Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0005524

Physical Location & Seq

Genomic Coordinates Forward (+)

25540869 .. 25545094

Gene Structure 4226 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g34350.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2655 bp

ATGTCTTGGGGTCTCTTGGTTTTGCTGCTGTTGGTGAGTTCATCCCTGGCCAATGGAAGAGGGGATAACACCCAACAACACAGAGTTGCAACAATAGGAGCTGTAGTTGATTTCACTTCTCGTGTGGGAAAAGAACAGAGAATAGCCATGGATTTGGCTATCCAAGACCTTCTTCACTCGAACTGCCTTACAGTAAACTTAGACCTCAGAGATTCACAAGGCAATTCTGCTAAATCAACTTCTGCTGCAATCAATCTTCTGAATGATAAAAAAGTTGAAGTCATCATTGGAACTCTAACCATGCAAGAAGCAGCTGTAGTTTCTGAGATCGAAATTCGGAAGGCAAACTTCACCATTTTATCACTTACTTCAACTGCCATAAAACCATCCCCATCCCCAGCCTCACTTCAACTGCCAAATTATCTCCAAGTAGCAAATGACATTGTTATCTATATGCAATGTTTAGCAGCCTTAGTTGGCCATTTTCAATGGAGAAAGGTGACTGCACTTTATGAACAAAACATTGACTTTTCCTCCAATTCTGGAATCATAACCCTACTCTCTGATTCACTGAAATTGGTCAACTCAGAGATTGAACACCACCAAGCTTTCCCAACCCTCTCTTCTCTCTCTGATCCAAATGCCTTGTTCGATGAAGAGCTAAAGAAGTTGAGGAGCAAGAGAAACAGAGTTTTCATAGTCACCCAATTCTCTTTGAATTCAGCTGTTTTTCTCTTTCGGAAGGCAAAGGAAATGGGGATGATGGGTAAAGGCTATGTATGGATTGTCACAGATGAGATTGCAAGTCTACTTGACTCTGTTGATGACTCTGTCAAGTACAATATGCAAGGTGTTATTGGCATTAAAACCAACTTCATAGAATCAACCAAAAATTTCGCGAAGTTCAAAACCAGATTTCGCAGACTGTATGGACTGCAATACCCTGAAGAAGAAGAGAACTCTAGTCCAAGCATCTTTGCTCTTCGAACATATGACACAATGCACGCCATTGCTCTGGCCATGAAAAATGCTGCCTCAAAATATTTGTCTCAAGAAATCGTGTCCACCGAATTCCAAGGTCTAAGTGGCATGATGCAGTTCAAGAATGGCATGCTATCCCAATCACCAACCTTTCAAATCATAAATGTGTTTGGCAGAAGCTACAGCGAGATTGCATTCTGGTCACCGCGCTTTGGTTTCTCAGATAGCATGAACAAACACCTTGATATGAAGGAGAGGACTAATCACAGTTATGTTCAAGTTTTGGGTCCAATCAACTGGCCAGGTGGCTTACAAGCACCCCCAAAGGGGTGGACTCTTGTCGATGAAGAAAGACCCTTGAAGATAGGGATTCCAGCGAGGGGTGCCTTCAATCAGTTTGTGAAAGTAACATATGACCAGAACACGAATCAAACACGCATCACAGGGTTTTCAATTGATATGTTTGAAGCAGCTGTCAAAAACCTGCCTTATCATATGCCTTATGTGTTTGTTCCCTTCAATGGCTCATATGATGAATTGGTGGAACAGGTCTACTACAAGGGCTTGGATGCAGCTGTTGGTGATATAGAAGTCATAGCTTACCGGTATCATTATGTTGAGTTCTCACAACCATATATAAGCTCTGGACTTGAGATGATAGTCCCTGTAAAGTCAGATAAGCTGAAGGAAAAATGGATGTTCATGAAGGCCTTCAGCAAAACCATGTGGTTTCTCATGATCATTATACATCTATCTGTATGCTTTGTCGTCTGTTTTATTGAAAATGAGCATGGCCAAAACTTTGAATTAAAGAGAATTGGGGACATCCTGTGGTTCTCTGTCACCGTCTTATTTTTCGCGCAAAGAGAACGAGTCCAGAGTAATTTGGCAAGGCTGGTATTGGCTCCATGGTTACTAGTGATTCTACTAGTAGTGGCCACTTTCACAGCGAGCCTCTCTTCCATGATGACTATCTCTCGAGTCCAGCCATCTGTGCTCGATGTGGAAACACTGAAGAAAGGAAATGGAACTGTTGGATGTAACGGCAACTCCTTTATCGTAAGGTACCTTATCGATGTTCTAGAGTTCAGACCAGAAAACATCAAGAGAATTTCCGCCATAAGTGACTACCCAAAGGCCTTTGAGAGTGGAGAAATTCAAGCAGCTTTCTTCGTTGCGCCCCATGCTAAAGTCTTCCTCGCATGTTACTGCAAGGGCTATATGAAAGCAGGTCCGATATACAAGCCTAGCGGCTTTGCATTTGTTTTTCCAAAGGGGTCTCCTCTGGCCATTGACATGTCAAAGGCAATCGTAGAAGTAAACGAGAAGAAGCAAGTGGAGCTATTAGAAAGACAAATGCTCTCCTCCTTCAACTGTTCCCCTTCACTCAACTCGAACGATGGTCCAATAGGTTATGAGCCCTTTTCTGGCCTATTTCTAATCTCAGGTTTGGTTTGTGCATTTGGGCTTCTGGTTACGAGCGGGCGTAAAGCCGATAGGCTTCTACACAATATGAGCTGCATACGAGCAATACTAATCAAAACAAGAACTCTGACATGGGTTTTTCTGTACTTGAATCAGAGCTACAGACAATCTCAAAAGCATTGCTCCACAAGCTCCATTCTAGAAACTTGCTCTACAAGGAACATGAGTAGTGGTGTTATAGTGATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

885

Amino Acids

99.18

Weight (kDa)

8.78

Isoelectric Point (pI)

38.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	31 - 352	1.3e-08	Periplasmic binding protein
ANF_receptor	PF01094	48 - 383	5.8e-40	Receptor family ligand binding region
SBP_bac_3	PF00497	469 - 776	2e-08	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	567 - 811	1.4e-19	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005524)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g34350 FvH4_2g34350 FvH4_2g34350 FvH4_2g34350 FvH4_2g34350 FvH4_2g34370
malus_domestica	MD15G1082100.v1.1
prunus_persica	Prupe.1G434800_v2.0.a1
pyrus_communis	pycom15g07690
rosa_chinensis	RchiOBHm_Chr6g0308001 RchiOBHm_Chr6g0308011
rosa_laevigata	RLG00000010676 RLG00000010677 RLG00000010678 RLG00000010679
rosa_multiflora	Rmu_sc0006237.1_g000009 Rmu_sc0006237.1_g000010
rosa_roxburghii	Rroxscaffold_7G00160680 Rroxscaffold_7G00160690
rosa_rugosa	Rorug06G0362900 Rorug06G0363000
rosa_samantha	Rh6BG482500 Rh6BG482700 Rh6CG487800 Rh6CG488000 Rh6DG474100 Rh6DG474200
rosa_wichuraiana	Rw6G041170 Rw6G041180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1473, 2201
