FvH4_3g22630

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005748
Physical Location & Seq
Reverse (-)
15664324 .. 15667491
3168 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22630.t1

Sequence Viewer

Length: 1476 bp
ATGGCCGCCCACTACGAACCTCCTTCTTTCTCTCTCGGACTCGATTTCGGGTTTGAATCGGAGCTCCAAACAGCTCCTCCGCCCAACTCCACTCCTCCGCGTCCGGTTGACACTGATCTCGATCAAGAGCCCGAGCCACAGATCGAACCGCCGCGGGCGCTCAAGCGGCTGAAGCGAGGCCTGCCGGAGAAGCGGGAGCTCCCGACGACGCCGTTTTGGAGTGTTGAAGACGACATCGAAGAGTTCTCTTCTCCCGAAGATACTGTTCAAGCAGATGTGCATCGGTCAACTCAATACCAAACATCTTGTATTAGTTCCAAAGTCCCATTGCCTGGAAGTGAGGTTTTGACTTCTCAATCATCCTACCATAGCATTGGAAGGAAAAAGAATTACAGTTCAGATGTGCCAGCTTCTGTGAGTTTGGAGGCAAGCCGCAAAGAATTTGTTTTTCCAAAGTTAACTACCAGTCCTCTTCGTCGGTTTCAGTTGATTGATTCCGATTCTGATGATTATCCAGTCAGTCATAGTGTTAGTAGGCTTAATTCTGAGGTTGATTCATCCTCAAAGAAGCAACAAAGTAACCCTGGCCATTATGCAACTACTAGTGGAACAAACAAAAACACATCTACCATTATGCCTGAAGATGTTGATTTGTGGAAAAACTTTAGTCCAATCAAGAAGTTTAACATTCCAACACCTGCTTTGGATGAGGCATGTCAAGAGTACTTCCAATCCGTGAAGGATAAGACTACTCAAAATCTTGAGAGGGATGCATATGTGCATACCAATGAGAATTTTCATGATACGACACCCGGTGGCCAAGATTTTGAATATCTCTGGAATGAGGCTGATCCCCTTCTGCCTGCCCATCATTATTTTTTCCATGATGATCCAAAGATTCGAAAGTTAGTCTGCAGTCGCTTGCCAAATTTTTTCCCCTTGGGCGCTGTTAGTGTCGGTGGAAATCAACAGAATGGTGCATCAGTTATTGATTACATAGGTCAATTTAGCAATGGAGAAGCTTCAAAACAGACGGTGAATCGGAGACAAAATGTTTCAAACATATCTAATGTTGAAGAATTCGTGCATGGATCTGGAAACTGGGTGAACCCAAAAGGTAAAGCGACACGGAAAGCTAGTGTCAAGCGGAGTGTAAACAAGGGAAGCAATAAGTCAGTCAAATCAAACACTGGGAACAAGGAGCATGCTGCTGGAAACTGGGTGGAGCCCAGAATCTCAGCCAGCAAAAAGCGGATTCAAGCCAATGCACAATCTTCTGGTGAATGGCACACACCTAGTCAAAGTAGCCAAGCTAGTAGGCAAGCTGGTGGTCATTGGTATACAGGCTCTGGTGGAAGGAAGGTTTATGTCAGTAAAACTGGGAAGGAATTGACTGGTCGAACAGCCTACAATGCGTATACAAAGGAAAGTGGGAAGCGGTCTGCGAAGCCCAGAAAAGGGAAAGCCAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

492

Amino Acids

54.48

Weight (kDa)

9.05

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 706
Acc36I ACCTGC 1 cut(s) 706
AccB7I CCANNNNNTGG 1 cut(s) 332
AccI GTMKAC 2 cut(s) 1340, 1418
AccII CGCG 2 cut(s) 100, 154
AclWI GGATC 3 cut(s) 845, 884, 1099
AcoI YGGCCR 3 cut(s) 3, 586, 817
AcsI RAATTY 4 cut(s) 440, 793, 928, 1079
AcuI CTGAAG 2 cut(s) 191, 660
AcyI GRCGYC 1 cut(s) 209
