FvH4_2g32730

protein KIAA0930 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004749
Physical Location & Seq
Reverse (-)
24739875 .. 24743633
3759 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32730.t2

Sequence Viewer

Length: 1161 bp
ATGCTCGGAGACGGTGAAGAAACCCCCTCCAGATGCGAGCTCCTCACCATGGTGAAGAAGCACTCCACCTTGCTGGGGAAAACAGTCGTCGACGCCTCCGATGTCGAAATGGACCCCCGCTTCTGGCACGACGTCTTCGATTTCTACTTCCTCCGCGGCAAAGACTCCCGCCGCCGCCAGGACGACGATCTCGTCTTCTTCGTCAGGAAATTGAGCTCACAGGGCTTCAACGACGACGTTCCGCCTTACTTTGTGCGCAGGTGGGCTCCTAAGTTGGATAATTTGTTAGGTGAAAAAGCTTTAGAGGTGGATTGGAGGCGCTCCTTTCACTTGAATTTGATTGCTCACACTACATTTACTGTCACAGTCGCTATTTGCAGTCATCAGGACCTTCGGAATCATCAAGCTGAGCAAGATAAACCATTATCTCCAATATACAAGGTAGTAAAGACTGTTTATGCATCTCCCAGCCGTGTCAACTTTCATTTGGACTCGCAAAAGGAAGTGGAGACAACACCTGCTTATCCAGATATATGTTTTGCAGTTGATGACTTTGACTCCACTTTTGATGCCGTGGTATTGACAGAAACAGACCATTGCTATTGTGTAATACTCAATGCACATGATGGGGCAGCATTTCCCACGGCAAAGGAGCCTGATGATTGTAGTTCTAGTGATACTTCCTCTTTGAAGGTTGACATTAGTTCTGTAAAGGCCAAAAGTTCCAAGCTAACTCTTTTTTCGGGATTTGTCAGCTATCAAATGGTACGAGATGCATATGATGCCGGCAAGTCTCGATTTGGGAACCTTTTGTCACTTGCTCATTCCCCTGGCAAACAAGATAGACTGTACATGAAAGGACCTGGAGGCCATGGGGAAGTTGAGGTGGCTGTTTCTGGTGTTGTAGATCAGAGCCAGCAAGACACAGGTCCTTTTTCACCTGTTACATCCAAGACAGGATTCGGTATTGGCTTTATGGTTCGTAAAGCAGCTTCTGTTGCATCTGTTGCGGCAAAGAATGCCTATGCGGCTGCTGCTTCTAGTCATGGTTTTGATGAGGAGTTGATCCCTCTCAAATGCTGCTTGATGTCCATTTCATTACCGTGGGAACACATTGCATATGATCTTCTTTTTAAGGGGACTCCTCCAGTGAACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2045

Protein Analysis

387

Amino Acids

42.74

Weight (kDa)

