MD10G1300100.v1.1

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007660
Physical Location & Seq
Forward (+)
38745228 .. 38746256
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1300100.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGGAGGAGCTAGAAAGCGTCACCACCACCAGCCCGACCCAATCGGGCCAACCCGAATCCCAACCCCGAGCCCAGCTCCCCCACTACTGGGGCACCACACCAGAGCAAGAATACTACAATCTCCAGGGCATCAAATCCTCCAAATCCTTCTACACCTCCCCCAGAGGCTTTAAGCTCTTCACCCGCCAATGGCTCCCCCTCCGCCGTCCTCGTGGTATCATCTTCATGGTCCACGGGTACGGCAACGATATCAGCTGGACCTTCCAAGCCACCCCGATCTTCCTCGCCCAAAACGGTTTTGCCTGCTTCGCTCTCGACCTCGAGGGCCACGGCGGCTCCGACGGCCTCCGCGCCTTCGTCCCCGACGTTGGCCTCGTGGTCAACGACTGCCTCTCGTTCTTCAATTTGGTCAAACAGCAGGACCCCCAATTTCAGGGACTCCCCTGTTTCTTATACGGCGAGTCCATGGGCGGCGCGATTTGCCTCCTCATCCACTTCGCCGCCCCGGAGGCGTTCCAAGGCGCGATCTTGGTTGCCCCCATGTGTAAAATCTCCGATAAAGTAAAACCCACATGGCCGATTCCGGAAATTCTGACGTTCGTGGCGGGATACTTGCGGAGATTGGCGATCGTCCCGACCGCCGACCTGATGCGGAAGTCCGTTAAGGTGGAGGAGAAGAAGATCGTGGCCGATATGAATCCGTCGAGATACCGGGGGAAGCCGAGGCTGGGGACGGTGGTGGAACTGCTGAGAGTGACGCAGCACGTGAGCGAGAGATTGGGGGATGTGAGTCTTCCGTTTATTGTGTTGCACGGCAGCGCAGATGTGGTGACGGACCCGGAGGTGAGCAAGGCGTTGTATGCGGCGGCGAAGAGCGAGGATAAGACGTTGAAGATTTATGAGGGGATGATGCATTCGCTGCTGTTTGGGGAGACTGATGAGAATGTGGAGGTTGTGAGGGGAGATATTTTGGGTTGGTTGAATCATCGGTGCGGAGGAGGCGGCCGCGGCGGCGAAAGAGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

343

Amino Acids

37.93

Weight (kDa)

6.27

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 69 - 311 4.2e-56 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 74 - 309 5.5e-13 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 74 - 191 5e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 320
AccB1I GGYRCC 1 cut(s) 92
AccII CGCG 4 cut(s) 351, 476, 524, 1008
AccIII TCCGGA 1 cut(s) 583
AcoI YGGCCR 3 cut(s) 575, 687, 1003
AcsI RAATTY 1 cut(s) 588
AcvI CACGTG 1 cut(s) 766
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 6 cut(s) 87, 88, 189, 368, 433, 728
AgsI TTSAA 3 cut(s) 403, 892, 982
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 4 cut(s) 10, 76, 175, 255
AluI AGCT 4 cut(s) 10, 76, 175, 255
Alw26I GTCTC 1 cut(s) 926
Ama87I CYCGRG 2 cut(s) 66, 320
Aor13HI TCCGGA 1 cut(s) 583
AoxI GGCC 7 cut(s) 46, 325, 343, 370, 575, 687, 1003
ApeKI GCWGC 3 cut(s) 760, 816, 919
ApoI RAATTY 1 cut(s) 588
AspLEI GCGC 4 cut(s) 353, 476, 524, 821
AspS9I GGNCC 6 cut(s) 46, 229, 258, 325, 421, 835