AdeI CACNNNGTG 1 cut(s) 815
AfaI GTAC 1 cut(s) 725
AfiI CCNNNNNNNGG 3 cut(s) 332, 1457, 1458
AgsI TTSAA 8 cut(s) 56, 227, 269, 830, 1026, 1059, 1076, 1259
AhlI ACTAGT 1 cut(s) 602
AjnI CCWGG 2 cut(s) 331, 583
AluBI AGCT 8 cut(s) 64, 74, 199, 410, 1022, 1136, 1313, 1325
AluI AGCT 8 cut(s) 64, 74, 199, 410, 1022, 1136, 1313, 1325
Alw21I GWGCWC 2 cut(s) 66, 201
Alw26I GTCTC 1 cut(s) 1039
AlwI GGATC 3 cut(s) 845, 884, 1099
AlwNI CAGNNNCTG 2 cut(s) 413, 1349
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 4 cut(s) 3, 178, 586, 817
ApeKI GCWGC 1 cut(s) 1208
ApoI RAATTY 4 cut(s) 440, 793, 928, 1079
AspLEI GCGC 2 cut(s) 160, 947
AsuC2I CCSGG 1 cut(s) 813
AsuHPI GGTGA 3 cut(s) 1048, 1117, 1292
AsuII TTCGAA 1 cut(s) 901
AvaI CYCGRG 1 cut(s) 131
BalI TGGCCA 2 cut(s) 588, 819
BanII GRGCYC 4 cut(s) 66, 132, 201, 1230
BbsI GAAGAC 1 cut(s) 234
Bbv12I GWGCWC 2 cut(s) 66, 201
BbvI GCAGC 1 cut(s) 1195
BccI CCATC 1 cut(s) 876
BceAI ACGGC 1 cut(s) 196
BciT130I CCWGG 2 cut(s) 333, 585
BcnI CCSGG 1 cut(s) 813
BcoDI GTCTC 1 cut(s) 1039
BcuI ACTAGT 1 cut(s) 602
BfaI CTAG 4 cut(s) 603, 1137, 1296, 1314
BfmI CTRYAG 1 cut(s) 913
BfoI RGCGCY 2 cut(s) 161, 948
BfuAI ACCTGC 1 cut(s) 706
BisI GCNGC 5 cut(s) 6, 152, 167, 433, 1209
BlsI GCNGC 5 cut(s) 7, 153, 168, 434, 1210
BmcAI AGTACT 1 cut(s) 725
Bme1390I CCNGG 3 cut(s) 333, 585, 813
BmeT110I CYCGRG 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 1227
BmrFI CCNGG 3 cut(s) 333, 585, 813
BmrI ACTGGG 4 cut(s) 1111, 1200, 1228, 1389
BmsI GCATC 3 cut(s) 289, 760, 989
BmuI ACTGGG 4 cut(s) 1111, 1200, 1228, 1389
BpiI GAAGAC 1 cut(s) 234
Bpu14I TTCGAA 1 cut(s) 901
BpuEI CTTGAG 2 cut(s) 146, 782
BpuMI CCSGG 1 cut(s) 813
BsaBI GATNNNNATC 3 cut(s) 120, 279, 510
BsaHI GRCGYC 1 cut(s) 209
BsaJI CCNNGG 3 cut(s) 152, 583, 939
BsaWI WCCGGW 1 cut(s) 103
BsaXI ACNNNNNCTCC 2 cut(s) 61, 91
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 1457, 1458
Bse1I ACTGG 7 cut(s) 465, 515, 1106, 1195, 1223, 1384, 1399
Bse3DI GCAATG 2 cut(s) 326, 1018
Bse8I GATNNNNATC 3 cut(s) 120, 279, 510
BseBI CCWGG 2 cut(s) 333, 585
BseDI CCNNGG 3 cut(s) 152, 583, 939
BseGI GGATG 4 cut(s) 359, 557, 712, 775
BseJI GATNNNNATC 3 cut(s) 120, 279, 510
