Rw5G024880

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005748
Physical Location & Seq
Reverse (-)
35291208 .. 35294876
3669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G024880.1

Sequence Viewer

Length: 1443 bp
ATGGCCGCCGACTACGAACCTCCTTCTTTCTCTCTCGGACTCGATTTCGGGTTCGAATCGGAGCTCCAAACAGCTGCTCCGCCCGACTCCAGTCCTCCGCGTCCTGTTGACACTGATGTGGATCGAGAGATCGAGCCGCCGCGGGAGCTCAAGAGGCTGAAGCGAGGTCTGCCGGAGAAGCGGGAGCCGCCGACGACGCCGTTTTGGAATATTGAAGATGACATCGAAGAGTTCTCTTCTCCCGAAGATACTATTCCAGCAGATGTGCAACGGTCAACACAGTACCAAACATCCTGTAGTAGTTCTAAACTTCCGTTGCGTGGAAGTGGGGTTTTAGCTTCACAATCATCCAACCATCGCATTGGAAGGAAAAGGAATCATATTTCTGATGTCCCAGCTTCTGTGAGTTTGGAGGCAAGCCGCAAAGAATTTGTTTTTCCAAAGTTAACTACCAGCCCTCTTCGTAGGTTTCAGTTAATTGATTCCGATTCTGATGATCCTTCAGTCAGTCATAGTGTTAGTAGGGTTACTTCTAAGGTTGACTCATCATCAAAGAAGCAACACACAAACCCTGGTCATTCTGCAACTACTAGTGGTACAAACAAAAATGCATCTACAGGTATGCCTGAAGATGTTGATTTGTGGAAAAACTTTAGTCCAATCAAGAAGTTTAACATTCCAACACCTGCTTTGGATGAGGTATGTCAAGAATACTTCCAATCTGTGAAGGATAAGATTACTCAAAATCTGGAGAGGGATGCATATCTGCGTACCAACGAGAATTTTCATGAAACGACACCCAGTGGTCAAGATGTTGAACAACTCTGGAATGTGGCTGATCCCCTTCCATCCGCCCATCATTACTTTTTCCACGATGATCCAAAGATTCGGAAGTTAGTCTGTAGTCGCTTGCCGAATTTTTCCCCCTTAGTGTCGGTGGAAATCAACAGACTGGTGTATCAGGGTCAATTTAGCAATGGAGAAGCTTCAAAACGGACTGTGAATCGGAGACAAAATATTTCAAACATATCAAATGTTGAAGAAGTCTTGCATGGTTCTGGAAACTGGGTGAACCCAAAAGGTAAAGCTAAACAGAAAGGTAGTGTCAAGCGGAGTCATGCTTCTGGAAACTGGGTGGAACCCAGAATCTCTGCCAGCAAAAAGCGGATTCAAGCAAATGCACAATCTTCTGGTGAATGGTACACACCTAGTCAAAGTGGTCAACCTAGCGGGCAAGCTGGTGGTCATTGGTATACAGGCCCTGGTGGAAGGAAGGTTTATGTTAGTAAAACTGGGCAGGAATTGACAGGTCAAACAGCCTACAGACTGTATTCAAAGAGTTACCCACCTCAAATTGCAGTAGCCCAGACTGCCAAAAAGCTTTACCCCAAGAAAAAGAAAAGAAAAGAATTGATACTCTATACATATCTGTTTCATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

480

Amino Acids

53.75

Weight (kDa)

9.27

Isoelectric Point (pI)

