MD08G1078900.v1.1

protein KIAA0930 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004749
Physical Location & Seq
Reverse (-)
6577051 .. 6581037
3987 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1078900.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGCTTCCAATCGAAATGCTCGGACATGGAGAAGACACCCCCTCCAGATTCGAATTGCTGAGTATGGTGAAGAAGCACTCGAGCTTATTGGGGAAAACAGTGATCGTCGCGGACGAGGACGACACTTCCGATGTCGAATTGGACCACAGATTCTGGCACGACGTGTTGGATTTTTACTTCGTTCGCGGTAAAGATTCCAGAGGACGCCAGGACGATGATCTCGTCTTCTTCGTCAGAAAATTGATCTCTTATGGGTATGGATTCAGTGACGGCAAGGAAGGTGTTTCTCCGTACTTTGTACGCAGGTGGGCACCTAAGTTGGATGACTTGATCGGGGAAAGTAATGTGGATGTGGATTGGAGGCGTACGTTTTACTTGAACTTGATTGCTCACACTTCTTTTACTGTAACAGTGGCTATTTGCAGTCATCAGGACCTCCGGAATCATCAAGCACAGCAAGGTATACCATTATCTCCAATATATAAGGTTGTAAAGACTGTTTATGCATCTCCAAGCCGTGTCAATTTTCAGTTGGACTCAAAAAAGGAAGTGGAGACAACACCTGCTTATCCAGATATTTGTTTTGCAATCGATGACTTCGACTCCACTTTTGATGCTGTGGTCTTGACTGAAACAGACCATTGCTACTGTGTAATTCTCAATGCACACGATGGGGCAGCATTTCCGAGGGAAAAGGAGTCTAATGATTGCAGTCCTAGTGATAGTTCCTCTCTGACAGTTGAGACTAACTCCGCACAGATGAAAAATACTAAGCTGACTCTTTTTTCAGGATTCGTCAGCTATCAAATGGTTCGAGACGCATATGATGCTGGCAAGTCTCGATTTGGGAACCTTCTTGGTCATTCCCCTGGCAAAACAGACAAACTTTACATGAAAGGTCCCGGAGGACGTGGGGAAGTTGAAGTAGCGGTTTCTAGGGTTGCAGATCAAAGCCAACAAGATCTGGGTCCTTTTTCACCAGTTATATCCAAAAGAGGATTTGGAATTGGCTCCATTGTTCGTAAAGCAGCTTCTGTTGCATCTGTGGCTGCAAAGAATGCCTATGCAGCTGCTTCTTCTACCTATAGTTTTGATGATGAGATGGTACCTCTCAAATGCTGCTTAATGTCCATTTCATTACCCTGGGAACAGATTGCATATGATCTTCTCTTTAAGGGTACTCCTCCAGTGACGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2045

Protein Analysis

400

Amino Acids

44.28

Weight (kDa)

5.41

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2045 PF09741 48 - 314 8.8e-77 Uncharacterized conserved protein (DUF2045)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 294, 571
Acc36I ACCTGC 2 cut(s) 294, 571
Acc65I GGTACC 1 cut(s) 1105
AccB1I GGYRCC 2 cut(s) 310, 1105
AccI GTMKAC 1 cut(s) 463
AccII CGCG 2 cut(s) 110, 186
AccIII TCCGGA 1 cut(s) 438
AciI CCGC 4 cut(s) 110, 186, 753, 929
AcyI GRCGYC 1 cut(s) 205
AdeI CACNNNGTG 1 cut(s) 163
AfaI GTAC 5 cut(s) 293, 300, 367, 1107, 1180
AflIII ACRYGT 1 cut(s) 162
AgsI TTSAA 2 cut(s) 379, 923
AjiI CACGTC 2 cut(s) 163, 911
AjnI CCWGG 3 cut(s) 207, 868, 1142
AluBI AGCT 5 cut(s) 84, 775, 801, 1031, 1070
AluI AGCT 5 cut(s) 84, 775, 801, 1031, 1070
Alw26I GTCTC 4 cut(s) 548, 737, 810, 843
