Rh5DG052500

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007660
Physical Location & Seq
Reverse (-)
4475607 .. 4476386
780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG052500.1

Sequence Viewer

Length: 780 bp
ATGGTCCACGGCTACGGCAACGACATCAGCTGGACTTTTCAGGCCACCGCCATCTTCCTCGCCCAGAACGGCTTCGCTTGCTTCGCTCTCGACCTCGAATCCCACGGCCGCTCCCACGGCCTCCGAGCCTTCGTCCCGAACGTCGATCTCGTCGTCCAAGACTGCCGCTTTTTCTTCAGTTTCGTCAAGCAAAACCCCCAATACGAAACCCTACCGTCCTTCCTCTACGGCGAGTCCATGGGCGGCGCGATCTGTCTTCTGATCCACTTCGCCGATCCCGAAGCCTTCCACGGCGCGATTCTGGTGGCGCCGATGTGCAAAATCGCCGACGCCGTCAAGCCGAAGTGGCCGATCCCTCAGATCCTGACGTTCGTCGCCAAGTTTCTCCCCACACTGGCGGTTGTGCCCACCGGGGACTTGATGGTCAAATCGGTCAAGGTCGAGGACAAAAAGGTAATTGCGGACATGAATCCCTCTAGGTACAGAGGGAGGCCCAGATTGGGTACAGTTCTGGAGCTGCTGAGAGTTACAGACCATGTGAGCCGGAGACTCTGTGATGTCAGCCTGCCGTTTCTTGTGCTACACGGCAGCGCCGACGTGGTGACTGATCCTAATGTGAGCAGAGCTTTGTATGAAGAAGCAAAGAGTGAGGACAAGACGATCAAGATTTACGACGGGATGATGCATTCCTTGTTGTTTGGGGAGACTGATGAGAATGTCGAAATTGTTCGCGGCGACATTCTGGCTTGGTTGGATCAGAGGTGCGGAGCAAGATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

259

Amino Acids

28.91

Weight (kDa)

6.06

Isoelectric Point (pI)

