Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0005524

Physical Location & Seq

Genomic Coordinates Forward (+)

5628730 .. 5632017

Gene Structure 3288 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1082100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2583 bp

ATGCTTCGTGGTCTTTCCTTCTTTCTGGTATTGCTATCGTTTGTGAGTTCAGCCAAGGCTAATGGAAGAGAGGAAAAATGTCAAGTAACTCAACAGATAACTGCAAGAATCGGTTGTGTCATCGATCTCAGCTCTCGCGTGGGTAAAGAACAAAAAATAGCCATGGAAATGGCTGTCCAAGATCTTTTTAAGTCGACCTGCGCTAAAGTGGACTTGCACCTGAAAGATTCTCAAGGGAACTCAGCTCGAGCAACAGCTGCAGCAATCAATCTCCTCAGCAGCAAACAGGTTGTGGCCATTGTCGGAGGACTGACCGTGCAGGAAGCAGCTCTTATCTCTGAGATTGATAATATCACCGAGAGTATTGTTCCCATCATATCCCTTACTTCCACTGCAACGATCCCGCGATCAGCGTCACTTCAAAGGCCAATTTTCATCCAAATAGCTAACGATATCGTCTTTCACATGCAATGCGTTGCAGCCTTAGTTGGTCATTTCCAATGGCGAAAGGTGACTGCAGTTTACGAGCGCAACAATGAATTTTCTTCCACTTCAGGGATCATAGCTCTCCTCTCTCATTCACTTAGCTTGGTCAACTCAGAGATTGAACACCACCGAGATTTCCCATCCTTGTCTTCTCTCTCTAACCCGGATGCCTTCGTTGAAGAAGAGCTAAAGAAGTTGAGAAGCAAGAACAATAGGGTCTTCATAGTCATGCAATTCTCTTTGCAGTCAGCTGTCGTTTTCTTCGAAAAAGTGAAGCAGATGGGCATGATGGACAAAGGCTATGCGTGGATTGTCACAGATGAGATTGCAAGCCTTCTCGATTCTGTTAACACTTCTGTCAAGTATAATATGCAAGGTGTTATTGGTATTAAAACCAATTTTTTCGAGACAACTGCGTCATTCAGACAGTTCAAAACCAGATTTCGCAGGATATACGGACTACAGTACCCTGACGAAGAAGAAACTTCTAGTCCTAGTATCTTCGCTCTCCGAGCTTATGATGCAACTGGGGCCATTACTCGAGCAGTGACAACATTGCAACAGGGAAATGCTACCTCAGAAGAATTGTCTCAAAAAATTTTGTCTACTGCTTCCCTAGGTCTCAGTGGCATGCTAGAATTCAAGAACGGTATGCTAGCACAACCACCGACGTTTCAAATCATTAACGTGGTTGGGAGAAGCTACAGAGAGATGGCATTTTGGTCGCCAAGATACGGTTTCTCAAAGAGCTTGATGGAAAAGGGGATGGATAATAGTTTTTCTGAAGTTTTGGGTTCAACCTATTGGCCTGGTGGCTTACAAACAACCCCAAATTGGTGGACTCCGGTCAATGTGGATCGACCCTTAAAGATAGGGGTTCCTGCCAGGGGTGCATTCAAGCAGTTTGTGGACGTAAAACATGATCCTGGCACGAATGAAACACGCATCTCGGGATTTTCAATCGATGTGTTTAAAGCAGCTGTCGAATATCTTCCTTATCAGTTGCCTTACGAGCTAGTTCCCTTCAATGGCTCGTATGATGAAATGGTGAAACTGGTCTACAATAAGAGCCTTGATGCAGCTGTTGGTGATTTCGAAGTCATATCTGATCGGTATCATTATGTTGAATTCACACAGCCATACATAAGCTCGGGGCTTGAGATGGTTGTTCCAGTGAAGCCAGATAAGCTGAAGGAAAAATGGATGTTCATGCGTGCCTTCACCACGGAAATGTGGTTCATGTTGTTTGTTACACACCTTGCTTTATGCTTCGTTGTCTGGTTAATTGAAAATGAACATGGAGACAACCCCGAAATAAAGGGAATTGGGGCAATGCTTTGGTTCTCTGTCACCATCCTCTTCTTTGCAAACAGAGAACAAGTTCATAACAATTGGGCTCGGTTGGTTCTGGCTCCATGGTTAGTTGTGATTCTAGCTGTAACGGCCACTTTCACTGCAAGCCTCACCTCCATGATGACTCTCTCTCAAGTCCACCCGTCCATGCTTGATATTGGCACACTTCATAAACTGAATGCAACCGTTGGTTGTAATGGCAACTCATTTATTGTTAGATATTTGACCGATGTTTTAGATTTTAGACCCGAAAACATCAAAAGTATTGCCTCCATAGGTGACTACCCAAATGCCTTTGAGAAGCAAGATATTGCTGCGGCTTTCTTTGTTGTGCCTCATGCTAAAGTCTTCCTCGCAAAATACTGCAAGGGCTACGTGACAACAGGGCCTGTTTACAAGCCTAGCGGTTTTGGATTTGTTTTTCCAAAGGGTTCTCCTCTGGCTATTGACATCTCGGAGGCAATGCTAGAGGCAACTGAGAATGGACAAATCGAAAAATTGGAAAAGCAAATGCTGGCCGGGCTGTCCTCTCCCAACTGTACTTCTTCAAACAACTTGAATGGTGGTCCAGTAGGAATAGGTCCTGGTCCTTTCTCTGGCCTATTTCAAATTTCTGGTCTTGCTTGTTCATTGGCATATTTGGTTATAGTTGCACGTATAGTGCGTAAGCATCTGCGGGACTTGAGCTACACGCGACTACAAGCAATGTTAACCGACACAAAAATGTGGAGACACTTAGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

861

Amino Acids

95.61

Weight (kDa)

7.93

Isoelectric Point (pI)

29.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	35 - 361	7.9e-10	Periplasmic binding protein
ANF_receptor	PF01094	52 - 394	1.3e-39	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	473 - 553	2.5e-06	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	475 - 783	3.7e-10	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	571 - 821	1.1e-16	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005524)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g34350 FvH4_2g34350 FvH4_2g34350 FvH4_2g34350 FvH4_2g34350 FvH4_2g34370
malus_domestica	MD15G1082100.v1.1
prunus_persica	Prupe.1G434800_v2.0.a1
pyrus_communis	pycom15g07690
rosa_chinensis	RchiOBHm_Chr6g0308001 RchiOBHm_Chr6g0308011
rosa_laevigata	RLG00000010676 RLG00000010677 RLG00000010678 RLG00000010679
rosa_multiflora	Rmu_sc0006237.1_g000009 Rmu_sc0006237.1_g000010
rosa_roxburghii	Rroxscaffold_7G00160680 Rroxscaffold_7G00160690
rosa_rugosa	Rorug06G0362900 Rorug06G0363000
rosa_samantha	Rh6BG482500 Rh6BG482700 Rh6CG487800 Rh6CG488000 Rh6DG474100 Rh6DG474200
rosa_wichuraiana	Rw6G041170 Rw6G041180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	901
Acc36I	ACCTGC	1 cut(s)	206
AccI	GTMKAC	3 cut(s)	194, 1091, 1545
AccII	CGCG	3 cut(s)	138, 406, 2533
AciI	CCGC	4 cut(s)	404, 2156, 2244, 2515
AclWI	GGATC	4 cut(s)	394, 566, 1350, 1403
AcoI	YGGCCR	3 cut(s)	294, 1929, 2355
AcsI	RAATTY	5 cut(s)	539, 1083, 1124, 1613, 2448