AsuC2I CCSGG 3 cut(s) 506, 713, 839
AsuHPI GGTGA 4 cut(s) 13, 172, 841, 856
AvaI CYCGRG 2 cut(s) 66, 320
AvaII GGWCC 4 cut(s) 229, 258, 421, 835
BaeGI GKGCMC 1 cut(s) 95
BanI GGYRCC 1 cut(s) 92
BanII GRGCYC 1 cut(s) 73
BauI CACGAG 2 cut(s) 210, 374
BbrPI CACGTG 1 cut(s) 766
BbsI GAAGAC 1 cut(s) 785
BbvI GCAGC 3 cut(s) 772, 828, 906
BceAI ACGGC 6 cut(s) 189, 256, 346, 358, 472, 829
BciT130I CCWGG 1 cut(s) 125
BciVI GTATCC 1 cut(s) 602
BcnI CCSGG 3 cut(s) 506, 713, 839
BcoDI GTCTC 1 cut(s) 926
BfaI CTAG 1 cut(s) 11
BfuI GTATCC 1 cut(s) 602
BglI GCCNNNNNGGC 3 cut(s) 333, 509, 1011
Bme1390I CCNGG 4 cut(s) 125, 506, 713, 839
Bme18I GGWCC 4 cut(s) 229, 258, 421, 835
BmeT110I CYCGRG 2 cut(s) 66, 320
BmgT120I GGNCC 6 cut(s) 46, 229, 258, 325, 421, 835
BmiI GGNNCC 5 cut(s) 94, 194, 337, 423, 837
BmrFI CCNGG 4 cut(s) 125, 506, 713, 839
BmrI ACTGGG 1 cut(s) 97
BmsI GCATC 3 cut(s) 138, 639, 900
BmuI ACTGGG 1 cut(s) 97
BpiI GAAGAC 1 cut(s) 785
BplI GAGNNNNNCTC 2 cut(s) 60, 92
BpmI CTGGAG 1 cut(s) 107
BpuMI CCSGG 3 cut(s) 506, 713, 839
BsaAI YACGTR 1 cut(s) 766
BsaBI GATNNNNATC 1 cut(s) 696
BsaJI CCNNGG 9 cut(s) 124, 232, 328, 465, 504, 517, 712, 722, 1006
BsaWI WCCGGW 1 cut(s) 583
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 6 cut(s) 87, 88, 189, 368, 433, 728
Bse1I ACTGG 1 cut(s) 92
Bse8I GATNNNNATC 1 cut(s) 696
BseAI TCCGGA 1 cut(s) 583
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 9 cut(s) 124, 232, 328, 465, 504, 517, 712, 722, 1006
BseGI GGATG 3 cut(s) 489, 790, 912
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 6 cut(s) 87, 88, 189, 368, 433, 728
BseMII CTCAG 1 cut(s) 740
BseNI ACTGG 1 cut(s) 92
BseRI GAGGAG 4 cut(s) 20, 476, 686, 1011
BseSI GKGCMC 1 cut(s) 95
BseX3I CGGCCG 1 cut(s) 1003
BseXI GCAGC 3 cut(s) 772, 828, 906
BseYI CCCAGC 2 cut(s) 72, 727
Bsh1236I CGCG 4 cut(s) 351, 476, 524, 1008
Bsh1285I CGRYCG 3 cut(s) 630, 639, 1006
BshFI GGCC 7 cut(s) 48, 327, 345, 372, 577, 689, 1005
BshNI GGYRCC 1 cut(s) 92
BsiEI CGRYCG 3 cut(s) 630, 639, 1006
BsiHKCI CYCGRG 2 cut(s) 66, 320
BsiSI CCGG 4 cut(s) 506, 584, 712, 839
BslFI GGGAC 4 cut(s) 344, 450, 617, 745
BslI CCNNNNNNNGG 6 cut(s) 87, 88, 189, 368, 433, 728
BsmAI GTCTC 1 cut(s) 926
BsmFI GGGAC 4 cut(s) 344, 450, 617, 745
BsmI GAATGC 1 cut(s) 913
BsnI GGCC 7 cut(s) 48, 327, 345, 372, 577, 689, 1005
BsoBI CYCGRG 2 cut(s) 66, 320
Bsp1286I GDGCHC 2 cut(s) 73, 95
