pycom08g05180

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011853
Physical Location & Seq
Forward (+)
3989292 .. 3990076
785 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05180.1

Sequence Viewer

Length: 717 bp
ATGACAGACCGAGTTTACCCATCTTCCAAACCCACCGCCAATGGCGGCACCGCCGCCGCACCCGCCGCCTCACCCGCCGCCGCAACCACTGCCGGTACCACAGCCAACCCACCAAGTACCAAACCCCAACCCAGACAGCCATACCGCCCGAAACCCCAGTATCACAACCGTCGCCACCGCCGGAGCAGCCGCAATTTATGCTGTTGCTGCTGCTTCTGGTCCTTCCTCATCCTCATAGCCCTCGCCCTCCTCGCCGCCATAGCTGGAGCCGCGGTCTACATCCTCTACCGCCCCCACCGCCCCGAGTTCACCCTCACGTCCGTCCGCATTGCGAAGTTCAACCTCACCACCACCGCCGACTTCTCGACCTCCCACCTCACCACCCTCTTCAACCTCACCCTCACCTCCGAAAACCCCAACAACCACCTCACCTTCTCCTACGATCCCTTCAATCTCGTCCTCTCCACCACCAGAGACGTCCAAGTCGCAAACGGGTCGATCCCGGCGTTCACGAGCGCCACGAAGAACAGCACCTTCTTCCGCTCCGTACTGTCGACGTCGCAGGATCTCGACGTCGAGTCGGTGAAGTCGCTGAGATCGGATCTGAGGAAGCAGAGCGGGGTGGCGCTGAAGCTGCAGGTGGACACCGAAGTGAAGGTGTCGATGGGGAAGCTGAGGAGCAAGAAGGTGGGGATTAGGGTTACGTTGCAACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

239

Amino Acids

26.06

Weight (kDa)