AcuI	CTGAAG	3 cut(s)	537, 1290, 1697
AfaI	GTAC	2 cut(s)	953, 2380
AfiI	CCNNNNNNNGG	6 cut(s)	555, 1220, 1320, 1373, 1514, 2435
AjnI	CCWGG	4 cut(s)	1294, 1370, 1411, 2422
AloI	GAACNNNNNNTCC	2 cut(s)	1706, 1738
Alw26I	GTCTC	5 cut(s)	887, 1080, 1112, 1782, 2563
AlwI	GGATC	4 cut(s)	394, 566, 1350, 1403
AlwNI	CAGNNNCTG	1 cut(s)	2228
Ama87I	CYCGRG	4 cut(s)	246, 1026, 1435, 1636
AoxI	GGCC	8 cut(s)	294, 425, 1017, 1292, 1929, 2225, 2355, 2437
ApeKI	GCWGC	8 cut(s)	257, 260, 279, 326, 479, 1463, 1565, 2153
ApoI	RAATTY	5 cut(s)	539, 1083, 1124, 1613, 2448
ArsI	GACNNNNNNTTYG	2 cut(s)	2440, 2472
Asp700I	GAANNNNTTC	2 cut(s)	1476, 1866
AspA2I	CCTAGG	1 cut(s)	1102
AspLEI	GCGC	2 cut(s)	203, 531
AspS9I	GGNCC	5 cut(s)	1017, 2225, 2405, 2420, 2426
AsuC2I	CCSGG	2 cut(s)	650, 2359
AsuHPI	GGTGA	8 cut(s)	346, 523, 1546, 1586, 1699, 1828, 1942, 2129
AsuII	TTCGAA	2 cut(s)	750, 1581
AsuNHI	GCTAGC	1 cut(s)	1141
AvaI	CYCGRG	4 cut(s)	246, 1026, 1435, 1636
AvaII	GGWCC	3 cut(s)	2405, 2420, 2426
AvrII	CCTAGG	1 cut(s)	1102
BaeI	ACNNNNGTAYC	2 cut(s)	935, 968
BalI	TGGCCA	1 cut(s)	296
BanII	GRGCYC	1 cut(s)	1885
BbsI	GAAGAC	3 cut(s)	627, 697, 2179
BbvCI	CCTCAGC	1 cut(s)	275
BbvI	GCAGC	8 cut(s)	244, 272, 291, 338, 491, 1475, 1577, 2140
BccI	CCATC	9 cut(s)	380, 634, 760, 769, 1192, 1234, 1246, 1642, 1847
BceAI	ACGGC	1 cut(s)	1944
BcgI	CGANNNNNNTGC	4 cut(s)	881, 915, 1191, 1225
BciT130I	CCWGG	4 cut(s)	1296, 1372, 1413, 2424
BcnI	CCSGG	2 cut(s)	650, 2359
BcoDI	GTCTC	5 cut(s)	887, 1080, 1112, 1782, 2563
BfmI	CTRYAG	4 cut(s)	258, 516, 947, 1189
BfuAI	ACCTGC	1 cut(s)	206
BglII	AGATCT	1 cut(s)	181
BisI	GCNGC	9 cut(s)	258, 261, 280, 327, 480, 1464, 1566, 2154, 2157
BlnI	CCTAGG	1 cut(s)	1102
BlsI	GCNGC	9 cut(s)	259, 262, 281, 328, 481, 1465, 1567, 2155, 2158
Bme1390I	CCNGG	6 cut(s)	650, 1296, 1372, 1413, 2359, 2424
Bme18I	GGWCC	3 cut(s)	2405, 2420, 2426
BmeT110I	CYCGRG	4 cut(s)	246, 1026, 1435, 1636
BmgT120I	GGNCC	5 cut(s)	1017, 2225, 2405, 2420, 2426
BmiI	GGNNCC	3 cut(s)	1018, 1365, 1899
BmrFI	CCNGG	6 cut(s)	650, 1296, 1372, 1413, 2359, 2424
BmrI	ACTGGG	1 cut(s)	1023
BmsI	GCATC	5 cut(s)	643, 997, 1440, 1552, 2518
BmtI	GCTAGC	1 cut(s)	1145
BmuI	ACTGGG	1 cut(s)	1023
BoxI	GACNNNNGTC	1 cut(s)	1331
