Rh6BG459500

Zn_pept

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0008016
Physical Location & Seq
Forward (+)
67058854 .. 67062713
3860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG459500.1

Sequence Viewer

Length: 1338 bp
ATGGCTCATCAGCTTCCTCTATGGTCTCCCTTGTGCATCAGTTTGTGCTTCTTTGCTTGGTTGCAAGGTTTCTTCTCCGTCGTCCATGGCGATACTAATCTCTCTTACCCCTCTCTCACCCCCATTGACCGCGATCTCTACCATTCTAGTGGGGATTTGATGGAAGAAATTAAAGCTTTGGTCCTTCGACACAAAGATAAACTCACTTTGGATACAATTAAGGCTCAAAACAAGGGTTACTCTGCTGAGATTGCTGTAGTTACTTATAGTCGGAGAAGGCAGGAGGCCGATGACAAGCCAAGGTTTCGGATCCTTCTTAGTTTCGGGCAGCATGGAAGGGAGCTCATTACATCCGAAGTCGCTTTGCGAATCCTTTCAATCTTGTGTATAGAAAAGTTTCTGCCCAACCTAGACCAAGCTTCCTTAGACCAGACCCTTGACAATCTTGTGATAAAGGTGGTGCCCATGGAAAACCTAAATGGCCGCAGACTTGTTGAGGCAGGAGACCTCTGCGAGAGGAGAAATGGGAGAGGAGTTGATCTTAACCGTAATTGGAGCGTAGATTGGGGCAAAAAAGAGAAGGACTATGATCCGTATGAGGAAAATCCTGGGACTGCTCCTTTCAGTGAGCCTGAAGCTCAAATAATGCGGAAAATTGCAGTGTCATTTGATCCACACATATGGGTTAATATACACTCTGGGATGGAGGTGGTTCAAACAGACTTCCTTATATTCCCTCATGCTCTATTTATGCCGTACGACCATAAGAACACAACCCCTGATGGAGTTGCCTCACAAAAGATGAAGTCATTGCTTGAAGAACTGAACCGTCTGCATTGCCAAAAACGTTGCATGATTGGGTCTGGTGGAGGTTCTGTTGGATATCTGGCACATGGGACAGCAACTGATTTTATGTTTGACATTGTAAAGGTGCCAATGGCTTTCACCTTTGAGATATATGGAGATGGAGCAGCCTCATCAAAAGATTGCTTTAAAATGTTCAATCCCACTGACTTCGGTACCTTCAATAGGGTTCTCAATGAATGGTCAGCTGCATTTTTCACAATTTTTAAATTGGGGCCAAACCTGCTCGGTGAAAATTATTCAAAGCCCCCCTTAGACAAGTGGGTGTCCATAGATGAGTATCTTGATGGGTACTTGGTCGAGAGGAGTAGTAGATATGGAAAGAAGATGGAGGTGCTTGAGCTAGGACTGCAGGAGATAAGAACATATTTCAGGCTCTTCTTGTTATCCTCAGTTCTGTTACTGTTCATGTTCTGTTCCAGAATTTCAAAAAGCAAGTTTTCTAGACCAATTGTTTCTGCTATTGCACTCTGA

Protein Analysis

445

Amino Acids

50.67

Weight (kDa)