28.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_6 PF12697 1 - 233 1.2e-11 Alpha/beta hydrolase family
Hydrolase_4 PF12146 2 - 235 2.8e-54 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 422
AccB1I GGYRCC 1 cut(s) 307
AccBSI CCGCTC 1 cut(s) 111
AccII CGCG 3 cut(s) 248, 296, 732
AciI CCGC 8 cut(s) 48, 109, 166, 243, 398, 461, 732, 765
AclWI GGATC 6 cut(s) 256, 269, 346, 355, 602, 762
AcoI YGGCCR 2 cut(s) 106, 347
AcuI CTGAAG 1 cut(s) 160
AcyI GRCGYC 2 cut(s) 308, 330
AfaI GTAC 2 cut(s) 482, 505
AfiI CCNNNNNNNGG 2 cut(s) 394, 500
AjiI CACGTC 1 cut(s) 598
AluBI AGCT 3 cut(s) 30, 517, 626
AluI AGCT 3 cut(s) 30, 517, 626
Alw26I GTCTC 2 cut(s) 541, 698
AlwI GGATC 6 cut(s) 256, 269, 346, 355, 602, 762
AlwNI CAGNNNCTG 1 cut(s) 364
AoxI GGCC 5 cut(s) 42, 106, 118, 347, 491
ApeKI GCWGC 2 cut(s) 517, 588
Asp700I GAANNNNTTC 2 cut(s) 71, 726
AspLEI GCGC 4 cut(s) 248, 296, 310, 593
AspS9I GGNCC 2 cut(s) 4, 492
AsuC2I CCSGG 1 cut(s) 412
AsuHPI GGTGA 1 cut(s) 613
AvaII GGWCC 1 cut(s) 4
BaeGI GKGCMC 1 cut(s) 408
BanI GGYRCC 1 cut(s) 307
BbsI GAAGAC 1 cut(s) 248
BbvI GCAGC 2 cut(s) 504, 600
BccI CCATC 2 cut(s) 59, 415
BcnI CCSGG 1 cut(s) 412
BcoDI GTCTC 2 cut(s) 541, 698
BfaI CTAG 1 cut(s) 477
BfoI RGCGCY 2 cut(s) 311, 594
BglI GCCNNNNNGGC 1 cut(s) 346
BisI GCNGC 6 cut(s) 109, 166, 244, 518, 589, 733
BlsI GCNGC 6 cut(s) 110, 167, 245, 519, 590, 734
Bme1390I CCNGG 1 cut(s) 412
Bme18I GGWCC 1 cut(s) 4
BmgBI CACGTC 1 cut(s) 598
BmgT120I GGNCC 2 cut(s) 4, 492
BmiI GGNNCC 1 cut(s) 309
BmrFI CCNGG 1 cut(s) 412
BmsI GCATC 1 cut(s) 672
BoxI GACNNNNGTC 1 cut(s) 371
BpiI GAAGAC 1 cut(s) 248
BpmI CTGGAG 1 cut(s) 533
BpuMI CCSGG 1 cut(s) 412
BsaHI GRCGYC 2 cut(s) 308, 330
BsaJI CCNNGG 6 cut(s) 7, 103, 115, 237, 289, 411
BsaXI ACNNNNNCTCC 4 cut(s) 95, 125, 538, 568
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 500
Bse1I ACTGG 1 cut(s) 399
BseDI CCNNGG 6 cut(s) 7, 103, 115, 237, 289, 411
BseGI GGATG 1 cut(s) 684
BseLI CCNNNNNNNGG 2 cut(s) 394, 500
BseMII CTCAG 2 cut(s) 371, 512
BseNI ACTGG 1 cut(s) 399
BseSI GKGCMC 1 cut(s) 408
BseX3I CGGCCG 1 cut(s) 106
BseXI GCAGC 2 cut(s) 504, 600
Bsh1236I CGCG 3 cut(s) 248, 296, 732
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 5 cut(s) 44, 108, 120, 349, 493
BshNI GGYRCC 1 cut(s) 307
BsiEI CGRYCG 1 cut(s) 109
BsiSI CCGG 2 cut(s) 411, 544
BslFI GGGAC 2 cut(s) 119, 428
BslI CCNNNNNNNGG 2 cut(s) 394, 500
BsmAI GTCTC 2 cut(s) 541, 698
BsmFI GGGAC 2 cut(s) 119, 428
BsmI GAATGC 1 cut(s) 685
BsnI GGCC 5 cut(s) 44, 108, 120, 349, 493
Bsp1286I GDGCHC 1 cut(s) 408
Bsp143I GATC 9 cut(s) 145, 249, 261, 274, 351, 360, 607, 660, 754
Bsp19I CCATGG 1 cut(s) 237
BspACI CCGC 8 cut(s) 48, 109, 166, 243, 398, 461, 732, 765
BspANI GGCC 5 cut(s) 44, 108, 120, 349, 493
BspCNI CTCAG 2 cut(s) 370, 513
BspFNI CGCG 3 cut(s) 248, 296, 732
BspLI GGNNCC 1 cut(s) 309
BspPI GGATC 6 cut(s) 256, 269, 346, 355, 602, 762
BspT107I GGYRCC 1 cut(s) 307
BsrBI CCGCTC 1 cut(s) 111
BsrI ACTGG 1 cut(s) 399
BssECI CCNNGG 6 cut(s) 7, 103, 115, 237, 289, 411
BssMI GATC 9 cut(s) 145, 249, 261, 274, 351, 360, 607, 660, 754