AlwNI CAGNNNCTG 2 cut(s) 153, 1034
Ama87I CYCGRG 1 cut(s) 79
Aor13HI TCCGGA 1 cut(s) 438
ApeKI GCWGC 6 cut(s) 677, 1028, 1049, 1067, 1070, 1119
ArsI GACNNNNNNTTYG 2 cut(s) 593, 625
Asp718I GGTACC 1 cut(s) 1105
AspS9I GGNCC 4 cut(s) 142, 433, 899, 968
AsuC2I CCSGG 1 cut(s) 903
AsuHPI GGTGA 2 cut(s) 79, 969
AsuII TTCGAA 1 cut(s) 51
AvaI CYCGRG 1 cut(s) 79
AvaII GGWCC 4 cut(s) 142, 433, 899, 968
BaeGI GKGCMC 1 cut(s) 313
BanI GGYRCC 2 cut(s) 310, 1105
BarI GAAGNNNNNNTAC 2 cut(s) 1015, 1047
BbsI GAAGAC 2 cut(s) 39, 217
BbvI GCAGC 6 cut(s) 689, 1036, 1040, 1057, 1079, 1106
BccI CCATC 2 cut(s) 665, 1096
BceAI ACGGC 2 cut(s) 286, 501
BciT130I CCWGG 3 cut(s) 209, 870, 1144
BcnI CCSGG 1 cut(s) 903
BcoDI GTCTC 4 cut(s) 548, 737, 810, 843
BfaI CTAG 2 cut(s) 717, 936
BfmI CTRYAG 1 cut(s) 1084
BfuAI ACCTGC 2 cut(s) 294, 571
BglII AGATCT 1 cut(s) 961
BisI GCNGC 6 cut(s) 678, 1029, 1050, 1068, 1071, 1120
BlsI GCNGC 6 cut(s) 679, 1030, 1051, 1069, 1072, 1121
Bme1390I CCNGG 4 cut(s) 209, 870, 903, 1144
Bme18I GGWCC 4 cut(s) 142, 433, 899, 968
BmeT110I CYCGRG 1 cut(s) 79
BmgBI CACGTC 2 cut(s) 163, 911
BmgT120I GGNCC 4 cut(s) 142, 433, 899, 968
BmiI GGNNCC 6 cut(s) 312, 851, 901, 969, 1012, 1107
BmrFI CCNGG 4 cut(s) 209, 870, 903, 1144
BmsI GCATC 4 cut(s) 515, 604, 817, 1049
BpiI GAAGAC 2 cut(s) 39, 217
BplI GAGNNNNNCTC 2 cut(s) 734, 766
BpmI CTGGAG 2 cut(s) 28, 1170
Bpu14I TTCGAA 1 cut(s) 51
BpuMI CCSGG 1 cut(s) 903
Bsa29I ATCGAT 1 cut(s) 591
BsaHI GRCGYC 1 cut(s) 205
BsaJI CCNNGG 4 cut(s) 686, 868, 1142, 1143
BsaWI WCCGGW 1 cut(s) 438
BsaXI ACNNNNNCTCC 4 cut(s) 26, 56, 587, 617
Bse1I ACTGG 2 cut(s) 980, 1187
Bse3DI GCAATG 1 cut(s) 640
BseAI TCCGGA 1 cut(s) 438
BseBI CCWGG 3 cut(s) 209, 870, 1144
BseCI ATCGAT 1 cut(s) 591
BseDI CCNNGG 4 cut(s) 686, 868, 1142, 1143
BseGI GGATG 2 cut(s) 328, 355
BseMI GCAATG 1 cut(s) 640
BseMII CTCAG 1 cut(s) 50
BseNI ACTGG 2 cut(s) 980, 1187
BseRI GAGGAG 1 cut(s) 1173
BseSI GKGCMC 1 cut(s) 313
BseXI GCAGC 6 cut(s) 689, 1036, 1040, 1057, 1079, 1106
Bsh1236I CGCG 2 cut(s) 110, 186
BshNI GGYRCC 2 cut(s) 310, 1105
BshVI ATCGAT 1 cut(s) 591
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 2 cut(s) 439, 903
BsiWI CGTACG 1 cut(s) 365
BslFI GGGAC 1 cut(s) 885
BsmAI GTCTC 4 cut(s) 548, 737, 810, 843
BsmBI CGTCTC 1 cut(s) 810
BsmFI GGGAC 1 cut(s) 885
BsmI GAATGC 1 cut(s) 1063
BsoBI CYCGRG 1 cut(s) 79
Bsp119I TTCGAA 1 cut(s) 51
