Rh5DG278000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005748
Physical Location & Seq
Reverse (-)
34006120 .. 34009346
3227 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG278000.1

Sequence Viewer

Length: 1536 bp
ATGGCCGCCGACTACGAACCTCCTTCTTTCTCTCTCGGACTCGATTTCGGGTTCGAATCGGAGCTCCAAACAGCTCCTCCGCCCGACTCCAGTCCTCCGCGTCCTGTTGACACTGATGTGGATCGAGAGATCGAGCCGCCGCGGGAGCTCAAGAGGCTTAAGCGAGGTCTGCCGGAGAAGCGGGAGCCCCCGACGACGCCGATTTGGAATATTGAAGATGACATCGAAGAGTTCTCTTCTCCCGAAGATACTATTTGTGCAGATGTGCAACGGTCAACACAGTACCAAACATCCTGTAGTAGTTCTAAACTTCCGTTGCGTGGAAGTGGGGTTTCAGCTTCACAATCATCCAACCATCGCATTGGAAGGAAAAGGAATCATATTTCTGATGTCCCAGCTTCTGTGAGTTTGGAGGCAAGCCGCAAAGAATTTGTTTTTCCAAAGTTAACTACCAGCCCTCTTCGTAGGTTTCAGTTAATTGATTCCGATTCTGATGATCCTTCAGTCAGTCATAGTGTTAGGAGGGCTACTTCTAAGGTTGACTCATCATCAAAGAAGCAACACACAAACCCTGGTCATTCTGCAACTACTAGTGGTACAAACAAAAATGCATCTACAGGTATGCCTGAAGATGTTGATTTGTGGAAAAACTTTAGTCCAATCAAGAAGTTTAACATTCCAACACCTGCTTTGGATGAGGTATGTCAAGAATACTTCCAATCTGTGAAGGATAAGATTACTCAAAATCTGGAGAGGGATGCATATCTGCGTACCAACGAGAATTTTCATGAAACGACACCCAGTGGTCAAGATGTTGAACAACTCTGGAATGTGGCTGATCCCCTTCCACCTGCCCATCATTACTTTTTCCATGATGATCCAAAGATTCGAAAGTTAGTCTGCAGTCGCTTGCTGAATTTTTCCCCCTTGGGTGCTGTTAGTGTCGGTGGAAATCAACAGACTGGTGTATCAGTTATTGATTACATGGGTCAATTTAGCAATGGAGAAGCTTCAAAACGGACAGTGAATCGGAGACAAAATATTTCAAACATATCAAATGTTGAAGAAGTCTTGCATGGTTCTGGAAACTGGGTGAACCCAAAAGGTAAAGCTAAACAGAAAGGCAGTGTCAAGCGGAGTGTAAACAAGGGAAGAAATAAGTCGGTCAAATCAAACACTGGGAACATGGAGCATGCTTCTGGAAACTGGGTGGAACCCAGAACCTCTGCCAGCAAAAAGCGGATTCAAGCAAATGCACAATCTTCTGGTGAATGGTACACACCTAGTCAAAGTGGTCAACCTAGCGGGCAAGCTGGTGGTCATTGGTATACAGGCCCTGGTGGAAGGAAGGTTTATGTTAGTAAAACTGGGCAGGAATTGACAGGTCAAACAGCCTACAGACTGTATTCAAAGAACATTGATGCCAAGATCAATTTACAGTTTGTCAGAACAGAAGGAACTAATGAAGCTGGAAAAACAGTGATTTGTCCAGCATCAATCCGGTTTTTACTCTTTTCTCTTTATAAGGTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

511

Amino Acids

56.53

Weight (kDa)

9.1

Isoelectric Point (pI)