BssNI GRCGYC 2 cut(s) 308, 330
BssT1I CCWWGG 1 cut(s) 237
Bst4CI ACNGT 2 cut(s) 216, 508
BstACI GRCGYC 2 cut(s) 308, 330
BstC8I GCNNGC 2 cut(s) 79, 566
BstDEI CTNAG 2 cut(s) 357, 521
BstDSI CCRYGG 5 cut(s) 7, 103, 115, 237, 289
BstF5I GGATG 1 cut(s) 684
BstFNI CGCG 3 cut(s) 248, 296, 732
BstH2I RGCGCY 2 cut(s) 311, 594
BstHHI GCGC 4 cut(s) 248, 296, 310, 593
BstKTI GATC 9 cut(s) 148, 252, 264, 277, 354, 363, 610, 663, 757
BstMAI GTCTC 2 cut(s) 541, 698
BstMBI GATC 9 cut(s) 145, 249, 261, 274, 351, 360, 607, 660, 754
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 4 cut(s) 78, 83, 117, 346
BstPAI GACNNNNGTC 1 cut(s) 371
BstSCI CCNGG 1 cut(s) 410
BstSLI GKGCMC 1 cut(s) 408
BstUI CGCG 3 cut(s) 248, 296, 732
BstV1I GCAGC 2 cut(s) 504, 600
BstV2I GAAGAC 1 cut(s) 248
BstX2I RGATCY 1 cut(s) 360
BstYI RGATCY 1 cut(s) 360
BstZI CGGCCG 1 cut(s) 106
BsuRI GGCC 5 cut(s) 44, 108, 120, 349, 493
BtgI CCRYGG 5 cut(s) 7, 103, 115, 237, 289
BtrI CACGTC 1 cut(s) 598
BtsCI GGATG 1 cut(s) 684
BtsIMutI CAGTG 1 cut(s) 392
Cac8I GCNNGC 2 cut(s) 79, 566
CaiI CAGNNNCTG 1 cut(s) 364
CfoI GCGC 4 cut(s) 248, 296, 310, 593
Cfr13I GGNCC 2 cut(s) 4, 492
CseI GACGC 1 cut(s) 338
Csp6I GTAC 2 cut(s) 481, 504
CviAII CATG 3 cut(s) 238, 466, 536
CviQI GTAC 2 cut(s) 481, 504
DdeI CTNAG 2 cut(s) 357, 521
DinI GGCGCC 1 cut(s) 309
DpnI GATC 9 cut(s) 147, 251, 263, 276, 353, 362, 609, 662, 756
DpnII GATC 9 cut(s) 145, 249, 261, 274, 351, 360, 607, 660, 754
DrdI GACNNNNNNGTC 1 cut(s) 422
DseDI GACNNNNNNGTC 1 cut(s) 422
EaeI YGGCCR 2 cut(s) 106, 347
EagI CGGCCG 1 cut(s) 106
EclXI CGGCCG 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 4
Eco52I CGGCCG 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 160
EcoT14I CCWWGG 1 cut(s) 237
EcoT22I ATGCAT 1 cut(s) 687
EgeI GGCGCC 1 cut(s) 309
EheI GGCGCC 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 237
FaeI CATG 3 cut(s) 241, 469, 539
FaiI YATR 4 cut(s) 239, 467, 537, 633
FaqI GGGAC 2 cut(s) 119, 428
FatI CATG 3 cut(s) 237, 465, 535
Fnu4HI GCNGC 6 cut(s) 109, 166, 244, 518, 589, 733
FokI GGATG 1 cut(s) 691
Fsp4HI GCNGC 6 cut(s) 109, 166, 244, 518, 589, 733
FspBI CTAG 1 cut(s) 477
GlaI GCGC 4 cut(s) 247, 295, 309, 592
GluI GCNGC 6 cut(s) 109, 166, 244, 518, 589, 733
GsuI CTGGAG 1 cut(s) 533
HaeII RGCGCY 2 cut(s) 311, 594
HaeIII GGCC 5 cut(s) 44, 108, 120, 349, 493
HapII CCGG 2 cut(s) 411, 544
HgaI GACGC 1 cut(s) 338
HhaI GCGC 4 cut(s) 248, 296, 310, 593
Hin1I GRCGYC 2 cut(s) 308, 330
Hin1II CATG 3 cut(s) 241, 469, 539
Hin6I GCGC 4 cut(s) 246, 294, 308, 591
HinP1I GCGC 4 cut(s) 246, 294, 308, 591
HinfI GANTC 5 cut(s) 98, 233, 298, 469, 549
HpaII CCGG 2 cut(s) 411, 544
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 4 cut(s) 125, 261, 360, 759
Hpy188III TCNNGA 6 cut(s) 89, 136, 278, 364, 512, 664
Hpy8I GTNNAC 1 cut(s) 7
Hpy99I CGWCG 6 cut(s) 146, 155, 332, 377, 599, 677
HpyAV CCTTC 3 cut(s) 139, 229, 295
HpyCH4III ACNGT 2 cut(s) 216, 508
HpyCH4IV ACGT 3 cut(s) 141, 368, 597
HpyCH4V TGCA 2 cut(s) 318, 685
HpyF10VI GCNNNNNNNGC 4 cut(s) 78, 83, 117, 346
