Rw5G005430

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007660
Physical Location & Seq
Forward (+)
5686885 .. 5687835
951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G005430.1

Sequence Viewer

Length: 951 bp
ATGAAGGAGGAGCAAAAATGGCCTCACTACTGGGGCACCACACCGGAGCAAGACTACTACGATCAGCAGGGGATCAAATCGTCCAAATCCTACTTCACCTCTCCCAGAGGCCTCAGGCTCTTCACCCGCCAATGGCTTCCCATCGTCTCTCCTCCACGTGGCCTCATCTTCATGGTCCACGGCTACGGCAACGACATCAGCTGGACTTTTCAGGCCACCGCCATCTTCCTCGCCCAGAACGGCTTCGCCTGCTTCGCTCTCGACCTCGAATCCCACGGCCGCTCCCACGGCCTCCGAGCCTTCGTCCCCAACGTCGATCTCGTCGTCCAAGACTGCCGCTTCTTCTTCAGTTTCGTCAAGCAAAACCCCCAATACGAAACCCTACCGTCCTTCCTCTACGGCGAGTCGATGGGCGGCGCGATCTGTCTTCTGATCCACTTCGCCGATCCCGAAGCCTTCCACGGCGCGATTCTGGTGGCGCCGATGTGCAAAATCGCCGACGCCGTCAAGCCCAAGTGGCCGATCCCTCAGATCCTGACGTTCGTCGCCAAGTTTCTCCCCACATTGGCGGTTGTGCCCACCGGGGACTTGATGGTCAAATCGGTCAAGGTCGAGGACAAAAAGGTAATTGCGGACATGAATCCCTCAAGGTACAGAGGGAGGCCCAGATTGGGTACAGTTCTGGAGCTACTGAGAGTTACAGACCATGTGAGCCGGAGACTCTGTGATGTCAGCCTGCCGTTTCTTGTGCTACACGGCAGCGCCGACGTGGTGACTGATCCTAATGTGAGCAGGGCTTTGTATGAAGAAGCAAAGAGTGAGGACAAGACGATCAAGATTTACGACGGGATGATGCATTCCTTGTTGTTTGGGGAGACTGATGAGAATGTCGAAATTGTTCGCGCCGACATTCTGGCTTGGTTGGATCAGAGGTGCAGAGCAAGAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

316

Amino Acids

35.83

Weight (kDa)

7.11

Isoelectric Point (pI)

