MD10G1287400.v1.1

OPA3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004304
Physical Location & Seq
Reverse (-)
37669148 .. 37672142
2995 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1287400.v1.1.491

Sequence Viewer

Length: 510 bp
ATGGTGCTTCCGCTCCTGAAGCTCGGGACTTTGGCCATCAAAACTCTGAGCAAACCCGTCGCCGCCAGGCTCAAACATCAAGCTGCAATGCACCCCAAGTTCCGCCAGTTCATCGTCAGCATCGCACAGGCAAACCATCGAATGACCACGAGAATGCAAAGGCGCATATATAGTCGTGCCACTGATGTTAAGATTCGCCCTTTGAATGAGGAGAAGGCTGTGCAGGCTGCTGTGGACCTTATTGGCGAGCTCTTTGTCTTCTCGGTTGCAACAGTTGCTTTGATATTTGAAGTGCAAAGAAGTGCTCGATCAGAAGCCAGAAAGGAGGAAGCACGCAAGCGAGAACTGGAGGCACTGAAGCAAAGAGACGAAGCCTTATCAAGAGAAGTAGAACTTCTTAAGGAAAAACTTGAAGAACTAGAGAAGCTTGCCAGGGGACGTGGACTTAGTGGGATTATCAGTTTCAAGCGCCCTGATACAGAATCTGCAAAGTCAGTAAAGCCAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OPA3

Protein Analysis

170

Amino Acids

19.14

Weight (kDa)

10.33

Isoelectric Point (pI)

