCTCCGTCCTGTCGACGTCGCAGGATCTCGACGTCGAGTCGGTGAAGTCGCTGAGATCGGATCTGAAGAAGCAGAGAGGGGTGGCGCTGAAGCTGGAGATGGACACCAAAGTGAAGGTGTCGATGGGGAAGCTGAGTAGCAAGAAGGTGGGGATTAGGGTTACGTGTCAAGGAATCAAAGGGGTTGTACCAAAAGGTAAGTCACCGTCGGTGGCTGCCGTTTCCAACTCCAAGTGCAAGGTTGATCTTCGGATCAGGATCTGGAAATGGACCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

333

Amino Acids

36.55

Weight (kDa)

10.19

Isoelectric Point (pI)

34.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 193 - 296 1.9e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 660, 740, 756
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 3 cut(s) 251, 275, 446
AccBSI CCGCTC 1 cut(s) 723
AccI GTMKAC 3 cut(s) 219, 456, 734
AccII CGCG 1 cut(s) 452
AclWI GGATC 6 cut(s) 2, 673, 753, 789, 980, 986
AcuI CTGAAG 2 cut(s) 806, 830
AcyI GRCGYC 5 cut(s) 252, 447, 657, 737, 753
AfaI GTAC 3 cut(s) 277, 341, 909
AfiI CCNNNNNNNGG 2 cut(s) 59, 75
AflIII ACRYGT 1 cut(s) 884
AgsI TTSAA 2 cut(s) 571, 631
AhdI GACNNNNNGTC 1 cut(s) 757
AjnI CCWGG 1 cut(s) 161
AleI CACNNNNGTG 1 cut(s) 830
AluBI AGCT 3 cut(s) 517, 814, 853
AluI AGCT 3 cut(s) 517, 814, 853
Alw26I GTCTC 1 cut(s) 648
AlwI GGATC 6 cut(s) 2, 673, 753, 789, 980, 986
AlwNI CAGNNNCTG 1 cut(s) 981
Ama87I CYCGRG 1 cut(s) 482
AoxI GGCC 1 cut(s) 61
ApeKI GCWGC 4 cut(s) 281, 387, 390, 935
ArsI GACNNNNNNTTYG 2 cut(s) 905, 937
Asp718I GGTACC 1 cut(s) 275
AspLEI GCGC 3 cut(s) 254, 449, 808
AspS9I GGNCC 4 cut(s) 62, 79, 165, 990
AsuC2I CCSGG 2 cut(s) 60, 683
AsuHPI GGTGA 9 cut(s) 193, 481, 487, 517, 568, 601, 682, 775, 915
AvaI CYCGRG 1 cut(s) 482
AvaII GGWCC 3 cut(s) 79, 165, 990
BaeI ACNNNNGTAYC 2 cut(s) 323, 356
BanI GGYRCC 3 cut(s) 251, 275, 446
BbvI GCAGC 4 cut(s) 293, 374, 377, 922
BccI CCATC 5 cut(s) 19, 127, 232, 814, 838
BceAI ACGGC 3 cut(s) 262, 610, 923
BcgI CGANNNNNNTGC 2 cut(s) 657, 691
BciT130I CCWGG 1 cut(s) 163
BcnI CCSGG 2 cut(s) 60, 683
BcoDI GTCTC 1 cut(s) 648
BfoI RGCGCY 3 cut(s) 255, 450, 809
Bme1390I CCNGG 3 cut(s) 60, 163, 683
Bme18I GGWCC 3 cut(s) 79, 165, 990
BmeRI GACNNNNNGTC 1 cut(s) 757
BmeT110I CYCGRG 1 cut(s) 482
BmgT120I GGNCC 4 cut(s) 62, 79, 165, 990
BmiI GGNNCC 5 cut(s) 64, 80, 253, 277, 448
BmrFI CCNGG 3 cut(s) 60, 163, 683
BmrI ACTGGG 1 cut(s) 331
BmsI GCATC 1 cut(s) 50
BmuI ACTGGG 1 cut(s) 331
BpmI CTGGAG 1 cut(s) 836
BpuMI CCSGG 2 cut(s) 60, 683
BsaAI YACGTR 1 cut(s) 885
BsaBI GATNNNNATC 1 cut(s) 977
BsaHI GRCGYC 5 cut(s) 252, 447, 657, 737, 753
BsaJI CCNNGG 3 cut(s) 58, 270, 450
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 75
Bse118I RCCGGY 1 cut(s) 443
Bse1I ACTGG 1 cut(s) 337
Bse3DI GCAATG 1 cut(s) 507