BseLI CCNNNNNNNGG 3 cut(s) 332, 1457, 1458
BseMI GCAATG 2 cut(s) 326, 1018
BseMII CTCAG 2 cut(s) 537, 1251
BseNI ACTGG 7 cut(s) 465, 515, 1106, 1195, 1223, 1384, 1399
BseRI GAGGAG 2 cut(s) 66, 84
BseXI GCAGC 1 cut(s) 1195
Bsh1236I CGCG 2 cut(s) 100, 154
BshFI GGCC 4 cut(s) 5, 180, 588, 819
BsiHKAI GWGCWC 2 cut(s) 66, 201
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 3 cut(s) 104, 185, 813
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 3 cut(s) 332, 1457, 1458
BsmAI GTCTC 1 cut(s) 1039
BsmFI GGGAC 1 cut(s) 308
BsnI GGCC 4 cut(s) 5, 180, 588, 819
BsoBI CYCGRG 1 cut(s) 131
Bsp119I TTCGAA 1 cut(s) 901
Bsp1286I GDGCHC 4 cut(s) 66, 132, 201, 1230
Bsp143I GATC 6 cut(s) 115, 121, 141, 850, 889, 1091
BspANI GGCC 4 cut(s) 5, 180, 588, 819
BspCNI CTCAG 2 cut(s) 538, 1250
BspFNI CGCG 2 cut(s) 100, 154
BspHI TCATGA 1 cut(s) 799
BspLI GGNNCC 1 cut(s) 1227
BspMAI CTGCAG 1 cut(s) 917
BspMI ACCTGC 1 cut(s) 706
BspPI GGATC 3 cut(s) 845, 884, 1099
BspT104I TTCGAA 1 cut(s) 901
BsrDI GCAATG 2 cut(s) 326, 1018
BsrI ACTGG 7 cut(s) 465, 515, 1106, 1195, 1223, 1384, 1399
BssECI CCNNGG 3 cut(s) 152, 583, 939
BssMI GATC 6 cut(s) 115, 121, 141, 850, 889, 1091
BssNAI GTATAC 2 cut(s) 1341, 1419
BssNI GRCGYC 1 cut(s) 209
BssT1I CCWWGG 1 cut(s) 939
Bst1107I GTATAC 2 cut(s) 1341, 1419
Bst2UI CCWGG 2 cut(s) 333, 585
Bst4CI ACNGT 3 cut(s) 265, 395, 1036
Bst6I CTCTTC 3 cut(s) 234, 253, 477
BstACI GRCGYC 1 cut(s) 209
BstBI TTCGAA 1 cut(s) 901
BstC8I GCNNGC 9 cut(s) 156, 182, 408, 430, 864, 923, 1206, 1243, 1323
BstDEI CTNAG 2 cut(s) 546, 1237
BstDSI CCRYGG 1 cut(s) 152
BstF5I GGATG 4 cut(s) 359, 557, 712, 775
BstFNI CGCG 2 cut(s) 100, 154
BstH2I RGCGCY 2 cut(s) 161, 948
BstHHI GCGC 2 cut(s) 160, 947
BstKTI GATC 6 cut(s) 118, 124, 144, 853, 892, 1094
BstMAI GTCTC 1 cut(s) 1039
BstMBI GATC 6 cut(s) 115, 121, 141, 850, 889, 1091
BstMWI GCNNNNNNNGC 6 cut(s) 157, 166, 172, 181, 190, 1412
BstNI CCWGG 2 cut(s) 333, 585
BstNSI RCATGY 2 cut(s) 717, 1208
BstSCI CCNGG 3 cut(s) 331, 583, 811
BstSFI CTRYAG 1 cut(s) 913
BstUI CGCG 2 cut(s) 100, 154
BstV1I GCAGC 1 cut(s) 1195
BstV2I GAAGAC 1 cut(s) 234
BstX2I RGATCY 1 cut(s) 1091
