Rh6DG442500

Zn_pept

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008016
Physical Location & Seq
Reverse (-)
61797544 .. 61801142
3599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG442500.1

Sequence Viewer

Length: 1305 bp
ATGGCTCATCAGCTTCCTCTATGGTCTCCCTTGTGCATCAGTTTGTGCTTCTTTGCTTGGTTGCAAGGTTTCTTCTCCGTCGTCCATGGCGATACTAATCTCTCTTACCCCTCTCTCACCCCCATTGACCGCGATCTCTACCATTCTAGTGGGGATTTGATGGAAGAAATTAAAGCTTTGGTCCTTCGACACAAAGATAAACTCACTTTGGATACAATTAAGGCTCAAAACAAGGGTTACTCTGCTGAGATTGCTGTAGTTACTTATAGTCGGAGAAGGCAGGAGGCCGATGACAAGCCAAGGTTTCGGATCCTTCTTAGTTTCGGGCAGCATGGAAGGGAGCTCATTACATCTGAAGTCGCTTTGCGAATCCTTTCAATCTTGTGTATAGAAAAGTTTCTACCCAACCTAGACCAAGCTTCCTTGGACCAGACCCTTGACAATCTTGTGATAAAGGTGGTGCCCATGGAAAACCTAAATGGCCGCAGACTTGTTGAGGCAGGAGACCTCTGCGAGAGGAGAAATGGGAGAGGAGTTGATCTTAACCGTAATTGGAGCGTAGATTGGGGCAAAAAAGAGAAGGACTATGATCCGTATGAGGAAAATCCTGGGACTGCTCCTTTCAGTGAGCCTGAAGCTCAAATAATGCGGAAAATTGCAGTGTCATTTGATCCACACATATGGGTTAATATACACTCTGGGATGGAGGCTCTATTTATGCCGTACGACCATAAGAACACAACCCCTGATGGAGTTCCCTCACAAAAGATGAAGTCATTGCTTGAAGAACTGAACCGTCTGCATTGCCAAAAACGTTGCATGATTGGGTCTGGTGGAGGTTCTGTTGGATATCTGGCACATGGGACAGCAACTGATTTTATGTTTGACATTGTAAAGGTGCCAATGGCTTTCACCTTTGAGATATATGGAGATGGAGCAGCCTCATCAAAAGATTGCTTTAAAATGTTCAATCCCACTGACTTCGGTACCTTCAATAGGGTTCTCAATGAATGGTCAGCTGCATTTTTCACAATTTTTAAATTGGGGCCAAACCTGCTCGGTGAAAATTATTCAAAGCCCCCCTTAGACAAGTGGGTGTCCATAGATGAGTATCTTGATGGGTACTTGGTCGAGAGGAGTAGTAGATATGGAAAGAAGATGGAGGTGCTTGAGCTAGGACTGCAGGAGATAAGAACATATTTCAGGCTCTTCTTGTTATCCTCAGTTCTGTTACTGTTCATGTTCTGTTCCAGAATTTCAAAAAGCAAGTTTTCTAGACCAATTGTTTCTGCTATTGCACTCTGA

Protein Analysis

434

Amino Acids

49.4

Weight (kDa)

