MD08G1062800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011853
Physical Location & Seq
Forward (+)
4951457 .. 4952272
816 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1062800.v1.1.491

Sequence Viewer

Length: 816 bp
ATGACAGACCGAGTCTACCCATCTTCCAAACCCAATGCCAACGGCGGCGCCGCCGCAGCACCCGCCGCACCCGCCGCCGCAACAACCGCCGGTACCACAGCCAACCCACCAAGTACCAAACCCCAACTCAGACAGCCATACCGCCCGAAACCCCAGTACCACAACCGTCACCACCGCCGGAGCAGCCGCAATTTATGCTGTTGCTGCTGCTTCTGGTCCTTCCTCATCCTCATAGCCCTCGCCCTCCTCGCCGCCATAGCTGGCGCCGCGGTCTACATCCTCTACCGCCCCCACCGCCCCGAGTTCACCCTCACGTCCGTCCGCATTGCGAAGCTCAACCTCACCACCACCGCCGACTTCTCGACATCCCACCTCGCCACCCTCTTCAACCTCACCCTCACCTCCGAAAACCCCAACAACCACCTCACCTTCTCCTACGATCCCTTCACTCTCTTACTCTCCACCACCAGAGACGTCCAAGTCGCAAACGGGTCGATCCCGGCGTTCACGAGCGCCACGAAGAACAGCACCTTCTTCCGCTCCGTACTGTCAACGTCGCAGGATCTCGACGTCGAGTCGGTGAAGTCGCTTAGATCGGATCTGAGGAAGCAGAGCGGGGTGGCGCTGAAGCTGCAGATAGACACCGAAGTGAAGCTGTCGATGGGGAAGCTGAGGAGCAAGAAGGTGGGGATTAGGGTTACGTGTGAAGGAATCAAAGGGGTTGTACCAAAAGGTAAGACACCGTCGGTGGCTTCCGTTTCCAACTCCAAGTGCAACGTTGATCTTCGGATCAAGATCTGGAAATGGACCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

272

Amino Acids

29.58

Weight (kDa)

