Rh5DG070700

OPA3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004304
Physical Location & Seq
Forward (+)
6093197 .. 6096115
2919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG070700.1

Sequence Viewer

Length: 387 bp
ATGGTACTCCCGCTTGTAAAGCTCGGAACTTTGCTCTTAAAAACAATGAGCAAACCTGTAGCCGCCAGGCTTAAACAGGCAGCTGGGAAGCACCCCAGGTTCCGCCAAATCATCGTCAACATGGCTCAGGCAAACCATCAAATCACGACGAAAATGCAAAGACGCCTCTATAGTCACGCAACTGATGTCGAGATTCGTCCCTTGAATGAGGAGAAGGCTGTGCAGGCTGCTGTGGACCTCATCGGGGAGCTCTTTGTCTTCACGGTTGCAGGAGTTGCTTTGATCTTTGAAGTTCAAAGAAGTGCCAGATCAGAAGCCAGAAAGGAGGAAGCACGAAAGCAAGAACTGGAGGTATGTGCACTAGTTTCTTTGTTAAACAGTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPA3

Protein Analysis

128

Amino Acids

14.29

Weight (kDa)

10.0

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPA3 PF07047 6 - 117 3.8e-42 Optic atrophy 3 protein (OPA3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 11, 63, 103
AcyI GRCGYC 1 cut(s) 163
AfaI GTAC 2 cut(s) 6, 382
AfiI CCNNNNNNNGG 1 cut(s) 244
AgsI TTSAA 3 cut(s) 205, 290, 296
AhlI ACTAGT 1 cut(s) 361
AjnI CCWGG 2 cut(s) 65, 95
AluBI AGCT 3 cut(s) 22, 83, 250
AluI AGCT 3 cut(s) 22, 83, 250
Alw21I GWGCWC 2 cut(s) 252, 361
Alw44I GTGCAC 1 cut(s) 357
ApaLI GTGCAC 1 cut(s) 357
ApeKI GCWGC 2 cut(s) 80, 227
AspS9I GGNCC 1 cut(s) 235
AvaII GGWCC 1 cut(s) 235
BaeGI GKGCMC 1 cut(s) 361
BanII GRGCYC 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 2 cut(s) 252, 361
BbvI GCAGC 2 cut(s) 92, 214
BccI CCATC 1 cut(s) 144
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BciT130I CCWGG 2 cut(s) 67, 97
BcuI ACTAGT 1 cut(s) 361
BfaI CTAG 2 cut(s) 362, 385
BfmI CTRYAG 2 cut(s) 57, 169
BisI GCNGC 3 cut(s) 63, 81, 228
BlsI GCNGC 3 cut(s) 64, 82, 229
Bme1390I CCNGG 2 cut(s) 67, 97
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 101
BmrFI CCNGG 2 cut(s) 67, 97
BpiI GAAGAC 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 368
Bpu10I CCTNAGC 1 cut(s) 126
BsaHI GRCGYC 1 cut(s) 163
BsaJI CCNNGG 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 244
Bse1I ACTGG 1 cut(s) 351
BseBI CCWGG 2 cut(s) 67, 97
BseDI CCNNGG 1 cut(s) 95
BseLI CCNNNNNNNGG 1 cut(s) 244
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 1 cut(s) 351
BseRI GAGGAG 1 cut(s) 224
BseSI GKGCMC 1 cut(s) 361
BseXI GCAGC 2 cut(s) 92, 214
BseYI CCCAGC 1 cut(s) 83
BsgI GTGCAG 1 cut(s) 242
BsiHKAI GWGCWC 2 cut(s) 252, 361
BslFI GGGAC 1 cut(s) 183
BslI CCNNNNNNNGG 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 183
Bsp1286I GDGCHC 2 cut(s) 252, 361
Bsp143I GATC 2 cut(s) 282, 308
BspACI CCGC 3 cut(s) 11, 63, 103
BspCNI CTCAG 1 cut(s) 139
BspLI GGNNCC 1 cut(s) 101
BsrI ACTGG 1 cut(s) 351
BssECI CCNNGG 1 cut(s) 95
BssMI GATC 2 cut(s) 282, 308
BssNI GRCGYC 1 cut(s) 163
Bst2UI CCWGG 2 cut(s) 67, 97
Bst4CI ACNGT 2 cut(s) 265, 380
BstACI GRCGYC 1 cut(s) 163
BstAPI GCANNNNNTGC 1 cut(s) 275
BstC8I GCNNGC 1 cut(s) 225
BstDEI CTNAG 1 cut(s) 126
BstKTI GATC 2 cut(s) 285, 311
BstMBI GATC 2 cut(s) 282, 308
BstMWI GCNNNNNNNGC 3 cut(s) 19, 224, 275
BstNI CCWGG 2 cut(s) 67, 97
BstSCI CCNGG 2 cut(s) 65, 95
BstSFI CTRYAG 2 cut(s) 57, 169
BstSLI GKGCMC 1 cut(s) 361
BstV1I GCAGC 2 cut(s) 92, 214
BstV2I GAAGAC 1 cut(s) 250
