MD05G1308400.v1.1

OPA3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004304
Physical Location & Seq
Reverse (-)
43947484 .. 43950452
2969 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1308400.v1.1.491

Sequence Viewer

Length: 510 bp
ATGGTGCTTCCGCTCCTGAAGCTCGGGACTCTTGCCATCAAAACTCTCAGCAAACCCGTCGCCGCCCGACTCAAACATCAAGCTGCAGTGCATCCCAGGTTCCGCCAGTTAATCGTCAGCATGGCACAGGCAAACCATCGAATTACCACGAGGATGCAAAGGCGCCTATACAGTCATGCGACCGATGTTGAGATTCGCCCTTTGAATGAGGAGAAGGCTGTGCAGGCTGCTGTTGACCTTATGGGGGAGCTCTTTGTCTTCTCGGTTGCAACTGTTGCTTTGATATTTGAAGTACAAAGAAGTGCTAGATCAGAAGCGAGAAAGGAGGAAGCACGCAAGCAAGAACTGGAGGCACTCAAGCAAAGAGACGAAGCCTTATCCAGAGAAGTAGAACTTCTTAAGGAAAAATTTGAAGAACTGGAGAAGCTTGCCAGGGGACGAGGGCTTAGCGGTATTATCAGTTTCAAGCGCCCTGATACAGAATCTGCAAAGTCAGTAAAGCCAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPA3

Protein Analysis

170

Amino Acids

19.11

Weight (kDa)

10.04

Isoelectric Point (pI)