Bse8I GATNNNNATC 1 cut(s) 977
BseBI CCWGG 1 cut(s) 163
BseDI CCNNGG 3 cut(s) 58, 270, 450
BseGI GGATG 4 cut(s) 11, 41, 102, 459
BseJI GATNNNNATC 1 cut(s) 977
BseLI CCNNNNNNNGG 2 cut(s) 59, 75
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 3 cut(s) 325, 764, 845
BseNI ACTGG 1 cut(s) 337
BseRI GAGGAG 2 cut(s) 419, 574
BseXI GCAGC 4 cut(s) 293, 374, 377, 922
Bsh1236I CGCG 1 cut(s) 452
BshFI GGCC 1 cut(s) 63
BshNI GGYRCC 3 cut(s) 251, 275, 446
BsiHKCI CYCGRG 1 cut(s) 482
BsiSI CCGG 4 cut(s) 60, 361, 444, 683
BslI CCNNNNNNNGG 2 cut(s) 59, 75
BsmAI GTCTC 1 cut(s) 648
BsmBI CGTCTC 1 cut(s) 648
BsnI GGCC 1 cut(s) 63
BsoBI CYCGRG 1 cut(s) 482
Bsp143I GATC 8 cut(s) 7, 678, 745, 776, 781, 964, 972, 978
BspANI GGCC 1 cut(s) 63
BspCNI CTCAG 3 cut(s) 324, 765, 846
BspFNI CGCG 1 cut(s) 452
BspLI GGNNCC 5 cut(s) 64, 80, 253, 277, 448
BspPI GGATC 6 cut(s) 2, 673, 753, 789, 980, 986
BspQI GCTCTTC 1 cut(s) 572
BspT107I GGYRCC 3 cut(s) 251, 275, 446
BsrBI CCGCTC 1 cut(s) 723
BsrDI GCAATG 1 cut(s) 507
BsrFI RCCGGY 1 cut(s) 443
BsrI ACTGG 1 cut(s) 337
BssAI RCCGGY 1 cut(s) 443
BssECI CCNNGG 3 cut(s) 58, 270, 450
BssMI GATC 8 cut(s) 7, 678, 745, 776, 781, 964, 972, 978
BssNI GRCGYC 5 cut(s) 252, 447, 657, 737, 753
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 2 cut(s) 147, 927
Bst6I CTCTTC 1 cut(s) 572
BstACI GRCGYC 5 cut(s) 252, 447, 657, 737, 753
BstAPI GCANNNNNTGC 1 cut(s) 378
BstBAI YACGTR 1 cut(s) 885
BstC8I GCNNGC 2 cut(s) 329, 445
BstDEI CTNAG 3 cut(s) 311, 773, 854
BstDSI CCRYGG 2 cut(s) 270, 450
BstF5I GGATG 4 cut(s) 11, 41, 102, 459
BstFNI CGCG 1 cut(s) 452
BstH2I RGCGCY 3 cut(s) 255, 450, 809
BstHHI GCGC 3 cut(s) 254, 449, 808
BstKTI GATC 8 cut(s) 10, 681, 748, 779, 784, 967, 975, 981
BstMAI GTCTC 1 cut(s) 648
BstMBI GATC 8 cut(s) 7, 678, 745, 776, 781, 964, 972, 978
BstMWI GCNNNNNNNGC 7 cut(s) 260, 378, 387, 431, 440, 449, 477
BstNI CCWGG 1 cut(s) 163
BstSCI CCNGG 3 cut(s) 58, 161, 681
BstUI CGCG 1 cut(s) 452
BstV1I GCAGC 4 cut(s) 293, 374, 377, 922
BstX2I RGATCY 4 cut(s) 7, 745, 781, 978
BstYI RGATCY 4 cut(s) 7, 745, 781, 978
BsuRI GGCC 1 cut(s) 63
BtgI CCRYGG 2 cut(s) 270, 450
BtsCI GGATG 4 cut(s) 11, 41, 102, 459
BtsIMutI CAGTG 1 cut(s) 152
Cac8I GCNNGC 2 cut(s) 329, 445
CaiI CAGNNNCTG 1 cut(s) 981
CfoI GCGC 3 cut(s) 254, 449, 808
Cfr10I RCCGGY 1 cut(s) 443
Cfr13I GGNCC 4 cut(s) 62, 79, 165, 990
Cfr42I CCGCGG 1 cut(s) 453
Csp6I GTAC 3 cut(s) 276, 340, 908
CviAII CATG 1 cut(s) 205
CviJI RGCY 9 cut(s) 63, 284, 419, 443, 517, 625, 814, 853, 935
CviKI_1 RGCY 9 cut(s) 63, 284, 419, 443, 517, 625, 814, 853, 935