54.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1524
AarI CACCTGC 2 cut(s) 694, 859
Acc36I ACCTGC 2 cut(s) 694, 859
AccI GTMKAC 1 cut(s) 1328
AccII CGCG 2 cut(s) 100, 142
AclWI GGATC 4 cut(s) 129, 491, 833, 872
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 428, 781, 916
AcuI CTGAAG 2 cut(s) 486, 648
AcyI GRCGYC 1 cut(s) 197
AdeI CACNNNGTG 1 cut(s) 803
AfaI GTAC 4 cut(s) 284, 598, 772, 1277
AfiI CCNNNNNNNGG 1 cut(s) 320
AflII CTTAAG 1 cut(s) 158
AgsI TTSAA 7 cut(s) 215, 818, 1014, 1047, 1064, 1247, 1410
AhlI ACTAGT 1 cut(s) 590
AjnI CCWGG 2 cut(s) 571, 1336
AleI CACNNNNGTG 1 cut(s) 116
AluBI AGCT 9 cut(s) 64, 74, 148, 338, 398, 1010, 1112, 1313, 1469
AluI AGCT 9 cut(s) 64, 74, 148, 338, 398, 1010, 1112, 1313, 1469
Alw21I GWGCWC 2 cut(s) 66, 150
Alw26I GTCTC 1 cut(s) 1027
AlwI GGATC 4 cut(s) 129, 491, 833, 872
AlwNI CAGNNNCTG 2 cut(s) 401, 1337
AoxI GGCC 2 cut(s) 3, 1333
ApoI RAATTY 3 cut(s) 428, 781, 916
AspS9I GGNCC 1 cut(s) 1334
AsuHPI GGTGA 2 cut(s) 1105, 1280
AsuII TTCGAA 2 cut(s) 54, 889
BaeI ACNNNNGTAYC 2 cut(s) 951, 984
BanII GRGCYC 3 cut(s) 66, 150, 189
Bbv12I GWGCWC 2 cut(s) 66, 150
BccI CCATC 2 cut(s) 363, 864
BciT130I CCWGG 2 cut(s) 573, 1338
BcoDI GTCTC 1 cut(s) 1027
BcuI ACTAGT 1 cut(s) 590
BfaI CTAG 3 cut(s) 591, 1284, 1302
BfmI CTRYAG 4 cut(s) 295, 615, 901, 1396
BfrI CTTAAG 1 cut(s) 158
BfuAI ACCTGC 2 cut(s) 694, 859
BisI GCNGC 4 cut(s) 6, 137, 140, 421
BlsI GCNGC 4 cut(s) 7, 138, 141, 422
Bme1390I CCNGG 2 cut(s) 573, 1338
BmgT120I GGNCC 1 cut(s) 1334
BmiI GGNNCC 2 cut(s) 186, 1215
BmrFI CCNGG 2 cut(s) 573, 1338
BmrI ACTGGG 5 cut(s) 795, 1099, 1188, 1216, 1377
BmsI GCATC 4 cut(s) 620, 748, 1411, 1502
BmuI ACTGGG 5 cut(s) 795, 1099, 1188, 1216, 1377
BoxI GACNNNNGTC 1 cut(s) 90
BpmI CTGGAG 2 cut(s) 73, 770
Bpu14I TTCGAA 2 cut(s) 54, 889
BpuEI CTTGAG 1 cut(s) 134
BsaBI GATNNNNATC 2 cut(s) 120, 762
BsaHI GRCGYC 1 cut(s) 197
BsaJI CCNNGG 4 cut(s) 140, 571, 927, 1336
BsaWI WCCGGW 1 cut(s) 1500
BsaXI ACNNNNNCTCC 2 cut(s) 61, 91
Bsc4I CCNNNNNNNGG 1 cut(s) 320
Bse1I ACTGG 7 cut(s) 90, 801, 967, 1094, 1183, 1211, 1372
Bse3DI GCAATG 1 cut(s) 1006
Bse8I GATNNNNATC 2 cut(s) 120, 762
BseBI CCWGG 2 cut(s) 573, 1338
BseDI CCNNGG 4 cut(s) 140, 571, 927, 1336
BseGI GGATG 4 cut(s) 290, 347, 700, 763
BseJI GATNNNNATC 2 cut(s) 120, 762
BseLI CCNNNNNNNGG 1 cut(s) 320
BseMI GCAATG 1 cut(s) 1006
BseNI ACTGG 7 cut(s) 90, 801, 967, 1094, 1183, 1211, 1372
BseRI GAGGAG 1 cut(s) 66
BseYI CCCAGC 1 cut(s) 394
BsgI GTGCAG 1 cut(s) 279
Bsh1236I CGCG 2 cut(s) 100, 142
BshFI GGCC 2 cut(s) 5, 1335
BsiHKAI GWGCWC 2 cut(s) 66, 150
