pycom10g25280

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007660
Physical Location & Seq
Forward (+)
26963902 .. 26964933
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g25280.1

Sequence Viewer

Length: 1032 bp
ATGGAGGAGCTAGAAAGCGTCACCACCACCAGCCGGACCCAATCGGGCCAACCCGAATCCCAACCCCGAGCCCAGCTCCCCCACTACTGGGGCACCACACCAGAGCAAGAATACTACAATCTCCAGGGCATCAAATCCTCCAAATCCTTCTACGCCCCCCCCAGAGGCCTTAAGCTCTTCACCCGCCAATGGCTCCCCCTCCGCCGTCCACGTGGCATCATCTTCATGGTCCACGGGTACGGCAACGACATCAGCTGGACCTTCCAAGCCACCCCGATCTTCCTCGCCCAAAACGGTTTTGCCTGCTTCGCTCTCGACCTCGAGGGCCACGGTGGCTCCGACGGCCTCCGCGCCTTCGTCCCCGACGTTGGCCTCGTGGTCAACGACTGCCTCTCGTTTATCAATTTGGTCAAACACCAGGACCCCCAATTTCAGGGACTCCCCTGTTTCTTATACGGCGAGTCCATGGGCGGCGCGATTTGCCTCCTCATCCACTTCGCCGCCCCGGAGGCGTTCCAAGGCGCGATCTTGGCTGCCCCCATGTGTAAAATCTCGGATAAAGTAAAACCCAGATGGCCGATTCCGGAAATTCTGACATTCGTGGCGAGATACTTGCCGAGATTGGCGATCGTCCCGACCGCCGACCTGATGCGGAAGTCCGTTAAGGTGGAGGAGAAGAAGATCGTGGCGGATATGAATCCGTCGAGATACCGGGGGAAGCCGAGGCTGGGGACGGTGGTGGAACTGCTGAGAGTGACGCAGTACGTGAGCGAGAGATTGGGGGATGTGAATCTTCCGTTTATTGTGTTGCACGGCAGCGCGGATGTGGTGACGGACCCGGAGGTGAGCATGGCGTTGTATGCGGCGGCGAAGAGCGAGGATAAGACGTTGAAGATTTATGAGGGGATGATGCATTCGCTGCTGTTTGGCGAGACTGATGAGAATGTGGAGGTTGTGAGGGGAGATATCTTGGGTTGGTTGAATCATCGGTGCGGAGGAGGCGGCGGCGGCGGCGGCGAAAGAGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

344

Amino Acids

37.99

Weight (kDa)

