Rmu_sc0028522.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0028522.1
OG0011853
Physical Location & Seq
Reverse (-)
2 .. 574
573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0028522.1_g000001.1.cds

Sequence Viewer

Length: 573 bp
ctgattgtggctctcctagtcgccatcgccggcacggccctctacgtcctctaccgtcctcaccggcccgagttcaccgtcacagccctcaaaatcgggaagctcaacctcaccgacaaccccgacggcgccagctcacacctcgtcacacgcttcaacctcactatcgctcccaaaaacccgaacaaccacctcagcttcgcctacgactccttcgcgctcactctctccgccgccaacaacgacgtcgttctcggaaacggctcgatcccggccttcttcagcgacacgaagaacttcacgtcgttccggtcgatcatcaccacggcgagtgacctcgatttggagtccgtcaagtctctgagatcggatctgaagcggaagggcggggttccgatgaagttgcggatggacaccaaggtgaaggtgacggtgggagggaaattgaagagcaacaaggtggggattagggttacgtgtcagccaatcagaggggtagcaccgaaaggtaagtctccgtcggtggcttctgttgctgactcccattgcaaggttgatcttcggatcaagatc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

191

Amino Acids

20.53

Weight (kDa)

10.09

Isoelectric Point (pI)

17.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 249
AccB1I GGYRCC 1 cut(s) 128
AccII CGCG 1 cut(s) 218
AciI CCGC 5 cut(s) 231, 234, 379, 387, 406
AclWI GGATC 3 cut(s) 262, 378, 572
AcuI CTGAAG 2 cut(s) 265, 395
AcyI GRCGYC 2 cut(s) 129, 246
AfiI CCNNNNNNNGG 3 cut(s) 343, 491, 550
AflIII ACRYGT 1 cut(s) 476
AgsI TTSAA 2 cut(s) 157, 448
AjiI CACGTC 1 cut(s) 303
AleI CACNNNNGTG 1 cut(s) 419
AluBI AGCT 3 cut(s) 103, 135, 198
AluI AGCT 3 cut(s) 103, 135, 198
Alw26I GTCTC 2 cut(s) 363, 519
AlwI GGATC 3 cut(s) 262, 378, 572
Ama87I CYCGRG 1 cut(s) 68
AoxI GGCC 3 cut(s) 36, 65, 273
ArsI GACNNNNNNTTYG 2 cut(s) 497, 529
Asp700I GAANNNNTTC 1 cut(s) 296
AspLEI GCGC 2 cut(s) 131, 220
AspS9I GGNCC 2 cut(s) 37, 66
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 6 cut(s) 53, 67, 103, 313, 433, 439
AvaI CYCGRG 1 cut(s) 68
BanI GGYRCC 1 cut(s) 128
BbvCI CCTCAGC 1 cut(s) 194
BccI CCATC 2 cut(s) 32, 403
BceAI ACGGC 4 cut(s) 51, 142, 277, 342
BcgI CGANNNNNNTGC 2 cut(s) 385, 419
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 2 cut(s) 363, 519
BfaI CTAG 1 cut(s) 17
BfoI RGCGCY 1 cut(s) 132
BglI GCCNNNNNGGC 1 cut(s) 35
BisI GCNGC 1 cut(s) 234
BlsI GCNGC 1 cut(s) 235
Bme1390I CCNGG 1 cut(s) 272
BmeT110I CYCGRG 1 cut(s) 68
BmgBI CACGTC 1 cut(s) 303
BmgT120I GGNCC 2 cut(s) 37, 66
BmiI GGNNCC 2 cut(s) 130, 393
BmrFI CCNGG 1 cut(s) 272
Bpu10I CCTNAGC 1 cut(s) 194
BpuMI CCSGG 1 cut(s) 272
BsaAI YACGTR 1 cut(s) 477
BsaBI GATNNNNATC 1 cut(s) 569
BsaHI GRCGYC 2 cut(s) 129, 246
BsaJI CCNNGG 2 cut(s) 324, 417
BsaWI WCCGGW 1 cut(s) 309
BsaXI ACNNNNNCTCC 2 cut(s) 154, 184
Bsc4I CCNNNNNNNGG 3 cut(s) 343, 491, 550
Bse118I RCCGGY 2 cut(s) 29, 63
Bse3DI GCAATG 1 cut(s) 544
Bse8I GATNNNNATC 1 cut(s) 569
BseDI CCNNGG 2 cut(s) 324, 417
BseGI GGATG 1 cut(s) 414
BseJI GATNNNNATC 1 cut(s) 569
BseLI CCNNNNNNNGG 3 cut(s) 343, 491, 550
BseMI GCAATG 1 cut(s) 544