56.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 694
Acc36I ACCTGC 1 cut(s) 694
AccI GTMKAC 1 cut(s) 1253
AccII CGCG 2 cut(s) 100, 142
AclWI GGATC 4 cut(s) 129, 491, 833, 872
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 428, 781, 916
AcuI CTGAAG 3 cut(s) 179, 486, 648
AcyI GRCGYC 1 cut(s) 197
AdeI CACNNNGTG 1 cut(s) 803
AfaI GTAC 4 cut(s) 284, 598, 772, 1202
AfiI CCNNNNNNNGG 1 cut(s) 320
AgsI TTSAA 7 cut(s) 215, 818, 990, 1023, 1040, 1172, 1335
AhlI ACTAGT 1 cut(s) 590
AjnI CCWGG 2 cut(s) 571, 1261
AleI CACNNNNGTG 1 cut(s) 116
AluBI AGCT 9 cut(s) 64, 74, 148, 338, 398, 986, 1088, 1238, 1381
AluI AGCT 9 cut(s) 64, 74, 148, 338, 398, 986, 1088, 1238, 1381
Alw21I GWGCWC 2 cut(s) 66, 150
Alw26I GTCTC 1 cut(s) 1003
AlwI GGATC 4 cut(s) 129, 491, 833, 872
AlwNI CAGNNNCTG 2 cut(s) 401, 1262
AoxI GGCC 2 cut(s) 3, 1258
ApeKI GCWGC 1 cut(s) 74
ApoI RAATTY 3 cut(s) 428, 781, 916
AspS9I GGNCC 1 cut(s) 1259
AsuHPI GGTGA 2 cut(s) 1081, 1205
AsuII TTCGAA 1 cut(s) 54
BaeI ACNNNNGTAYC 2 cut(s) 941, 974
BanII GRGCYC 2 cut(s) 66, 150
BarI GAAGNNNNNNTAC 2 cut(s) 514, 546
Bbv12I GWGCWC 2 cut(s) 66, 150
BbvI GCAGC 1 cut(s) 61
BccI CCATC 3 cut(s) 363, 856, 864
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 2 cut(s) 573, 1263
BcoDI GTCTC 1 cut(s) 1003
BcuI ACTAGT 1 cut(s) 590
BfaI CTAG 3 cut(s) 591, 1209, 1227
BfmI CTRYAG 4 cut(s) 295, 615, 901, 1321
BfuAI ACCTGC 1 cut(s) 694
BisI GCNGC 6 cut(s) 6, 75, 137, 140, 188, 421
BlsI GCNGC 6 cut(s) 7, 76, 138, 141, 189, 422
Bme1390I CCNGG 2 cut(s) 573, 1263
BmgT120I GGNCC 1 cut(s) 1259
BmiI GGNNCC 2 cut(s) 186, 1140
BmrFI CCNGG 2 cut(s) 573, 1263
BmrI ACTGGG 4 cut(s) 795, 1075, 1141, 1302
BmsI GCATC 2 cut(s) 620, 748
BmuI ACTGGG 4 cut(s) 795, 1075, 1141, 1302
BoxI GACNNNNGTC 1 cut(s) 90
BpmI CTGGAG 2 cut(s) 73, 770
Bpu14I TTCGAA 1 cut(s) 54
BpuEI CTTGAG 1 cut(s) 134
BsaBI GATNNNNATC 2 cut(s) 120, 762
BsaHI GRCGYC 1 cut(s) 197
BsaJI CCNNGG 3 cut(s) 140, 571, 1261
BsaXI ACNNNNNCTCC 2 cut(s) 61, 91
Bsc4I CCNNNNNNNGG 1 cut(s) 320
Bse1I ACTGG 6 cut(s) 90, 801, 957, 1070, 1136, 1297
Bse3DI GCAATG 1 cut(s) 982
Bse8I GATNNNNATC 2 cut(s) 120, 762
BseBI CCWGG 2 cut(s) 573, 1263
BseDI CCNNGG 3 cut(s) 140, 571, 1261
BseGI GGATG 5 cut(s) 290, 347, 700, 763, 848
BseJI GATNNNNATC 2 cut(s) 120, 762
BseLI CCNNNNNNNGG 1 cut(s) 320
BseMI GCAATG 1 cut(s) 982
BseNI ACTGG 6 cut(s) 90, 801, 957, 1070, 1136, 1297
BseXI GCAGC 1 cut(s) 61
BseYI CCCAGC 1 cut(s) 394
