MD12G1003300.v1.1

Zn_pept

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008016
Physical Location & Seq
Reverse (-)
498017 .. 501368
3352 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1003300.v1.1.491

Sequence Viewer

Length: 1515 bp
ATGAAAACCCAAACCATGGCTCATCTCCTTGTTCCTCCGATGATTGCCAATGCCATGGCTCATCTCCTTCCTCTCTCCTCTCCTTTATCCATCACTCTCTGCTTCTTTGCGTGGTTGCAAGCTTTCTTGGTCGTCCATGGCAATACTAATAACATCACCAATCCTTCTTCTCTCACCCCCATGAACCGCGATCTCTACCATTCCAGTGGGGATTTGATGGAAGAAATTAAAGCTCTCGTCTTTCGGCACCCAGACGAGCTCACTTTGGATACAATGAAATCTCGAAACAAGGGCTACTCTGCTGAGATCGCAATAGTTACCTATTGCCGGAGAAGGCAAGAGACTGATGACCAGCCAAAGTTACGGATCCTTCTTAGTTTTGGGCAGCATGGAAGGGAGCTTATTACATCCGAGCTTGCATTACGAATCCTATCAATCTTGAGTAAAGAACAGTTTCTACCCAACCTGGACCCCGTGTCCTTAGACAGTACCCTTGACAAGCTCTTGATAAAGGTGGTTCCGATGGAAAACTTAAATGGCCGTAGACTTGTTGAAGCAGGAGACCTCTGTGAGAGGAGAAATGGGAGGGGAGTTGATCCCAACCGTAATTGGAGCGTGGATTGGGGAAAAAAAAGAGAAGACTATTCTTTTGCTTTTATTTTGTTTTTCTATCCCAGGATTATGATCCATCCTGGAACTGCTCCTTTCAGCGAGCCTGAAACTCAGATAATGCGGAAAGTTTCCATGTCATTTGATCCACACATATGGATTAATATACACTCTGGGATGGAGGCTCTATTTATGCCGTATGACCATAAGAACACAACCCCAGATGGACTTTCCTCCCACCAGATGAAGTCATTGCTTGAAGAACTCAACCAACTGCATTTCCAAAAGCGTTGCATGGTTGGGTCTGGTGGAGGTTCTGTTGGGTACCTGGCACATGGGACAGCAACTGATTTTATGTTCGACATTGTAAAGGTGCCAATGGCTTTCACCTTTGAGGTTTGTCAAGTTATCCCTCATTTTCCCCTCAAAGTTCTGATCTTAAAGCAACCTGACCCAGACAAATTTGCATCCTTTTTCGTTGTGACTCAGATATATGGAGATGGAGCAGCCTCATCAAGAGATTGCTTCAAAATGTTCAATCCCACAGAATTGGGTACCTTCAATTATAAATCTTTTCGCTTTCAGAGAGTTCTCAATGAATGGTCAGCTGCATTTTTCACAATTTTTAAATTGGGGCCAGAGCAGCTTGGTGAAAATTATTCAAAGTCTTCTGTGCCCACCTTATTAGACAAGTGGGTATCCATAGATGAGTATCTTGAAGGGTACTTAGTCGAGAGGAGTAGTAGATACGGAAAGAAGATGGAGGTGCTTGAACTAGGAATGCAGGAGATAAGAACATATTTCAGGCTCTTCTTGTTATCGTCTGTTCTGTTAATGTTCATGTTCTGTTCCAGAATTTCAAAAAGCAAGTGTGCCAGACCAATTGTTTCTGCTATTGCAATCTGA

Protein Analysis

505

Amino Acids

57.53

Weight (kDa)

