pycom15g06230

protein KIAA0930 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004749
Physical Location & Seq
Reverse (-)
3828235 .. 3831869
3635 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g06230.2

Sequence Viewer

Length: 1074 bp
ATGCTCGGTCATGGAGAAGAAACCCCCTCCAGATTTGAATTGCTGAGTATGGTGAAGAAGCACTCGAGCTTGTTGGGGAAAACAGTGATAGTCGCGGACGAGGACGACACTTCCGATGTCGAATTGGACCACAGATTCTGGCACGATATGTTGGATTTGTACTTCGTACGCGGTAAAGATTCCAGAGGACGTCAGGACGATGATCTCGTCTTCTTCGTCAGAAAATTGAGCTCTTATGGGTATGGATTTAATGACGGCAAGGAAGGTGTTTCTCCGTACTTTGTACGCAGGTGGGCACATAAGTTGGATGACTTGATTGGGGAAAGTAATGTGGATGTGGATTGGAGGCGTTCGTTTTACTTGAATTTGATTGCTCACACTTCATTTACTGTAACAGTGGCTATTTGCAGTCATCAGGACCTTCGGAATCATCAAGCTGACCAAGGTATACCATTGTCTCCAATATATAAGGTTGTAAAAACTGTGTATGCATCTCCGAGCCGTGTCAATTTTCAGTTGGACTCGGAAAAGGAAGTGGAGACAACACCTGCTTATCCAGATATTTGTTTTGCAATCGATGACTTTGACTCCACTTTTGATGCTGTGGTCTTGACTGAAACAGACAATTGCTACTGCGTAATTCTCAATGCACATGATGGGGCAGCATTTCCTAGGGTAAAGGAGTCTGATGAATGCAGTTCTGGTGATAGTTCCTCTTTGAGGATTGAGACAAATTCTGCAAAGATGAAAAATACTAAGCTGACTCTTTTTTCAGGATTCGTCAGCTATCAAATGGTCCGAGATGCATATGATGCCGGTAAGTCACGATTTGGGAATCTGCTTGGTCATTCCCCTGGCAAAACAGACAAACTATACATGAAAGGTCCCGGAGGACGTGGGGAAGTTGAAGTAGCTGTTTCCGGTGTTGCAGATCAAAGCCAACATAGATCTGGGTCCTTTTTCACCTGTTATATCCAAGACAGGATTTGGGATTGGCTCCATTGTTCGCAAAGCAGCTTCTGTTGCATCTGTGGCTGCGAAGAATGCCTATGCAGCTGCTTCTTCTACCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2045

Protein Analysis

358

Amino Acids

40.23

Weight (kDa)