Bsp13I TCCGGA 1 cut(s) 583
Bsp143I GATC 4 cut(s) 276, 525, 627, 681
Bsp19I CCATGG 1 cut(s) 465
BspANI GGCC 7 cut(s) 48, 327, 345, 372, 577, 689, 1005
BspCNI CTCAG 1 cut(s) 741
BspEI TCCGGA 1 cut(s) 583
BspFNI CGCG 4 cut(s) 351, 476, 524, 1008
BspLI GGNNCC 5 cut(s) 94, 194, 337, 423, 837
BspQI GCTCTTC 2 cut(s) 182, 866
BspT107I GGYRCC 1 cut(s) 92
BsrI ACTGG 1 cut(s) 92
BssECI CCNNGG 9 cut(s) 124, 232, 328, 465, 504, 517, 712, 722, 1006
BssMI GATC 4 cut(s) 276, 525, 627, 681
BssSI CACGAG 2 cut(s) 210, 374
BssT1I CCWWGG 2 cut(s) 465, 517
Bst2BI CACGAG 2 cut(s) 210, 374
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 296, 736
Bst6I CTCTTC 2 cut(s) 182, 866
BstAPI GCANNNNNTGC 1 cut(s) 919
BstBAI YACGTR 1 cut(s) 766
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 1 cut(s) 749
BstDSI CCRYGG 4 cut(s) 232, 328, 465, 1006
BstF5I GGATG 3 cut(s) 489, 790, 912
BstFNI CGCG 4 cut(s) 351, 476, 524, 1008
BstHHI GCGC 4 cut(s) 353, 476, 524, 821
BstKTI GATC 4 cut(s) 279, 528, 630, 684
BstMAI GTCTC 1 cut(s) 926
BstMBI GATC 4 cut(s) 276, 525, 627, 681
BstMCI CGRYCG 3 cut(s) 630, 639, 1006
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 4 cut(s) 123, 504, 711, 837
BstSLI GKGCMC 1 cut(s) 95
BstUI CGCG 4 cut(s) 351, 476, 524, 1008
BstV1I GCAGC 3 cut(s) 772, 828, 906
BstV2I GAAGAC 1 cut(s) 785
BstZI CGGCCG 1 cut(s) 1003
BsuI GTATCC 1 cut(s) 602
BsuRI GGCC 7 cut(s) 48, 327, 345, 372, 577, 689, 1005
BtgI CCRYGG 4 cut(s) 232, 328, 465, 1006
BtsCI GGATG 3 cut(s) 489, 790, 912
Cac8I GCNNGC 1 cut(s) 304
CciNI GCGGCCGC 1 cut(s) 1003
CfoI GCGC 4 cut(s) 353, 476, 524, 821
Cfr13I GGNCC 6 cut(s) 46, 229, 258, 325, 421, 835
Cfr42I CCGCGG 1 cut(s) 1009
CseI GACGC 2 cut(s) 7, 766
Csp6I GTAC 1 cut(s) 238
CviAII CATG 4 cut(s) 226, 466, 541, 573
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 1 cut(s) 749
DpnI GATC 4 cut(s) 278, 527, 629, 683
DpnII GATC 4 cut(s) 276, 525, 627, 681
EaeI YGGCCR 3 cut(s) 575, 687, 1003
EagI CGGCCG 1 cut(s) 1003
Eam1104I CTCTTC 2 cut(s) 182, 866
EarI CTCTTC 2 cut(s) 182, 866
EciI GGCGGA 1 cut(s) 191
EclXI CGGCCG 1 cut(s) 1003
Eco130I CCWWGG 2 cut(s) 465, 517
Eco24I GRGCYC 1 cut(s) 73
Eco32I GATATC 1 cut(s) 250
Eco47I GGWCC 4 cut(s) 229, 258, 421, 835
Eco52I CGGCCG 1 cut(s) 1003
Eco72I CACGTG 1 cut(s) 766
Eco88I CYCGRG 2 cut(s) 66, 320
EcoO109I RGGNCCY 1 cut(s) 421
EcoRII CCWGG 1 cut(s) 123
EcoRV GATATC 1 cut(s) 250