8.3

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M14 PF00246 75 - 337 3.2e-20 Zinc carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1176
Acc65I GGTACC 2 cut(s) 933, 1163
AccB1I GGYRCC 4 cut(s) 246, 933, 982, 1163
AccB7I CCANNNNNTGG 1 cut(s) 16
AccI GTMKAC 1 cut(s) 544
AccII CGCG 1 cut(s) 189
AciI CCGC 2 cut(s) 187, 733
AclWI GGATC 5 cut(s) 361, 374, 590, 679, 749
AcoI YGGCCR 1 cut(s) 538
AcsI RAATTY 2 cut(s) 1070, 1464
AfaI GTAC 4 cut(s) 490, 935, 1165, 1334
AfiI CCNNNNNNNGG 2 cut(s) 16, 327
AgsI TTSAA 9 cut(s) 554, 869, 1138, 1147, 1171, 1272, 1328, 1382, 1470
AhdI GACNNNNNGTC 1 cut(s) 475
AjnI CCWGG 4 cut(s) 465, 674, 691, 936
AluBI AGCT 8 cut(s) 122, 233, 259, 400, 415, 502, 1217, 1255
AluI AGCT 8 cut(s) 122, 233, 259, 400, 415, 502, 1217, 1255
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 2 cut(s) 335, 555
AlwI GGATC 5 cut(s) 361, 374, 590, 679, 749
AlwNI CAGNNNCTG 1 cut(s) 956
AoxI GGCC 2 cut(s) 538, 1244
ApeKI GCWGC 4 cut(s) 385, 1115, 1217, 1252
ApoI RAATTY 2 cut(s) 1070, 1464
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AseI ATTAAT 1 cut(s) 771
Asp700I GAANNNNTTC 1 cut(s) 453
Asp718I GGTACC 2 cut(s) 933, 1163
AspS9I GGNCC 2 cut(s) 469, 1244
AsuHPI GGTGA 4 cut(s) 148, 166, 988, 1271
AvaII GGWCC 1 cut(s) 469
BaeGI GKGCMC 1 cut(s) 1287
BamHI GGATCC 1 cut(s) 366
BanI GGYRCC 4 cut(s) 246, 933, 982, 1163
BanII GRGCYC 1 cut(s) 261
BbsI GAAGAC 2 cut(s) 645, 1269
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 4 cut(s) 397, 1127, 1204, 1264
BccI CCATC 8 cut(s) 98, 211, 517, 696, 781, 827, 1103, 1363
BceAI ACGGC 2 cut(s) 525, 790
BciT130I CCWGG 4 cut(s) 467, 676, 693, 938
BciVI GTATCC 2 cut(s) 262, 1318
BcoDI GTCTC 2 cut(s) 335, 555
BfaI CTAG 1 cut(s) 1385
BfuI GTATCC 2 cut(s) 262, 1318
BisI GCNGC 4 cut(s) 386, 1116, 1218, 1253
BlsI GCNGC 4 cut(s) 387, 1117, 1219, 1254
Bme1390I CCNGG 4 cut(s) 467, 676, 693, 938
Bme18I GGWCC 1 cut(s) 469
BmeRI GACNNNNNGTC 1 cut(s) 475
BmgT120I GGNCC 2 cut(s) 469, 1244
BmiI GGNNCC 8 cut(s) 248, 368, 471, 519, 935, 984, 1165, 1245
BmrFI CCNGG 4 cut(s) 467, 676, 693, 938
BmsI GCATC 1 cut(s) 1085
BpiI GAAGAC 2 cut(s) 645, 1269
BpuEI CTTGAG 1 cut(s) 460
BsaBI GATNNNNATC 2 cut(s) 683, 1320
BsaI GGTCTC 1 cut(s) 555
BsaJI CCNNGG 4 cut(s) 15, 54, 136, 674
BsaXI ACNNNNNCTCC 2 cut(s) 912, 942
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 327
Bse1I ACTGG 1 cut(s) 204
Bse3DI GCAATG 1 cut(s) 860
Bse8I GATNNNNATC 2 cut(s) 683, 1320
BseBI CCWGG 4 cut(s) 467, 676, 693, 938
BseDI CCNNGG 4 cut(s) 15, 54, 136, 674
BseGI GGATG 4 cut(s) 407, 688, 792, 1076