31.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPA3 PF07047 6 - 126 8.7e-44 Optic atrophy 3 protein (OPA3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 162
AccBSI CCGCTC 1 cut(s) 13
AciI CCGC 4 cut(s) 11, 63, 103, 450
AcsI RAATTY 1 cut(s) 407
AcuI CTGAAG 1 cut(s) 38
AcyI GRCGYC 1 cut(s) 163
AfaI GTAC 1 cut(s) 294
AfiI CCNNNNNNNGG 1 cut(s) 244
AflII CTTAAG 1 cut(s) 398
AgsI TTSAA 4 cut(s) 205, 290, 413, 466
AjnI CCWGG 2 cut(s) 95, 431
AluBI AGCT 4 cut(s) 22, 83, 250, 427
AluI AGCT 4 cut(s) 22, 83, 250, 427
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 1 cut(s) 360
AlwNI CAGNNNCTG 1 cut(s) 485
Ama87I CYCGRG 1 cut(s) 23
ApeKI GCWGC 2 cut(s) 83, 227
ApoI RAATTY 1 cut(s) 407
AspLEI GCGC 2 cut(s) 165, 471
AvaI CYCGRG 1 cut(s) 23
BanI GGYRCC 1 cut(s) 162
BanII GRGCYC 1 cut(s) 252
BauI CACGAG 1 cut(s) 148
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 2 cut(s) 70, 214
BccI CCATC 2 cut(s) 44, 144
BcgI CGANNNNNNTGC 2 cut(s) 40, 74
BciT130I CCWGG 2 cut(s) 97, 433
BcoDI GTCTC 1 cut(s) 360
BfaI CTAG 1 cut(s) 306
BfmI CTRYAG 1 cut(s) 84
BfoI RGCGCY 2 cut(s) 166, 472
BfrI CTTAAG 1 cut(s) 398
BisI GCNGC 3 cut(s) 63, 84, 228
BlpI GCTNAGC 1 cut(s) 446
BlsI GCNGC 3 cut(s) 64, 85, 229
Bme1390I CCNGG 2 cut(s) 97, 433
BmeT110I CYCGRG 1 cut(s) 23
BmiI GGNNCC 2 cut(s) 101, 164
BmrFI CCNGG 2 cut(s) 97, 433
BmsI GCATC 2 cut(s) 100, 144
BpiI GAAGAC 1 cut(s) 250
BpmI CTGGAG 2 cut(s) 368, 440
Bpu1102I GCTNAGC 1 cut(s) 446
BpuEI CTTGAG 1 cut(s) 341
BsaHI GRCGYC 1 cut(s) 163
BsaJI CCNNGG 2 cut(s) 95, 432
Bsc4I CCNNNNNNNGG 1 cut(s) 244
Bse1I ACTGG 3 cut(s) 106, 351, 423
BseBI CCWGG 2 cut(s) 97, 433
BseDI CCNNGG 2 cut(s) 95, 432
BseGI GGATG 2 cut(s) 91, 159
BseLI CCNNNNNNNGG 1 cut(s) 244
BseMII CTCAG 1 cut(s) 61
BseNI ACTGG 3 cut(s) 106, 351, 423
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 2 cut(s) 70, 214
BsgI GTGCAG 1 cut(s) 242
Bsh1285I CGRYCG 1 cut(s) 183
BshNI GGYRCC 1 cut(s) 162
BsiEI CGRYCG 1 cut(s) 183
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 1 cut(s) 23
BslFI GGGAC 2 cut(s) 40, 450
BslI CCNNNNNNNGG 1 cut(s) 244
BsmAI GTCTC 1 cut(s) 360
BsmBI CGTCTC 1 cut(s) 360
BsmFI GGGAC 2 cut(s) 40, 450
BsoBI CYCGRG 1 cut(s) 23
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 1 cut(s) 308
Bsp1720I GCTNAGC 1 cut(s) 446
BspACI CCGC 4 cut(s) 11, 63, 103, 450
BspCNI CTCAG 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 101, 164
BspMAI CTGCAG 1 cut(s) 88
BspT107I GGYRCC 1 cut(s) 162
BspTI CTTAAG 1 cut(s) 398
BsrBI CCGCTC 1 cut(s) 13
BsrI ACTGG 3 cut(s) 106, 351, 423
BssECI CCNNGG 2 cut(s) 95, 432
BssMI GATC 1 cut(s) 308
BssNI GRCGYC 1 cut(s) 163
BssSI CACGAG 1 cut(s) 148
Bst2BI CACGAG 1 cut(s) 148
Bst2UI CCWGG 2 cut(s) 97, 433
Bst4CI ACNGT 2 cut(s) 173, 274
BstACI GRCGYC 1 cut(s) 163
BstAFI CTTAAG 1 cut(s) 398
BstAPI GCANNNNNTGC 1 cut(s) 275
BstC8I GCNNGC 5 cut(s) 225, 334, 338, 429, 504
BstDEI CTNAG 2 cut(s) 47, 446
BstF5I GGATG 2 cut(s) 91, 159
BstH2I RGCGCY 2 cut(s) 166, 472
BstHHI GCGC 2 cut(s) 165, 471
BstKTI GATC 1 cut(s) 311
BstMAI GTCTC 1 cut(s) 360
BstMBI GATC 1 cut(s) 308
BstMCI CGRYCG 1 cut(s) 183
BstMWI GCNNNNNNNGC 3 cut(s) 19, 224, 275
BstNI CCWGG 2 cut(s) 97, 433
BstSCI CCNGG 2 cut(s) 95, 431
BstSFI CTRYAG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 70, 214
BstV2I GAAGAC 1 cut(s) 250
BtsCI GGATG 2 cut(s) 91, 159
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 93
Cac8I GCNNGC 5 cut(s) 225, 334, 338, 429, 504
CaiI CAGNNNCTG 1 cut(s) 485
CfoI GCGC 2 cut(s) 165, 471
Csp6I GTAC 1 cut(s) 293
CviAII CATG 2 cut(s) 121, 176
CviQI GTAC 1 cut(s) 293
DdeI CTNAG 2 cut(s) 47, 446