BstXI CCANNNNNNTGG 1 cut(s) 374
BstYI RGATCY 1 cut(s) 1091
BstZ17I GTATAC 2 cut(s) 1341, 1419
BsuRI GGCC 4 cut(s) 5, 180, 588, 819
BtgI CCRYGG 1 cut(s) 152
BtsCI GGATG 4 cut(s) 359, 557, 712, 775
BtsIMutI CAGTG 2 cut(s) 111, 1188
BveI ACCTGC 1 cut(s) 706
Cac8I GCNNGC 9 cut(s) 156, 182, 408, 430, 864, 923, 1206, 1243, 1323
CaiI CAGNNNCTG 2 cut(s) 413, 1349
CciI TCATGA 1 cut(s) 799
CfoI GCGC 2 cut(s) 160, 947
Cfr42I CCGCGG 1 cut(s) 155
CseI GACGC 2 cut(s) 89, 217
Csp6I GTAC 1 cut(s) 724
CviAII CATG 5 cut(s) 714, 800, 884, 1088, 1205
CviQI GTAC 1 cut(s) 724
DdeI CTNAG 2 cut(s) 546, 1237
DpnI GATC 6 cut(s) 117, 123, 143, 852, 891, 1093
DpnII GATC 6 cut(s) 115, 121, 141, 850, 889, 1091
DraIII CACNNNGTG 1 cut(s) 815
EaeI YGGCCR 3 cut(s) 3, 586, 817
Eam1104I CTCTTC 3 cut(s) 234, 253, 477
EarI CTCTTC 3 cut(s) 234, 253, 477
EciI GGCGGA 1 cut(s) 69
Ecl136II GAGCTC 2 cut(s) 64, 199
Eco130I CCWWGG 1 cut(s) 939
Eco147I AGGCCT 1 cut(s) 180
Eco24I GRGCYC 4 cut(s) 66, 132, 201, 1230
Eco53kI GAGCTC 2 cut(s) 64, 199
Eco57I CTGAAG 2 cut(s) 191, 660
Eco88I CYCGRG 1 cut(s) 131
EcoICRI GAGCTC 2 cut(s) 64, 199
EcoRI GAATTC 1 cut(s) 1079
EcoRII CCWGG 2 cut(s) 331, 583
EcoT14I CCWWGG 1 cut(s) 939
EcoT22I ATGCAT 1 cut(s) 775
EcoT38I GRGCYC 4 cut(s) 66, 132, 201, 1230
ErhI CCWWGG 1 cut(s) 939
FaeI CATG 5 cut(s) 717, 803, 887, 1091, 1208
FaqI GGGAC 1 cut(s) 308
FatI CATG 5 cut(s) 713, 799, 883, 1087, 1204
FauI CCCGC 2 cut(s) 147, 186
FauNDI CATATG 1 cut(s) 775
FblI GTMKAC 2 cut(s) 1340, 1418
Fnu4HI GCNGC 5 cut(s) 6, 152, 167, 433, 1209
FokI GGATG 4 cut(s) 346, 544, 719, 782
FriOI GRGCYC 4 cut(s) 66, 132, 201, 1230
Fsp4HI GCNGC 5 cut(s) 6, 152, 167, 433, 1209
FspBI CTAG 4 cut(s) 603, 1137, 1296, 1314
GlaI GCGC 2 cut(s) 159, 946
GluI GCNGC 5 cut(s) 6, 152, 167, 433, 1209
HaeII RGCGCY 2 cut(s) 161, 948
HaeIII GGCC 4 cut(s) 5, 180, 588, 819
HapII CCGG 3 cut(s) 104, 185, 813
HgaI GACGC 2 cut(s) 89, 217
HhaI GCGC 2 cut(s) 160, 947
Hin1I GRCGYC 1 cut(s) 209
Hin1II CATG 5 cut(s) 717, 803, 887, 1091, 1208
Hin6I GCGC 2 cut(s) 158, 945
HinP1I GCGC 2 cut(s) 158, 945
HincII GTYRAC 3 cut(s) 109, 288, 459
HindII GTYRAC 3 cut(s) 109, 288, 459
HindIII AAGCTT 1 cut(s) 1020