Bsh1236I CGCG 2 cut(s) 100, 142
BshFI GGCC 2 cut(s) 5, 1260
BsiHKAI GWGCWC 2 cut(s) 66, 150
BsiSI CCGG 1 cut(s) 173
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 1 cut(s) 320
BsmAI GTCTC 1 cut(s) 1003
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 2 cut(s) 5, 1260
Bsp119I TTCGAA 1 cut(s) 54
Bsp1286I GDGCHC 2 cut(s) 66, 150
Bsp143I GATC 5 cut(s) 121, 129, 496, 838, 877
BspANI GGCC 2 cut(s) 5, 1260
BspFNI CGCG 2 cut(s) 100, 142
BspHI TCATGA 1 cut(s) 787
BspLI GGNNCC 2 cut(s) 186, 1140
BspMI ACCTGC 1 cut(s) 694
BspPI GGATC 4 cut(s) 129, 491, 833, 872
BspT104I TTCGAA 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 982
BsrI ACTGG 6 cut(s) 90, 801, 957, 1070, 1136, 1297
BssECI CCNNGG 3 cut(s) 140, 571, 1261
BssMI GATC 5 cut(s) 121, 129, 496, 838, 877
BssNAI GTATAC 1 cut(s) 1254
BssNI GRCGYC 1 cut(s) 197
Bst1107I GTATAC 1 cut(s) 1254
Bst2UI CCWGG 2 cut(s) 573, 1263
Bst4CI ACNGT 4 cut(s) 273, 282, 1000, 1329
Bst6I CTCTTC 3 cut(s) 222, 241, 465
BstACI GRCGYC 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 54
BstC8I GCNNGC 5 cut(s) 418, 911, 1156, 1232, 1236
BstDEI CTNAG 2 cut(s) 534, 928
BstDSI CCRYGG 1 cut(s) 140
BstF5I GGATG 5 cut(s) 290, 347, 700, 763, 848
BstFNI CGCG 2 cut(s) 100, 142
BstKTI GATC 5 cut(s) 124, 132, 499, 841, 880
BstMAI GTCTC 1 cut(s) 1003
BstMBI GATC 5 cut(s) 121, 129, 496, 838, 877
BstMWI GCNNNNNNNGC 7 cut(s) 145, 154, 169, 178, 187, 196, 1370
BstNI CCWGG 2 cut(s) 573, 1263
BstPAI GACNNNNGTC 1 cut(s) 90
BstSCI CCNGG 2 cut(s) 571, 1261
BstSFI CTRYAG 4 cut(s) 295, 615, 901, 1321
BstUI CGCG 2 cut(s) 100, 142
BstV1I GCAGC 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 362
BstZ17I GTATAC 1 cut(s) 1254
BsuRI GGCC 2 cut(s) 5, 1260
BtgI CCRYGG 1 cut(s) 140
BtgZI GCGATG 1 cut(s) 341
BtsCI GGATG 5 cut(s) 290, 347, 700, 763, 848
BtsIMutI CAGTG 2 cut(s) 111, 808
BveI ACCTGC 1 cut(s) 694
Cac8I GCNNGC 5 cut(s) 418, 911, 1156, 1232, 1236
CaiI CAGNNNCTG 2 cut(s) 401, 1262
CciI TCATGA 1 cut(s) 787
Cfr13I GGNCC 1 cut(s) 1259
Cfr42I CCGCGG 1 cut(s) 143
CseI GACGC 2 cut(s) 89, 205
Csp6I GTAC 4 cut(s) 283, 597, 771, 1201
CviAII CATG 3 cut(s) 788, 1052, 1118
CviQI GTAC 4 cut(s) 283, 597, 771, 1201
DdeI CTNAG 2 cut(s) 534, 928
DpnI GATC 5 cut(s) 123, 131, 498, 840, 879
DpnII GATC 5 cut(s) 121, 129, 496, 838, 877
DraIII CACNNNGTG 1 cut(s) 803
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 222, 241, 465
EarI CTCTTC 3 cut(s) 222, 241, 465
