MD15G1065900.v1.1

protein KIAA0930 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004749
Physical Location & Seq
Reverse (-)
4586174 .. 4590319
4146 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1065900.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGCTCGGTCATGGAGAAGAAACCCCCTCCAGATTTGAATTGCTGAGTATGGTGAAGAAGCACTCGAACTTGTTGGGGAAAACAGTGATAGTCGCGGACGAGGACGACACTTCCGATGTCGAATTGGACCACAGATTCTGGCACGATATGTTGGATTTGTACTTCGTACGCGGTAAAGATTCCAGAGGACGTCAGGACGATGATCTCGTCTTCTTCGTCAGAAAATTGAGCTATTATGGGTATGGATTTAATGACGGCAAGGAAGGTGTTTCTCCGTACTTTGTACGCAGGTGGGCACATAAGTTGGATGACTTGATTGGGGAAAGTAATGTGGATGTGGATTGGAGGCGTTCGTTTTACTTGAACTTGATTGCTCACACTTCATTCACTGTAACAGTGGCTATTTGCAGTCATCAGGACCTTCGGAATCATCAAGCTGAGCAAGGTATACCATTATCTCCAATATATAAGGTTGTAAAGACTGTTTATGCATCTCCAAGCCGTGTCGATTTTCAGTTGGACTCGAAAAAGGAAGTGGAGACAACACCTGCTTATCCAGATATTTGTTTTGCAATCGATGACTTTGACTCCACTTTTGATGCTGTGGTCTTGACTGAAACAGACCATTGCTACTGTGTAATTCTCAATGCACACGATGGGGCAGCATTTCCTAGGGTAAGGGAGGTGGATGAATGCAGTTCTAGTGATAGTTCCTCTTTGAGGATTGAGACAAATTCTGCAAAGACGAAAAATACTAAGCTGACTCTTTTTTCAGGATTCGTTAGCTATCAAATGGTCCGAGATGCATATGATGCTGGCAAGTCTCGATTTGGGAATCTGCTTGGTCATTCCCCTGGCAAAACAGACAAACTTTACATGAAAGGTCCCGGAGGACGTGGGGAAGTTGAAGTAGCTGTTTCTGGTGTTGCAGATCAAAGCCAACAAGATCTGGGTCCTTTTTCACCTGTTATATCCAAGACAGGATTCGGGATTGGCTCCATTGTTCGCAAAGCAGCTTCTGTTGCATCTGTGGCTGCGAAGAATGCCTATGCAGCTGCTTCTTCTACCCATAGTTTTGATGATGAGATGGTACCTCTCAAATGTTGCTTAATGTCCATTTCATTACCCTGGGAACAGATTGCACATGATCTTCTCTTTAAGGGTACTCCTCCAGTGACATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2045

Protein Analysis

395

Amino Acids

43.69

Weight (kDa)

5.54

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2045 PF09741 43 - 309 9.5e-75 Uncharacterized conserved protein (DUF2045)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 279, 556
AatII GACGTC 1 cut(s) 193
Acc36I ACCTGC 2 cut(s) 279, 556
Acc65I GGTACC 1 cut(s) 1090
AccB1I GGYRCC 1 cut(s) 1090
AccI GTMKAC 1 cut(s) 448
AccII CGCG 2 cut(s) 95, 171
AciI CCGC 2 cut(s) 95, 171
AcsI RAATTY 1 cut(s) 733
AcyI GRCGYC 1 cut(s) 190
AfaI GTAC 6 cut(s) 161, 168, 278, 285, 1092, 1165
AfiI CCNNNNNNNGG 1 cut(s) 720
AgsI TTSAA 3 cut(s) 38, 364, 908
AjiI CACGTC 1 cut(s) 896
AjnI CCWGG 2 cut(s) 853, 1127
AjuI GAANNNNNNNTTGG 2 cut(s) 968, 1000