10.1

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 132 - 235 3.1e-07 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 479
AatII GACGTC 2 cut(s) 477, 573
Acc65I GGTACC 1 cut(s) 92
AccB1I GGYRCC 3 cut(s) 47, 92, 263
AccBSI CCGCTC 2 cut(s) 540, 615
AccI GTMKAC 2 cut(s) 15, 273
AccII CGCG 1 cut(s) 269
AclI AACGTT 1 cut(s) 777
AclWI GGATC 5 cut(s) 434, 490, 570, 606, 797
AcuI CTGAAG 1 cut(s) 647
AcyI GRCGYC 4 cut(s) 48, 264, 474, 570
AfaI GTAC 5 cut(s) 94, 115, 158, 546, 726
AflIII ACRYGT 1 cut(s) 701
AgsI TTSAA 1 cut(s) 388
AhdI GACNNNNNGTC 1 cut(s) 574
AjiI CACGTC 1 cut(s) 315
AleI CACNNNNGTG 1 cut(s) 647
AluBI AGCT 5 cut(s) 260, 334, 631, 655, 670
AluI AGCT 5 cut(s) 260, 334, 631, 655, 670
Alw26I GTCTC 1 cut(s) 465
AlwI GGATC 5 cut(s) 434, 490, 570, 606, 797
Ama87I CYCGRG 1 cut(s) 299
ApeKI GCWGC 5 cut(s) 56, 183, 204, 207, 631
Asp718I GGTACC 1 cut(s) 92
AspLEI GCGC 4 cut(s) 50, 266, 515, 625
AspS9I GGNCC 2 cut(s) 216, 807
AsuC2I CCSGG 1 cut(s) 500
AsuHPI GGTGA 7 cut(s) 161, 298, 334, 385, 391, 418, 592
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 2 cut(s) 216, 807
BaeI ACNNNNGTAYC 2 cut(s) 140, 173
BanI GGYRCC 3 cut(s) 47, 92, 263
BauI CACGAG 1 cut(s) 508
BbvCI CCTCAGC 1 cut(s) 671
BbvI GCAGC 5 cut(s) 68, 191, 194, 195, 618
BccI CCATC 2 cut(s) 28, 655
BceAI ACGGC 1 cut(s) 58
BcgI CGANNNNNNTGC 2 cut(s) 474, 508
BcnI CCSGG 1 cut(s) 500
BcoDI GTCTC 1 cut(s) 465
BfmI CTRYAG 1 cut(s) 632
BfoI RGCGCY 4 cut(s) 51, 267, 516, 626
BglII AGATCT 1 cut(s) 795
Bme1390I CCNGG 1 cut(s) 500
Bme18I GGWCC 2 cut(s) 216, 807
BmeRI GACNNNNNGTC 1 cut(s) 574
BmeT110I CYCGRG 1 cut(s) 299
BmgBI CACGTC 1 cut(s) 315
BmgT120I GGNCC 2 cut(s) 216, 807
BmiI GGNNCC 3 cut(s) 49, 94, 265
BmrFI CCNGG 1 cut(s) 500
BmrI ACTGGG 1 cut(s) 148
BmuI ACTGGG 1 cut(s) 148
Bpu10I CCTNAGC 1 cut(s) 671
BpuMI CCSGG 1 cut(s) 500
BsaAI YACGTR 1 cut(s) 702
BsaBI GATNNNNATC 1 cut(s) 794
BsaHI GRCGYC 4 cut(s) 48, 264, 474, 570
BsaJI CCNNGG 1 cut(s) 267
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 1 cut(s) 154
Bse3DI GCAATG 1 cut(s) 324
Bse8I GATNNNNATC 1 cut(s) 794
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 3 cut(s) 225, 276, 365
BseJI GATNNNNATC 1 cut(s) 794
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 3 cut(s) 142, 593, 662
BseNI ACTGG 1 cut(s) 154
BseRI GAGGAG 2 cut(s) 236, 688
BseXI GCAGC 5 cut(s) 68, 191, 194, 195, 618
Bsh1236I CGCG 1 cut(s) 269
BshNI GGYRCC 3 cut(s) 47, 92, 263
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 3 cut(s) 90, 178, 500
BsmAI GTCTC 1 cut(s) 465
BsmBI CGTCTC 1 cut(s) 465
BsoBI CYCGRG 1 cut(s) 299
Bsp143I GATC 8 cut(s) 439, 495, 562, 593, 598, 781, 789, 795
BspCNI CTCAG 3 cut(s) 141, 594, 663
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 3 cut(s) 49, 94, 265
BspMAI CTGCAG 1 cut(s) 636
BspPI GGATC 5 cut(s) 434, 490, 570, 606, 797
BspT107I GGYRCC 3 cut(s) 47, 92, 263