Acc65I	GGTACC	1 cut(s)	2046
AccB1I	GGYRCC	2 cut(s)	1364, 2046
AccBSI	CCGCTC	1 cut(s)	2463
AccI	GTMKAC	2 cut(s)	808, 1533
AccII	CGCG	3 cut(s)	899, 1189, 1841
AciI	CCGC	4 cut(s)	1187, 2097, 2232, 2463
AclWI	GGATC	1 cut(s)	629
AcoI	YGGCCR	7 cut(s)	48, 478, 1017, 1280, 1774, 1917, 2268
AcsI	RAATTY	6 cut(s)	333, 718, 892, 1070, 2091, 2136
AcuI	CTGAAG	4 cut(s)	966, 1678, 1685, 2015
AfaI	GTAC	3 cut(s)	839, 2048, 2552
AfiI	CCNNNNNNNGG	6 cut(s)	1193, 1307, 1308, 1361, 1502, 2408
AflIII	ACRYGT	1 cut(s)	2277
AgeI	ACCGGT	1 cut(s)	1584
AhlI	ACTAGT	2 cut(s)	1897, 1909
AjnI	CCWGG	2 cut(s)	45, 1282
AjuI	GAANNNNNNNTTGG	6 cut(s)	156, 188, 270, 302, 607, 639
Alw21I	GWGCWC	1 cut(s)	1980
Alw26I	GTCTC	3 cut(s)	17, 1053, 2265
AlwI	GGATC	1 cut(s)	629
Ama87I	CYCGRG	1 cut(s)	1959
ApeKI	GCWGC	9 cut(s)	25, 245, 311, 467, 1031, 1451, 1553, 2144, 2499
ApoI	RAATTY	6 cut(s)	333, 718, 892, 1070, 2091, 2136
ArsI	GACNNNNNNTTYG	2 cut(s)	1031, 1063
AsiGI	ACCGGT	1 cut(s)	1584
Asp700I	GAANNNNTTC	1 cut(s)	1691
Asp718I	GGTACC	1 cut(s)	2046
AspLEI	GCGC	3 cut(s)	1191, 1843, 2161
AspS9I	GGNCC	3 cut(s)	1268, 2213, 2384
AsuHPI	GGTGA	8 cut(s)	46, 343, 511, 694, 1116, 1176, 1574, 1816
AsuII	TTCGAA	1 cut(s)	985
AvaI	CYCGRG	1 cut(s)	1959
AvaII	GGWCC	3 cut(s)	1268, 2213, 2384
BalI	TGGCCA	7 cut(s)	50, 480, 1019, 1282, 1776, 1919, 2270
BanI	GGYRCC	2 cut(s)	1364, 2046
BanII	GRGCYC	1 cut(s)	2403
BauI	CACGAG	1 cut(s)	120
BbsI	GAAGAC	1 cut(s)	2167
Bbv12I	GWGCWC	1 cut(s)	1980
BbvI	GCAGC	9 cut(s)	12, 232, 323, 479, 1018, 1463, 1565, 2156, 2486
BccI	CCATC	5 cut(s)	394, 400, 757, 1978, 2375
BceAI	ACGGC	1 cut(s)	2041
BciT130I	CCWGG	2 cut(s)	47, 1284
BclI	TGATCA	1 cut(s)	1719
BcoDI	GTCTC	3 cut(s)	17, 1053, 2265
BcuI	ACTAGT	2 cut(s)	1897, 1909
BfaI	CTAG	6 cut(s)	963, 1898, 1910, 2063, 2229, 2606
BfmI	CTRYAG	4 cut(s)	102, 315, 1162, 2566
BfuAI	ACCTGC	2 cut(s)	1473, 2201
Bme1390I	CCNGG	2 cut(s)	47, 1284
Bme18I	GGWCC	3 cut(s)	1268, 2213, 2384
BmeT110I	CYCGRG	1 cut(s)	1959
BmgT120I	GGNCC	3 cut(s)	1268, 2213, 2384
BmiI	GGNNCC	4 cut(s)	1269, 1366, 1887, 2048