5.22

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 279, 556
AatII GACGTC 1 cut(s) 193
Acc36I ACCTGC 2 cut(s) 279, 556
AccI GTMKAC 1 cut(s) 448
AccII CGCG 2 cut(s) 95, 171
AciI CCGC 2 cut(s) 95, 171
AcsI RAATTY 2 cut(s) 364, 733
AcyI GRCGYC 1 cut(s) 190
AfaI GTAC 4 cut(s) 161, 168, 278, 285
AfiI CCNNNNNNNGG 1 cut(s) 720
AgsI TTSAA 3 cut(s) 38, 364, 908
AjiI CACGTC 1 cut(s) 896
AjnI CCWGG 1 cut(s) 853
AluBI AGCT 8 cut(s) 69, 231, 437, 760, 786, 914, 1017, 1056
AluI AGCT 8 cut(s) 69, 231, 437, 760, 786, 914, 1017, 1056
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 3 cut(s) 462, 533, 722
AlwNI CAGNNNCTG 2 cut(s) 138, 1020
Ama87I CYCGRG 1 cut(s) 64
ApeKI GCWGC 5 cut(s) 662, 1014, 1035, 1053, 1056
ApoI RAATTY 2 cut(s) 364, 733
ArsI GACNNNNNNTTYG 2 cut(s) 578, 610
AspA2I CCTAGG 1 cut(s) 671
AspS9I GGNCC 5 cut(s) 127, 418, 796, 884, 954
AsuC2I CCSGG 1 cut(s) 888
AsuHPI GGTGA 3 cut(s) 64, 716, 955
AvaI CYCGRG 1 cut(s) 64
AvaII GGWCC 5 cut(s) 127, 418, 796, 884, 954
AvrII CCTAGG 1 cut(s) 671
BaeGI GKGCMC 1 cut(s) 298
BanII GRGCYC 1 cut(s) 233
BbsI GAAGAC 1 cut(s) 202
Bbv12I GWGCWC 1 cut(s) 233
BbvI GCAGC 5 cut(s) 674, 1022, 1026, 1043, 1065
BccI CCATC 1 cut(s) 650
BceAI ACGGC 2 cut(s) 271, 486
BciT130I CCWGG 1 cut(s) 855
BcnI CCSGG 1 cut(s) 888
BcoDI GTCTC 3 cut(s) 462, 533, 722
BfaI CTAG 1 cut(s) 672
BfuAI ACCTGC 2 cut(s) 279, 556
BglII AGATCT 1 cut(s) 947
BisI GCNGC 5 cut(s) 663, 1015, 1036, 1054, 1057
BlnI CCTAGG 1 cut(s) 671
BlsI GCNGC 5 cut(s) 664, 1016, 1037, 1055, 1058
Bme1390I CCNGG 2 cut(s) 855, 888
Bme18I GGWCC 5 cut(s) 127, 418, 796, 884, 954
BmeT110I CYCGRG 1 cut(s) 64
BmgBI CACGTC 1 cut(s) 896
BmgT120I GGNCC 5 cut(s) 127, 418, 796, 884, 954
BmiI GGNNCC 3 cut(s) 886, 955, 998
BmrFI CCNGG 2 cut(s) 855, 888
BmsI GCATC 5 cut(s) 500, 589, 793, 802, 1035
BpiI GAAGAC 1 cut(s) 202
BpmI CTGGAG 1 cut(s) 13
BpuMI CCSGG 1 cut(s) 888
Bsa29I ATCGAT 1 cut(s) 576
BsaHI GRCGYC 1 cut(s) 190
BsaJI CCNNGG 3 cut(s) 442, 671, 853
BsaWI WCCGGW 1 cut(s) 920
BsaXI ACNNNNNCTCC 2 cut(s) 572, 602
Bsc4I CCNNNNNNNGG 1 cut(s) 720
Bse118I RCCGGY 1 cut(s) 815
BseBI CCWGG 1 cut(s) 855
BseCI ATCGAT 1 cut(s) 576
BseDI CCNNGG 3 cut(s) 442, 671, 853
BseGI GGATG 2 cut(s) 313, 340
BseLI CCNNNNNNNGG 1 cut(s) 720
BseMII CTCAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 5 cut(s) 674, 1022, 1026, 1043, 1065
Bsh1236I CGCG 2 cut(s) 95, 171
