RchiOBHm_Chr2g0128211

Optic atrophy 3 protein (OPA3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004304
Physical Location & Seq
Forward (+)
43159379 .. 43164097
4719 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50001

Sequence Viewer

Length: 531 bp
ATGATCCTACCAGCGGTTAAGTTAGGAACCCTTGCCCTGAAAACCTTCTGCAAACCCATTGCCAATCGCCTCAAGAAAGAGGCTGGCTTGCACCCCAGGTTTAGGCAGTTCATCATCAACATTGCCCAGGCAAATCATCGATTTACAACAAATGTGCAAAGACGGATTTATGGTCATTCAACTGATGTTGCGATTCGTCCACTGAACGAGGAGAAAGCTGTCCAAGCTGCTGCAGAACTTCTTGGGGAGCTCTTTGTGTTCACGGTTGCAGGAACTGCACTTATTTTTGAAGTCCAAAGAAGTTCAAGATCAGAAGCAAGAAAGGAGGAGCTGCGTAAACAGGAATTACAGGCAATGAAACAAAGAGATGAAGAGCTAGAAAGAGAAGTTCAGCTGCTTAAGAAGAAGCTTGAAGAGCTGGAACAGCTTGCAAAGGGTAGAGGAATTGCTGGCCTTTTCAGTTTCAGGCATGCTCAAGCTACTAATAATGGAAACCGAAATCCCAGCTTGACCCCAGAAAAGATCGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPA3

Protein Analysis

176

Amino Acids

19.98

Weight (kDa)

