pycom15g00090

Zn_pept

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008016
Physical Location & Seq
Forward (+)
60275 .. 63555
3281 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g00090.1

Sequence Viewer

Length: 1314 bp
ATGGCTCATCTCCTTCCTCTCTCCTCTCCTTTATCCATCACTCTCTGCTTCTTTGCGTGGTTACAAGCTTTCGTGGTCGTCCATGGCAATAGTAATCTCACCCACCCTTCTCTCACCCCTATCAACCGCGATCTCTATCACTCCAGTGGGGATTTGATGGAAGAAATTAAAGCTCTCGTCTTTCGGCACCCAGAGGAGCTCTCTTTGGATACAATTAAATCTCAAAACAAGGGCTACTCTGCTGAGATCGCAATAGTTACCTATAGTCGGAGAAGTCAAGAGACTGATGACCGGCCAAAATTTCGGATCCTTCTTAGTTTTGGGCAGCATGGAAGGGAGCTTATTACATCCGAGCTTGCATTGCGAATCCTATCAATCTGTAGTAAAGAACAGTTTCTACCCAACCTGGACCCAGCCTCCTTAGACAGTACCCTGGACAAGCTCTTGATAAAGGTGGTTCCAATAGAAAACTTAAACGGCCGTAAACTTGTTGAAGCAGGAGACCTCTGTGAGAGAAGAAATGGGAGGGGAGTTGATCTCAACCGTAATTGGAGCGTAGATTGGGGCAAAAAAGAGAAGGATTATGATCGATATGAGGAATCTCCTGGAACTGCTCCTTTCAGCGAGCCTGAAACTCAGATAATACGGAAAGTTGCCGTGTCATTTGATCCACACATCTGGATTAATATACACTCTGGGATGGAGGCTCTGTTTATGCCGTATGACCATAAGAACACAACCCCAGATGGAGTTTCCTCCCACCAGATGAAGTCATTGCTTGAAGAACTCAACCAACTGCACTGCCAAAAGCGTTGCATGGTTGGGTCTGGTGGAGGTTCTGTTGGGTACCTGGCACATGGGACAGCAACTGATTTTATGTTCGACATTGTAAGGGTGCCAATGGCTTTCACCTTTGAGATATATGGAGATGGAGCGGCCTCATCAAGAGATTGCTTCAAAATGTTCAATCCCACCGACTTCGTTACCTTCAATAGAGTTCTCAATGAATGGTCTGCTGCATTTTTCACAATTTTTAAATTGGGGCCAGACCAGCTTGGTGAAAATTATTCAAAGGCTTCTGTGCCCACCTTATTAGACAAGTGGGTATCCATAGATGAGTATCTTGAAGGTTATTTAGTCGAGAGGAGTAGTAGATATGGAAAGAAGATGGAGGTGCTTGAACTAGGAATGCAGGAGATAAGAACATATTTCAGGCTCTTCTTGTTATCTTCTGTTCTGTTAATGTTCATGTTCTGTTCCAGAATTTCAAAAAGCAAGTGTGCTAGACCAATGGTTTCTGCTATTGCAATCTGA

Protein Analysis

438

Amino Acids

49.54

Weight (kDa)