AluBI AGCT 7 cut(s) 231, 437, 760, 786, 914, 1016, 1055
AluI AGCT 7 cut(s) 231, 437, 760, 786, 914, 1016, 1055
Alw26I GTCTC 3 cut(s) 533, 722, 828
AlwNI CAGNNNCTG 2 cut(s) 138, 1019
ApeKI GCWGC 5 cut(s) 662, 1013, 1034, 1052, 1055
ApoI RAATTY 1 cut(s) 733
ArsI GACNNNNNNTTYG 2 cut(s) 578, 610
Asp718I GGTACC 1 cut(s) 1090
AspA2I CCTAGG 1 cut(s) 671
AspS9I GGNCC 5 cut(s) 127, 418, 796, 884, 953
AsuC2I CCSGG 1 cut(s) 888
AsuHPI GGTGA 2 cut(s) 64, 954
AvaII GGWCC 5 cut(s) 127, 418, 796, 884, 953
AvrII CCTAGG 1 cut(s) 671
BaeGI GKGCMC 1 cut(s) 298
BanI GGYRCC 1 cut(s) 1090
BbsI GAAGAC 1 cut(s) 202
BbvI GCAGC 5 cut(s) 674, 1021, 1025, 1042, 1064
BccI CCATC 2 cut(s) 650, 1081
BceAI ACGGC 2 cut(s) 271, 486
BciT130I CCWGG 2 cut(s) 855, 1129
BcnI CCSGG 1 cut(s) 888
BcoDI GTCTC 3 cut(s) 533, 722, 828
BfaI CTAG 2 cut(s) 672, 702
BfuAI ACCTGC 2 cut(s) 279, 556
BglII AGATCT 1 cut(s) 946
BisI GCNGC 5 cut(s) 663, 1014, 1035, 1053, 1056
BlnI CCTAGG 1 cut(s) 671
BlpI GCTNAGC 1 cut(s) 438
BlsI GCNGC 5 cut(s) 664, 1015, 1036, 1054, 1057
Bme1390I CCNGG 3 cut(s) 855, 888, 1129
Bme18I GGWCC 5 cut(s) 127, 418, 796, 884, 953
BmgBI CACGTC 1 cut(s) 896
BmgT120I GGNCC 5 cut(s) 127, 418, 796, 884, 953
BmiI GGNNCC 4 cut(s) 886, 954, 997, 1092
BmrFI CCNGG 3 cut(s) 855, 888, 1129
BmsI GCATC 5 cut(s) 500, 589, 793, 802, 1034
BpiI GAAGAC 1 cut(s) 202
BpmI CTGGAG 2 cut(s) 13, 1155
Bpu1102I GCTNAGC 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 888
Bsa29I ATCGAT 1 cut(s) 576
BsaHI GRCGYC 1 cut(s) 190
BsaJI CCNNGG 4 cut(s) 671, 853, 1127, 1128
BsaXI ACNNNNNCTCC 2 cut(s) 572, 602
Bsc4I CCNNNNNNNGG 1 cut(s) 720
Bse1I ACTGG 1 cut(s) 1172
Bse3DI GCAATG 1 cut(s) 625
BseBI CCWGG 2 cut(s) 855, 1129
BseCI ATCGAT 1 cut(s) 576
BseDI CCNNGG 4 cut(s) 671, 853, 1127, 1128
BseGI GGATG 3 cut(s) 313, 340, 694
BseLI CCNNNNNNNGG 1 cut(s) 720
BseMI GCAATG 1 cut(s) 625
BseMII CTCAG 2 cut(s) 35, 429
BseNI ACTGG 1 cut(s) 1172
BseRI GAGGAG 1 cut(s) 1158
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 5 cut(s) 674, 1021, 1025, 1042, 1064
Bsh1236I CGCG 2 cut(s) 95, 171
BshNI GGYRCC 1 cut(s) 1090
BshVI ATCGAT 1 cut(s) 576
BsiSI CCGG 1 cut(s) 888
BsiWI CGTACG 1 cut(s) 166
BslFI GGGAC 1 cut(s) 870
BslI CCNNNNNNNGG 1 cut(s) 720