36.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 51 - 292 9.2e-57 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 56 - 290 6e-12 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 593
AccB1I GGYRCC 2 cut(s) 35, 478
AccBSI CCGCTC 1 cut(s) 282
AccII CGCG 3 cut(s) 419, 467, 903
AciI CCGC 7 cut(s) 127, 219, 280, 337, 414, 569, 632
AclWI GGATC 7 cut(s) 80, 427, 440, 517, 526, 773, 933
AcoI YGGCCR 2 cut(s) 277, 518
AcuI CTGAAG 1 cut(s) 331
AcvI CACGTG 1 cut(s) 158
AcyI GRCGYC 2 cut(s) 479, 501
AfaI GTAC 2 cut(s) 653, 676
AfiI CCNNNNNNNGG 4 cut(s) 132, 158, 565, 671
AjiI CACGTC 1 cut(s) 769
AjuI GAANNNNNNNTTGG 2 cut(s) 77, 109
AluBI AGCT 2 cut(s) 201, 688
AluI AGCT 2 cut(s) 201, 688
Alw26I GTCTC 3 cut(s) 151, 712, 869
AlwI GGATC 7 cut(s) 80, 427, 440, 517, 526, 773, 933
AlwNI CAGNNNCTG 1 cut(s) 535
AoxI GGCC 8 cut(s) 20, 109, 160, 213, 277, 289, 518, 662
ApeKI GCWGC 1 cut(s) 759
Asp700I GAANNNNTTC 2 cut(s) 242, 897
AspLEI GCGC 5 cut(s) 419, 467, 481, 764, 905
AspS9I GGNCC 2 cut(s) 175, 663
AsuC2I CCSGG 1 cut(s) 583
AsuHPI GGTGA 3 cut(s) 88, 115, 784
AvaII GGWCC 1 cut(s) 175
AxyI CCTNAGG 1 cut(s) 113
BaeGI GKGCMC 2 cut(s) 38, 579
BanI GGYRCC 2 cut(s) 35, 478
BbrPI CACGTG 1 cut(s) 158
BbsI GAAGAC 1 cut(s) 419
BbvI GCAGC 1 cut(s) 771
BccI CCATC 4 cut(s) 149, 230, 403, 586
BcnI CCSGG 1 cut(s) 583
BcoDI GTCTC 3 cut(s) 151, 712, 869
BfoI RGCGCY 2 cut(s) 482, 765
BglI GCCNNNNNGGC 1 cut(s) 517
BisI GCNGC 4 cut(s) 280, 337, 415, 760
BlsI GCNGC 4 cut(s) 281, 338, 416, 761
Bme1390I CCNGG 1 cut(s) 583
Bme18I GGWCC 1 cut(s) 175
BmgBI CACGTC 1 cut(s) 769
BmgT120I GGNCC 2 cut(s) 175, 663
BmiI GGNNCC 2 cut(s) 37, 480
BmrFI CCNGG 1 cut(s) 583
BmrI ACTGGG 1 cut(s) 40
BmsI GCATC 1 cut(s) 843
BmuI ACTGGG 1 cut(s) 40
BoxI GACNNNNGTC 1 cut(s) 542
BpiI GAAGAC 1 cut(s) 419
BpmI CTGGAG 1 cut(s) 704
BpuEI CTTGAG 1 cut(s) 631
BpuMI CCSGG 1 cut(s) 583
BsaAI YACGTR 1 cut(s) 158
BsaHI GRCGYC 2 cut(s) 479, 501
BsaJI CCNNGG 5 cut(s) 178, 274, 286, 460, 582
BsaWI WCCGGW 1 cut(s) 43
BsaXI ACNNNNNCTCC 4 cut(s) 266, 296, 709, 739
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 158, 565, 671
Bse1I ACTGG 1 cut(s) 35
Bse21I CCTNAGG 1 cut(s) 113
BseDI CCNNGG 5 cut(s) 178, 274, 286, 460, 582
BseGI GGATG 1 cut(s) 855
BseLI CCNNNNNNNGG 4 cut(s) 132, 158, 565, 671
BseMII CTCAG 3 cut(s) 127, 542, 683
BseNI ACTGG 1 cut(s) 35
BseRI GAGGAG 2 cut(s) 23, 141
BseSI GKGCMC 2 cut(s) 38, 579
BseX3I CGGCCG 1 cut(s) 277
BseXI GCAGC 1 cut(s) 771
Bsh1236I CGCG 3 cut(s) 419, 467, 903
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 8 cut(s) 22, 111, 162, 215, 279, 291, 520, 664
BshNI GGYRCC 2 cut(s) 35, 478
BsiEI CGRYCG 1 cut(s) 280
BsiSI CCGG 3 cut(s) 44, 582, 715
BslFI GGGAC 2 cut(s) 290, 599
BslI CCNNNNNNNGG 4 cut(s) 132, 158, 565, 671
BsmAI GTCTC 3 cut(s) 151, 712, 869
BsmBI CGTCTC 1 cut(s) 151
BsmFI GGGAC 2 cut(s) 290, 599
BsmI GAATGC 1 cut(s) 856
BsnI GGCC 8 cut(s) 22, 111, 162, 215, 279, 291, 520, 664
Bsp1286I GDGCHC 2 cut(s) 38, 579
BspACI CCGC 7 cut(s) 127, 219, 280, 337, 414, 569, 632
BspANI GGCC 8 cut(s) 22, 111, 162, 215, 279, 291, 520, 664