HinfI GANTC 9 cut(s) 39, 56, 494, 500, 554, 898, 1039, 1233, 1255
HpaI GTTAAC 1 cut(s) 459
HpaII CCGG 3 cut(s) 104, 185, 813
HphI GGTGA 3 cut(s) 1048, 1117, 1292
Hpy166II GTNNAC 7 cut(s) 109, 288, 459, 1108, 1156, 1341, 1419
Hpy188I TCNGA 7 cut(s) 38, 61, 400, 499, 505, 547, 1044
Hpy8I GTNNAC 7 cut(s) 109, 288, 459, 1108, 1156, 1341, 1419
Hpy99I CGWCG 3 cut(s) 208, 211, 480
HpyAV CCTTC 7 cut(s) 33, 372, 733, 866, 1350, 1354, 1378
HpyCH4III ACNGT 3 cut(s) 265, 395, 1036
HpyCH4V TGCA 8 cut(s) 280, 596, 773, 781, 915, 980, 1087, 1268
HpyF10VI GCNNNNNNNGC 6 cut(s) 157, 166, 172, 181, 190, 1412
HpyF3I CTNAG 2 cut(s) 546, 1237
Hsp92I GRCGYC 1 cut(s) 209
Hsp92II CATG 5 cut(s) 717, 803, 887, 1091, 1208
HspAI GCGC 2 cut(s) 158, 945
KspAI GTTAAC 1 cut(s) 459
KspI CCGCGG 1 cut(s) 155
Kzo9I GATC 6 cut(s) 115, 121, 141, 850, 889, 1091
LmnI GCTCC 7 cut(s) 61, 69, 79, 196, 204, 1201, 1225
Lsp1109I GCAGC 1 cut(s) 1195
LweI GCATC 3 cut(s) 289, 760, 989
MaeI CTAG 4 cut(s) 603, 1137, 1296, 1314
MaeIII GTNAC 1 cut(s) 578
MalI GATC 6 cut(s) 117, 123, 143, 852, 891, 1093
MboI GATC 6 cut(s) 115, 121, 141, 850, 889, 1091
MboII GAAGA 8 cut(s) 239, 240, 251, 269, 464, 653, 1088, 1266
MflI RGATCY 1 cut(s) 1091
MhlI GDGCHC 4 cut(s) 66, 132, 201, 1230
MlsI TGGCCA 2 cut(s) 588, 819
MluCI AATT 8 cut(s) 388, 440, 541, 793, 928, 1004, 1079, 1388
MluNI TGGCCA 2 cut(s) 588, 819
MlyI GAGTC 1 cut(s) 33
MmeI TCCRAC 1 cut(s) 716
Mox20I TGGCCA 2 cut(s) 588, 819
Mph1103I ATGCAT 1 cut(s) 775
MscI TGGCCA 2 cut(s) 588, 819
MseI TTAA 3 cut(s) 458, 540, 684
MslI CAYNNNNRTG 1 cut(s) 786
Msp20I TGGCCA 2 cut(s) 588, 819
MspA1I CMGCKG 1 cut(s) 154
MspI CCGG 3 cut(s) 104, 185, 813
MspR9I CCNGG 3 cut(s) 333, 585, 813
MvaI CCWGG 2 cut(s) 333, 585
MvnI CGCG 2 cut(s) 100, 154
MwoI GCNNNNNNNGC 6 cut(s) 157, 166, 172, 181, 190, 1412
NciI CCSGG 1 cut(s) 813
NdeI CATATG 1 cut(s) 775
NdeII GATC 6 cut(s) 115, 121, 141, 850, 889, 1091
NlaIII CATG 5 cut(s) 717, 803, 887, 1091, 1208
NlaIV GGNNCC 1 cut(s) 1227
NsiI ATGCAT 1 cut(s) 775
NspI RCATGY 2 cut(s) 717, 1208
NspV TTCGAA 1 cut(s) 901
PaeI GCATGC 1 cut(s) 1208
PagI TCATGA 1 cut(s) 799
PaqCI CACCTGC 1 cut(s) 706
