FvH4_3g04590

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007660
Physical Location & Seq
Reverse (-)
2627189 .. 2628148
960 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04590.t1

Sequence Viewer

Length: 960 bp
ATGGAGGAAGAGAGGAGGAGGCAGCCCTGGCCTCACTACTGGGGCACCACACCGGAGCAAGACTACTACACTTCACAGGGGATCAAATCCTCCAAAGCCTTCTTCACCTCCCCCAGAGGACTCAAGCTCTTCACACGCCAATGGCTCCCTCTTCTCTCTCCTCCACGTGGCCTCATCTTCATGGTCCACGGCTACGGCAACGACATCAGCTGGACCTTTCAGGCCACCGCCGTCTTCCTCGCCCGTCACGGCTTCGCCTGCTTCGCTCTCGACCTCGAGTCCCACGGCGGCTCCCAAGGCCTCCGCGCCTTCGTCCCCAACGTCGATCTCGTCGTCGCCGACTGCCGCTTCTTCTTCAGCCTCGTCAAGCGGAGCCCCGGTTTCGAAACCCTACCGTCTTTTCTCTACGGCGAGTCCATGGGCGGCGCGATCTGTCTTCTGATCCACTTCGCGGATCCGGAAGCCTTCGACGGCGCGGTTCTGGTGGCGCCGATGTGTAAAATCTCCGACGACGTCAAGCCCAAGTGGCCGATCCCTCAGATCCTGACGTTCGTCGCCAAGTTCCTGCCGACTTTGGCGATTGTGCCCACCGGGGACCTGATGGTAAAATCGGTCAAGGTCGAGGAGAAAAAGGTAATTGCGGACATGAACCCTTCCAGGTATAGAGGGAGGCCTAGATTGGGTACAGTCATGGAGCTACTGAGAGTGACGGACTATGTCAGCCAGAGGCTGGGAGATGTGAGGCTGCCGTTTATTGTGCTCCACGGCAGCGCCGACGTGGTGACGGATCCCAATGTGAGTAGAGCTTTGTATGAAGAAGCAAAGAGTGAAGATAAGACGATCAAGATTTACGACGGGATGATGCATTCCTTGCTGTTCGGGGAGACTGATCAGAACGTCGAAATCGTCCGCAGCGACATTCTGGCATGGTTGAACCAGAGATGCACAGCAAGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

320

Amino Acids

35.97

Weight (kDa)