6.2

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M14 PF00246 55 - 320 6.7e-25 Zinc carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 846
AccB1I GGYRCC 3 cut(s) 186, 846, 895
AccBSI CCGCTC 1 cut(s) 935
AccII CGCG 1 cut(s) 129
AciI CCGC 2 cut(s) 127, 935
AclWI GGATC 3 cut(s) 301, 314, 662
AcoI YGGCCR 2 cut(s) 293, 478
AcsI RAATTY 2 cut(s) 299, 1263
AfaI GTAC 2 cut(s) 430, 848
AfiI CCNNNNNNNGG 1 cut(s) 267
AgsI TTSAA 9 cut(s) 494, 782, 958, 967, 991, 1071, 1127, 1181, 1269
AjnI CCWGG 4 cut(s) 405, 432, 604, 849
AleI CACNNNNGTG 1 cut(s) 144
AluBI AGCT 7 cut(s) 68, 173, 199, 340, 355, 442, 1054
AluI AGCT 7 cut(s) 68, 173, 199, 340, 355, 442, 1054
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 2 cut(s) 275, 495
AlwI GGATC 3 cut(s) 301, 314, 662
AlwNI CAGNNNCTG 1 cut(s) 869
AoxI GGCC 4 cut(s) 293, 478, 936, 1043
ApeKI GCWGC 2 cut(s) 325, 1016
ApoI RAATTY 2 cut(s) 299, 1263
AseI ATTAAT 1 cut(s) 684
Asp700I GAANNNNTTC 1 cut(s) 393
Asp718I GGTACC 1 cut(s) 846
AspS9I GGNCC 2 cut(s) 409, 1043
AsuHPI GGTGA 4 cut(s) 91, 106, 901, 1070
AvaII GGWCC 1 cut(s) 409
BaeGI GKGCMC 1 cut(s) 1086
BamHI GGATCC 1 cut(s) 306
BanI GGYRCC 3 cut(s) 186, 846, 895
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 201
BbvI GCAGC 2 cut(s) 337, 1003
BccI CCATC 6 cut(s) 44, 151, 694, 740, 923, 1162
BceAI ACGGC 4 cut(s) 465, 493, 641, 703
BciT130I CCWGG 4 cut(s) 407, 434, 606, 851
BciVI GTATCC 2 cut(s) 202, 1117
BcoDI GTCTC 2 cut(s) 275, 495
BfaI CTAG 2 cut(s) 1184, 1284
BfmI CTRYAG 2 cut(s) 262, 379
BfuI GTATCC 2 cut(s) 202, 1117
BisI GCNGC 3 cut(s) 326, 936, 1017
BlsI GCNGC 3 cut(s) 327, 937, 1018
Bme1390I CCNGG 4 cut(s) 407, 434, 606, 851
Bme18I GGWCC 1 cut(s) 409
BmgT120I GGNCC 2 cut(s) 409, 1043
BmiI GGNNCC 7 cut(s) 188, 308, 411, 459, 848, 897, 1044
BmrFI CCNGG 4 cut(s) 407, 434, 606, 851
BplI GAGNNNNNCTC 4 cut(s) 185, 217, 522, 554
BpmI CTGGAG 1 cut(s) 127
Bsa29I ATCGAT 1 cut(s) 589
BsaBI GATNNNNATC 3 cut(s) 135, 585, 1119
BsaI GGTCTC 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 82, 432
BsaXI ACNNNNNCTCC 6 cut(s) 401, 431, 695, 725, 825, 855
Bsc4I CCNNNNNNNGG 1 cut(s) 267
Bse118I RCCGGY 1 cut(s) 291
Bse1I ACTGG 1 cut(s) 144
Bse3DI GCAATG 2 cut(s) 359, 773
Bse8I GATNNNNATC 3 cut(s) 135, 585, 1119
BseBI CCWGG 4 cut(s) 407, 434, 606, 851
BseCI ATCGAT 1 cut(s) 589
BseDI CCNNGG 2 cut(s) 82, 432
BseGI GGATG 2 cut(s) 347, 705
BseJI GATNNNNATC 3 cut(s) 135, 585, 1119
BseLI CCNNNNNNNGG 1 cut(s) 267
BseMI GCAATG 2 cut(s) 359, 773
