Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008016

Physical Location & Seq

Genomic Coordinates Forward (+)

117044 .. 122585

Gene Structure 5542 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1002000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1317 bp

ATGGCTCATCTCCTTCCTCTCTCCTCTCCCTTATCCATCACTCTCTGCTTCTTTGCGTGGTTACAAGCTTTCTTGGTCGTCCATGGCAATAGTAATCTCACCCACCCTTCTACTCTCACCCCCATCAACAGCGATCTCTATCACTCCAGTGAGGATTTGATGGAAGAAATTAAAGCTCTCGTCTTTCGGCACCCAGACGAGCTCACTTTGGATACAATTAAATCTCAAAACAAGGGCTACTCTGCTGAGATCGCAATAGTTACCTATAGTCAGAGAAGGCAAGAGACTGATGACCAGCCAAAATTTCGAATCCTTCTTAGTTTTGGGCAGCATGGAAGGGAGCTTATTACATCCGAGCTTGCATTGCGAATCCTATCAATCTTGAGTAAAGAACAGTTTCTACCCAACCTTGACCCAGCCTCCTTAGACAGTACCCTTGACAAGCTCTTGATAAAGGTGGTTCCAATGGAAAACTTAAATGGCCGTAAACTTGTTGAAGCAGGAGACCTCTGTGAGAGGAGAAATGGGAGGGGAGTTGATCTCAACCGTAATTGGAGCGTAGATTGGGGCAAAAAAGAGAAGGATTATGATCCATATGAGGAAAATCCTGGAACTGCTCCTTTCAGCGAGCCTGAAACTCAGATAATACGGAAAGTTGCCGTGTCATTTGATCCACACATCTGGATTAATATACACTCTGGGATGGAGGCTCTATTTATGCCGTACGACCATAAGAACACAACCCCAGATGGAGTTTCCTCCCACCAGATGAAGTCATTGCTTGAAGAACTCAACCAACTGCACTGCCAAAAGCGTTGCATGGTTGGGTCTGGTGGAGGTTCTGTTGGGTACCTGGCACATGGGACAGCAACTGATTTTATGTTCGACATTGTAAGGGTGCCAATGGCTTTCACCTTTGAGATATATGGAGATGGAGCAGCCTCATCAAGAGATTGCTTCAAAATGTTCAATCCCACTGACTTCGTTACCTTCAATAGAGTTCTCAATGAATGGTCTGCTGCATTTTTCACAATTTTTAAATTGGGGCCAGACCAGCTTGGTGAAAATTATTCAAAGGCTTCTGTGCCCACCTTATTAGACAAGTGGGTATCCATAGATGAGTATCTTGAAGGTTATTTAGTCGAGAGGAGTAGTAGATATGGAAAGAAGATGGAGGTGCTTGAACTAGGAATGCAGGAGATAAGAACATATTTCAGGCTCTTCTTGTTATCGTCTGTTCTGTTAATGTTCATGTTCTGTTCCAGAATTTCAAAAAGCAAGTGTGCTAGACCAATTGTTTCTGCTATTGCAATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004180 GO:0004181 GO:0005575 GO:0005576 GO:0005615 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0016787 GO:0019538 GO:0043170 GO:0044238 GO:0044421 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M14

Protein Analysis

439

Amino Acids

49.65

Weight (kDa)

5.79

Isoelectric Point (pI)

