MD08G1073900.v1.1

UPF0587 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005449
Physical Location & Seq
Reverse (-)
5966119 .. 5967753
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1073900.v1.1.491

Sequence Viewer

Length: 555 bp
ATGGTGAAGTTCTTGCTTCAGATCTCCGCCGAGCTCAAGAATCTGACCGACCTTCAGCCGCAGGACGGCTGCGACGACCCGGACTACTCCTATTTCTTCAAGCTGAGATGTGAGTGGTGTGGAGAGCTGAGCCCGAGAGAAACTTTTGTGAGCTTGAACGAGACTGTTCCTCTTCCAGCCCGCAATAAGAGAACCGCTAATGTCATCCGCAAGAGAACCGCTAAGGTCATCCGCAGGAGAACCGCTAATGTCATCCAGAAGTGCAAGCTTTGTGAGCGGGTTGGAATGATCCTGATGCACCCAGGCCTTGGCATGCCACTGACCCAGGCAGCAAGTGATGCCAGGCAGTTCGTCCCCGTGATGATTTTTGATTTCAGAGGTTTTGTACCTGTGGACTCTGTATTTGTTGGTGGCTGGAGAGCTGAGTCTCTGAGTGGCATAAATTTTGAGCACATTGACTTGTCGGCAAGGAAGTTTGTTGAGTATGATGTGGAGTACCCAGTTATGATTTCTAATCTTCAGGCTGCTGGTGGTTGGGTCTTTTATGCCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF866

Protein Analysis

185

Amino Acids

20.93

Weight (kDa)

8.62

Isoelectric Point (pI)