BseJI GATNNNNATC 2 cut(s) 683, 1320
BseLI CCNNNNNNNGG 2 cut(s) 16, 327
BseMI GCAATG 1 cut(s) 860
BseMII CTCAG 3 cut(s) 294, 737, 1109
BseNI ACTGG 1 cut(s) 204
BseRI GAGGAG 3 cut(s) 67, 589, 1360
BseSI GKGCMC 1 cut(s) 1287
BseXI GCAGC 4 cut(s) 397, 1127, 1204, 1264
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 2 cut(s) 540, 1246
BshNI GGYRCC 4 cut(s) 246, 933, 982, 1163
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 1 cut(s) 328
BslFI GGGAC 1 cut(s) 961
BslI CCNNNNNNNGG 2 cut(s) 16, 327
BsmAI GTCTC 2 cut(s) 335, 555
BsmFI GGGAC 1 cut(s) 961
BsmI GAATGC 1 cut(s) 1395
BsnI GGCC 2 cut(s) 540, 1246
Bso31I GGTCTC 1 cut(s) 555
Bsp1286I GDGCHC 2 cut(s) 261, 1287
Bsp143I GATC 7 cut(s) 190, 306, 366, 595, 684, 754, 1044
Bsp19I CCATGG 3 cut(s) 15, 54, 136
BspACI CCGC 2 cut(s) 187, 733
BspANI GGCC 2 cut(s) 540, 1246
BspCNI CTCAG 3 cut(s) 295, 736, 1108
BspFNI CGCG 1 cut(s) 189
BspLI GGNNCC 8 cut(s) 248, 368, 471, 519, 935, 984, 1165, 1245
BspPI GGATC 5 cut(s) 361, 374, 590, 679, 749
BspQI GCTCTTC 1 cut(s) 1424
BspT107I GGYRCC 4 cut(s) 246, 933, 982, 1163
BspTNI GGTCTC 1 cut(s) 555
BsrDI GCAATG 1 cut(s) 860
BsrI ACTGG 1 cut(s) 204
BssECI CCNNGG 4 cut(s) 15, 54, 136, 674
BssMI GATC 7 cut(s) 190, 306, 366, 595, 684, 754, 1044
BssT1I CCWWGG 3 cut(s) 15, 54, 136
Bst2UI CCWGG 4 cut(s) 467, 676, 693, 938
Bst4CI ACNGT 3 cut(s) 453, 488, 605
Bst6I CTCTTC 1 cut(s) 1424
BstC8I GCNNGC 3 cut(s) 120, 417, 713
BstDEI CTNAG 6 cut(s) 303, 374, 481, 723, 1095, 1336
BstDSI CCRYGG 3 cut(s) 15, 54, 136
BstF5I GGATG 4 cut(s) 407, 688, 792, 1076
BstFNI CGCG 1 cut(s) 189
BstKTI GATC 7 cut(s) 193, 309, 369, 598, 687, 757, 1047
BstMAI GTCTC 2 cut(s) 335, 555
BstMBI GATC 7 cut(s) 190, 306, 366, 595, 684, 754, 1044
BstMWI GCNNNNNNNGC 2 cut(s) 308, 1252
BstNI CCWGG 4 cut(s) 467, 676, 693, 938
BstSCI CCNGG 4 cut(s) 465, 674, 691, 936
BstSLI GKGCMC 1 cut(s) 1287
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 4 cut(s) 397, 1127, 1204, 1264
BstV2I GAAGAC 2 cut(s) 645, 1269
BstX2I RGATCY 1 cut(s) 366
BstXI CCANNNNNNTGG 4 cut(s) 55, 206, 765, 1159
BstYI RGATCY 1 cut(s) 366
BsuI GTATCC 2 cut(s) 262, 1318
BsuRI GGCC 2 cut(s) 540, 1246
BtgI CCRYGG 3 cut(s) 15, 54, 136
BtsCI GGATG 4 cut(s) 407, 688, 792, 1076
BtsIMutI CAGTG 1 cut(s) 211
Cac8I GCNNGC 3 cut(s) 120, 417, 713
CaiI CAGNNNCTG 1 cut(s) 956
Cfr13I GGNCC 2 cut(s) 469, 1244
Csp6I GTAC 4 cut(s) 489, 934, 1164, 1333
CviAII CATG 9 cut(s) 16, 55, 137, 181, 389, 745, 904, 944, 1450
CviQI GTAC 4 cut(s) 489, 934, 1164, 1333
DdeI CTNAG 6 cut(s) 303, 374, 481, 723, 1095, 1336
DpnI GATC 7 cut(s) 192, 308, 368, 597, 686, 756, 1046