EciI GGCGGA 2 cut(s) 69, 841
Ecl136II GAGCTC 2 cut(s) 64, 148
Eco24I GRGCYC 2 cut(s) 66, 150
Eco53kI GAGCTC 2 cut(s) 64, 148
Eco57I CTGAAG 3 cut(s) 179, 486, 648
EcoICRI GAGCTC 2 cut(s) 64, 148
EcoO109I RGGNCCY 1 cut(s) 1259
EcoRII CCWGG 2 cut(s) 571, 1261
EcoT22I ATGCAT 2 cut(s) 613, 763
EcoT38I GRGCYC 2 cut(s) 66, 150
FaeI CATG 3 cut(s) 791, 1055, 1121
FaqI GGGAC 1 cut(s) 377
FatI CATG 3 cut(s) 787, 1051, 1117
FauI CCCGC 3 cut(s) 135, 174, 1223
FblI GTMKAC 1 cut(s) 1253
Fnu4HI GCNGC 6 cut(s) 6, 75, 137, 140, 188, 421
FokI GGATG 5 cut(s) 277, 334, 707, 770, 835
FriOI GRGCYC 2 cut(s) 66, 150
Fsp4HI GCNGC 6 cut(s) 6, 75, 137, 140, 188, 421
FspBI CTAG 3 cut(s) 591, 1209, 1227
GluI GCNGC 6 cut(s) 6, 75, 137, 140, 188, 421
GsaI CCCAGC 1 cut(s) 398
GsuI CTGGAG 2 cut(s) 73, 770
HaeIII GGCC 2 cut(s) 5, 1260
HapII CCGG 1 cut(s) 173
HgaI GACGC 2 cut(s) 89, 205
Hin1I GRCGYC 1 cut(s) 197
Hin1II CATG 3 cut(s) 791, 1055, 1121
HincII GTYRAC 5 cut(s) 109, 276, 447, 541, 1223
HindII GTYRAC 5 cut(s) 109, 276, 447, 541, 1223
HindIII AAGCTT 2 cut(s) 984, 1379
HpaI GTTAAC 1 cut(s) 447
HpaII CCGG 1 cut(s) 173
HphI GGTGA 2 cut(s) 1081, 1205
Hpy166II GTNNAC 8 cut(s) 109, 276, 447, 541, 1072, 1203, 1223, 1254
Hpy188I TCNGA 7 cut(s) 38, 61, 388, 487, 493, 891, 1008
Hpy8I GTNNAC 8 cut(s) 109, 276, 447, 541, 1072, 1203, 1223, 1254
Hpy99I CGWCG 2 cut(s) 196, 199
HpyAV CCTTC 7 cut(s) 33, 360, 510, 721, 854, 1263, 1267
HpyCH4III ACNGT 4 cut(s) 273, 282, 1000, 1329
HpyCH4V TGCA 7 cut(s) 268, 584, 611, 761, 1051, 1181, 1358
HpyF10VI GCNNNNNNNGC 7 cut(s) 145, 154, 169, 178, 187, 196, 1370
HpyF3I CTNAG 2 cut(s) 534, 928
Hsp92I GRCGYC 1 cut(s) 197
Hsp92II CATG 3 cut(s) 791, 1055, 1121
KspAI GTTAAC 1 cut(s) 447
KspI CCGCGG 1 cut(s) 143
Kzo9I GATC 5 cut(s) 121, 129, 496, 838, 877
LmnI GCTCC 5 cut(s) 61, 69, 82, 145, 184
Lsp1109I GCAGC 1 cut(s) 61
LweI GCATC 2 cut(s) 620, 748
MaeI CTAG 3 cut(s) 591, 1209, 1227
MaeIII GTNAC 2 cut(s) 526, 1340
MalI GATC 5 cut(s) 123, 131, 498, 840, 879
MboI GATC 5 cut(s) 121, 129, 496, 838, 877
MboII GAAGA 8 cut(s) 227, 228, 239, 257, 452, 641, 1052, 1179
MhlI GDGCHC 2 cut(s) 66, 150
MluCI AATT 8 cut(s) 428, 477, 781, 916, 968, 1301, 1353, 1409
MlyI GAGTC 4 cut(s) 33, 80, 536, 1123
MmeI TCCRAC 2 cut(s) 375, 704
MnlI CCTC 9 cut(s) 30, 105, 147, 158, 406, 468, 691, 747, 1359
Mph1103I ATGCAT 2 cut(s) 613, 763
MseI TTAA 4 cut(s) 446, 476, 672, 1441
MslI CAYNNNNRTG 1 cut(s) 116