6.27

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 69 - 311 7.2e-57 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 74 - 309 1.5e-12 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 74 - 187 8.8e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 320
AccB1I GGYRCC 1 cut(s) 92
AccII CGCG 4 cut(s) 351, 476, 524, 821
AccIII TCCGGA 1 cut(s) 583
AcoI YGGCCR 1 cut(s) 575
AcsI RAATTY 1 cut(s) 588
AcvI CACGTG 1 cut(s) 212
AfaI GTAC 2 cut(s) 239, 764
AfiI CCNNNNNNNGG 8 cut(s) 33, 87, 88, 164, 189, 368, 433, 728
AflII CTTAAG 1 cut(s) 170
AgsI TTSAA 2 cut(s) 892, 982
AjnI CCWGG 2 cut(s) 123, 417
AluBI AGCT 4 cut(s) 10, 76, 175, 255
AluI AGCT 4 cut(s) 10, 76, 175, 255
Alw26I GTCTC 1 cut(s) 926
Ama87I CYCGRG 2 cut(s) 66, 320
Aor13HI TCCGGA 1 cut(s) 583
AoxI GGCC 6 cut(s) 46, 166, 325, 343, 370, 575
ApeKI GCWGC 3 cut(s) 533, 816, 919
ApoI RAATTY 1 cut(s) 588
AspLEI GCGC 4 cut(s) 353, 476, 524, 821
AspS9I GGNCC 7 cut(s) 36, 46, 229, 258, 325, 421, 835
AsuC2I CCSGG 3 cut(s) 506, 713, 839
AsuHPI GGTGA 4 cut(s) 13, 172, 841, 856
AvaI CYCGRG 2 cut(s) 66, 320
AvaII GGWCC 5 cut(s) 36, 229, 258, 421, 835
BaeGI GKGCMC 1 cut(s) 95
BanI GGYRCC 1 cut(s) 92
BanII GRGCYC 1 cut(s) 73
BauI CACGAG 1 cut(s) 374
BbrPI CACGTG 1 cut(s) 212
BbvI GCAGC 3 cut(s) 520, 828, 906
BccI CCATC 1 cut(s) 567
BceAI ACGGC 5 cut(s) 189, 256, 358, 472, 829
BcgI CGANNNNNNTGC 2 cut(s) 595, 629
BciT130I CCWGG 2 cut(s) 125, 419
BcnI CCSGG 3 cut(s) 506, 713, 839
BcoDI GTCTC 1 cut(s) 926
BfaI CTAG 1 cut(s) 11
BfrI CTTAAG 1 cut(s) 170
BglI GCCNNNNNGGC 2 cut(s) 333, 509
Bme1390I CCNGG 5 cut(s) 125, 419, 506, 713, 839
Bme18I GGWCC 5 cut(s) 36, 229, 258, 421, 835
BmeT110I CYCGRG 2 cut(s) 66, 320
BmgT120I GGNCC 7 cut(s) 36, 46, 229, 258, 325, 421, 835
BmiI GGNNCC 6 cut(s) 38, 94, 194, 337, 423, 837
BmrFI CCNGG 5 cut(s) 125, 419, 506, 713, 839
BmrI ACTGGG 1 cut(s) 97
BmsI GCATC 4 cut(s) 138, 225, 639, 900
BmuI ACTGGG 1 cut(s) 97
BplI GAGNNNNNCTC 2 cut(s) 60, 92
BpmI CTGGAG 1 cut(s) 107
BpuMI CCSGG 3 cut(s) 506, 713, 839
BsaAI YACGTR 2 cut(s) 212, 766
BsaBI GATNNNNATC 2 cut(s) 696, 789
BsaJI CCNNGG 8 cut(s) 124, 232, 328, 465, 504, 517, 712, 722
BsaWI WCCGGW 1 cut(s) 583
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 8 cut(s) 33, 87, 88, 164, 189, 368, 433, 728
Bse1I ACTGG 1 cut(s) 92
Bse8I GATNNNNATC 2 cut(s) 696, 789
BseAI TCCGGA 1 cut(s) 583
BseBI CCWGG 2 cut(s) 125, 419
BseDI CCNNGG 8 cut(s) 124, 232, 328, 465, 504, 517, 712, 722
BseGI GGATG 4 cut(s) 489, 790, 829, 912
BseJI GATNNNNATC 2 cut(s) 696, 789
BseLI CCNNNNNNNGG 8 cut(s) 33, 87, 88, 164, 189, 368, 433, 728