BspCNI CTCAG 3 cut(s) 126, 541, 684
BspFNI CGCG 3 cut(s) 419, 467, 903
BspLI GGNNCC 2 cut(s) 37, 480
BspPI GGATC 7 cut(s) 80, 427, 440, 517, 526, 773, 933
BspQI GCTCTTC 1 cut(s) 125
BspT107I GGYRCC 2 cut(s) 35, 478
BsrBI CCGCTC 1 cut(s) 282
BsrI ACTGG 1 cut(s) 35
BssECI CCNNGG 5 cut(s) 178, 274, 286, 460, 582
BssNI GRCGYC 2 cut(s) 479, 501
Bst4CI ACNGT 2 cut(s) 387, 679
Bst6I CTCTTC 1 cut(s) 125
BstACI GRCGYC 2 cut(s) 479, 501
BstBAI YACGTR 1 cut(s) 158
BstC8I GCNNGC 2 cut(s) 250, 737
BstDEI CTNAG 3 cut(s) 113, 528, 692
BstDSI CCRYGG 4 cut(s) 178, 274, 286, 460
BstF5I GGATG 1 cut(s) 855
BstFNI CGCG 3 cut(s) 419, 467, 903
BstH2I RGCGCY 2 cut(s) 482, 765
BstHHI GCGC 5 cut(s) 419, 467, 481, 764, 905
BstMAI GTCTC 3 cut(s) 151, 712, 869
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 5 cut(s) 19, 249, 254, 288, 517
BstPAI GACNNNNGTC 1 cut(s) 542
BstSCI CCNGG 1 cut(s) 581
BstSLI GKGCMC 2 cut(s) 38, 579
BstUI CGCG 3 cut(s) 419, 467, 903
BstV1I GCAGC 1 cut(s) 771
BstV2I GAAGAC 1 cut(s) 419
BstX2I RGATCY 1 cut(s) 531
BstYI RGATCY 1 cut(s) 531
BstZI CGGCCG 1 cut(s) 277
Bsu36I CCTNAGG 1 cut(s) 113
BsuRI GGCC 8 cut(s) 22, 111, 162, 215, 279, 291, 520, 664
BtgI CCRYGG 4 cut(s) 178, 274, 286, 460
BtrI CACGTC 1 cut(s) 769
BtsCI GGATG 1 cut(s) 855
Cac8I GCNNGC 2 cut(s) 250, 737
CaiI CAGNNNCTG 1 cut(s) 535
CfoI GCGC 5 cut(s) 419, 467, 481, 764, 905
Cfr13I GGNCC 2 cut(s) 175, 663
CseI GACGC 1 cut(s) 509
Csp6I GTAC 2 cut(s) 652, 675
CviAII CATG 3 cut(s) 172, 637, 707
CviQI GTAC 2 cut(s) 652, 675
DdeI CTNAG 3 cut(s) 113, 528, 692
DinI GGCGCC 1 cut(s) 480
DrdI GACNNNNNNGTC 1 cut(s) 593
DseDI GACNNNNNNGTC 1 cut(s) 593
EaeI YGGCCR 2 cut(s) 277, 518
EagI CGGCCG 1 cut(s) 277
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
EclXI CGGCCG 1 cut(s) 277
Eco147I AGGCCT 1 cut(s) 111
Eco47I GGWCC 1 cut(s) 175
Eco52I CGGCCG 1 cut(s) 277
Eco57I CTGAAG 1 cut(s) 331
Eco72I CACGTG 1 cut(s) 158
Eco81I CCTNAGG 1 cut(s) 113
EcoT22I ATGCAT 1 cut(s) 858
EgeI GGCGCC 1 cut(s) 480
EheI GGCGCC 1 cut(s) 480
Esp3I CGTCTC 1 cut(s) 151
FaeI CATG 3 cut(s) 175, 640, 710
FaiI YATR 4 cut(s) 173, 638, 708, 804
FaqI GGGAC 2 cut(s) 290, 599
FatI CATG 3 cut(s) 171, 636, 706
FauI CCCGC 1 cut(s) 134
Fnu4HI GCNGC 4 cut(s) 280, 337, 415, 760
FokI GGATG 1 cut(s) 862
Fsp4HI GCNGC 4 cut(s) 280, 337, 415, 760
GlaI GCGC 5 cut(s) 418, 466, 480, 763, 904
GluI GCNGC 4 cut(s) 280, 337, 415, 760
GsuI CTGGAG 1 cut(s) 704
HaeII RGCGCY 2 cut(s) 482, 765
HaeIII GGCC 8 cut(s) 22, 111, 162, 215, 279, 291, 520, 664
HapII CCGG 3 cut(s) 44, 582, 715
HgaI GACGC 1 cut(s) 509
HhaI GCGC 5 cut(s) 419, 467, 481, 764, 905
Hin1I GRCGYC 2 cut(s) 479, 501
Hin1II CATG 3 cut(s) 175, 640, 710
Hin6I GCGC 5 cut(s) 417, 465, 479, 762, 903
HinP1I GCGC 5 cut(s) 417, 465, 479, 762, 903
HinfI GANTC 5 cut(s) 269, 404, 469, 640, 720
HpaII CCGG 3 cut(s) 44, 582, 715
HphI GGTGA 3 cut(s) 88, 115, 784
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 4 cut(s) 296, 432, 531, 930
Hpy188III TCNNGA 5 cut(s) 260, 449, 535, 683, 835
Hpy8I GTNNAC 1 cut(s) 178
Hpy99I CGWCG 6 cut(s) 317, 326, 503, 548, 770, 848
HpyAV CCTTC 3 cut(s) 310, 400, 466
