MD08G1002400.v1.1

Zn_pept

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008016
Physical Location & Seq
Reverse (-)
260440 .. 264144
3705 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1002400.v1.1.491

Sequence Viewer

Length: 1374 bp
ATGAAAACCCAAACCATGGCTCATCTCCTTGTTCCTCCGATGATTGCCAATGCCATGGCTCATCTCCTTCCTCTCTCCTCTCCTTTATCCATCACTCTCTGCTTCTTTGCGTGGTTGCAAGCTTTCTTGGTCGTCCATGGCAATACTAATAACATCACCAATCCTTCTTCTCTCACCCCCATGAACCGCGATCTCTACCATTCCAGTGGGGATTTGATGGAAGAAATTAAAGCTCTCGTCTTTCGGCACCCAGACGAGCTCACTTTGGATACAATGAAATCTCGAAACAAGGGCTACTCTGCTGAGATCGCAATAGTTACCTATTGCCGGAGAAGGCAAGAGACTGATGACCAGCCAAAGTTACGGATCCTTCTTAGTTTTGGGCAGCATGGAAGGGAGCTTATTACATCCGAGCTTGCATTACGAATCCTATCAATCTTGAGTAAAGAACAGTTTCTACCCAACCTGGACCCCGTGTCCTTAGACAGTACCCTTGACAAGCTCTTGATAAAGGTGGTTCCGATGGAAAACTTAAATGGCCGTAGACTTGTTGAAGCAGGAGACCTCTGTGAGAGGAGAAATGGGAGGGGAGTTGATCCCAACCGTAATTGGAGCGTGGATTGGGGAAAAAAAGAGAAGGATTATGATCCATATGAGGAAAATCCTGGAACTGCTCCTTTCAGCGAGCCTGAAACTCAGATAATGCGGAAAGTTTCCATGTCATTTGATCCACACATATGGATTAATATACACTCTGGGATGGAGGCTCTATTTATGCCGTATGACCATAAGAACACAACCCCAGATGGACTTTCCTCCCACCAGATGAAGTCATTGCTTGAAGAACTCAACCAACTGCATTTCCAAAAGCGTTGCATGGTTGGGTCTGGTGGAGGTTCTGTTGGGTACCTGGCACATGGGACAGCAACTGATTTTATGTTCGACATTGTAAAGGTGCCAATGGCTTTCACCTTTGAGATATATGGAGATGGAGCAGCCTCATCAAGAGATTGCTTCAAAATGTTCAATCCCACAGAATTGGGTACCTTCAATAGAGTTCTCAATGAATGGTCAGCTGCATTTTTCACAATTTTTAAATTGGGGCCAGAGCAGCTTGGTGAAAATTATTCAAAGTCTTCTGTGCCCACCTTATTAGACAAGTGGGTATCCATAGATGAGTATCTTGAAGGGTACTTAGTCGAGAGGAGTAGTAGATACGGAAAGAAGATGGAGGTGCTTGAACTAGGAATGCAGGAGATAAGAACATATTTCAGGCTCTTCTTGTTATCGTCTGTTCTGTTAATGTTCATGTTCTGTTCCAGAATTTCAAAAAGCAAGTGTGCCAGACCAATTGTTTCTGCTATTGCAATCTGA

Protein Analysis

458

Amino Acids

51.87

Weight (kDa)

