FvH4_2g32280

UPF0587 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005449
Physical Location & Seq
Reverse (-)
24512659 .. 24514842
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32280.t1

Sequence Viewer

Length: 504 bp
ATGGTGCTCCTGGCGCTACAGATAACGGCGGAGCTCGAAAACCTGACTGACCTTCAGCCCGAAAACGGCTGCGACGATCCCGACTTCACCTATCTCTTCAAGTTGCGATGCCAGAGCTGTGGTGAGTTGACTCAGAAGGAAACTGCTTTGAGCTTGAGCGAGTCCGTTCCTCTTCCGGTGGGCAAGGGCAACACTAATCTCATTCAGAAGTGCAAGTTTTGTGGCAGGGAAGGAACCATAACGATGGTGCCTGGTCGTGGTCGACCATTAACTCAGGAGTTAAGTGAAGCAGGGAAGTCTACCCCCGTGATGGCGTTCGAATGCAGGGGTTATGAGCCGGTGGATTACGTTTTTGGTGGCGGGTGGAAGGCTCGATCATTGGCTGGGACAACCTTTGACAATGTTGACCTGTCAGAGGATGAATTTGCTGAATATGATGAGAAAGGAGAGTGCCCAGTTATGATCTCCAAGCACTCTTCTTCTTTTGTTGTGCTAAAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF866

Protein Analysis

168

Amino Acids

18.33

Weight (kDa)