BmrFI	CCNGG	2 cut(s)	47, 1284
BmsI	GCATC	4 cut(s)	981, 1083, 1428, 1540
BoxI	GACNNNNGTC	1 cut(s)	1319
BpiI	GAAGAC	1 cut(s)	2167
Bpu14I	TTCGAA	1 cut(s)	985
BpuEI	CTTGAG	2 cut(s)	1035, 1652
Bsa29I	ATCGAT	1 cut(s)	2055
BsaBI	GATNNNNATC	1 cut(s)	1952
BsaI	GGTCTC	2 cut(s)	17, 2265
BsaJI	CCNNGG	4 cut(s)	45, 147, 1075, 1889
BsaWI	WCCGGW	1 cut(s)	1584
BsaXI	ACNNNNNCTCC	6 cut(s)	90, 120, 518, 548, 2015, 2045
Bsc4I	CCNNNNNNNGG	6 cut(s)	1193, 1307, 1308, 1361, 1502, 2408
Bse118I	RCCGGY	1 cut(s)	1584
Bse1I	ACTGG	1 cut(s)	1283
Bse3DI	GCAATG	3 cut(s)	464, 1008, 2584
Bse8I	GATNNNNATC	1 cut(s)	1952
BseBI	CCWGG	2 cut(s)	47, 1284
BseCI	ATCGAT	1 cut(s)	2055
BseDI	CCNNGG	4 cut(s)	45, 147, 1075, 1889
BseGI	GGATG	8 cut(s)	41, 386, 392, 765, 1555, 1683, 1806, 2024
BseJI	GATNNNNATC	1 cut(s)	1952
BseLI	CCNNNNNNNGG	6 cut(s)	1193, 1307, 1308, 1361, 1502, 2408
BseMI	GCAATG	3 cut(s)	464, 1008, 2584
BseMII	CTCAG	5 cut(s)	220, 315, 600, 1215, 2439
BseNI	ACTGG	1 cut(s)	1283
BseRI	GAGGAG	3 cut(s)	688, 2253, 2335
BseXI	GCAGC	9 cut(s)	12, 232, 323, 479, 1018, 1463, 1565, 2156, 2486
BseYI	CCCAGC	1 cut(s)	397
BsgI	GTGCAG	1 cut(s)	489
Bsh1236I	CGCG	3 cut(s)	899, 1189, 1841
BshNI	GGYRCC	2 cut(s)	1364, 2046
BshTI	ACCGGT	1 cut(s)	1584
BshVI	ATCGAT	1 cut(s)	2055
BsiHKAI	GWGCWC	1 cut(s)	1980
BsiHKCI	CYCGRG	1 cut(s)	1959
BsiSI	CCGG	1 cut(s)	1585
BslFI	GGGAC	2 cut(s)	1628, 1817
BslI	CCNNNNNNNGG	6 cut(s)	1193, 1307, 1308, 1361, 1502, 2408
BsmAI	GTCTC	3 cut(s)	17, 1053, 2265
BsmFI	GGGAC	2 cut(s)	1628, 1817
BsmI	GAATGC	1 cut(s)	1175
Bso31I	GGTCTC	2 cut(s)	17, 2265
BsoBI	CYCGRG	1 cut(s)	1959
Bsp119I	TTCGAA	1 cut(s)	985
Bsp1286I	GDGCHC	2 cut(s)	1980, 2403
Bsp143I	GATC	3 cut(s)	327, 634, 1719
Bsp19I	CCATGG	2 cut(s)	147, 1889
Bsp68I	TCGCGA	1 cut(s)	899
BspACI	CCGC	4 cut(s)	1187, 2097, 2232, 2463
BspCNI	CTCAG	5 cut(s)	219, 316, 599, 1214, 2438
BspDI	ATCGAT	1 cut(s)	2055
BspFNI	CGCG	3 cut(s)	899, 1189, 1841
BspHI	TCATGA	2 cut(s)	1683, 1716
BspLI	GGNNCC	4 cut(s)	1269, 1366, 1887, 2048
BspMI	ACCTGC	2 cut(s)	1473, 2201
BspPI	GGATC	1 cut(s)	629
BspQI	GCTCTTC	2 cut(s)	651, 987
