RLG00000013401

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007660
Physical Location & Seq
Reverse (-)
31043811 .. 31044520
710 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013401

Sequence Viewer

Length: 453 bp
ATGGGTGTATTGAGAAATTACTCAGACTGGGATGAAGAGGAGAGGGGTGATGGGGATCAAGTTCTCCAAATCCTACTTCACCTTTCTCAGAGGCCTCAAGCTCTTCACTCGCTAATGGCTTCCCCTCATCTCTCCTCCAGTGGCCTCATCTTCATGGTACACGGTTACGGCAACGACATCAGCTGGACTTTTCAGGCCACCATCATCTTCCTCGCCCAGAATGACATCAACGACATAGAATCCCACAGCCGGTCCCACGGCCTCAAAGCCCTCGTCCCAAACGTCGATCTCGTCGTCCAAGACTGCCGCTTCTTCTTCAATTTCGTCAAGCAAAATCCCCAATACGAAACCCTACCGTCCCTCCTCTACGGCGAGTCGATGGGCGGCGCAATATGTCTTCTGATCCACTTCACAGATCCTGAAGCCTTTGATGGTGCACGAGCTTGTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

151

Amino Acids

16.87

Weight (kDa)

5.0

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 47 - 145 2.2e-12 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 307, 384
AclWI GGATC 3 cut(s) 63, 397, 410
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 1 cut(s) 159
AfiI CCNNNNNNNGG 1 cut(s) 249
AgsI TTSAA 1 cut(s) 319
AjuI GAANNNNNNNTTGG 2 cut(s) 60, 92
AluBI AGCT 3 cut(s) 101, 183, 443
AluI AGCT 3 cut(s) 101, 183, 443
Alw21I GWGCWC 1 cut(s) 439
Alw44I GTGCAC 1 cut(s) 435
AlwI GGATC 3 cut(s) 63, 397, 410
AlwNI CAGNNNCTG 1 cut(s) 419
AoxI GGCC 4 cut(s) 92, 142, 195, 259
ApaLI GTGCAC 1 cut(s) 435
ArsI GACNNNNNNTTYG 2 cut(s) 258, 290
AspLEI GCGC 1 cut(s) 389
AspS9I GGNCC 1 cut(s) 252
AsuHPI GGTGA 2 cut(s) 59, 71
AvaII GGWCC 1 cut(s) 252
BaeGI GKGCMC 1 cut(s) 439
BauI CACGAG 1 cut(s) 438
BbsI GAAGAC 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 439
BccI CCATC 4 cut(s) 44, 209, 373, 425
BceAI ACGGC 3 cut(s) 184, 274, 385
BisI GCNGC 2 cut(s) 307, 385
BlsI GCNGC 2 cut(s) 308, 386
Bme18I GGWCC 1 cut(s) 252
BmgT120I GGNCC 1 cut(s) 252
BmiI GGNNCC 1 cut(s) 254
BmrI ACTGGG 1 cut(s) 37
BmuI ACTGGG 1 cut(s) 37
BpiI GAAGAC 1 cut(s) 389
BpmI CTGGAG 1 cut(s) 121
BpuEI CTTGAG 1 cut(s) 81
BsaBI GATNNNNATC 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 256
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 249
Bse118I RCCGGY 1 cut(s) 249
Bse1I ACTGG 2 cut(s) 32, 138
Bse8I GATNNNNATC 1 cut(s) 54
BseDI CCNNGG 1 cut(s) 256
BseGI GGATG 1 cut(s) 37
BseJI GATNNNNATC 1 cut(s) 54
BseLI CCNNNNNNNGG 1 cut(s) 249
BseMII CTCAG 2 cut(s) 36, 101
BseNI ACTGG 2 cut(s) 32, 138
BseRI GAGGAG 3 cut(s) 53, 124, 353
BseSI GKGCMC 1 cut(s) 439
BshFI GGCC 4 cut(s) 94, 144, 197, 261
BsiHKAI GWGCWC 1 cut(s) 439
BsiSI CCGG 1 cut(s) 250
BslFI GGGAC 3 cut(s) 238, 260, 343
BslI CCNNNNNNNGG 1 cut(s) 249
BsmFI GGGAC 3 cut(s) 238, 260, 343
BsnI GGCC 4 cut(s) 94, 144, 197, 261
Bsp1286I GDGCHC 1 cut(s) 439
Bsp143I GATC 4 cut(s) 55, 286, 402, 415
BspACI CCGC 2 cut(s) 307, 384
BspANI GGCC 4 cut(s) 94, 144, 197, 261
BspCNI CTCAG 2 cut(s) 35, 100
BspLI GGNNCC 1 cut(s) 254
BspPI GGATC 3 cut(s) 63, 397, 410
BspQI GCTCTTC 1 cut(s) 108
BsrFI RCCGGY 1 cut(s) 249
BsrI ACTGG 2 cut(s) 32, 138
BssAI RCCGGY 1 cut(s) 249
BssECI CCNNGG 1 cut(s) 256
BssMI GATC 4 cut(s) 55, 286, 402, 415
BssSI CACGAG 1 cut(s) 438
Bst2BI CACGAG 1 cut(s) 438
Bst4CI ACNGT 2 cut(s) 164, 357
Bst6I CTCTTC 2 cut(s) 30, 108
BstDEI CTNAG 2 cut(s) 22, 87
BstDSI CCRYGG 1 cut(s) 256
BstF5I GGATG 1 cut(s) 37
BstHHI GCGC 1 cut(s) 389
BstKTI GATC 4 cut(s) 58, 289, 405, 418
BstMBI GATC 4 cut(s) 55, 286, 402, 415