BseMII CTCAG 2 cut(s) 208, 353
Bsh1236I CGCG 1 cut(s) 218
Bsh1285I CGRYCG 1 cut(s) 314
BshFI GGCC 3 cut(s) 38, 67, 275
BshNI GGYRCC 1 cut(s) 128
BsiEI CGRYCG 1 cut(s) 314
BsiHKCI CYCGRG 1 cut(s) 68
BsiSI CCGG 4 cut(s) 30, 64, 272, 310
BslI CCNNNNNNNGG 3 cut(s) 343, 491, 550
BsmAI GTCTC 2 cut(s) 363, 519
BsnI GGCC 3 cut(s) 38, 67, 275
BsoBI CYCGRG 1 cut(s) 68
Bsp143I GATC 6 cut(s) 267, 315, 365, 370, 556, 564
BspACI CCGC 5 cut(s) 231, 234, 379, 387, 406
BspANI GGCC 3 cut(s) 38, 67, 275
BspCNI CTCAG 2 cut(s) 207, 354
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 2 cut(s) 130, 393
BspPI GGATC 3 cut(s) 262, 378, 572
BspQI GCTCTTC 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 544
BsrFI RCCGGY 2 cut(s) 29, 63
BssAI RCCGGY 2 cut(s) 29, 63
BssECI CCNNGG 2 cut(s) 324, 417
BssMI GATC 6 cut(s) 267, 315, 365, 370, 556, 564
BssNI GRCGYC 2 cut(s) 129, 246
BssT1I CCWWGG 1 cut(s) 417
Bst4CI ACNGT 3 cut(s) 56, 79, 433
Bst6I CTCTTC 1 cut(s) 443
BstACI GRCGYC 2 cut(s) 129, 246
BstBAI YACGTR 1 cut(s) 477
BstC8I GCNNGC 2 cut(s) 31, 133
BstDEI CTNAG 2 cut(s) 194, 362
BstDSI CCRYGG 1 cut(s) 324
BstF5I GGATG 1 cut(s) 414
BstFNI CGCG 1 cut(s) 218
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 2 cut(s) 131, 220
BstKTI GATC 7 cut(s) 270, 318, 368, 373, 559, 567, 573
BstMAI GTCTC 2 cut(s) 363, 519
BstMBI GATC 6 cut(s) 267, 315, 365, 370, 556, 564
BstMCI CGRYCG 1 cut(s) 314
BstMWI GCNNNNNNNGC 2 cut(s) 35, 533
BstSCI CCNGG 1 cut(s) 270
BstUI CGCG 1 cut(s) 218
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BsuRI GGCC 3 cut(s) 38, 67, 275
BtgI CCRYGG 1 cut(s) 324
BtgZI GCGATG 1 cut(s) 10
BtrI CACGTC 1 cut(s) 303
BtsCI GGATG 1 cut(s) 414
Cac8I GCNNGC 2 cut(s) 31, 133
CfoI GCGC 2 cut(s) 131, 220
Cfr10I RCCGGY 2 cut(s) 29, 63
Cfr13I GGNCC 2 cut(s) 37, 66
DdeI CTNAG 2 cut(s) 194, 362
DinI GGCGCC 1 cut(s) 130
DpnI GATC 7 cut(s) 269, 317, 367, 372, 558, 566, 572
DpnII GATC 6 cut(s) 267, 315, 365, 370, 556, 564
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
EciI GGCGGA 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 417
Eco57I CTGAAG 2 cut(s) 265, 395
Eco88I CYCGRG 1 cut(s) 68
EcoT14I CCWWGG 1 cut(s) 417
EgeI GGCGCC 1 cut(s) 130
EheI GGCGCC 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 417
FauI CCCGC 1 cut(s) 380
Fnu4HI GCNGC 1 cut(s) 234
FokI GGATG 1 cut(s) 421
Fsp4HI GCNGC 1 cut(s) 234
FspBI CTAG 1 cut(s) 17
GlaI GCGC 2 cut(s) 130, 219
GluI GCNGC 1 cut(s) 234
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 3 cut(s) 38, 67, 275
HapII CCGG 4 cut(s) 30, 64, 272, 310
HhaI GCGC 2 cut(s) 131, 220
Hin1I GRCGYC 2 cut(s) 129, 246
Hin6I GCGC 2 cut(s) 129, 218
HinP1I GCGC 2 cut(s) 129, 218
HinfI GANTC 3 cut(s) 209, 347, 539
HpaII CCGG 4 cut(s) 30, 64, 272, 310
HphI GGTGA 6 cut(s) 53, 67, 103, 313, 433, 439
Hpy166II GTNNAC 1 cut(s) 75