34.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M14	PF00246	55 - 321	2.4e-27	Zinc carboxypeptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008016)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42320 AT5G42320
fragaria_vesca	FvH4_2g37450
malus_domestica	MD00G1076900.v1.1 MD08G1002400.v1.1 MD12G1003300.v1.1 MD15G1002000.v1.1
prunus_persica	Prupe.1G354500_v2.0.a1 Prupe.1G354500_v2.0.a1 Prupe.1G354500_v2.0.a1
pyrus_communis	pycom08g00210 pycom15g00090
rosa_chinensis	RchiOBHm_Chr6g0304271
rosa_laevigata	RLG00000010996
rosa_multiflora	Rmu_ssc0000218.1_g000027
rosa_roxburghii	Rroxscaffold_7G00163890
rosa_rugosa	Rorug05G0331800 Rorug06G0329400 Rorug06G0329500
rosa_samantha	Rh6AG443000 Rh6BG459500 Rh6CG455700 Rh6DG442500
rosa_wichuraiana	Rw6G038430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	849
AccB1I	GGYRCC	3 cut(s)	189, 849, 898
AclWI	GGATC	2 cut(s)	584, 665
AcoI	YGGCCR	1 cut(s)	481
AcsI	RAATTY	2 cut(s)	302, 1266
AfaI	GTAC	3 cut(s)	433, 725, 851
AgsI	TTSAA	9 cut(s)	497, 785, 961, 970, 994, 1074, 1130, 1184, 1272
AjnI	CCWGG	2 cut(s)	607, 852
AleI	CACNNNNGTG	1 cut(s)	147
AluBI	AGCT	7 cut(s)	68, 176, 202, 343, 358, 445, 1057
AluI	AGCT	7 cut(s)	68, 176, 202, 343, 358, 445, 1057
Alw21I	GWGCWC	1 cut(s)	204
Alw26I	GTCTC	2 cut(s)	278, 498
AlwI	GGATC	2 cut(s)	584, 665
AlwNI	CAGNNNCTG	1 cut(s)	872
AoxI	GGCC	2 cut(s)	481, 1046
ApeKI	GCWGC	3 cut(s)	328, 938, 1019
ApoI	RAATTY	2 cut(s)	302, 1266
AseI	ATTAAT	1 cut(s)	687
Asp700I	GAANNNNTTC	1 cut(s)	396
Asp718I	GGTACC	1 cut(s)	849
AspS9I	GGNCC	1 cut(s)	1046
AsuHPI	GGTGA	4 cut(s)	91, 109, 904, 1073
AsuII	TTCGAA	1 cut(s)	307
BaeGI	GKGCMC	1 cut(s)	1089
BanI	GGYRCC	3 cut(s)	189, 849, 898
BanII	GRGCYC	1 cut(s)	204
Bbv12I	GWGCWC	1 cut(s)	204
BbvI	GCAGC	3 cut(s)	340, 950, 1006
BccI	CCATC	7 cut(s)	44, 131, 154, 697, 743, 926, 1165
BceAI	ACGGC	3 cut(s)	468, 644, 706
BciT130I	CCWGG	2 cut(s)	609, 854
BciVI	GTATCC	2 cut(s)	205, 1120
BcoDI	GTCTC	2 cut(s)	278, 498
BfaI	CTAG	2 cut(s)	1187, 1287
BfmI	CTRYAG	1 cut(s)	265
BfuI	GTATCC	2 cut(s)	205, 1120
BisI	GCNGC	3 cut(s)	329, 939, 1020
BlsI	GCNGC	3 cut(s)	330, 940, 1021
Bme1390I	CCNGG	2 cut(s)	609, 854
BmgT120I	GGNCC	1 cut(s)	1046
BmiI	GGNNCC	5 cut(s)	191, 462, 851, 900, 1047
BmrFI	CCNGG	2 cut(s)	609, 854
BplI	GAGNNNNNCTC	2 cut(s)	525, 557