BsmAI GTCTC 3 cut(s) 533, 722, 828
BsmFI GGGAC 1 cut(s) 870
BsmI GAATGC 2 cut(s) 698, 1048
Bsp1286I GDGCHC 1 cut(s) 298
Bsp143I GATC 4 cut(s) 202, 931, 946, 1147
Bsp1720I GCTNAGC 1 cut(s) 438
BspACI CCGC 2 cut(s) 95, 171
BspCNI CTCAG 2 cut(s) 36, 430
BspDI ATCGAT 1 cut(s) 576
BspFNI CGCG 2 cut(s) 95, 171
BspLI GGNNCC 4 cut(s) 886, 954, 997, 1092
BspMI ACCTGC 2 cut(s) 279, 556
BspT107I GGYRCC 1 cut(s) 1090
BsrDI GCAATG 1 cut(s) 625
BsrI ACTGG 1 cut(s) 1172
BssECI CCNNGG 4 cut(s) 671, 853, 1127, 1128
BssMI GATC 4 cut(s) 202, 931, 946, 1147
BssNAI GTATAC 1 cut(s) 449
BssNI GRCGYC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 671
Bst1107I GTATAC 1 cut(s) 449
Bst2UI CCWGG 2 cut(s) 855, 1129
Bst4CI ACNGT 5 cut(s) 85, 391, 397, 484, 635
BstACI GRCGYC 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 812
BstC8I GCNNGC 1 cut(s) 817
BstDEI CTNAG 3 cut(s) 44, 438, 756
BstENI CCTNNNNNAGG 1 cut(s) 718
BstF5I GGATG 3 cut(s) 313, 340, 694
BstFNI CGCG 2 cut(s) 95, 171
BstKTI GATC 4 cut(s) 205, 934, 949, 1150
BstMAI GTCTC 3 cut(s) 533, 722, 828
BstMBI GATC 4 cut(s) 202, 931, 946, 1147
BstMWI GCNNNNNNNGC 5 cut(s) 812, 1022, 1031, 1043, 1052
BstNI CCWGG 2 cut(s) 855, 1129
BstSCI CCNGG 3 cut(s) 853, 886, 1127
BstSLI GKGCMC 1 cut(s) 298
BstUI CGCG 2 cut(s) 95, 171
BstV1I GCAGC 5 cut(s) 674, 1021, 1025, 1042, 1064
BstV2I GAAGAC 1 cut(s) 202
BstX2I RGATCY 1 cut(s) 946
BstYI RGATCY 1 cut(s) 946
BstZ17I GTATAC 1 cut(s) 449
Bsu15I ATCGAT 1 cut(s) 576
BsuTUI ATCGAT 1 cut(s) 576
BtrI CACGTC 1 cut(s) 896
BtsCI GGATG 3 cut(s) 313, 340, 694
BtsIMutI CAGTG 4 cut(s) 90, 387, 402, 1179
BveI ACCTGC 2 cut(s) 279, 556
Cac8I GCNNGC 1 cut(s) 817
CaiI CAGNNNCTG 2 cut(s) 138, 1019
Cfr13I GGNCC 5 cut(s) 127, 418, 796, 884, 953
ClaI ATCGAT 1 cut(s) 576
Csp6I GTAC 6 cut(s) 160, 167, 277, 284, 1091, 1164
CviAII CATG 3 cut(s) 11, 877, 1145
CviQI GTAC 6 cut(s) 160, 167, 277, 284, 1091, 1164
DdeI CTNAG 3 cut(s) 44, 438, 756
DpnI GATC 4 cut(s) 204, 933, 948, 1149
DpnII GATC 4 cut(s) 202, 931, 946, 1147
Eco130I CCWWGG 1 cut(s) 671
Eco47I GGWCC 5 cut(s) 127, 418, 796, 884, 953
EcoNI CCTNNNNNAGG 1 cut(s) 718
EcoO109I RGGNCCY 3 cut(s) 418, 884, 953
EcoRII CCWGG 2 cut(s) 853, 1127
EcoT14I CCWWGG 1 cut(s) 671