7.67

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 54 - 295 2.8e-55 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 59 - 293 6.5e-12 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 516
AccB1I GGYRCC 2 cut(s) 44, 487
AccB7I CCANNNNNTGG 1 cut(s) 730
AccII CGCG 4 cut(s) 306, 428, 452, 476
AccIII TCCGGA 1 cut(s) 457
AclWI GGATC 8 cut(s) 89, 436, 449, 462, 526, 535, 782, 795
AcoI YGGCCR 1 cut(s) 527
AcuI CTGAAG 1 cut(s) 340
AcvI CACGTG 1 cut(s) 167
AcyI GRCGYC 2 cut(s) 488, 513
AfaI GTAC 1 cut(s) 685
AfiI CCNNNNNNNGG 4 cut(s) 167, 451, 680, 730
AgsI TTSAA 1 cut(s) 934
AhdI GACNNNNNGTC 1 cut(s) 277
AjiI CACGTC 1 cut(s) 778
AjnI CCWGG 2 cut(s) 26, 656
AjuI GAANNNNNNNTTGG 2 cut(s) 86, 118
AluBI AGCT 4 cut(s) 127, 210, 697, 806
AluI AGCT 4 cut(s) 127, 210, 697, 806
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 1 cut(s) 878
AlwI GGATC 8 cut(s) 89, 436, 449, 462, 526, 535, 782, 795
AlwNI CAGNNNCTG 2 cut(s) 544, 730
Ama87I CYCGRG 1 cut(s) 275
Aor13HI TCCGGA 1 cut(s) 457
AoxI GGCC 6 cut(s) 29, 169, 222, 298, 527, 671
ApeKI GCWGC 4 cut(s) 22, 745, 768, 912
AspLEI GCGC 5 cut(s) 308, 428, 476, 490, 773
AspS9I GGNCC 3 cut(s) 184, 213, 595
AsuC2I CCSGG 2 cut(s) 378, 592
AsuHPI GGTGA 2 cut(s) 97, 793
AsuII TTCGAA 1 cut(s) 384
AvaI CYCGRG 1 cut(s) 275
AvaII GGWCC 3 cut(s) 184, 213, 595
BaeGI GKGCMC 2 cut(s) 47, 588
BamHI GGATCC 2 cut(s) 454, 787
BanI GGYRCC 2 cut(s) 44, 487
BanII GRGCYC 1 cut(s) 377
BbrPI CACGTG 1 cut(s) 167
BbsI GAAGAC 2 cut(s) 226, 428
Bbv12I GWGCWC 1 cut(s) 762
BbvI GCAGC 4 cut(s) 34, 732, 780, 924
BccI CCATC 1 cut(s) 595
BceAI ACGGC 9 cut(s) 205, 211, 215, 265, 301, 424, 487, 733, 781
BciT130I CCWGG 2 cut(s) 28, 658
BclI TGATCA 1 cut(s) 889
BcnI CCSGG 2 cut(s) 378, 592
BcoDI GTCTC 1 cut(s) 878
BfaI CTAG 1 cut(s) 675
BfoI RGCGCY 2 cut(s) 491, 774
BglI GCCNNNNNGGC 1 cut(s) 526
BisI GCNGC 7 cut(s) 23, 289, 346, 424, 746, 769, 913
BlsI GCNGC 7 cut(s) 24, 290, 347, 425, 747, 770, 914
Bme1390I CCNGG 4 cut(s) 28, 378, 592, 658
Bme18I GGWCC 3 cut(s) 184, 213, 595
BmeRI GACNNNNNGTC 1 cut(s) 277
BmeT110I CYCGRG 1 cut(s) 275
BmgBI CACGTC 1 cut(s) 778
BmgT120I GGNCC 3 cut(s) 184, 213, 595
BmiI GGNNCC 8 cut(s) 46, 146, 292, 374, 456, 489, 596, 789
BmrFI CCNGG 4 cut(s) 28, 378, 592, 658
BmrI ACTGGG 1 cut(s) 49
BmsI GCATC 2 cut(s) 852, 932
BmuI ACTGGG 1 cut(s) 49
BoxI GACNNNNGTC 1 cut(s) 551
BpiI GAAGAC 2 cut(s) 226, 428
Bpu14I TTCGAA 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 2 cut(s) 378, 592
BsaAI YACGTR 1 cut(s) 167
BsaHI GRCGYC 2 cut(s) 488, 513
BsaJI CCNNGG 8 cut(s) 26, 187, 283, 295, 376, 417, 591, 763
BsaWI WCCGGW 2 cut(s) 52, 457
BsaXI ACNNNNNCTCC 4 cut(s) 275, 305, 364, 394
Bsc4I CCNNNNNNNGG 4 cut(s) 167, 451, 680, 730
Bse1I ACTGG 1 cut(s) 44
BseAI TCCGGA 1 cut(s) 457
BseBI CCWGG 2 cut(s) 28, 658
