RchiOBHm_Chr5g0009501

OPA3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004304
Physical Location & Seq
Forward (+)
6319665 .. 6322643
2979 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29037

Sequence Viewer

Length: 513 bp
ATGGTACTCCCGCTTGTAAAGCTCGGAACTTTGCTCTTAAAAACTATGAGCAAACCTGTAGCCGCCAGGCTTAAACATGCAGCTGGGAAGCACCCCAGGTTCCGCCAAATCATCGTCAACATGGCTCAGGCAAACCATCAAATCACGACGAAAATGCAAAGACGCCTCTATAGTCACGCAACTGATGTCGAGATTCGTCCCTTGAATGAGGAGAAGGCTGTGCAGGCTGCTGTGGACCTCATCGGGGAGCTCTTTGTCTTCACGGTTGCAGGAGTTGCTCTGATCTTTGAAGTTCAAAGAAGTGCCAGATCAGAAGCCAGAAAGGAGGAAGCACGAAAGCAAGAACTGGAGACAATGAAGCAAAGAGATGAAGAGTTATCTAGAGAAGTGGAGCTTCTAAGGAGCAAATATGAAGAACTGGAGAAGCTTGCTAAGGGACAAGGACTCGGTGGTATTTTTAATTTCAAGCGTGTTGCCGATACAGAAGGTGCAAAGTCAGTAAAACCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPA3

Protein Analysis

170

Amino Acids

19.16

Weight (kDa)