Bsp1286I GDGCHC 1 cut(s) 313
Bsp13I TCCGGA 1 cut(s) 438
Bsp143I GATC 7 cut(s) 102, 217, 243, 330, 946, 961, 1162
BspACI CCGC 4 cut(s) 110, 186, 753, 929
BspCNI CTCAG 1 cut(s) 51
BspDI ATCGAT 1 cut(s) 591
BspEI TCCGGA 1 cut(s) 438
BspFNI CGCG 2 cut(s) 110, 186
BspLI GGNNCC 6 cut(s) 312, 851, 901, 969, 1012, 1107
BspMI ACCTGC 2 cut(s) 294, 571
BspT104I TTCGAA 1 cut(s) 51
BspT107I GGYRCC 2 cut(s) 310, 1105
BsrDI GCAATG 1 cut(s) 640
BsrI ACTGG 2 cut(s) 980, 1187
BssECI CCNNGG 4 cut(s) 686, 868, 1142, 1143
BssMI GATC 7 cut(s) 102, 217, 243, 330, 946, 961, 1162
BssNAI GTATAC 1 cut(s) 464
BssNI GRCGYC 1 cut(s) 205
Bst1107I GTATAC 1 cut(s) 464
Bst2UI CCWGG 3 cut(s) 209, 870, 1144
Bst4CI ACNGT 6 cut(s) 100, 406, 412, 499, 650, 739
BstACI GRCGYC 1 cut(s) 205
BstAPI GCANNNNNTGC 2 cut(s) 827, 1058
BstBI TTCGAA 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 832
BstDEI CTNAG 3 cut(s) 59, 315, 771
BstF5I GGATG 2 cut(s) 328, 355
BstFNI CGCG 2 cut(s) 110, 186
BstKTI GATC 7 cut(s) 105, 220, 246, 333, 949, 964, 1165
BstMAI GTCTC 4 cut(s) 548, 737, 810, 843
BstMBI GATC 7 cut(s) 102, 217, 243, 330, 946, 961, 1162
BstMWI GCNNNNNNNGC 5 cut(s) 827, 1037, 1046, 1058, 1067
BstNI CCWGG 3 cut(s) 209, 870, 1144
BstSCI CCNGG 4 cut(s) 207, 868, 901, 1142
BstSFI CTRYAG 1 cut(s) 1084
BstSLI GKGCMC 1 cut(s) 313
BstUI CGCG 2 cut(s) 110, 186
BstV1I GCAGC 6 cut(s) 689, 1036, 1040, 1057, 1079, 1106
BstV2I GAAGAC 2 cut(s) 39, 217
BstX2I RGATCY 1 cut(s) 961
BstYI RGATCY 1 cut(s) 961
BstZ17I GTATAC 1 cut(s) 464
Bsu15I ATCGAT 1 cut(s) 591
BsuTUI ATCGAT 1 cut(s) 591
BtrI CACGTC 2 cut(s) 163, 911
BtsCI GGATG 2 cut(s) 328, 355
BtsIMutI CAGTG 4 cut(s) 105, 271, 417, 1194
BveI ACCTGC 2 cut(s) 294, 571
Cac8I GCNNGC 1 cut(s) 832
CaiI CAGNNNCTG 2 cut(s) 153, 1034
Cfr13I GGNCC 4 cut(s) 142, 433, 899, 968
ClaI ATCGAT 1 cut(s) 591
CseI GACGC 2 cut(s) 213, 827
Csp6I GTAC 5 cut(s) 292, 299, 366, 1106, 1179
CviAII CATG 2 cut(s) 26, 892
CviQI GTAC 5 cut(s) 292, 299, 366, 1106, 1179
DdeI CTNAG 3 cut(s) 59, 315, 771
DpnI GATC 7 cut(s) 104, 219, 245, 332, 948, 963, 1164
DpnII GATC 7 cut(s) 102, 217, 243, 330, 946, 961, 1162
DraIII CACNNNGTG 1 cut(s) 163
Eco47I GGWCC 4 cut(s) 142, 433, 899, 968
Eco88I CYCGRG 1 cut(s) 79
EcoO109I RGGNCCY 3 cut(s) 433, 899, 968
EcoRII CCWGG 3 cut(s) 207, 868, 1142
EcoT22I ATGCAT 1 cut(s) 508
Esp3I CGTCTC 1 cut(s) 810