EcoT22I ATGCAT 2 cut(s) 493, 808
ErhI CCWWGG 1 cut(s) 671
FaeI CATG 3 cut(s) 14, 880, 1148
FaqI GGGAC 1 cut(s) 870
FatI CATG 3 cut(s) 10, 876, 1144
FauNDI CATATG 1 cut(s) 808
FblI GTMKAC 1 cut(s) 448
Fnu4HI GCNGC 5 cut(s) 663, 1014, 1035, 1053, 1056
FokI GGATG 3 cut(s) 320, 347, 701
Fsp4HI GCNGC 5 cut(s) 663, 1014, 1035, 1053, 1056
FspBI CTAG 2 cut(s) 672, 702
GluI GCNGC 5 cut(s) 663, 1014, 1035, 1053, 1056
GsuI CTGGAG 2 cut(s) 13, 1155
HapII CCGG 1 cut(s) 888
Hin1I GRCGYC 1 cut(s) 190
Hin1II CATG 3 cut(s) 14, 880, 1148
HinfI GANTC 9 cut(s) 135, 179, 427, 521, 587, 763, 777, 835, 984
HpaII CCGG 1 cut(s) 888
HphI GGTGA 2 cut(s) 64, 954
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 4 cut(s) 115, 221, 426, 800
Hpy188III TCNNGA 9 cut(s) 30, 183, 194, 416, 557, 610, 774, 825, 988
Hpy8I GTNNAC 1 cut(s) 449
HpyAV CCTTC 2 cut(s) 257, 431
HpyCH4III ACNGT 5 cut(s) 85, 391, 397, 484, 635
HpyCH4IV ACGT 2 cut(s) 190, 895
HpyF10VI GCNNNNNNNGC 5 cut(s) 812, 1022, 1031, 1043, 1052
HpyF3I CTNAG 3 cut(s) 44, 438, 756
HpySE526I ACGT 2 cut(s) 190, 895
Hsp92I GRCGYC 1 cut(s) 190
Hsp92II CATG 3 cut(s) 14, 880, 1148
KpnI GGTACC 1 cut(s) 1094
Kzo9I GATC 4 cut(s) 202, 931, 946, 1147
LmnI GCTCC 1 cut(s) 1001
Lsp1109I GCAGC 5 cut(s) 674, 1021, 1025, 1042, 1064
LweI GCATC 5 cut(s) 500, 589, 793, 802, 1034
MaeI CTAG 2 cut(s) 672, 702
MaeII ACGT 2 cut(s) 190, 895
MaeIII GTNAC 2 cut(s) 391, 1174
MalI GATC 4 cut(s) 204, 933, 948, 1149
MboI GATC 4 cut(s) 202, 931, 946, 1147
MboII GAAGA 7 cut(s) 29, 67, 202, 205, 1051, 1053, 1142
MflI RGATCY 1 cut(s) 946
MhlI GDGCHC 1 cut(s) 298
MluCI AATT 5 cut(s) 38, 122, 224, 639, 733
MlyI GAGTC 3 cut(s) 515, 581, 757
MmeI TCCRAC 3 cut(s) 132, 285, 498
Mph1103I ATGCAT 2 cut(s) 493, 808
MseI TTAA 3 cut(s) 249, 1109, 1158
MspA1I CMGCKG 1 cut(s) 1055
MspI CCGG 1 cut(s) 888
MspR9I CCNGG 3 cut(s) 855, 888, 1129
Mva1269I GAATGC 2 cut(s) 698, 1048
MvaI CCWGG 2 cut(s) 855, 1129
MvnI CGCG 2 cut(s) 95, 171
MwoI GCNNNNNNNGC 5 cut(s) 812, 1022, 1031, 1043, 1052
NciI CCSGG 1 cut(s) 888
NdeI CATATG 1 cut(s) 808
NdeII GATC 4 cut(s) 202, 931, 946, 1147
NlaIII CATG 3 cut(s) 14, 880, 1148
NlaIV GGNNCC 4 cut(s) 886, 954, 997, 1092
NmuCI GTSAC 1 cut(s) 1174
NsiI ATGCAT 2 cut(s) 493, 808