6.57

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M14 PF00246 57 - 321 4.1e-27 Zinc carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1062
Acc65I GGTACC 1 cut(s) 986
AccB1I GGYRCC 3 cut(s) 460, 898, 986
AccII CGCG 1 cut(s) 132
AciI CCGC 3 cut(s) 130, 484, 649
AclI AACGTT 1 cut(s) 814
AclWI GGATC 4 cut(s) 304, 317, 584, 665
AcoI YGGCCR 1 cut(s) 481
AcsI RAATTY 1 cut(s) 1254
AcuI CTGAAG 2 cut(s) 375, 654
AfaI GTAC 3 cut(s) 725, 988, 1124
AfiI CCNNNNNNNGG 1 cut(s) 996
AgsI TTSAA 6 cut(s) 378, 785, 970, 994, 1074, 1260
AjnI CCWGG 1 cut(s) 607
AluBI AGCT 7 cut(s) 13, 176, 343, 419, 638, 1019, 1174
AluI AGCT 7 cut(s) 13, 176, 343, 419, 638, 1019, 1174
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 2 cut(s) 30, 498
AlwI GGATC 4 cut(s) 304, 317, 584, 665
AlwNI CAGNNNCTG 1 cut(s) 872
AoxI GGCC 3 cut(s) 285, 481, 1046
ApeKI GCWGC 3 cut(s) 328, 938, 1019
ApoI RAATTY 1 cut(s) 1254
Asp700I GAANNNNTTC 2 cut(s) 373, 396
Asp718I GGTACC 1 cut(s) 986
AspS9I GGNCC 3 cut(s) 181, 427, 1046
AsuHPI GGTGA 3 cut(s) 109, 904, 1073
AvaII GGWCC 2 cut(s) 181, 427
BaeGI GKGCMC 1 cut(s) 465
BaeI ACNNNNGTAYC 2 cut(s) 970, 1003
BamHI GGATCC 1 cut(s) 309
BanI GGYRCC 3 cut(s) 460, 898, 986
BanII GRGCYC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 3 cut(s) 340, 950, 1006
BccI CCATC 6 cut(s) 154, 697, 743, 926, 1112, 1153
BceAI ACGGC 1 cut(s) 706
BciT130I CCWGG 1 cut(s) 609
BciVI GTATCC 1 cut(s) 205
BcoDI GTCTC 2 cut(s) 30, 498
BfaI CTAG 4 cut(s) 147, 410, 1175, 1275
BfmI CTRYAG 2 cut(s) 255, 1181
BfuAI ACCTGC 1 cut(s) 1062
BfuI GTATCC 1 cut(s) 205
BisI GCNGC 4 cut(s) 329, 484, 939, 1020
BlsI GCNGC 4 cut(s) 330, 485, 940, 1021
Bme1390I CCNGG 1 cut(s) 609
Bme18I GGWCC 2 cut(s) 181, 427
BmgT120I GGNCC 3 cut(s) 181, 427, 1046
BmiI GGNNCC 5 cut(s) 311, 462, 900, 988, 1047
BmrFI CCNGG 1 cut(s) 609
BmsI GCATC 1 cut(s) 45
BpuEI CTTGAG 1 cut(s) 1190
BsaBI GATNNNNATC 2 cut(s) 96, 1110
BsaI GGTCTC 2 cut(s) 30, 498
BsaJI CCNNGG 5 cut(s) 85, 299, 423, 465, 608
BsaXI ACNNNNNCTCC 2 cut(s) 828, 858
Bsc4I CCNNNNNNNGG 1 cut(s) 996
Bse3DI GCAATG 2 cut(s) 776, 802
Bse8I GATNNNNATC 2 cut(s) 96, 1110
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 5 cut(s) 85, 299, 423, 465, 608
BseGI GGATG 1 cut(s) 708
BseJI GATNNNNATC 2 cut(s) 96, 1110
BseLI CCNNNNNNNGG 1 cut(s) 996
BseMI GCAATG 2 cut(s) 776, 802
BseMII CTCAG 2 cut(s) 237, 1236
BseRI GAGGAG 3 cut(s) 532, 546, 1150
