Rh5DG277700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005748
Physical Location & Seq
Reverse (-)
33985998 .. 33987354
1357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG277700.1

Sequence Viewer

Length: 753 bp
ATGCATCTACAAGTATGCCTGAAGACGTTGATTTGTGGAAAAACTTTAGTCCAATCAAGAAGTTTAACATTCGAACACCTGCTATGTCAAGAATACTTCCAATATGTGAAGGATAAGACTGCTCAAAATCTGGAGAGGGATGCATATCTGCATACCAACGAGAATTTTCATGAAACGACACCCAATGGTCAAGATGTTGAACAACTCTGGAATGTGGCTGATCCCCTTCCATCCGCCCATCATTACTTTTTCCACGATGATCCAAAGATTCGAAAGGGTCAATTTAGCAATGGAGAAGCTTCAAAACGGACAGTGAATAGGAGACAAAGTATTTCAAACATATCAAATGTTGAAGAAGTCTTGCATGGTTATGGAAACTGGGTGAACCCAAACGATAAAGCTAAACAGATAGGTAGTGTCAAGCGGAGTGTAAACAAGGGAATAAATAAGTTGGTCAAATCAAACACCGGGAACATGGAGCATGATTCTGGAAACTGGGTGGAACCCAGAATCTCTGCCAGCAAAAAGCGGATTCAAGCAAACGCACAATCTTCTGGTGAATGGTACACACCTAGTCAAAGTGGTCAAGCTAGTGGGCAAGCTGGTGGTCATTGGTATACAGGCCCTGGTGGAAGGAAGGTTTATGTTAGTAAAACTGGGCAGGAATTGACAGGTCAAACAGCCTACAGACTGTACTCAAAGGAAAGTGGGAAACGGGCTGTGAAGGCCAGAAAAGGGAAAGCCAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

250

Amino Acids

28.09

Weight (kDa)

9.7

Isoelectric Point (pI)

