Rroxscaffold_1G00067270

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007660
Physical Location & Seq
Forward (+)
88629115 .. 88629714
600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00067270.1

Sequence Viewer

Length: 513 bp
ATGGCTTCCCTCATCTCTCTCTTCCACGTGGCCTCATCTTCATGGTCCACGGCTACGGCGACGACATCACCGGACTTTTTAGGCCACCGCCATCTTCCTCGCCTAGAACAACTTCGCCGCTTCGCTCTTGACATCGAATCCCACCGCCTTAGAGCCTTCATCCCAAACATCGATCTCGTCGTCCAAGACCGCCTCTTGTTCTTCAATTTCGTCAAGCAAAACCCCCAATATGAAACCCTACCCTCGTCAATGAGGGTGAAGACGGCGAGATTGGAGGGAGATGGCGAGGGAAAGGGACCGGTGAAGTTGCTCGAGCTCGTCAATGAGGGCGAAGACGGCAAGCTTAGAGTGGATGACCTCTTGCTTTGGAACATCGAAATTGGTGGCGAAGAAGATAGGTCTGTTGCATCTTCAAGGTGGGTTTGTGGGTCGGATGATGAAGAAGAGGAAGAGGGCAGGATTTGGATTCAACCCATGATTAGAGCCGGGAGCTCACGGCTTCCCATTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

170

Amino Acids

19.26

Weight (kDa)

5.08

Isoelectric Point (pI)