53.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CXXC_Zn-b_euk PF05907 6 - 175 1.3e-29 CXXC motif containing zinc binding protein, eukaryotic
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 308
AccBSI CCGCTC 1 cut(s) 277
AciI CCGC 9 cut(s) 27, 59, 181, 195, 208, 219, 232, 243, 277
AclWI GGATC 1 cut(s) 283
AcsI RAATTY 1 cut(s) 442
AcuI CTGAAG 2 cut(s) 38, 503
AfaI GTAC 2 cut(s) 387, 497
AfiI CCNNNNNNNGG 2 cut(s) 65, 308
AgsI TTSAA 2 cut(s) 100, 157
AjnI CCWGG 3 cut(s) 301, 324, 341
AluBI AGCT 6 cut(s) 34, 103, 127, 153, 268, 422
AluI AGCT 6 cut(s) 34, 103, 127, 153, 268, 422
Alw21I GWGCWC 2 cut(s) 36, 453
Alw26I GTCTC 2 cut(s) 155, 432
AlwI GGATC 1 cut(s) 283
Ama87I CYCGRG 1 cut(s) 133
AoxI GGCC 1 cut(s) 304
ApeKI GCWGC 3 cut(s) 69, 329, 524
ApoI RAATTY 1 cut(s) 442
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 133
BanII GRGCYC 2 cut(s) 36, 134
Bbv12I GWGCWC 2 cut(s) 36, 453
BbvI GCAGC 3 cut(s) 56, 341, 511
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 3 cut(s) 303, 326, 343
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 2 cut(s) 155, 432
BglII AGATCT 1 cut(s) 21
BisI GCNGC 4 cut(s) 59, 70, 330, 525
BlpI GCTNAGC 1 cut(s) 128
BlsI GCNGC 4 cut(s) 60, 71, 331, 526
Bme1390I CCNGG 4 cut(s) 80, 303, 326, 343
BmeT110I CYCGRG 1 cut(s) 133
BmrFI CCNGG 4 cut(s) 80, 303, 326, 343
BmrI ACTGGG 1 cut(s) 494
BmsI GCATC 2 cut(s) 285, 328
BmuI ACTGGG 1 cut(s) 494
BpmI CTGGAG 1 cut(s) 436
Bpu10I CCTNAGC 1 cut(s) 222
Bpu1102I GCTNAGC 1 cut(s) 128
BpuEI CTTGAG 1 cut(s) 20
BpuMI CCSGG 1 cut(s) 80
BsaJI CCNNGG 3 cut(s) 301, 307, 324
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 308
Bse1I ACTGG 1 cut(s) 500
BseBI CCWGG 3 cut(s) 303, 326, 343
BseDI CCNNGG 3 cut(s) 301, 307, 324
BseGI GGATG 3 cut(s) 204, 228, 252
BseLI CCNNNNNNNGG 2 cut(s) 65, 308
BseMII CTCAG 4 cut(s) 95, 119, 414, 422
BseNI ACTGG 1 cut(s) 500
BseXI GCAGC 3 cut(s) 56, 341, 511
BshFI GGCC 1 cut(s) 306
BsiHKAI GWGCWC 2 cut(s) 36, 453
BsiHKCI CYCGRG 1 cut(s) 133
BsiSI CCGG 1 cut(s) 80
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 2 cut(s) 65, 308
BsmAI GTCTC 2 cut(s) 155, 432
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 1 cut(s) 306
BsoBI CYCGRG 1 cut(s) 133
Bsp1286I GDGCHC 3 cut(s) 36, 134, 453
Bsp143I GATC 2 cut(s) 21, 288
Bsp1720I GCTNAGC 1 cut(s) 128
BspACI CCGC 9 cut(s) 27, 59, 181, 195, 208, 219, 232, 243, 277
BspANI GGCC 1 cut(s) 306
BspCNI CTCAG 4 cut(s) 96, 120, 415, 423
BspPI GGATC 1 cut(s) 283
BsrBI CCGCTC 1 cut(s) 277
BsrI ACTGG 1 cut(s) 500
BssECI CCNNGG 3 cut(s) 301, 307, 324
BssMI GATC 2 cut(s) 21, 288
BssT1I CCWWGG 1 cut(s) 307
Bst2UI CCWGG 3 cut(s) 303, 326, 343
Bst4CI ACNGT 1 cut(s) 166
Bst6I CTCTTC 1 cut(s) 177
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 3 cut(s) 181, 266, 314
BstDEI CTNAG 5 cut(s) 104, 128, 222, 423, 431
BstF5I GGATG 3 cut(s) 204, 228, 252
BstKTI GATC 2 cut(s) 24, 291
BstMAI GTCTC 2 cut(s) 155, 432
BstMBI GATC 2 cut(s) 21, 288
BstMWI GCNNNNNNNGC 2 cut(s) 274, 338
BstNI CCWGG 3 cut(s) 303, 326, 343
BstNSI RCATGY 1 cut(s) 316
BstSCI CCNGG 4 cut(s) 78, 301, 324, 341
BstV1I GCAGC 3 cut(s) 56, 341, 511
BstX2I RGATCY 1 cut(s) 21
BstYI RGATCY 1 cut(s) 21
BsuRI GGCC 1 cut(s) 306
BtsCI GGATG 3 cut(s) 204, 228, 252
BtsIMutI CAGTG 1 cut(s) 317
Cac8I GCNNGC 3 cut(s) 181, 266, 314
Csp6I GTAC 2 cut(s) 386, 496
CviAII CATG 1 cut(s) 313
CviQI GTAC 2 cut(s) 386, 496