BpiI	GAAGAC	3 cut(s)	627, 697, 2179
Bpu10I	CCTNAGC	1 cut(s)	275
Bpu14I	TTCGAA	2 cut(s)	750, 1581
BpuEI	CTTGAG	4 cut(s)	216, 1664, 1956, 2542
BpuMI	CCSGG	2 cut(s)	650, 2359
Bsa29I	ATCGAT	2 cut(s)	123, 1449
BsaAI	YACGTR	2 cut(s)	2215, 2495
BsaBI	GATNNNNATC	1 cut(s)	1599
BsaI	GGTCTC	1 cut(s)	1112
BsaJI	CCNNGG	6 cut(s)	54, 162, 1102, 1371, 1710, 1901
BsaWI	WCCGGW	1 cut(s)	1330
Bsc4I	CCNNNNNNNGG	6 cut(s)	555, 1220, 1320, 1373, 1514, 2435
Bse1I	ACTGG	4 cut(s)	1018, 1545, 1658, 2408
Bse3DI	GCAATG	5 cut(s)	476, 1040, 1824, 2307, 2550
Bse8I	GATNNNNATC	1 cut(s)	1599
BseBI	CCWGG	4 cut(s)	1296, 1372, 1413, 2424
BseCI	ATCGAT	2 cut(s)	123, 1449
BseDI	CCNNGG	6 cut(s)	54, 162, 1102, 1371, 1710, 1901
BseGI	GGATG	6 cut(s)	435, 626, 658, 1257, 1695, 1839
BseJI	GATNNNNATC	1 cut(s)	1599
BseLI	CCNNNNNNNGG	6 cut(s)	555, 1220, 1320, 1373, 1514, 2435
BseMI	GCAATG	5 cut(s)	476, 1040, 1824, 2307, 2550
BseMII	CTCAG	8 cut(s)	142, 255, 289, 330, 612, 1077, 1123, 2307
BseNI	ACTGG	4 cut(s)	1018, 1545, 1658, 2408
BseRI	GAGGAG	3 cut(s)	263, 560, 2265
BseXI	GCAGC	8 cut(s)	244, 272, 291, 338, 491, 1475, 1577, 2140
BsgI	GTGCAG	1 cut(s)	338
Bsh1236I	CGCG	3 cut(s)	138, 406, 2533
BshFI	GGCC	8 cut(s)	296, 427, 1019, 1294, 1931, 2227, 2357, 2439
BshVI	ATCGAT	2 cut(s)	123, 1449
BsiHKCI	CYCGRG	4 cut(s)	246, 1026, 1435, 1636
BsiSI	CCGG	3 cut(s)	650, 1331, 2358
BslFI	GGGAC	1 cut(s)	2531
BslI	CCNNNNNNNGG	6 cut(s)	555, 1220, 1320, 1373, 1514, 2435
BsmAI	GTCTC	5 cut(s)	887, 1080, 1112, 1782, 2563
BsmFI	GGGAC	1 cut(s)	2531
BsmI	GAATGC	2 cut(s)	1379, 2023
BsnI	GGCC	8 cut(s)	296, 427, 1019, 1294, 1931, 2227, 2357, 2439
Bso31I	GGTCTC	1 cut(s)	1112
BsoBI	CYCGRG	4 cut(s)	246, 1026, 1435, 1636
Bsp119I	TTCGAA	2 cut(s)	750, 1581
Bsp1286I	GDGCHC	1 cut(s)	1885
Bsp143I	GATC	8 cut(s)	124, 181, 399, 407, 558, 1342, 1408, 1594
Bsp19I	CCATGG	2 cut(s)	162, 1901
BspACI	CCGC	4 cut(s)	404, 2156, 2244, 2515
BspANI	GGCC	8 cut(s)	296, 427, 1019, 1294, 1931, 2227, 2357, 2439
BspCNI	CTCAG	8 cut(s)	141, 254, 288, 331, 611, 1076, 1122, 2308
BspDI	ATCGAT	2 cut(s)	123, 1449
BspFNI	CGCG	3 cut(s)	138, 406, 2533
BspLI	GGNNCC	3 cut(s)	1018, 1365, 1899
BspMAI	CTGCAG	2 cut(s)	262, 520
BspMI	ACCTGC	1 cut(s)	206
BspOI	GCTAGC	1 cut(s)	1145