HpyCH4III ACNGT 2 cut(s) 387, 679
HpyCH4IV ACGT 4 cut(s) 157, 312, 539, 768
HpyCH4V TGCA 3 cut(s) 489, 856, 936
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 249, 254, 288, 517
HpyF3I CTNAG 3 cut(s) 113, 528, 692
HpySE526I ACGT 4 cut(s) 157, 312, 539, 768
Hsp92I GRCGYC 2 cut(s) 479, 501
Hsp92II CATG 3 cut(s) 175, 640, 710
HspAI GCGC 5 cut(s) 417, 465, 479, 762, 903
KasI GGCGCC 1 cut(s) 478
LguI GCTCTTC 1 cut(s) 125
LmnI GCTCC 4 cut(s) 10, 46, 287, 685
Lsp1109I GCAGC 1 cut(s) 771
LweI GCATC 1 cut(s) 843
MaeII ACGT 4 cut(s) 157, 312, 539, 768
MaeIII GTNAC 2 cut(s) 697, 772
MbiI CCGCTC 1 cut(s) 282
MboII GAAGA 7 cut(s) 112, 160, 217, 334, 337, 419, 818
MflI RGATCY 1 cut(s) 531
MhlI GDGCHC 2 cut(s) 38, 579
MluCI AATT 3 cut(s) 627, 894, 946
Mly113I GGCGCC 1 cut(s) 479
MlyI GAGTC 2 cut(s) 413, 714
MmeI TCCRAC 1 cut(s) 903
Mph1103I ATGCAT 1 cut(s) 858
MroXI GAANNNNTTC 2 cut(s) 242, 897
MslI CAYNNNNRTG 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 201
MspI CCGG 3 cut(s) 44, 582, 715
MspR9I CCNGG 1 cut(s) 583
Mva1269I GAATGC 1 cut(s) 856
MvnI CGCG 3 cut(s) 419, 467, 903
MwoI GCNNNNNNNGC 5 cut(s) 19, 249, 254, 288, 517
NarI GGCGCC 1 cut(s) 479
NciI CCSGG 1 cut(s) 583
NlaIII CATG 3 cut(s) 175, 640, 710
NlaIV GGNNCC 2 cut(s) 37, 480
NmuCI GTSAC 1 cut(s) 772
NsiI ATGCAT 1 cut(s) 858
PceI AGGCCT 1 cut(s) 111
PciSI GCTCTTC 1 cut(s) 125
PcsI WCGNNNNNNNCGW 6 cut(s) 309, 318, 321, 447, 501, 762
PctI GAATGC 1 cut(s) 856
PdmI GAANNNNTTC 2 cut(s) 242, 897
PfeI GAWTC 3 cut(s) 269, 469, 640
PflFI GACNNNGTC 1 cut(s) 503
PkrI GCNGC 4 cut(s) 281, 338, 416, 761
PleI GAGTC 2 cut(s) 412, 714
PluTI GGCGCC 1 cut(s) 482
PmaCI CACGTG 1 cut(s) 158
PmlI CACGTG 1 cut(s) 158
PpsI GAGTC 2 cut(s) 412, 714
Ppu21I YACGTR 1 cut(s) 158
PshAI GACNNNNGTC 1 cut(s) 542
PspCI CACGTG 1 cut(s) 158
PspN4I GGNNCC 2 cut(s) 37, 480
PspPI GGNCC 2 cut(s) 175, 663
PstNI CAGNNNCTG 1 cut(s) 535
PsuI RGATCY 1 cut(s) 531
PsyI GACNNNGTC 1 cut(s) 503
PvuII CAGCTG 1 cut(s) 201
RsaI GTAC 2 cut(s) 653, 676
RsaNI GTAC 2 cut(s) 652, 675
RseI CAYNNNNRTG 1 cut(s) 170
SapI GCTCTTC 1 cut(s) 125
SatI GCNGC 4 cut(s) 280, 337, 415, 760
Sau96I GGNCC 2 cut(s) 175, 663
SchI GAGTC 2 cut(s) 413, 714
ScrFI CCNGG 1 cut(s) 583
SduI GDGCHC 2 cut(s) 38, 579
SfaNI GCATC 1 cut(s) 843
SfoI GGCGCC 1 cut(s) 480
SinI GGWCC 1 cut(s) 175
SmiMI CAYNNNNRTG 1 cut(s) 170
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
Sse9I AATT 3 cut(s) 627, 894, 946
SseBI AGGCCT 1 cut(s) 111
SsiI CCGC 7 cut(s) 127, 219, 280, 337, 414, 569, 632
SspDI GGCGCC 1 cut(s) 478
StuI AGGCCT 1 cut(s) 111
StyD4I CCNGG 1 cut(s) 581
TaaI ACNGT 2 cut(s) 387, 679
TaiI ACGT 4 cut(s) 160, 315, 542, 771
TaqI TCGA 6 cut(s) 261, 267, 315, 407, 612, 891
TaqII GACCGA 1 cut(s) 592
TasI AATT 3 cut(s) 627, 894, 946
TauI GCSGC 3 cut(s) 282, 339, 417
TfiI GAWTC 3 cut(s) 269, 469, 640
TseFI GTSAC 1 cut(s) 772
TseI GCWGC 1 cut(s) 759
Tsp45I GTSAC 1 cut(s) 772
TspDTI ATGAA 4 cut(s) 17, 160, 653, 819
Tth111I GACNNNGTC 1 cut(s) 503
VpaK11BI GGWCC 1 cut(s) 175
XmnI GAANNNNTTC 2 cut(s) 242, 897
Zsp2I ATGCAT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.