BseMII CTCAG 1 cut(s) 740
BseNI ACTGG 1 cut(s) 92
BseRI GAGGAG 4 cut(s) 20, 476, 686, 1011
BseSI GKGCMC 1 cut(s) 95
BseXI GCAGC 3 cut(s) 520, 828, 906
BseYI CCCAGC 2 cut(s) 72, 727
Bsh1236I CGCG 4 cut(s) 351, 476, 524, 821
Bsh1285I CGRYCG 2 cut(s) 630, 639
BshFI GGCC 6 cut(s) 48, 168, 327, 345, 372, 577
BshNI GGYRCC 1 cut(s) 92
BsiEI CGRYCG 2 cut(s) 630, 639
BsiHKCI CYCGRG 2 cut(s) 66, 320
BsiSI CCGG 5 cut(s) 34, 506, 584, 712, 839
BslFI GGGAC 4 cut(s) 344, 450, 617, 745
BslI CCNNNNNNNGG 8 cut(s) 33, 87, 88, 164, 189, 368, 433, 728
BsmAI GTCTC 1 cut(s) 926
BsmFI GGGAC 4 cut(s) 344, 450, 617, 745
BsmI GAATGC 1 cut(s) 913
BsnI GGCC 6 cut(s) 48, 168, 327, 345, 372, 577
BsoBI CYCGRG 2 cut(s) 66, 320
Bsp1286I GDGCHC 2 cut(s) 73, 95
Bsp13I TCCGGA 1 cut(s) 583
Bsp143I GATC 4 cut(s) 276, 525, 627, 681
Bsp19I CCATGG 1 cut(s) 465
BspANI GGCC 6 cut(s) 48, 168, 327, 345, 372, 577
BspCNI CTCAG 1 cut(s) 741
BspEI TCCGGA 1 cut(s) 583
BspFNI CGCG 4 cut(s) 351, 476, 524, 821
BspLI GGNNCC 6 cut(s) 38, 94, 194, 337, 423, 837
BspQI GCTCTTC 2 cut(s) 182, 866
BspT107I GGYRCC 1 cut(s) 92
BspTI CTTAAG 1 cut(s) 170
BsrI ACTGG 1 cut(s) 92
BssECI CCNNGG 8 cut(s) 124, 232, 328, 465, 504, 517, 712, 722
BssMI GATC 4 cut(s) 276, 525, 627, 681
BssSI CACGAG 1 cut(s) 374
BssT1I CCWWGG 2 cut(s) 465, 517
Bst2BI CACGAG 1 cut(s) 374
Bst2UI CCWGG 2 cut(s) 125, 419
Bst4CI ACNGT 3 cut(s) 296, 332, 736
Bst6I CTCTTC 2 cut(s) 182, 866
BstAFI CTTAAG 1 cut(s) 170
BstAPI GCANNNNNTGC 1 cut(s) 919
BstBAI YACGTR 2 cut(s) 212, 766
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 1 cut(s) 749
BstDSI CCRYGG 3 cut(s) 232, 328, 465
BstF5I GGATG 4 cut(s) 489, 790, 829, 912
BstFNI CGCG 4 cut(s) 351, 476, 524, 821
BstHHI GCGC 4 cut(s) 353, 476, 524, 821
BstKTI GATC 4 cut(s) 279, 528, 630, 684
BstMAI GTCTC 1 cut(s) 926
BstMBI GATC 4 cut(s) 276, 525, 627, 681
BstMCI CGRYCG 2 cut(s) 630, 639
BstNI CCWGG 2 cut(s) 125, 419
BstSCI CCNGG 5 cut(s) 123, 417, 504, 711, 837
BstSLI GKGCMC 1 cut(s) 95
BstUI CGCG 4 cut(s) 351, 476, 524, 821
BstV1I GCAGC 3 cut(s) 520, 828, 906
BsuRI GGCC 6 cut(s) 48, 168, 327, 345, 372, 577
BtgI CCRYGG 3 cut(s) 232, 328, 465
BtsCI GGATG 4 cut(s) 489, 790, 829, 912
Cac8I GCNNGC 1 cut(s) 304
CfoI GCGC 4 cut(s) 353, 476, 524, 821
Cfr13I GGNCC 7 cut(s) 36, 46, 229, 258, 325, 421, 835
CseI GACGC 2 cut(s) 7, 766
Csp6I GTAC 2 cut(s) 238, 763
CviAII CATG 4 cut(s) 226, 466, 541, 850
CviQI GTAC 2 cut(s) 238, 763
DdeI CTNAG 1 cut(s) 749
DpnI GATC 4 cut(s) 278, 527, 629, 683
DpnII GATC 4 cut(s) 276, 525, 627, 681
EaeI YGGCCR 1 cut(s) 575
Eam1104I CTCTTC 2 cut(s) 182, 866
EarI CTCTTC 2 cut(s) 182, 866
