pycom15g05530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011853
Physical Location & Seq
Reverse (-)
3295565 .. 3296386
822 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g05530.1

Sequence Viewer

Length: 822 bp
ATGACAGACCGAGTCTACCCATCTTCCAAACCCACCACCAACGGCGCCGCCGCCGTACCCGGCGCCGCAACAACCACGGGTACCGCAGCCACCACGGGTACCGCAGCCAACCCACCAAGCACCAAACCCCAACTCAGACAACCCTACCGCCCGAAACCCCAGTACCAACACCGCCGCCACCGCCGGAGCAACCGCAATTTATGCTGTTGCTGCTGCTTCTGGTCATTCCTTATCCTCATAGCCCTCGCACTCCTCGCCGCAATAGCCGGCGCCGCGGTCTACATCCTCTACCGCCCCCACCGCCCCGAGTTCACCCTCACCTCCGTCCGCATTGCGAAGCTCAACCTCACCACCACCCCCGACTTTTCCACCTCGCACCTCGCCACCCTCTTCAACCTCACCCTCTCCTCCAAAAACCCCAACAACCACCTCACCTTCTCCTACCAGCCGTTCAATCTGTTCCTCTCCACCACCAGAGACGTCCAAATCGCAAATGGGTCGATCCCGGCGTTCACCAGCGGCACAAAGAACAGCACCTTCTTCCGCCCCGTCCTGTCGACGTCGCAGGATCTCGACGTCGAGTCGGTGAAGTCGCTGAGATCGGATCTGAAGAAGCAGAGCGGGGTGGCGCTGAAGCTACAGATGGACACCAAAGTGAAGGTGTCGATGGGGAAGCTGAGTACCAAGAAGGTGGGGATTAGGGTTACGTGTCAAGGAATCAAAGGGGTTGTACCAAAAGGTAAGTCACCGTCGGTGGCTGCCGTTTCCAACTCCAAGTGCAAGGTTGATCTTCGGATCAGGATCTGGAAATGGACCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

274

Amino Acids

29.89

Weight (kDa)

10.44

Isoelectric Point (pI)