Hpy188I TCNGA 7 cut(s) 257, 363, 370, 375, 396, 491, 564
Hpy188III TCNNGA 2 cut(s) 97, 568
Hpy8I GTNNAC 1 cut(s) 75
Hpy99I CGWCG 5 cut(s) 128, 248, 251, 307, 523
HpyAV CCTTC 4 cut(s) 223, 286, 376, 418
HpyCH4III ACNGT 3 cut(s) 56, 79, 433
HpyCH4IV ACGT 4 cut(s) 45, 246, 302, 476
HpyCH4V TGCA 1 cut(s) 549
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 533
HpyF3I CTNAG 2 cut(s) 194, 362
HpySE526I ACGT 4 cut(s) 45, 246, 302, 476
Hsp92I GRCGYC 2 cut(s) 129, 246
HspAI GCGC 2 cut(s) 129, 218
KasI GGCGCC 1 cut(s) 128
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 6 cut(s) 267, 315, 365, 370, 556, 564
LguI GCTCTTC 1 cut(s) 443
LmnI GCTCC 1 cut(s) 175
LpnPI CCDG 5 cut(s) 43, 77, 145, 285, 323
MaeI CTAG 1 cut(s) 17
MaeII ACGT 4 cut(s) 45, 246, 302, 476
MaeIII GTNAC 5 cut(s) 79, 145, 332, 427, 472
MalI GATC 7 cut(s) 269, 317, 367, 372, 558, 566, 572
MboI GATC 6 cut(s) 267, 315, 365, 370, 556, 564
MboII GAAGA 4 cut(s) 271, 304, 460, 551
MflI RGATCY 1 cut(s) 370
MluCI AATT 1 cut(s) 443
Mly113I GGCGCC 1 cut(s) 129
MlyI GAGTC 3 cut(s) 203, 356, 533
MroNI GCCGGC 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 296
MslI CAYNNNNRTG 1 cut(s) 419
MspI CCGG 4 cut(s) 30, 64, 272, 310
MspR9I CCNGG 1 cut(s) 272
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 2 cut(s) 35, 533
NaeI GCCGGC 1 cut(s) 31
NarI GGCGCC 1 cut(s) 129
NciI CCSGG 1 cut(s) 272
NdeII GATC 6 cut(s) 267, 315, 365, 370, 556, 564
NgoMIV GCCGGC 1 cut(s) 29
NlaIV GGNNCC 2 cut(s) 130, 393
NmuCI GTSAC 4 cut(s) 79, 145, 332, 427
OliI CACNNNNGTG 1 cut(s) 419
PciSI GCTCTTC 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 311
PdiI GCCGGC 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 296
PkrI GCNGC 1 cut(s) 235
PleI GAGTC 3 cut(s) 203, 355, 533
PluTI GGCGCC 1 cut(s) 132
PpsI GAGTC 3 cut(s) 203, 355, 533
Ppu21I YACGTR 1 cut(s) 477
PspN4I GGNNCC 2 cut(s) 130, 393
PspPI GGNCC 2 cut(s) 37, 66
PsuI RGATCY 1 cut(s) 370
RseI CAYNNNNRTG 1 cut(s) 419
SapI GCTCTTC 1 cut(s) 443
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 6 cut(s) 267, 315, 365, 370, 556, 564
Sau96I GGNCC 2 cut(s) 37, 66
SchI GAGTC 3 cut(s) 203, 356, 533
ScrFI CCNGG 1 cut(s) 272
SfoI GGCGCC 1 cut(s) 130
SmiMI CAYNNNNRTG 1 cut(s) 419
Sse9I AATT 1 cut(s) 443
SsiI CCGC 5 cut(s) 231, 234, 379, 387, 406
SspDI GGCGCC 1 cut(s) 128
SspMI CTAG 1 cut(s) 17
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 1 cut(s) 417
TaaI ACNGT 3 cut(s) 56, 79, 433
TaiI ACGT 4 cut(s) 48, 249, 305, 479
TaqI TCGA 3 cut(s) 266, 314, 339
TasI AATT 1 cut(s) 443
TauI GCSGC 1 cut(s) 236
TseFI GTSAC 4 cut(s) 79, 145, 332, 427
Tsp45I GTSAC 4 cut(s) 79, 145, 332, 427
TspDTI ATGAA 1 cut(s) 413
TspGWI ACGGA 2 cut(s) 340, 507
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 1 cut(s) 17
ZraI GACGTC 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.