FvH4_2g37450

Zn_pept

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008016
Physical Location & Seq
Forward (+)
27287372 .. 27291699
4328 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g37450.t1

Sequence Viewer

Length: 1305 bp
ATGGCTAACCAGGTTCCTCTGTGGTCTCCGTTGTGCATCAGTTTGTGCTTGTTTGCTTGGTTTCAAGGTTTCTTCTCGATTGTCCATGGCGATAGTAATCCCTCTCAACCTTTTCCCACGCCCATTAACCGCGATCTCTACCATTCCAGTGGGGATTTGATGGAAGAGATTAAAGCTTTGGTAATTCGACACCCGGATAAACTCACTTTGGATACAATTAAGGCTAGAAACAAGGGTTACTCTGCTGAGATTGATGTAATTACTTATTGTCGGAGAAGGCAGGAGGCCAATGACAAGCCAAAGTTTCGGATTCTTCTTAGTTTTGGGCAGCATGGAAGGGAGCTCATTACATCTGAAGTTGCTTTGCGAATCCTTTCAATCTTGAGTAAAGAAAAGTTTCTGCCCGACTTGGACCTAGCCTCCTTAGACCAGACCCTTGACAATCTTGTGATAAAGGTGGTGCCTATGGAAAACCTAAATGGCCGCAGACTTGTAGAAGCTGGAGACCTCTGCGAGAGGAGAAATGGGAGAGGAGTTGATCTAAACCGTAATTGGAGCGTAGATTGGGGCAAAAAAGAGAAGGATTATGATCCGTATGAGGAAAATCCTGGGACTGCTCCTTTCAGTGAGCCTGAAGCTCAAATAATGCGGAAAATTGCAGTGTCATTTGATCCGCACATATGGGTTAATATTCACTCTGGGATGGAGGCTTTATTTATGCCATATGATCATAAGAACACAACCCCTGATGGAGTTCCCTCGCAAAAAATGAAATCATTGCTTGAAGAACTGAACCAACTGCATTGCCAGAAACGTTGCATGATTGGGTCTGGTGGAGGTTCCGTTGGGTATTTGGCACATGGGACAGCAACTGATTTTATGTTTGACATTGTAAAGGTGCCCATGGCGTTCACCTTTGAGATATATGGAGATGGAGCAGCAACATCAAGAGATTGCTTTAAAATGTTCAATCCCACCGACTTCGGTACCTTCAACAGGGTTCTCCATGAATGGTCTGCTGCATTTTTCACAATTTTTAAACTGGGGCCAAACTTGCTCGGTTCAAATTATTCAAAGCCCGCCGTAGACAAGTGGGTGTCCATAGATGAGTATCTTGATGGGTACTTGGTCGAGAGGAGTAGTAGATATGGAAAGAAGACGGAGGTGCTTGATCTAGGAATGCAGGAGATAAGAACATATTTCAGGCTCTTCTTGTTATCCTCAGTTCTGTTACTGTTCATGTTCTGTTCCAGAATTTCAAAAAGCAAGTTTTCTAGACCAATTGTTTCTGCTATTCCACTCTGA

Protein Analysis

435

Amino Acids

49.34

Weight (kDa)

6.8

Isoelectric Point (pI)