BseDI CCNNGG 8 cut(s) 26, 187, 283, 295, 376, 417, 591, 763
BseGI GGATG 1 cut(s) 864
BseLI CCNNNNNNNGG 4 cut(s) 167, 451, 680, 730
BseMII CTCAG 2 cut(s) 551, 692
BseNI ACTGG 1 cut(s) 44
BseRI GAGGAG 4 cut(s) 28, 31, 150, 638
BseSI GKGCMC 2 cut(s) 47, 588
BseXI GCAGC 4 cut(s) 34, 732, 780, 924
BseYI CCCAGC 1 cut(s) 730
Bsh1236I CGCG 4 cut(s) 306, 428, 452, 476
BshFI GGCC 6 cut(s) 31, 171, 224, 300, 529, 673
BshNI GGYRCC 2 cut(s) 44, 487
BsiHKAI GWGCWC 1 cut(s) 762
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 4 cut(s) 53, 378, 458, 591
BslFI GGGAC 3 cut(s) 265, 299, 608
BslI CCNNNNNNNGG 4 cut(s) 167, 451, 680, 730
BsmAI GTCTC 1 cut(s) 878
BsmFI GGGAC 3 cut(s) 265, 299, 608
BsmI GAATGC 1 cut(s) 865
BsnI GGCC 6 cut(s) 31, 171, 224, 300, 529, 673
BsoBI CYCGRG 1 cut(s) 275
Bsp119I TTCGAA 1 cut(s) 384
Bsp1286I GDGCHC 4 cut(s) 47, 377, 588, 762
Bsp13I TCCGGA 1 cut(s) 457
Bsp19I CCATGG 1 cut(s) 417
BspANI GGCC 6 cut(s) 31, 171, 224, 300, 529, 673
BspCNI CTCAG 2 cut(s) 550, 693
BspEI TCCGGA 1 cut(s) 457
BspFNI CGCG 4 cut(s) 306, 428, 452, 476
BspLI GGNNCC 8 cut(s) 46, 146, 292, 374, 456, 489, 596, 789
BspPI GGATC 8 cut(s) 89, 436, 449, 462, 526, 535, 782, 795
BspQI GCTCTTC 1 cut(s) 134
BspT104I TTCGAA 1 cut(s) 384
BspT107I GGYRCC 2 cut(s) 44, 487
BsrI ACTGG 1 cut(s) 44
BssECI CCNNGG 8 cut(s) 26, 187, 283, 295, 376, 417, 591, 763
BssNI GRCGYC 2 cut(s) 488, 513
BssT1I CCWWGG 2 cut(s) 295, 417
Bst2UI CCWGG 2 cut(s) 28, 658
Bst4CI ACNGT 2 cut(s) 396, 688
Bst6I CTCTTC 3 cut(s) 3, 134, 156
BstACI GRCGYC 2 cut(s) 488, 513
BstAPI GCANNNNNTGC 1 cut(s) 871
BstBAI YACGTR 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 384
BstC8I GCNNGC 1 cut(s) 259
BstDEI CTNAG 2 cut(s) 537, 701
BstDSI CCRYGG 4 cut(s) 187, 283, 417, 763
BstF5I GGATG 1 cut(s) 864
BstFNI CGCG 4 cut(s) 306, 428, 452, 476
BstH2I RGCGCY 2 cut(s) 491, 774
BstHHI GCGC 5 cut(s) 308, 428, 476, 490, 773
BstMAI GTCTC 1 cut(s) 878
BstMWI GCNNNNNNNGC 6 cut(s) 28, 258, 263, 297, 526, 871
BstNI CCWGG 2 cut(s) 28, 658
BstPAI GACNNNNGTC 1 cut(s) 551
BstSCI CCNGG 4 cut(s) 26, 376, 590, 656
BstSLI GKGCMC 2 cut(s) 47, 588
BstUI CGCG 4 cut(s) 306, 428, 452, 476
BstV1I GCAGC 4 cut(s) 34, 732, 780, 924
BstV2I GAAGAC 2 cut(s) 226, 428
BstX2I RGATCY 3 cut(s) 454, 540, 787
BstYI RGATCY 3 cut(s) 454, 540, 787
BsuRI GGCC 6 cut(s) 31, 171, 224, 300, 529, 673
BtgI CCRYGG 4 cut(s) 187, 283, 417, 763
BtrI CACGTC 1 cut(s) 778
BtsCI GGATG 1 cut(s) 864
Cac8I GCNNGC 1 cut(s) 259
CaiI CAGNNNCTG 2 cut(s) 544, 730
CfoI GCGC 5 cut(s) 308, 428, 476, 490, 773
Cfr13I GGNCC 3 cut(s) 184, 213, 595
Csp6I GTAC 1 cut(s) 684
CviAII CATG 5 cut(s) 181, 418, 646, 691, 927
CviQI GTAC 1 cut(s) 684
DdeI CTNAG 2 cut(s) 537, 701
DinI GGCGCC 1 cut(s) 489
DriI GACNNNNNGTC 1 cut(s) 277
EaeI YGGCCR 1 cut(s) 527
Eam1104I CTCTTC 3 cut(s) 3, 134, 156