HpyF3I CTNAG 2 cut(s) 357, 521
HpySE526I ACGT 3 cut(s) 141, 368, 597
Hsp92I GRCGYC 2 cut(s) 308, 330
Hsp92II CATG 3 cut(s) 241, 469, 539
HspAI GCGC 4 cut(s) 246, 294, 308, 591
KasI GGCGCC 1 cut(s) 307
Kzo9I GATC 9 cut(s) 145, 249, 261, 274, 351, 360, 607, 660, 754
LmnI GCTCC 3 cut(s) 116, 514, 767
Lsp1109I GCAGC 2 cut(s) 504, 600
LweI GCATC 1 cut(s) 672
MaeI CTAG 1 cut(s) 477
MaeII ACGT 3 cut(s) 141, 368, 597
MaeIII GTNAC 2 cut(s) 526, 601
MalI GATC 9 cut(s) 147, 251, 263, 276, 353, 362, 609, 662, 756
MbiI CCGCTC 1 cut(s) 111
MboI GATC 9 cut(s) 145, 249, 261, 274, 351, 360, 607, 660, 754
MboII GAAGA 4 cut(s) 46, 166, 248, 647
MflI RGATCY 1 cut(s) 360
MhlI GDGCHC 1 cut(s) 408
MluCI AATT 2 cut(s) 456, 723
Mly113I GGCGCC 1 cut(s) 308
MlyI GAGTC 2 cut(s) 242, 543
MmeI TCCRAC 1 cut(s) 732
Mph1103I ATGCAT 1 cut(s) 687
MroXI GAANNNNTTC 2 cut(s) 71, 726
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 2 cut(s) 411, 544
MspR9I CCNGG 1 cut(s) 412
Mva1269I GAATGC 1 cut(s) 685
MvnI CGCG 3 cut(s) 248, 296, 732
MwoI GCNNNNNNNGC 4 cut(s) 78, 83, 117, 346
NarI GGCGCC 1 cut(s) 308
NciI CCSGG 1 cut(s) 412
NcoI CCATGG 1 cut(s) 237
NdeII GATC 9 cut(s) 145, 249, 261, 274, 351, 360, 607, 660, 754
NlaIII CATG 3 cut(s) 241, 469, 539
NlaIV GGNNCC 1 cut(s) 309
NmuCI GTSAC 1 cut(s) 601
NsiI ATGCAT 1 cut(s) 687
PcsI WCGNNNNNNNCGW 6 cut(s) 138, 147, 150, 276, 330, 591
PctI GAATGC 1 cut(s) 685
PdmI GAANNNNTTC 2 cut(s) 71, 726
PfeI GAWTC 3 cut(s) 98, 298, 469
PflFI GACNNNGTC 1 cut(s) 332
PkrI GCNGC 6 cut(s) 110, 167, 245, 519, 590, 734
PleI GAGTC 2 cut(s) 241, 543
PluTI GGCGCC 1 cut(s) 311
PpsI GAGTC 2 cut(s) 241, 543
PshAI GACNNNNGTC 1 cut(s) 371
PspN4I GGNNCC 1 cut(s) 309
PspPI GGNCC 2 cut(s) 4, 492
PstNI CAGNNNCTG 1 cut(s) 364
PsuI RGATCY 1 cut(s) 360
PsyI GACNNNGTC 1 cut(s) 332
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 2 cut(s) 482, 505
RsaNI GTAC 2 cut(s) 481, 504
SatI GCNGC 6 cut(s) 109, 166, 244, 518, 589, 733
Sau3AI GATC 9 cut(s) 145, 249, 261, 274, 351, 360, 607, 660, 754
Sau96I GGNCC 2 cut(s) 4, 492
SchI GAGTC 2 cut(s) 242, 543
ScrFI CCNGG 1 cut(s) 412
SduI GDGCHC 1 cut(s) 408
SfaNI GCATC 1 cut(s) 672
SfoI GGCGCC 1 cut(s) 309
SinI GGWCC 1 cut(s) 4
Sse9I AATT 2 cut(s) 456, 723
SsiI CCGC 8 cut(s) 48, 109, 166, 243, 398, 461, 732, 765
SspDI GGCGCC 1 cut(s) 307
SspMI CTAG 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 410
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 2 cut(s) 216, 508
TaiI ACGT 3 cut(s) 144, 371, 600
TaqI TCGA 5 cut(s) 90, 96, 144, 441, 720
TaqII GACCGA 1 cut(s) 421
TasI AATT 2 cut(s) 456, 723
TauI GCSGC 4 cut(s) 111, 168, 246, 735
TfiI GAWTC 3 cut(s) 98, 298, 469
TscAI CASTG 1 cut(s) 399
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 2 cut(s) 517, 588
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 2 cut(s) 482, 648
TspRI CASTG 1 cut(s) 399
Tth111I GACNNNGTC 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 4
XmnI GAANNNNTTC 2 cut(s) 71, 726
XspI CTAG 1 cut(s) 477
Zsp2I ATGCAT 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.