10.29

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 133 - 235 2.6e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 628
AasI GACNNNNNNGTC 1 cut(s) 482
AatII GACGTC 3 cut(s) 480, 560, 576
Acc36I ACCTGC 1 cut(s) 628
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 2 cut(s) 47, 95
AccBSI CCGCTC 2 cut(s) 543, 618
AccI GTMKAC 2 cut(s) 276, 554
AccII CGCG 1 cut(s) 272
AclI AACGTT 1 cut(s) 712
AclWI GGATC 4 cut(s) 437, 493, 573, 609
AcuI CTGAAG 1 cut(s) 650
AcyI GRCGYC 3 cut(s) 477, 557, 573
AfaI GTAC 3 cut(s) 97, 118, 549
AgsI TTSAA 3 cut(s) 340, 391, 451
AhdI GACNNNNNGTC 1 cut(s) 577
AjiI CACGTC 1 cut(s) 318
AleI CACNNNNGTG 1 cut(s) 650
AluBI AGCT 3 cut(s) 263, 634, 673
AluI AGCT 3 cut(s) 263, 634, 673
Alw26I GTCTC 1 cut(s) 468
AlwI GGATC 4 cut(s) 437, 493, 573, 609
Ama87I CYCGRG 1 cut(s) 302
ApeKI GCWGC 4 cut(s) 186, 207, 210, 634
Asp718I GGTACC 1 cut(s) 95
AspLEI GCGC 2 cut(s) 518, 628
AspS9I GGNCC 1 cut(s) 219
AsuC2I CCSGG 1 cut(s) 503
AsuHPI GGTGA 8 cut(s) 63, 301, 337, 370, 388, 394, 421, 595
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 1 cut(s) 219
BaeI ACNNNNGTAYC 2 cut(s) 143, 176
BanI GGYRCC 2 cut(s) 47, 95
BauI CACGAG 1 cut(s) 511
BbvCI CCTCAGC 1 cut(s) 674
BbvI GCAGC 4 cut(s) 194, 197, 198, 621
BccI CCATC 2 cut(s) 28, 658
BcgI CGANNNNNNTGC 2 cut(s) 477, 511
BcnI CCSGG 1 cut(s) 503
BcoDI GTCTC 1 cut(s) 468
BfmI CTRYAG 1 cut(s) 635
BfoI RGCGCY 2 cut(s) 519, 629
BfuAI ACCTGC 1 cut(s) 628
Bme1390I CCNGG 1 cut(s) 503
Bme18I GGWCC 1 cut(s) 219
BmeRI GACNNNNNGTC 1 cut(s) 577
BmeT110I CYCGRG 1 cut(s) 302
BmgBI CACGTC 1 cut(s) 318
BmgT120I GGNCC 1 cut(s) 219
BmiI GGNNCC 3 cut(s) 49, 97, 268
BmrFI CCNGG 1 cut(s) 503
BmrI ACTGGG 1 cut(s) 151
BmuI ACTGGG 1 cut(s) 151
BpmI CTGGAG 1 cut(s) 285
Bpu10I CCTNAGC 1 cut(s) 674
BpuMI CCSGG 1 cut(s) 503
BsaHI GRCGYC 3 cut(s) 477, 557, 573
BsaJI CCNNGG 1 cut(s) 270
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 1 cut(s) 157
Bse3DI GCAATG 1 cut(s) 327
BseDI CCNNGG 1 cut(s) 270
BseGI GGATG 2 cut(s) 228, 279
BseMI GCAATG 1 cut(s) 327
BseMII CTCAG 3 cut(s) 584, 596, 665
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 2 cut(s) 239, 691
BseXI GCAGC 4 cut(s) 194, 197, 198, 621
Bsh1236I CGCG 1 cut(s) 272
BshNI GGYRCC 2 cut(s) 47, 95
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 3 cut(s) 93, 181, 503
BsmAI GTCTC 1 cut(s) 468
BsmBI CGTCTC 1 cut(s) 468
BsoBI CYCGRG 1 cut(s) 302
Bsp143I GATC 5 cut(s) 442, 498, 565, 596, 601
BspCNI CTCAG 3 cut(s) 585, 597, 666
BspFNI CGCG 1 cut(s) 272
BspLI GGNNCC 3 cut(s) 49, 97, 268
BspMAI CTGCAG 1 cut(s) 639
BspMI ACCTGC 1 cut(s) 628
BspPI GGATC 4 cut(s) 437, 493, 573, 609
BspT107I GGYRCC 2 cut(s) 47, 95
BsrBI CCGCTC 2 cut(s) 543, 618
BsrDI GCAATG 1 cut(s) 327
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 1 cut(s) 157
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 270
BssMI GATC 5 cut(s) 442, 498, 565, 596, 601
BssNI GRCGYC 3 cut(s) 477, 557, 573
BssSI CACGAG 1 cut(s) 511
Bst2BI CACGAG 1 cut(s) 511
Bst4CI ACNGT 2 cut(s) 170, 552
Bst6I CTCTTC 1 cut(s) 392
BstACI GRCGYC 3 cut(s) 477, 557, 573
BstAPI GCANNNNNTGC 2 cut(s) 89, 198
BstDEI CTNAG 3 cut(s) 593, 605, 674
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 2 cut(s) 228, 279
BstFNI CGCG 1 cut(s) 272
BstH2I RGCGCY 2 cut(s) 519, 629
BstHHI GCGC 2 cut(s) 518, 628
BstKTI GATC 5 cut(s) 445, 501, 568, 599, 604
BstMAI GTCTC 1 cut(s) 468
BstMBI GATC 5 cut(s) 442, 498, 565, 596, 601
BstSCI CCNGG 1 cut(s) 501
BstSFI CTRYAG 1 cut(s) 635
BstUI CGCG 1 cut(s) 272
BstV1I GCAGC 4 cut(s) 194, 197, 198, 621
BstX2I RGATCY 2 cut(s) 565, 601
BstYI RGATCY 2 cut(s) 565, 601
BtgI CCRYGG 1 cut(s) 270
BtrI CACGTC 1 cut(s) 318
BtsCI GGATG 2 cut(s) 228, 279
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 87
BveI ACCTGC 1 cut(s) 628
CfoI GCGC 2 cut(s) 518, 628