DdeI CTNAG 5 cut(s) 104, 128, 222, 423, 431
DpnI GATC 2 cut(s) 23, 290
DpnII GATC 2 cut(s) 21, 288
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
EciI GGCGGA 1 cut(s) 16
Ecl136II GAGCTC 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 307
Eco147I AGGCCT 1 cut(s) 306
Eco24I GRGCYC 2 cut(s) 36, 134
Eco53kI GAGCTC 1 cut(s) 34
Eco57I CTGAAG 2 cut(s) 38, 503
Eco88I CYCGRG 1 cut(s) 133
EcoICRI GAGCTC 1 cut(s) 34
EcoRII CCWGG 3 cut(s) 301, 324, 341
EcoT14I CCWWGG 1 cut(s) 307
EcoT38I GRGCYC 2 cut(s) 36, 134
ErhI CCWWGG 1 cut(s) 307
FaeI CATG 1 cut(s) 316
FaiI YATR 5 cut(s) 314, 440, 486, 506, 546
FaqI GGGAC 1 cut(s) 338
FatI CATG 1 cut(s) 312
FauI CCCGC 2 cut(s) 188, 270
Fnu4HI GCNGC 4 cut(s) 59, 70, 330, 525
FokI GGATG 3 cut(s) 191, 215, 239
FriOI GRGCYC 2 cut(s) 36, 134
Fsp4HI GCNGC 4 cut(s) 59, 70, 330, 525
GluI GCNGC 4 cut(s) 59, 70, 330, 525
GsuI CTGGAG 1 cut(s) 436
HaeIII GGCC 1 cut(s) 306
HapII CCGG 1 cut(s) 80
Hin1II CATG 1 cut(s) 316
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 3 cut(s) 40, 395, 425
HpaII CCGG 1 cut(s) 80
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 394
Hpy188I TCNGA 4 cut(s) 21, 45, 377, 432
Hpy188III TCNNGA 3 cut(s) 37, 256, 292
Hpy8I GTNNAC 1 cut(s) 394
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4V TGCA 2 cut(s) 264, 298
HpyF10VI GCNNNNNNNGC 2 cut(s) 274, 338
HpyF3I CTNAG 5 cut(s) 104, 128, 222, 423, 431
Hsp92II CATG 1 cut(s) 316
Kzo9I GATC 2 cut(s) 21, 288
Lsp1109I GCAGC 3 cut(s) 56, 341, 511
LweI GCATC 2 cut(s) 285, 328
MalI GATC 2 cut(s) 23, 290
MbiI CCGCTC 1 cut(s) 277
MboI GATC 2 cut(s) 21, 288
MboII GAAGA 3 cut(s) 88, 164, 509
MflI RGATCY 1 cut(s) 21
MhlI GDGCHC 3 cut(s) 36, 134, 453
MluCI AATT 1 cut(s) 442
MlyI GAGTC 2 cut(s) 389, 434
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 2 cut(s) 180, 371
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 4 cut(s) 80, 303, 326, 343
MvaI CCWGG 3 cut(s) 303, 326, 343
MwoI GCNNNNNNNGC 2 cut(s) 274, 338
NciI CCSGG 1 cut(s) 80
NdeII GATC 2 cut(s) 21, 288
NlaIII CATG 1 cut(s) 316
NmeAIII GCCGAG 1 cut(s) 55
NspI RCATGY 1 cut(s) 316
PaeI GCATGC 1 cut(s) 316
PceI AGGCCT 1 cut(s) 306
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PfeI GAWTC 1 cut(s) 40
PflMI CCANNNNNTGG 1 cut(s) 308
PkrI GCNGC 4 cut(s) 60, 71, 331, 526
PleI GAGTC 2 cut(s) 389, 433
PpsI GAGTC 2 cut(s) 389, 433
Psp124BI GAGCTC 1 cut(s) 36
Psp6I CCWGG 3 cut(s) 301, 324, 341
PspGI CCWGG 3 cut(s) 301, 324, 341
PsuI RGATCY 1 cut(s) 21
RsaI GTAC 2 cut(s) 387, 497
RsaNI GTAC 2 cut(s) 386, 496
SacI GAGCTC 1 cut(s) 36
SatI GCNGC 4 cut(s) 59, 70, 330, 525
Sau3AI GATC 2 cut(s) 21, 288
SchI GAGTC 2 cut(s) 389, 434
ScrFI CCNGG 4 cut(s) 80, 303, 326, 343
SduI GDGCHC 3 cut(s) 36, 134, 453
SfaNI GCATC 2 cut(s) 285, 328
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
SphI GCATGC 1 cut(s) 316
Sse9I AATT 1 cut(s) 442
SseBI AGGCCT 1 cut(s) 306
SsiI CCGC 9 cut(s) 27, 59, 181, 195, 208, 219, 232, 243, 277
SstI GAGCTC 1 cut(s) 36
StuI AGGCCT 1 cut(s) 306
StyD4I CCNGG 4 cut(s) 78, 301, 324, 341
StyI CCWWGG 1 cut(s) 307
TaaI ACNGT 1 cut(s) 166
TaqII GACCGA 1 cut(s) 62
TasI AATT 1 cut(s) 442
TauI GCSGC 1 cut(s) 61
TfiI GAWTC 1 cut(s) 40
TscAI CASTG 1 cut(s) 324
TseI GCWGC 3 cut(s) 69, 329, 524
TspRI CASTG 1 cut(s) 324
Van91I CCANNNNNTGG 1 cut(s) 308
XapI RAATTY 1 cut(s) 442
XceI RCATGY 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.