BsiSI CCGG 2 cut(s) 173, 1501
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 1 cut(s) 320
BsmAI GTCTC 1 cut(s) 1027
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 2 cut(s) 5, 1335
Bsp119I TTCGAA 2 cut(s) 54, 889
Bsp1286I GDGCHC 3 cut(s) 66, 150, 189
Bsp143I GATC 6 cut(s) 121, 129, 496, 838, 877, 1428
BspANI GGCC 2 cut(s) 5, 1335
BspFNI CGCG 2 cut(s) 100, 142
BspHI TCATGA 1 cut(s) 787
BspLI GGNNCC 2 cut(s) 186, 1215
BspMAI CTGCAG 1 cut(s) 905
BspMI ACCTGC 2 cut(s) 694, 859
BspPI GGATC 4 cut(s) 129, 491, 833, 872
BspT104I TTCGAA 2 cut(s) 54, 889
BspTI CTTAAG 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 1006
BsrI ACTGG 7 cut(s) 90, 801, 967, 1094, 1183, 1211, 1372
BssECI CCNNGG 4 cut(s) 140, 571, 927, 1336
BssMI GATC 6 cut(s) 121, 129, 496, 838, 877, 1428
BssNAI GTATAC 1 cut(s) 1329
BssNI GRCGYC 1 cut(s) 197
BssT1I CCWWGG 1 cut(s) 927
Bst1107I GTATAC 1 cut(s) 1329
Bst2UI CCWGG 2 cut(s) 573, 1338
Bst4CI ACNGT 6 cut(s) 273, 282, 1024, 1404, 1440, 1480
Bst6I CTCTTC 3 cut(s) 222, 241, 465
BstACI GRCGYC 1 cut(s) 197
BstAFI CTTAAG 1 cut(s) 158
BstBI TTCGAA 2 cut(s) 54, 889
BstC8I GCNNGC 6 cut(s) 418, 911, 1194, 1231, 1307, 1311
BstDEI CTNAG 1 cut(s) 534
BstDSI CCRYGG 1 cut(s) 140
BstF5I GGATG 4 cut(s) 290, 347, 700, 763
BstFNI CGCG 2 cut(s) 100, 142
BstKTI GATC 6 cut(s) 124, 132, 499, 841, 880, 1431
BstMAI GTCTC 1 cut(s) 1027
BstMBI GATC 6 cut(s) 121, 129, 496, 838, 877, 1428
BstMWI GCNNNNNNNGC 4 cut(s) 145, 154, 169, 178
BstNI CCWGG 2 cut(s) 573, 1338
BstNSI RCATGY 1 cut(s) 1196
BstPAI GACNNNNGTC 1 cut(s) 90
BstSCI CCNGG 2 cut(s) 571, 1336
BstSFI CTRYAG 4 cut(s) 295, 615, 901, 1396
BstUI CGCG 2 cut(s) 100, 142
BstXI CCANNNNNNTGG 1 cut(s) 362
BstZ17I GTATAC 1 cut(s) 1329
BsuRI GGCC 2 cut(s) 5, 1335
BtgI CCRYGG 1 cut(s) 140
BtgZI GCGATG 1 cut(s) 341
BtsCI GGATG 4 cut(s) 290, 347, 700, 763
BtsI GCAGTG 1 cut(s) 1132
BtsIMutI CAGTG 6 cut(s) 111, 808, 1029, 1132, 1176, 1485
BveI ACCTGC 2 cut(s) 694, 859
Cac8I GCNNGC 6 cut(s) 418, 911, 1194, 1231, 1307, 1311
CaiI CAGNNNCTG 2 cut(s) 401, 1337
CciI TCATGA 1 cut(s) 787
Cfr13I GGNCC 1 cut(s) 1334
Cfr42I CCGCGG 1 cut(s) 143
CseI GACGC 2 cut(s) 89, 205
Csp6I GTAC 4 cut(s) 283, 597, 771, 1276
CviAII CATG 6 cut(s) 788, 872, 985, 1076, 1186, 1193
CviQI GTAC 4 cut(s) 283, 597, 771, 1276
DdeI CTNAG 1 cut(s) 534
DpnI GATC 6 cut(s) 123, 131, 498, 840, 879, 1430
DpnII GATC 6 cut(s) 121, 129, 496, 838, 877, 1428
DraIII CACNNNGTG 1 cut(s) 803
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 222, 241, 465
EarI CTCTTC 3 cut(s) 222, 241, 465
EciI GGCGGA 1 cut(s) 69
Ecl136II GAGCTC 2 cut(s) 64, 148