36.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M14 PF00246 56 - 321 3.4e-26 Zinc carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 986
AccB1I GGYRCC 3 cut(s) 460, 898, 986
AccI GTMKAC 1 cut(s) 1086
AccII CGCG 1 cut(s) 132
AciI CCGC 5 cut(s) 130, 484, 649, 674, 1080
AclI AACGTT 1 cut(s) 814
AclWI GGATC 2 cut(s) 584, 665
AcoI YGGCCR 1 cut(s) 481
AcsI RAATTY 1 cut(s) 1254
AcuI CTGAAG 2 cut(s) 375, 654
AfaI GTAC 2 cut(s) 988, 1124
AfiI CCNNNNNNNGG 1 cut(s) 996
AgsI TTSAA 8 cut(s) 65, 378, 785, 970, 994, 1065, 1074, 1260
AjnI CCWGG 2 cut(s) 9, 607
AluBI AGCT 4 cut(s) 176, 343, 500, 638
AluI AGCT 4 cut(s) 176, 343, 500, 638
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 2 cut(s) 30, 498
AlwI GGATC 2 cut(s) 584, 665
AlwNI CAGNNNCTG 1 cut(s) 872
AoxI GGCC 3 cut(s) 285, 481, 1046
ApeKI GCWGC 3 cut(s) 328, 938, 1019
ApoI RAATTY 1 cut(s) 1254
Asp700I GAANNNNTTC 2 cut(s) 373, 396
Asp718I GGTACC 1 cut(s) 986
AspS9I GGNCC 2 cut(s) 412, 1046
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 1 cut(s) 904
AvaII GGWCC 1 cut(s) 412
BaeGI GKGCMC 1 cut(s) 903
BaeI ACNNNNGTAYC 2 cut(s) 970, 1003
BanI GGYRCC 3 cut(s) 460, 898, 986
BanII GRGCYC 1 cut(s) 345
BbsI GAAGAC 1 cut(s) 1163
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 3 cut(s) 340, 950, 1006
BccI CCATC 5 cut(s) 154, 697, 743, 926, 1112
BceAI ACGGC 1 cut(s) 1067
BciT130I CCWGG 2 cut(s) 11, 609
BciVI GTATCC 1 cut(s) 205
BclI TGATCA 1 cut(s) 727
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 2 cut(s) 30, 498
BfaI CTAG 4 cut(s) 225, 416, 1175, 1275
BfuI GTATCC 1 cut(s) 205
BisI GCNGC 4 cut(s) 329, 484, 939, 1020
BlsI GCNGC 4 cut(s) 330, 485, 940, 1021
Bme1390I CCNGG 3 cut(s) 11, 194, 609
Bme18I GGWCC 1 cut(s) 412
BmgT120I GGNCC 2 cut(s) 412, 1046
BmiI GGNNCC 6 cut(s) 15, 462, 841, 900, 988, 1047
BmrFI CCNGG 3 cut(s) 11, 194, 609
BmrI ACTGGG 1 cut(s) 1052
BmsI GCATC 1 cut(s) 45
BmuI ACTGGG 1 cut(s) 1052
BpiI GAAGAC 1 cut(s) 1163
BpmI CTGGAG 1 cut(s) 522
BpuEI CTTGAG 1 cut(s) 403
BpuMI CCSGG 1 cut(s) 194
BsaBI GATNNNNATC 3 cut(s) 96, 588, 1110
BsaI GGTCTC 2 cut(s) 30, 498
BsaJI CCNNGG 3 cut(s) 85, 608, 903
BsaXI ACNNNNNCTCC 4 cut(s) 404, 434, 828, 858
Bsc4I CCNNNNNNNGG 1 cut(s) 996
Bse1I ACTGG 2 cut(s) 147, 1047
Bse3DI GCAATG 2 cut(s) 776, 802
Bse8I GATNNNNATC 3 cut(s) 96, 588, 1110
BseBI CCWGG 2 cut(s) 11, 609
BseDI CCNNGG 3 cut(s) 85, 608, 903
BseGI GGATG 1 cut(s) 708
BseJI GATNNNNATC 3 cut(s) 96, 588, 1110
BseLI CCNNNNNNNGG 1 cut(s) 996