FaeI CATG 2 cut(s) 29, 895
FaqI GGGAC 1 cut(s) 885
FatI CATG 2 cut(s) 25, 891
FauNDI CATATG 2 cut(s) 823, 1159
FblI GTMKAC 1 cut(s) 463
Fnu4HI GCNGC 6 cut(s) 678, 1029, 1050, 1068, 1071, 1120
FokI GGATG 2 cut(s) 335, 362
Fsp4HI GCNGC 6 cut(s) 678, 1029, 1050, 1068, 1071, 1120
FspBI CTAG 2 cut(s) 717, 936
GluI GCNGC 6 cut(s) 678, 1029, 1050, 1068, 1071, 1120
GsuI CTGGAG 2 cut(s) 28, 1170
HapII CCGG 2 cut(s) 439, 903
HgaI GACGC 2 cut(s) 213, 827
Hin1I GRCGYC 1 cut(s) 205
Hin1II CATG 2 cut(s) 29, 895
HpaII CCGG 2 cut(s) 439, 903
HphI GGTGA 2 cut(s) 79, 969
Hpy166II GTNNAC 1 cut(s) 464
Hpy188I TCNGA 5 cut(s) 23, 130, 236, 687, 735
Hpy188III TCNNGA 9 cut(s) 45, 198, 431, 439, 572, 625, 789, 815, 840
Hpy8I GTNNAC 1 cut(s) 464
Hpy99I CGWCG 2 cut(s) 110, 164
HpyAV CCTTC 2 cut(s) 272, 863
HpyCH4III ACNGT 6 cut(s) 100, 406, 412, 499, 650, 739
HpyCH4IV ACGT 4 cut(s) 162, 368, 910, 1193
HpyF10VI GCNNNNNNNGC 5 cut(s) 827, 1037, 1046, 1058, 1067
HpyF3I CTNAG 3 cut(s) 59, 315, 771
HpySE526I ACGT 4 cut(s) 162, 368, 910, 1193
Hsp92I GRCGYC 1 cut(s) 205
Hsp92II CATG 2 cut(s) 29, 895
Kpn2I TCCGGA 1 cut(s) 438
KpnI GGTACC 1 cut(s) 1109
Kzo9I GATC 7 cut(s) 102, 217, 243, 330, 946, 961, 1162
LmnI GCTCC 1 cut(s) 1016
Lsp1109I GCAGC 6 cut(s) 689, 1036, 1040, 1057, 1079, 1106
LweI GCATC 4 cut(s) 515, 604, 817, 1049
MaeI CTAG 2 cut(s) 717, 936
MaeII ACGT 4 cut(s) 162, 368, 910, 1193
MaeIII GTNAC 3 cut(s) 266, 406, 1189
MalI GATC 7 cut(s) 104, 219, 245, 332, 948, 963, 1164
MboI GATC 7 cut(s) 102, 217, 243, 330, 946, 961, 1162
MboII GAAGA 6 cut(s) 44, 82, 217, 220, 1068, 1157
MflI RGATCY 1 cut(s) 961
MhlI GDGCHC 1 cut(s) 313
MluCI AATT 6 cut(s) 53, 137, 239, 523, 654, 1005
MlyI GAGTC 4 cut(s) 530, 596, 707, 772
MmeI TCCRAC 3 cut(s) 147, 300, 513
Mph1103I ATGCAT 1 cut(s) 508
MroI TCCGGA 1 cut(s) 438
MseI TTAA 2 cut(s) 1124, 1173
MspA1I CMGCKG 1 cut(s) 1070
MspI CCGG 2 cut(s) 439, 903
MspR9I CCNGG 4 cut(s) 209, 870, 903, 1144
Mva1269I GAATGC 1 cut(s) 1063
MvaI CCWGG 3 cut(s) 209, 870, 1144
MvnI CGCG 2 cut(s) 110, 186
MwoI GCNNNNNNNGC 5 cut(s) 827, 1037, 1046, 1058, 1067
NciI CCSGG 1 cut(s) 903
NdeI CATATG 2 cut(s) 823, 1159
NdeII GATC 7 cut(s) 102, 217, 243, 330, 946, 961, 1162
NlaIII CATG 2 cut(s) 29, 895
NlaIV GGNNCC 6 cut(s) 312, 851, 901, 969, 1012, 1107
NmuCI GTSAC 2 cut(s) 266, 1189
NsiI ATGCAT 1 cut(s) 508
NspV TTCGAA 1 cut(s) 51