BshVI ATCGAT 1 cut(s) 576
BsiHKAI GWGCWC 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 64
BsiSI CCGG 3 cut(s) 816, 888, 921
BsiWI CGTACG 1 cut(s) 166
BslFI GGGAC 1 cut(s) 870
BslI CCNNNNNNNGG 1 cut(s) 720
BsmAI GTCTC 3 cut(s) 462, 533, 722
BsmFI GGGAC 1 cut(s) 870
BsmI GAATGC 2 cut(s) 698, 1049
BsoBI CYCGRG 1 cut(s) 64
Bsp1286I GDGCHC 2 cut(s) 233, 298
Bsp143I GATC 3 cut(s) 202, 931, 947
BspACI CCGC 2 cut(s) 95, 171
BspCNI CTCAG 1 cut(s) 36
BspDI ATCGAT 1 cut(s) 576
BspFNI CGCG 2 cut(s) 95, 171
BspLI GGNNCC 3 cut(s) 886, 955, 998
BspMI ACCTGC 2 cut(s) 279, 556
BsrFI RCCGGY 1 cut(s) 815
BssAI RCCGGY 1 cut(s) 815
BssECI CCNNGG 3 cut(s) 442, 671, 853
BssMI GATC 3 cut(s) 202, 931, 947
BssNAI GTATAC 1 cut(s) 449
BssNI GRCGYC 1 cut(s) 190
BssT1I CCWWGG 2 cut(s) 442, 671
Bst1107I GTATAC 1 cut(s) 449
Bst2UI CCWGG 1 cut(s) 855
Bst4CI ACNGT 4 cut(s) 85, 391, 397, 484
BstACI GRCGYC 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 812
BstDEI CTNAG 2 cut(s) 44, 756
BstENI CCTNNNNNAGG 1 cut(s) 718
BstF5I GGATG 2 cut(s) 313, 340
BstFNI CGCG 2 cut(s) 95, 171
BstKTI GATC 3 cut(s) 205, 934, 950
BstMAI GTCTC 3 cut(s) 462, 533, 722
BstMBI GATC 3 cut(s) 202, 931, 947
BstMWI GCNNNNNNNGC 5 cut(s) 812, 1023, 1032, 1044, 1053
BstNI CCWGG 1 cut(s) 855
BstSCI CCNGG 2 cut(s) 853, 886
BstSLI GKGCMC 1 cut(s) 298
BstUI CGCG 2 cut(s) 95, 171
BstV1I GCAGC 5 cut(s) 674, 1022, 1026, 1043, 1065
BstV2I GAAGAC 1 cut(s) 202
BstX2I RGATCY 1 cut(s) 947
BstYI RGATCY 1 cut(s) 947
BstZ17I GTATAC 1 cut(s) 449
Bsu15I ATCGAT 1 cut(s) 576
BsuTUI ATCGAT 1 cut(s) 576
BtrI CACGTC 1 cut(s) 896
BtsCI GGATG 2 cut(s) 313, 340
BtsIMutI CAGTG 2 cut(s) 90, 402
BveI ACCTGC 2 cut(s) 279, 556
CaiI CAGNNNCTG 2 cut(s) 138, 1020
Cfr10I RCCGGY 1 cut(s) 815
Cfr13I GGNCC 5 cut(s) 127, 418, 796, 884, 954
ClaI ATCGAT 1 cut(s) 576
Csp6I GTAC 4 cut(s) 160, 167, 277, 284
CviAII CATG 3 cut(s) 11, 653, 877
CviQI GTAC 4 cut(s) 160, 167, 277, 284
DdeI CTNAG 2 cut(s) 44, 756
DpnI GATC 3 cut(s) 204, 933, 949
DpnII GATC 3 cut(s) 202, 931, 947
Ecl136II GAGCTC 1 cut(s) 231
Eco130I CCWWGG 2 cut(s) 442, 671
Eco24I GRGCYC 1 cut(s) 233
Eco47I GGWCC 5 cut(s) 127, 418, 796, 884, 954
Eco53kI GAGCTC 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 231
EcoNI CCTNNNNNAGG 1 cut(s) 718
EcoO109I RGGNCCY 3 cut(s) 418, 884, 954
EcoRII CCWGG 1 cut(s) 853
EcoT14I CCWWGG 2 cut(s) 442, 671