5.71

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2045 PF09741 40 - 301 1.3e-77 Uncharacterized conserved protein (DUF2045)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 249, 526
AasI GACNNNNNNGTC 1 cut(s) 191
AatII GACGTC 1 cut(s) 135
Acc16I TGCGCA 1 cut(s) 257
Acc36I ACCTGC 2 cut(s) 249, 526
AccI GTMKAC 1 cut(s) 90
AccII CGCG 1 cut(s) 156
AciI CCGC 9 cut(s) 118, 154, 156, 169, 172, 175, 242, 1010, 1028
AclWI GGATC 1 cut(s) 1060
AcsI RAATTY 1 cut(s) 334
AcyI GRCGYC 2 cut(s) 93, 132
AfaI GTAC 2 cut(s) 768, 851
AfiI CCNNNNNNNGG 4 cut(s) 49, 75, 123, 178
AgsI TTSAA 3 cut(s) 229, 334, 691
AjnI CCWGG 3 cut(s) 177, 829, 862
AjuI GAANNNNNNNTTGG 2 cut(s) 944, 976
AleI CACNNNNGTG 1 cut(s) 50
AluBI AGCT 7 cut(s) 40, 216, 299, 407, 730, 756, 992
AluI AGCT 7 cut(s) 40, 216, 299, 407, 730, 756, 992
Alw21I GWGCWC 2 cut(s) 42, 218
Alw26I GTCTC 3 cut(s) 3, 503, 798
AlwI GGATC 1 cut(s) 1060
AlwNI CAGNNNCTG 1 cut(s) 995
AoxI GGCC 2 cut(s) 714, 868
ApeKI GCWGC 5 cut(s) 632, 989, 1031, 1034, 1080
ApoI RAATTY 1 cut(s) 334
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
AspLEI GCGC 2 cut(s) 258, 321
AspS9I GGNCC 4 cut(s) 112, 388, 860, 929
AsuHPI GGTGA 5 cut(s) 26, 37, 64, 302, 930
AvaII GGWCC 4 cut(s) 112, 388, 860, 929
BanII GRGCYC 3 cut(s) 42, 218, 268
BarI GAAGNNNNNNTAC 2 cut(s) 976, 1008
BbsI GAAGAC 2 cut(s) 127, 187
Bbv12I GWGCWC 2 cut(s) 42, 218
BbvI GCAGC 5 cut(s) 644, 1001, 1018, 1021, 1067
BccI CCATC 1 cut(s) 620
BceAI ACGGC 3 cut(s) 456, 557, 660
BciT130I CCWGG 3 cut(s) 179, 831, 864
BcoDI GTCTC 3 cut(s) 3, 503, 798
BfaI CTAG 2 cut(s) 672, 1041
BfoI RGCGCY 1 cut(s) 322
BfuAI ACCTGC 2 cut(s) 249, 526
BglI GCCNNNNNGGC 1 cut(s) 1028
BlpI GCTNAGC 1 cut(s) 408
Bme1390I CCNGG 3 cut(s) 179, 831, 864
Bme18I GGWCC 4 cut(s) 112, 388, 860, 929
BmgT120I GGNCC 4 cut(s) 112, 388, 860, 929
BmiI GGNNCC 4 cut(s) 114, 267, 654, 806
BmrFI CCNGG 3 cut(s) 179, 831, 864
BmsI GCATC 6 cut(s) 23, 470, 559, 763, 772, 1010
BoxI GACNNNNGTC 1 cut(s) 927
BpiI GAAGAC 2 cut(s) 127, 187
BpmI CTGGAG 3 cut(s) 13, 885, 1131
Bpu1102I GCTNAGC 1 cut(s) 408
BsaHI GRCGYC 2 cut(s) 93, 132
BsaJI CCNNGG 7 cut(s) 48, 154, 573, 642, 829, 871, 1103
BsaXI ACNNNNNCTCC 2 cut(s) 542, 572
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 75, 123, 178
Bse118I RCCGGY 1 cut(s) 785
Bse1I ACTGG 1 cut(s) 1148
Bse3DI GCAATG 2 cut(s) 595, 1113
BseBI CCWGG 3 cut(s) 179, 831, 864
BseDI CCNNGG 7 cut(s) 48, 154, 573, 642, 829, 871, 1103
BseGI GGATG 1 cut(s) 947
BseLI CCNNNNNNNGG 4 cut(s) 49, 75, 123, 178
BseMI GCAATG 2 cut(s) 595, 1113
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 1 cut(s) 1148
BseRI GAGGAG 3 cut(s) 32, 1073, 1134
BseXI GCAGC 5 cut(s) 644, 1001, 1018, 1021, 1067
