FvH4_3g08400

OPA3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004304
Physical Location & Seq
Reverse (-)
4908157 .. 4910853
2697 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g08400.t1

Sequence Viewer

Length: 513 bp
ATGGTGCTCCCTCTTGTAAAGCTCGGAACTTTGCTGTTAAAGACTATGAGCAAACCAGTAGCCGCCAGGCTTAAATATGCAGCTGGGAAGCACCCCAGGTTCCGCCAGATTATCGTCAACATGGCTCAGGCAAACCACCGAATCACCACGAAAATGCAAAGAAGCCTCTACAGTCATGCAACTGATGCCGAGATTCGGCCCTTGAATGAAGAGAAGGCTGTGCAGGCTGCTGTGGACCTCATCGGGGAGTTCTTTGTCTTCACGGTTGCAGTAGTTGCTATGATTTTTGAAGTGCAAAGAAGTGCCAGATCAGAAGCCAGAAAAGAGGAAGCACGAAAGCAAGAACTGGAGGCAATGAAGCAAAGAGATGAAGATTTATCAAGAGAAGTGGAACTTCTGAGGAGCAAATATGAAGAACTGGAGAAGCTTGCCAAGGGACAAGGGCTCAGTGGTGTTTTCAATTTTAAGCGTGTTGCTGAAAAAGAAGGTGCCAATTCAGTAAAACCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPA3

Protein Analysis

171

Amino Acids

19.21

Weight (kDa)