EcoT22I ATGCAT 2 cut(s) 493, 808
EcoT38I GRGCYC 1 cut(s) 233
ErhI CCWWGG 2 cut(s) 442, 671
FaeI CATG 3 cut(s) 14, 656, 880
FaqI GGGAC 1 cut(s) 870
FatI CATG 3 cut(s) 10, 652, 876
FauNDI CATATG 1 cut(s) 808
FblI GTMKAC 1 cut(s) 448
Fnu4HI GCNGC 5 cut(s) 663, 1015, 1036, 1054, 1057
FokI GGATG 2 cut(s) 320, 347
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 5 cut(s) 663, 1015, 1036, 1054, 1057
FspBI CTAG 1 cut(s) 672
GluI GCNGC 5 cut(s) 663, 1015, 1036, 1054, 1057
GsuI CTGGAG 1 cut(s) 13
HapII CCGG 3 cut(s) 816, 888, 921
Hin1I GRCGYC 1 cut(s) 190
Hin1II CATG 3 cut(s) 14, 656, 880
HinfI GANTC 9 cut(s) 135, 179, 427, 521, 587, 683, 763, 777, 835
HpaII CCGG 3 cut(s) 816, 888, 921
HphI GGTGA 3 cut(s) 64, 716, 955
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 7 cut(s) 115, 221, 426, 498, 526, 688, 800
Hpy188III TCNNGA 8 cut(s) 30, 183, 194, 416, 557, 610, 774, 825
Hpy8I GTNNAC 1 cut(s) 449
HpyAV CCTTC 2 cut(s) 257, 431
HpyCH4III ACNGT 4 cut(s) 85, 391, 397, 484
HpyCH4IV ACGT 2 cut(s) 190, 895
HpyF10VI GCNNNNNNNGC 5 cut(s) 812, 1023, 1032, 1044, 1053
HpyF3I CTNAG 2 cut(s) 44, 756
HpySE526I ACGT 2 cut(s) 190, 895
Hsp92I GRCGYC 1 cut(s) 190
Hsp92II CATG 3 cut(s) 14, 656, 880
Kzo9I GATC 3 cut(s) 202, 931, 947
LmnI GCTCC 1 cut(s) 1002
Lsp1109I GCAGC 5 cut(s) 674, 1022, 1026, 1043, 1065
LweI GCATC 5 cut(s) 500, 589, 793, 802, 1035
MaeI CTAG 1 cut(s) 672
MaeII ACGT 2 cut(s) 190, 895
MaeIII GTNAC 2 cut(s) 391, 822
MalI GATC 3 cut(s) 204, 933, 949
MboI GATC 3 cut(s) 202, 931, 947
MboII GAAGA 6 cut(s) 29, 67, 202, 205, 1052, 1054
MfeI CAATTG 1 cut(s) 625
MflI RGATCY 1 cut(s) 947
MhlI GDGCHC 2 cut(s) 233, 298
MluCI AATT 8 cut(s) 38, 122, 224, 364, 508, 625, 639, 733
MlyI GAGTC 4 cut(s) 515, 581, 692, 757
MmeI TCCRAC 3 cut(s) 132, 285, 498
MnlI CCTC 7 cut(s) 37, 94, 179, 339, 714, 724, 884
Mph1103I ATGCAT 2 cut(s) 493, 808
MseI TTAA 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 1056
MspI CCGG 3 cut(s) 816, 888, 921
MspR9I CCNGG 2 cut(s) 855, 888
MunI CAATTG 1 cut(s) 625
Mva1269I GAATGC 2 cut(s) 698, 1049
MvaI CCWGG 1 cut(s) 855
MvnI CGCG 2 cut(s) 95, 171
MwoI GCNNNNNNNGC 5 cut(s) 812, 1023, 1032, 1044, 1053
NciI CCSGG 1 cut(s) 888
NdeI CATATG 1 cut(s) 808
NdeII GATC 3 cut(s) 202, 931, 947
NlaIII CATG 3 cut(s) 14, 656, 880
NlaIV GGNNCC 3 cut(s) 886, 955, 998
NmuCI GTSAC 1 cut(s) 822
NsiI ATGCAT 2 cut(s) 493, 808
PaeR7I CTCGAG 1 cut(s) 64