DinI GGCGCC 1 cut(s) 164
DpnI GATC 1 cut(s) 310
DpnII GATC 1 cut(s) 308
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 1 cut(s) 38
Eco88I CYCGRG 1 cut(s) 23
EcoICRI GAGCTC 1 cut(s) 250
EcoRII CCWGG 2 cut(s) 95, 431
EcoT38I GRGCYC 1 cut(s) 252
EgeI GGCGCC 1 cut(s) 164
EheI GGCGCC 1 cut(s) 164
Esp3I CGTCTC 1 cut(s) 360
FaeI CATG 2 cut(s) 124, 179
FaiI YATR 4 cut(s) 122, 169, 177, 242
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 2 cut(s) 40, 450
FatI CATG 2 cut(s) 120, 175
Fnu4HI GCNGC 3 cut(s) 63, 84, 228
FokI GGATG 2 cut(s) 78, 166
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 3 cut(s) 63, 84, 228
FspBI CTAG 1 cut(s) 306
GlaI GCGC 2 cut(s) 164, 470
GluI GCNGC 3 cut(s) 63, 84, 228
GsuI CTGGAG 2 cut(s) 368, 440
HaeII RGCGCY 2 cut(s) 166, 472
HhaI GCGC 2 cut(s) 165, 471
Hin1I GRCGYC 1 cut(s) 163
Hin1II CATG 2 cut(s) 124, 179
Hin6I GCGC 2 cut(s) 163, 469
HinP1I GCGC 2 cut(s) 163, 469
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 4 cut(s) 28, 69, 193, 482
Hpy166II GTNNAC 1 cut(s) 235
Hpy188I TCNGA 1 cut(s) 313
Hpy188III TCNNGA 3 cut(s) 16, 25, 381
Hpy8I GTNNAC 1 cut(s) 235
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 2 cut(s) 173, 274
HpyCH4V TGCA 6 cut(s) 86, 91, 157, 223, 269, 488
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 224, 275
HpyF3I CTNAG 2 cut(s) 47, 446
Hsp92I GRCGYC 1 cut(s) 163
Hsp92II CATG 2 cut(s) 124, 179
HspAI GCGC 2 cut(s) 163, 469
KasI GGCGCC 1 cut(s) 162
Kzo9I GATC 1 cut(s) 308
LmnI GCTCC 2 cut(s) 18, 247
Lsp1109I GCAGC 2 cut(s) 70, 214
LweI GCATC 2 cut(s) 100, 144
MaeI CTAG 1 cut(s) 306
MalI GATC 1 cut(s) 310
MbiI CCGCTC 1 cut(s) 13
MboI GATC 1 cut(s) 308
MboII GAAGA 2 cut(s) 250, 425
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 2 cut(s) 141, 407
Mly113I GGCGCC 1 cut(s) 163
MlyI GAGTC 2 cut(s) 22, 63
MnlI CCTC 5 cut(s) 144, 202, 319, 343, 434
MseI TTAA 2 cut(s) 110, 399
MslI CAYNNNNRTG 1 cut(s) 152
MspCI CTTAAG 1 cut(s) 398
MspR9I CCNGG 2 cut(s) 97, 433
MvaI CCWGG 2 cut(s) 97, 433
MwoI GCNNNNNNNGC 3 cut(s) 19, 224, 275
NarI GGCGCC 1 cut(s) 163
NdeII GATC 1 cut(s) 308
NlaIII CATG 2 cut(s) 124, 179
NlaIV GGNNCC 2 cut(s) 101, 164
PfeI GAWTC 2 cut(s) 193, 482
PkrI GCNGC 3 cut(s) 64, 85, 229
PleI GAGTC 2 cut(s) 22, 63
PluTI GGCGCC 1 cut(s) 166
PpsI GAGTC 2 cut(s) 22, 63
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 95, 431
PspGI CCWGG 2 cut(s) 95, 431
PspN4I GGNNCC 2 cut(s) 101, 164
PstI CTGCAG 1 cut(s) 88
PstNI CAGNNNCTG 1 cut(s) 485
RsaI GTAC 1 cut(s) 294
RsaNI GTAC 1 cut(s) 293
RseI CAYNNNNRTG 1 cut(s) 152
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 2 cut(s) 110, 399
SatI GCNGC 3 cut(s) 63, 84, 228
Sau3AI GATC 1 cut(s) 308
SchI GAGTC 2 cut(s) 22, 63
ScrFI CCNGG 2 cut(s) 97, 433
SduI GDGCHC 1 cut(s) 252
SetI ASST 6 cut(s) 24, 85, 101, 240, 252, 429
SfaNI GCATC 2 cut(s) 100, 144
SfcI CTRYAG 1 cut(s) 84
SfoI GGCGCC 1 cut(s) 164
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 2 cut(s) 356, 398
SmoI CTYRAG 2 cut(s) 356, 398
Sse9I AATT 2 cut(s) 141, 407
SsiI CCGC 4 cut(s) 11, 63, 103, 450
SspDI GGCGCC 1 cut(s) 162
SspMI CTAG 1 cut(s) 306
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 95, 431
TaaI ACNGT 2 cut(s) 173, 274
TaqI TCGA 1 cut(s) 139
TaqII GACCGA 1 cut(s) 197
TasI AATT 2 cut(s) 141, 407
TatI WGTACW 1 cut(s) 292
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 2 cut(s) 193, 482
Tru1I TTAA 2 cut(s) 110, 399
Tru9I TTAA 2 cut(s) 110, 399
TscAI CASTG 1 cut(s) 93
TseI GCWGC 2 cut(s) 83, 227
TspRI CASTG 1 cut(s) 93
Vha464I CTTAAG 1 cut(s) 398
XapI RAATTY 1 cut(s) 407
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.