BspT104I	TTCGAA	1 cut(s)	985
BspT107I	GGYRCC	2 cut(s)	1364, 2046
BspTNI	GGTCTC	2 cut(s)	17, 2265
BsrBI	CCGCTC	1 cut(s)	2463
BsrDI	GCAATG	3 cut(s)	464, 1008, 2584
BsrFI	RCCGGY	1 cut(s)	1584
BsrI	ACTGG	1 cut(s)	1283
BssAI	RCCGGY	1 cut(s)	1584
BssECI	CCNNGG	4 cut(s)	45, 147, 1075, 1889
BssMI	GATC	3 cut(s)	327, 634, 1719
BssSI	CACGAG	1 cut(s)	120
BssT1I	CCWWGG	3 cut(s)	147, 1075, 1889
Bst2BI	CACGAG	1 cut(s)	120
Bst2UI	CCWGG	2 cut(s)	47, 1284
Bst4CI	ACNGT	6 cut(s)	193, 927, 1250, 1828, 2014, 2357
Bst6I	CTCTTC	6 cut(s)	52, 628, 651, 948, 987, 1945
BstBI	TTCGAA	1 cut(s)	985
BstC8I	GCNNGC	4 cut(s)	1005, 1112, 1933, 2465
BstDEI	CTNAG	8 cut(s)	199, 206, 324, 472, 586, 1082, 1201, 2425
BstDSI	CCRYGG	2 cut(s)	147, 1889
BstEII	GGTNACC	1 cut(s)	1182
BstF5I	GGATG	8 cut(s)	41, 386, 392, 765, 1555, 1683, 1806, 2024
BstFNI	CGCG	3 cut(s)	899, 1189, 1841
BstHHI	GCGC	3 cut(s)	1191, 1843, 2161
BstKTI	GATC	3 cut(s)	330, 637, 1722
BstMAI	GTCTC	3 cut(s)	17, 1053, 2265
BstMBI	GATC	3 cut(s)	327, 634, 1719
BstMWI	GCNNNNNNNGC	5 cut(s)	1364, 1975, 2165, 2320, 2595
BstNI	CCWGG	2 cut(s)	47, 1284
BstNSI	RCATGY	3 cut(s)	1114, 2187, 2281
BstPAI	GACNNNNGTC	1 cut(s)	1319
BstPI	GGTNACC	1 cut(s)	1182
BstSCI	CCNGG	2 cut(s)	45, 1282
BstSFI	CTRYAG	4 cut(s)	102, 315, 1162, 2566
BstUI	CGCG	3 cut(s)	899, 1189, 1841
BstV1I	GCAGC	9 cut(s)	12, 232, 323, 479, 1018, 1463, 1565, 2156, 2486
BstV2I	GAAGAC	1 cut(s)	2167
BstXI	CCANNNNNNTGG	4 cut(s)	154, 1015, 1278, 1311
Bsu15I	ATCGAT	1 cut(s)	2055
BsuTUI	ATCGAT	1 cut(s)	2055
BtgI	CCRYGG	2 cut(s)	147, 1889
BtsCI	GGATG	8 cut(s)	41, 386, 392, 765, 1555, 1683, 1806, 2024
BtsIMutI	CAGTG	2 cut(s)	569, 1991
BtuMI	TCGCGA	1 cut(s)	899
BveI	ACCTGC	2 cut(s)	1473, 2201
Cac8I	GCNNGC	4 cut(s)	1005, 1112, 1933, 2465
CciI	TCATGA	2 cut(s)	1683, 1716
CfoI	GCGC	3 cut(s)	1191, 1843, 2161
Cfr10I	RCCGGY	1 cut(s)	1584
Cfr13I	GGNCC	3 cut(s)	1268, 2213, 2384
ClaI	ATCGAT	1 cut(s)	2055
Csp6I	GTAC	3 cut(s)	838, 2047, 2551
CspAI	ACCGGT	1 cut(s)	1584
CviQI	GTAC	3 cut(s)	838, 2047, 2551
DdeI	CTNAG	8 cut(s)	199, 206, 324, 472, 586, 1082, 1201, 2425