BseMII CTCAG 2 cut(s) 234, 650
BseNI ACTGG 1 cut(s) 144
BseRI GAGGAG 3 cut(s) 13, 209, 1159
BseSI GKGCMC 1 cut(s) 1086
BseX3I CGGCCG 1 cut(s) 478
BseXI GCAGC 2 cut(s) 337, 1003
BseYI CCCAGC 1 cut(s) 412
BsgI GTGCAG 1 cut(s) 782
Bsh1236I CGCG 1 cut(s) 129
Bsh1285I CGRYCG 1 cut(s) 481
BshFI GGCC 4 cut(s) 295, 480, 938, 1045
BshNI GGYRCC 3 cut(s) 186, 846, 895
BshVI ATCGAT 1 cut(s) 589
BsiEI CGRYCG 1 cut(s) 481
BsiHKAI GWGCWC 1 cut(s) 201
BsiSI CCGG 1 cut(s) 292
BslFI GGGAC 1 cut(s) 874
BslI CCNNNNNNNGG 1 cut(s) 267
BsmAI GTCTC 2 cut(s) 275, 495
BsmFI GGGAC 1 cut(s) 874
BsmI GAATGC 1 cut(s) 1194
BsnI GGCC 4 cut(s) 295, 480, 938, 1045
Bso31I GGTCTC 1 cut(s) 495
Bsp1286I GDGCHC 2 cut(s) 201, 1086
Bsp143I GATC 6 cut(s) 130, 246, 306, 535, 586, 667
Bsp19I CCATGG 1 cut(s) 82
BspACI CCGC 2 cut(s) 127, 935
BspANI GGCC 4 cut(s) 295, 480, 938, 1045
BspCNI CTCAG 2 cut(s) 235, 649
BspDI ATCGAT 1 cut(s) 589
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 7 cut(s) 188, 308, 411, 459, 848, 897, 1044
BspPI GGATC 3 cut(s) 301, 314, 662
BspQI GCTCTTC 1 cut(s) 1223
BspT107I GGYRCC 3 cut(s) 186, 846, 895
BspTNI GGTCTC 1 cut(s) 495
BsrBI CCGCTC 1 cut(s) 935
BsrDI GCAATG 2 cut(s) 359, 773
BsrFI RCCGGY 1 cut(s) 291
BsrI ACTGG 1 cut(s) 144
BssAI RCCGGY 1 cut(s) 291
BssECI CCNNGG 2 cut(s) 82, 432
BssMI GATC 6 cut(s) 130, 246, 306, 535, 586, 667
BssT1I CCWWGG 1 cut(s) 82
Bst2UI CCWGG 4 cut(s) 407, 434, 606, 851
Bst4CI ACNGT 3 cut(s) 393, 428, 545
Bst6I CTCTTC 1 cut(s) 1223
BstC8I GCNNGC 2 cut(s) 357, 626
BstDEI CTNAG 4 cut(s) 243, 314, 421, 636
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 2 cut(s) 347, 705
BstFNI CGCG 1 cut(s) 129
BstKTI GATC 6 cut(s) 133, 249, 309, 538, 589, 670
BstMAI GTCTC 2 cut(s) 275, 495
BstMBI GATC 6 cut(s) 130, 246, 306, 535, 586, 667
BstMCI CGRYCG 1 cut(s) 481
BstMWI GCNNNNNNNGC 3 cut(s) 248, 361, 1051
BstNI CCWGG 4 cut(s) 407, 434, 606, 851
BstSCI CCNGG 4 cut(s) 405, 432, 604, 849
BstSFI CTRYAG 2 cut(s) 262, 379
BstSLI GKGCMC 1 cut(s) 1086
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 2 cut(s) 337, 1003
BstX2I RGATCY 1 cut(s) 306
BstXI CCANNNNNNTGG 1 cut(s) 678
BstYI RGATCY 1 cut(s) 306
BstZI CGGCCG 1 cut(s) 478
Bsu15I ATCGAT 1 cut(s) 589
BsuI GTATCC 2 cut(s) 202, 1117
BsuRI GGCC 4 cut(s) 295, 480, 938, 1045
BsuTUI ATCGAT 1 cut(s) 589
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 2 cut(s) 347, 705
BtsI GCAGTG 1 cut(s) 799
BtsIMutI CAGTG 2 cut(s) 151, 799
Cac8I GCNNGC 2 cut(s) 357, 626
CaiI CAGNNNCTG 1 cut(s) 869