6.33

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M14 PF00246 75 - 344 1.2e-25 Zinc carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 906, 1043
AccB1I GGYRCC 4 cut(s) 246, 906, 955, 1043
AccB7I CCANNNNNTGG 1 cut(s) 16
AccI GTMKAC 1 cut(s) 544
AccII CGCG 1 cut(s) 189
AciI CCGC 2 cut(s) 187, 706
AclWI GGATC 5 cut(s) 361, 374, 590, 641, 722
AcoI YGGCCR 1 cut(s) 538
AcsI RAATTY 1 cut(s) 1323
AfaI GTAC 4 cut(s) 490, 908, 1045, 1193
AfiI CCNNNNNNNGG 2 cut(s) 16, 327
AgsI TTSAA 9 cut(s) 554, 842, 1018, 1027, 1051, 1131, 1187, 1241, 1329
AhdI GACNNNNNGTC 1 cut(s) 475
AjnI CCWGG 3 cut(s) 465, 664, 909
AluBI AGCT 8 cut(s) 122, 233, 259, 400, 415, 502, 1076, 1114
AluI AGCT 8 cut(s) 122, 233, 259, 400, 415, 502, 1076, 1114
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 2 cut(s) 335, 555
AlwI GGATC 5 cut(s) 361, 374, 590, 641, 722
AlwNI CAGNNNCTG 1 cut(s) 929
AoxI GGCC 2 cut(s) 538, 1103
ApeKI GCWGC 4 cut(s) 385, 995, 1076, 1111
ApoI RAATTY 1 cut(s) 1323
AseI ATTAAT 1 cut(s) 744
Asp700I GAANNNNTTC 1 cut(s) 453
Asp718I GGTACC 2 cut(s) 906, 1043
AspS9I GGNCC 2 cut(s) 469, 1103
AsuHPI GGTGA 4 cut(s) 148, 166, 961, 1130
AvaII GGWCC 1 cut(s) 469
BaeGI GKGCMC 1 cut(s) 1146
BamHI GGATCC 1 cut(s) 366
BanI GGYRCC 4 cut(s) 246, 906, 955, 1043
BanII GRGCYC 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 1128
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 4 cut(s) 397, 1007, 1063, 1123
BccI CCATC 7 cut(s) 98, 211, 517, 754, 800, 983, 1222
BceAI ACGGC 2 cut(s) 525, 763
BciT130I CCWGG 3 cut(s) 467, 666, 911
BciVI GTATCC 2 cut(s) 262, 1177
BcoDI GTCTC 2 cut(s) 335, 555
BfaI CTAG 1 cut(s) 1244
BfuI GTATCC 2 cut(s) 262, 1177
BisI GCNGC 4 cut(s) 386, 996, 1077, 1112
BlsI GCNGC 4 cut(s) 387, 997, 1078, 1113
Bme1390I CCNGG 3 cut(s) 467, 666, 911
Bme18I GGWCC 1 cut(s) 469
BmeRI GACNNNNNGTC 1 cut(s) 475
BmgT120I GGNCC 2 cut(s) 469, 1103
BmiI GGNNCC 8 cut(s) 248, 368, 471, 519, 908, 957, 1045, 1104
BmrFI CCNGG 3 cut(s) 467, 666, 911
BpiI GAAGAC 1 cut(s) 1128
BpuEI CTTGAG 1 cut(s) 460
BsaBI GATNNNNATC 2 cut(s) 645, 1179
BsaI GGTCTC 1 cut(s) 555
BsaJI CCNNGG 3 cut(s) 15, 54, 136
BsaXI ACNNNNNCTCC 2 cut(s) 885, 915
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 327
Bse1I ACTGG 1 cut(s) 204
Bse3DI GCAATG 1 cut(s) 833
Bse8I GATNNNNATC 2 cut(s) 645, 1179
BseBI CCWGG 3 cut(s) 467, 666, 911
BseDI CCNNGG 3 cut(s) 15, 54, 136
BseGI GGATG 2 cut(s) 407, 765
BseJI GATNNNNATC 2 cut(s) 645, 1179
BseLI CCNNNNNNNGG 2 cut(s) 16, 327