BseMI GCAATG 2 cut(s) 776, 802
BseMII CTCAG 2 cut(s) 237, 1236
BseNI ACTGG 2 cut(s) 147, 1047
BseRI GAGGAG 3 cut(s) 532, 546, 1150
BseSI GKGCMC 1 cut(s) 903
BseXI GCAGC 3 cut(s) 340, 950, 1006
Bsh1236I CGCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 287, 483, 1048
BshNI GGYRCC 3 cut(s) 460, 898, 986
BsiHKAI GWGCWC 1 cut(s) 345
BsiSI CCGG 1 cut(s) 194
BslFI GGGAC 2 cut(s) 625, 877
BslI CCNNNNNNNGG 1 cut(s) 996
BsmAI GTCTC 2 cut(s) 30, 498
BsmFI GGGAC 2 cut(s) 625, 877
BsmI GAATGC 1 cut(s) 1185
BsnI GGCC 3 cut(s) 287, 483, 1048
Bso31I GGTCTC 2 cut(s) 30, 498
Bsp1286I GDGCHC 2 cut(s) 345, 903
Bsp143I GATC 6 cut(s) 133, 538, 589, 670, 727, 1171
Bsp19I CCATGG 2 cut(s) 85, 903
BspACI CCGC 5 cut(s) 130, 484, 649, 674, 1080
BspANI GGCC 3 cut(s) 287, 483, 1048
BspCNI CTCAG 2 cut(s) 238, 1235
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 6 cut(s) 15, 462, 841, 900, 988, 1047
BspPI GGATC 2 cut(s) 584, 665
BspQI GCTCTTC 1 cut(s) 1214
BspT107I GGYRCC 3 cut(s) 460, 898, 986
BspTNI GGTCTC 2 cut(s) 30, 498
BsrDI GCAATG 2 cut(s) 776, 802
BsrI ACTGG 2 cut(s) 147, 1047
BssECI CCNNGG 3 cut(s) 85, 608, 903
BssMI GATC 6 cut(s) 133, 538, 589, 670, 727, 1171
BssT1I CCWWGG 2 cut(s) 85, 903
Bst2UI CCWGG 2 cut(s) 11, 609
Bst4CI ACNGT 2 cut(s) 548, 1236
Bst6I CTCTTC 2 cut(s) 159, 1214
BstC8I GCNNGC 1 cut(s) 1080
BstDEI CTNAG 4 cut(s) 246, 317, 424, 1222
BstDSI CCRYGG 2 cut(s) 85, 903
BstENI CCTNNNNNAGG 1 cut(s) 994
BstF5I GGATG 1 cut(s) 708
BstFNI CGCG 1 cut(s) 132
BstKTI GATC 6 cut(s) 136, 541, 592, 673, 730, 1174
BstMAI GTCTC 2 cut(s) 30, 498
BstMBI GATC 6 cut(s) 133, 538, 589, 670, 727, 1171
BstMWI GCNNNNNNNGC 1 cut(s) 1054
BstNI CCWGG 2 cut(s) 11, 609
BstSCI CCNGG 3 cut(s) 9, 192, 607
BstSLI GKGCMC 1 cut(s) 903
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 3 cut(s) 340, 950, 1006
BstV2I GAAGAC 1 cut(s) 1163
BstXI CCANNNNNNTGG 1 cut(s) 149
BsuI GTATCC 1 cut(s) 205
BsuRI GGCC 3 cut(s) 287, 483, 1048
BtgI CCRYGG 2 cut(s) 85, 903
BtsCI GGATG 1 cut(s) 708
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 3 cut(s) 154, 631, 666
Cac8I GCNNGC 1 cut(s) 1080
CaiI CAGNNNCTG 1 cut(s) 872
Cfr13I GGNCC 2 cut(s) 412, 1046
CsiI ACCWGGT 1 cut(s) 9
Csp6I GTAC 2 cut(s) 987, 1123
CviAII CATG 7 cut(s) 86, 332, 820, 860, 904, 1007, 1240
CviQI GTAC 2 cut(s) 987, 1123
DdeI CTNAG 4 cut(s) 246, 317, 424, 1222
DpnI GATC 6 cut(s) 135, 540, 591, 672, 729, 1173
DpnII GATC 6 cut(s) 133, 538, 589, 670, 727, 1171
DraI TTTAAA 2 cut(s) 961, 1039