6.43

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M14 PF00246 57 - 332 7.6e-24 Zinc carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1095
Acc65I GGTACC 1 cut(s) 1019
AccB1I GGYRCC 3 cut(s) 460, 931, 1019
AccII CGCG 1 cut(s) 132
AciI CCGC 3 cut(s) 130, 484, 649
AclI AACGTT 1 cut(s) 847
AclWI GGATC 4 cut(s) 304, 317, 584, 665
AcoI YGGCCR 1 cut(s) 481
AcsI RAATTY 1 cut(s) 1287
AcuI CTGAAG 1 cut(s) 654
AfaI GTAC 3 cut(s) 758, 1021, 1157
AfiI CCNNNNNNNGG 1 cut(s) 1029
AgsI TTSAA 7 cut(s) 378, 716, 818, 1003, 1027, 1107, 1293
AjnI CCWGG 1 cut(s) 607
AluBI AGCT 7 cut(s) 13, 176, 343, 419, 638, 1052, 1207
AluI AGCT 7 cut(s) 13, 176, 343, 419, 638, 1052, 1207
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 2 cut(s) 30, 498
AlwI GGATC 4 cut(s) 304, 317, 584, 665
AlwNI CAGNNNCTG 1 cut(s) 905
AoxI GGCC 3 cut(s) 285, 481, 1079
ApeKI GCWGC 3 cut(s) 328, 971, 1052
ApoI RAATTY 1 cut(s) 1287
Asp700I GAANNNNTTC 2 cut(s) 373, 396
Asp718I GGTACC 1 cut(s) 1019
AspS9I GGNCC 2 cut(s) 181, 1079
AsuHPI GGTGA 3 cut(s) 109, 937, 1106
AvaII GGWCC 1 cut(s) 181
BaeGI GKGCMC 1 cut(s) 465
BaeI ACNNNNGTAYC 2 cut(s) 1003, 1036
BamHI GGATCC 1 cut(s) 309
BanI GGYRCC 3 cut(s) 460, 931, 1019
BanII GRGCYC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 3 cut(s) 340, 983, 1039
BccI CCATC 6 cut(s) 154, 697, 776, 959, 1145, 1186
BceAI ACGGC 1 cut(s) 739
BciT130I CCWGG 1 cut(s) 609
BciVI GTATCC 1 cut(s) 205
BcoDI GTCTC 2 cut(s) 30, 498
BfaI CTAG 4 cut(s) 147, 410, 1208, 1308
BfmI CTRYAG 2 cut(s) 255, 1214
BfuAI ACCTGC 1 cut(s) 1095
BfuI GTATCC 1 cut(s) 205
BisI GCNGC 4 cut(s) 329, 484, 972, 1053
BlsI GCNGC 4 cut(s) 330, 485, 973, 1054
Bme1390I CCNGG 1 cut(s) 609
Bme18I GGWCC 1 cut(s) 181
BmgT120I GGNCC 2 cut(s) 181, 1079
BmiI GGNNCC 5 cut(s) 311, 462, 933, 1021, 1080
BmrFI CCNGG 1 cut(s) 609
BmsI GCATC 1 cut(s) 45
BpuEI CTTGAG 1 cut(s) 1223
BsaBI GATNNNNATC 2 cut(s) 96, 1143
BsaI GGTCTC 2 cut(s) 30, 498
BsaJI CCNNGG 4 cut(s) 85, 299, 465, 608
BsaXI ACNNNNNCTCC 2 cut(s) 861, 891
Bsc4I CCNNNNNNNGG 1 cut(s) 1029
Bse3DI GCAATG 2 cut(s) 809, 835
Bse8I GATNNNNATC 2 cut(s) 96, 1143
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 4 cut(s) 85, 299, 465, 608
BseGI GGATG 2 cut(s) 350, 708
BseJI GATNNNNATC 2 cut(s) 96, 1143
BseLI CCNNNNNNNGG 1 cut(s) 1029
BseMI GCAATG 2 cut(s) 809, 835
BseMII CTCAG 2 cut(s) 237, 1269
BseRI GAGGAG 3 cut(s) 532, 546, 1183