EcoT14I CCWWGG 2 cut(s) 465, 517
EcoT22I ATGCAT 1 cut(s) 915
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 2 cut(s) 465, 517
FaeI CATG 4 cut(s) 229, 469, 544, 576
FaiI YATR 8 cut(s) 227, 454, 467, 542, 574, 695, 861, 900
FaqI GGGAC 4 cut(s) 344, 450, 617, 745
FatI CATG 4 cut(s) 225, 465, 540, 572
FauI CCCGC 2 cut(s) 191, 598
FokI GGATG 3 cut(s) 476, 797, 919
FriOI GRGCYC 1 cut(s) 73
FspBI CTAG 1 cut(s) 11
GlaI GCGC 4 cut(s) 352, 475, 523, 820
GsaI CCCAGC 2 cut(s) 76, 731
GsuI CTGGAG 1 cut(s) 107
HaeIII GGCC 7 cut(s) 48, 327, 345, 372, 577, 689, 1005
HapII CCGG 4 cut(s) 506, 584, 712, 839
HgaI GACGC 2 cut(s) 7, 766
HhaI GCGC 4 cut(s) 353, 476, 524, 821
Hin1II CATG 4 cut(s) 229, 469, 544, 576
Hin6I GCGC 4 cut(s) 351, 474, 522, 819
HinP1I GCGC 4 cut(s) 351, 474, 522, 819
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 7 cut(s) 56, 438, 461, 580, 697, 790, 982
HpaII CCGG 4 cut(s) 506, 584, 712, 839
HphI GGTGA 4 cut(s) 13, 172, 841, 856
Hpy166II GTNNAC 2 cut(s) 232, 382
Hpy188I TCNGA 3 cut(s) 340, 556, 594
Hpy188III TCNNGA 4 cut(s) 314, 584, 634, 705
Hpy8I GTNNAC 2 cut(s) 232, 382
Hpy99I CGWCG 3 cut(s) 344, 368, 706
HpyAV CCTTC 3 cut(s) 157, 271, 364
HpyCH4III ACNGT 2 cut(s) 296, 736
HpyCH4IV ACGT 4 cut(s) 366, 596, 765, 887
HpyCH4V TGCA 2 cut(s) 811, 913
HpyF3I CTNAG 1 cut(s) 749
HpySE526I ACGT 4 cut(s) 366, 596, 765, 887
Hsp92II CATG 4 cut(s) 229, 469, 544, 576
HspAI GCGC 4 cut(s) 351, 474, 522, 819
Kpn2I TCCGGA 1 cut(s) 583
KspI CCGCGG 1 cut(s) 1009
Kzo9I GATC 4 cut(s) 276, 525, 627, 681
LguI GCTCTTC 2 cut(s) 182, 866
LmnI GCTCC 4 cut(s) 7, 81, 198, 341
Lsp1109I GCAGC 3 cut(s) 772, 828, 906
LweI GCATC 3 cut(s) 138, 639, 900
MaeI CTAG 1 cut(s) 11
MaeII ACGT 4 cut(s) 366, 596, 765, 887
MaeIII GTNAC 3 cut(s) 19, 754, 829
MalI GATC 4 cut(s) 278, 527, 629, 683
MboI GATC 4 cut(s) 276, 525, 627, 681
MboII GAAGA 9 cut(s) 169, 214, 271, 391, 688, 691, 785, 883, 904
MhlI GDGCHC 2 cut(s) 73, 95
MluCI AATT 3 cut(s) 403, 428, 588
MlyI GAGTC 3 cut(s) 432, 470, 799
MmeI TCCRAC 1 cut(s) 363
Mph1103I ATGCAT 1 cut(s) 915
MroI TCCGGA 1 cut(s) 583
MseI TTAA 2 cut(s) 171, 663
MslI CAYNNNNRTG 1 cut(s) 224
MspA1I CMGCKG 2 cut(s) 255, 1008
MspI CCGG 4 cut(s) 506, 584, 712, 839
MspR9I CCNGG 4 cut(s) 125, 506, 713, 839
Mva1269I GAATGC 1 cut(s) 913
MvaI CCWGG 1 cut(s) 125
MvnI CGCG 4 cut(s) 351, 476, 524, 1008
NciI CCSGG 3 cut(s) 506, 713, 839