Eam1105I GACNNNNNGTC 1 cut(s) 277
EarI CTCTTC 3 cut(s) 3, 134, 156
Eco130I CCWWGG 2 cut(s) 295, 417
Eco147I AGGCCT 2 cut(s) 300, 673
Eco24I GRGCYC 1 cut(s) 377
Eco47I GGWCC 3 cut(s) 184, 213, 595
Eco57I CTGAAG 1 cut(s) 340
Eco72I CACGTG 1 cut(s) 167
Eco88I CYCGRG 1 cut(s) 275
EcoO109I RGGNCCY 1 cut(s) 595
EcoRII CCWGG 2 cut(s) 26, 656
EcoT14I CCWWGG 2 cut(s) 295, 417
EcoT22I ATGCAT 1 cut(s) 867
EcoT38I GRGCYC 1 cut(s) 377
EgeI GGCGCC 1 cut(s) 489
EheI GGCGCC 1 cut(s) 489
ErhI CCWWGG 2 cut(s) 295, 417
FaeI CATG 5 cut(s) 184, 421, 649, 694, 930
FaiI YATR 8 cut(s) 182, 419, 647, 663, 692, 717, 813, 928
FaqI GGGAC 3 cut(s) 265, 299, 608
FatI CATG 5 cut(s) 180, 417, 645, 690, 926
FbaI TGATCA 1 cut(s) 889
Fnu4HI GCNGC 7 cut(s) 23, 289, 346, 424, 746, 769, 913
FokI GGATG 1 cut(s) 871
FriOI GRGCYC 1 cut(s) 377
Fsp4HI GCNGC 7 cut(s) 23, 289, 346, 424, 746, 769, 913
FspBI CTAG 1 cut(s) 675
GlaI GCGC 5 cut(s) 307, 427, 475, 489, 772
GluI GCNGC 7 cut(s) 23, 289, 346, 424, 746, 769, 913
GsaI CCCAGC 1 cut(s) 734
HaeII RGCGCY 2 cut(s) 491, 774
HaeIII GGCC 6 cut(s) 31, 171, 224, 300, 529, 673
HapII CCGG 4 cut(s) 53, 378, 458, 591
HhaI GCGC 5 cut(s) 308, 428, 476, 490, 773
Hin1I GRCGYC 2 cut(s) 488, 513
Hin1II CATG 5 cut(s) 184, 421, 649, 694, 930
Hin6I GCGC 5 cut(s) 306, 426, 474, 488, 771
HinP1I GCGC 5 cut(s) 306, 426, 474, 488, 771
HinfI GANTC 3 cut(s) 120, 278, 413
HpaII CCGG 4 cut(s) 53, 378, 458, 591
HphI GGTGA 2 cut(s) 97, 793
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 4 cut(s) 441, 508, 540, 894
Hpy188III TCNNGA 4 cut(s) 269, 458, 544, 844
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 4 cut(s) 109, 319, 475, 663
HpyCH4III ACNGT 2 cut(s) 396, 688
HpyCH4IV ACGT 6 cut(s) 166, 321, 513, 548, 777, 897
HpyCH4V TGCA 2 cut(s) 865, 945
HpyF10VI GCNNNNNNNGC 6 cut(s) 28, 258, 263, 297, 526, 871
HpyF3I CTNAG 2 cut(s) 537, 701
HpySE526I ACGT 6 cut(s) 166, 321, 513, 548, 777, 897
Hsp92I GRCGYC 2 cut(s) 488, 513
Hsp92II CATG 5 cut(s) 184, 421, 649, 694, 930
HspAI GCGC 5 cut(s) 306, 426, 474, 488, 771
KasI GGCGCC 1 cut(s) 487
Kpn2I TCCGGA 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 889
LguI GCTCTTC 1 cut(s) 134
LmnI GCTCC 6 cut(s) 55, 150, 296, 372, 694, 765
Lsp1109I GCAGC 4 cut(s) 34, 732, 780, 924
LweI GCATC 2 cut(s) 852, 932
MaeI CTAG 1 cut(s) 675
MaeII ACGT 6 cut(s) 166, 321, 513, 548, 777, 897
MaeIII GTNAC 3 cut(s) 245, 706, 781
MflI RGATCY 3 cut(s) 454, 540, 787
MhlI GDGCHC 4 cut(s) 47, 377, 588, 762
MluCI AATT 2 cut(s) 636, 955
Mly113I GGCGCC 1 cut(s) 488
MlyI GAGTC 3 cut(s) 114, 287, 422
MmeI TCCRAC 1 cut(s) 531
Mph1103I ATGCAT 1 cut(s) 867
MroI TCCGGA 1 cut(s) 457
MslI CAYNNNNRTG 2 cut(s) 139, 179
MspA1I CMGCKG 1 cut(s) 210
MspI CCGG 4 cut(s) 53, 378, 458, 591
MspR9I CCNGG 4 cut(s) 28, 378, 592, 658
Mva1269I GAATGC 1 cut(s) 865
