pycom03g15310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005748
Physical Location & Seq
Forward (+)
16618280 .. 16618986
707 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g15310.1

Sequence Viewer

Length: 627 bp
ATGCGGGCAGCATATTACTTACAGAACCACCACTGTGATGAACACTGCCAGATGGTGTATCTTATAGGATGCTTGTCTAAAGGAGTATATAACTGCTGTGAAGAAGAATGTACAGAATTCTCCATAAATGGAAACACAGTTTCATCATGCACCACATGTCAACAACTTCTTTTTCACTCTGTGATAACACACACTACCTTTGTTTCCCAAAGCATAACCCAAGAACGTACATTGATCAGTTCTAGCATCCAACTTACAAGTGTAGGGTCTAAAAAAGGGGTAAACTGCTGTGCCAAAAACTCTGAGACCTTTCAACTCTGGATGTACTCCAAACAACTTAAAAAATGGAGATTGCATTTGTGTAATCCCTCCAATTTTTCCAAATTCAAATCCCGCCCTATAAATCCCAGTCTTCCAGTCTCACCCATTTTTCATCTCCCTCCAATTTTCAATACCCAAAATCCATTTTCTCGATTTCTCTCCATGGCCGACTACGAGCCTCCGTCGTTCTCTCTCGGACTCCACCCTGGTTTAGATTCGCAGCTCCAACCTCCTGCCGCGAACCACTCCACACCCGCCGCAGCTCCAAACTCTTCGCGGGGTTCCAATGCCCACCCGTTGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

23.21

Weight (kDa)