4.65

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CXXC_Zn-b_euk PF05907 5 - 162 1.4e-50 CXXC motif containing zinc binding protein, eukaryotic
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 247
AccI GTMKAC 2 cut(s) 262, 299
AciI CCGC 2 cut(s) 29, 360
AclWI GGATC 1 cut(s) 71
AcsI RAATTY 1 cut(s) 422
AcuI CTGAAG 1 cut(s) 38
AfiI CCNNNNNNNGG 4 cut(s) 65, 257, 310, 415
AgsI TTSAA 1 cut(s) 100
AjnI CCWGG 2 cut(s) 9, 250
AluBI AGCT 3 cut(s) 34, 117, 153
AluI AGCT 3 cut(s) 34, 117, 153
Alw21I GWGCWC 2 cut(s) 9, 36
AlwI GGATC 1 cut(s) 71
ApeKI GCWGC 1 cut(s) 69
ApoI RAATTY 1 cut(s) 422
AspLEI GCGC 1 cut(s) 16
AsuHPI GGTGA 2 cut(s) 79, 134
AsuII TTCGAA 1 cut(s) 318
BaeGI GKGCMC 1 cut(s) 455
BanI GGYRCC 1 cut(s) 247
BanII GRGCYC 1 cut(s) 36
Bbv12I GWGCWC 2 cut(s) 9, 36
BbvI GCAGC 1 cut(s) 56
BccI CCATC 2 cut(s) 238, 304
BceAI ACGGC 2 cut(s) 42, 82
BciT130I CCWGG 2 cut(s) 11, 252
BfaI CTAG 1 cut(s) 502
BfmI CTRYAG 1 cut(s) 17
BfoI RGCGCY 1 cut(s) 17
BisI GCNGC 1 cut(s) 70
BlsI GCNGC 1 cut(s) 71
Bme1390I CCNGG 2 cut(s) 11, 252
BmiI GGNNCC 2 cut(s) 235, 249
BmrFI CCNGG 2 cut(s) 11, 252
BmrI ACTGGG 1 cut(s) 449
BmsI GCATC 1 cut(s) 98
BmuI ACTGGG 1 cut(s) 449
Bpu14I TTCGAA 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 175
BsaWI WCCGGW 1 cut(s) 175
Bsc4I CCNNNNNNNGG 4 cut(s) 65, 257, 310, 415
Bse118I RCCGGY 1 cut(s) 337
Bse1I ACTGG 1 cut(s) 455
BseBI CCWGG 2 cut(s) 11, 252
BseGI GGATG 1 cut(s) 424
BseLI CCNNNNNNNGG 4 cut(s) 65, 257, 310, 415
BseMII CTCAG 2 cut(s) 146, 287
BseNI ACTGG 1 cut(s) 455
BseSI GKGCMC 1 cut(s) 455
BseXI GCAGC 1 cut(s) 56
BseYI CCCAGC 1 cut(s) 383
BshNI GGYRCC 1 cut(s) 247
BsiHKAI GWGCWC 2 cut(s) 9, 36
BsiSI CCGG 2 cut(s) 176, 338
BslFI GGGAC 1 cut(s) 400
BslI CCNNNNNNNGG 4 cut(s) 65, 257, 310, 415
BsmFI GGGAC 1 cut(s) 400
BsmI GAATGC 1 cut(s) 326
Bsp119I TTCGAA 1 cut(s) 318
Bsp1286I GDGCHC 3 cut(s) 9, 36, 455
Bsp143I GATC 3 cut(s) 76, 374, 462
BspACI CCGC 2 cut(s) 29, 360
BspCNI CTCAG 2 cut(s) 145, 286
BspLI GGNNCC 2 cut(s) 235, 249
BspPI GGATC 1 cut(s) 71
BspT104I TTCGAA 1 cut(s) 318
BspT107I GGYRCC 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 337
BsrI ACTGG 1 cut(s) 455
BssAI RCCGGY 1 cut(s) 337
BssMI GATC 3 cut(s) 76, 374, 462
Bst2UI CCWGG 2 cut(s) 11, 252
Bst6I CTCTTC 3 cut(s) 101, 177, 481
BstBI TTCGAA 1 cut(s) 318
BstDEI CTNAG 2 cut(s) 132, 273
BstENI CCTNNNNNAGG 1 cut(s) 413
BstF5I GGATG 1 cut(s) 424
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 3 cut(s) 79, 377, 465
BstMBI GATC 3 cut(s) 76, 374, 462
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstNI CCWGG 2 cut(s) 11, 252
BstSCI CCNGG 2 cut(s) 9, 250
BstSFI CTRYAG 1 cut(s) 17
BstSLI GKGCMC 1 cut(s) 455
BstV1I GCAGC 1 cut(s) 56
BstXI CCANNNNNNTGG 2 cut(s) 119, 244
BtgZI GCGATG 1 cut(s) 121
BtsCI GGATG 1 cut(s) 424
CfoI GCGC 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 337
CspCI CAANNNNNGTGG 2 cut(s) 202, 237
CviJI RGCY 8 cut(s) 34, 58, 69, 117, 153, 337, 371, 383
CviKI_1 RGCY 8 cut(s) 34, 58, 69, 117, 153, 337, 371, 383
DdeI CTNAG 2 cut(s) 132, 273
DpnI GATC 3 cut(s) 78, 376, 464
DpnII GATC 3 cut(s) 76, 374, 462
Eam1104I CTCTTC 3 cut(s) 101, 177, 481
EarI CTCTTC 3 cut(s) 101, 177, 481
EciI GGCGGA 1 cut(s) 44