BspPI	GGATC	4 cut(s)	394, 566, 1350, 1403
BspQI	GCTCTTC	1 cut(s)	663
BspT104I	TTCGAA	2 cut(s)	750, 1581
BspTNI	GGTCTC	1 cut(s)	1112
BsrDI	GCAATG	5 cut(s)	476, 1040, 1824, 2307, 2550
BsrI	ACTGG	4 cut(s)	1018, 1545, 1658, 2408
BssECI	CCNNGG	6 cut(s)	54, 162, 1102, 1371, 1710, 1901
BssMI	GATC	8 cut(s)	124, 181, 399, 407, 558, 1342, 1408, 1594
BssT1I	CCWWGG	4 cut(s)	54, 162, 1102, 1901
Bst2UI	CCWGG	4 cut(s)	1296, 1372, 1413, 2424
Bst4CI	ACNGT	7 cut(s)	316, 915, 951, 1136, 1223, 2026, 2378
Bst6I	CTCTTC	3 cut(s)	61, 663, 1850
BstAPI	GCANNNNNTGC	1 cut(s)	257
BstBAI	YACGTR	2 cut(s)	2215, 2495
BstBI	TTCGAA	2 cut(s)	750, 1581
BstC8I	GCNNGC	6 cut(s)	817, 1118, 1143, 1701, 1945, 2355
BstDSI	CCRYGG	3 cut(s)	162, 1710, 1901
BstF5I	GGATG	6 cut(s)	435, 626, 658, 1257, 1695, 1839
BstFNI	CGCG	3 cut(s)	138, 406, 2533
BstHHI	GCGC	2 cut(s)	203, 531
BstKTI	GATC	8 cut(s)	127, 184, 402, 410, 561, 1345, 1411, 1597
BstMAI	GTCTC	5 cut(s)	887, 1080, 1112, 1782, 2563
BstMBI	GATC	8 cut(s)	124, 181, 399, 407, 558, 1342, 1408, 1594
BstMWI	GCNNNNNNNGC	9 cut(s)	257, 998, 1007, 1016, 1376, 1498, 1672, 1928, 2359
BstNI	CCWGG	4 cut(s)	1296, 1372, 1413, 2424
BstNSI	RCATGY	2 cut(s)	469, 1120
BstPAI	GACNNNNGTC	1 cut(s)	1331
BstSCI	CCNGG	6 cut(s)	648, 1294, 1370, 1411, 2357, 2422
BstSFI	CTRYAG	4 cut(s)	258, 516, 947, 1189
BstUI	CGCG	3 cut(s)	138, 406, 2533
BstV1I	GCAGC	8 cut(s)	244, 272, 291, 338, 491, 1475, 1577, 2140
BstV2I	GAAGAC	3 cut(s)	627, 697, 2179
BstX2I	RGATCY	1 cut(s)	181
BstXI	CCANNNNNNTGG	2 cut(s)	169, 1323
BstYI	RGATCY	1 cut(s)	181
Bsu15I	ATCGAT	2 cut(s)	123, 1449
BsuRI	GGCC	8 cut(s)	296, 427, 1019, 1294, 1931, 2227, 2357, 2439
BsuTUI	ATCGAT	2 cut(s)	123, 1449
BtgI	CCRYGG	3 cut(s)	162, 1710, 1901
BtsCI	GGATG	6 cut(s)	435, 626, 658, 1257, 1695, 1839
BtsI	GCAGTG	3 cut(s)	390, 1038, 1938
BtsIMutI	CAGTG	5 cut(s)	390, 1038, 1117, 1665, 1938
BveI	ACCTGC	1 cut(s)	206
Cac8I	GCNNGC	6 cut(s)	817, 1118, 1143, 1701, 1945, 2355
CaiI	CAGNNNCTG	1 cut(s)	2228
CfoI	GCGC	2 cut(s)	203, 531
Cfr13I	GGNCC	5 cut(s)	1017, 2225, 2405, 2420, 2426
ClaI	ATCGAT	2 cut(s)	123, 1449
CseI	GACGC	2 cut(s)	402, 891
Csp6I	GTAC	2 cut(s)	952, 2379
CviQI	GTAC	2 cut(s)	952, 2379
DpnI	GATC	8 cut(s)	126, 183, 401, 409, 560, 1344, 1410, 1596