MvaI CCWGG 2 cut(s) 28, 658
MvnI CGCG 4 cut(s) 306, 428, 452, 476
MwoI GCNNNNNNNGC 6 cut(s) 28, 258, 263, 297, 526, 871
NarI GGCGCC 1 cut(s) 488
NciI CCSGG 2 cut(s) 378, 592
NcoI CCATGG 1 cut(s) 417
NlaIII CATG 5 cut(s) 184, 421, 649, 694, 930
NlaIV GGNNCC 8 cut(s) 46, 146, 292, 374, 456, 489, 596, 789
NmuCI GTSAC 3 cut(s) 245, 706, 781
NsiI ATGCAT 1 cut(s) 867
NspV TTCGAA 1 cut(s) 384
PaeR7I CTCGAG 1 cut(s) 275
PceI AGGCCT 2 cut(s) 300, 673
PciSI GCTCTTC 1 cut(s) 134
PcsI WCGNNNNNNNCGW 7 cut(s) 318, 327, 330, 336, 771, 903, 912
PctI GAATGC 1 cut(s) 865
PflFI GACNNNGTC 2 cut(s) 512, 716
PflMI CCANNNNNTGG 1 cut(s) 730
PkrI GCNGC 7 cut(s) 24, 290, 347, 425, 747, 770, 914
PleI GAGTC 3 cut(s) 114, 286, 421
PluTI GGCGCC 1 cut(s) 491
PmaCI CACGTG 1 cut(s) 167
PmlI CACGTG 1 cut(s) 167
PpsI GAGTC 3 cut(s) 114, 286, 421
Ppu21I YACGTR 1 cut(s) 167
PpuMI RGGWCCY 1 cut(s) 595
PshAI GACNNNNGTC 1 cut(s) 551
Psp5II RGGWCCY 1 cut(s) 595
Psp6I CCWGG 2 cut(s) 26, 656
PspCI CACGTG 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 730
PspGI CCWGG 2 cut(s) 26, 656
PspN4I GGNNCC 8 cut(s) 46, 146, 292, 374, 456, 489, 596, 789
PspPI GGNCC 3 cut(s) 184, 213, 595
PspPPI RGGWCCY 1 cut(s) 595
PspXI VCTCGAGB 1 cut(s) 275
PstNI CAGNNNCTG 2 cut(s) 544, 730
PsuI RGATCY 3 cut(s) 454, 540, 787
PsyI GACNNNGTC 2 cut(s) 512, 716
PvuII CAGCTG 1 cut(s) 210
RsaI GTAC 1 cut(s) 685
RsaNI GTAC 1 cut(s) 684
RseI CAYNNNNRTG 2 cut(s) 139, 179
SapI GCTCTTC 1 cut(s) 134
SatI GCNGC 7 cut(s) 23, 289, 346, 424, 746, 769, 913
Sau96I GGNCC 3 cut(s) 184, 213, 595
SchI GAGTC 3 cut(s) 114, 287, 422
ScrFI CCNGG 4 cut(s) 28, 378, 592, 658
SduI GDGCHC 4 cut(s) 47, 377, 588, 762
SfaNI GCATC 2 cut(s) 852, 932
SfoI GGCGCC 1 cut(s) 489
Sfr274I CTCGAG 1 cut(s) 275
SfuI TTCGAA 1 cut(s) 384
SinI GGWCC 3 cut(s) 184, 213, 595
SlaI CTCGAG 1 cut(s) 275
SmiMI CAYNNNNRTG 2 cut(s) 139, 179
SmlI CTYRAG 2 cut(s) 122, 275
SmoI CTYRAG 2 cut(s) 122, 275
Sse9I AATT 2 cut(s) 636, 955
SseBI AGGCCT 2 cut(s) 300, 673
SspDI GGCGCC 1 cut(s) 487
SspMI CTAG 1 cut(s) 675
StuI AGGCCT 2 cut(s) 300, 673
StyD4I CCNGG 4 cut(s) 26, 376, 590, 656
StyI CCWWGG 2 cut(s) 295, 417
TaaI ACNGT 2 cut(s) 396, 688
TaiI ACGT 6 cut(s) 169, 324, 516, 551, 780, 900
TaqI TCGA 7 cut(s) 270, 276, 324, 384, 468, 621, 900
TaqII GACCGA 1 cut(s) 601
TasI AATT 2 cut(s) 636, 955
TauI GCSGC 3 cut(s) 291, 348, 426
TseFI GTSAC 3 cut(s) 245, 706, 781
TseI GCWGC 4 cut(s) 22, 745, 768, 912
Tsp45I GTSAC 3 cut(s) 245, 706, 781
TspDTI ATGAA 3 cut(s) 169, 662, 828
TspGWI ACGGA 2 cut(s) 725, 800
Tth111I GACNNNGTC 2 cut(s) 512, 716
Van91I CCANNNNNTGG 1 cut(s) 730
VpaK11BI GGWCC 3 cut(s) 184, 213, 595
XhoI CTCGAG 1 cut(s) 275
XspI CTAG 1 cut(s) 675
ZraI GACGTC 1 cut(s) 514
Zsp2I ATGCAT 1 cut(s) 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.