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 219
Cfr42I CCGCGG 1 cut(s) 273
Csp6I GTAC 3 cut(s) 96, 117, 548
CviJI RGCY 8 cut(s) 104, 139, 189, 239, 263, 269, 634, 673
CviKI_1 RGCY 8 cut(s) 104, 139, 189, 239, 263, 269, 634, 673
CviQI GTAC 3 cut(s) 96, 117, 548
DdeI CTNAG 3 cut(s) 593, 605, 674
DpnI GATC 5 cut(s) 444, 500, 567, 598, 603
DpnII GATC 5 cut(s) 442, 498, 565, 596, 601
DrdI GACNNNNNNGTC 1 cut(s) 482
DriI GACNNNNNGTC 1 cut(s) 577
DseDI GACNNNNNNGTC 1 cut(s) 482
Eam1104I CTCTTC 1 cut(s) 392
Eam1105I GACNNNNNGTC 1 cut(s) 577
EarI CTCTTC 1 cut(s) 392
Eco47I GGWCC 1 cut(s) 219
Eco57I CTGAAG 1 cut(s) 650
Eco88I CYCGRG 1 cut(s) 302
Esp3I CGTCTC 1 cut(s) 468
FaiI YATR 4 cut(s) 142, 199, 236, 260
FauI CCCGC 3 cut(s) 70, 82, 611
FblI GTMKAC 2 cut(s) 276, 554
FokI GGATG 2 cut(s) 215, 266
GlaI GCGC 2 cut(s) 517, 627
GsuI CTGGAG 1 cut(s) 285
HaeII RGCGCY 2 cut(s) 519, 629
HapII CCGG 3 cut(s) 93, 181, 503
HhaI GCGC 2 cut(s) 518, 628
Hin1I GRCGYC 3 cut(s) 477, 557, 573
Hin6I GCGC 2 cut(s) 516, 626
HinP1I GCGC 2 cut(s) 516, 626
HincII GTYRAC 1 cut(s) 555
HindII GTYRAC 1 cut(s) 555
HinfI GANTC 1 cut(s) 578
HpaII CCGG 3 cut(s) 93, 181, 503
HphI GGTGA 8 cut(s) 63, 301, 337, 370, 388, 394, 421, 595
Hpy166II GTNNAC 6 cut(s) 16, 277, 309, 510, 555, 643
Hpy188I TCNGA 3 cut(s) 409, 601, 606
Hpy188III TCNNGA 3 cut(s) 364, 511, 569
Hpy8I GTNNAC 6 cut(s) 16, 277, 309, 510, 555, 643
Hpy99I CGWCG 5 cut(s) 174, 559, 562, 575, 578
HpyAV CCTTC 6 cut(s) 232, 442, 457, 544, 649, 679
HpyCH4III ACNGT 2 cut(s) 170, 552
HpyCH4IV ACGT 6 cut(s) 317, 477, 557, 573, 704, 712
HpyCH4V TGCA 2 cut(s) 637, 709
HpyF3I CTNAG 3 cut(s) 593, 605, 674
HpySE526I ACGT 6 cut(s) 317, 477, 557, 573, 704, 712
Hsp92I GRCGYC 3 cut(s) 477, 557, 573
HspAI GCGC 2 cut(s) 516, 626
KpnI GGTACC 1 cut(s) 99
KspI CCGCGG 1 cut(s) 273
Kzo9I GATC 5 cut(s) 442, 498, 565, 596, 601
LmnI GCTCC 4 cut(s) 183, 266, 548, 678
Lsp1109I GCAGC 4 cut(s) 194, 197, 198, 621
MaeII ACGT 6 cut(s) 317, 477, 557, 573, 704, 712
MaeIII GTNAC 1 cut(s) 700
MalI GATC 5 cut(s) 444, 500, 567, 598, 603
MbiI CCGCTC 2 cut(s) 543, 618
MboI GATC 5 cut(s) 442, 498, 565, 596, 601
MboII GAAGA 4 cut(s) 15, 379, 529, 535
MflI RGATCY 2 cut(s) 565, 601
MluCI AATT 1 cut(s) 193
MlyI GAGTC 1 cut(s) 587
MslI CAYNNNNRTG 1 cut(s) 650
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 3 cut(s) 93, 181, 503
MspR9I CCNGG 1 cut(s) 503
MvnI CGCG 1 cut(s) 272
NciI CCSGG 1 cut(s) 503
NdeII GATC 5 cut(s) 442, 498, 565, 596, 601
NlaIV GGNNCC 3 cut(s) 49, 97, 268
OliI CACNNNNGTG 1 cut(s) 650
PaqCI CACCTGC 1 cut(s) 628
PcsI WCGNNNNNNNCGW 2 cut(s) 503, 518
PleI GAGTC 1 cut(s) 586
PpsI GAGTC 1 cut(s) 586
Psp1406I AACGTT 1 cut(s) 712
PspN4I GGNNCC 3 cut(s) 49, 97, 268
PspPI GGNCC 1 cut(s) 219
PstI CTGCAG 1 cut(s) 639
PsuI RGATCY 2 cut(s) 565, 601
RsaI GTAC 3 cut(s) 97, 118, 549
RsaNI GTAC 3 cut(s) 96, 117, 548
RseI CAYNNNNRTG 1 cut(s) 650
SacII CCGCGG 1 cut(s) 273
SalI GTCGAC 1 cut(s) 553
Sau3AI GATC 5 cut(s) 442, 498, 565, 596, 601
Sau96I GGNCC 1 cut(s) 219
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 1 cut(s) 503
SfcI CTRYAG 1 cut(s) 635
Sfr303I CCGCGG 1 cut(s) 273
SgrBI CCGCGG 1 cut(s) 273
SinI GGWCC 1 cut(s) 219
SmiMI CAYNNNNRTG 1 cut(s) 650
Sse9I AATT 1 cut(s) 193
StyD4I CCNGG 1 cut(s) 501
TaaI ACNGT 2 cut(s) 170, 552
TaiI ACGT 6 cut(s) 320, 480, 560, 576, 707, 715
TaqI TCGA 6 cut(s) 365, 497, 554, 570, 576, 662
TaqII GACCGA 1 cut(s) 24
TasI AATT 1 cut(s) 193
TauI GCSGC 9 cut(s) 48, 56, 59, 68, 80, 83, 192, 257, 272
TscAI CASTG 1 cut(s) 94
TseI GCWGC 4 cut(s) 186, 207, 210, 634
TspGWI ACGGA 2 cut(s) 310, 535
TspRI CASTG 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 219
XmiI GTMKAC 2 cut(s) 276, 554
ZraI GACGTC 3 cut(s) 478, 558, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.