Cfr10I RCCGGY 1 cut(s) 291
Cfr13I GGNCC 2 cut(s) 409, 1043
ClaI ATCGAT 1 cut(s) 589
Csp6I GTAC 2 cut(s) 429, 847
CviAII CATG 5 cut(s) 83, 329, 817, 857, 1249
CviQI GTAC 2 cut(s) 429, 847
DdeI CTNAG 4 cut(s) 243, 314, 421, 636
DpnI GATC 6 cut(s) 132, 248, 308, 537, 588, 669
DpnII GATC 6 cut(s) 130, 246, 306, 535, 586, 667
DraI TTTAAA 1 cut(s) 1036
EaeI YGGCCR 2 cut(s) 293, 478
EagI CGGCCG 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 1223
EarI CTCTTC 1 cut(s) 1223
Ecl136II GAGCTC 1 cut(s) 199
EclXI CGGCCG 1 cut(s) 478
Eco130I CCWWGG 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 201
Eco31I GGTCTC 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 409
Eco52I CGGCCG 1 cut(s) 478
Eco53kI GAGCTC 1 cut(s) 199
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 4 cut(s) 405, 432, 604, 849
EcoT14I CCWWGG 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 82
FaeI CATG 5 cut(s) 86, 332, 820, 860, 1252
FaqI GGGAC 1 cut(s) 874
FatI CATG 5 cut(s) 82, 328, 816, 856, 1248
Fnu4HI GCNGC 3 cut(s) 326, 936, 1017
FokI GGATG 2 cut(s) 334, 712
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 3 cut(s) 326, 936, 1017
FspBI CTAG 2 cut(s) 1184, 1284
GluI GCNGC 3 cut(s) 326, 936, 1017
GsaI CCCAGC 1 cut(s) 416
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 4 cut(s) 295, 480, 938, 1045
HapII CCGG 1 cut(s) 292
Hin1II CATG 5 cut(s) 86, 332, 820, 860, 1252
HindIII AAGCTT 1 cut(s) 66
HinfI GANTC 2 cut(s) 366, 599
HpaII CCGG 1 cut(s) 292
HphI GGTGA 4 cut(s) 91, 106, 901, 1070
Hpy166II GTNNAC 1 cut(s) 485
Hpy188I TCNGA 5 cut(s) 270, 306, 352, 639, 1313
Hpy188III TCNNGA 7 cut(s) 278, 445, 679, 945, 1124, 1141, 1260
Hpy8I GTNNAC 1 cut(s) 485
HpyAV CCTTC 7 cut(s) 23, 117, 320, 327, 571, 997, 1121
HpyCH4III ACNGT 3 cut(s) 393, 428, 545
HpyCH4V TGCA 6 cut(s) 359, 799, 816, 1019, 1192, 1307
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 361, 1051
HpyF3I CTNAG 4 cut(s) 243, 314, 421, 636
Hsp92II CATG 5 cut(s) 86, 332, 820, 860, 1252
KpnI GGTACC 1 cut(s) 850
Kzo9I GATC 6 cut(s) 130, 246, 306, 535, 586, 667
LguI GCTCTTC 1 cut(s) 1223
LmnI GCTCC 5 cut(s) 196, 337, 552, 619, 932
Lsp1109I GCAGC 2 cut(s) 337, 1003
MaeI CTAG 2 cut(s) 1184, 1284
MaeIII GTNAC 3 cut(s) 60, 256, 982
MalI GATC 6 cut(s) 132, 248, 308, 537, 588, 669
MbiI CCGCTC 1 cut(s) 935
MboI GATC 6 cut(s) 130, 246, 306, 535, 586, 667
MboII GAAGA 6 cut(s) 173, 528, 794, 1177, 1210, 1221
MflI RGATCY 1 cut(s) 306
MhlI GDGCHC 2 cut(s) 201, 1086
MluCI AATT 8 cut(s) 165, 213, 299, 547, 1029, 1037, 1063, 1263