PaeR7I CTCGAG 1 cut(s) 79
PaqCI CACCTGC 2 cut(s) 294, 571
PasI CCCWGGG 1 cut(s) 1143
PcsI WCGNNNNNNNCGW 5 cut(s) 111, 126, 219, 228, 597
PctI GAATGC 1 cut(s) 1063
PfeI GAWTC 6 cut(s) 48, 150, 194, 261, 442, 792
Pfl23II CGTACG 1 cut(s) 365
PfoI TCCNGGA 1 cut(s) 901
PkrI GCNGC 6 cut(s) 679, 1030, 1051, 1069, 1072, 1121
PleI GAGTC 4 cut(s) 530, 596, 706, 772
PpsI GAGTC 4 cut(s) 530, 596, 706, 772
PpuMI RGGWCCY 3 cut(s) 433, 899, 968
Psp5II RGGWCCY 3 cut(s) 433, 899, 968
Psp6I CCWGG 3 cut(s) 207, 868, 1142
PspGI CCWGG 3 cut(s) 207, 868, 1142
PspLI CGTACG 1 cut(s) 365
PspN4I GGNNCC 6 cut(s) 312, 851, 901, 969, 1012, 1107
PspPI GGNCC 4 cut(s) 142, 433, 899, 968
PspPPI RGGWCCY 3 cut(s) 433, 899, 968
PspXI VCTCGAGB 1 cut(s) 79
PstNI CAGNNNCTG 2 cut(s) 153, 1034
PsuI RGATCY 1 cut(s) 961
PvuII CAGCTG 1 cut(s) 1070
RsaI GTAC 5 cut(s) 293, 300, 367, 1107, 1180
RsaNI GTAC 5 cut(s) 292, 299, 366, 1106, 1179
SaqAI TTAA 2 cut(s) 1124, 1173
SatI GCNGC 6 cut(s) 678, 1029, 1050, 1068, 1071, 1120
Sau3AI GATC 7 cut(s) 102, 217, 243, 330, 946, 961, 1162
Sau96I GGNCC 4 cut(s) 142, 433, 899, 968
SchI GAGTC 4 cut(s) 530, 596, 707, 772
ScrFI CCNGG 4 cut(s) 209, 870, 903, 1144
SduI GDGCHC 1 cut(s) 313
SfaNI GCATC 4 cut(s) 515, 604, 817, 1049
SfcI CTRYAG 1 cut(s) 1084
Sfr274I CTCGAG 1 cut(s) 79
SfuI TTCGAA 1 cut(s) 51
SinI GGWCC 4 cut(s) 142, 433, 899, 968
SlaI CTCGAG 1 cut(s) 79
SmlI CTYRAG 1 cut(s) 79
SmoI CTYRAG 1 cut(s) 79
Sse9I AATT 6 cut(s) 53, 137, 239, 523, 654, 1005
SsiI CCGC 4 cut(s) 110, 186, 753, 929
SspMI CTAG 2 cut(s) 717, 936
StyD4I CCNGG 4 cut(s) 207, 868, 901, 1142
TaaI ACNGT 6 cut(s) 100, 406, 412, 499, 650, 739
TaiI ACGT 4 cut(s) 165, 371, 913, 1196
TaqI TCGA 8 cut(s) 12, 51, 80, 135, 591, 600, 814, 841
TasI AATT 6 cut(s) 53, 137, 239, 523, 654, 1005
TfiI GAWTC 6 cut(s) 48, 150, 194, 261, 442, 792
Tru1I TTAA 2 cut(s) 1124, 1173
Tru9I TTAA 2 cut(s) 1124, 1173
TscAI CASTG 4 cut(s) 105, 271, 417, 1194
TseFI GTSAC 2 cut(s) 266, 1189
TseI GCWGC 6 cut(s) 677, 1028, 1049, 1067, 1070, 1119
Tsp45I GTSAC 2 cut(s) 266, 1189
TspDTI ATGAA 3 cut(s) 776, 908, 1125
TspGWI ACGGA 1 cut(s) 279
TspRI CASTG 4 cut(s) 105, 271, 417, 1194
VpaK11BI GGWCC 4 cut(s) 142, 433, 899, 968
XhoI CTCGAG 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 463
XspI CTAG 2 cut(s) 717, 936
Zsp2I ATGCAT 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.