DpnII GATC 7 cut(s) 190, 306, 366, 595, 684, 754, 1044
DraI TTTAAA 1 cut(s) 1237
DriI GACNNNNNGTC 1 cut(s) 475
EaeI YGGCCR 1 cut(s) 538
Eam1104I CTCTTC 1 cut(s) 1424
Eam1105I GACNNNNNGTC 1 cut(s) 475
EarI CTCTTC 1 cut(s) 1424
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 3 cut(s) 15, 54, 136
Eco24I GRGCYC 1 cut(s) 261
Eco31I GGTCTC 1 cut(s) 555
Eco47I GGWCC 1 cut(s) 469
Eco53kI GAGCTC 1 cut(s) 259
EcoICRI GAGCTC 1 cut(s) 259
EcoRII CCWGG 4 cut(s) 465, 674, 691, 936
EcoT14I CCWWGG 3 cut(s) 15, 54, 136
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 3 cut(s) 15, 54, 136
FaeI CATG 9 cut(s) 19, 58, 140, 184, 392, 748, 907, 947, 1453
FaqI GGGAC 1 cut(s) 961
FatI CATG 9 cut(s) 15, 54, 136, 180, 388, 744, 903, 943, 1449
FauNDI CATATG 1 cut(s) 764
FblI GTMKAC 1 cut(s) 544
Fnu4HI GCNGC 4 cut(s) 386, 1116, 1218, 1253
FokI GGATG 4 cut(s) 394, 675, 799, 1063
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 4 cut(s) 386, 1116, 1218, 1253
FspBI CTAG 1 cut(s) 1385
GluI GCNGC 4 cut(s) 386, 1116, 1218, 1253
HaeIII GGCC 2 cut(s) 540, 1246
HapII CCGG 1 cut(s) 328
Hin1II CATG 9 cut(s) 19, 58, 140, 184, 392, 748, 907, 947, 1453
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 2 cut(s) 426, 1093
HpaII CCGG 1 cut(s) 328
HphI GGTGA 4 cut(s) 148, 166, 988, 1271
Hpy166II GTNNAC 1 cut(s) 545
Hpy188I TCNGA 8 cut(s) 39, 412, 522, 726, 1044, 1098, 1194, 1514
Hpy188III TCNNGA 7 cut(s) 282, 439, 505, 1125, 1325, 1342, 1461
Hpy8I GTNNAC 1 cut(s) 545
HpyAV CCTTC 7 cut(s) 77, 174, 327, 380, 387, 1177, 1322
HpyCH4III ACNGT 3 cut(s) 453, 488, 605
HpyCH4V TGCA 8 cut(s) 118, 419, 886, 903, 1076, 1220, 1393, 1508
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 1252
HpyF3I CTNAG 6 cut(s) 303, 374, 481, 723, 1095, 1336
Hsp92II CATG 9 cut(s) 19, 58, 140, 184, 392, 748, 907, 947, 1453
KpnI GGTACC 2 cut(s) 937, 1167
Kzo9I GATC 7 cut(s) 190, 306, 366, 595, 684, 754, 1044
LguI GCTCTTC 1 cut(s) 1424
LmnI GCTCC 4 cut(s) 397, 612, 706, 1112
Lsp1109I GCAGC 4 cut(s) 397, 1127, 1204, 1264
LweI GCATC 1 cut(s) 1085
MaeI CTAG 1 cut(s) 1385
MaeIII GTNAC 3 cut(s) 316, 360, 1090
MalI GATC 7 cut(s) 192, 308, 368, 597, 686, 756, 1046
MboI GATC 7 cut(s) 190, 306, 366, 595, 684, 754, 1044
MboII GAAGA 7 cut(s) 159, 233, 650, 881, 1269, 1378, 1411
MfeI CAATTG 1 cut(s) 1491
MflI RGATCY 1 cut(s) 366
MhlI GDGCHC 2 cut(s) 261, 1287
MlyI GAGTC 1 cut(s) 1087
MroXI GAANNNNTTC 1 cut(s) 453
MseI TTAA 6 cut(s) 228, 533, 771, 1049, 1236, 1442
MslI CAYNNNNRTG 3 cut(s) 179, 204, 763
MspA1I CMGCKG 1 cut(s) 1217
MspI CCGG 1 cut(s) 328