BstSLI GKGCMC 1 cut(s) 439
BstV2I GAAGAC 1 cut(s) 389
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 4 cut(s) 94, 144, 197, 261
BtgI CCRYGG 1 cut(s) 256
BtsCI GGATG 1 cut(s) 37
BtsIMutI CAGTG 1 cut(s) 145
CaiI CAGNNNCTG 1 cut(s) 419
CfoI GCGC 1 cut(s) 389
Cfr10I RCCGGY 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 252
Csp6I GTAC 1 cut(s) 158
CviAII CATG 1 cut(s) 154
CviQI GTAC 1 cut(s) 158
DdeI CTNAG 2 cut(s) 22, 87
DpnI GATC 4 cut(s) 57, 288, 404, 417
DpnII GATC 4 cut(s) 55, 286, 402, 415
Eam1104I CTCTTC 2 cut(s) 30, 108
EarI CTCTTC 2 cut(s) 30, 108
Eco147I AGGCCT 1 cut(s) 94
Eco47I GGWCC 1 cut(s) 252
Eco57I CTGAAG 1 cut(s) 441
FaeI CATG 1 cut(s) 157
FaiI YATR 3 cut(s) 155, 236, 394
FaqI GGGAC 3 cut(s) 238, 260, 343
FatI CATG 1 cut(s) 153
Fnu4HI GCNGC 2 cut(s) 307, 385
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 2 cut(s) 307, 385
GlaI GCGC 1 cut(s) 388
GluI GCNGC 2 cut(s) 307, 385
GsuI CTGGAG 1 cut(s) 121
HaeIII GGCC 4 cut(s) 94, 144, 197, 261
HapII CCGG 1 cut(s) 250
HhaI GCGC 1 cut(s) 389
Hin1II CATG 1 cut(s) 157
Hin6I GCGC 1 cut(s) 387
HinP1I GCGC 1 cut(s) 387
HinfI GANTC 2 cut(s) 239, 374
HpaII CCGG 1 cut(s) 250
HphI GGTGA 2 cut(s) 59, 71
Hpy166II GTNNAC 2 cut(s) 160, 437
Hpy188I TCNGA 3 cut(s) 25, 90, 402
Hpy188III TCNNGA 1 cut(s) 419
Hpy8I GTNNAC 2 cut(s) 160, 437
Hpy99I CGWCG 2 cut(s) 287, 296
HpyCH4III ACNGT 2 cut(s) 164, 357
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 1 cut(s) 437
HpyF3I CTNAG 2 cut(s) 22, 87
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 1 cut(s) 157
HspAI GCGC 1 cut(s) 387
Kzo9I GATC 4 cut(s) 55, 286, 402, 415
LguI GCTCTTC 1 cut(s) 108
LpnPI CCDG 7 cut(s) 13, 151, 169, 179, 230, 263, 432
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 1 cut(s) 164
MalI GATC 4 cut(s) 57, 288, 404, 417
MboI GATC 4 cut(s) 55, 286, 402, 415
MboII GAAGA 7 cut(s) 47, 95, 142, 199, 304, 307, 389
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 2 cut(s) 16, 319
MlyI GAGTC 1 cut(s) 383
MslI CAYNNNNRTG 1 cut(s) 152
MspA1I CMGCKG 1 cut(s) 183
MspI CCGG 1 cut(s) 250
NdeII GATC 4 cut(s) 55, 286, 402, 415
NlaIII CATG 1 cut(s) 157
NlaIV GGNNCC 1 cut(s) 254
PceI AGGCCT 1 cut(s) 94
PciSI GCTCTTC 1 cut(s) 108
PcsI WCGNNNNNNNCGW 4 cut(s) 279, 288, 291, 445
PfeI GAWTC 1 cut(s) 239
PkrI GCNGC 2 cut(s) 308, 386
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PspN4I GGNNCC 1 cut(s) 254
PspPI GGNCC 1 cut(s) 252
PstNI CAGNNNCTG 1 cut(s) 419
PsuI RGATCY 1 cut(s) 415
PvuII CAGCTG 1 cut(s) 183
RsaI GTAC 1 cut(s) 159
RsaNI GTAC 1 cut(s) 158
RseI CAYNNNNRTG 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 108
SatI GCNGC 2 cut(s) 307, 385
Sau3AI GATC 4 cut(s) 55, 286, 402, 415
Sau96I GGNCC 1 cut(s) 252
SchI GAGTC 1 cut(s) 383
SduI GDGCHC 1 cut(s) 439
SetI ASST 5 cut(s) 84, 103, 185, 285, 445
SinI GGWCC 1 cut(s) 252
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 96
SmoI CTYRAG 1 cut(s) 96
Sse9I AATT 2 cut(s) 16, 319
SseBI AGGCCT 1 cut(s) 94
SsiI CCGC 2 cut(s) 307, 384
StuI AGGCCT 1 cut(s) 94
TaaI ACNGT 2 cut(s) 164, 357
TaiI ACGT 1 cut(s) 285
TaqI TCGA 3 cut(s) 285, 377, 448
TasI AATT 2 cut(s) 16, 319
TauI GCSGC 2 cut(s) 309, 387
TfiI GAWTC 1 cut(s) 239
TscAI CASTG 1 cut(s) 145
TspDTI ATGAA 2 cut(s) 48, 142
TspRI CASTG 1 cut(s) 145
VneI GTGCAC 1 cut(s) 435
VpaK11BI GGWCC 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.