BpmI	CTGGAG	1 cut(s)	130
Bpu14I	TTCGAA	1 cut(s)	307
BpuEI	CTTGAG	1 cut(s)	403
BsaBI	GATNNNNATC	3 cut(s)	138, 588, 1122
BsaI	GGTCTC	1 cut(s)	498
BsaJI	CCNNGG	1 cut(s)	82
BsaXI	ACNNNNNCTCC	4 cut(s)	404, 434, 828, 858
Bse1I	ACTGG	1 cut(s)	147
Bse3DI	GCAATG	2 cut(s)	362, 776
Bse8I	GATNNNNATC	3 cut(s)	138, 588, 1122
BseBI	CCWGG	2 cut(s)	609, 854
BseDI	CCNNGG	1 cut(s)	82
BseGI	GGATG	2 cut(s)	350, 708
BseJI	GATNNNNATC	3 cut(s)	138, 588, 1122
BseMI	GCAATG	2 cut(s)	362, 776
BseMII	CTCAG	2 cut(s)	237, 653
BseNI	ACTGG	1 cut(s)	147
BseRI	GAGGAG	3 cut(s)	13, 532, 1162
BseSI	GKGCMC	1 cut(s)	1089
BseXI	GCAGC	3 cut(s)	340, 950, 1006
BseYI	CCCAGC	1 cut(s)	415
BsgI	GTGCAG	1 cut(s)	785
BshFI	GGCC	2 cut(s)	483, 1048
BshNI	GGYRCC	3 cut(s)	189, 849, 898
BsiHKAI	GWGCWC	1 cut(s)	204
BsiWI	CGTACG	1 cut(s)	723
BslFI	GGGAC	1 cut(s)	877
BsmAI	GTCTC	2 cut(s)	278, 498
BsmFI	GGGAC	1 cut(s)	877
BsmI	GAATGC	1 cut(s)	1197
BsnI	GGCC	2 cut(s)	483, 1048
Bso31I	GGTCTC	1 cut(s)	498
Bsp119I	TTCGAA	1 cut(s)	307
Bsp1286I	GDGCHC	2 cut(s)	204, 1089
Bsp143I	GATC	5 cut(s)	133, 249, 538, 589, 670
Bsp19I	CCATGG	1 cut(s)	82
BspANI	GGCC	2 cut(s)	483, 1048
BspCNI	CTCAG	2 cut(s)	238, 652
BspLI	GGNNCC	5 cut(s)	191, 462, 851, 900, 1047
BspPI	GGATC	2 cut(s)	584, 665
BspQI	GCTCTTC	1 cut(s)	1226
BspT104I	TTCGAA	1 cut(s)	307
BspT107I	GGYRCC	3 cut(s)	189, 849, 898
BspTNI	GGTCTC	1 cut(s)	498
BsrDI	GCAATG	2 cut(s)	362, 776
BsrI	ACTGG	1 cut(s)	147
BssECI	CCNNGG	1 cut(s)	82
BssMI	GATC	5 cut(s)	133, 249, 538, 589, 670
BssT1I	CCWWGG	1 cut(s)	82
Bst2UI	CCWGG	2 cut(s)	609, 854
Bst4CI	ACNGT	3 cut(s)	396, 431, 548
Bst6I	CTCTTC	1 cut(s)	1226
BstBI	TTCGAA	1 cut(s)	307
BstC8I	GCNNGC	2 cut(s)	360, 629
BstDEI	CTNAG	4 cut(s)	246, 317, 424, 639
BstDSI	CCRYGG	1 cut(s)	82
BstF5I	GGATG	2 cut(s)	350, 708
BstKTI	GATC	5 cut(s)	136, 252, 541, 592, 673
BstMAI	GTCTC	2 cut(s)	278, 498
BstMBI	GATC	5 cut(s)	133, 249, 538, 589, 670
BstMWI	GCNNNNNNNGC	3 cut(s)	251, 364, 1054
BstNI	CCWGG	2 cut(s)	609, 854
BstSCI	CCNGG	2 cut(s)	607, 852
BstSFI	CTRYAG	1 cut(s)	265
BstSLI	GKGCMC	1 cut(s)	1089
BstV1I	GCAGC	3 cut(s)	340, 950, 1006
BstXI	CCANNNNNNTGG	1 cut(s)	681
BsuI	GTATCC	2 cut(s)	205, 1120
BsuRI	GGCC	2 cut(s)	483, 1048
BtgI	CCRYGG	1 cut(s)	82
BtsCI	GGATG	2 cut(s)	350, 708
BtsI	GCAGTG	1 cut(s)	802