CviQI GTAC 3 cut(s) 276, 340, 908
DdeI CTNAG 3 cut(s) 311, 773, 854
DinI GGCGCC 2 cut(s) 253, 448
DpnI GATC 8 cut(s) 9, 680, 747, 778, 783, 966, 974, 980
DpnII GATC 8 cut(s) 7, 678, 745, 776, 781, 964, 972, 978
DriI GACNNNNNGTC 1 cut(s) 757
Eam1104I CTCTTC 1 cut(s) 572
Eam1105I GACNNNNNGTC 1 cut(s) 757
EarI CTCTTC 1 cut(s) 572
Eco47I GGWCC 3 cut(s) 79, 165, 990
Eco57I CTGAAG 2 cut(s) 806, 830
Eco88I CYCGRG 1 cut(s) 482
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 161
EgeI GGCGCC 2 cut(s) 253, 448
EheI GGCGCC 2 cut(s) 253, 448
Esp3I CGTCTC 1 cut(s) 648
FaeI CATG 1 cut(s) 208
FaiI YATR 3 cut(s) 206, 379, 416
FatI CATG 1 cut(s) 204
FauI CCCGC 1 cut(s) 336
FblI GTMKAC 3 cut(s) 219, 456, 734
FokI GGATG 3 cut(s) 28, 89, 446
GlaI GCGC 3 cut(s) 253, 448, 807
GsuI CTGGAG 1 cut(s) 836
HaeII RGCGCY 3 cut(s) 255, 450, 809
HaeIII GGCC 1 cut(s) 63
HapII CCGG 4 cut(s) 60, 361, 444, 683
HhaI GCGC 3 cut(s) 254, 449, 808
Hin1I GRCGYC 5 cut(s) 252, 447, 657, 737, 753
Hin1II CATG 1 cut(s) 208
Hin6I GCGC 3 cut(s) 252, 447, 806
HinP1I GCGC 3 cut(s) 252, 447, 806
HincII GTYRAC 1 cut(s) 735
HindII GTYRAC 1 cut(s) 735
HinfI GANTC 3 cut(s) 216, 758, 894
HpaII CCGG 4 cut(s) 60, 361, 444, 683
HphI GGTGA 9 cut(s) 193, 481, 487, 517, 568, 601, 682, 775, 915
Hpy166II GTNNAC 6 cut(s) 152, 220, 457, 489, 690, 735
Hpy188I TCNGA 4 cut(s) 314, 781, 786, 972
Hpy188III TCNNGA 4 cut(s) 19, 749, 976, 982
Hpy8I GTNNAC 6 cut(s) 152, 220, 457, 489, 690, 735
Hpy99I CGWCG 5 cut(s) 739, 742, 755, 758, 931
HpyAV CCTTC 5 cut(s) 116, 622, 724, 829, 859
HpyCH4III ACNGT 2 cut(s) 147, 927
HpyCH4IV ACGT 4 cut(s) 657, 737, 753, 884
HpyCH4V TGCA 1 cut(s) 957
HpyF10VI GCNNNNNNNGC 7 cut(s) 260, 378, 387, 431, 440, 449, 477
HpyF3I CTNAG 3 cut(s) 311, 773, 854
HpySE526I ACGT 4 cut(s) 657, 737, 753, 884
Hsp92I GRCGYC 5 cut(s) 252, 447, 657, 737, 753
Hsp92II CATG 1 cut(s) 208
HspAI GCGC 3 cut(s) 252, 447, 806
KasI GGCGCC 2 cut(s) 251, 446
KpnI GGTACC 1 cut(s) 279
KroI GCCGGC 1 cut(s) 443
KroNI GCCGGC 1 cut(s) 445
KspI CCGCGG 1 cut(s) 453
Kzo9I GATC 8 cut(s) 7, 678, 745, 776, 781, 964, 972, 978
LguI GCTCTTC 1 cut(s) 572
LmnI GCTCC 2 cut(s) 363, 728
Lsp1109I GCAGC 4 cut(s) 293, 374, 377, 922
LweI GCATC 1 cut(s) 50
MaeII ACGT 4 cut(s) 657, 737, 753, 884
MaeIII GTNAC 2 cut(s) 880, 921
MalI GATC 8 cut(s) 9, 680, 747, 778, 783, 966, 974, 980
MbiI CCGCTC 1 cut(s) 723
MboI GATC 8 cut(s) 7, 678, 745, 776, 781, 964, 972, 978
MboII GAAGA 8 cut(s) 114, 117, 219, 559, 709, 715, 799, 959
MflI RGATCY 4 cut(s) 7, 745, 781, 978
MluCI AATT 1 cut(s) 373