BseSI GKGCMC 1 cut(s) 465
BseXI GCAGC 3 cut(s) 340, 983, 1039
Bsh1236I CGCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 287, 483, 1081
BshNI GGYRCC 3 cut(s) 460, 931, 1019
BsiHKAI GWGCWC 1 cut(s) 345
BsiWI CGTACG 1 cut(s) 756
BslFI GGGAC 2 cut(s) 625, 910
BslI CCNNNNNNNGG 1 cut(s) 1029
BsmAI GTCTC 2 cut(s) 30, 498
BsmFI GGGAC 2 cut(s) 625, 910
BsnI GGCC 3 cut(s) 287, 483, 1081
Bso31I GGTCTC 2 cut(s) 30, 498
Bsp1286I GDGCHC 2 cut(s) 345, 465
Bsp143I GATC 5 cut(s) 133, 309, 538, 589, 670
Bsp19I CCATGG 2 cut(s) 85, 465
BspACI CCGC 3 cut(s) 130, 484, 649
BspANI GGCC 3 cut(s) 287, 483, 1081
BspCNI CTCAG 2 cut(s) 238, 1268
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 5 cut(s) 311, 462, 933, 1021, 1080
BspMAI CTGCAG 1 cut(s) 1218
BspMI ACCTGC 1 cut(s) 1095
BspPI GGATC 4 cut(s) 304, 317, 584, 665
BspQI GCTCTTC 1 cut(s) 1247
BspT107I GGYRCC 3 cut(s) 460, 931, 1019
BspTNI GGTCTC 2 cut(s) 30, 498
BsrDI GCAATG 2 cut(s) 809, 835
BssECI CCNNGG 4 cut(s) 85, 299, 465, 608
BssMI GATC 5 cut(s) 133, 309, 538, 589, 670
BssT1I CCWWGG 3 cut(s) 85, 299, 465
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 3 cut(s) 548, 830, 1269
Bst6I CTCTTC 1 cut(s) 1247
BstDEI CTNAG 5 cut(s) 246, 317, 424, 1117, 1255
BstDSI CCRYGG 2 cut(s) 85, 465
BstENI CCTNNNNNAGG 1 cut(s) 1027
BstF5I GGATG 2 cut(s) 350, 708
BstFNI CGCG 1 cut(s) 132
BstKTI GATC 5 cut(s) 136, 312, 541, 592, 673
BstMAI GTCTC 2 cut(s) 30, 498
BstMBI GATC 5 cut(s) 133, 309, 538, 589, 670
BstMWI GCNNNNNNNGC 3 cut(s) 251, 1087, 1213
BstNI CCWGG 1 cut(s) 609
BstSCI CCNGG 1 cut(s) 607
BstSFI CTRYAG 2 cut(s) 255, 1214
BstSLI GKGCMC 1 cut(s) 465
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 3 cut(s) 340, 983, 1039
BstX2I RGATCY 1 cut(s) 309
BstXI CCANNNNNNTGG 2 cut(s) 149, 681
BstYI RGATCY 1 cut(s) 309
BsuI GTATCC 1 cut(s) 205
BsuRI GGCC 3 cut(s) 287, 483, 1081
BtgI CCRYGG 2 cut(s) 85, 465
BtsCI GGATG 2 cut(s) 350, 708
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 3 cut(s) 631, 666, 1008
BveI ACCTGC 1 cut(s) 1095
CaiI CAGNNNCTG 1 cut(s) 905
Cfr13I GGNCC 2 cut(s) 181, 1079
Csp6I GTAC 3 cut(s) 757, 1020, 1156
CviAII CATG 7 cut(s) 86, 332, 466, 740, 853, 893, 1273
CviQI GTAC 3 cut(s) 757, 1020, 1156
DdeI CTNAG 5 cut(s) 246, 317, 424, 1117, 1255
DpnI GATC 5 cut(s) 135, 311, 540, 591, 672
DpnII GATC 5 cut(s) 133, 309, 538, 589, 670
DraI TTTAAA 2 cut(s) 994, 1072
EaeI YGGCCR 1 cut(s) 481
Eam1104I CTCTTC 1 cut(s) 1247