32.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OPA3 PF07047 6 - 126 2e-43 Optic atrophy 3 protein (OPA3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 13
AciI CCGC 3 cut(s) 11, 63, 103
AcoI YGGCCR 1 cut(s) 33
AcuI CTGAAG 2 cut(s) 38, 377
AflII CTTAAG 1 cut(s) 398
AgsI TTSAA 4 cut(s) 205, 290, 413, 466
AjiI CACGTC 1 cut(s) 440
AjnI CCWGG 2 cut(s) 65, 431
AluBI AGCT 4 cut(s) 22, 83, 250, 427
AluI AGCT 4 cut(s) 22, 83, 250, 427
Alw21I GWGCWC 2 cut(s) 252, 307
Alw26I GTCTC 1 cut(s) 360
AlwNI CAGNNNCTG 1 cut(s) 485
Ama87I CYCGRG 1 cut(s) 23
AoxI GGCC 1 cut(s) 33
ApeKI GCWGC 2 cut(s) 83, 227
AspLEI GCGC 2 cut(s) 165, 471
AspS9I GGNCC 1 cut(s) 235
AvaI CYCGRG 1 cut(s) 23
AvaII GGWCC 1 cut(s) 235
BalI TGGCCA 1 cut(s) 35
BanII GRGCYC 1 cut(s) 252
BauI CACGAG 1 cut(s) 148
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 2 cut(s) 252, 307
BbvI GCAGC 2 cut(s) 70, 214
BccI CCATC 2 cut(s) 44, 144
BcgI CGANNNNNNTGC 2 cut(s) 40, 74
BciT130I CCWGG 2 cut(s) 67, 433
BcoDI GTCTC 1 cut(s) 360
BfaI CTAG 1 cut(s) 419
BfoI RGCGCY 1 cut(s) 472
BfrI CTTAAG 1 cut(s) 398
BisI GCNGC 3 cut(s) 63, 84, 228
BlsI GCNGC 3 cut(s) 64, 85, 229
Bme1390I CCNGG 2 cut(s) 67, 433
Bme18I GGWCC 1 cut(s) 235
BmeT110I CYCGRG 1 cut(s) 23
BmgBI CACGTC 1 cut(s) 440
BmgT120I GGNCC 1 cut(s) 235
BmrFI CCNGG 2 cut(s) 67, 433
BmsI GCATC 1 cut(s) 129
BpiI GAAGAC 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 368
BsaJI CCNNGG 1 cut(s) 432
Bse1I ACTGG 2 cut(s) 106, 351
Bse3DI GCAATG 1 cut(s) 93
BseBI CCWGG 2 cut(s) 67, 433
BseDI CCNNGG 1 cut(s) 432
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 2 cut(s) 106, 351
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 2 cut(s) 70, 214
BsgI GTGCAG 1 cut(s) 242
BshFI GGCC 1 cut(s) 35
BsiHKAI GWGCWC 2 cut(s) 252, 307
BsiHKCI CYCGRG 1 cut(s) 23
BslFI GGGAC 2 cut(s) 40, 450
BsmAI GTCTC 1 cut(s) 360
BsmBI CGTCTC 1 cut(s) 360
BsmFI GGGAC 2 cut(s) 40, 450
BsmI GAATGC 1 cut(s) 159
BsnI GGCC 1 cut(s) 35
BsoBI CYCGRG 1 cut(s) 23
Bsp1286I GDGCHC 2 cut(s) 252, 307
Bsp143I GATC 1 cut(s) 308
BspACI CCGC 3 cut(s) 11, 63, 103
BspANI GGCC 1 cut(s) 35
BspCNI CTCAG 1 cut(s) 39
BspTI CTTAAG 1 cut(s) 398
BsrBI CCGCTC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 93
BsrI ACTGG 2 cut(s) 106, 351
BssECI CCNNGG 1 cut(s) 432
BssMI GATC 1 cut(s) 308
BssSI CACGAG 1 cut(s) 148
Bst2BI CACGAG 1 cut(s) 148
Bst2UI CCWGG 2 cut(s) 67, 433
Bst4CI ACNGT 1 cut(s) 274
BstAFI CTTAAG 1 cut(s) 398
BstAPI GCANNNNNTGC 1 cut(s) 275
BstC8I GCNNGC 6 cut(s) 225, 248, 334, 338, 429, 504
BstDEI CTNAG 2 cut(s) 47, 446
BstH2I RGCGCY 1 cut(s) 472
BstHHI GCGC 2 cut(s) 165, 471
BstKTI GATC 1 cut(s) 311
BstMAI GTCTC 1 cut(s) 360
BstMBI GATC 1 cut(s) 308
BstMWI GCNNNNNNNGC 3 cut(s) 19, 224, 275
BstNI CCWGG 2 cut(s) 67, 433
BstSCI CCNGG 2 cut(s) 65, 431
BstV1I GCAGC 2 cut(s) 70, 214
BstV2I GAAGAC 1 cut(s) 250
BsuRI GGCC 1 cut(s) 35
BtgZI GCGATG 1 cut(s) 106
BtrI CACGTC 1 cut(s) 440
BtsIMutI CAGTG 2 cut(s) 180, 353
Cac8I GCNNGC 6 cut(s) 225, 248, 334, 338, 429, 504
CaiI CAGNNNCTG 1 cut(s) 485
CfoI GCGC 2 cut(s) 165, 471
Cfr13I GGNCC 1 cut(s) 235
DdeI CTNAG 2 cut(s) 47, 446
DpnI GATC 1 cut(s) 310
DpnII GATC 1 cut(s) 308
EaeI YGGCCR 1 cut(s) 33
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 2 cut(s) 38, 377
Eco88I CYCGRG 1 cut(s) 23
EcoICRI GAGCTC 1 cut(s) 250
EcoRII CCWGG 2 cut(s) 65, 431
EcoT38I GRGCYC 1 cut(s) 252
Esp3I CGTCTC 1 cut(s) 360
FaiI YATR 3 cut(s) 167, 169, 171
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 2 cut(s) 40, 450
Fnu4HI GCNGC 3 cut(s) 63, 84, 228
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 3 cut(s) 63, 84, 228
FspBI CTAG 1 cut(s) 419
GlaI GCGC 2 cut(s) 164, 470
GluI GCNGC 3 cut(s) 63, 84, 228
GsuI CTGGAG 1 cut(s) 368
HaeII RGCGCY 1 cut(s) 472
HaeIII GGCC 1 cut(s) 35
HhaI GCGC 2 cut(s) 165, 471
Hin6I GCGC 2 cut(s) 163, 469
HinP1I GCGC 2 cut(s) 163, 469
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 2 cut(s) 193, 482
Hpy166II GTNNAC 2 cut(s) 235, 443
Hpy188I TCNGA 2 cut(s) 48, 313
Hpy188III TCNNGA 3 cut(s) 16, 25, 381
Hpy8I GTNNAC 2 cut(s) 235, 443
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 7 cut(s) 86, 91, 157, 223, 269, 295, 488
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 224, 275
HpyF3I CTNAG 2 cut(s) 47, 446
HpySE526I ACGT 1 cut(s) 439
HspAI GCGC 2 cut(s) 163, 469
Kzo9I GATC 1 cut(s) 308
LmnI GCTCC 1 cut(s) 18
Lsp1109I GCAGC 2 cut(s) 70, 214
LweI GCATC 1 cut(s) 129
MaeI CTAG 1 cut(s) 419
MaeII ACGT 1 cut(s) 439
MalI GATC 1 cut(s) 310
MbiI CCGCTC 1 cut(s) 13
MboI GATC 1 cut(s) 308
MboII GAAGA 2 cut(s) 250, 425
MhlI GDGCHC 2 cut(s) 252, 307
MlsI TGGCCA 1 cut(s) 35
MluNI TGGCCA 1 cut(s) 35
MnlI CCTC 3 cut(s) 202, 319, 343
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
MseI TTAA 2 cut(s) 189, 399
MslI CAYNNNNRTG 1 cut(s) 152
Msp20I TGGCCA 1 cut(s) 35
MspCI CTTAAG 1 cut(s) 398
MspR9I CCNGG 2 cut(s) 67, 433
Mva1269I GAATGC 1 cut(s) 159
MvaI CCWGG 2 cut(s) 67, 433
MwoI GCNNNNNNNGC 3 cut(s) 19, 224, 275
NdeII GATC 1 cut(s) 308
PctI GAATGC 1 cut(s) 159
PfeI GAWTC 2 cut(s) 193, 482
PkrI GCNGC 3 cut(s) 64, 85, 229
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 65, 431
PspGI CCWGG 2 cut(s) 65, 431
PspPI GGNCC 1 cut(s) 235
PstNI CAGNNNCTG 1 cut(s) 485
RseI CAYNNNNRTG 1 cut(s) 152
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 2 cut(s) 189, 399
SatI GCNGC 3 cut(s) 63, 84, 228
Sau3AI GATC 1 cut(s) 308
Sau96I GGNCC 1 cut(s) 235
ScrFI CCNGG 2 cut(s) 67, 433
SduI GDGCHC 2 cut(s) 252, 307
SetI ASST 6 cut(s) 24, 85, 240, 252, 429, 442
SfaNI GCATC 1 cut(s) 129
SinI GGWCC 1 cut(s) 235
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
SsiI CCGC 3 cut(s) 11, 63, 103
SspMI CTAG 1 cut(s) 419
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 65, 431
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 1 cut(s) 442
TaqI TCGA 2 cut(s) 139, 307
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 2 cut(s) 193, 482
Tru1I TTAA 2 cut(s) 189, 399
Tru9I TTAA 2 cut(s) 189, 399
TscAI CASTG 2 cut(s) 187, 360
TseI GCWGC 2 cut(s) 83, 227
TspDTI ATGAA 1 cut(s) 100
TspRI CASTG 2 cut(s) 187, 360
Vha464I CTTAAG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 235
XspI CTAG 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.