29.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 134 - 237 1.3e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 483, 563, 579
Acc65I GGTACC 2 cut(s) 80, 98
AccB1I GGYRCC 5 cut(s) 44, 62, 80, 98, 269
AccBSI CCGCTC 1 cut(s) 621
AccI GTMKAC 3 cut(s) 15, 279, 557
AccII CGCG 1 cut(s) 275
AclWI GGATC 5 cut(s) 496, 576, 612, 803, 809
AcuI CTGAAG 2 cut(s) 629, 653
AcyI GRCGYC 6 cut(s) 45, 63, 270, 480, 560, 576
AfaI GTAC 6 cut(s) 57, 82, 100, 164, 682, 732
AflIII ACRYGT 1 cut(s) 707
AgsI TTSAA 2 cut(s) 394, 454
AhdI GACNNNNNGTC 1 cut(s) 580
AleI CACNNNNGTG 1 cut(s) 653
AluBI AGCT 3 cut(s) 340, 637, 676
AluI AGCT 3 cut(s) 340, 637, 676
Alw26I GTCTC 1 cut(s) 471
AlwI GGATC 5 cut(s) 496, 576, 612, 803, 809
AlwNI CAGNNNCTG 1 cut(s) 804
Ama87I CYCGRG 1 cut(s) 305
ApeKI GCWGC 5 cut(s) 86, 104, 210, 213, 758
ArsI GACNNNNNNTTYG 2 cut(s) 728, 760
Asp718I GGTACC 2 cut(s) 80, 98
AspLEI GCGC 4 cut(s) 47, 65, 272, 631
AspS9I GGNCC 1 cut(s) 813
AsuC2I CCSGG 2 cut(s) 60, 506
AsuHPI GGTGA 8 cut(s) 304, 310, 340, 391, 424, 505, 598, 738
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 1 cut(s) 813
BaeI ACNNNNGTAYC 2 cut(s) 146, 179
BanI GGYRCC 5 cut(s) 44, 62, 80, 98, 269
BbvI GCAGC 5 cut(s) 98, 116, 197, 200, 745
BccI CCATC 3 cut(s) 28, 637, 661
BceAI ACGGC 4 cut(s) 38, 58, 433, 746
BcgI CGANNNNNNTGC 2 cut(s) 480, 514
BcnI CCSGG 2 cut(s) 60, 506
BcoDI GTCTC 1 cut(s) 471
BfmI CTRYAG 1 cut(s) 638
BfoI RGCGCY 4 cut(s) 48, 66, 273, 632
Bme1390I CCNGG 2 cut(s) 60, 506
Bme18I GGWCC 1 cut(s) 813
BmeRI GACNNNNNGTC 1 cut(s) 580
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 813
BmiI GGNNCC 5 cut(s) 46, 64, 82, 100, 271
BmrFI CCNGG 2 cut(s) 60, 506
BmrI ACTGGG 1 cut(s) 154
BmuI ACTGGG 1 cut(s) 154
BpuMI CCSGG 2 cut(s) 60, 506
BsaAI YACGTR 1 cut(s) 708
BsaBI GATNNNNATC 1 cut(s) 800
BsaHI GRCGYC 6 cut(s) 45, 63, 270, 480, 560, 576
BsaJI CCNNGG 3 cut(s) 75, 93, 273
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 160
Bse3DI GCAATG 1 cut(s) 330
Bse8I GATNNNNATC 1 cut(s) 800
BseDI CCNNGG 3 cut(s) 75, 93, 273
BseGI GGATG 1 cut(s) 282
BseJI GATNNNNATC 1 cut(s) 800
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 3 cut(s) 148, 587, 668
BseNI ACTGG 1 cut(s) 160
BseRI GAGGAG 2 cut(s) 242, 397
BseXI GCAGC 5 cut(s) 98, 116, 197, 200, 745
Bsh1236I CGCG 1 cut(s) 275
BshNI GGYRCC 5 cut(s) 44, 62, 80, 98, 269
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 4 cut(s) 60, 184, 267, 506
BsmAI GTCTC 1 cut(s) 471
BsmBI CGTCTC 1 cut(s) 471
BsoBI CYCGRG 1 cut(s) 305
Bsp143I GATC 7 cut(s) 501, 568, 599, 604, 787, 795, 801
BspCNI CTCAG 3 cut(s) 147, 588, 669
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 5 cut(s) 46, 64, 82, 100, 271
BspPI GGATC 5 cut(s) 496, 576, 612, 803, 809
BspT107I GGYRCC 5 cut(s) 44, 62, 80, 98, 269
BsrBI CCGCTC 1 cut(s) 621
BsrDI GCAATG 1 cut(s) 330
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 1 cut(s) 160
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 3 cut(s) 75, 93, 273