EarI CTCTTC 1 cut(s) 1247
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 3 cut(s) 85, 299, 465
Eco24I GRGCYC 1 cut(s) 345
Eco31I GGTCTC 2 cut(s) 30, 498
Eco32I GATATC 1 cut(s) 884
Eco47I GGWCC 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 1 cut(s) 654
EcoICRI GAGCTC 1 cut(s) 343
EcoNI CCTNNNNNAGG 1 cut(s) 1027
EcoRII CCWGG 1 cut(s) 607
EcoRV GATATC 1 cut(s) 884
EcoT14I CCWWGG 3 cut(s) 85, 299, 465
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 3 cut(s) 85, 299, 465
FaeI CATG 7 cut(s) 89, 335, 469, 743, 856, 896, 1276
FalI AAGNNNNNCTT 2 cut(s) 1100, 1132
FaqI GGGAC 2 cut(s) 625, 910
FatI CATG 7 cut(s) 85, 331, 465, 739, 852, 892, 1272
FauNDI CATATG 1 cut(s) 680
Fnu4HI GCNGC 4 cut(s) 329, 484, 972, 1053
FokI GGATG 2 cut(s) 337, 715
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 4 cut(s) 329, 484, 972, 1053
FspBI CTAG 4 cut(s) 147, 410, 1208, 1308
GluI GCNGC 4 cut(s) 329, 484, 972, 1053
HaeIII GGCC 3 cut(s) 287, 483, 1081
Hin1II CATG 7 cut(s) 89, 335, 469, 743, 856, 896, 1276
HindIII AAGCTT 2 cut(s) 174, 417
HinfI GANTC 1 cut(s) 369
HphI GGTGA 3 cut(s) 109, 937, 1106
Hpy188I TCNGA 4 cut(s) 273, 309, 355, 1337
Hpy188III TCNNGA 4 cut(s) 1148, 1165, 1284, 1308
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 6 cut(s) 194, 270, 323, 330, 574, 1033
HpyCH4III ACNGT 3 cut(s) 548, 830, 1269
HpyCH4IV ACGT 1 cut(s) 847
HpyCH4V TGCA 8 cut(s) 36, 64, 659, 835, 852, 1055, 1216, 1331
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 1087, 1213
HpyF3I CTNAG 5 cut(s) 246, 317, 424, 1117, 1255
HpySE526I ACGT 1 cut(s) 847
Hsp92II CATG 7 cut(s) 89, 335, 469, 743, 856, 896, 1276
KpnI GGTACC 1 cut(s) 1023
Kzo9I GATC 5 cut(s) 133, 309, 538, 589, 670
LguI GCTCTTC 1 cut(s) 1247
LmnI GCTCC 4 cut(s) 340, 555, 622, 968
Lsp1109I GCAGC 3 cut(s) 340, 983, 1039
LweI GCATC 1 cut(s) 45
MaeI CTAG 4 cut(s) 147, 410, 1208, 1308
MaeII ACGT 1 cut(s) 847
MaeIII GTNAC 3 cut(s) 236, 259, 1263
MalI GATC 5 cut(s) 135, 311, 540, 591, 672
MboI GATC 5 cut(s) 133, 309, 538, 589, 670
MboII GAAGA 5 cut(s) 64, 176, 830, 1201, 1234
MfeI CAATTG 1 cut(s) 1314
MflI RGATCY 1 cut(s) 309
MhlI GDGCHC 2 cut(s) 345, 465
MluCI AATT 9 cut(s) 168, 216, 550, 654, 1065, 1073, 1099, 1287, 1314
MmeI TCCRAC 2 cut(s) 251, 859
MroXI GAANNNNTTC 2 cut(s) 373, 396
MseI TTAA 6 cut(s) 171, 219, 543, 687, 993, 1071
MslI CAYNNNNRTG 2 cut(s) 147, 679
MspA1I CMGCKG 1 cut(s) 1052