DpnII	GATC	8 cut(s)	124, 181, 399, 407, 558, 1342, 1408, 1594
DraI	TTTAAA	1 cut(s)	1459
DrdI	GACNNNNNNGTC	1 cut(s)	901
DseDI	GACNNNNNNGTC	1 cut(s)	901
EaeI	YGGCCR	3 cut(s)	294, 1929, 2355
Eam1104I	CTCTTC	3 cut(s)	61, 663, 1850
EarI	CTCTTC	3 cut(s)	61, 663, 1850
Eco130I	CCWWGG	4 cut(s)	54, 162, 1102, 1901
Eco24I	GRGCYC	1 cut(s)	1885
Eco31I	GGTCTC	1 cut(s)	1112
Eco32I	GATATC	1 cut(s)	454
Eco47I	GGWCC	3 cut(s)	2405, 2420, 2426
Eco57I	CTGAAG	3 cut(s)	537, 1290, 1697
Eco88I	CYCGRG	4 cut(s)	246, 1026, 1435, 1636
EcoO109I	RGGNCCY	2 cut(s)	2225, 2420
EcoRI	GAATTC	2 cut(s)	1124, 1613
EcoRII	CCWGG	4 cut(s)	1294, 1370, 1411, 2422
EcoRV	GATATC	1 cut(s)	454
EcoT14I	CCWWGG	4 cut(s)	54, 162, 1102, 1901
EcoT38I	GRGCYC	1 cut(s)	1885
ErhI	CCWWGG	4 cut(s)	54, 162, 1102, 1901
FalI	AAGNNNNNCTT	2 cut(s)	315, 347
FaqI	GGGAC	1 cut(s)	2531
FauI	CCCGC	2 cut(s)	411, 2508
FblI	GTMKAC	3 cut(s)	194, 1091, 1545
Fnu4HI	GCNGC	9 cut(s)	258, 261, 280, 327, 480, 1464, 1566, 2154, 2157
FokI	GGATG	6 cut(s)	422, 613, 665, 1264, 1702, 1826
FriOI	GRGCYC	1 cut(s)	1885
Fsp4HI	GCNGC	9 cut(s)	258, 261, 280, 327, 480, 1464, 1566, 2154, 2157
GlaI	GCGC	2 cut(s)	202, 530
GluI	GCNGC	9 cut(s)	258, 261, 280, 327, 480, 1464, 1566, 2154, 2157
HaeIII	GGCC	8 cut(s)	296, 427, 1019, 1294, 1931, 2227, 2357, 2439
HapII	CCGG	3 cut(s)	650, 1331, 2358
HgaI	GACGC	2 cut(s)	402, 891
HhaI	GCGC	2 cut(s)	203, 531
Hin6I	GCGC	2 cut(s)	201, 529
HinP1I	GCGC	2 cut(s)	201, 529
HincII	GTYRAC	4 cut(s)	195, 595, 835, 2550
HindII	GTYRAC	4 cut(s)	195, 595, 835, 2550
HinfI	GANTC	6 cut(s)	108, 227, 827, 1327, 1915, 1963
HpaI	GTTAAC	2 cut(s)	835, 2550
HpaII	CCGG	3 cut(s)	650, 1331, 2358
HphI	GGTGA	8 cut(s)	346, 523, 1546, 1586, 1699, 1828, 1942, 2129
Hpy188I	TCNGA	9 cut(s)	305, 340, 601, 911, 998, 1066, 1270, 1594, 2296
Hpy188III	TCNNGA	4 cut(s)	824, 892, 1129, 1437
Hpy99I	CGWCG	1 cut(s)	1159
HpyAV	CCTTC	6 cut(s)	28, 667, 830, 1519, 1672, 1714
HpyCH4III	ACNGT	7 cut(s)	316, 915, 951, 1136, 1223, 2026, 2378
HpyCH4IV	ACGT	5 cut(s)	1157, 1173, 1398, 2214, 2494
HpyF10VI	GCNNNNNNNGC	9 cut(s)	257, 998, 1007, 1016, 1376, 1498, 1672, 1928, 2359
HpySE526I	ACGT	5 cut(s)	1157, 1173, 1398, 2214, 2494
HspAI	GCGC	2 cut(s)	201, 529
KspAI	GTTAAC	2 cut(s)	835, 2550