PceI AGGCCT 1 cut(s) 180
PfeI GAWTC 8 cut(s) 56, 494, 500, 554, 898, 1039, 1233, 1255
PflMI CCANNNNNTGG 1 cut(s) 332
PkrI GCNGC 5 cut(s) 7, 153, 168, 434, 1210
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
Psp124BI GAGCTC 2 cut(s) 66, 201
Psp6I CCWGG 2 cut(s) 331, 583
PspGI CCWGG 2 cut(s) 331, 583
PspN4I GGNNCC 1 cut(s) 1227
PstI CTGCAG 1 cut(s) 917
PstNI CAGNNNCTG 2 cut(s) 413, 1349
PsuI RGATCY 1 cut(s) 1091
RsaI GTAC 1 cut(s) 725
RsaNI GTAC 1 cut(s) 724
RseI CAYNNNNRTG 1 cut(s) 786
SacI GAGCTC 2 cut(s) 66, 201
SacII CCGCGG 1 cut(s) 155
SaqAI TTAA 3 cut(s) 458, 540, 684
SatI GCNGC 5 cut(s) 6, 152, 167, 433, 1209
Sau3AI GATC 6 cut(s) 115, 121, 141, 850, 889, 1091
ScaI AGTACT 1 cut(s) 725
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 3 cut(s) 333, 585, 813
SduI GDGCHC 4 cut(s) 66, 132, 201, 1230
SfaNI GCATC 3 cut(s) 289, 760, 989
SfcI CTRYAG 1 cut(s) 913
Sfr303I CCGCGG 1 cut(s) 155
SfuI TTCGAA 1 cut(s) 901
SgrBI CCGCGG 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 786
SmlI CTYRAG 2 cut(s) 161, 761
SmoI CTYRAG 2 cut(s) 161, 761
SpeI ACTAGT 1 cut(s) 602
SphI GCATGC 1 cut(s) 1208
Sse9I AATT 8 cut(s) 388, 440, 541, 793, 928, 1004, 1079, 1388
SseBI AGGCCT 1 cut(s) 180
SspMI CTAG 4 cut(s) 603, 1137, 1296, 1314
SstI GAGCTC 2 cut(s) 66, 201
StuI AGGCCT 1 cut(s) 180
StyD4I CCNGG 3 cut(s) 331, 583, 811
StyI CCWWGG 1 cut(s) 939
TaaI ACNGT 3 cut(s) 265, 395, 1036
TaqI TCGA 6 cut(s) 42, 120, 144, 237, 901, 1399
TaqII GACCGA 1 cut(s) 273
TasI AATT 8 cut(s) 388, 440, 541, 793, 928, 1004, 1079, 1388
TatI WGTACW 1 cut(s) 723
TauI GCSGC 4 cut(s) 8, 154, 169, 435
TfiI GAWTC 8 cut(s) 56, 494, 500, 554, 898, 1039, 1233, 1255
Tru1I TTAA 3 cut(s) 458, 540, 684
Tru9I TTAA 3 cut(s) 458, 540, 684
TscAI CASTG 2 cut(s) 118, 1195
TseI GCWGC 1 cut(s) 1208
TspDTI ATGAA 2 cut(s) 546, 788
TspGWI ACGGA 2 cut(s) 724, 1144
TspRI CASTG 2 cut(s) 118, 1195
Van91I CCANNNNNTGG 1 cut(s) 332
XapI RAATTY 4 cut(s) 440, 793, 928, 1079
XceI RCATGY 2 cut(s) 717, 1208
XmiI GTMKAC 2 cut(s) 1340, 1418
XspI CTAG 4 cut(s) 603, 1137, 1296, 1314
ZrmI AGTACT 1 cut(s) 725
Zsp2I ATGCAT 1 cut(s) 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.