MspR9I CCNGG 1 cut(s) 609
MunI CAATTG 1 cut(s) 1314
MvaI CCWGG 1 cut(s) 609
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 3 cut(s) 251, 1087, 1213
NcoI CCATGG 2 cut(s) 85, 465
NdeI CATATG 1 cut(s) 680
NdeII GATC 5 cut(s) 133, 309, 538, 589, 670
NlaIII CATG 7 cut(s) 89, 335, 469, 743, 856, 896, 1276
NlaIV GGNNCC 5 cut(s) 311, 462, 933, 1021, 1080
PciSI GCTCTTC 1 cut(s) 1247
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PdmI GAANNNNTTC 2 cut(s) 373, 396
PfeI GAWTC 1 cut(s) 369
Pfl23II CGTACG 1 cut(s) 756
PkrI GCNGC 4 cut(s) 330, 485, 973, 1054
Psp124BI GAGCTC 1 cut(s) 345
Psp1406I AACGTT 1 cut(s) 847
Psp6I CCWGG 1 cut(s) 607
PspGI CCWGG 1 cut(s) 607
PspLI CGTACG 1 cut(s) 756
PspN4I GGNNCC 5 cut(s) 311, 462, 933, 1021, 1080
PspPI GGNCC 2 cut(s) 181, 1079
PstI CTGCAG 1 cut(s) 1218
PstNI CAGNNNCTG 1 cut(s) 905
PsuI RGATCY 1 cut(s) 309
PvuII CAGCTG 1 cut(s) 1052
RsaI GTAC 3 cut(s) 758, 1021, 1157
RsaNI GTAC 3 cut(s) 757, 1020, 1156
RseI CAYNNNNRTG 2 cut(s) 147, 679
SacI GAGCTC 1 cut(s) 345
SapI GCTCTTC 1 cut(s) 1247
SaqAI TTAA 6 cut(s) 171, 219, 543, 687, 993, 1071
SatI GCNGC 4 cut(s) 329, 484, 972, 1053
Sau3AI GATC 5 cut(s) 133, 309, 538, 589, 670
Sau96I GGNCC 2 cut(s) 181, 1079
ScrFI CCNGG 1 cut(s) 609
SduI GDGCHC 2 cut(s) 345, 465
SfaNI GCATC 1 cut(s) 45
SfcI CTRYAG 2 cut(s) 255, 1214
SinI GGWCC 1 cut(s) 181
SmiMI CAYNNNNRTG 2 cut(s) 147, 679
SmlI CTYRAG 1 cut(s) 1202
SmoI CTYRAG 1 cut(s) 1202
Sse9I AATT 9 cut(s) 168, 216, 550, 654, 1065, 1073, 1099, 1287, 1314
SsiI CCGC 3 cut(s) 130, 484, 649
SspMI CTAG 4 cut(s) 147, 410, 1208, 1308
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 607
StyI CCWWGG 3 cut(s) 85, 299, 465
TaaI ACNGT 3 cut(s) 548, 830, 1269
TaiI ACGT 1 cut(s) 850
TaqI TCGA 2 cut(s) 187, 1164
TasI AATT 9 cut(s) 168, 216, 550, 654, 1065, 1073, 1099, 1287, 1314
TauI GCSGC 1 cut(s) 486
TfiI GAWTC 1 cut(s) 369
Tru1I TTAA 6 cut(s) 171, 219, 543, 687, 993, 1071
Tru9I TTAA 6 cut(s) 171, 219, 543, 687, 993, 1071
TscAI CASTG 3 cut(s) 631, 666, 1015
TseI GCWGC 3 cut(s) 328, 971, 1052
TspDTI ATGAA 3 cut(s) 818, 1056, 1261
TspGWI ACGGA 2 cut(s) 67, 582
TspRI CASTG 3 cut(s) 631, 666, 1015
VpaK11BI GGWCC 1 cut(s) 181
XagI CCTNNNNNAGG 1 cut(s) 1027
XapI RAATTY 1 cut(s) 1287
XbaI TCTAGA 1 cut(s) 1307
XmnI GAANNNNTTC 2 cut(s) 373, 396
XspI CTAG 4 cut(s) 147, 410, 1208, 1308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.