BseSI GKGCMC 1 cut(s) 465
BseXI GCAGC 3 cut(s) 340, 950, 1006
Bsh1236I CGCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 287, 483, 1048
BshNI GGYRCC 3 cut(s) 460, 898, 986
BsiHKAI GWGCWC 1 cut(s) 345
BsiWI CGTACG 1 cut(s) 723
BslFI GGGAC 2 cut(s) 625, 877
BslI CCNNNNNNNGG 1 cut(s) 996
BsmAI GTCTC 2 cut(s) 30, 498
BsmFI GGGAC 2 cut(s) 625, 877
BsnI GGCC 3 cut(s) 287, 483, 1048
Bso31I GGTCTC 2 cut(s) 30, 498
Bsp1286I GDGCHC 2 cut(s) 345, 465
Bsp143I GATC 5 cut(s) 133, 309, 538, 589, 670
Bsp19I CCATGG 2 cut(s) 85, 465
BspACI CCGC 3 cut(s) 130, 484, 649
BspANI GGCC 3 cut(s) 287, 483, 1048
BspCNI CTCAG 2 cut(s) 238, 1235
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 5 cut(s) 311, 462, 900, 988, 1047
BspMAI CTGCAG 1 cut(s) 1185
BspMI ACCTGC 1 cut(s) 1062
BspPI GGATC 4 cut(s) 304, 317, 584, 665
BspQI GCTCTTC 1 cut(s) 1214
BspT107I GGYRCC 3 cut(s) 460, 898, 986
BspTNI GGTCTC 2 cut(s) 30, 498
BsrDI GCAATG 2 cut(s) 776, 802
BssECI CCNNGG 5 cut(s) 85, 299, 423, 465, 608
BssMI GATC 5 cut(s) 133, 309, 538, 589, 670
BssT1I CCWWGG 4 cut(s) 85, 299, 423, 465
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 3 cut(s) 548, 797, 1236
Bst6I CTCTTC 1 cut(s) 1214
BstDEI CTNAG 4 cut(s) 246, 317, 1084, 1222
BstDSI CCRYGG 2 cut(s) 85, 465
BstENI CCTNNNNNAGG 1 cut(s) 994
BstF5I GGATG 1 cut(s) 708
BstFNI CGCG 1 cut(s) 132
BstKTI GATC 5 cut(s) 136, 312, 541, 592, 673
BstMAI GTCTC 2 cut(s) 30, 498
BstMBI GATC 5 cut(s) 133, 309, 538, 589, 670
BstMWI GCNNNNNNNGC 3 cut(s) 251, 1054, 1180
BstNI CCWGG 1 cut(s) 609
BstSCI CCNGG 1 cut(s) 607
BstSFI CTRYAG 2 cut(s) 255, 1181
BstSLI GKGCMC 1 cut(s) 465
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 3 cut(s) 340, 950, 1006
BstX2I RGATCY 1 cut(s) 309
BstXI CCANNNNNNTGG 2 cut(s) 149, 681
BstYI RGATCY 1 cut(s) 309
BsuI GTATCC 1 cut(s) 205
BsuRI GGCC 3 cut(s) 287, 483, 1048
BtgI CCRYGG 2 cut(s) 85, 465
BtsCI GGATG 1 cut(s) 708
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 3 cut(s) 631, 666, 975
BveI ACCTGC 1 cut(s) 1062
CaiI CAGNNNCTG 1 cut(s) 872
Cfr13I GGNCC 3 cut(s) 181, 427, 1046
Csp6I GTAC 3 cut(s) 724, 987, 1123
CviAII CATG 6 cut(s) 86, 332, 466, 820, 860, 1240
CviQI GTAC 3 cut(s) 724, 987, 1123
DdeI CTNAG 4 cut(s) 246, 317, 1084, 1222
DpnI GATC 5 cut(s) 135, 311, 540, 591, 672
DpnII GATC 5 cut(s) 133, 309, 538, 589, 670
DraI TTTAAA 2 cut(s) 961, 1039
EaeI YGGCCR 1 cut(s) 481
Eam1104I CTCTTC 1 cut(s) 1214