Kzo9I	GATC	8 cut(s)	124, 181, 399, 407, 558, 1342, 1408, 1594
LguI	GCTCTTC	1 cut(s)	663
LmnI	GCTCC	1 cut(s)	1903
Lsp1109I	GCAGC	8 cut(s)	244, 272, 291, 338, 491, 1475, 1577, 2140
LweI	GCATC	5 cut(s)	643, 997, 1440, 1552, 2518
MaeII	ACGT	5 cut(s)	1157, 1173, 1398, 2214, 2494
MalI	GATC	8 cut(s)	126, 183, 401, 409, 560, 1344, 1410, 1596
MboI	GATC	8 cut(s)	124, 181, 399, 407, 558, 1342, 1408, 1594
MfeI	CAATTG	1 cut(s)	1876
MflI	RGATCY	1 cut(s)	181
MhlI	GDGCHC	1 cut(s)	1885
MlsI	TGGCCA	1 cut(s)	296
MluNI	TGGCCA	1 cut(s)	296
MlyI	GAGTC	2 cut(s)	1321, 1957
MmeI	TCCRAC	1 cut(s)	283
Mox20I	TGGCCA	1 cut(s)	296
MroXI	GAANNNNTTC	2 cut(s)	1476, 1866
MscI	TGGCCA	1 cut(s)	296
MseI	TTAA	8 cut(s)	189, 834, 876, 1170, 1352, 1458, 1769, 2549
MslI	CAYNNNNRTG	6 cut(s)	167, 713, 1172, 1715, 1955, 2561
Msp20I	TGGCCA	1 cut(s)	296
MspA1I	CMGCKG	4 cut(s)	257, 737, 1466, 1568
MspI	CCGG	3 cut(s)	650, 1331, 2358
MspR9I	CCNGG	6 cut(s)	650, 1296, 1372, 1413, 2359, 2424
MunI	CAATTG	1 cut(s)	1876
Mva1269I	GAATGC	2 cut(s)	1379, 2023
MvaI	CCWGG	4 cut(s)	1296, 1372, 1413, 2424
MvnI	CGCG	3 cut(s)	138, 406, 2533
MwoI	GCNNNNNNNGC	9 cut(s)	257, 998, 1007, 1016, 1376, 1498, 1672, 1928, 2359
NciI	CCSGG	2 cut(s)	650, 2359
NcoI	CCATGG	2 cut(s)	162, 1901
NdeII	GATC	8 cut(s)	124, 181, 399, 407, 558, 1342, 1408, 1594
NheI	GCTAGC	1 cut(s)	1141
NlaIV	GGNNCC	3 cut(s)	1018, 1365, 1899
NmuCI	GTSAC	7 cut(s)	414, 511, 799, 1033, 1834, 2117, 2215
NspI	RCATGY	2 cut(s)	469, 1120
NspV	TTCGAA	2 cut(s)	750, 1581
PaeI	GCATGC	1 cut(s)	1120
PaeR7I	CTCGAG	2 cut(s)	246, 1026
PciSI	GCTCTTC	1 cut(s)	663
PcsI	WCGNNNNNNNCGW	2 cut(s)	747, 2500
PctI	GAATGC	2 cut(s)	1379, 2023
PdmI	GAANNNNTTC	2 cut(s)	1476, 1866
PfeI	GAWTC	4 cut(s)	108, 227, 827, 1915
PkrI	GCNGC	9 cut(s)	259, 262, 281, 328, 481, 1465, 1567, 2155, 2158
PleI	GAGTC	2 cut(s)	1321, 1957
PpsI	GAGTC	2 cut(s)	1321, 1957
Ppu21I	YACGTR	2 cut(s)	2215, 2495
PpuMI	RGGWCCY	1 cut(s)	2420
PshAI	GACNNNNGTC	1 cut(s)	1331
Psp5II	RGGWCCY	1 cut(s)	2420
Psp6I	CCWGG	4 cut(s)	1294, 1370, 1411, 2422
PspGI	CCWGG	4 cut(s)	1294, 1370, 1411, 2422
PspN4I	GGNNCC	3 cut(s)	1018, 1365, 1899
PspPI	GGNCC	5 cut(s)	1017, 2225, 2405, 2420, 2426
PspPPI	RGGWCCY	1 cut(s)	2420