9.8

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPA3 PF07047 6 - 126 5.1e-44 Optic atrophy 3 protein (OPA3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 11, 63, 103
AcyI GRCGYC 1 cut(s) 163
AfaI GTAC 1 cut(s) 6
AfiI CCNNNNNNNGG 1 cut(s) 244
AgsI TTSAA 4 cut(s) 205, 290, 296, 466
AjnI CCWGG 2 cut(s) 65, 95
AluBI AGCT 6 cut(s) 22, 83, 250, 394, 427, 509
AluI AGCT 6 cut(s) 22, 83, 250, 394, 427, 509
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 1 cut(s) 344
ApeKI GCWGC 2 cut(s) 80, 227
AspS9I GGNCC 1 cut(s) 235
AvaII GGWCC 1 cut(s) 235
BanII GRGCYC 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 2 cut(s) 92, 214
BccI CCATC 1 cut(s) 144
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BciT130I CCWGG 2 cut(s) 67, 97
BcoDI GTCTC 1 cut(s) 344
BfaI CTAG 1 cut(s) 381
BfmI CTRYAG 2 cut(s) 57, 169
BisI GCNGC 3 cut(s) 63, 81, 228
BlsI GCNGC 3 cut(s) 64, 82, 229
Bme1390I CCNGG 2 cut(s) 67, 97
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 101
BmrFI CCNGG 2 cut(s) 67, 97
BpiI GAAGAC 1 cut(s) 250
BpmI CTGGAG 2 cut(s) 368, 440
Bpu10I CCTNAGC 2 cut(s) 126, 432
BsaHI GRCGYC 1 cut(s) 163
BsaJI CCNNGG 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 244
Bse1I ACTGG 2 cut(s) 351, 423
BseBI CCWGG 2 cut(s) 67, 97
BseDI CCNNGG 1 cut(s) 95
BseLI CCNNNNNNNGG 1 cut(s) 244
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 2 cut(s) 351, 423
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 2 cut(s) 92, 214
BseYI CCCAGC 1 cut(s) 83
BsgI GTGCAG 1 cut(s) 242
BsiHKAI GWGCWC 1 cut(s) 252
BslFI GGGAC 2 cut(s) 183, 450
BslI CCNNNNNNNGG 1 cut(s) 244
BsmAI GTCTC 1 cut(s) 344
BsmFI GGGAC 2 cut(s) 183, 450
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 2 cut(s) 282, 308
BspACI CCGC 3 cut(s) 11, 63, 103
BspCNI CTCAG 1 cut(s) 139
BspLI GGNNCC 1 cut(s) 101
BsrI ACTGG 2 cut(s) 351, 423
BssECI CCNNGG 1 cut(s) 95
BssMI GATC 2 cut(s) 282, 308
BssNI GRCGYC 1 cut(s) 163
Bst2UI CCWGG 2 cut(s) 67, 97
Bst4CI ACNGT 1 cut(s) 265
Bst6I CTCTTC 1 cut(s) 366
BstACI GRCGYC 1 cut(s) 163
BstAPI GCANNNNNTGC 1 cut(s) 275
BstC8I GCNNGC 2 cut(s) 225, 429
BstDEI CTNAG 3 cut(s) 126, 398, 432
BstKTI GATC 2 cut(s) 285, 311
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 2 cut(s) 282, 308
BstMWI GCNNNNNNNGC 3 cut(s) 19, 224, 275
BstNI CCWGG 2 cut(s) 67, 97
BstNSI RCATGY 1 cut(s) 80
BstSCI CCNGG 2 cut(s) 65, 95
BstSFI CTRYAG 2 cut(s) 57, 169
BstV1I GCAGC 2 cut(s) 92, 214
BstV2I GAAGAC 1 cut(s) 250
Cac8I GCNNGC 2 cut(s) 225, 429
Cfr13I GGNCC 1 cut(s) 235
CseI GACGC 1 cut(s) 171
Csp6I GTAC 1 cut(s) 5
CviAII CATG 2 cut(s) 77, 121
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 3 cut(s) 126, 398, 432
DpnI GATC 2 cut(s) 284, 310
DpnII GATC 2 cut(s) 282, 308
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 250
EcoICRI GAGCTC 1 cut(s) 250
EcoRII CCWGG 2 cut(s) 65, 95
EcoT38I GRGCYC 1 cut(s) 252
FaeI CATG 2 cut(s) 80, 124
FaiI YATR 5 cut(s) 47, 78, 122, 171, 411
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 2 cut(s) 183, 450
FatI CATG 2 cut(s) 76, 120
FauI CCCGC 1 cut(s) 18
Fnu4HI GCNGC 3 cut(s) 63, 81, 228
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 3 cut(s) 63, 81, 228
FspBI CTAG 1 cut(s) 381
GluI GCNGC 3 cut(s) 63, 81, 228
GsaI CCCAGC 1 cut(s) 87
GsuI CTGGAG 2 cut(s) 368, 440
HgaI GACGC 1 cut(s) 171
Hin1I GRCGYC 1 cut(s) 163
Hin1II CATG 2 cut(s) 80, 124
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 2 cut(s) 193, 444
Hpy166II GTNNAC 2 cut(s) 118, 235
Hpy188I TCNGA 3 cut(s) 26, 282, 313
Hpy188III TCNNGA 3 cut(s) 145, 190, 381
Hpy8I GTNNAC 2 cut(s) 118, 235
Hpy99I CGWCG 1 cut(s) 151
HpyAV CCTTC 2 cut(s) 208, 479
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4V TGCA 5 cut(s) 80, 157, 223, 269, 491
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 224, 275
HpyF3I CTNAG 3 cut(s) 126, 398, 432
Hsp92I GRCGYC 1 cut(s) 163
Hsp92II CATG 2 cut(s) 80, 124
Kzo9I GATC 2 cut(s) 282, 308
LmnI GCTCC 3 cut(s) 247, 391, 402
Lsp1109I GCAGC 2 cut(s) 92, 214
MaeI CTAG 1 cut(s) 381
MaeIII GTNAC 1 cut(s) 173
MalI GATC 2 cut(s) 284, 310
MboI GATC 2 cut(s) 282, 308
MboII GAAGA 3 cut(s) 250, 383, 425
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 1 cut(s) 460
MlyI GAGTC 1 cut(s) 438
MnlI CCTC 4 cut(s) 176, 202, 248, 319
MseI TTAA 3 cut(s) 38, 72, 459
MspA1I CMGCKG 1 cut(s) 83
MspR9I CCNGG 2 cut(s) 67, 97
MvaI CCWGG 2 cut(s) 67, 97
MwoI GCNNNNNNNGC 3 cut(s) 19, 224, 275
NdeII GATC 2 cut(s) 282, 308
NlaIII CATG 2 cut(s) 80, 124
NlaIV GGNNCC 1 cut(s) 101
NmuCI GTSAC 1 cut(s) 173
NspI RCATGY 1 cut(s) 80
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 3 cut(s) 64, 82, 229
PleI GAGTC 1 cut(s) 438
PpsI GAGTC 1 cut(s) 438
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 65, 95
PspFI CCCAGC 1 cut(s) 83
PspGI CCWGG 2 cut(s) 65, 95
PspN4I GGNNCC 1 cut(s) 101
PspPI GGNCC 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 3 cut(s) 38, 72, 459
SatI GCNGC 3 cut(s) 63, 81, 228
Sau3AI GATC 2 cut(s) 282, 308
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 1 cut(s) 438
ScrFI CCNGG 2 cut(s) 67, 97
SduI GDGCHC 1 cut(s) 252
SfcI CTRYAG 2 cut(s) 57, 169
SinI GGWCC 1 cut(s) 235
Sse9I AATT 1 cut(s) 460
SsiI CCGC 3 cut(s) 11, 63, 103
SspMI CTAG 1 cut(s) 381
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 65, 95
TaaI ACNGT 1 cut(s) 265
TaqI TCGA 1 cut(s) 189
TasI AATT 1 cut(s) 460
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 3 cut(s) 38, 72, 459
Tru9I TTAA 3 cut(s) 38, 72, 459
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 2 cut(s) 80, 227
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 3 cut(s) 371, 384, 426
VpaK11BI GGWCC 1 cut(s) 235
XbaI TCTAGA 1 cut(s) 380
XceI RCATGY 1 cut(s) 80
XspI CTAG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.