BseYI CCCAGC 2 cut(s) 73, 467
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 2 cut(s) 716, 870
BsiHKAI GWGCWC 2 cut(s) 42, 218
BsiSI CCGG 1 cut(s) 786
BslFI GGGAC 1 cut(s) 1153
BslI CCNNNNNNNGG 4 cut(s) 49, 75, 123, 178
BsmAI GTCTC 3 cut(s) 3, 503, 798
BsmBI CGTCTC 1 cut(s) 3
BsmFI GGGAC 1 cut(s) 1153
BsmI GAATGC 1 cut(s) 1024
BsnI GGCC 2 cut(s) 716, 870
Bsp1286I GDGCHC 3 cut(s) 42, 218, 268
Bsp1407I TGTACA 1 cut(s) 849
Bsp143I GATC 4 cut(s) 187, 907, 1065, 1123
Bsp1720I GCTNAGC 1 cut(s) 408
Bsp19I CCATGG 2 cut(s) 48, 871
BspACI CCGC 9 cut(s) 118, 154, 156, 169, 172, 175, 242, 1010, 1028
BspANI GGCC 2 cut(s) 716, 870
BspCNI CTCAG 1 cut(s) 400
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 4 cut(s) 114, 267, 654, 806
BspMI ACCTGC 2 cut(s) 249, 526
BspPI GGATC 1 cut(s) 1060
BsrDI GCAATG 2 cut(s) 595, 1113
BsrFI RCCGGY 1 cut(s) 785
BsrGI TGTACA 1 cut(s) 849
BsrI ACTGG 1 cut(s) 1148
BssAI RCCGGY 1 cut(s) 785
BssECI CCNNGG 7 cut(s) 48, 154, 573, 642, 829, 871, 1103
BssMI GATC 4 cut(s) 187, 907, 1065, 1123
BssNI GRCGYC 2 cut(s) 93, 132
BssT1I CCWWGG 2 cut(s) 48, 871
Bst2UI CCWGG 3 cut(s) 179, 831, 864
Bst4CI ACNGT 7 cut(s) 14, 85, 361, 367, 454, 849, 1104
BstACI GRCGYC 2 cut(s) 93, 132
BstAPI GCANNNNNTGC 3 cut(s) 782, 1007, 1019
BstAUI TGTACA 1 cut(s) 849
BstC8I GCNNGC 3 cut(s) 38, 787, 917
BstDEI CTNAG 2 cut(s) 270, 408
BstDSI CCRYGG 6 cut(s) 48, 154, 573, 642, 871, 1103
BstF5I GGATG 1 cut(s) 947
BstFNI CGCG 1 cut(s) 156
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 2 cut(s) 258, 321
BstKTI GATC 4 cut(s) 190, 910, 1068, 1126
BstMAI GTCTC 3 cut(s) 3, 503, 798
BstMBI GATC 4 cut(s) 187, 907, 1065, 1123
BstMWI GCNNNNNNNGC 7 cut(s) 222, 782, 998, 1007, 1019, 1028, 1034
BstNI CCWGG 3 cut(s) 179, 831, 864
BstPAI GACNNNNGTC 1 cut(s) 927
BstSCI CCNGG 3 cut(s) 177, 829, 862
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 5 cut(s) 644, 1001, 1018, 1021, 1067
BstV2I GAAGAC 2 cut(s) 127, 187
BstXI CCANNNNNNTGG 1 cut(s) 73
BsuRI GGCC 2 cut(s) 716, 870
BtgI CCRYGG 6 cut(s) 48, 154, 573, 642, 871, 1103
BtsCI GGATG 1 cut(s) 947
BtsIMutI CAGTG 1 cut(s) 1155
BveI ACCTGC 2 cut(s) 249, 526
Cac8I GCNNGC 3 cut(s) 38, 787, 917
CaiI CAGNNNCTG 1 cut(s) 995
CfoI GCGC 2 cut(s) 258, 321
Cfr10I RCCGGY 1 cut(s) 785
Cfr13I GGNCC 4 cut(s) 112, 388, 860, 929
Cfr42I CCGCGG 1 cut(s) 157
CseI GACGC 1 cut(s) 101
Csp6I GTAC 2 cut(s) 767, 850
CviAII CATG 5 cut(s) 49, 623, 853, 872, 1046
CviQI GTAC 2 cut(s) 767, 850
DdeI CTNAG 2 cut(s) 270, 408
DpnI GATC 4 cut(s) 189, 909, 1067, 1125
DpnII GATC 4 cut(s) 187, 907, 1065, 1123
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
EciI GGCGGA 1 cut(s) 231
Ecl136II GAGCTC 2 cut(s) 40, 216
Eco130I CCWWGG 2 cut(s) 48, 871