PaqCI CACCTGC 2 cut(s) 279, 556
PasI CCCWGGG 1 cut(s) 1128
PcsI WCGNNNNNNNCGW 3 cut(s) 111, 204, 213
PctI GAATGC 2 cut(s) 698, 1048
PfeI GAWTC 6 cut(s) 135, 179, 427, 777, 835, 984
Pfl23II CGTACG 1 cut(s) 166
PfoI TCCNGGA 1 cut(s) 886
PkrI GCNGC 5 cut(s) 664, 1015, 1036, 1054, 1057
PleI GAGTC 3 cut(s) 515, 581, 757
PpsI GAGTC 3 cut(s) 515, 581, 757
PpuMI RGGWCCY 3 cut(s) 418, 884, 953
Psp5II RGGWCCY 3 cut(s) 418, 884, 953
Psp6I CCWGG 2 cut(s) 853, 1127
PspGI CCWGG 2 cut(s) 853, 1127
PspLI CGTACG 1 cut(s) 166
PspN4I GGNNCC 4 cut(s) 886, 954, 997, 1092
PspPI GGNCC 5 cut(s) 127, 418, 796, 884, 953
PspPPI RGGWCCY 3 cut(s) 418, 884, 953
PstNI CAGNNNCTG 2 cut(s) 138, 1019
PsuI RGATCY 1 cut(s) 946
PvuII CAGCTG 1 cut(s) 1055
RsaI GTAC 6 cut(s) 161, 168, 278, 285, 1092, 1165
RsaNI GTAC 6 cut(s) 160, 167, 277, 284, 1091, 1164
SaqAI TTAA 3 cut(s) 249, 1109, 1158
SatI GCNGC 5 cut(s) 663, 1014, 1035, 1053, 1056
Sau3AI GATC 4 cut(s) 202, 931, 946, 1147
Sau96I GGNCC 5 cut(s) 127, 418, 796, 884, 953
SchI GAGTC 3 cut(s) 515, 581, 757
ScrFI CCNGG 3 cut(s) 855, 888, 1129
SduI GDGCHC 1 cut(s) 298
SfaNI GCATC 5 cut(s) 500, 589, 793, 802, 1034
SinI GGWCC 5 cut(s) 127, 418, 796, 884, 953
Sse9I AATT 5 cut(s) 38, 122, 224, 639, 733
SsiI CCGC 2 cut(s) 95, 171
SspMI CTAG 2 cut(s) 672, 702
StyD4I CCNGG 3 cut(s) 853, 886, 1127
StyI CCWWGG 1 cut(s) 671
TaaI ACNGT 5 cut(s) 85, 391, 397, 484, 635
TaiI ACGT 2 cut(s) 193, 898
TaqI TCGA 6 cut(s) 65, 120, 507, 524, 576, 826
TasI AATT 5 cut(s) 38, 122, 224, 639, 733
TatI WGTACW 1 cut(s) 159
TfiI GAWTC 6 cut(s) 135, 179, 427, 777, 835, 984
Tru1I TTAA 3 cut(s) 249, 1109, 1158
Tru9I TTAA 3 cut(s) 249, 1109, 1158
TscAI CASTG 4 cut(s) 90, 394, 402, 1179
TseFI GTSAC 1 cut(s) 1174
TseI GCWGC 5 cut(s) 662, 1013, 1034, 1052, 1055
Tsp45I GTSAC 1 cut(s) 1174
TspDTI ATGAA 4 cut(s) 372, 705, 893, 1110
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 4 cut(s) 90, 394, 402, 1179
VpaK11BI GGWCC 5 cut(s) 127, 418, 796, 884, 953
XagI CCTNNNNNAGG 1 cut(s) 718
XapI RAATTY 1 cut(s) 733
XmaJI CCTAGG 1 cut(s) 671
XmiI GTMKAC 1 cut(s) 448
XspI CTAG 2 cut(s) 672, 702
ZraI GACGTC 1 cut(s) 191
Zsp2I ATGCAT 2 cut(s) 493, 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.