BseMI GCAATG 1 cut(s) 833
BseMII CTCAG 2 cut(s) 294, 710
BseNI ACTGG 1 cut(s) 204
BseRI GAGGAG 3 cut(s) 67, 589, 1219
BseSI GKGCMC 1 cut(s) 1146
BseXI GCAGC 4 cut(s) 397, 1007, 1063, 1123
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 2 cut(s) 540, 1105
BshNI GGYRCC 4 cut(s) 246, 906, 955, 1043
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 1 cut(s) 328
BslFI GGGAC 1 cut(s) 934
BslI CCNNNNNNNGG 2 cut(s) 16, 327
BsmAI GTCTC 2 cut(s) 335, 555
BsmFI GGGAC 1 cut(s) 934
BsmI GAATGC 1 cut(s) 1254
BsnI GGCC 2 cut(s) 540, 1105
Bso31I GGTCTC 1 cut(s) 555
Bsp1286I GDGCHC 2 cut(s) 261, 1146
Bsp143I GATC 6 cut(s) 190, 306, 366, 595, 646, 727
Bsp19I CCATGG 3 cut(s) 15, 54, 136
BspACI CCGC 2 cut(s) 187, 706
BspANI GGCC 2 cut(s) 540, 1105
BspCNI CTCAG 2 cut(s) 295, 709
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 8 cut(s) 248, 368, 471, 519, 908, 957, 1045, 1104
BspPI GGATC 5 cut(s) 361, 374, 590, 641, 722
BspQI GCTCTTC 1 cut(s) 1283
BspT107I GGYRCC 4 cut(s) 246, 906, 955, 1043
BspTNI GGTCTC 1 cut(s) 555
BsrDI GCAATG 1 cut(s) 833
BsrI ACTGG 1 cut(s) 204
BssECI CCNNGG 3 cut(s) 15, 54, 136
BssMI GATC 6 cut(s) 190, 306, 366, 595, 646, 727
BssT1I CCWWGG 3 cut(s) 15, 54, 136
Bst2UI CCWGG 3 cut(s) 467, 666, 911
Bst4CI ACNGT 3 cut(s) 453, 488, 605
Bst6I CTCTTC 1 cut(s) 1283
BstC8I GCNNGC 3 cut(s) 120, 417, 686
BstDEI CTNAG 5 cut(s) 303, 374, 481, 696, 1195
BstDSI CCRYGG 3 cut(s) 15, 54, 136
BstF5I GGATG 2 cut(s) 407, 765
BstFNI CGCG 1 cut(s) 189
BstKTI GATC 6 cut(s) 193, 309, 369, 598, 649, 730
BstMAI GTCTC 2 cut(s) 335, 555
BstMBI GATC 6 cut(s) 190, 306, 366, 595, 646, 727
BstMWI GCNNNNNNNGC 2 cut(s) 308, 1111
BstNI CCWGG 3 cut(s) 467, 666, 911
BstSCI CCNGG 3 cut(s) 465, 664, 909
BstSLI GKGCMC 1 cut(s) 1146
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 4 cut(s) 397, 1007, 1063, 1123
BstV2I GAAGAC 1 cut(s) 1128
BstX2I RGATCY 1 cut(s) 366
BstXI CCANNNNNNTGG 4 cut(s) 55, 206, 738, 1039
BstYI RGATCY 1 cut(s) 366
BsuI GTATCC 2 cut(s) 262, 1177
BsuRI GGCC 2 cut(s) 540, 1105
BtgI CCRYGG 3 cut(s) 15, 54, 136
BtsCI GGATG 2 cut(s) 407, 765
BtsIMutI CAGTG 1 cut(s) 211
Cac8I GCNNGC 3 cut(s) 120, 417, 686
CaiI CAGNNNCTG 1 cut(s) 929
Cfr13I GGNCC 2 cut(s) 469, 1103
Csp6I GTAC 4 cut(s) 489, 907, 1044, 1192
CviAII CATG 9 cut(s) 16, 55, 137, 181, 389, 718, 877, 917, 1309
CviQI GTAC 4 cut(s) 489, 907, 1044, 1192
DdeI CTNAG 5 cut(s) 303, 374, 481, 696, 1195
DpnI GATC 6 cut(s) 192, 308, 368, 597, 648, 729