EarI CTCTTC 1 cut(s) 1214
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 4 cut(s) 85, 299, 423, 465
Eco24I GRGCYC 1 cut(s) 345
Eco31I GGTCTC 2 cut(s) 30, 498
Eco32I GATATC 1 cut(s) 851
Eco47I GGWCC 2 cut(s) 181, 427
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 2 cut(s) 375, 654
EcoICRI GAGCTC 1 cut(s) 343
EcoNI CCTNNNNNAGG 1 cut(s) 994
EcoRII CCWGG 1 cut(s) 607
EcoRV GATATC 1 cut(s) 851
EcoT14I CCWWGG 4 cut(s) 85, 299, 423, 465
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 4 cut(s) 85, 299, 423, 465
FaeI CATG 6 cut(s) 89, 335, 469, 823, 863, 1243
FalI AAGNNNNNCTT 2 cut(s) 1067, 1099
FaqI GGGAC 2 cut(s) 625, 877
FatI CATG 6 cut(s) 85, 331, 465, 819, 859, 1239
FauNDI CATATG 1 cut(s) 680
Fnu4HI GCNGC 4 cut(s) 329, 484, 939, 1020
FokI GGATG 1 cut(s) 715
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 4 cut(s) 329, 484, 939, 1020
FspBI CTAG 4 cut(s) 147, 410, 1175, 1275
GluI GCNGC 4 cut(s) 329, 484, 939, 1020
HaeIII GGCC 3 cut(s) 287, 483, 1048
Hin1II CATG 6 cut(s) 89, 335, 469, 823, 863, 1243
HindIII AAGCTT 2 cut(s) 174, 417
HinfI GANTC 1 cut(s) 369
HphI GGTGA 3 cut(s) 109, 904, 1073
Hpy188I TCNGA 4 cut(s) 273, 309, 355, 1304
Hpy188III TCNNGA 4 cut(s) 1115, 1132, 1251, 1275
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 6 cut(s) 194, 270, 323, 330, 574, 1000
HpyCH4III ACNGT 3 cut(s) 548, 797, 1236
HpyCH4IV ACGT 1 cut(s) 814
HpyCH4V TGCA 8 cut(s) 36, 64, 659, 802, 819, 1022, 1183, 1298
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 1054, 1180
HpyF3I CTNAG 4 cut(s) 246, 317, 1084, 1222
HpySE526I ACGT 1 cut(s) 814
Hsp92II CATG 6 cut(s) 89, 335, 469, 823, 863, 1243
KpnI GGTACC 1 cut(s) 990
Kzo9I GATC 5 cut(s) 133, 309, 538, 589, 670
LguI GCTCTTC 1 cut(s) 1214
LmnI GCTCC 4 cut(s) 340, 555, 622, 935
Lsp1109I GCAGC 3 cut(s) 340, 950, 1006
LweI GCATC 1 cut(s) 45
MaeI CTAG 4 cut(s) 147, 410, 1175, 1275
MaeII ACGT 1 cut(s) 814
MaeIII GTNAC 3 cut(s) 236, 259, 1230
MalI GATC 5 cut(s) 135, 311, 540, 591, 672
MboI GATC 5 cut(s) 133, 309, 538, 589, 670
MboII GAAGA 5 cut(s) 64, 176, 797, 1168, 1201
MfeI CAATTG 1 cut(s) 1281
MflI RGATCY 1 cut(s) 309
MhlI GDGCHC 2 cut(s) 345, 465
MluCI AATT 9 cut(s) 168, 216, 550, 654, 1032, 1040, 1066, 1254, 1281
MmeI TCCRAC 2 cut(s) 251, 826
MroXI GAANNNNTTC 2 cut(s) 373, 396
MseI TTAA 6 cut(s) 171, 219, 543, 687, 960, 1038
MslI CAYNNNNRTG 2 cut(s) 147, 679
MspA1I CMGCKG 1 cut(s) 1019