MmeI TCCRAC 1 cut(s) 248
MroXI GAANNNNTTC 1 cut(s) 393
MseI TTAA 6 cut(s) 168, 216, 473, 684, 1035, 1241
MslI CAYNNNNRTG 1 cut(s) 144
MspI CCGG 1 cut(s) 292
MspR9I CCNGG 4 cut(s) 407, 434, 606, 851
Mva1269I GAATGC 1 cut(s) 1194
MvaI CCWGG 4 cut(s) 407, 434, 606, 851
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 3 cut(s) 248, 361, 1051
NcoI CCATGG 1 cut(s) 82
NdeII GATC 6 cut(s) 130, 246, 306, 535, 586, 667
NlaIII CATG 5 cut(s) 86, 332, 820, 860, 1252
NlaIV GGNNCC 7 cut(s) 188, 308, 411, 459, 848, 897, 1044
OliI CACNNNNGTG 1 cut(s) 144
PciSI GCTCTTC 1 cut(s) 1223
PctI GAATGC 1 cut(s) 1194
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 2 cut(s) 366, 599
PfoI TCCNGGA 1 cut(s) 604
PkrI GCNGC 3 cut(s) 327, 937, 1018
PshBI ATTAAT 1 cut(s) 684
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 4 cut(s) 405, 432, 604, 849
PspFI CCCAGC 1 cut(s) 412
PspGI CCWGG 4 cut(s) 405, 432, 604, 849
PspN4I GGNNCC 7 cut(s) 188, 308, 411, 459, 848, 897, 1044
PspPI GGNCC 2 cut(s) 409, 1043
PsrI GAACNNNNNNTAC 2 cut(s) 381, 413
PstNI CAGNNNCTG 1 cut(s) 869
PsuI RGATCY 1 cut(s) 306
RsaI GTAC 2 cut(s) 430, 848
RsaNI GTAC 2 cut(s) 429, 847
RseI CAYNNNNRTG 1 cut(s) 144
SacI GAGCTC 1 cut(s) 201
SapI GCTCTTC 1 cut(s) 1223
SaqAI TTAA 6 cut(s) 168, 216, 473, 684, 1035, 1241
SatI GCNGC 3 cut(s) 326, 936, 1017
Sau3AI GATC 6 cut(s) 130, 246, 306, 535, 586, 667
Sau96I GGNCC 2 cut(s) 409, 1043
ScrFI CCNGG 4 cut(s) 407, 434, 606, 851
SduI GDGCHC 2 cut(s) 201, 1086
SfcI CTRYAG 2 cut(s) 262, 379
SinI GGWCC 1 cut(s) 409
SmiMI CAYNNNNRTG 1 cut(s) 144
Sse9I AATT 8 cut(s) 165, 213, 299, 547, 1029, 1037, 1063, 1263
SsiI CCGC 2 cut(s) 127, 935
SspMI CTAG 2 cut(s) 1184, 1284
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 4 cut(s) 405, 432, 604, 849
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 3 cut(s) 393, 428, 545
TaqI TCGA 3 cut(s) 589, 882, 1140
TasI AATT 8 cut(s) 165, 213, 299, 547, 1029, 1037, 1063, 1263
TauI GCSGC 1 cut(s) 938
TfiI GAWTC 2 cut(s) 366, 599
Tru1I TTAA 6 cut(s) 168, 216, 473, 684, 1035, 1241
Tru9I TTAA 6 cut(s) 168, 216, 473, 684, 1035, 1241
TscAI CASTG 2 cut(s) 151, 806
TseI GCWGC 2 cut(s) 325, 1016
TspDTI ATGAA 3 cut(s) 782, 1020, 1237
TspGWI ACGGA 1 cut(s) 661
TspRI CASTG 2 cut(s) 151, 806
VpaK11BI GGWCC 1 cut(s) 409
VspI ATTAAT 1 cut(s) 684
XapI RAATTY 2 cut(s) 299, 1263
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 2 cut(s) 1184, 1284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.