EciI GGCGGA 2 cut(s) 191, 704
Eco130I CCWWGG 2 cut(s) 465, 517
Eco147I AGGCCT 1 cut(s) 168
Eco24I GRGCYC 1 cut(s) 73
Eco32I GATATC 1 cut(s) 967
Eco47I GGWCC 5 cut(s) 36, 229, 258, 421, 835
Eco72I CACGTG 1 cut(s) 212
Eco88I CYCGRG 2 cut(s) 66, 320
EcoO109I RGGNCCY 1 cut(s) 421
EcoRII CCWGG 2 cut(s) 123, 417
EcoRV GATATC 1 cut(s) 967
EcoT14I CCWWGG 2 cut(s) 465, 517
EcoT22I ATGCAT 1 cut(s) 915
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 2 cut(s) 465, 517
FaeI CATG 4 cut(s) 229, 469, 544, 853
FaiI YATR 8 cut(s) 227, 454, 467, 542, 695, 851, 861, 900
FaqI GGGAC 4 cut(s) 344, 450, 617, 745
FatI CATG 4 cut(s) 225, 465, 540, 849
FauI CCCGC 1 cut(s) 191
FokI GGATG 4 cut(s) 476, 797, 836, 919
FriOI GRGCYC 1 cut(s) 73
FspBI CTAG 1 cut(s) 11
GlaI GCGC 4 cut(s) 352, 475, 523, 820
GsaI CCCAGC 2 cut(s) 76, 731
GsuI CTGGAG 1 cut(s) 107
HaeIII GGCC 6 cut(s) 48, 168, 327, 345, 372, 577
HapII CCGG 5 cut(s) 34, 506, 584, 712, 839
HgaI GACGC 2 cut(s) 7, 766
HhaI GCGC 4 cut(s) 353, 476, 524, 821
Hin1II CATG 4 cut(s) 229, 469, 544, 853
Hin6I GCGC 4 cut(s) 351, 474, 522, 819
HinP1I GCGC 4 cut(s) 351, 474, 522, 819
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 7 cut(s) 56, 438, 461, 580, 697, 790, 982
HpaII CCGG 5 cut(s) 34, 506, 584, 712, 839
HphI GGTGA 4 cut(s) 13, 172, 841, 856
Hpy166II GTNNAC 3 cut(s) 209, 232, 382
Hpy188I TCNGA 3 cut(s) 340, 556, 594
Hpy188III TCNNGA 4 cut(s) 314, 584, 634, 705
Hpy8I GTNNAC 3 cut(s) 209, 232, 382
Hpy99I CGWCG 3 cut(s) 344, 368, 706
HpyAV CCTTC 3 cut(s) 157, 271, 364
HpyCH4III ACNGT 3 cut(s) 296, 332, 736
HpyCH4IV ACGT 4 cut(s) 211, 366, 765, 887
HpyCH4V TGCA 2 cut(s) 811, 913
HpyF3I CTNAG 1 cut(s) 749
HpySE526I ACGT 4 cut(s) 211, 366, 765, 887
Hsp92II CATG 4 cut(s) 229, 469, 544, 853
HspAI GCGC 4 cut(s) 351, 474, 522, 819
Kpn2I TCCGGA 1 cut(s) 583
Kzo9I GATC 4 cut(s) 276, 525, 627, 681
LguI GCTCTTC 2 cut(s) 182, 866
LmnI GCTCC 4 cut(s) 7, 81, 198, 341
Lsp1109I GCAGC 3 cut(s) 520, 828, 906
LweI GCATC 4 cut(s) 138, 225, 639, 900
MaeI CTAG 1 cut(s) 11
MaeII ACGT 4 cut(s) 211, 366, 765, 887
MaeIII GTNAC 3 cut(s) 19, 754, 829
MalI GATC 4 cut(s) 278, 527, 629, 683
MboI GATC 4 cut(s) 276, 525, 627, 681
MboII GAAGA 8 cut(s) 169, 214, 271, 688, 691, 785, 883, 904
MhlI GDGCHC 2 cut(s) 73, 95
MluCI AATT 3 cut(s) 403, 428, 588
MlyI GAGTC 2 cut(s) 432, 470
MmeI TCCRAC 1 cut(s) 363
Mph1103I ATGCAT 1 cut(s) 915
MroI TCCGGA 1 cut(s) 583
MseI TTAA 2 cut(s) 171, 663
MslI CAYNNNNRTG 1 cut(s) 224
MspA1I CMGCKG 1 cut(s) 255
MspCI CTTAAG 1 cut(s) 170
MspI CCGG 5 cut(s) 34, 506, 584, 712, 839
MspR9I CCNGG 5 cut(s) 125, 419, 506, 713, 839