Eco130I CCWWGG 1 cut(s) 927
Eco24I GRGCYC 3 cut(s) 66, 150, 189
Eco53kI GAGCTC 2 cut(s) 64, 148
Eco57I CTGAAG 2 cut(s) 486, 648
EcoICRI GAGCTC 2 cut(s) 64, 148
EcoO109I RGGNCCY 1 cut(s) 1334
EcoRII CCWGG 2 cut(s) 571, 1336
EcoT14I CCWWGG 1 cut(s) 927
EcoT22I ATGCAT 2 cut(s) 613, 763
EcoT38I GRGCYC 3 cut(s) 66, 150, 189
ErhI CCWWGG 1 cut(s) 927
FaeI CATG 6 cut(s) 791, 875, 988, 1079, 1189, 1196
FaqI GGGAC 1 cut(s) 377
FatI CATG 6 cut(s) 787, 871, 984, 1075, 1185, 1192
FauI CCCGC 3 cut(s) 135, 174, 1298
FblI GTMKAC 1 cut(s) 1328
Fnu4HI GCNGC 4 cut(s) 6, 137, 140, 421
FokI GGATG 4 cut(s) 277, 334, 707, 770
FriOI GRGCYC 3 cut(s) 66, 150, 189
Fsp4HI GCNGC 4 cut(s) 6, 137, 140, 421
FspBI CTAG 3 cut(s) 591, 1284, 1302
GluI GCNGC 4 cut(s) 6, 137, 140, 421
GsaI CCCAGC 1 cut(s) 398
GsuI CTGGAG 2 cut(s) 73, 770
HaeIII GGCC 2 cut(s) 5, 1335
HapII CCGG 2 cut(s) 173, 1501
HgaI GACGC 2 cut(s) 89, 205
Hin1I GRCGYC 1 cut(s) 197
Hin1II CATG 6 cut(s) 791, 875, 988, 1079, 1189, 1196
HincII GTYRAC 5 cut(s) 109, 276, 447, 541, 1298
HindII GTYRAC 5 cut(s) 109, 276, 447, 541, 1298
HindIII AAGCTT 1 cut(s) 1008
HpaI GTTAAC 1 cut(s) 447
HpaII CCGG 2 cut(s) 173, 1501
HphI GGTGA 2 cut(s) 1105, 1280
Hpy166II GTNNAC 9 cut(s) 109, 276, 447, 541, 1096, 1144, 1278, 1298, 1329
Hpy188I TCNGA 7 cut(s) 38, 61, 388, 487, 493, 1032, 1448
Hpy8I GTNNAC 9 cut(s) 109, 276, 447, 541, 1096, 1144, 1278, 1298, 1329
Hpy99I CGWCG 2 cut(s) 196, 199
HpyAV CCTTC 8 cut(s) 33, 360, 510, 721, 854, 1338, 1342, 1448
HpyCH4III ACNGT 6 cut(s) 273, 282, 1024, 1404, 1440, 1480
HpyCH4V TGCA 8 cut(s) 260, 268, 584, 611, 761, 903, 1075, 1256
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 154, 169, 178
HpyF3I CTNAG 1 cut(s) 534
Hsp92I GRCGYC 1 cut(s) 197
Hsp92II CATG 6 cut(s) 791, 875, 988, 1079, 1189, 1196
KspAI GTTAAC 1 cut(s) 447
KspI CCGCGG 1 cut(s) 143
Kzo9I GATC 6 cut(s) 121, 129, 496, 838, 877, 1428
LmnI GCTCC 6 cut(s) 61, 69, 79, 145, 184, 1189
LweI GCATC 4 cut(s) 620, 748, 1411, 1502
MaeI CTAG 3 cut(s) 591, 1284, 1302
MalI GATC 6 cut(s) 123, 131, 498, 840, 879, 1430
MboI GATC 6 cut(s) 121, 129, 496, 838, 877, 1428
MboII GAAGA 9 cut(s) 227, 228, 239, 257, 452, 641, 1076, 1164, 1254
MhlI GDGCHC 3 cut(s) 66, 150, 189
MluCI AATT 7 cut(s) 428, 477, 781, 916, 992, 1376, 1432
MlyI GAGTC 3 cut(s) 33, 80, 536
MmeI TCCRAC 2 cut(s) 375, 704
Mph1103I ATGCAT 2 cut(s) 613, 763
MseI TTAA 4 cut(s) 159, 446, 476, 672
MslI CAYNNNNRTG 1 cut(s) 116
MspA1I CMGCKG 1 cut(s) 142
MspCI CTTAAG 1 cut(s) 158
MspI CCGG 2 cut(s) 173, 1501
MspR9I CCNGG 2 cut(s) 573, 1338
MvaI CCWGG 2 cut(s) 573, 1338