NcoI CCATGG 1 cut(s) 465
NdeII GATC 4 cut(s) 276, 525, 627, 681
NlaIII CATG 4 cut(s) 229, 469, 544, 576
NlaIV GGNNCC 5 cut(s) 94, 194, 337, 423, 837
NmeAIII GCCGAG 1 cut(s) 747
NmuCI GTSAC 3 cut(s) 19, 754, 829
NotI GCGGCCGC 1 cut(s) 1003
NsiI ATGCAT 1 cut(s) 915
PaeR7I CTCGAG 1 cut(s) 320
PciSI GCTCTTC 2 cut(s) 182, 866
PcsI WCGNNNNNNNCGW 4 cut(s) 336, 363, 372, 381
PctI GAATGC 1 cut(s) 913
PfeI GAWTC 4 cut(s) 56, 580, 697, 982
Ple19I CGATCG 1 cut(s) 630
PleI GAGTC 3 cut(s) 432, 469, 798
PmaCI CACGTG 1 cut(s) 766
PmlI CACGTG 1 cut(s) 766
PpsI GAGTC 3 cut(s) 432, 469, 798
Ppu21I YACGTR 1 cut(s) 766
PpuMI RGGWCCY 1 cut(s) 421
Psp5II RGGWCCY 1 cut(s) 421
Psp6I CCWGG 1 cut(s) 123
PspCI CACGTG 1 cut(s) 766
PspFI CCCAGC 2 cut(s) 72, 727
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 5 cut(s) 94, 194, 337, 423, 837
PspPI GGNCC 6 cut(s) 46, 229, 258, 325, 421, 835
PspPPI RGGWCCY 1 cut(s) 421
PspXI VCTCGAGB 1 cut(s) 320
PvuI CGATCG 1 cut(s) 630
PvuII CAGCTG 1 cut(s) 255
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 1 cut(s) 224
SacII CCGCGG 1 cut(s) 1009
SapI GCTCTTC 2 cut(s) 182, 866
SaqAI TTAA 2 cut(s) 171, 663
Sau3AI GATC 4 cut(s) 276, 525, 627, 681
Sau96I GGNCC 6 cut(s) 46, 229, 258, 325, 421, 835
SchI GAGTC 3 cut(s) 432, 470, 799
ScrFI CCNGG 4 cut(s) 125, 506, 713, 839
SduI GDGCHC 2 cut(s) 73, 95
SfaNI GCATC 3 cut(s) 138, 639, 900
Sfr274I CTCGAG 1 cut(s) 320
Sfr303I CCGCGG 1 cut(s) 1009
SgrBI CCGCGG 1 cut(s) 1009
SinI GGWCC 4 cut(s) 229, 258, 421, 835
SlaI CTCGAG 1 cut(s) 320
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 3 cut(s) 403, 428, 588
SspMI CTAG 1 cut(s) 11
StyD4I CCNGG 4 cut(s) 123, 504, 711, 837
StyI CCWWGG 2 cut(s) 465, 517
TaaI ACNGT 2 cut(s) 296, 736
TaiI ACGT 4 cut(s) 369, 599, 768, 890
TaqI TCGA 3 cut(s) 315, 321, 704
TasI AATT 3 cut(s) 403, 428, 588
TauI GCSGC 9 cut(s) 336, 474, 503, 866, 869, 1005, 1008, 1011, 1014
TfiI GAWTC 4 cut(s) 56, 580, 697, 982
Tru1I TTAA 2 cut(s) 171, 663
Tru9I TTAA 2 cut(s) 171, 663
TseFI GTSAC 3 cut(s) 19, 754, 829
TseI GCWGC 3 cut(s) 760, 816, 919
Tsp45I GTSAC 3 cut(s) 19, 754, 829
TspDTI ATGAA 2 cut(s) 214, 710
TspGWI ACGGA 4 cut(s) 649, 690, 786, 848
VpaK11BI GGWCC 4 cut(s) 229, 258, 421, 835
XapI RAATTY 1 cut(s) 588
XhoI CTCGAG 1 cut(s) 320
XspI CTAG 1 cut(s) 11
Zsp2I ATGCAT 1 cut(s) 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.