BsrBI CCGCTC 2 cut(s) 540, 615
BsrDI GCAATG 1 cut(s) 324
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 1 cut(s) 154
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 8 cut(s) 439, 495, 562, 593, 598, 781, 789, 795
BssNI GRCGYC 4 cut(s) 48, 264, 474, 570
BssSI CACGAG 1 cut(s) 508
Bst2BI CACGAG 1 cut(s) 508
Bst4CI ACNGT 3 cut(s) 167, 549, 744
Bst6I CTCTTC 1 cut(s) 389
BstACI GRCGYC 4 cut(s) 48, 264, 474, 570
BstAPI GCANNNNNTGC 1 cut(s) 195
BstBAI YACGTR 1 cut(s) 702
BstC8I GCNNGC 1 cut(s) 262
BstDEI CTNAG 4 cut(s) 128, 590, 602, 671
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 3 cut(s) 225, 276, 365
BstFNI CGCG 1 cut(s) 269
BstH2I RGCGCY 4 cut(s) 51, 267, 516, 626
BstHHI GCGC 4 cut(s) 50, 266, 515, 625
BstKTI GATC 8 cut(s) 442, 498, 565, 596, 601, 784, 792, 798
BstMAI GTCTC 1 cut(s) 465
BstMBI GATC 8 cut(s) 439, 495, 562, 593, 598, 781, 789, 795
BstSCI CCNGG 1 cut(s) 498
BstSFI CTRYAG 1 cut(s) 632
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 5 cut(s) 68, 191, 194, 195, 618
BstX2I RGATCY 3 cut(s) 562, 598, 795
BstYI RGATCY 3 cut(s) 562, 598, 795
BtgI CCRYGG 1 cut(s) 267
BtrI CACGTC 1 cut(s) 315
BtsCI GGATG 3 cut(s) 225, 276, 365
Cac8I GCNNGC 1 cut(s) 262
CfoI GCGC 4 cut(s) 50, 266, 515, 625
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 2 cut(s) 216, 807
Cfr42I CCGCGG 1 cut(s) 270
Csp6I GTAC 5 cut(s) 93, 114, 157, 545, 725
CviQI GTAC 5 cut(s) 93, 114, 157, 545, 725
DdeI CTNAG 4 cut(s) 128, 590, 602, 671
DinI GGCGCC 2 cut(s) 49, 265
DpnI GATC 8 cut(s) 441, 497, 564, 595, 600, 783, 791, 797
DpnII GATC 8 cut(s) 439, 495, 562, 593, 598, 781, 789, 795
DrdI GACNNNNNNGTC 1 cut(s) 479
DriI GACNNNNNGTC 1 cut(s) 574
DseDI GACNNNNNNGTC 1 cut(s) 479
Eam1104I CTCTTC 1 cut(s) 389
Eam1105I GACNNNNNGTC 1 cut(s) 574
EarI CTCTTC 1 cut(s) 389
Eco47I GGWCC 2 cut(s) 216, 807
Eco57I CTGAAG 1 cut(s) 647
Eco88I CYCGRG 1 cut(s) 299
EgeI GGCGCC 2 cut(s) 49, 265
EheI GGCGCC 2 cut(s) 49, 265
Esp3I CGTCTC 1 cut(s) 465
FaiI YATR 4 cut(s) 139, 196, 233, 257
FauI CCCGC 3 cut(s) 70, 79, 608
FblI GTMKAC 2 cut(s) 15, 273
FokI GGATG 3 cut(s) 212, 263, 352
GlaI GCGC 4 cut(s) 49, 265, 514, 624
HaeII RGCGCY 4 cut(s) 51, 267, 516, 626
HapII CCGG 3 cut(s) 90, 178, 500
HhaI GCGC 4 cut(s) 50, 266, 515, 625
Hin1I GRCGYC 4 cut(s) 48, 264, 474, 570
Hin6I GCGC 4 cut(s) 48, 264, 513, 623
HinP1I GCGC 4 cut(s) 48, 264, 513, 623
HincII GTYRAC 1 cut(s) 552
HindII GTYRAC 1 cut(s) 552
HinfI GANTC 3 cut(s) 12, 575, 711
HpaII CCGG 3 cut(s) 90, 178, 500
HphI GGTGA 7 cut(s) 161, 298, 334, 385, 391, 418, 592
Hpy166II GTNNAC 5 cut(s) 16, 274, 306, 507, 552
Hpy188I TCNGA 5 cut(s) 131, 406, 598, 603, 789
Hpy188III TCNNGA 5 cut(s) 361, 508, 566, 793, 799
Hpy8I GTNNAC 5 cut(s) 16, 274, 306, 507, 552
Hpy99I CGWCG 4 cut(s) 559, 572, 575, 748
HpyAV CCTTC 6 cut(s) 229, 439, 454, 541, 676, 701
HpyCH4III ACNGT 3 cut(s) 167, 549, 744