DpnI	GATC	3 cut(s)	329, 636, 1721
DpnII	GATC	3 cut(s)	327, 634, 1719
EaeI	YGGCCR	7 cut(s)	48, 478, 1017, 1280, 1774, 1917, 2268
Eam1104I	CTCTTC	6 cut(s)	52, 628, 651, 948, 987, 1945
EarI	CTCTTC	6 cut(s)	52, 628, 651, 948, 987, 1945
EciI	GGCGGA	1 cut(s)	2086
Eco130I	CCWWGG	3 cut(s)	147, 1075, 1889
Eco147I	AGGCCT	2 cut(s)	1691, 2120
Eco24I	GRGCYC	1 cut(s)	2403
Eco31I	GGTCTC	2 cut(s)	17, 2265
Eco47I	GGWCC	3 cut(s)	1268, 2213, 2384
Eco57I	CTGAAG	4 cut(s)	966, 1678, 1685, 2015
Eco88I	CYCGRG	1 cut(s)	1959
Eco91I	GGTNACC	1 cut(s)	1182
EcoO65I	GGTNACC	1 cut(s)	1182
EcoRI	GAATTC	2 cut(s)	718, 1070
EcoRII	CCWGG	2 cut(s)	45, 1282
EcoT14I	CCWWGG	3 cut(s)	147, 1075, 1889
EcoT38I	GRGCYC	1 cut(s)	2403
ErhI	CCWWGG	3 cut(s)	147, 1075, 1889
FalI	AAGNNNNNCTT	4 cut(s)	156, 188, 332, 364
FaqI	GGGAC	2 cut(s)	1628, 1817
FauI	CCCGC	1 cut(s)	2456
FauNDI	CATATG	4 cut(s)	991, 1393, 1476, 1510
FbaI	TGATCA	1 cut(s)	1719
FblI	GTMKAC	2 cut(s)	808, 1533
FokI	GGATG	8 cut(s)	28, 373, 379, 772, 1562, 1690, 1793, 2031
FriOI	GRGCYC	1 cut(s)	2403
FspBI	CTAG	6 cut(s)	963, 1898, 1910, 2063, 2229, 2606
GlaI	GCGC	3 cut(s)	1190, 1842, 2160
GsaI	CCCAGC	1 cut(s)	401
HapII	CCGG	1 cut(s)	1585
HhaI	GCGC	3 cut(s)	1191, 1843, 2161
Hin6I	GCGC	3 cut(s)	1189, 1841, 2159
HinP1I	GCGC	3 cut(s)	1189, 1841, 2159
HincII	GTYRAC	1 cut(s)	583
HindII	GTYRAC	1 cut(s)	583
HindIII	AAGCTT	1 cut(s)	606
HpaII	CCGG	1 cut(s)	1585
HphI	GGTGA	8 cut(s)	46, 343, 511, 694, 1116, 1176, 1574, 1816
Hpy166II	GTNNAC	7 cut(s)	196, 583, 809, 1065, 1314, 1534, 2302
Hpy8I	GTNNAC	7 cut(s)	196, 583, 809, 1065, 1314, 1534, 2302
HpyCH4III	ACNGT	6 cut(s)	193, 927, 1250, 1828, 2014, 2357
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1364, 1975, 2165, 2320, 2595
HpyF3I	CTNAG	8 cut(s)	199, 206, 324, 472, 586, 1082, 1201, 2425
HspAI	GCGC	3 cut(s)	1189, 1841, 2159
KpnI	GGTACC	1 cut(s)	2050
Ksp22I	TGATCA	1 cut(s)	1719
Kzo9I	GATC	3 cut(s)	327, 634, 1719
LguI	GCTCTTC	2 cut(s)	651, 987
LmnI	GCTCC	6 cut(s)	98, 675, 1891, 2320, 2594, 2603
Lsp1109I	GCAGC	9 cut(s)	12, 232, 323, 479, 1018, 1463, 1565, 2156, 2486