34.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 87
Acc36I ACCTGC 1 cut(s) 87
AccI GTMKAC 1 cut(s) 617
AciI CCGC 3 cut(s) 234, 424, 529
AclWI GGATC 2 cut(s) 215, 254
AcsI RAATTY 1 cut(s) 163
AcuI CTGAAG 1 cut(s) 41
AfaI GTAC 2 cut(s) 566, 695
AfiI CCNNNNNNNGG 1 cut(s) 735
AgsI TTSAA 5 cut(s) 200, 303, 336, 353, 536
AjnI CCWGG 1 cut(s) 625
AluBI AGCT 4 cut(s) 299, 401, 590, 602
AluI AGCT 4 cut(s) 299, 401, 590, 602
Alw26I GTCTC 1 cut(s) 316
AlwI GGATC 2 cut(s) 215, 254
AlwNI CAGNNNCTG 1 cut(s) 626
AoxI GGCC 2 cut(s) 622, 726
ApoI RAATTY 1 cut(s) 163
AspS9I GGNCC 1 cut(s) 623
AsuC2I CCSGG 1 cut(s) 469
AsuHPI GGTGA 2 cut(s) 394, 569
AsuII TTCGAA 2 cut(s) 72, 271
BbsI GAAGAC 1 cut(s) 29
BccI CCATC 2 cut(s) 238, 246
BciT130I CCWGG 1 cut(s) 627
BcnI CCSGG 1 cut(s) 469
BcoDI GTCTC 1 cut(s) 316
BfaI CTAG 2 cut(s) 573, 591
BfmI CTRYAG 1 cut(s) 685
BfuAI ACCTGC 1 cut(s) 87
Bme1390I CCNGG 2 cut(s) 469, 627
BmgT120I GGNCC 1 cut(s) 623
BmiI GGNNCC 1 cut(s) 504
BmrFI CCNGG 2 cut(s) 469, 627
BmrI ACTGGG 3 cut(s) 388, 505, 666
BmsI GCATC 2 cut(s) 13, 130
BmuI ACTGGG 3 cut(s) 388, 505, 666
BpiI GAAGAC 1 cut(s) 29
BpmI CTGGAG 1 cut(s) 152
Bpu14I TTCGAA 2 cut(s) 72, 271
BpuMI CCSGG 1 cut(s) 469
BsaBI GATNNNNATC 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 625
BsaXI ACNNNNNCTCC 2 cut(s) 313, 343
Bsc4I CCNNNNNNNGG 1 cut(s) 735
Bse1I ACTGG 3 cut(s) 383, 500, 661
Bse3DI GCAATG 1 cut(s) 295
Bse8I GATNNNNATC 1 cut(s) 144
BseBI CCWGG 1 cut(s) 627
BseDI CCNNGG 1 cut(s) 625
BseGI GGATG 2 cut(s) 145, 230
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 735
BseMI GCAATG 1 cut(s) 295
BseNI ACTGG 3 cut(s) 383, 500, 661
BshFI GGCC 2 cut(s) 624, 728
BsiSI CCGG 1 cut(s) 468
BslI CCNNNNNNNGG 1 cut(s) 735
BsmAI GTCTC 1 cut(s) 316
BsnI GGCC 2 cut(s) 624, 728
Bsp119I TTCGAA 2 cut(s) 72, 271
Bsp143I GATC 2 cut(s) 220, 259
BspACI CCGC 3 cut(s) 234, 424, 529
BspANI GGCC 2 cut(s) 624, 728
BspHI TCATGA 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 504
BspMI ACCTGC 1 cut(s) 87
BspPI GGATC 2 cut(s) 215, 254
BspT104I TTCGAA 2 cut(s) 72, 271
BsrDI GCAATG 1 cut(s) 295
BsrI ACTGG 3 cut(s) 383, 500, 661
BssECI CCNNGG 1 cut(s) 625
BssMI GATC 2 cut(s) 220, 259
BssNAI GTATAC 1 cut(s) 618
Bst1107I GTATAC 1 cut(s) 618
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 2 cut(s) 313, 693
BstBI TTCGAA 2 cut(s) 72, 271
BstC8I GCNNGC 2 cut(s) 520, 600
BstF5I GGATG 2 cut(s) 145, 230
BstKTI GATC 2 cut(s) 223, 262
BstMAI GTCTC 1 cut(s) 316
BstMBI GATC 2 cut(s) 220, 259
BstMWI GCNNNNNNNGC 1 cut(s) 725
BstNI CCWGG 1 cut(s) 627
BstSCI CCNGG 2 cut(s) 467, 625
BstSFI CTRYAG 1 cut(s) 685
BstV2I GAAGAC 1 cut(s) 29
BstZ17I GTATAC 1 cut(s) 618
BsuRI GGCC 2 cut(s) 624, 728
BtsCI GGATG 2 cut(s) 145, 230
BtsIMutI CAGTG 1 cut(s) 318
BveI ACCTGC 1 cut(s) 87
Cac8I GCNNGC 2 cut(s) 520, 600
CaiI CAGNNNCTG 1 cut(s) 626
CciI TCATGA 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 623