MspA1I CMGCKG 2 cut(s) 74, 142
MspI CCGG 1 cut(s) 173
MspR9I CCNGG 2 cut(s) 573, 1263
MvaI CCWGG 2 cut(s) 573, 1263
MvnI CGCG 2 cut(s) 100, 142
MwoI GCNNNNNNNGC 7 cut(s) 145, 154, 169, 178, 187, 196, 1370
NdeII GATC 5 cut(s) 121, 129, 496, 838, 877
NlaIII CATG 3 cut(s) 791, 1055, 1121
NlaIV GGNNCC 2 cut(s) 186, 1140
NsiI ATGCAT 2 cut(s) 613, 763
NspV TTCGAA 1 cut(s) 54
OliI CACNNNNGTG 1 cut(s) 116
PagI TCATGA 1 cut(s) 787
PaqCI CACCTGC 1 cut(s) 694
PfeI GAWTC 8 cut(s) 56, 376, 482, 488, 886, 1003, 1146, 1168
PkrI GCNGC 6 cut(s) 7, 76, 138, 141, 189, 422
PleI GAGTC 4 cut(s) 33, 80, 536, 1122
PpsI GAGTC 4 cut(s) 33, 80, 536, 1122
PshAI GACNNNNGTC 1 cut(s) 90
Psp124BI GAGCTC 2 cut(s) 66, 150
Psp6I CCWGG 2 cut(s) 571, 1261
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 2 cut(s) 571, 1261
PspN4I GGNNCC 2 cut(s) 186, 1140
PspPI GGNCC 1 cut(s) 1259
PstNI CAGNNNCTG 2 cut(s) 401, 1262
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 4 cut(s) 284, 598, 772, 1202
RsaNI GTAC 4 cut(s) 283, 597, 771, 1201
RseI CAYNNNNRTG 1 cut(s) 116
SacI GAGCTC 2 cut(s) 66, 150
SacII CCGCGG 1 cut(s) 143
SaqAI TTAA 4 cut(s) 446, 476, 672, 1441
SatI GCNGC 6 cut(s) 6, 75, 137, 140, 188, 421
Sau3AI GATC 5 cut(s) 121, 129, 496, 838, 877
Sau96I GGNCC 1 cut(s) 1259
SchI GAGTC 4 cut(s) 33, 80, 536, 1123
ScrFI CCNGG 2 cut(s) 573, 1263
SduI GDGCHC 2 cut(s) 66, 150
SfaNI GCATC 2 cut(s) 620, 748
SfcI CTRYAG 4 cut(s) 295, 615, 901, 1321
Sfr303I CCGCGG 1 cut(s) 143
SfuI TTCGAA 1 cut(s) 54
SgrBI CCGCGG 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SpeI ACTAGT 1 cut(s) 590
Sse9I AATT 8 cut(s) 428, 477, 781, 916, 968, 1301, 1353, 1409
SspI AATATT 2 cut(s) 211, 1018
SspMI CTAG 3 cut(s) 591, 1209, 1227
SstI GAGCTC 2 cut(s) 66, 150
StyD4I CCNGG 2 cut(s) 571, 1261
TaaI ACNGT 4 cut(s) 273, 282, 1000, 1329
TaqI TCGA 5 cut(s) 42, 54, 124, 132, 225
TasI AATT 8 cut(s) 428, 477, 781, 916, 968, 1301, 1353, 1409
TauI GCSGC 5 cut(s) 8, 139, 142, 190, 423
TfiI GAWTC 8 cut(s) 56, 376, 482, 488, 886, 1003, 1146, 1168
Tru1I TTAA 4 cut(s) 446, 476, 672, 1441
Tru9I TTAA 4 cut(s) 446, 476, 672, 1441
TscAI CASTG 2 cut(s) 118, 808
TseI GCWGC 1 cut(s) 74
TspDTI ATGAA 3 cut(s) 776, 804, 1424
TspGWI ACGGA 2 cut(s) 303, 1009
TspRI CASTG 2 cut(s) 118, 808
XapI RAATTY 3 cut(s) 428, 781, 916
XmiI GTMKAC 1 cut(s) 1253
XspI CTAG 3 cut(s) 591, 1209, 1227
Zsp2I ATGCAT 2 cut(s) 613, 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.