EaeI YGGCCR 1 cut(s) 481
Eam1104I CTCTTC 2 cut(s) 159, 1214
EarI CTCTTC 2 cut(s) 159, 1214
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 2 cut(s) 85, 903
Eco24I GRGCYC 1 cut(s) 345
Eco31I GGTCTC 2 cut(s) 30, 498
Eco47I GGWCC 1 cut(s) 412
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 2 cut(s) 375, 654
EcoICRI GAGCTC 1 cut(s) 343
EcoNI CCTNNNNNAGG 1 cut(s) 994
EcoRII CCWGG 2 cut(s) 9, 607
EcoT14I CCWWGG 2 cut(s) 85, 903
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 2 cut(s) 85, 903
FaeI CATG 7 cut(s) 89, 335, 823, 863, 907, 1010, 1243
FaqI GGGAC 2 cut(s) 625, 877
FatI CATG 7 cut(s) 85, 331, 819, 859, 903, 1006, 1239
FauI CCCGC 1 cut(s) 1087
FauNDI CATATG 2 cut(s) 680, 724
FbaI TGATCA 1 cut(s) 727
FblI GTMKAC 1 cut(s) 1086
Fnu4HI GCNGC 4 cut(s) 329, 484, 939, 1020
FokI GGATG 1 cut(s) 715
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 4 cut(s) 329, 484, 939, 1020
FspBI CTAG 4 cut(s) 225, 416, 1175, 1275
GluI GCNGC 4 cut(s) 329, 484, 939, 1020
GsuI CTGGAG 1 cut(s) 522
HaeIII GGCC 3 cut(s) 287, 483, 1048
HapII CCGG 1 cut(s) 194
Hin1II CATG 7 cut(s) 89, 335, 823, 863, 907, 1010, 1243
HindIII AAGCTT 1 cut(s) 174
HinfI GANTC 2 cut(s) 310, 369
HpaII CCGG 1 cut(s) 194
HphI GGTGA 1 cut(s) 904
Hpy166II GTNNAC 2 cut(s) 912, 1087
Hpy188I TCNGA 4 cut(s) 273, 309, 355, 1304
Hpy188III TCNNGA 7 cut(s) 76, 382, 948, 1115, 1132, 1251, 1275
Hpy8I GTNNAC 2 cut(s) 912, 1087
HpyAV CCTTC 4 cut(s) 270, 330, 574, 1000
HpyCH4III ACNGT 2 cut(s) 548, 1236
HpyCH4IV ACGT 1 cut(s) 814
HpyCH4V TGCA 6 cut(s) 36, 659, 802, 819, 1022, 1183
HpyF10VI GCNNNNNNNGC 1 cut(s) 1054
HpyF3I CTNAG 4 cut(s) 246, 317, 424, 1222
HpySE526I ACGT 1 cut(s) 814
Hsp92II CATG 7 cut(s) 89, 335, 823, 863, 907, 1010, 1243
KpnI GGTACC 1 cut(s) 990
Ksp22I TGATCA 1 cut(s) 727
Kzo9I GATC 6 cut(s) 133, 538, 589, 670, 727, 1171
LguI GCTCTTC 1 cut(s) 1214
LmnI GCTCC 4 cut(s) 340, 555, 622, 935
Lsp1109I GCAGC 3 cut(s) 340, 950, 1006
LweI GCATC 1 cut(s) 45
MabI ACCWGGT 1 cut(s) 9
MaeI CTAG 4 cut(s) 225, 416, 1175, 1275
MaeII ACGT 1 cut(s) 814
MaeIII GTNAC 2 cut(s) 236, 1230
MalI GATC 6 cut(s) 135, 540, 591, 672, 729, 1173
MboI GATC 6 cut(s) 133, 538, 589, 670, 727, 1171
MboII GAAGA 6 cut(s) 64, 176, 305, 797, 1168, 1201
MfeI CAATTG 1 cut(s) 1281
MhlI GDGCHC 2 cut(s) 345, 903
MluCI AATT 9 cut(s) 183, 216, 258, 550, 654, 1032, 1066, 1254, 1281
MmeI TCCRAC 1 cut(s) 251
MroXI GAANNNNTTC 2 cut(s) 373, 396
MseI TTAA 6 cut(s) 126, 171, 219, 687, 960, 1038