Eco24I GRGCYC 3 cut(s) 42, 218, 268
Eco47I GGWCC 4 cut(s) 112, 388, 860, 929
Eco53kI GAGCTC 2 cut(s) 40, 216
EcoICRI GAGCTC 2 cut(s) 40, 216
EcoO109I RGGNCCY 3 cut(s) 388, 860, 929
EcoRII CCWGG 3 cut(s) 177, 829, 862
EcoT14I CCWWGG 2 cut(s) 48, 871
EcoT22I ATGCAT 2 cut(s) 463, 778
EcoT38I GRGCYC 3 cut(s) 42, 218, 268
ErhI CCWWGG 2 cut(s) 48, 871
Esp3I CGTCTC 1 cut(s) 3
FaeI CATG 5 cut(s) 52, 626, 856, 875, 1049
FaqI GGGAC 1 cut(s) 1153
FatI CATG 5 cut(s) 48, 622, 852, 871, 1045
FauI CCCGC 2 cut(s) 125, 176
FauNDI CATATG 2 cut(s) 778, 1120
FblI GTMKAC 1 cut(s) 90
FokI GGATG 1 cut(s) 934
FriOI GRGCYC 3 cut(s) 42, 218, 268
FspBI CTAG 2 cut(s) 672, 1041
FspI TGCGCA 1 cut(s) 257
GlaI GCGC 2 cut(s) 257, 320
GsaI CCCAGC 2 cut(s) 77, 471
GsuI CTGGAG 3 cut(s) 13, 885, 1131
HaeII RGCGCY 1 cut(s) 322
HaeIII GGCC 2 cut(s) 716, 870
HapII CCGG 1 cut(s) 786
HgaI GACGC 1 cut(s) 101
HhaI GCGC 2 cut(s) 258, 321
Hin1I GRCGYC 2 cut(s) 93, 132
Hin1II CATG 5 cut(s) 52, 626, 856, 875, 1049
Hin6I GCGC 2 cut(s) 256, 319
HinP1I GCGC 2 cut(s) 256, 319
HincII GTYRAC 3 cut(s) 91, 478, 697
HindII GTYRAC 3 cut(s) 91, 478, 697
HindIII AAGCTT 1 cut(s) 297
HinfI GANTC 6 cut(s) 164, 397, 491, 557, 960, 1141
HpaII CCGG 1 cut(s) 786
HphI GGTGA 5 cut(s) 26, 37, 64, 302, 930
Hpy166II GTNNAC 4 cut(s) 91, 478, 697, 1153
Hpy188I TCNGA 4 cut(s) 8, 100, 396, 912
Hpy188III TCNNGA 6 cut(s) 30, 205, 386, 527, 744, 795
Hpy8I GTNNAC 4 cut(s) 91, 478, 697, 1153
Hpy99I CGWCG 6 cut(s) 92, 95, 134, 188, 236, 239
HpyAV CCTTC 2 cut(s) 401, 685
HpyCH4III ACNGT 7 cut(s) 14, 85, 361, 367, 454, 849, 1104
HpyCH4IV ACGT 2 cut(s) 132, 237
HpyCH4V TGCA 7 cut(s) 378, 461, 542, 620, 776, 1001, 1118
HpyF10VI GCNNNNNNNGC 7 cut(s) 222, 782, 998, 1007, 1019, 1028, 1034
HpyF3I CTNAG 2 cut(s) 270, 408
HpySE526I ACGT 2 cut(s) 132, 237
Hsp92I GRCGYC 2 cut(s) 93, 132
Hsp92II CATG 5 cut(s) 52, 626, 856, 875, 1049
HspAI GCGC 2 cut(s) 256, 319
KroI GCCGGC 1 cut(s) 785
KroNI GCCGGC 1 cut(s) 787
KspI CCGCGG 1 cut(s) 157
Kzo9I GATC 4 cut(s) 187, 907, 1065, 1123
LmnI GCTCC 4 cut(s) 45, 271, 326, 652
Lsp1109I GCAGC 5 cut(s) 644, 1001, 1018, 1021, 1067
LweI GCATC 6 cut(s) 23, 470, 559, 763, 772, 1010
MaeI CTAG 2 cut(s) 672, 1041
MaeII ACGT 2 cut(s) 132, 237
MaeIII GTNAC 3 cut(s) 361, 813, 943
MalI GATC 4 cut(s) 189, 909, 1067, 1125
MboI GATC 4 cut(s) 187, 907, 1065, 1123
MboII GAAGA 6 cut(s) 29, 67, 127, 187, 190, 1118
MhlI GDGCHC 3 cut(s) 42, 218, 268
MluCI AATT 3 cut(s) 209, 280, 334
MlyI GAGTC 4 cut(s) 158, 485, 551, 1135
MmeI TCCRAC 1 cut(s) 255
Mph1103I ATGCAT 2 cut(s) 463, 778
MroNI GCCGGC 1 cut(s) 785
MseI TTAA 1 cut(s) 1134
MslI CAYNNNNRTG 2 cut(s) 50, 1102
MspA1I CMGCKG 1 cut(s) 156
MspI CCGG 1 cut(s) 786
MspR9I CCNGG 3 cut(s) 179, 831, 864