PspXI	VCTCGAGB	2 cut(s)	246, 1026
PstI	CTGCAG	2 cut(s)	262, 520
PstNI	CAGNNNCTG	1 cut(s)	2228
PsuI	RGATCY	1 cut(s)	181
PvuII	CAGCTG	4 cut(s)	257, 737, 1466, 1568
RsaI	GTAC	2 cut(s)	953, 2380
RsaNI	GTAC	2 cut(s)	952, 2379
RseI	CAYNNNNRTG	6 cut(s)	167, 713, 1172, 1715, 1955, 2561
SalI	GTCGAC	1 cut(s)	193
SapI	GCTCTTC	1 cut(s)	663
SaqAI	TTAA	8 cut(s)	189, 834, 876, 1170, 1352, 1458, 1769, 2549
SatI	GCNGC	9 cut(s)	258, 261, 280, 327, 480, 1464, 1566, 2154, 2157
Sau3AI	GATC	8 cut(s)	124, 181, 399, 407, 558, 1342, 1408, 1594
Sau96I	GGNCC	5 cut(s)	1017, 2225, 2405, 2420, 2426
SchI	GAGTC	2 cut(s)	1321, 1957
ScrFI	CCNGG	6 cut(s)	650, 1296, 1372, 1413, 2359, 2424
SduI	GDGCHC	1 cut(s)	1885
SfaNI	GCATC	5 cut(s)	643, 997, 1440, 1552, 2518
SfcI	CTRYAG	4 cut(s)	258, 516, 947, 1189
Sfr274I	CTCGAG	2 cut(s)	246, 1026
SfuI	TTCGAA	2 cut(s)	750, 1581
SinI	GGWCC	3 cut(s)	2405, 2420, 2426
SlaI	CTCGAG	2 cut(s)	246, 1026
SmiMI	CAYNNNNRTG	6 cut(s)	167, 713, 1172, 1715, 1955, 2561
SmlI	CTYRAG	6 cut(s)	231, 246, 1026, 1643, 1971, 2521
SmoI	CTYRAG	6 cut(s)	231, 246, 1026, 1643, 1971, 2521
SphI	GCATGC	1 cut(s)	1120
SsiI	CCGC	4 cut(s)	404, 2156, 2244, 2515
StyD4I	CCNGG	6 cut(s)	648, 1294, 1370, 1411, 2357, 2422
StyI	CCWWGG	4 cut(s)	54, 162, 1102, 1901
TaaI	ACNGT	7 cut(s)	316, 915, 951, 1136, 1223, 2026, 2378
TaiI	ACGT	5 cut(s)	1160, 1176, 1401, 2217, 2497
TaqII	GACCGA	1 cut(s)	2081
TatI	WGTACW	1 cut(s)	2378
TauI	GCSGC	1 cut(s)	2159
TfiI	GAWTC	4 cut(s)	108, 227, 827, 1915
Tru1I	TTAA	8 cut(s)	189, 834, 876, 1170, 1352, 1458, 1769, 2549
Tru9I	TTAA	8 cut(s)	189, 834, 876, 1170, 1352, 1458, 1769, 2549
TscAI	CASTG	5 cut(s)	397, 1038, 1117, 1665, 1945
TseFI	GTSAC	7 cut(s)	414, 511, 799, 1033, 1834, 2117, 2215
TseI	GCWGC	8 cut(s)	257, 260, 279, 326, 479, 1463, 1565, 2153
Tsp45I	GTSAC	7 cut(s)	414, 511, 799, 1033, 1834, 2117, 2215
TspGWI	ACGGA	2 cut(s)	957, 1727
TspRI	CASTG	5 cut(s)	397, 1038, 1117, 1665, 1945
VpaK11BI	GGWCC	3 cut(s)	2405, 2420, 2426
XapI	RAATTY	5 cut(s)	539, 1083, 1124, 1613, 2448
XceI	RCATGY	2 cut(s)	469, 1120
XhoI	CTCGAG	2 cut(s)	246, 1026
XmaJI	CCTAGG	1 cut(s)	1102
XmiI	GTMKAC	3 cut(s)	194, 1091, 1545
XmnI	GAANNNNTTC	2 cut(s)	1476, 1866

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.