MslI CAYNNNNRTG 1 cut(s) 147
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 3 cut(s) 11, 194, 609
MunI CAATTG 1 cut(s) 1281
Mva1269I GAATGC 1 cut(s) 1185
MvaI CCWGG 2 cut(s) 11, 609
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 1 cut(s) 1054
NciI CCSGG 1 cut(s) 194
NcoI CCATGG 2 cut(s) 85, 903
NdeI CATATG 2 cut(s) 680, 724
NdeII GATC 6 cut(s) 133, 538, 589, 670, 727, 1171
NlaIII CATG 7 cut(s) 89, 335, 823, 863, 907, 1010, 1243
NlaIV GGNNCC 6 cut(s) 15, 462, 841, 900, 988, 1047
PciSI GCTCTTC 1 cut(s) 1214
PctI GAATGC 1 cut(s) 1185
PdmI GAANNNNTTC 2 cut(s) 373, 396
PfeI GAWTC 2 cut(s) 310, 369
PkrI GCNGC 4 cut(s) 330, 485, 940, 1021
Psp124BI GAGCTC 1 cut(s) 345
Psp1406I AACGTT 1 cut(s) 814
Psp6I CCWGG 2 cut(s) 9, 607
PspGI CCWGG 2 cut(s) 9, 607
PspN4I GGNNCC 6 cut(s) 15, 462, 841, 900, 988, 1047
PspPI GGNCC 2 cut(s) 412, 1046
PstNI CAGNNNCTG 1 cut(s) 872
RsaI GTAC 2 cut(s) 988, 1124
RsaNI GTAC 2 cut(s) 987, 1123
RseI CAYNNNNRTG 1 cut(s) 147
SacI GAGCTC 1 cut(s) 345
SapI GCTCTTC 1 cut(s) 1214
SaqAI TTAA 6 cut(s) 126, 171, 219, 687, 960, 1038
SatI GCNGC 4 cut(s) 329, 484, 939, 1020
Sau3AI GATC 6 cut(s) 133, 538, 589, 670, 727, 1171
Sau96I GGNCC 2 cut(s) 412, 1046
ScrFI CCNGG 3 cut(s) 11, 194, 609
SduI GDGCHC 2 cut(s) 345, 903
SexAI ACCWGGT 1 cut(s) 9
SfaNI GCATC 1 cut(s) 45
SinI GGWCC 1 cut(s) 412
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 382
SmoI CTYRAG 1 cut(s) 382
Sse9I AATT 9 cut(s) 183, 216, 258, 550, 654, 1032, 1066, 1254, 1281
SsiI CCGC 5 cut(s) 130, 484, 649, 674, 1080
SspI AATATT 1 cut(s) 691
SspMI CTAG 4 cut(s) 225, 416, 1175, 1275
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 3 cut(s) 9, 192, 607
StyI CCWWGG 2 cut(s) 85, 903
TaaI ACNGT 2 cut(s) 548, 1236
TaiI ACGT 1 cut(s) 817
TaqI TCGA 3 cut(s) 77, 187, 1131
TasI AATT 9 cut(s) 183, 216, 258, 550, 654, 1032, 1066, 1254, 1281
TauI GCSGC 1 cut(s) 486
TfiI GAWTC 2 cut(s) 310, 369
Tru1I TTAA 6 cut(s) 126, 171, 219, 687, 960, 1038
Tru9I TTAA 6 cut(s) 126, 171, 219, 687, 960, 1038
TscAI CASTG 3 cut(s) 154, 631, 666
TseI GCWGC 3 cut(s) 328, 938, 1019
TspDTI ATGAA 3 cut(s) 785, 1023, 1228
TspGWI ACGGA 4 cut(s) 18, 582, 832, 1175
TspRI CASTG 3 cut(s) 154, 631, 666
VpaK11BI GGWCC 1 cut(s) 412
XagI CCTNNNNNAGG 1 cut(s) 994
XapI RAATTY 1 cut(s) 1254
XbaI TCTAGA 1 cut(s) 1274
XmiI GTMKAC 1 cut(s) 1086
XmnI GAANNNNTTC 2 cut(s) 373, 396
XspI CTAG 4 cut(s) 225, 416, 1175, 1275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.