HpyCH4IV ACGT 6 cut(s) 314, 474, 554, 570, 701, 777
HpyCH4V TGCA 2 cut(s) 634, 774
HpyF3I CTNAG 4 cut(s) 128, 590, 602, 671
HpySE526I ACGT 6 cut(s) 314, 474, 554, 570, 701, 777
Hsp92I GRCGYC 4 cut(s) 48, 264, 474, 570
HspAI GCGC 4 cut(s) 48, 264, 513, 623
KasI GGCGCC 2 cut(s) 47, 263
KpnI GGTACC 1 cut(s) 96
KspI CCGCGG 1 cut(s) 270
Kzo9I GATC 8 cut(s) 439, 495, 562, 593, 598, 781, 789, 795
LmnI GCTCC 3 cut(s) 180, 545, 675
LpnPI CCDG 9 cut(s) 103, 167, 191, 199, 246, 481, 513, 545, 784
Lsp1109I GCAGC 5 cut(s) 68, 191, 194, 195, 618
MaeII ACGT 6 cut(s) 314, 474, 554, 570, 701, 777
MaeIII GTNAC 2 cut(s) 167, 697
MalI GATC 8 cut(s) 441, 497, 564, 595, 600, 783, 791, 797
MbiI CCGCTC 2 cut(s) 540, 615
MboI GATC 8 cut(s) 439, 495, 562, 593, 598, 781, 789, 795
MboII GAAGA 5 cut(s) 15, 376, 526, 532, 776
MflI RGATCY 3 cut(s) 562, 598, 795
MluCI AATT 1 cut(s) 190
Mly113I GGCGCC 2 cut(s) 48, 264
MlyI GAGTC 2 cut(s) 21, 584
MmeI TCCRAC 1 cut(s) 786
MseI TTAA 1 cut(s) 814
MslI CAYNNNNRTG 1 cut(s) 647
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 3 cut(s) 90, 178, 500
MspR9I CCNGG 1 cut(s) 500
MvnI CGCG 1 cut(s) 269
NarI GGCGCC 2 cut(s) 48, 264
NciI CCSGG 1 cut(s) 500
NdeII GATC 8 cut(s) 439, 495, 562, 593, 598, 781, 789, 795
NlaIV GGNNCC 3 cut(s) 49, 94, 265
NmuCI GTSAC 1 cut(s) 167
OliI CACNNNNGTG 1 cut(s) 647
PcsI WCGNNNNNNNCGW 2 cut(s) 500, 515
PfeI GAWTC 1 cut(s) 711
PflFI GACNNNGTC 2 cut(s) 11, 742
PleI GAGTC 2 cut(s) 20, 583
PluTI GGCGCC 2 cut(s) 51, 267
PpsI GAGTC 2 cut(s) 20, 583
Ppu21I YACGTR 1 cut(s) 702
Psp1406I AACGTT 1 cut(s) 777
PspN4I GGNNCC 3 cut(s) 49, 94, 265
PspPI GGNCC 2 cut(s) 216, 807
PstI CTGCAG 1 cut(s) 636
PsuI RGATCY 3 cut(s) 562, 598, 795
PsyI GACNNNGTC 2 cut(s) 11, 742
RsaI GTAC 5 cut(s) 94, 115, 158, 546, 726
RsaNI GTAC 5 cut(s) 93, 114, 157, 545, 725
RseI CAYNNNNRTG 1 cut(s) 647
SacII CCGCGG 1 cut(s) 270
SaqAI TTAA 1 cut(s) 814
Sau3AI GATC 8 cut(s) 439, 495, 562, 593, 598, 781, 789, 795
Sau96I GGNCC 2 cut(s) 216, 807
SchI GAGTC 2 cut(s) 21, 584
ScrFI CCNGG 1 cut(s) 500
SfcI CTRYAG 1 cut(s) 632
SfoI GGCGCC 2 cut(s) 49, 265
Sfr303I CCGCGG 1 cut(s) 270
SgrBI CCGCGG 1 cut(s) 270
SinI GGWCC 2 cut(s) 216, 807
SmiMI CAYNNNNRTG 1 cut(s) 647
Sse9I AATT 1 cut(s) 190
SspDI GGCGCC 2 cut(s) 47, 263
StyD4I CCNGG 1 cut(s) 498
TaaI ACNGT 3 cut(s) 167, 549, 744
TaiI ACGT 6 cut(s) 317, 477, 557, 573, 704, 780
TaqI TCGA 5 cut(s) 362, 494, 567, 573, 659
TaqII GACCGA 1 cut(s) 24
TasI AATT 1 cut(s) 190
TauI GCSGC 9 cut(s) 48, 53, 56, 68, 77, 80, 189, 254, 269
TfiI GAWTC 1 cut(s) 711
Tru1I TTAA 1 cut(s) 814
Tru9I TTAA 1 cut(s) 814
TseFI GTSAC 1 cut(s) 167
TseI GCWGC 5 cut(s) 56, 183, 204, 207, 631
Tsp45I GTSAC 1 cut(s) 167
TspGWI ACGGA 3 cut(s) 307, 532, 745
Tth111I GACNNNGTC 2 cut(s) 11, 742
VpaK11BI GGWCC 2 cut(s) 216, 807
XmiI GTMKAC 2 cut(s) 15, 273
ZraI GACGTC 2 cut(s) 475, 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.