BtsIMutI	CAGTG	3 cut(s)	154, 802, 975
Cac8I	GCNNGC	2 cut(s)	360, 629
CaiI	CAGNNNCTG	1 cut(s)	872
Cfr13I	GGNCC	1 cut(s)	1046
Csp6I	GTAC	3 cut(s)	432, 724, 850
CviAII	CATG	5 cut(s)	83, 332, 820, 860, 1252
CviQI	GTAC	3 cut(s)	432, 724, 850
DdeI	CTNAG	4 cut(s)	246, 317, 424, 639
DpnI	GATC	5 cut(s)	135, 251, 540, 591, 672
DpnII	GATC	5 cut(s)	133, 249, 538, 589, 670
DraI	TTTAAA	1 cut(s)	1039
EaeI	YGGCCR	1 cut(s)	481
Eam1104I	CTCTTC	1 cut(s)	1226
EarI	CTCTTC	1 cut(s)	1226
Ecl136II	GAGCTC	1 cut(s)	202
Eco130I	CCWWGG	1 cut(s)	82
Eco24I	GRGCYC	1 cut(s)	204
Eco31I	GGTCTC	1 cut(s)	498
Eco53kI	GAGCTC	1 cut(s)	202
EcoICRI	GAGCTC	1 cut(s)	202
EcoRII	CCWGG	2 cut(s)	607, 852
EcoT14I	CCWWGG	1 cut(s)	82
EcoT38I	GRGCYC	1 cut(s)	204
ErhI	CCWWGG	1 cut(s)	82
FaeI	CATG	5 cut(s)	86, 335, 823, 863, 1255
FaqI	GGGAC	1 cut(s)	877
FatI	CATG	5 cut(s)	82, 331, 819, 859, 1251
FauNDI	CATATG	1 cut(s)	595
Fnu4HI	GCNGC	3 cut(s)	329, 939, 1020
FokI	GGATG	2 cut(s)	337, 715
FriOI	GRGCYC	1 cut(s)	204
Fsp4HI	GCNGC	3 cut(s)	329, 939, 1020
FspBI	CTAG	2 cut(s)	1187, 1287
GluI	GCNGC	3 cut(s)	329, 939, 1020
GsaI	CCCAGC	1 cut(s)	419
GsuI	CTGGAG	1 cut(s)	130
HaeIII	GGCC	2 cut(s)	483, 1048
Hin1II	CATG	5 cut(s)	86, 335, 823, 863, 1255
HindIII	AAGCTT	1 cut(s)	66
HinfI	GANTC	2 cut(s)	309, 369
HphI	GGTGA	4 cut(s)	91, 109, 904, 1073
Hpy166II	GTNNAC	1 cut(s)	488
Hpy188I	TCNGA	4 cut(s)	273, 355, 642, 1316
Hpy188III	TCNNGA	7 cut(s)	382, 448, 682, 948, 1127, 1144, 1263
Hpy8I	GTNNAC	1 cut(s)	488
HpyAV	CCTTC	8 cut(s)	23, 117, 270, 323, 330, 574, 1000, 1124
HpyCH4III	ACNGT	3 cut(s)	396, 431, 548
HpyCH4V	TGCA	6 cut(s)	362, 802, 819, 1022, 1195, 1310
HpyF10VI	GCNNNNNNNGC	3 cut(s)	251, 364, 1054
HpyF3I	CTNAG	4 cut(s)	246, 317, 424, 639
Hsp92II	CATG	5 cut(s)	86, 335, 823, 863, 1255
KpnI	GGTACC	1 cut(s)	853
Kzo9I	GATC	5 cut(s)	133, 249, 538, 589, 670
LguI	GCTCTTC	1 cut(s)	1226
LmnI	GCTCC	4 cut(s)	340, 555, 622, 935
Lsp1109I	GCAGC	3 cut(s)	340, 950, 1006
MaeI	CTAG	2 cut(s)	1187, 1287
MaeIII	GTNAC	3 cut(s)	60, 259, 985
MalI	GATC	5 cut(s)	135, 251, 540, 591, 672
MboI	GATC	5 cut(s)	133, 249, 538, 589, 670
MboII	GAAGA	4 cut(s)	176, 797, 1180, 1213
MfeI	CAATTG	1 cut(s)	1293
MhlI	GDGCHC	2 cut(s)	204, 1089
MluCI	AATT	9 cut(s)	168, 216, 302, 550, 1032, 1040, 1066, 1266, 1293
MroXI	GAANNNNTTC	1 cut(s)	396