PaqCI CACCTGC 2 cut(s) 279, 556
PcsI WCGNNNNNNNCGW 3 cut(s) 111, 204, 213
PctI GAATGC 2 cut(s) 698, 1049
PfeI GAWTC 5 cut(s) 135, 179, 427, 777, 835
Pfl23II CGTACG 1 cut(s) 166
PfoI TCCNGGA 1 cut(s) 886
PkrI GCNGC 5 cut(s) 664, 1016, 1037, 1055, 1058
PleI GAGTC 4 cut(s) 515, 581, 691, 757
PpsI GAGTC 4 cut(s) 515, 581, 691, 757
PpuMI RGGWCCY 3 cut(s) 418, 884, 954
Psp124BI GAGCTC 1 cut(s) 233
Psp5II RGGWCCY 3 cut(s) 418, 884, 954
Psp6I CCWGG 1 cut(s) 853
PspGI CCWGG 1 cut(s) 853
PspLI CGTACG 1 cut(s) 166
PspN4I GGNNCC 3 cut(s) 886, 955, 998
PspPI GGNCC 5 cut(s) 127, 418, 796, 884, 954
PspPPI RGGWCCY 3 cut(s) 418, 884, 954
PspXI VCTCGAGB 1 cut(s) 64
PstNI CAGNNNCTG 2 cut(s) 138, 1020
PsuI RGATCY 1 cut(s) 947
PvuII CAGCTG 1 cut(s) 1056
RsaI GTAC 4 cut(s) 161, 168, 278, 285
RsaNI GTAC 4 cut(s) 160, 167, 277, 284
SacI GAGCTC 1 cut(s) 233
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 5 cut(s) 663, 1015, 1036, 1054, 1057
Sau3AI GATC 3 cut(s) 202, 931, 947
Sau96I GGNCC 5 cut(s) 127, 418, 796, 884, 954
SchI GAGTC 4 cut(s) 515, 581, 692, 757
ScrFI CCNGG 2 cut(s) 855, 888
SduI GDGCHC 2 cut(s) 233, 298
SfaNI GCATC 5 cut(s) 500, 589, 793, 802, 1035
Sfr274I CTCGAG 1 cut(s) 64
SinI GGWCC 5 cut(s) 127, 418, 796, 884, 954
SlaI CTCGAG 1 cut(s) 64
SmlI CTYRAG 1 cut(s) 64
SmoI CTYRAG 1 cut(s) 64
Sse9I AATT 8 cut(s) 38, 122, 224, 364, 508, 625, 639, 733
SsiI CCGC 2 cut(s) 95, 171
SspMI CTAG 1 cut(s) 672
SstI GAGCTC 1 cut(s) 233
StyD4I CCNGG 2 cut(s) 853, 886
StyI CCWWGG 2 cut(s) 442, 671
TaaI ACNGT 4 cut(s) 85, 391, 397, 484
TaiI ACGT 2 cut(s) 193, 898
TaqI TCGA 3 cut(s) 65, 120, 576
TasI AATT 8 cut(s) 38, 122, 224, 364, 508, 625, 639, 733
TatI WGTACW 1 cut(s) 159
TfiI GAWTC 5 cut(s) 135, 179, 427, 777, 835
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 2 cut(s) 90, 402
TseFI GTSAC 1 cut(s) 822
TseI GCWGC 5 cut(s) 662, 1014, 1035, 1053, 1056
Tsp45I GTSAC 1 cut(s) 822
TspDTI ATGAA 4 cut(s) 372, 705, 761, 893
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 2 cut(s) 90, 402
VpaK11BI GGWCC 5 cut(s) 127, 418, 796, 884, 954
XagI CCTNNNNNAGG 1 cut(s) 718
XapI RAATTY 2 cut(s) 364, 733
XcmI CCANNNNNNNNNTGG 1 cut(s) 947
XhoI CTCGAG 1 cut(s) 64
XmaJI CCTAGG 1 cut(s) 671
XmiI GTMKAC 1 cut(s) 448
XspI CTAG 1 cut(s) 672
ZraI GACGTC 1 cut(s) 191
Zsp2I ATGCAT 2 cut(s) 493, 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.