LweI	GCATC	4 cut(s)	981, 1083, 1428, 1540
MaeI	CTAG	6 cut(s)	963, 1898, 1910, 2063, 2229, 2606
MalI	GATC	3 cut(s)	329, 636, 1721
MbiI	CCGCTC	1 cut(s)	2463
MboI	GATC	3 cut(s)	327, 634, 1719
MfeI	CAATTG	1 cut(s)	1434
MhlI	GDGCHC	2 cut(s)	1980, 2403
MlsI	TGGCCA	7 cut(s)	50, 480, 1019, 1282, 1776, 1919, 2270
MluNI	TGGCCA	7 cut(s)	50, 480, 1019, 1282, 1776, 1919, 2270
MlyI	GAGTC	5 cut(s)	809, 821, 1309, 1863, 1971
MmeI	TCCRAC	1 cut(s)	1996
Mox20I	TGGCCA	7 cut(s)	50, 480, 1019, 1282, 1776, 1919, 2270
MroXI	GAANNNNTTC	1 cut(s)	1691
MscI	TGGCCA	7 cut(s)	50, 480, 1019, 1282, 1776, 1919, 2270
MseI	TTAA	2 cut(s)	864, 1790
MslI	CAYNNNNRTG	3 cut(s)	1145, 1251, 2276
Msp20I	TGGCCA	7 cut(s)	50, 480, 1019, 1282, 1776, 1919, 2270
MspA1I	CMGCKG	4 cut(s)	314, 725, 1454, 1556
MspI	CCGG	1 cut(s)	1585
MspR9I	CCNGG	2 cut(s)	47, 1284
MunI	CAATTG	1 cut(s)	1434
Mva1269I	GAATGC	1 cut(s)	1175
MvaI	CCWGG	2 cut(s)	47, 1284
MvnI	CGCG	3 cut(s)	899, 1189, 1841
MwoI	GCNNNNNNNGC	5 cut(s)	1364, 1975, 2165, 2320, 2595
NcoI	CCATGG	2 cut(s)	147, 1889
NdeI	CATATG	4 cut(s)	991, 1393, 1476, 1510
NdeII	GATC	3 cut(s)	327, 634, 1719
NlaIV	GGNNCC	4 cut(s)	1269, 1366, 1887, 2048
NmuCI	GTSAC	6 cut(s)	499, 700, 787, 1182, 1822, 2105
NruI	TCGCGA	1 cut(s)	899
NspI	RCATGY	3 cut(s)	1114, 2187, 2281
NspV	TTCGAA	1 cut(s)	985
PaeI	GCATGC	1 cut(s)	1114
PaeR7I	CTCGAG	1 cut(s)	1959
PagI	TCATGA	2 cut(s)	1683, 1716
PceI	AGGCCT	2 cut(s)	1691, 2120
PciI	ACATGT	1 cut(s)	2277
PciSI	GCTCTTC	2 cut(s)	651, 987
PcsI	WCGNNNNNNNCGW	1 cut(s)	1065
PctI	GAATGC	1 cut(s)	1175
PdmI	GAANNNNTTC	1 cut(s)	1691
PfeI	GAWTC	8 cut(s)	212, 546, 566, 881, 1351, 1408, 1903, 2557
PflFI	GACNNNGTC	1 cut(s)	830
PinAI	ACCGGT	1 cut(s)	1584
PleI	GAGTC	5 cut(s)	809, 821, 1309, 1862, 1970
PpsI	GAGTC	5 cut(s)	809, 821, 1309, 1862, 1970
PscI	ACATGT	1 cut(s)	2277
PshAI	GACNNNNGTC	1 cut(s)	1319
Psp6I	CCWGG	2 cut(s)	45, 1282
PspEI	GGTNACC	1 cut(s)	1182
PspFI	CCCAGC	1 cut(s)	397
PspGI	CCWGG	2 cut(s)	45, 1282
PspN4I	GGNNCC	4 cut(s)	1269, 1366, 1887, 2048
PspPI	GGNCC	3 cut(s)	1268, 2213, 2384
PsyI	GACNNNGTC	1 cut(s)	830