MseI	TTAA	6 cut(s)	171, 219, 476, 687, 1038, 1244
MslI	CAYNNNNRTG	1 cut(s)	147
MspR9I	CCNGG	2 cut(s)	609, 854
MunI	CAATTG	1 cut(s)	1293
Mva1269I	GAATGC	1 cut(s)	1197
MvaI	CCWGG	2 cut(s)	609, 854
MwoI	GCNNNNNNNGC	3 cut(s)	251, 364, 1054
NcoI	CCATGG	1 cut(s)	82
NdeI	CATATG	1 cut(s)	595
NdeII	GATC	5 cut(s)	133, 249, 538, 589, 670
NlaIII	CATG	5 cut(s)	86, 335, 823, 863, 1255
NlaIV	GGNNCC	5 cut(s)	191, 462, 851, 900, 1047
NspV	TTCGAA	1 cut(s)	307
OliI	CACNNNNGTG	1 cut(s)	147
PciSI	GCTCTTC	1 cut(s)	1226
PctI	GAATGC	1 cut(s)	1197
PdmI	GAANNNNTTC	1 cut(s)	396
PfeI	GAWTC	2 cut(s)	309, 369
Pfl23II	CGTACG	1 cut(s)	723
PfoI	TCCNGGA	1 cut(s)	607
PkrI	GCNGC	3 cut(s)	330, 940, 1021
PshBI	ATTAAT	1 cut(s)	687
Psp124BI	GAGCTC	1 cut(s)	204
Psp6I	CCWGG	2 cut(s)	607, 852
PspFI	CCCAGC	1 cut(s)	415
PspGI	CCWGG	2 cut(s)	607, 852
PspLI	CGTACG	1 cut(s)	723
PspN4I	GGNNCC	5 cut(s)	191, 462, 851, 900, 1047
PspPI	GGNCC	1 cut(s)	1046
PsrI	GAACNNNNNNTAC	2 cut(s)	384, 416
PstNI	CAGNNNCTG	1 cut(s)	872
RsaI	GTAC	3 cut(s)	433, 725, 851
RsaNI	GTAC	3 cut(s)	432, 724, 850
RseI	CAYNNNNRTG	1 cut(s)	147
SacI	GAGCTC	1 cut(s)	204
SapI	GCTCTTC	1 cut(s)	1226
SaqAI	TTAA	6 cut(s)	171, 219, 476, 687, 1038, 1244
SatI	GCNGC	3 cut(s)	329, 939, 1020
Sau3AI	GATC	5 cut(s)	133, 249, 538, 589, 670
Sau96I	GGNCC	1 cut(s)	1046
ScrFI	CCNGG	2 cut(s)	609, 854
SduI	GDGCHC	2 cut(s)	204, 1089
SfcI	CTRYAG	1 cut(s)	265
SfuI	TTCGAA	1 cut(s)	307
SmiMI	CAYNNNNRTG	1 cut(s)	147
SmlI	CTYRAG	1 cut(s)	382
SmoI	CTYRAG	1 cut(s)	382
Sse9I	AATT	9 cut(s)	168, 216, 302, 550, 1032, 1040, 1066, 1266, 1293
SspMI	CTAG	2 cut(s)	1187, 1287
SstI	GAGCTC	1 cut(s)	204
StyD4I	CCNGG	2 cut(s)	607, 852
StyI	CCWWGG	1 cut(s)	82
TaaI	ACNGT	3 cut(s)	396, 431, 548
TaqI	TCGA	3 cut(s)	307, 885, 1143
TasI	AATT	9 cut(s)	168, 216, 302, 550, 1032, 1040, 1066, 1266, 1293
TfiI	GAWTC	2 cut(s)	309, 369
Tru1I	TTAA	6 cut(s)	171, 219, 476, 687, 1038, 1244
Tru9I	TTAA	6 cut(s)	171, 219, 476, 687, 1038, 1244
TscAI	CASTG	3 cut(s)	154, 809, 982
TseI	GCWGC	3 cut(s)	328, 938, 1019
TspDTI	ATGAA	3 cut(s)	785, 1023, 1240
TspGWI	ACGGA	1 cut(s)	664
TspRI	CASTG	3 cut(s)	154, 809, 982
VspI	ATTAAT	1 cut(s)	687
XapI	RAATTY	2 cut(s)	302, 1266
XmnI	GAANNNNTTC	1 cut(s)	396
XspI	CTAG	2 cut(s)	1187, 1287

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.