MspR9I CCNGG 1 cut(s) 609
MunI CAATTG 1 cut(s) 1281
MvaI CCWGG 1 cut(s) 609
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 3 cut(s) 251, 1054, 1180
NcoI CCATGG 2 cut(s) 85, 465
NdeI CATATG 1 cut(s) 680
NdeII GATC 5 cut(s) 133, 309, 538, 589, 670
NlaIII CATG 6 cut(s) 89, 335, 469, 823, 863, 1243
NlaIV GGNNCC 5 cut(s) 311, 462, 900, 988, 1047
PciSI GCTCTTC 1 cut(s) 1214
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PdmI GAANNNNTTC 2 cut(s) 373, 396
PfeI GAWTC 1 cut(s) 369
Pfl23II CGTACG 1 cut(s) 723
PkrI GCNGC 4 cut(s) 330, 485, 940, 1021
Psp124BI GAGCTC 1 cut(s) 345
Psp1406I AACGTT 1 cut(s) 814
Psp6I CCWGG 1 cut(s) 607
PspGI CCWGG 1 cut(s) 607
PspLI CGTACG 1 cut(s) 723
PspN4I GGNNCC 5 cut(s) 311, 462, 900, 988, 1047
PspPI GGNCC 3 cut(s) 181, 427, 1046
PstI CTGCAG 1 cut(s) 1185
PstNI CAGNNNCTG 1 cut(s) 872
PsuI RGATCY 1 cut(s) 309
PvuII CAGCTG 1 cut(s) 1019
RsaI GTAC 3 cut(s) 725, 988, 1124
RsaNI GTAC 3 cut(s) 724, 987, 1123
RseI CAYNNNNRTG 2 cut(s) 147, 679
SacI GAGCTC 1 cut(s) 345
SapI GCTCTTC 1 cut(s) 1214
SaqAI TTAA 6 cut(s) 171, 219, 543, 687, 960, 1038
SatI GCNGC 4 cut(s) 329, 484, 939, 1020
Sau3AI GATC 5 cut(s) 133, 309, 538, 589, 670
Sau96I GGNCC 3 cut(s) 181, 427, 1046
ScrFI CCNGG 1 cut(s) 609
SduI GDGCHC 2 cut(s) 345, 465
SfaNI GCATC 1 cut(s) 45
SfcI CTRYAG 2 cut(s) 255, 1181
SinI GGWCC 2 cut(s) 181, 427
SmiMI CAYNNNNRTG 2 cut(s) 147, 679
SmlI CTYRAG 1 cut(s) 1169
SmoI CTYRAG 1 cut(s) 1169
Sse9I AATT 9 cut(s) 168, 216, 550, 654, 1032, 1040, 1066, 1254, 1281
SsiI CCGC 3 cut(s) 130, 484, 649
SspMI CTAG 4 cut(s) 147, 410, 1175, 1275
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 607
StyI CCWWGG 4 cut(s) 85, 299, 423, 465
TaaI ACNGT 3 cut(s) 548, 797, 1236
TaiI ACGT 1 cut(s) 817
TaqI TCGA 2 cut(s) 187, 1131
TasI AATT 9 cut(s) 168, 216, 550, 654, 1032, 1040, 1066, 1254, 1281
TauI GCSGC 1 cut(s) 486
TfiI GAWTC 1 cut(s) 369
Tru1I TTAA 6 cut(s) 171, 219, 543, 687, 960, 1038
Tru9I TTAA 6 cut(s) 171, 219, 543, 687, 960, 1038
TscAI CASTG 3 cut(s) 631, 666, 982
TseI GCWGC 3 cut(s) 328, 938, 1019
TspDTI ATGAA 3 cut(s) 785, 1023, 1228
TspGWI ACGGA 2 cut(s) 67, 582
TspRI CASTG 3 cut(s) 631, 666, 982
VpaK11BI GGWCC 2 cut(s) 181, 427
XagI CCTNNNNNAGG 1 cut(s) 994
XapI RAATTY 1 cut(s) 1254
XbaI TCTAGA 1 cut(s) 1274
XmnI GAANNNNTTC 2 cut(s) 373, 396
XspI CTAG 4 cut(s) 147, 410, 1175, 1275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.