BssMI GATC 7 cut(s) 501, 568, 599, 604, 787, 795, 801
BssNI GRCGYC 6 cut(s) 45, 63, 270, 480, 560, 576
Bst4CI ACNGT 1 cut(s) 750
Bst6I CTCTTC 1 cut(s) 395
BstACI GRCGYC 6 cut(s) 45, 63, 270, 480, 560, 576
BstAPI GCANNNNNTGC 1 cut(s) 201
BstBAI YACGTR 1 cut(s) 708
BstC8I GCNNGC 1 cut(s) 268
BstDEI CTNAG 3 cut(s) 134, 596, 677
BstDSI CCRYGG 3 cut(s) 75, 93, 273
BstF5I GGATG 1 cut(s) 282
BstFNI CGCG 1 cut(s) 275
BstH2I RGCGCY 4 cut(s) 48, 66, 273, 632
BstHHI GCGC 4 cut(s) 47, 65, 272, 631
BstKTI GATC 7 cut(s) 504, 571, 602, 607, 790, 798, 804
BstMAI GTCTC 1 cut(s) 471
BstMBI GATC 7 cut(s) 501, 568, 599, 604, 787, 795, 801
BstMWI GCNNNNNNNGC 7 cut(s) 180, 201, 210, 254, 263, 272, 300
BstSCI CCNGG 2 cut(s) 58, 504
BstSFI CTRYAG 1 cut(s) 638
BstUI CGCG 1 cut(s) 275
BstV1I GCAGC 5 cut(s) 98, 116, 197, 200, 745
BstX2I RGATCY 3 cut(s) 568, 604, 801
BstXI CCANNNNNNTGG 1 cut(s) 691
BstYI RGATCY 3 cut(s) 568, 604, 801
BtgI CCRYGG 3 cut(s) 75, 93, 273
BtsCI GGATG 1 cut(s) 282
Cac8I GCNNGC 1 cut(s) 268
CaiI CAGNNNCTG 1 cut(s) 804
CfoI GCGC 4 cut(s) 47, 65, 272, 631
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 1 cut(s) 813
Cfr42I CCGCGG 1 cut(s) 276
Csp6I GTAC 6 cut(s) 56, 81, 99, 163, 681, 731
CviJI RGCY 9 cut(s) 89, 107, 242, 266, 340, 448, 637, 676, 758
CviKI_1 RGCY 9 cut(s) 89, 107, 242, 266, 340, 448, 637, 676, 758
CviQI GTAC 6 cut(s) 56, 81, 99, 163, 681, 731
DdeI CTNAG 3 cut(s) 134, 596, 677
DinI GGCGCC 3 cut(s) 46, 64, 271
DpnI GATC 7 cut(s) 503, 570, 601, 606, 789, 797, 803
DpnII GATC 7 cut(s) 501, 568, 599, 604, 787, 795, 801
DriI GACNNNNNGTC 1 cut(s) 580
Eam1104I CTCTTC 1 cut(s) 395
Eam1105I GACNNNNNGTC 1 cut(s) 580
EarI CTCTTC 1 cut(s) 395
EciI GGCGGA 1 cut(s) 533
Eco47I GGWCC 1 cut(s) 813
Eco57I CTGAAG 2 cut(s) 629, 653
Eco88I CYCGRG 1 cut(s) 305
EgeI GGCGCC 3 cut(s) 46, 64, 271
EheI GGCGCC 3 cut(s) 46, 64, 271
Esp3I CGTCTC 1 cut(s) 471
FaiI YATR 2 cut(s) 202, 239
FauI CCCGC 1 cut(s) 614
FblI GTMKAC 3 cut(s) 15, 279, 557
FokI GGATG 1 cut(s) 269
GlaI GCGC 4 cut(s) 46, 64, 271, 630
HaeII RGCGCY 4 cut(s) 48, 66, 273, 632
HapII CCGG 4 cut(s) 60, 184, 267, 506
HhaI GCGC 4 cut(s) 47, 65, 272, 631
Hin1I GRCGYC 6 cut(s) 45, 63, 270, 480, 560, 576
Hin6I GCGC 4 cut(s) 45, 63, 270, 629
HinP1I GCGC 4 cut(s) 45, 63, 270, 629
HincII GTYRAC 1 cut(s) 558
HindII GTYRAC 1 cut(s) 558
HinfI GANTC 3 cut(s) 12, 581, 717
HpaII CCGG 4 cut(s) 60, 184, 267, 506
HphI GGTGA 8 cut(s) 304, 310, 340, 391, 424, 505, 598, 738
Hpy166II GTNNAC 5 cut(s) 16, 280, 312, 513, 558
Hpy188I TCNGA 4 cut(s) 137, 604, 609, 795
Hpy188III TCNNGA 3 cut(s) 572, 799, 805
Hpy8I GTNNAC 5 cut(s) 16, 280, 312, 513, 558
Hpy99I CGWCG 5 cut(s) 562, 565, 578, 581, 754
HpyAV CCTTC 4 cut(s) 445, 547, 652, 682
HpyCH4III ACNGT 1 cut(s) 750
HpyCH4IV ACGT 4 cut(s) 480, 560, 576, 707
HpyCH4V TGCA 1 cut(s) 780
HpyF10VI GCNNNNNNNGC 7 cut(s) 180, 201, 210, 254, 263, 272, 300
HpyF3I CTNAG 3 cut(s) 134, 596, 677