Mva1269I GAATGC 1 cut(s) 913
MvaI CCWGG 2 cut(s) 125, 419
MvnI CGCG 4 cut(s) 351, 476, 524, 821
NciI CCSGG 3 cut(s) 506, 713, 839
NcoI CCATGG 1 cut(s) 465
NdeII GATC 4 cut(s) 276, 525, 627, 681
NlaIII CATG 4 cut(s) 229, 469, 544, 853
NlaIV GGNNCC 6 cut(s) 38, 94, 194, 337, 423, 837
NmeAIII GCCGAG 2 cut(s) 642, 747
NmuCI GTSAC 3 cut(s) 19, 754, 829
NsiI ATGCAT 1 cut(s) 915
PaeR7I CTCGAG 1 cut(s) 320
PceI AGGCCT 1 cut(s) 168
PciSI GCTCTTC 2 cut(s) 182, 866
PcsI WCGNNNNNNNCGW 4 cut(s) 336, 363, 372, 381
PctI GAATGC 1 cut(s) 913
PfeI GAWTC 5 cut(s) 56, 580, 697, 790, 982
Ple19I CGATCG 1 cut(s) 630
PleI GAGTC 2 cut(s) 432, 469
PmaCI CACGTG 1 cut(s) 212
PmlI CACGTG 1 cut(s) 212
PpsI GAGTC 2 cut(s) 432, 469
Ppu21I YACGTR 2 cut(s) 212, 766
PpuMI RGGWCCY 1 cut(s) 421
Psp5II RGGWCCY 1 cut(s) 421
Psp6I CCWGG 2 cut(s) 123, 417
PspCI CACGTG 1 cut(s) 212
PspFI CCCAGC 2 cut(s) 72, 727
PspGI CCWGG 2 cut(s) 123, 417
PspN4I GGNNCC 6 cut(s) 38, 94, 194, 337, 423, 837
PspPI GGNCC 7 cut(s) 36, 46, 229, 258, 325, 421, 835
PspPPI RGGWCCY 1 cut(s) 421
PspXI VCTCGAGB 1 cut(s) 320
PvuI CGATCG 1 cut(s) 630
PvuII CAGCTG 1 cut(s) 255
RsaI GTAC 2 cut(s) 239, 764
RsaNI GTAC 2 cut(s) 238, 763
RseI CAYNNNNRTG 1 cut(s) 224
SapI GCTCTTC 2 cut(s) 182, 866
SaqAI TTAA 2 cut(s) 171, 663
Sau3AI GATC 4 cut(s) 276, 525, 627, 681
Sau96I GGNCC 7 cut(s) 36, 46, 229, 258, 325, 421, 835
SchI GAGTC 2 cut(s) 432, 470
ScrFI CCNGG 5 cut(s) 125, 419, 506, 713, 839
SduI GDGCHC 2 cut(s) 73, 95
SfaNI GCATC 4 cut(s) 138, 225, 639, 900
Sfr274I CTCGAG 1 cut(s) 320
SinI GGWCC 5 cut(s) 36, 229, 258, 421, 835
SlaI CTCGAG 1 cut(s) 320
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 2 cut(s) 170, 320
SmoI CTYRAG 2 cut(s) 170, 320
Sse9I AATT 3 cut(s) 403, 428, 588
SseBI AGGCCT 1 cut(s) 168
SspMI CTAG 1 cut(s) 11
StuI AGGCCT 1 cut(s) 168
StyD4I CCNGG 5 cut(s) 123, 417, 504, 711, 837
StyI CCWWGG 2 cut(s) 465, 517
TaaI ACNGT 3 cut(s) 296, 332, 736
TaiI ACGT 4 cut(s) 214, 369, 768, 890
TaqI TCGA 3 cut(s) 315, 321, 704
TasI AATT 3 cut(s) 403, 428, 588
TauI GCSGC 9 cut(s) 474, 503, 866, 869, 1005, 1008, 1011, 1014, 1017
TfiI GAWTC 5 cut(s) 56, 580, 697, 790, 982
Tru1I TTAA 2 cut(s) 171, 663
Tru9I TTAA 2 cut(s) 171, 663
TseFI GTSAC 3 cut(s) 19, 754, 829
TseI GCWGC 3 cut(s) 533, 816, 919
Tsp45I GTSAC 3 cut(s) 19, 754, 829
TspDTI ATGAA 2 cut(s) 214, 710
TspGWI ACGGA 4 cut(s) 649, 690, 786, 848
Vha464I CTTAAG 1 cut(s) 170
VpaK11BI GGWCC 5 cut(s) 36, 229, 258, 421, 835
XapI RAATTY 1 cut(s) 588
XhoI CTCGAG 1 cut(s) 320
XspI CTAG 1 cut(s) 11
Zsp2I ATGCAT 1 cut(s) 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.