Mva1269I GAATGC 1 cut(s) 1024
MvaI CCWGG 3 cut(s) 179, 831, 864
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 7 cut(s) 222, 782, 998, 1007, 1019, 1028, 1034
NaeI GCCGGC 1 cut(s) 787
NcoI CCATGG 2 cut(s) 48, 871
NdeI CATATG 2 cut(s) 778, 1120
NdeII GATC 4 cut(s) 187, 907, 1065, 1123
NgoMIV GCCGGC 1 cut(s) 785
NlaIII CATG 5 cut(s) 52, 626, 856, 875, 1049
NlaIV GGNNCC 4 cut(s) 114, 267, 654, 806
NmuCI GTSAC 2 cut(s) 361, 813
NsbI TGCGCA 1 cut(s) 257
NsiI ATGCAT 2 cut(s) 463, 778
OliI CACNNNNGTG 1 cut(s) 50
PaqCI CACCTGC 2 cut(s) 249, 526
PcsI WCGNNNNNNNCGW 4 cut(s) 96, 135, 189, 198
PctI GAATGC 1 cut(s) 1024
PdiI GCCGGC 1 cut(s) 787
PfeI GAWTC 2 cut(s) 397, 960
PleI GAGTC 4 cut(s) 158, 485, 551, 1135
PpsI GAGTC 4 cut(s) 158, 485, 551, 1135
PpuMI RGGWCCY 3 cut(s) 388, 860, 929
PshAI GACNNNNGTC 1 cut(s) 927
Psp124BI GAGCTC 2 cut(s) 42, 218
Psp5II RGGWCCY 3 cut(s) 388, 860, 929
Psp6I CCWGG 3 cut(s) 177, 829, 862
PspFI CCCAGC 2 cut(s) 73, 467
PspGI CCWGG 3 cut(s) 177, 829, 862
PspN4I GGNNCC 4 cut(s) 114, 267, 654, 806
PspPI GGNCC 4 cut(s) 112, 388, 860, 929
PspPPI RGGWCCY 3 cut(s) 388, 860, 929
PstNI CAGNNNCTG 1 cut(s) 995
RsaI GTAC 2 cut(s) 768, 851
RsaNI GTAC 2 cut(s) 767, 850
RseI CAYNNNNRTG 2 cut(s) 50, 1102
SacI GAGCTC 2 cut(s) 42, 218
SacII CCGCGG 1 cut(s) 157
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 1 cut(s) 1134
Sau3AI GATC 4 cut(s) 187, 907, 1065, 1123
Sau96I GGNCC 4 cut(s) 112, 388, 860, 929
SchI GAGTC 4 cut(s) 158, 485, 551, 1135
ScrFI CCNGG 3 cut(s) 179, 831, 864
SduI GDGCHC 3 cut(s) 42, 218, 268
SfaNI GCATC 6 cut(s) 23, 470, 559, 763, 772, 1010
Sfr303I CCGCGG 1 cut(s) 157
SgrBI CCGCGG 1 cut(s) 157
SgrDI CGTCGACG 1 cut(s) 89
SinI GGWCC 4 cut(s) 112, 388, 860, 929
SmiMI CAYNNNNRTG 2 cut(s) 50, 1102
Sse9I AATT 3 cut(s) 209, 280, 334
SsiI CCGC 9 cut(s) 118, 154, 156, 169, 172, 175, 242, 1010, 1028
SspMI CTAG 2 cut(s) 672, 1041
SstI GAGCTC 2 cut(s) 42, 218
StyD4I CCNGG 3 cut(s) 177, 829, 862
StyI CCWWGG 2 cut(s) 48, 871
TaaI ACNGT 7 cut(s) 14, 85, 361, 367, 454, 849, 1104
TaiI ACGT 2 cut(s) 135, 240
TaqI TCGA 4 cut(s) 90, 105, 138, 796
TasI AATT 3 cut(s) 209, 280, 334
TatI WGTACW 1 cut(s) 849
TauI GCSGC 5 cut(s) 159, 174, 177, 1013, 1031
TfiI GAWTC 2 cut(s) 397, 960
Tru1I TTAA 1 cut(s) 1134
Tru9I TTAA 1 cut(s) 1134
TscAI CASTG 1 cut(s) 1155
TseFI GTSAC 2 cut(s) 361, 813
TseI GCWGC 5 cut(s) 632, 989, 1031, 1034, 1080
Tsp45I GTSAC 2 cut(s) 361, 813
TspDTI ATGAA 3 cut(s) 473, 869, 1086
TspRI CASTG 1 cut(s) 1155
VpaK11BI GGWCC 4 cut(s) 112, 388, 860, 929
XapI RAATTY 1 cut(s) 334
XmiI GTMKAC 1 cut(s) 90
XspI CTAG 2 cut(s) 672, 1041
ZraI GACGTC 1 cut(s) 133
Zsp2I ATGCAT 2 cut(s) 463, 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.