MvnI CGCG 2 cut(s) 100, 142
MwoI GCNNNNNNNGC 4 cut(s) 145, 154, 169, 178
NdeII GATC 6 cut(s) 121, 129, 496, 838, 877, 1428
NlaIII CATG 6 cut(s) 791, 875, 988, 1079, 1189, 1196
NlaIV GGNNCC 2 cut(s) 186, 1215
NsiI ATGCAT 2 cut(s) 613, 763
NspI RCATGY 1 cut(s) 1196
NspV TTCGAA 2 cut(s) 54, 889
OliI CACNNNNGTG 1 cut(s) 116
PaeI GCATGC 1 cut(s) 1196
PagI TCATGA 1 cut(s) 787
PaqCI CACCTGC 2 cut(s) 694, 859
PfeI GAWTC 7 cut(s) 56, 376, 482, 488, 886, 1027, 1243
PkrI GCNGC 4 cut(s) 7, 138, 141, 422
PleI GAGTC 3 cut(s) 33, 80, 536
PpsI GAGTC 3 cut(s) 33, 80, 536
PshAI GACNNNNGTC 1 cut(s) 90
PsiI TTATAA 1 cut(s) 1524
Psp124BI GAGCTC 2 cut(s) 66, 150
Psp6I CCWGG 2 cut(s) 571, 1336
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 2 cut(s) 571, 1336
PspN4I GGNNCC 2 cut(s) 186, 1215
PspPI GGNCC 1 cut(s) 1334
PstI CTGCAG 1 cut(s) 905
PstNI CAGNNNCTG 2 cut(s) 401, 1337
RsaI GTAC 4 cut(s) 284, 598, 772, 1277
RsaNI GTAC 4 cut(s) 283, 597, 771, 1276
RseI CAYNNNNRTG 1 cut(s) 116
SacI GAGCTC 2 cut(s) 66, 150
SacII CCGCGG 1 cut(s) 143
SaqAI TTAA 4 cut(s) 159, 446, 476, 672
SatI GCNGC 4 cut(s) 6, 137, 140, 421
Sau3AI GATC 6 cut(s) 121, 129, 496, 838, 877, 1428
Sau96I GGNCC 1 cut(s) 1334
SchI GAGTC 3 cut(s) 33, 80, 536
ScrFI CCNGG 2 cut(s) 573, 1338
SduI GDGCHC 3 cut(s) 66, 150, 189
SfaNI GCATC 4 cut(s) 620, 748, 1411, 1502
SfcI CTRYAG 4 cut(s) 295, 615, 901, 1396
Sfr303I CCGCGG 1 cut(s) 143
SfuI TTCGAA 2 cut(s) 54, 889
SgrBI CCGCGG 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 116
SmlI CTYRAG 2 cut(s) 149, 158
SmoI CTYRAG 2 cut(s) 149, 158
SpeI ACTAGT 1 cut(s) 590
SphI GCATGC 1 cut(s) 1196
Sse9I AATT 7 cut(s) 428, 477, 781, 916, 992, 1376, 1432
SspI AATATT 2 cut(s) 211, 1042
SspMI CTAG 3 cut(s) 591, 1284, 1302
SstI GAGCTC 2 cut(s) 66, 150
StyD4I CCNGG 2 cut(s) 571, 1336
StyI CCWWGG 1 cut(s) 927
TaaI ACNGT 6 cut(s) 273, 282, 1024, 1404, 1440, 1480
TaqI TCGA 6 cut(s) 42, 54, 124, 132, 225, 889
TaqII GACCGA 1 cut(s) 1153
TasI AATT 7 cut(s) 428, 477, 781, 916, 992, 1376, 1432
TauI GCSGC 4 cut(s) 8, 139, 142, 423
TfiI GAWTC 7 cut(s) 56, 376, 482, 488, 886, 1027, 1243
Tru1I TTAA 4 cut(s) 159, 446, 476, 672
Tru9I TTAA 4 cut(s) 159, 446, 476, 672
TscAI CASTG 6 cut(s) 118, 808, 1029, 1132, 1183, 1485
TspDTI ATGAA 3 cut(s) 776, 804, 1479
TspGWI ACGGA 2 cut(s) 303, 1033
TspRI CASTG 6 cut(s) 118, 808, 1029, 1132, 1183, 1485
Vha464I CTTAAG 1 cut(s) 158
XapI RAATTY 3 cut(s) 428, 781, 916
XceI RCATGY 1 cut(s) 1196
XmiI GTMKAC 1 cut(s) 1328
XspI CTAG 3 cut(s) 591, 1284, 1302
Zsp2I ATGCAT 2 cut(s) 613, 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.