DpnII GATC 6 cut(s) 190, 306, 366, 595, 646, 727
DraI TTTAAA 1 cut(s) 1096
DriI GACNNNNNGTC 1 cut(s) 475
EaeI YGGCCR 1 cut(s) 538
Eam1104I CTCTTC 1 cut(s) 1283
Eam1105I GACNNNNNGTC 1 cut(s) 475
EarI CTCTTC 1 cut(s) 1283
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 3 cut(s) 15, 54, 136
Eco24I GRGCYC 1 cut(s) 261
Eco31I GGTCTC 1 cut(s) 555
Eco47I GGWCC 1 cut(s) 469
Eco53kI GAGCTC 1 cut(s) 259
EcoICRI GAGCTC 1 cut(s) 259
EcoRII CCWGG 3 cut(s) 465, 664, 909
EcoT14I CCWWGG 3 cut(s) 15, 54, 136
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 3 cut(s) 15, 54, 136
FaeI CATG 9 cut(s) 19, 58, 140, 184, 392, 721, 880, 920, 1312
FaqI GGGAC 1 cut(s) 934
FatI CATG 9 cut(s) 15, 54, 136, 180, 388, 717, 876, 916, 1308
FauNDI CATATG 2 cut(s) 652, 737
FblI GTMKAC 1 cut(s) 544
Fnu4HI GCNGC 4 cut(s) 386, 996, 1077, 1112
FokI GGATG 2 cut(s) 394, 772
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 4 cut(s) 386, 996, 1077, 1112
FspBI CTAG 1 cut(s) 1244
GluI GCNGC 4 cut(s) 386, 996, 1077, 1112
HaeIII GGCC 2 cut(s) 540, 1105
HapII CCGG 1 cut(s) 328
Hin1II CATG 9 cut(s) 19, 58, 140, 184, 392, 721, 880, 920, 1312
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 1 cut(s) 426
HpaII CCGG 1 cut(s) 328
HphI GGTGA 4 cut(s) 148, 166, 961, 1130
Hpy166II GTNNAC 1 cut(s) 545
Hpy188I TCNGA 5 cut(s) 39, 412, 522, 699, 1373
Hpy188III TCNNGA 7 cut(s) 282, 439, 505, 1005, 1184, 1201, 1320
Hpy8I GTNNAC 1 cut(s) 545
HpyAV CCTTC 8 cut(s) 77, 174, 327, 380, 387, 631, 1057, 1181
HpyCH4III ACNGT 3 cut(s) 453, 488, 605
HpyCH4V TGCA 7 cut(s) 118, 419, 859, 876, 1079, 1252, 1367
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 1111
HpyF3I CTNAG 5 cut(s) 303, 374, 481, 696, 1195
Hsp92II CATG 9 cut(s) 19, 58, 140, 184, 392, 721, 880, 920, 1312
KpnI GGTACC 2 cut(s) 910, 1047
Kzo9I GATC 6 cut(s) 190, 306, 366, 595, 646, 727
LguI GCTCTTC 1 cut(s) 1283
LmnI GCTCC 4 cut(s) 397, 612, 679, 992
Lsp1109I GCAGC 4 cut(s) 397, 1007, 1063, 1123
MaeI CTAG 1 cut(s) 1244
MaeIII GTNAC 2 cut(s) 316, 360
MalI GATC 6 cut(s) 192, 308, 368, 597, 648, 729
MboI GATC 6 cut(s) 190, 306, 366, 595, 646, 727
MboII GAAGA 6 cut(s) 159, 233, 854, 1128, 1237, 1270
MfeI CAATTG 1 cut(s) 1350
MflI RGATCY 1 cut(s) 366
MhlI GDGCHC 2 cut(s) 261, 1146
MluCI AATT 8 cut(s) 225, 607, 1037, 1089, 1097, 1123, 1323, 1350
MroXI GAANNNNTTC 1 cut(s) 453
MseI TTAA 5 cut(s) 228, 533, 744, 1095, 1301
MslI CAYNNNNRTG 3 cut(s) 179, 204, 736
MspA1I CMGCKG 1 cut(s) 1076
MspI CCGG 1 cut(s) 328