HpySE526I ACGT 4 cut(s) 480, 560, 576, 707
Hsp92I GRCGYC 6 cut(s) 45, 63, 270, 480, 560, 576
HspAI GCGC 4 cut(s) 45, 63, 270, 629
KasI GGCGCC 3 cut(s) 44, 62, 269
KpnI GGTACC 2 cut(s) 84, 102
KroI GCCGGC 1 cut(s) 266
KroNI GCCGGC 1 cut(s) 268
KspI CCGCGG 1 cut(s) 276
Kzo9I GATC 7 cut(s) 501, 568, 599, 604, 787, 795, 801
LmnI GCTCC 1 cut(s) 186
Lsp1109I GCAGC 5 cut(s) 98, 116, 197, 200, 745
MaeII ACGT 4 cut(s) 480, 560, 576, 707
MaeIII GTNAC 2 cut(s) 703, 744
MalI GATC 7 cut(s) 503, 570, 601, 606, 789, 797, 803
MbiI CCGCTC 1 cut(s) 621
MboI GATC 7 cut(s) 501, 568, 599, 604, 787, 795, 801
MboII GAAGA 5 cut(s) 15, 382, 532, 622, 782
MflI RGATCY 3 cut(s) 568, 604, 801
MluCI AATT 1 cut(s) 196
Mly113I GGCGCC 3 cut(s) 45, 63, 270
MlyI GAGTC 2 cut(s) 21, 590
MmeI TCCRAC 1 cut(s) 792
MroNI GCCGGC 1 cut(s) 266
MseI TTAA 1 cut(s) 820
MslI CAYNNNNRTG 1 cut(s) 653
MspA1I CMGCKG 2 cut(s) 275, 519
MspI CCGG 4 cut(s) 60, 184, 267, 506
MspR9I CCNGG 2 cut(s) 60, 506
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 7 cut(s) 180, 201, 210, 254, 263, 272, 300
NaeI GCCGGC 1 cut(s) 268
NarI GGCGCC 3 cut(s) 45, 63, 270
NciI CCSGG 2 cut(s) 60, 506
NdeII GATC 7 cut(s) 501, 568, 599, 604, 787, 795, 801
NgoMIV GCCGGC 1 cut(s) 266
NlaIV GGNNCC 5 cut(s) 46, 64, 82, 100, 271
NmuCI GTSAC 1 cut(s) 744
OliI CACNNNNGTG 1 cut(s) 653
PcsI WCGNNNNNNNCGW 1 cut(s) 506
PdiI GCCGGC 1 cut(s) 268
PfeI GAWTC 1 cut(s) 717
PflFI GACNNNGTC 1 cut(s) 11
PleI GAGTC 2 cut(s) 20, 589
PluTI GGCGCC 3 cut(s) 48, 66, 273
PpsI GAGTC 2 cut(s) 20, 589
Ppu21I YACGTR 1 cut(s) 708
PspN4I GGNNCC 5 cut(s) 46, 64, 82, 100, 271
PspPI GGNCC 1 cut(s) 813
PstNI CAGNNNCTG 1 cut(s) 804
PsuI RGATCY 3 cut(s) 568, 604, 801
PsyI GACNNNGTC 1 cut(s) 11
RsaI GTAC 6 cut(s) 57, 82, 100, 164, 682, 732
RsaNI GTAC 6 cut(s) 56, 81, 99, 163, 681, 731
RseI CAYNNNNRTG 1 cut(s) 653
SacII CCGCGG 1 cut(s) 276
SalI GTCGAC 1 cut(s) 556
SaqAI TTAA 1 cut(s) 820
Sau3AI GATC 7 cut(s) 501, 568, 599, 604, 787, 795, 801
Sau96I GGNCC 1 cut(s) 813
SchI GAGTC 2 cut(s) 21, 590
ScrFI CCNGG 2 cut(s) 60, 506
SfcI CTRYAG 1 cut(s) 638
SfoI GGCGCC 3 cut(s) 46, 64, 271
Sfr303I CCGCGG 1 cut(s) 276
SgrBI CCGCGG 1 cut(s) 276
SinI GGWCC 1 cut(s) 813
SmiMI CAYNNNNRTG 1 cut(s) 653
Sse9I AATT 1 cut(s) 196
SspDI GGCGCC 3 cut(s) 44, 62, 269
StyD4I CCNGG 2 cut(s) 58, 504
TaaI ACNGT 1 cut(s) 750
TaiI ACGT 4 cut(s) 483, 563, 579, 710
TaqI TCGA 5 cut(s) 500, 557, 573, 579, 665
TaqII GACCGA 1 cut(s) 24
TasI AATT 1 cut(s) 196
TauI GCSGC 7 cut(s) 50, 53, 68, 177, 260, 275, 522
TfiI GAWTC 1 cut(s) 717
Tru1I TTAA 1 cut(s) 820
Tru9I TTAA 1 cut(s) 820
TseFI GTSAC 1 cut(s) 744
TseI GCWGC 5 cut(s) 86, 104, 210, 213, 758
Tsp45I GTSAC 1 cut(s) 744
TspGWI ACGGA 1 cut(s) 313
Tth111I GACNNNGTC 1 cut(s) 11
VpaK11BI GGWCC 1 cut(s) 813
XcmI CCANNNNNNNNNTGG 1 cut(s) 491
XmiI GTMKAC 3 cut(s) 15, 279, 557
ZraI GACGTC 3 cut(s) 481, 561, 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.