Ecl136II GAGCTC 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 36
Eco53kI GAGCTC 1 cut(s) 34
Eco57I CTGAAG 1 cut(s) 38
EcoICRI GAGCTC 1 cut(s) 34
EcoNI CCTNNNNNAGG 1 cut(s) 413
EcoRII CCWGG 2 cut(s) 9, 250
EcoT38I GRGCYC 1 cut(s) 36
FaiI YATR 4 cut(s) 239, 333, 435, 461
FaqI GGGAC 1 cut(s) 400
FauI CCCGC 1 cut(s) 353
FblI GTMKAC 2 cut(s) 262, 299
Fnu4HI GCNGC 1 cut(s) 70
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 1 cut(s) 70
FspBI CTAG 1 cut(s) 502
GlaI GCGC 1 cut(s) 15
GluI GCNGC 1 cut(s) 70
GsaI CCCAGC 1 cut(s) 387
HaeII RGCGCY 1 cut(s) 17
HapII CCGG 2 cut(s) 176, 338
HhaI GCGC 1 cut(s) 16
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HincII GTYRAC 3 cut(s) 129, 263, 406
HindII GTYRAC 3 cut(s) 129, 263, 406
HinfI GANTC 2 cut(s) 130, 161
HpaII CCGG 2 cut(s) 176, 338
HphI GGTGA 2 cut(s) 79, 134
Hpy166II GTNNAC 4 cut(s) 129, 263, 300, 406
Hpy188I TCNGA 3 cut(s) 135, 207, 415
Hpy188III TCNNGA 2 cut(s) 80, 275
Hpy8I GTNNAC 4 cut(s) 129, 263, 300, 406
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 4 cut(s) 62, 130, 224, 361
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 2 cut(s) 213, 324
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
HpyF3I CTNAG 2 cut(s) 132, 273
HpySE526I ACGT 1 cut(s) 348
HspAI GCGC 1 cut(s) 14
Kzo9I GATC 3 cut(s) 76, 374, 462
LmnI GCTCC 2 cut(s) 12, 31
Lsp1109I GCAGC 1 cut(s) 56
LweI GCATC 1 cut(s) 98
MaeI CTAG 1 cut(s) 502
MaeII ACGT 1 cut(s) 348
MalI GATC 3 cut(s) 78, 376, 464
MboI GATC 3 cut(s) 76, 374, 462
MboII GAAGA 4 cut(s) 88, 164, 468, 471
MhlI GDGCHC 3 cut(s) 9, 36, 455
MluCI AATT 1 cut(s) 422
MlyI GAGTC 2 cut(s) 124, 170
MnlI CCTC 2 cut(s) 180, 409
MseI TTAA 2 cut(s) 269, 281
MslI CAYNNNNRTG 1 cut(s) 242
MspI CCGG 2 cut(s) 176, 338
MspR9I CCNGG 2 cut(s) 11, 252
Mva1269I GAATGC 1 cut(s) 326
MvaI CCWGG 2 cut(s) 11, 252
MwoI GCNNNNNNNGC 1 cut(s) 13
NdeII GATC 3 cut(s) 76, 374, 462
NlaIV GGNNCC 2 cut(s) 235, 249
NspV TTCGAA 1 cut(s) 318
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PctI GAATGC 1 cut(s) 326
PkrI GCNGC 1 cut(s) 71
PleI GAGTC 2 cut(s) 124, 169
PpsI GAGTC 2 cut(s) 124, 169
Psp124BI GAGCTC 1 cut(s) 36
Psp6I CCWGG 2 cut(s) 9, 250
PspFI CCCAGC 1 cut(s) 383
PspGI CCWGG 2 cut(s) 9, 250
PspN4I GGNNCC 2 cut(s) 235, 249
RseI CAYNNNNRTG 1 cut(s) 242
SacI GAGCTC 1 cut(s) 36
SalI GTCGAC 1 cut(s) 261
SaqAI TTAA 2 cut(s) 269, 281
SatI GCNGC 1 cut(s) 70
Sau3AI GATC 3 cut(s) 76, 374, 462
SchI GAGTC 2 cut(s) 124, 170
ScrFI CCNGG 2 cut(s) 11, 252
SduI GDGCHC 3 cut(s) 9, 36, 455
SetI ASST 9 cut(s) 36, 45, 54, 92, 119, 155, 351, 395, 411
SfaNI GCATC 1 cut(s) 98
SfcI CTRYAG 1 cut(s) 17
SfuI TTCGAA 1 cut(s) 318
SmiMI CAYNNNNRTG 1 cut(s) 242
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
Sse9I AATT 1 cut(s) 422
SsiI CCGC 2 cut(s) 29, 360
SspMI CTAG 1 cut(s) 502
SstI GAGCTC 1 cut(s) 36
StyD4I CCNGG 2 cut(s) 9, 250
TaiI ACGT 1 cut(s) 351
TaqI TCGA 4 cut(s) 36, 262, 318, 373
TasI AATT 1 cut(s) 422
Tru1I TTAA 2 cut(s) 269, 281
Tru9I TTAA 2 cut(s) 269, 281
TseI GCWGC 1 cut(s) 69
TspDTI ATGAA 1 cut(s) 435
TspGWI ACGGA 1 cut(s) 154
XagI CCTNNNNNAGG 1 cut(s) 413
XapI RAATTY 1 cut(s) 422
XmiI GTMKAC 2 cut(s) 262, 299
XspI CTAG 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.