MspR9I CCNGG 3 cut(s) 467, 666, 911
MunI CAATTG 1 cut(s) 1350
Mva1269I GAATGC 1 cut(s) 1254
MvaI CCWGG 3 cut(s) 467, 666, 911
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 2 cut(s) 308, 1111
NcoI CCATGG 3 cut(s) 15, 54, 136
NdeI CATATG 2 cut(s) 652, 737
NdeII GATC 6 cut(s) 190, 306, 366, 595, 646, 727
NlaIII CATG 9 cut(s) 19, 58, 140, 184, 392, 721, 880, 920, 1312
NlaIV GGNNCC 8 cut(s) 248, 368, 471, 519, 908, 957, 1045, 1104
PciSI GCTCTTC 1 cut(s) 1283
PctI GAATGC 1 cut(s) 1254
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 1 cut(s) 426
PflMI CCANNNNNTGG 1 cut(s) 16
PfoI TCCNGGA 1 cut(s) 664
PkrI GCNGC 4 cut(s) 387, 997, 1078, 1113
PshBI ATTAAT 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 261
Psp6I CCWGG 3 cut(s) 465, 664, 909
PspGI CCWGG 3 cut(s) 465, 664, 909
PspN4I GGNNCC 8 cut(s) 248, 368, 471, 519, 908, 957, 1045, 1104
PspPI GGNCC 2 cut(s) 469, 1103
PsrI GAACNNNNNNTAC 2 cut(s) 441, 473
PstNI CAGNNNCTG 1 cut(s) 929
PsuI RGATCY 1 cut(s) 366
PvuII CAGCTG 1 cut(s) 1076
RsaI GTAC 4 cut(s) 490, 908, 1045, 1193
RsaNI GTAC 4 cut(s) 489, 907, 1044, 1192
RseI CAYNNNNRTG 3 cut(s) 179, 204, 736
SacI GAGCTC 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 1283
SaqAI TTAA 5 cut(s) 228, 533, 744, 1095, 1301
SatI GCNGC 4 cut(s) 386, 996, 1077, 1112
Sau3AI GATC 6 cut(s) 190, 306, 366, 595, 646, 727
Sau96I GGNCC 2 cut(s) 469, 1103
ScrFI CCNGG 3 cut(s) 467, 666, 911
SduI GDGCHC 2 cut(s) 261, 1146
SinI GGWCC 1 cut(s) 469
SmiMI CAYNNNNRTG 3 cut(s) 179, 204, 736
SmlI CTYRAG 1 cut(s) 439
SmoI CTYRAG 1 cut(s) 439
Sse9I AATT 8 cut(s) 225, 607, 1037, 1089, 1097, 1123, 1323, 1350
SsiI CCGC 2 cut(s) 187, 706
SspMI CTAG 1 cut(s) 1244
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 3 cut(s) 465, 664, 909
StyI CCWWGG 3 cut(s) 15, 54, 136
TaaI ACNGT 3 cut(s) 453, 488, 605
TaqI TCGA 3 cut(s) 283, 942, 1200
TasI AATT 8 cut(s) 225, 607, 1037, 1089, 1097, 1123, 1323, 1350
TfiI GAWTC 1 cut(s) 426
Tru1I TTAA 5 cut(s) 228, 533, 744, 1095, 1301
Tru9I TTAA 5 cut(s) 228, 533, 744, 1095, 1301
TscAI CASTG 1 cut(s) 211
TseI GCWGC 4 cut(s) 385, 995, 1076, 1111
TspDTI ATGAA 6 cut(s) 17, 197, 290, 842, 1080, 1297
TspGWI ACGGA 2 cut(s) 379, 1233
TspRI CASTG 1 cut(s) 211
Van91I CCANNNNNTGG 1 cut(s) 16
VpaK11BI GGWCC 1 cut(s) 469
VspI ATTAAT 1 cut(s) 744
XapI RAATTY 1 cut(s) 1323
XmiI GTMKAC 1 cut(s) 544
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 1 cut(s) 1244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.