9.76

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPA3 PF07047 6 - 126 1.3e-42 Optic atrophy 3 protein (OPA3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 488
AciI CCGC 2 cut(s) 63, 103
AfiI CCNNNNNNNGG 2 cut(s) 195, 244
AgsI TTSAA 3 cut(s) 205, 290, 460
AjnI CCWGG 2 cut(s) 65, 95
AluBI AGCT 4 cut(s) 22, 83, 427, 509
AluI AGCT 4 cut(s) 22, 83, 427, 509
Alw21I GWGCWC 1 cut(s) 9
AoxI GGCC 1 cut(s) 197
ApeKI GCWGC 2 cut(s) 80, 227
AspS9I GGNCC 2 cut(s) 198, 235
AsuHPI GGTGA 1 cut(s) 136
AvaII GGWCC 1 cut(s) 235
BanI GGYRCC 1 cut(s) 488
BanII GRGCYC 1 cut(s) 447
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 2 cut(s) 92, 214
BciT130I CCWGG 2 cut(s) 67, 97
BfmI CTRYAG 1 cut(s) 169
BisI GCNGC 3 cut(s) 63, 81, 228
BlsI GCNGC 3 cut(s) 64, 82, 229
Bme1390I CCNGG 2 cut(s) 67, 97
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 2 cut(s) 198, 235
BmiI GGNNCC 2 cut(s) 101, 490
BmrFI CCNGG 2 cut(s) 67, 97
BmsI GCATC 1 cut(s) 175
BpiI GAAGAC 1 cut(s) 250
BpmI CTGGAG 2 cut(s) 368, 440
Bpu10I CCTNAGC 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 95, 432
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 244
Bse1I ACTGG 3 cut(s) 56, 351, 423
Bse3DI GCAATG 1 cut(s) 360
BseBI CCWGG 2 cut(s) 67, 97
BseDI CCNNGG 2 cut(s) 95, 432
BseLI CCNNNNNNNGG 2 cut(s) 195, 244
BseMI GCAATG 1 cut(s) 360
BseMII CTCAG 3 cut(s) 140, 389, 460
BseNI ACTGG 3 cut(s) 56, 351, 423
BseRI GAGGAG 1 cut(s) 415
BseXI GCAGC 2 cut(s) 92, 214
BseYI CCCAGC 1 cut(s) 83
BsgI GTGCAG 1 cut(s) 242
BshFI GGCC 1 cut(s) 199
BshNI GGYRCC 1 cut(s) 488
BsiHKAI GWGCWC 1 cut(s) 9
BslFI GGGAC 1 cut(s) 450
BslI CCNNNNNNNGG 2 cut(s) 195, 244
BsmFI GGGAC 1 cut(s) 450
BsnI GGCC 1 cut(s) 199
Bsp1286I GDGCHC 2 cut(s) 9, 447
Bsp143I GATC 1 cut(s) 308
BspACI CCGC 2 cut(s) 63, 103
BspANI GGCC 1 cut(s) 199
BspCNI CTCAG 3 cut(s) 139, 390, 459
BspLI GGNNCC 2 cut(s) 101, 490
BspT107I GGYRCC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 360
BsrI ACTGG 3 cut(s) 56, 351, 423
BssECI CCNNGG 2 cut(s) 95, 432
BssMI GATC 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 432
Bst2UI CCWGG 2 cut(s) 67, 97
Bst4CI ACNGT 2 cut(s) 173, 265
Bst6I CTCTTC 1 cut(s) 204
BstAPI GCANNNNNTGC 2 cut(s) 185, 275
BstC8I GCNNGC 2 cut(s) 225, 429
BstDEI CTNAG 3 cut(s) 126, 398, 446
BstKTI GATC 1 cut(s) 311
BstMBI GATC 1 cut(s) 308
BstMWI GCNNNNNNNGC 3 cut(s) 185, 224, 275
BstNI CCWGG 2 cut(s) 67, 97
BstSCI CCNGG 2 cut(s) 65, 95
BstSFI CTRYAG 1 cut(s) 169
BstV1I GCAGC 2 cut(s) 92, 214
BstV2I GAAGAC 1 cut(s) 250
BsuRI GGCC 1 cut(s) 199
BtsIMutI CAGTG 1 cut(s) 454
Cac8I GCNNGC 2 cut(s) 225, 429
Cfr13I GGNCC 2 cut(s) 198, 235
CspCI CAANNNNNGTGG 2 cut(s) 369, 404
CviAII CATG 2 cut(s) 121, 176
DdeI CTNAG 3 cut(s) 126, 398, 446
DpnI GATC 1 cut(s) 310
DpnII GATC 1 cut(s) 308
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
EciI GGCGGA 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 432
Eco24I GRGCYC 1 cut(s) 447
Eco47I GGWCC 1 cut(s) 235
EcoRII CCWGG 2 cut(s) 65, 95
EcoT14I CCWWGG 1 cut(s) 432
EcoT38I GRGCYC 1 cut(s) 447
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 2 cut(s) 124, 179
FaiI YATR 6 cut(s) 47, 78, 122, 177, 281, 411
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 1 cut(s) 450
FatI CATG 2 cut(s) 120, 175
Fnu4HI GCNGC 3 cut(s) 63, 81, 228
FriOI GRGCYC 1 cut(s) 447
Fsp4HI GCNGC 3 cut(s) 63, 81, 228
GluI GCNGC 3 cut(s) 63, 81, 228
GsaI CCCAGC 1 cut(s) 87
GsuI CTGGAG 2 cut(s) 368, 440
HaeIII GGCC 1 cut(s) 199
Hin1II CATG 2 cut(s) 124, 179
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 2 cut(s) 141, 193
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 2 cut(s) 118, 235
Hpy188I TCNGA 3 cut(s) 26, 313, 399
Hpy188III TCNNGA 1 cut(s) 381
Hpy8I GTNNAC 2 cut(s) 118, 235
HpyAV CCTTC 2 cut(s) 208, 479
HpyCH4III ACNGT 2 cut(s) 173, 265
HpyCH4V TGCA 6 cut(s) 80, 157, 179, 223, 269, 295
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 224, 275
HpyF3I CTNAG 3 cut(s) 126, 398, 446
Hsp92II CATG 2 cut(s) 124, 179
Kzo9I GATC 1 cut(s) 308
LmnI GCTCC 2 cut(s) 12, 402
Lsp1109I GCAGC 2 cut(s) 92, 214
LweI GCATC 1 cut(s) 175
MalI GATC 1 cut(s) 310
MboI GATC 1 cut(s) 308
MboII GAAGA 4 cut(s) 221, 250, 383, 425
MhlI GDGCHC 2 cut(s) 9, 447
MluCI AATT 2 cut(s) 460, 493
MnlI CCTC 6 cut(s) 21, 176, 248, 319, 343, 393
MseI TTAA 3 cut(s) 38, 72, 465
MslI CAYNNNNRTG 1 cut(s) 152
MspA1I CMGCKG 1 cut(s) 83
MspR9I CCNGG 2 cut(s) 67, 97
MvaI CCWGG 2 cut(s) 67, 97
MwoI GCNNNNNNNGC 3 cut(s) 185, 224, 275
NdeII GATC 1 cut(s) 308
NlaIII CATG 2 cut(s) 124, 179
NlaIV GGNNCC 2 cut(s) 101, 490
NmeAIII GCCGAG 1 cut(s) 214
PfeI GAWTC 2 cut(s) 141, 193
PkrI GCNGC 3 cut(s) 64, 82, 229
Psp6I CCWGG 2 cut(s) 65, 95
PspFI CCCAGC 1 cut(s) 83
PspGI CCWGG 2 cut(s) 65, 95
PspN4I GGNNCC 2 cut(s) 101, 490
PspPI GGNCC 2 cut(s) 198, 235
PvuII CAGCTG 1 cut(s) 83
RseI CAYNNNNRTG 1 cut(s) 152
SaqAI TTAA 3 cut(s) 38, 72, 465
SatI GCNGC 3 cut(s) 63, 81, 228
Sau3AI GATC 1 cut(s) 308
Sau96I GGNCC 2 cut(s) 198, 235
ScrFI CCNGG 2 cut(s) 67, 97
SduI GDGCHC 2 cut(s) 9, 447
SetI ASST 7 cut(s) 24, 85, 101, 240, 429, 490, 511
SfaNI GCATC 1 cut(s) 175
SfcI CTRYAG 1 cut(s) 169
SinI GGWCC 1 cut(s) 235
SmiMI CAYNNNNRTG 1 cut(s) 152
Sse9I AATT 2 cut(s) 460, 493
SsiI CCGC 2 cut(s) 63, 103
StyD4I CCNGG 2 cut(s) 65, 95
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 2 cut(s) 173, 265
TasI AATT 2 cut(s) 460, 493
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 2 cut(s) 141, 193
Tru1I TTAA 3 cut(s) 38, 72, 465
Tru9I TTAA 3 cut(s) 38, 72, 465
TscAI CASTG 1 cut(s) 454
TseI GCWGC 2 cut(s) 80, 227
TspDTI ATGAA 4 cut(s) 222, 371, 384, 426
TspRI CASTG 1 cut(s) 454
VpaK11BI GGWCC 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.