10.05

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPA3 PF07047 6 - 126 1.7e-44 Optic atrophy 3 protein (OPA3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 14
AfiI CCNNNNNNNGG 2 cut(s) 13, 102
AflII CTTAAG 1 cut(s) 398
AgsI TTSAA 4 cut(s) 180, 290, 306, 413
AjnI CCWGG 2 cut(s) 95, 126
Alw21I GWGCWC 1 cut(s) 252
AlwNI CAGNNNCTG 1 cut(s) 275
AoxI GGCC 1 cut(s) 451
ApeKI GCWGC 4 cut(s) 227, 230, 331, 394
Asp700I GAANNNNTTC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 4 cut(s) 214, 217, 318, 381
BciT130I CCWGG 2 cut(s) 97, 128
BfaI CTAG 1 cut(s) 377
BfmI CTRYAG 1 cut(s) 231
BfrI CTTAAG 1 cut(s) 398
BisI GCNGC 4 cut(s) 228, 231, 332, 395
BlsI GCNGC 4 cut(s) 229, 232, 333, 396
Bme1390I CCNGG 2 cut(s) 97, 128
BmiI GGNNCC 1 cut(s) 28
BmrFI CCNGG 2 cut(s) 97, 128
BpuEI CTTGAG 2 cut(s) 56, 459
Bsa29I ATCGAT 1 cut(s) 139
BsaJI CCNNGG 2 cut(s) 95, 126
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 102
Bse3DI GCAATG 3 cut(s) 57, 120, 360
BseBI CCWGG 2 cut(s) 97, 128
BseCI ATCGAT 1 cut(s) 139
BseDI CCNNGG 2 cut(s) 95, 126
BseLI CCNNNNNNNGG 2 cut(s) 13, 102
BseMI GCAATG 3 cut(s) 57, 120, 360
BseRI GAGGAG 2 cut(s) 224, 341
BseXI GCAGC 4 cut(s) 214, 217, 318, 381
BseYI CCCAGC 1 cut(s) 503
BsgI GTGCAG 1 cut(s) 261
BshFI GGCC 1 cut(s) 453
BshVI ATCGAT 1 cut(s) 139
BsiHKAI GWGCWC 1 cut(s) 252
BslI CCNNNNNNNGG 2 cut(s) 13, 102
BsnI GGCC 1 cut(s) 453
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 3 cut(s) 3, 308, 522
BspACI CCGC 1 cut(s) 14
BspANI GGCC 1 cut(s) 453
BspDI ATCGAT 1 cut(s) 139
BspLI GGNNCC 1 cut(s) 28
BspMAI CTGCAG 1 cut(s) 235
BspQI GCTCTTC 2 cut(s) 366, 408
BspTI CTTAAG 1 cut(s) 398
BsrDI GCAATG 3 cut(s) 57, 120, 360
BssECI CCNNGG 2 cut(s) 95, 126
BssMI GATC 3 cut(s) 3, 308, 522
Bst2UI CCWGG 2 cut(s) 97, 128
Bst4CI ACNGT 1 cut(s) 265
Bst6I CTCTTC 2 cut(s) 366, 408
BstAFI CTTAAG 1 cut(s) 398
BstAPI GCANNNNNTGC 1 cut(s) 275
BstC8I GCNNGC 5 cut(s) 85, 89, 429, 451, 471
BstKTI GATC 3 cut(s) 6, 311, 525
BstMBI GATC 3 cut(s) 3, 308, 522
BstMWI GCNNNNNNNGC 4 cut(s) 224, 275, 415, 424
BstNI CCWGG 2 cut(s) 97, 128
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 2 cut(s) 95, 126
BstSFI CTRYAG 1 cut(s) 231
BstV1I GCAGC 4 cut(s) 214, 217, 318, 381
Bsu15I ATCGAT 1 cut(s) 139
BsuRI GGCC 1 cut(s) 453
BsuTUI ATCGAT 1 cut(s) 139
BtsIMutI CAGTG 1 cut(s) 200
Cac8I GCNNGC 5 cut(s) 85, 89, 429, 451, 471
CaiI CAGNNNCTG 1 cut(s) 275
ClaI ATCGAT 1 cut(s) 139
CviAII CATG 1 cut(s) 470
DpnI GATC 3 cut(s) 5, 310, 524
DpnII GATC 3 cut(s) 3, 308, 522
Eam1104I CTCTTC 2 cut(s) 366, 408
EarI CTCTTC 2 cut(s) 366, 408
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 250
EcoICRI GAGCTC 1 cut(s) 250
EcoRII CCWGG 2 cut(s) 95, 126
EcoT38I GRGCYC 1 cut(s) 252
FaeI CATG 1 cut(s) 473
FaiI YATR 2 cut(s) 171, 471
FatI CATG 1 cut(s) 469
Fnu4HI GCNGC 4 cut(s) 228, 231, 332, 395
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 4 cut(s) 228, 231, 332, 395
FspBI CTAG 1 cut(s) 377
GluI GCNGC 4 cut(s) 228, 231, 332, 395
GsaI CCCAGC 1 cut(s) 507
HaeIII GGCC 1 cut(s) 453
Hin1II CATG 1 cut(s) 473
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 1 cut(s) 193
Hpy166II GTNNAC 3 cut(s) 200, 261, 338
Hpy188I TCNGA 1 cut(s) 313
Hpy188III TCNNGA 2 cut(s) 73, 306
Hpy8I GTNNAC 3 cut(s) 200, 261, 338
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4V TGCA 7 cut(s) 51, 91, 157, 233, 269, 278, 431
HpyF10VI GCNNNNNNNGC 4 cut(s) 224, 275, 415, 424
Hsp92II CATG 1 cut(s) 473
Kzo9I GATC 3 cut(s) 3, 308, 522
LguI GCTCTTC 2 cut(s) 366, 408
LmnI GCTCC 2 cut(s) 247, 328
Lsp1109I GCAGC 4 cut(s) 214, 217, 318, 381
MaeI CTAG 1 cut(s) 377
MalI GATC 3 cut(s) 5, 310, 524
MboI GATC 3 cut(s) 3, 308, 522
MboII GAAGA 3 cut(s) 383, 415, 425
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 2 cut(s) 344, 444
MnlI CCTC 5 cut(s) 73, 80, 202, 319, 434
MroXI GAANNNNTTC 1 cut(s) 44
MseI TTAA 2 cut(s) 18, 399
MspA1I CMGCKG 2 cut(s) 14, 394
MspCI CTTAAG 1 cut(s) 398
MspR9I CCNGG 2 cut(s) 97, 128
MvaI CCWGG 2 cut(s) 97, 128
MwoI GCNNNNNNNGC 4 cut(s) 224, 275, 415, 424
NdeII GATC 3 cut(s) 3, 308, 522
NlaIII CATG 1 cut(s) 473
NlaIV GGNNCC 1 cut(s) 28
NspI RCATGY 1 cut(s) 473
PaeI GCATGC 1 cut(s) 473
PciSI GCTCTTC 2 cut(s) 366, 408
PdmI GAANNNNTTC 1 cut(s) 44
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 4 cut(s) 229, 232, 333, 396
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 95, 126
PspFI CCCAGC 1 cut(s) 503
PspGI CCWGG 2 cut(s) 95, 126
PspN4I GGNNCC 1 cut(s) 28
PstI CTGCAG 1 cut(s) 235
PstNI CAGNNNCTG 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 394
SacI GAGCTC 1 cut(s) 252
SapI GCTCTTC 2 cut(s) 366, 408
SaqAI TTAA 2 cut(s) 18, 399
SatI GCNGC 4 cut(s) 228, 231, 332, 395
Sau3AI GATC 3 cut(s) 3, 308, 522
ScrFI CCNGG 2 cut(s) 97, 128
SduI GDGCHC 1 cut(s) 252
SfcI CTRYAG 1 cut(s) 231
SmlI CTYRAG 3 cut(s) 71, 398, 474
SmoI CTYRAG 3 cut(s) 71, 398, 474
SphI GCATGC 1 cut(s) 473
Sse9I AATT 2 cut(s) 344, 444
SsiI CCGC 1 cut(s) 14
SspMI CTAG 1 cut(s) 377
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 95, 126
TaaI ACNGT 1 cut(s) 265
TaqI TCGA 1 cut(s) 139
TasI AATT 2 cut(s) 344, 444
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 2 cut(s) 18, 399
Tru9I TTAA 2 cut(s) 18, 399
TscAI CASTG 1 cut(s) 207
TseI GCWGC 4 cut(s) 227, 230, 331, 394
TspDTI ATGAA 3 cut(s) 100, 371, 384
TspGWI ACGGA 1 cut(s) 178
TspRI CASTG 1 cut(s) 207
Vha464I CTTAAG 1 cut(s) 398
XceI RCATGY 1 cut(s) 473
XmnI GAANNNNTTC 1 cut(s) 44
XspI CTAG 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.