PvuII	CAGCTG	4 cut(s)	314, 725, 1454, 1556
RruI	TCGCGA	1 cut(s)	899
RsaI	GTAC	3 cut(s)	839, 2048, 2552
RsaNI	GTAC	3 cut(s)	838, 2047, 2551
RseI	CAYNNNNRTG	3 cut(s)	1145, 1251, 2276
SapI	GCTCTTC	2 cut(s)	651, 987
SaqAI	TTAA	2 cut(s)	864, 1790
Sau3AI	GATC	3 cut(s)	327, 634, 1719
Sau96I	GGNCC	3 cut(s)	1268, 2213, 2384
SchI	GAGTC	5 cut(s)	809, 821, 1309, 1863, 1971
ScrFI	CCNGG	2 cut(s)	47, 1284
SduI	GDGCHC	2 cut(s)	1980, 2403
SfaNI	GCATC	4 cut(s)	981, 1083, 1428, 1540
SfcI	CTRYAG	4 cut(s)	102, 315, 1162, 2566
Sfr274I	CTCGAG	1 cut(s)	1959
SfuI	TTCGAA	1 cut(s)	985
SinI	GGWCC	3 cut(s)	1268, 2213, 2384
SlaI	CTCGAG	1 cut(s)	1959
SmiMI	CAYNNNNRTG	3 cut(s)	1145, 1251, 2276
SmlI	CTYRAG	3 cut(s)	1050, 1631, 1959
SmoI	CTYRAG	3 cut(s)	1050, 1631, 1959
SpeI	ACTAGT	2 cut(s)	1897, 1909
SphI	GCATGC	1 cut(s)	1114
SseBI	AGGCCT	2 cut(s)	1691, 2120
SsiI	CCGC	4 cut(s)	1187, 2097, 2232, 2463
SspI	AATATT	1 cut(s)	1043
SspMI	CTAG	6 cut(s)	963, 1898, 1910, 2063, 2229, 2606
StuI	AGGCCT	2 cut(s)	1691, 2120
StyD4I	CCNGG	2 cut(s)	45, 1282
StyI	CCWWGG	3 cut(s)	147, 1075, 1889
TaaI	ACNGT	6 cut(s)	193, 927, 1250, 1828, 2014, 2357
TaqI	TCGA	9 cut(s)	179, 330, 651, 985, 1323, 1960, 1980, 2055, 2375
TatI	WGTACW	2 cut(s)	837, 2550
TauI	GCSGC	1 cut(s)	2235
TfiI	GAWTC	8 cut(s)	212, 546, 566, 881, 1351, 1408, 1903, 2557
Tru1I	TTAA	2 cut(s)	864, 1790
Tru9I	TTAA	2 cut(s)	864, 1790
TscAI	CASTG	2 cut(s)	576, 1998
TseFI	GTSAC	6 cut(s)	499, 700, 787, 1182, 1822, 2105
TseI	GCWGC	9 cut(s)	25, 245, 311, 467, 1031, 1451, 1553, 2144, 2499
Tsp45I	GTSAC	6 cut(s)	499, 700, 787, 1182, 1822, 2105
TspRI	CASTG	2 cut(s)	576, 1998
Tth111I	GACNNNGTC	1 cut(s)	830
VpaK11BI	GGWCC	3 cut(s)	1268, 2213, 2384
XapI	RAATTY	6 cut(s)	333, 718, 892, 1070, 2091, 2136
XbaI	TCTAGA	2 cut(s)	2062, 2605
XceI	RCATGY	3 cut(s)	1114, 2187, 2281
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1082
XhoI	CTCGAG	1 cut(s)	1959
XmiI	GTMKAC	2 cut(s)	808, 1533
XmnI	GAANNNNTTC	1 cut(s)	1691
XspI	CTAG	6 cut(s)	963, 1898, 1910, 2063, 2229, 2606

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.