65.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 88, 118, 145, 190
AcvI CACGTG 1 cut(s) 28
AgeI ACCGGT 1 cut(s) 298
AgsI TTSAA 4 cut(s) 205, 414, 470, 509
AluBI AGCT 3 cut(s) 316, 343, 492
AluI AGCT 3 cut(s) 316, 343, 492
Alw21I GWGCWC 2 cut(s) 318, 494
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 2 cut(s) 30, 82
AsiGI ACCGGT 1 cut(s) 298
Asp700I GAANNNNTTC 1 cut(s) 111
AspS9I GGNCC 2 cut(s) 45, 296
AsuC2I CCSGG 1 cut(s) 487
AsuHPI GGTGA 3 cut(s) 60, 268, 313
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 2 cut(s) 45, 296
BanII GRGCYC 2 cut(s) 318, 494
BbrPI CACGTG 1 cut(s) 28
BbsI GAAGAC 2 cut(s) 266, 339
Bbv12I GWGCWC 2 cut(s) 318, 494
BccI CCATC 2 cut(s) 99, 275
BceAI ACGGC 4 cut(s) 66, 72, 279, 352
BcnI CCSGG 1 cut(s) 487
BfaI CTAG 1 cut(s) 104
BisI GCNGC 1 cut(s) 118
BlsI GCNGC 1 cut(s) 119
Bme1390I CCNGG 1 cut(s) 487
Bme18I GGWCC 2 cut(s) 45, 296
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 2 cut(s) 45, 296
BmiI GGNNCC 1 cut(s) 297
BmrFI CCNGG 1 cut(s) 487
BmsI GCATC 1 cut(s) 416
BpiI GAAGAC 2 cut(s) 266, 339
BpuMI CCSGG 1 cut(s) 487
Bsa29I ATCGAT 1 cut(s) 171
BsaAI YACGTR 1 cut(s) 28
BsaJI CCNNGG 1 cut(s) 48
BsaWI WCCGGW 2 cut(s) 70, 298
Bse118I RCCGGY 1 cut(s) 298
BseCI ATCGAT 1 cut(s) 171
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 3 cut(s) 159, 358, 439
BshFI GGCC 2 cut(s) 32, 84
BshTI ACCGGT 1 cut(s) 298
BshVI ATCGAT 1 cut(s) 171
BsiHKAI GWGCWC 2 cut(s) 318, 494
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 3 cut(s) 71, 299, 486
BslFI GGGAC 1 cut(s) 309
BsmFI GGGAC 1 cut(s) 309
BsnI GGCC 2 cut(s) 32, 84
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 318, 494
Bsp143I GATC 1 cut(s) 172
BspACI CCGC 4 cut(s) 88, 118, 145, 190
BspANI GGCC 2 cut(s) 32, 84
BspDI ATCGAT 1 cut(s) 171
BspLI GGNNCC 1 cut(s) 297
BsrFI RCCGGY 1 cut(s) 298
BssAI RCCGGY 1 cut(s) 298
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 1 cut(s) 172
Bst6I CTCTTC 3 cut(s) 26, 438, 444
BstBAI YACGTR 1 cut(s) 28
BstC8I GCNNGC 1 cut(s) 341
BstDEI CTNAG 2 cut(s) 149, 344
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 3 cut(s) 159, 358, 439
BstKTI GATC 1 cut(s) 175
BstMBI GATC 1 cut(s) 172
BstMWI GCNNNNNNNGC 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 485
BstV2I GAAGAC 2 cut(s) 266, 339
Bsu15I ATCGAT 1 cut(s) 171
BsuRI GGCC 2 cut(s) 32, 84
BsuTUI ATCGAT 1 cut(s) 171
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 3 cut(s) 159, 358, 439
Cac8I GCNNGC 1 cut(s) 341
Cfr10I RCCGGY 1 cut(s) 298
Cfr13I GGNCC 2 cut(s) 45, 296
ClaI ATCGAT 1 cut(s) 171
CspAI ACCGGT 1 cut(s) 298
CviAII CATG 2 cut(s) 42, 475
DdeI CTNAG 2 cut(s) 149, 344
DpnI GATC 1 cut(s) 174
DpnII GATC 1 cut(s) 172
Eam1104I CTCTTC 3 cut(s) 26, 438, 444
EarI CTCTTC 3 cut(s) 26, 438, 444
Ecl136II GAGCTC 2 cut(s) 316, 492
Eco24I GRGCYC 2 cut(s) 318, 494
Eco47I GGWCC 2 cut(s) 45, 296
Eco53kI GAGCTC 2 cut(s) 316, 492
Eco72I CACGTG 1 cut(s) 28
Eco88I CYCGRG 1 cut(s) 311
EcoICRI GAGCTC 2 cut(s) 316, 492
EcoT38I GRGCYC 2 cut(s) 318, 494
FaeI CATG 2 cut(s) 45, 478
FaiI YATR 3 cut(s) 43, 231, 476
FaqI GGGAC 1 cut(s) 309
FatI CATG 2 cut(s) 41, 474
Fnu4HI GCNGC 1 cut(s) 118
FokI GGATG 3 cut(s) 146, 365, 446
FriOI GRGCYC 2 cut(s) 318, 494
Fsp4HI GCNGC 1 cut(s) 118
FspBI CTAG 1 cut(s) 104
GluI GCNGC 1 cut(s) 118
HaeIII GGCC 2 cut(s) 32, 84
HapII CCGG 3 cut(s) 71, 299, 486
Hin1II CATG 2 cut(s) 45, 478
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 2 cut(s) 137, 466
HpaII CCGG 3 cut(s) 71, 299, 486
HphI GGTGA 3 cut(s) 60, 268, 313
Hpy166II GTNNAC 1 cut(s) 48
Hpy188I TCNGA 1 cut(s) 433
Hpy188III TCNNGA 1 cut(s) 128
Hpy8I GTNNAC 1 cut(s) 48
Hpy99I CGWCG 2 cut(s) 64, 182
HpyAV CCTTC 1 cut(s) 166
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 1 cut(s) 407
HpyF10VI GCNNNNNNNGC 1 cut(s) 336
HpyF3I CTNAG 2 cut(s) 149, 344
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 2 cut(s) 45, 478
Kzo9I GATC 1 cut(s) 172
LmnI GCTCC 1 cut(s) 489
LpnPI CCDG 4 cut(s) 84, 312, 442, 499
LweI GCATC 1 cut(s) 416
MaeI CTAG 1 cut(s) 104
MaeII ACGT 1 cut(s) 27
MalI GATC 1 cut(s) 174
MboI GATC 1 cut(s) 172
MhlI GDGCHC 2 cut(s) 318, 494
MluCI AATT 2 cut(s) 205, 378
MmeI TCCRAC 1 cut(s) 411
MroXI GAANNNNTTC 1 cut(s) 111
MslI CAYNNNNRTG 1 cut(s) 40
MspI CCGG 3 cut(s) 71, 299, 486
MspR9I CCNGG 1 cut(s) 487
MwoI GCNNNNNNNGC 1 cut(s) 336
NciI CCSGG 1 cut(s) 487
NdeII GATC 1 cut(s) 172
NlaIII CATG 2 cut(s) 45, 478
NlaIV GGNNCC 1 cut(s) 297
PaeR7I CTCGAG 1 cut(s) 311
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 177
PdmI GAANNNNTTC 1 cut(s) 111
PfeI GAWTC 2 cut(s) 137, 466
PinAI ACCGGT 1 cut(s) 298
PkrI GCNGC 1 cut(s) 119
PmaCI CACGTG 1 cut(s) 28
PmlI CACGTG 1 cut(s) 28
Ppu21I YACGTR 1 cut(s) 28
Psp124BI GAGCTC 2 cut(s) 318, 494
PspCI CACGTG 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 297
PspPI GGNCC 2 cut(s) 45, 296
PspXI VCTCGAGB 1 cut(s) 311
RseI CAYNNNNRTG 1 cut(s) 40
SacI GAGCTC 2 cut(s) 318, 494
SatI GCNGC 1 cut(s) 118
Sau3AI GATC 1 cut(s) 172
Sau96I GGNCC 2 cut(s) 45, 296
ScrFI CCNGG 1 cut(s) 487
SduI GDGCHC 2 cut(s) 318, 494
SetI ASST 7 cut(s) 30, 318, 345, 360, 401, 419, 494
SfaNI GCATC 1 cut(s) 416
Sfr274I CTCGAG 1 cut(s) 311
SinI GGWCC 2 cut(s) 45, 296
SlaI CTCGAG 1 cut(s) 311
SmiMI CAYNNNNRTG 1 cut(s) 40
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 2 cut(s) 205, 378
SsiI CCGC 4 cut(s) 88, 118, 145, 190
SspMI CTAG 1 cut(s) 104
SstI GAGCTC 2 cut(s) 318, 494
StyD4I CCNGG 1 cut(s) 485
TaiI ACGT 1 cut(s) 30
TaqI TCGA 4 cut(s) 135, 171, 312, 375
TasI AATT 2 cut(s) 205, 378
TauI GCSGC 1 cut(s) 120
TfiI GAWTC 2 cut(s) 137, 466
TspDTI ATGAA 4 cut(s) 30, 148, 246, 453
VpaK11BI GGWCC 2 cut(s) 45, 296
XhoI CTCGAG 1 cut(s) 311
XmnI GAANNNNTTC 1 cut(s) 111
XspI CTAG 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.