Mly113I GGCGCC 2 cut(s) 252, 447
MlyI GAGTC 2 cut(s) 225, 767
MmeI TCCRAC 2 cut(s) 213, 969
MroNI GCCGGC 1 cut(s) 443
MseI TTAA 1 cut(s) 997
MslI CAYNNNNRTG 1 cut(s) 830
MspA1I CMGCKG 2 cut(s) 452, 696
MspI CCGG 4 cut(s) 60, 361, 444, 683
MspR9I CCNGG 3 cut(s) 60, 163, 683
MvaI CCWGG 1 cut(s) 163
MvnI CGCG 1 cut(s) 452
MwoI GCNNNNNNNGC 7 cut(s) 260, 378, 387, 431, 440, 449, 477
NaeI GCCGGC 1 cut(s) 445
NarI GGCGCC 2 cut(s) 252, 447
NciI CCSGG 2 cut(s) 60, 683
NdeII GATC 8 cut(s) 7, 678, 745, 776, 781, 964, 972, 978
NgoMIV GCCGGC 1 cut(s) 443
NlaIII CATG 1 cut(s) 208
NlaIV GGNNCC 5 cut(s) 64, 80, 253, 277, 448
NmuCI GTSAC 1 cut(s) 921
OliI CACNNNNGTG 1 cut(s) 830
PciSI GCTCTTC 1 cut(s) 572
PcsI WCGNNNNNNNCGW 1 cut(s) 683
PdiI GCCGGC 1 cut(s) 445
PfeI GAWTC 1 cut(s) 894
PflFI GACNNNGTC 1 cut(s) 215
PfoI TCCNGGA 1 cut(s) 161
PleI GAGTC 2 cut(s) 224, 766
PluTI GGCGCC 2 cut(s) 255, 450
PpsI GAGTC 2 cut(s) 224, 766
Ppu21I YACGTR 1 cut(s) 885
PpuMI RGGWCCY 1 cut(s) 79
Psp5II RGGWCCY 1 cut(s) 79
Psp6I CCWGG 1 cut(s) 161
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 5 cut(s) 64, 80, 253, 277, 448
PspPI GGNCC 4 cut(s) 62, 79, 165, 990
PspPPI RGGWCCY 1 cut(s) 79
PstNI CAGNNNCTG 1 cut(s) 981
PsuI RGATCY 4 cut(s) 7, 745, 781, 978
PsyI GACNNNGTC 1 cut(s) 215
RsaI GTAC 3 cut(s) 277, 341, 909
RsaNI GTAC 3 cut(s) 276, 340, 908
RseI CAYNNNNRTG 1 cut(s) 830
SacII CCGCGG 1 cut(s) 453
SalI GTCGAC 1 cut(s) 733
SapI GCTCTTC 1 cut(s) 572
SaqAI TTAA 1 cut(s) 997
Sau3AI GATC 8 cut(s) 7, 678, 745, 776, 781, 964, 972, 978
Sau96I GGNCC 4 cut(s) 62, 79, 165, 990
SchI GAGTC 2 cut(s) 225, 767
ScrFI CCNGG 3 cut(s) 60, 163, 683
SfaNI GCATC 1 cut(s) 50
SfoI GGCGCC 2 cut(s) 253, 448
Sfr303I CCGCGG 1 cut(s) 453
SgrBI CCGCGG 1 cut(s) 453
SinI GGWCC 3 cut(s) 79, 165, 990
SmiMI CAYNNNNRTG 1 cut(s) 830
Sse9I AATT 1 cut(s) 373
SspDI GGCGCC 2 cut(s) 251, 446
StyD4I CCNGG 3 cut(s) 58, 161, 681
TaaI ACNGT 2 cut(s) 147, 927
TaiI ACGT 4 cut(s) 660, 740, 756, 887
TaqI TCGA 5 cut(s) 677, 734, 750, 756, 842
TaqII GACCGA 1 cut(s) 228
TasI AATT 1 cut(s) 373
TauI GCSGC 7 cut(s) 252, 257, 260, 263, 437, 452, 699
TfiI GAWTC 1 cut(s) 894
Tru1I TTAA 1 cut(s) 997
Tru9I TTAA 1 cut(s) 997
TscAI CASTG 1 cut(s) 152
TseFI GTSAC 1 cut(s) 921
TseI GCWGC 4 cut(s) 281, 387, 390, 935
Tsp45I GTSAC 1 cut(s) 921
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 85, 490, 715
TspRI CASTG 1 cut(s) 152
Tth111I GACNNNGTC 1 cut(s) 215
VpaK11BI GGWCC 3 cut(s) 79, 165, 990
XmiI GTMKAC 3 cut(s) 219, 456, 734
ZraI GACGTC 3 cut(s) 658, 738, 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.