8.65

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 560, 598
AciI CCGC 6 cut(s) 4, 394, 558, 576, 579, 598
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 2 cut(s) 116, 383
AdeI CACNNNGTG 1 cut(s) 181
AfaI GTAC 3 cut(s) 112, 229, 326
AflIII ACRYGT 1 cut(s) 155
AgsI TTSAA 3 cut(s) 314, 388, 451
AjnI CCWGG 1 cut(s) 526
AleI CACNNNNGTG 1 cut(s) 33
AluBI AGCT 2 cut(s) 544, 584
AluI AGCT 2 cut(s) 544, 584
Alw26I GTCTC 2 cut(s) 299, 424
AoxI GGCC 1 cut(s) 486
ApeKI GCWGC 3 cut(s) 8, 541, 581
ApoI RAATTY 2 cut(s) 116, 383
AsuHPI GGTGA 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 404
BbvI GCAGC 3 cut(s) 20, 553, 593
BccI CCATC 1 cut(s) 46
BciT130I CCWGG 1 cut(s) 528
BclI TGATCA 1 cut(s) 234
BcoDI GTCTC 2 cut(s) 299, 424
BfaI CTAG 1 cut(s) 243
BisI GCNGC 5 cut(s) 9, 542, 558, 579, 582
BlsI GCNGC 5 cut(s) 10, 543, 559, 580, 583
Bme1390I CCNGG 1 cut(s) 528
BmiI GGNNCC 1 cut(s) 604
BmrFI CCNGG 1 cut(s) 528
BmrI ACTGGG 1 cut(s) 402
BmsI GCATC 2 cut(s) 59, 255
BmuI ACTGGG 1 cut(s) 402
BpiI GAAGAC 1 cut(s) 404
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 2 cut(s) 483, 526
Bse1I ACTGG 2 cut(s) 408, 416
BseBI CCWGG 1 cut(s) 528
BseDI CCNNGG 2 cut(s) 483, 526
BseGI GGATG 3 cut(s) 74, 246, 327
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 2 cut(s) 408, 416
BseXI GCAGC 3 cut(s) 20, 553, 593
Bsh1236I CGCG 2 cut(s) 560, 598
BshFI GGCC 1 cut(s) 488
BsmAI GTCTC 2 cut(s) 299, 424
BsnI GGCC 1 cut(s) 488
Bso31I GGTCTC 1 cut(s) 299
Bsp1407I TGTACA 1 cut(s) 110
Bsp143I GATC 1 cut(s) 234
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 6 cut(s) 4, 394, 558, 576, 579, 598
BspANI GGCC 1 cut(s) 488
BspCNI CTCAG 1 cut(s) 295
BspFNI CGCG 2 cut(s) 560, 598
BspLI GGNNCC 1 cut(s) 604
BspTNI GGTCTC 1 cut(s) 299
BsrGI TGTACA 1 cut(s) 110
BsrI ACTGG 2 cut(s) 408, 416
BssECI CCNNGG 2 cut(s) 483, 526
BssMI GATC 1 cut(s) 234
BssT1I CCWWGG 1 cut(s) 483
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 2 cut(s) 35, 139
Bst6I CTCTTC 1 cut(s) 598
BstAUI TGTACA 1 cut(s) 110
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 303
BstDSI CCRYGG 1 cut(s) 483
BstF5I GGATG 3 cut(s) 74, 246, 327
BstFNI CGCG 2 cut(s) 560, 598
BstKTI GATC 1 cut(s) 237
BstMAI GTCTC 2 cut(s) 299, 424
BstMBI GATC 1 cut(s) 234
BstNI CCWGG 1 cut(s) 528
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 526
BstUI CGCG 2 cut(s) 560, 598
BstV1I GCAGC 3 cut(s) 20, 553, 593
BstV2I GAAGAC 1 cut(s) 404
BsuRI GGCC 1 cut(s) 488
BtgI CCRYGG 1 cut(s) 483
BtsCI GGATG 3 cut(s) 74, 246, 327
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 2 cut(s) 31, 43
Cac8I GCNNGC 1 cut(s) 6
Csp6I GTAC 3 cut(s) 111, 228, 325
CviAII CATG 3 cut(s) 147, 156, 484
CviJI RGCY 4 cut(s) 488, 499, 544, 584
CviKI_1 RGCY 4 cut(s) 488, 499, 544, 584
CviQI GTAC 3 cut(s) 111, 228, 325
DdeI CTNAG 1 cut(s) 303
DpnI GATC 1 cut(s) 236
DpnII GATC 1 cut(s) 234
DraIII CACNNNGTG 1 cut(s) 181
EaeI YGGCCR 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 598
EarI CTCTTC 1 cut(s) 598
Eco130I CCWWGG 1 cut(s) 483
Eco31I GGTCTC 1 cut(s) 299
EcoRI GAATTC 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 526
EcoT14I CCWWGG 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 483
FaeI CATG 3 cut(s) 150, 159, 487
FatI CATG 3 cut(s) 146, 155, 483
FauI CCCGC 3 cut(s) 401, 583, 591
FbaI TGATCA 1 cut(s) 234
Fnu4HI GCNGC 5 cut(s) 9, 542, 558, 579, 582
FokI GGATG 3 cut(s) 81, 233, 334
Fsp4HI GCNGC 5 cut(s) 9, 542, 558, 579, 582
FspBI CTAG 1 cut(s) 243
GluI GCNGC 5 cut(s) 9, 542, 558, 579, 582
HaeIII GGCC 1 cut(s) 488
Hin1II CATG 3 cut(s) 150, 159, 487
HincII GTYRAC 1 cut(s) 161
HindII GTYRAC 1 cut(s) 161
HinfI GANTC 2 cut(s) 519, 536
HphI GGTGA 1 cut(s) 414
Hpy166II GTNNAC 2 cut(s) 161, 283
Hpy188I TCNGA 2 cut(s) 304, 518
Hpy188III TCNNGA 2 cut(s) 319, 471
Hpy8I GTNNAC 2 cut(s) 161, 283
Hpy99I CGWCG 1 cut(s) 508
HpyCH4III ACNGT 2 cut(s) 35, 139
HpyCH4IV ACGT 1 cut(s) 226
HpyCH4V TGCA 2 cut(s) 150, 355
HpyF3I CTNAG 1 cut(s) 303
HpySE526I ACGT 1 cut(s) 226
Hsp92II CATG 3 cut(s) 150, 159, 487
Ksp22I TGATCA 1 cut(s) 234
Kzo9I GATC 1 cut(s) 234
LmnI GCTCC 2 cut(s) 549, 589
LpnPI CCDG 7 cut(s) 62, 304, 421, 429, 513, 540, 567
Lsp1109I GCAGC 3 cut(s) 20, 553, 593
LweI GCATC 2 cut(s) 59, 255
MaeI CTAG 1 cut(s) 243
MaeII ACGT 1 cut(s) 226
MalI GATC 1 cut(s) 236
MboI GATC 1 cut(s) 234
MboII GAAGA 4 cut(s) 113, 116, 404, 585
MluCI AATT 4 cut(s) 116, 373, 383, 444
MlyI GAGTC 1 cut(s) 513
MmeI TCCRAC 2 cut(s) 274, 571
MnlI CCTC 5 cut(s) 379, 450, 510, 561, 615
MseI TTAA 1 cut(s) 339
MslI CAYNNNNRTG 2 cut(s) 33, 36
MspR9I CCNGG 1 cut(s) 528
MvaI CCWGG 1 cut(s) 528
MvnI CGCG 2 cut(s) 560, 598
NcoI CCATGG 1 cut(s) 483
NdeII GATC 1 cut(s) 234
NlaIII CATG 3 cut(s) 150, 159, 487
NlaIV GGNNCC 1 cut(s) 604
NspI RCATGY 1 cut(s) 159
OliI CACNNNNGTG 1 cut(s) 33
PciI ACATGT 1 cut(s) 155
PfeI GAWTC 1 cut(s) 536
PkrI GCNGC 5 cut(s) 10, 543, 559, 580, 583
PleI GAGTC 1 cut(s) 513
PpsI GAGTC 1 cut(s) 513
PscI ACATGT 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 526
PspGI CCWGG 1 cut(s) 526
PspN4I GGNNCC 1 cut(s) 604
RsaI GTAC 3 cut(s) 112, 229, 326
RsaNI GTAC 3 cut(s) 111, 228, 325
RseI CAYNNNNRTG 2 cut(s) 33, 36
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 5 cut(s) 9, 542, 558, 579, 582
Sau3AI GATC 1 cut(s) 234
SchI GAGTC 1 cut(s) 513
ScrFI CCNGG 1 cut(s) 528
SetI ASST 7 cut(s) 200, 229, 311, 546, 553, 586, 626
SfaNI GCATC 2 cut(s) 59, 255
SmiMI CAYNNNNRTG 2 cut(s) 33, 36
Sse9I AATT 4 cut(s) 116, 373, 383, 444
SsiI CCGC 6 cut(s) 4, 394, 558, 576, 579, 598
SspMI CTAG 1 cut(s) 243
StyD4I CCNGG 1 cut(s) 526
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 2 cut(s) 35, 139
TaiI ACGT 1 cut(s) 229
TaqI TCGA 1 cut(s) 472
TasI AATT 4 cut(s) 116, 373, 383, 444
TatI WGTACW 2 cut(s) 110, 324
TauI GCSGC 2 cut(s) 560, 581
TfiI GAWTC 1 cut(s) 536
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 2 cut(s) 38, 50
TseI GCWGC 3 cut(s) 8, 541, 581
TspDTI ATGAA 3 cut(s) 54, 132, 422
TspGWI ACGGA 1 cut(s) 492
TspRI CASTG 2 cut(s) 38, 50
XapI RAATTY 2 cut(s) 116, 383
XceI RCATGY 1 cut(s) 159
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.