Csp6I GTAC 2 cut(s) 565, 694
CviAII CATG 4 cut(s) 170, 365, 475, 482
CviQI GTAC 2 cut(s) 565, 694
DpnI GATC 2 cut(s) 222, 261
DpnII GATC 2 cut(s) 220, 259
EciI GGCGGA 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 623
EcoRII CCWGG 1 cut(s) 625
EcoT22I ATGCAT 2 cut(s) 6, 145
FaeI CATG 4 cut(s) 173, 368, 478, 485
FatI CATG 4 cut(s) 169, 364, 474, 481
FblI GTMKAC 1 cut(s) 617
FokI GGATG 2 cut(s) 152, 217
FspBI CTAG 2 cut(s) 573, 591
GsuI CTGGAG 1 cut(s) 152
HaeIII GGCC 2 cut(s) 624, 728
HapII CCGG 1 cut(s) 468
Hin1II CATG 4 cut(s) 173, 368, 478, 485
HindIII AAGCTT 1 cut(s) 297
HinfI GANTC 4 cut(s) 268, 485, 510, 532
HpaII CCGG 1 cut(s) 468
HphI GGTGA 2 cut(s) 394, 569
Hpy166II GTNNAC 4 cut(s) 385, 433, 567, 618
Hpy188III TCNNGA 7 cut(s) 57, 89, 131, 170, 191, 208, 489
Hpy8I GTNNAC 4 cut(s) 385, 433, 567, 618
HpyAV CCTTC 5 cut(s) 103, 236, 627, 631, 718
HpyCH4III ACNGT 2 cut(s) 313, 693
HpyCH4IV ACGT 1 cut(s) 26
HpyCH4V TGCA 4 cut(s) 4, 143, 151, 364
HpyF10VI GCNNNNNNNGC 1 cut(s) 725
HpySE526I ACGT 1 cut(s) 26
Hsp92II CATG 4 cut(s) 173, 368, 478, 485
Kzo9I GATC 2 cut(s) 220, 259
LmnI GCTCC 1 cut(s) 478
LweI GCATC 2 cut(s) 13, 130
MaeI CTAG 2 cut(s) 573, 591
MaeII ACGT 1 cut(s) 26
MalI GATC 2 cut(s) 222, 261
MboI GATC 2 cut(s) 220, 259
MboII GAAGA 3 cut(s) 34, 365, 543
MluCI AATT 3 cut(s) 163, 281, 665
MnlI CCTC 1 cut(s) 129
Mph1103I ATGCAT 2 cut(s) 6, 145
MseI TTAA 1 cut(s) 65
MslI CAYNNNNRTG 1 cut(s) 369
MspI CCGG 1 cut(s) 468
MspR9I CCNGG 2 cut(s) 469, 627
MvaI CCWGG 1 cut(s) 627
MwoI GCNNNNNNNGC 1 cut(s) 725
NciI CCSGG 1 cut(s) 469
NdeII GATC 2 cut(s) 220, 259
NlaIII CATG 4 cut(s) 173, 368, 478, 485
NlaIV GGNNCC 1 cut(s) 504
NsiI ATGCAT 2 cut(s) 6, 145
NspV TTCGAA 2 cut(s) 72, 271
PagI TCATGA 1 cut(s) 169
PaqCI CACCTGC 1 cut(s) 87
PfeI GAWTC 4 cut(s) 268, 485, 510, 532
Psp6I CCWGG 1 cut(s) 625
PspGI CCWGG 1 cut(s) 625
PspN4I GGNNCC 1 cut(s) 504
PspPI GGNCC 1 cut(s) 623
PstNI CAGNNNCTG 1 cut(s) 626
RsaI GTAC 2 cut(s) 566, 695
RsaNI GTAC 2 cut(s) 565, 694
RseI CAYNNNNRTG 1 cut(s) 369
SaqAI TTAA 1 cut(s) 65
Sau3AI GATC 2 cut(s) 220, 259
Sau96I GGNCC 1 cut(s) 623
ScrFI CCNGG 2 cut(s) 469, 627
SfaNI GCATC 2 cut(s) 13, 130
SfcI CTRYAG 1 cut(s) 685
SfuI TTCGAA 2 cut(s) 72, 271
SmiMI CAYNNNNRTG 1 cut(s) 369
Sse9I AATT 3 cut(s) 163, 281, 665
SsiI CCGC 3 cut(s) 234, 424, 529
SspMI CTAG 2 cut(s) 573, 591
StyD4I CCNGG 2 cut(s) 467, 625
TaaI ACNGT 2 cut(s) 313, 693
TaiI ACGT 1 cut(s) 29
TaqI TCGA 2 cut(s) 72, 271
TasI AATT 3 cut(s) 163, 281, 665
TatI WGTACW 1 cut(s) 693
TfiI GAWTC 4 cut(s) 268, 485, 510, 532
Tru1I TTAA 1 cut(s) 65
Tru9I TTAA 1 cut(s) 65
TscAI CASTG 1 cut(s) 318
TspDTI ATGAA 2 cut(s) 158, 186
TspGWI ACGGA 1 cut(s) 322
TspRI CASTG 1 cut(s) 318
XapI RAATTY 1 cut(s) 163
XmiI GTMKAC 1 cut(s) 617
XspI CTAG 2 cut(s) 573, 591
Zsp2I ATGCAT 2 cut(s) 6, 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.