Cac8I GCNNGC 1 cut(s) 225
Cfr13I GGNCC 1 cut(s) 235
CseI GACGC 1 cut(s) 171
Csp6I GTAC 2 cut(s) 5, 381
CviAII CATG 1 cut(s) 121
CviJI RGCY 9 cut(s) 22, 62, 70, 83, 125, 218, 227, 250, 317
CviKI_1 RGCY 9 cut(s) 22, 62, 70, 83, 125, 218, 227, 250, 317
CviQI GTAC 2 cut(s) 5, 381
DdeI CTNAG 1 cut(s) 126
DpnI GATC 2 cut(s) 284, 310
DpnII GATC 2 cut(s) 282, 308
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 250
EcoICRI GAGCTC 1 cut(s) 250
EcoRII CCWGG 2 cut(s) 65, 95
EcoT38I GRGCYC 1 cut(s) 252
FaeI CATG 1 cut(s) 124
FaiI YATR 3 cut(s) 122, 171, 355
FaqI GGGAC 1 cut(s) 183
FatI CATG 1 cut(s) 120
FauI CCCGC 1 cut(s) 18
Fnu4HI GCNGC 3 cut(s) 63, 81, 228
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 3 cut(s) 63, 81, 228
FspBI CTAG 2 cut(s) 362, 385
GluI GCNGC 3 cut(s) 63, 81, 228
GsaI CCCAGC 1 cut(s) 87
GsuI CTGGAG 1 cut(s) 368
HgaI GACGC 1 cut(s) 171
Hin1I GRCGYC 1 cut(s) 163
Hin1II CATG 1 cut(s) 124
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HinfI GANTC 1 cut(s) 193
Hpy166II GTNNAC 3 cut(s) 118, 235, 359
Hpy188I TCNGA 2 cut(s) 26, 313
Hpy188III TCNNGA 2 cut(s) 145, 190
Hpy8I GTNNAC 3 cut(s) 118, 235, 359
Hpy99I CGWCG 1 cut(s) 151
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 2 cut(s) 265, 380
HpyCH4V TGCA 4 cut(s) 157, 223, 269, 359
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 224, 275
HpyF3I CTNAG 1 cut(s) 126
Hsp92I GRCGYC 1 cut(s) 163
Hsp92II CATG 1 cut(s) 124
Kzo9I GATC 2 cut(s) 282, 308
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 2 cut(s) 92, 214
MaeI CTAG 2 cut(s) 362, 385
MaeIII GTNAC 1 cut(s) 173
MalI GATC 2 cut(s) 284, 310
MboI GATC 2 cut(s) 282, 308
MboII GAAGA 1 cut(s) 250
MhlI GDGCHC 2 cut(s) 252, 361
MnlI CCTC 5 cut(s) 176, 202, 248, 319, 343
MseI TTAA 3 cut(s) 38, 72, 374
MspA1I CMGCKG 1 cut(s) 83
MspR9I CCNGG 2 cut(s) 67, 97
MvaI CCWGG 2 cut(s) 67, 97
MwoI GCNNNNNNNGC 3 cut(s) 19, 224, 275
NdeII GATC 2 cut(s) 282, 308
NlaIII CATG 1 cut(s) 124
NlaIV GGNNCC 1 cut(s) 101
NmuCI GTSAC 1 cut(s) 173
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 3 cut(s) 64, 82, 229
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 65, 95
PspFI CCCAGC 1 cut(s) 83
PspGI CCWGG 2 cut(s) 65, 95
PspN4I GGNNCC 1 cut(s) 101
PspPI GGNCC 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 2 cut(s) 6, 382
RsaNI GTAC 2 cut(s) 5, 381
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 3 cut(s) 38, 72, 374
SatI GCNGC 3 cut(s) 63, 81, 228
Sau3AI GATC 2 cut(s) 282, 308
Sau96I GGNCC 1 cut(s) 235
ScrFI CCNGG 2 cut(s) 67, 97
SduI GDGCHC 2 cut(s) 252, 361
SetI ASST 8 cut(s) 24, 58, 85, 101, 240, 252, 354, 386
SfcI CTRYAG 2 cut(s) 57, 169
SinI GGWCC 1 cut(s) 235
SpeI ACTAGT 1 cut(s) 361
SsiI CCGC 3 cut(s) 11, 63, 103
SspMI CTAG 2 cut(s) 362, 385
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 65, 95
TaaI ACNGT 2 cut(s) 265, 380
TaqI TCGA 1 cut(s) 189
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 3 cut(s) 38, 72, 374
Tru9I TTAA 3 cut(s) 38, 72, 374
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 2 cut(s) 80, 227
Tsp45I GTSAC 1 cut(s) 173
VneI GTGCAC 1 cut(s) 357
VpaK11BI GGWCC 1 cut(s) 235
XspI CTAG 2 cut(s) 362, 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.