MspR9I CCNGG 4 cut(s) 467, 676, 693, 938
MunI CAATTG 1 cut(s) 1491
Mva1269I GAATGC 1 cut(s) 1395
MvaI CCWGG 4 cut(s) 467, 676, 693, 938
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 2 cut(s) 308, 1252
NcoI CCATGG 3 cut(s) 15, 54, 136
NdeI CATATG 1 cut(s) 764
NdeII GATC 7 cut(s) 190, 306, 366, 595, 684, 754, 1044
NlaIII CATG 9 cut(s) 19, 58, 140, 184, 392, 748, 907, 947, 1453
NlaIV GGNNCC 8 cut(s) 248, 368, 471, 519, 935, 984, 1165, 1245
NmuCI GTSAC 1 cut(s) 1090
PciSI GCTCTTC 1 cut(s) 1424
PctI GAATGC 1 cut(s) 1395
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 1 cut(s) 426
PflMI CCANNNNNTGG 1 cut(s) 16
PfoI TCCNGGA 1 cut(s) 691
PkrI GCNGC 4 cut(s) 387, 1117, 1219, 1254
PleI GAGTC 1 cut(s) 1087
PpsI GAGTC 1 cut(s) 1087
PshBI ATTAAT 1 cut(s) 771
PsiI TTATAA 1 cut(s) 1176
Psp124BI GAGCTC 1 cut(s) 261
Psp6I CCWGG 4 cut(s) 465, 674, 691, 936
PspGI CCWGG 4 cut(s) 465, 674, 691, 936
PspN4I GGNNCC 8 cut(s) 248, 368, 471, 519, 935, 984, 1165, 1245
PspPI GGNCC 2 cut(s) 469, 1244
PsrI GAACNNNNNNTAC 2 cut(s) 441, 473
PstNI CAGNNNCTG 1 cut(s) 956
PsuI RGATCY 1 cut(s) 366
PvuII CAGCTG 1 cut(s) 1217
RsaI GTAC 4 cut(s) 490, 935, 1165, 1334
RsaNI GTAC 4 cut(s) 489, 934, 1164, 1333
RseI CAYNNNNRTG 3 cut(s) 179, 204, 763
SacI GAGCTC 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 1424
SaqAI TTAA 6 cut(s) 228, 533, 771, 1049, 1236, 1442
SatI GCNGC 4 cut(s) 386, 1116, 1218, 1253
Sau3AI GATC 7 cut(s) 190, 306, 366, 595, 684, 754, 1044
Sau96I GGNCC 2 cut(s) 469, 1244
SchI GAGTC 1 cut(s) 1087
ScrFI CCNGG 4 cut(s) 467, 676, 693, 938
SduI GDGCHC 2 cut(s) 261, 1287
SfaNI GCATC 1 cut(s) 1085
SinI GGWCC 1 cut(s) 469
SmiMI CAYNNNNRTG 3 cut(s) 179, 204, 763
SmlI CTYRAG 1 cut(s) 439
SmoI CTYRAG 1 cut(s) 439
SsiI CCGC 2 cut(s) 187, 733
SspMI CTAG 1 cut(s) 1385
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 4 cut(s) 465, 674, 691, 936
StyI CCWWGG 3 cut(s) 15, 54, 136
TaaI ACNGT 3 cut(s) 453, 488, 605
TaqI TCGA 3 cut(s) 283, 969, 1341
TfiI GAWTC 1 cut(s) 426
Tru1I TTAA 6 cut(s) 228, 533, 771, 1049, 1236, 1442
Tru9I TTAA 6 cut(s) 228, 533, 771, 1049, 1236, 1442
TscAI CASTG 1 cut(s) 211
TseFI GTSAC 1 cut(s) 1090
TseI GCWGC 4 cut(s) 385, 1115, 1217, 1252
Tsp45I GTSAC 1 cut(s) 1090
TspDTI ATGAA 6 cut(s) 17, 197, 290, 869, 1221, 1438
TspGWI ACGGA 2 cut(s) 379, 1374
TspRI CASTG 1 cut(s) 211
Van91I CCANNNNNTGG 1 cut(s) 16
VpaK11BI GGWCC 1 cut(s) 469
VspI ATTAAT 1 cut(s) 771
XapI RAATTY 2 cut(s) 1070, 1464
XmiI GTMKAC 1 cut(s) 544
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 1 cut(s) 1385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.